pycom14g18140

Acyl-activating enzyme 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010059
Physical Location & Seq
Reverse (-)
19877419 .. 19884396
6978 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2376 bp
ATGTTTCCTCACCAGCTTAGCTCTCATTTCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTCTAATTATTGGCGACGGCGGCGGGAAGATGATGGCGTATAAGAGCCTGGACGGCGTTACAATTTCCGACATCGAAGATCTGGGGATCGAATCGGGTGCTGCGAAGAGACTCCATGGAAGTTTGACTGATATCATCGCTAATTACGGAGCTGCCACTCCGGAGACGTGGCGCAATATCACTGCGCACGTGCTCAGCCCCGAACTTCCCTTCTCGTTCCACCGGATGTTGTACTACGGCTGCTACAAGGACTTCGGACCCGACCCGCCCGCCTGGATGCCTGACCCGGAGACTGCAGGATTAACAAATGTTGGCCAGCTACTTGAGAGGAGGGGAAATGAGCTCTTGGGTTCTAGATACAAGGATCCCATATTCAGCTTTTCGGATTTTCAGGAATTTTCGGTGTCAAACCCTGAGGTTTATTGGAAAACTGTACTACTAGATGAGATGAATGCATCCTTTTATACACCTCCCCAATGCATTTTACGCGAGAATTTGTCTGGAGATGGGCATTTGTTAGTTCCTGGAGGTCAATGGCTTCCTGGAGCGTTTGGGAATCCGGCCAAAAATTGCTTGATTGTAAATCGTAAGAGGAGCTTGAATGATACCATGGTGATATGGCGCGATGAGGGAAATGATGATCTGCCTTTGAATAAGATGACGCTTAAAGAATTGCGCACAGAGGTTTGGTTAGTTGCACATGCGCTAAAAGCACTGGGTTTGGAGAAAGGCTCTGCAATCGCAATTGATATGCCGATGCATGTCAATTCCATAGTTATTTACCTGGCTATTGTTCTGGCAGGATGTGTCGTTGTATCAATCGCTGATAGTTTTGCTCCTCCTGAAATATCAACAAGGCTTAAAATATCGGATGCAAAAGCCATATTTACCCAGGATCTAATAAAACGCGGCGAGAAAAGTCTGCCCTTGTACAGTAAAATTGTGGCAGCAGAGTCTCCTATGGCAATCGTTATTCTTACCAAAGGTTCCAGTTCTAGCATGAAATTGCGTGATGGTGACATTTCCTGGCATGATTTTCTAGAAACAGTAAAAGATTTCAAAGACAATGAATTTGCTGCTGTAGAACAACCCATAGAAGCATTTACAAATATTCTCTTCTCATCTGGAACTACTGGGGATCCAAAGGCAATCCCATGGACCCTTGCAACTCCTTTCAAAGCTGCTGCAGATGCCTGGTGTCACATGGACATCCGTATAGGTGACGTTGTTGCTTGGCCCACAAATCTTGGGTGGATGATGGGCCCTTGGCTGGTATATGCTTCATTGTTAAATGGAGCTTCCATTGCCTTATATAATGGATCTCCACTTGGATCCAGCTTTGCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGTGTAATCCCGAGTATTGTTCGGTCATGGAAAAGTACAAATTCTGTTTCCGGCTATGATTGGTCTGCCATTCGTTGCTTTGGATCCACCGGCGAAGCATCAAATGTAGAGGAATACCTATGGCTGATGGGGAGGGCTCACTACAAGCCTATCATTGAGTACTGTGGTGGTACAGAGATTGGTGGTGGTTTTGTTACTGGGTCGTTTTTGCAGGCTCAGTCGTTGGCTGCTTTTAGCACACCAGCTATGGGCTGCAGTTTGTATATTCTTGGCAGTGATGGTGTTCCTATTCCCCAAAATGAGCCAGGGGTTGGTGAATTGGCGCTTGATCCTATCATGTTTGGAGCTTCTAGTACATTACTGAATGCTAATCATTATGACATTTATTTCAAGGGAATGCCAATTTGGAATGGAAAGGTGTTAAGGAGGCATGGAGATGTGTTTGAACGGACATCTAGAGGTTACTATCATGCGCACGGTCGTGCAGATGATACAATGAATATTGGGGGCATCAAGGTTAGTTCGGTTGAGATTGAACGAATCTGTAATGCAGTTGATAACAATGTTCTCGAGACAGCTGCGATTGGAGTGCCGCTTGCTGGAGGCGGGCCTGAGCAGCTAGTAATTGCTGTTGTATTCAAGAACTCAGATAACCCAACTGCAGACTTGAATCAGTTGAGAATGTCCTTCAATTCAGCCGTGCAGAAGAAACTAAATCCGTTGTTCAAGGTTTCCAAAGTTGTTCCTCTCCCGTCTCTTCCAAGAACAGCAACAAATAAGGTCATGCGAAGGATTTTGCGGCAGCAGTTTGCTCAACCCGACCAAGGTGCTAAGCTTTCGCAGACTTCCCCAAGAAAAAAAAGCCATGACCTCCCTCTCCATCTCCTCCCCCAATTTCACCACCACTTTCCTCGGCCACAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACAS_N AMP-binding AMP-binding_C

