pycom14g18480

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014089
Physical Location & Seq
Reverse (-)
20125527 .. 20126432
906 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGAAATCTTCAAGATCAATCTCTCCCTTCAGGCTCTGCTCCCTCCTCCGTCGCAAGAAGAACCCGAATCTCGCTTTCCAACTCTTTCAAAATCTCAACCCCGATACGGAAACTCCGCCTGGACAGCCCTTTCGCTACTCTCTCCTTTCCTACGACCTCATCATCACCAAGCTCGGCCGCGCGAAAATGTTGGACCAGATGGAGCAAATCCTCCACCAAATGAAGCAAGAAACCCGTTTCTACCCGCCGCGGTTGAAAAAAGCATTTAAGTTGAAATATGATATGGTGGTGATTCATGGCGTTGTGCCTAATCTATACGTGTATACCTCTTTGATCAGAGGGCTTTGTAAGATTGGTGAAATGATTTCGGCTTTTAGGCTGAAAGAGGAGATGGCGACGAAGAAAATCAAGCCCGATTCAGTTGTTAATTCAACTTTAATCAGCGGGCTTCACAATGCTGGCCGGAAAGAAGAGGTTTCCGGGCTTTTGGACGAAATGAGATTGTACGGGTGCAAACCAGATACCGTGACTTTTAATGCATTGATTCACGGGTTGTGTAAAGAGAGTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

192

Amino Acids

21.87

Weight (kDa)