Protein Analysis

792

Amino Acids

86.6

Weight (kDa)

6.39

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 242 - 590 3e-43 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 258, 749, 1923
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccII CGCG 3 cut(s) 561, 696, 981
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 8 cut(s) 93, 96, 338, 342, 981, 2042, 2055, 2248
AcoI YGGCCR 3 cut(s) 385, 633, 2363
AcsI RAATTY 4 cut(s) 467, 565, 1142, 1489
AcvI CACGTG 1 cut(s) 262
AfaI GTAC 7 cut(s) 305, 507, 1004, 1486, 1610, 1621, 1804
AfiI CCNNNNNNNGG 5 cut(s) 1342, 1697, 1760, 2048, 2273
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 9 cut(s) 120, 344, 595, 613, 855, 963, 1097, 1265, 1753
AjuI GAANNNNNNNTTGG 2 cut(s) 1837, 1869
AleI CACNNNNGTG 1 cut(s) 1929
Alw21I GWGCWC 2 cut(s) 267, 417
Alw26I GTCTC 6 cut(s) 175, 230, 356, 1032, 2015, 2208
Ama87I CYCGRG 2 cut(s) 1459, 2018
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 6 cut(s) 385, 633, 1307, 1333, 2057, 2363
ApaI GGGCCC 1 cut(s) 1337
ApoI RAATTY 4 cut(s) 467, 565, 1142, 1489
ArsI GACNNNNNNTTYG 2 cut(s) 1127, 1159
AspLEI GCGC 7 cut(s) 246, 259, 696, 750, 778, 1774, 1924
AspS9I GGNCC 6 cut(s) 329, 1230, 1308, 1333, 1334, 2057
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 5 cut(s) 697, 1100, 1304, 1775, 2339
AvaI CYCGRG 2 cut(s) 1459, 2018
AvaII GGWCC 2 cut(s) 329, 1230
AxyI CCTNAGG 1 cut(s) 486
BaeGI GKGCMC 1 cut(s) 1337
BaeI ACNNNNGTAYC 2 cut(s) 669, 702
BalI TGGCCA 1 cut(s) 387
BamHI GGATCC 4 cut(s) 436, 1210, 1403, 1532
BanII GRGCYC 3 cut(s) 417, 1337, 1588
BbrPI CACGTG 1 cut(s) 262
Bbv12I GWGCWC 2 cut(s) 267, 417
BccI CCATC 7 cut(s) 100, 572, 1079, 1324, 1570, 1721, 2337
BceAI ACGGC 4 cut(s) 106, 142, 325, 2132
BcgI CGANNNNNNTGC 8 cut(s) 152, 186, 2009, 2020, 2043, 2054, 2258, 2292
BciT130I CCWGG 9 cut(s) 122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 6 cut(s) 175, 230, 356, 1032, 2015, 2208
BfaI CTAG 7 cut(s) 426, 512, 1068, 1112, 1800, 1905, 2069
BfmI CTRYAG 5 cut(s) 366, 1152, 1257, 1702, 2109
BfoI RGCGCY 1 cut(s) 1775
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 151
BlpI GCTNAGC 3 cut(s) 17, 266, 2280
BmcAI AGTACT 1 cut(s) 1610
Bme18I GGWCC 2 cut(s) 329, 1230
BmeT110I CYCGRG 2 cut(s) 1459, 2018
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 6 cut(s) 329, 1230, 1308, 1333, 1334, 2057
BmiI GGNNCC 8 cut(s) 331, 438, 1060, 1212, 1232, 1335, 1405, 1534
BmrI ACTGGG 3 cut(s) 797, 1215, 1656
BmsI GCATC 8 cut(s) 339, 536, 819, 934, 1252, 1420, 1556, 1968
BmuI ACTGGG 3 cut(s) 797, 1215, 1656
BplI GAGNNNNNCTC 2 cut(s) 788, 820
BpmI CTGGAG 4 cut(s) 594, 618, 636, 2070
Bpu10I CCTNAGC 1 cut(s) 2061
Bpu1102I GCTNAGC 3 cut(s) 17, 266, 2280
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 262
BsaJI CCNNGG 8 cut(s) 187, 681, 963, 1226, 1337, 1754, 2272, 2360
BsaWI WCCGGW 2 cut(s) 232, 294
Bsc4I CCNNNNNNNGG 5 cut(s) 1342, 1697, 1760, 2048, 2273
Bse118I RCCGGY 1 cut(s) 1538
Bse1I ACTGG 4 cut(s) 792, 1062, 1210, 1651
Bse21I CCTNAGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 1374
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 9 cut(s) 122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BseDI CCNNGG 8 cut(s) 187, 681, 963, 1226, 1337, 1754, 2272, 2360
BseGI GGATG 8 cut(s) 303, 354, 527, 881, 949, 1281, 1332, 1435
BseLI CCNNNNNNNGG 5 cut(s) 1342, 1697, 1760, 2048, 2273
BseMI GCAATG 1 cut(s) 1374
BseMII CTCAG 5 cut(s) 280, 477, 1679, 2052, 2109
BseNI ACTGG 4 cut(s) 792, 1062, 1210, 1651
BseRI GAGGAG 4 cut(s) 415, 679, 900, 2324
BseSI GKGCMC 1 cut(s) 1337
BsgI GTGCAG 2 cut(s) 1953, 2171
Bsh1236I CGCG 3 cut(s) 561, 696, 981
Bsh1285I CGRYCG 1 cut(s) 1930
BshFI GGCC 6 cut(s) 387, 635, 1309, 1335, 2059, 2365
BsiEI CGRYCG 1 cut(s) 1930
BsiHKAI GWGCWC 2 cut(s) 267, 417
BsiHKCI CYCGRG 2 cut(s) 1459, 2018
BsiSI CCGG 6 cut(s) 233, 295, 359, 632, 1500, 1539
BslI CCNNNNNNNGG 5 cut(s) 1342, 1697, 1760, 2048, 2273
BsmAI GTCTC 6 cut(s) 175, 230, 356, 1032, 2015, 2208
BsmBI CGTCTC 2 cut(s) 230, 2208
BsmI GAATGC 3 cut(s) 529, 1819, 1851
BsnI GGCC 6 cut(s) 387, 635, 1309, 1335, 2059, 2365
BsoBI CYCGRG 2 cut(s) 1459, 2018
Bsp120I GGGCCC 1 cut(s) 1333
Bsp1286I GDGCHC 4 cut(s) 267, 417, 1337, 1588
Bsp13I TCCGGA 1 cut(s) 232
Bsp1407I TGTACA 1 cut(s) 1002
Bsp1720I GCTNAGC 3 cut(s) 17, 266, 2280
Bsp19I CCATGG 3 cut(s) 187, 681, 1226
BspACI CCGC 8 cut(s) 93, 96, 338, 342, 981, 2042, 2055, 2248
BspANI GGCC 6 cut(s) 387, 635, 1309, 1335, 2059, 2365
BspCNI CTCAG 5 cut(s) 279, 478, 1678, 2053, 2108
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 3 cut(s) 561, 696, 981
BspLI GGNNCC 8 cut(s) 331, 438, 1060, 1212, 1232, 1335, 1405, 1534
BspMAI CTGCAG 4 cut(s) 370, 1261, 1706, 2113
BsrDI GCAATG 1 cut(s) 1374
BsrFI RCCGGY 1 cut(s) 1538
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 4 cut(s) 792, 1062, 1210, 1651
BssAI RCCGGY 1 cut(s) 1538
BssECI CCNNGG 8 cut(s) 187, 681, 963, 1226, 1337, 1754, 2272, 2360
BssT1I CCWWGG 5 cut(s) 187, 681, 1226, 1337, 2272
Bst2UI CCWGG 9 cut(s) 122, 346, 597, 615, 857, 965, 1099, 1267, 1755
Bst4CI ACNGT 5 cut(s) 505, 1007, 1120, 1613, 1928
Bst6I CTCTTC 3 cut(s) 173, 1193, 2211
BstAUI TGTACA 1 cut(s) 1002
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 5 cut(s) 342, 389, 1662, 2046, 2057