9.72

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 100 - 131 3.2e-09 PPR repeat
PPR_2 PF13041 103 - 151 8.3e-11 PPR repeat family
PPR_3 PF13812 126 - 183 3.7e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 138 - 187 7.3e-13 PPR repeat family
PPR_1 PF12854 170 - 188 3.1e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53330 AT1G53330
fragaria_vesca FvH4_5g12530
malus_domestica MD14G1223500.v1.1
prunus_persica Prupe.5G218700_v2.0.a1
pyrus_communis pycom06g18980 pycom14g18480
rosa_chinensis RchiOBHm_Chr7g0184831
rosa_laevigata RLG00000004951
rosa_multiflora Rmu_ssc0000289.1_g000014
rosa_roxburghii Rroxscaffold_3G00269350
rosa_rugosa Rorug06G0465200
rosa_samantha Rh7AG077700 Rh7BG069600 Rh7CG070400 Rh7DG069800
rosa_wichuraiana Rw7G005770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 323
AccII CGCG 3 cut(s) 180, 182, 250
AciI CCGC 6 cut(s) 116, 178, 245, 248, 250, 444
AcoI YGGCCR 2 cut(s) 175, 460
AcuI CTGAAG 1 cut(s) 13
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 106
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 5 cut(s) 12, 89, 256, 274, 432
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 36
AoxI GGCC 2 cut(s) 175, 460
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 157, 301, 368
AvaII GGWCC 1 cut(s) 193
BccI CCATC 2 cut(s) 193, 385
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 333
BcnI CCSGG 1 cut(s) 481
BisI GCNGC 2 cut(s) 178, 248
BlsI GCNGC 2 cut(s) 179, 249
Bme1390I CCNGG 2 cut(s) 120, 481
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmrFI CCNGG 2 cut(s) 120, 481
BpuMI CCSGG 1 cut(s) 481
BsaAI YACGTR 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 248
BseLI CCNNNNNNNGG 1 cut(s) 106
BseRI GAGGAG 2 cut(s) 35, 401
BseX3I CGGCCG 1 cut(s) 175
Bsh1236I CGCG 3 cut(s) 180, 182, 250
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 177, 462
BsiEI CGRYCG 1 cut(s) 178
BsiSI CCGG 2 cut(s) 463, 480
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 2 cut(s) 177, 462
Bsp143I GATC 2 cut(s) 14, 333
BspACI CCGC 6 cut(s) 116, 178, 245, 248, 250, 444
BspANI GGCC 2 cut(s) 177, 462
BspFNI CGCG 3 cut(s) 180, 182, 250
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 2 cut(s) 14, 333
BssNAI GTATAC 1 cut(s) 324
Bst1107I GTATAC 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 446, 460
BstDSI CCRYGG 1 cut(s) 248
BstFNI CGCG 3 cut(s) 180, 182, 250
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 17, 336
BstMBI GATC 2 cut(s) 14, 333
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 118, 479
BstUI CGCG 3 cut(s) 180, 182, 250
BstZ17I GTATAC 1 cut(s) 324
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 2 cut(s) 177, 462
BtgI CCRYGG 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 446, 460
CaiI CAGNNNCTG 1 cut(s) 36
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 251
Csp6I GTAC 1 cut(s) 505
CviAII CATG 1 cut(s) 296
CviQI GTAC 1 cut(s) 505
DpnI GATC 2 cut(s) 16, 335
DpnII GATC 2 cut(s) 14, 333
EaeI YGGCCR 2 cut(s) 175, 460
EagI CGGCCG 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 105
EclXI CGGCCG 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 541
FaeI CATG 1 cut(s) 299
FaiI YATR 5 cut(s) 279, 284, 297, 316, 324
FatI CATG 1 cut(s) 295
FauI CCCGC 2 cut(s) 252, 437
FbaI TGATCA 1 cut(s) 333
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 2 cut(s) 178, 248
Fsp4HI GCNGC 2 cut(s) 178, 248
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 178, 248
HaeIII GGCC 2 cut(s) 177, 462
HapII CCGG 2 cut(s) 463, 480
HhaI GCGC 1 cut(s) 182
Hin1II CATG 1 cut(s) 299
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 4 cut(s) 67, 292, 416, 544
HpaII CCGG 2 cut(s) 463, 480
HphI GGTGA 3 cut(s) 157, 301, 368
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 1 cut(s) 324
Hpy99I CGWCG 2 cut(s) 54, 400
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 513, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 1 cut(s) 299
HspAI GCGC 1 cut(s) 180
Ksp22I TGATCA 1 cut(s) 333
KspI CCGCGG 1 cut(s) 251
Kzo9I GATC 2 cut(s) 14, 333
LmnI GCTCC 2 cut(s) 44, 202
LpnPI CCDG 8 cut(s) 16, 105, 132, 209, 444, 476, 493, 531
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 526
MalI GATC 2 cut(s) 16, 335
MboI GATC 2 cut(s) 14, 333
MboII GAAGA 3 cut(s) 70, 412, 482
MluCI AATT 1 cut(s) 427
MmeI TCCRAC 2 cut(s) 103, 171
MnlI CCTC 8 cut(s) 53, 56, 167, 221, 332, 337, 379, 466
Mph1103I ATGCAT 1 cut(s) 541
MseI TTAA 4 cut(s) 267, 426, 437, 534
MspA1I CMGCKG 2 cut(s) 250, 444
MspI CCGG 2 cut(s) 463, 480
MspR9I CCNGG 2 cut(s) 120, 481
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 3 cut(s) 180, 182, 250
MwoI GCNNNNNNNGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 481
NdeII GATC 2 cut(s) 14, 333
NlaIII CATG 1 cut(s) 299
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 526
NsiI ATGCAT 1 cut(s) 541
PfeI GAWTC 4 cut(s) 67, 292, 416, 544
PkrI GCNGC 2 cut(s) 179, 249
Ppu21I YACGTR 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacII CCGCGG 1 cut(s) 251
SaqAI TTAA 4 cut(s) 267, 426, 437, 534
SatI GCNGC 2 cut(s) 178, 248
Sau3AI GATC 2 cut(s) 14, 333
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 2 cut(s) 120, 481
SetI ASST 5 cut(s) 159, 174, 321, 329, 477
Sfr303I CCGCGG 1 cut(s) 251
SgrBI CCGCGG 1 cut(s) 251
SinI GGWCC 1 cut(s) 193
Sse9I AATT 1 cut(s) 427
SsiI CCGC 6 cut(s) 116, 178, 245, 248, 250, 444
StyD4I CCNGG 2 cut(s) 118, 479
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 1 cut(s) 321
TasI AATT 1 cut(s) 427
TauI GCSGC 2 cut(s) 180, 250
TfiI GAWTC 4 cut(s) 67, 292, 416, 544
Tru1I TTAA 4 cut(s) 267, 426, 437, 534
Tru9I TTAA 4 cut(s) 267, 426, 437, 534
TseFI GTSAC 1 cut(s) 526
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 3 cut(s) 17, 236, 284
TspGWI ACGGA 2 cut(s) 38, 122
VpaK11BI GGWCC 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.