BstDEI CTNAG 7 cut(s) 17, 266, 486, 1665, 2061, 2095, 2280
BstDSI CCRYGG 3 cut(s) 187, 681, 1226
BstF5I GGATG 8 cut(s) 303, 354, 527, 881, 949, 1281, 1332, 1435
BstFNI CGCG 3 cut(s) 561, 696, 981
BstH2I RGCGCY 1 cut(s) 1775
BstHHI GCGC 7 cut(s) 246, 259, 696, 750, 778, 1774, 1924
BstMAI GTCTC 6 cut(s) 175, 230, 356, 1032, 2015, 2208
BstMCI CGRYCG 1 cut(s) 1930
BstMWI GCNNNNNNNGC 7 cut(s) 93, 126, 558, 782, 1262, 1376, 2065
BstNI CCWGG 9 cut(s) 122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BstNSI RCATGY 2 cut(s) 776, 836
BstSFI CTRYAG 5 cut(s) 366, 1152, 1257, 1702, 2109
BstSLI GKGCMC 1 cut(s) 1337
BstUI CGCG 3 cut(s) 561, 696, 981
BstX2I RGATCY 7 cut(s) 151, 436, 967, 1210, 1391, 1403, 1532
BstYI RGATCY 7 cut(s) 151, 436, 967, 1210, 1391, 1403, 1532
Bsu36I CCTNAGG 1 cut(s) 486
BsuRI GGCC 6 cut(s) 387, 635, 1309, 1335, 2059, 2365
BtgI CCRYGG 3 cut(s) 187, 681, 1226
BtgZI GCGATG 2 cut(s) 193, 711
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 8 cut(s) 303, 354, 527, 881, 949, 1281, 1332, 1435
BtsI GCAGTG 2 cut(s) 252, 1729
BtsIMutI CAGTG 3 cut(s) 252, 785, 1729
Cac8I GCNNGC 5 cut(s) 342, 389, 1662, 2046, 2057
CfoI GCGC 7 cut(s) 246, 259, 696, 750, 778, 1774, 1924
Cfr10I RCCGGY 1 cut(s) 1538
Cfr13I GGNCC 6 cut(s) 329, 1230, 1308, 1333, 1334, 2057
CseI GACGC 1 cut(s) 742
Csp6I GTAC 7 cut(s) 304, 506, 1003, 1485, 1609, 1620, 1803
CspCI CAANNNNNGTGG 2 cut(s) 1300, 1335
CviQI GTAC 7 cut(s) 304, 506, 1003, 1485, 1609, 1620, 1803
DdeI CTNAG 7 cut(s) 17, 266, 486, 1665, 2061, 2095, 2280
EaeI YGGCCR 3 cut(s) 385, 633, 2363
Eam1104I CTCTTC 3 cut(s) 173, 1193, 2211
EarI CTCTTC 3 cut(s) 173, 1193, 2211
Ecl136II GAGCTC 1 cut(s) 415
Eco130I CCWWGG 5 cut(s) 187, 681, 1226, 1337, 2272
Eco24I GRGCYC 3 cut(s) 417, 1337, 1588
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 329, 1230
Eco53kI GAGCTC 1 cut(s) 415
Eco72I CACGTG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 486
Eco88I CYCGRG 2 cut(s) 1459, 2018
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 1334
EcoRII CCWGG 9 cut(s) 120, 344, 595, 613, 855, 963, 1097, 1265, 1753
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 5 cut(s) 187, 681, 1226, 1337, 2272
EcoT22I ATGCAT 3 cut(s) 529, 554, 834
EcoT38I GRGCYC 3 cut(s) 417, 1337, 1588
ErhI CCWWGG 5 cut(s) 187, 681, 1226, 1337, 2272
Esp3I CGTCTC 2 cut(s) 230, 2208
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FauI CCCGC 4 cut(s) 89, 345, 349, 2048
FokI GGATG 8 cut(s) 310, 361, 514, 888, 956, 1268, 1339, 1442
FriOI GRGCYC 3 cut(s) 417, 1337, 1588
FspAI RTGCGCAY 1 cut(s) 1923
FspBI CTAG 7 cut(s) 426, 512, 1068, 1112, 1800, 1905, 2069
FspI TGCGCA 3 cut(s) 258, 749, 1923
GlaI GCGC 7 cut(s) 245, 258, 695, 749, 777, 1773, 1923
GsuI CTGGAG 4 cut(s) 594, 618, 636, 2070
HaeII RGCGCY 1 cut(s) 1775
HaeIII GGCC 6 cut(s) 387, 635, 1309, 1335, 2059, 2365
HapII CCGG 6 cut(s) 233, 295, 359, 632, 1500, 1539
HgaI GACGC 1 cut(s) 742
HhaI GCGC 7 cut(s) 246, 259, 696, 750, 778, 1774, 1924
Hin6I GCGC 7 cut(s) 244, 257, 694, 748, 776, 1772, 1922
HinP1I GCGC 7 cut(s) 244, 257, 694, 748, 776, 1772, 1922
HindIII AAGCTT 1 cut(s) 2282
HinfI GANTC 6 cut(s) 164, 183, 628, 1025, 1989, 2119
HpaII CCGG 6 cut(s) 233, 295, 359, 632, 1500, 1539
HphI GGTGA 5 cut(s) 697, 1100, 1304, 1775, 2339
Hpy188I TCNGA 5 cut(s) 142, 329, 457, 943, 2098
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 292, 2146, 2232
HpyCH4III ACNGT 5 cut(s) 505, 1007, 1120, 1613, 1928
HpyCH4IV ACGT 3 cut(s) 239, 261, 1296
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 126, 558, 782, 1262, 1376, 2065
HpyF3I CTNAG 7 cut(s) 17, 266, 486, 1665, 2061, 2095, 2280
HpySE526I ACGT 3 cut(s) 239, 261, 1296
HspAI GCGC 7 cut(s) 244, 257, 694, 748, 776, 1772, 1922
Kpn2I TCCGGA 1 cut(s) 232
LmnI GCTCC 6 cut(s) 221, 617, 666, 913, 1367, 1793
LweI GCATC 8 cut(s) 339, 536, 819, 934, 1252, 1420, 1556, 1968
MaeI CTAG 7 cut(s) 426, 512, 1068, 1112, 1800, 1905, 2069
MaeII ACGT 3 cut(s) 239, 261, 1296
MaeIII GTNAC 7 cut(s) 130, 1088, 1271, 1292, 1438, 1642, 1910
MboII GAAGA 6 cut(s) 112, 161, 190, 1180, 2167, 2198
MfeI CAATTG 1 cut(s) 816
MflI RGATCY 7 cut(s) 151, 436, 967, 1210, 1391, 1403, 1532
MhlI GDGCHC 4 cut(s) 267, 417, 1337, 1588
MlsI TGGCCA 1 cut(s) 387
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 2 cut(s) 177, 1034
MmeI TCCRAC 1 cut(s) 165
Mox20I TGGCCA 1 cut(s) 387
Mph1103I ATGCAT 3 cut(s) 529, 554, 834
MroI TCCGGA 1 cut(s) 232
MscI TGGCCA 1 cut(s) 387
MseI TTAA 6 cut(s) 374, 738, 933, 1361, 1871, 2374
MslI CAYNNNNRTG 2 cut(s) 1884, 1929
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 2027
MspI CCGG 6 cut(s) 233, 295, 359, 632, 1500, 1539
MunI CAATTG 1 cut(s) 816
Mva1269I GAATGC 3 cut(s) 529, 1819, 1851
MvaI CCWGG 9 cut(s) 122, 346, 597, 615, 857, 965, 1099, 1267, 1755
MvnI CGCG 3 cut(s) 561, 696, 981
MwoI GCNNNNNNNGC 7 cut(s) 93, 126, 558, 782, 1262, 1376, 2065
NciI CCSGG 1 cut(s) 359
NcoI CCATGG 3 cut(s) 187, 681, 1226
NlaIV GGNNCC 8 cut(s) 331, 438, 1060, 1212, 1232, 1335, 1405, 1534
NmeAIII GCCGAG 1 cut(s) 2341
NmuCI GTSAC 3 cut(s) 1088, 1271, 1292
NsbI TGCGCA 3 cut(s) 258, 749, 1923
NsiI ATGCAT 3 cut(s) 529, 554, 834
NspI RCATGY 2 cut(s) 776, 836
OliI CACNNNNGTG 1 cut(s) 1929
PaeR7I CTCGAG 1 cut(s) 2018
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PctI GAATGC 3 cut(s) 529, 1819, 1851
PfeI GAWTC 4 cut(s) 164, 628, 1989, 2119
PflMI CCANNNNNTGG 1 cut(s) 1760
PfoI TCCNGGA 2 cut(s) 595, 613
PleI GAGTC 2 cut(s) 177, 1033
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 177, 1033
Ppu21I YACGTR 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 9 cut(s) 120, 344, 595, 613, 855, 963, 1097, 1265, 1753
PspCI CACGTG 1 cut(s) 262
PspGI CCWGG 9 cut(s) 120, 344, 595, 613, 855, 963, 1097, 1265, 1753
PspN4I GGNNCC 8 cut(s) 331, 438, 1060, 1212, 1232, 1335, 1405, 1534
PspOMI GGGCCC 1 cut(s) 1333
PspPI GGNCC 6 cut(s) 329, 1230, 1308, 1333, 1334, 2057
PstI CTGCAG 4 cut(s) 370, 1261, 1706, 2113
PsuI RGATCY 7 cut(s) 151, 436, 967, 1210, 1391, 1403, 1532
PvuII CAGCTG 1 cut(s) 2027
RsaI GTAC 7 cut(s) 305, 507, 1004, 1486, 1610, 1621, 1804
RsaNI GTAC 7 cut(s) 304, 506, 1003, 1485, 1609, 1620, 1803
RseI CAYNNNNRTG 2 cut(s) 1884, 1929
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 6 cut(s) 374, 738, 933, 1361, 1871, 2374
Sau96I GGNCC 6 cut(s) 329, 1230, 1308, 1333, 1334, 2057
ScaI AGTACT 1 cut(s) 1610
SchI GAGTC 2 cut(s) 177, 1034
SduI GDGCHC 4 cut(s) 267, 417, 1337, 1588
SfaNI GCATC 8 cut(s) 339, 536, 819, 934, 1252, 1420, 1556, 1968
SfcI CTRYAG 5 cut(s) 366, 1152, 1257, 1702, 2109
Sfr274I CTCGAG 1 cut(s) 2018
SgrAI CRCCGGYG 1 cut(s) 1538
SinI GGWCC 2 cut(s) 329, 1230
SlaI CTCGAG 1 cut(s) 2018
SmiMI CAYNNNNRTG 2 cut(s) 1884, 1929
SmlI CTYRAG 2 cut(s) 395, 2018
SmoI CTYRAG 2 cut(s) 395, 2018
SsiI CCGC 8 cut(s) 93, 96, 338, 342, 981, 2042, 2055, 2248
SspI AATATT 2 cut(s) 1183, 1951
SspMI CTAG 7 cut(s) 426, 512, 1068, 1112, 1800, 1905, 2069
SstI GAGCTC 1 cut(s) 417
StyI CCWWGG 5 cut(s) 187, 681, 1226, 1337, 2272
TaaI ACNGT 5 cut(s) 505, 1007, 1120, 1613, 1928
TaiI ACGT 3 cut(s) 242, 264, 1299
TaqI TCGA 3 cut(s) 147, 162, 2019
TaqII GACCGA 1 cut(s) 1461
TatI WGTACW 6 cut(s) 303, 505, 1002, 1484, 1608, 1802
TauI GCSGC 4 cut(s) 96, 984, 2044, 2251
TfiI GAWTC 4 cut(s) 164, 628, 1989, 2119
Tru1I TTAA 6 cut(s) 374, 738, 933, 1361, 1871, 2374
Tru9I TTAA 6 cut(s) 374, 738, 933, 1361, 1871, 2374
TscAI CASTG 3 cut(s) 259, 792, 1729
TseFI GTSAC 3 cut(s) 1088, 1271, 1292
Tsp45I GTSAC 3 cut(s) 1088, 1271, 1292
TspDTI ATGAA 5 cut(s) 536, 1088, 1155, 1344, 1961
TspGWI ACGGA 4 cut(s) 234, 1274, 1912, 2157
TspRI CASTG 3 cut(s) 259, 792, 1729
Van91I CCANNNNNTGG 1 cut(s) 1760
VpaK11BI GGWCC 2 cut(s) 329, 1230
XapI RAATTY 4 cut(s) 467, 565, 1142, 1489
XbaI TCTAGA 3 cut(s) 425, 1111, 1904
XceI RCATGY 2 cut(s) 776, 836
XhoI CTCGAG 1 cut(s) 2018
XspI CTAG 7 cut(s) 426, 512, 1068, 1112, 1800, 1905, 2069
ZrmI AGTACT 1 cut(s) 1610
Zsp2I ATGCAT 3 cut(s) 529, 554, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.