pycom14g18690

malate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009313
Physical Location & Seq
Reverse (-)
20269726 .. 20271628
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
ATGGATCTGCACAACTTGGTGGTAAAAAATTTCCTGGGGGTTGCAAAATCATTGGAAGGTGTGGTTAACAATTACCTTAATTGCGTTGAATATGAGAGGGTCCCTTCCAAGATTCTTACATATCAAGCTTCTGATGATCCACTCTATAGTGGCTACAGATCTGCTGTGGAATCTACAAGCCAAGAGGACACTCTGATGGGATTCGCTATCTGGGAGCCACCGCATGGTCGATACAGAATGCTTAGATATCCATGGAAGAACTATGTAAAAGTGGGTGGTGCGCTGAGGCATTGTGCTTTTATGGTTATGGCATTGCACGGATGTATACTTTCTGAAATACAGGCTCCTGCTGAGCGAAGACAAGTATTTCGTAGGGAGCTTCAAAGGGTAGGATGCGAAGGTGCTAAAGTGTTACGTGAACTTGGAAACAAACTGAAAACGATGGAGAAAATAGGTCCCATAGATATACTCAATGAAGTGCACGAGGCTGCAGAAGACTTACAAAAGAAAGTTGACCAGAAGTCATACCTGCTTGTTAATTCAGAAAGCTGGGAGATTGGAAGCCGGCCAAAGGAGCTGGGAGAACCTCAAGACTTATTGAATGCCGACGATGACGAAAATTATTTCCGGGAATACAAGTCCCTTAGTGAAGCTGTACTTGATCTCAGATCTTTTCCTGTGCCACAAAGTTGGGATAGCCAGATGCCCGTGAAGCCAAATGGCACGGGCTCTGTAACAACTGATGCCAACCACTCGAACTTGCCTGTAGGTGTTTCCTCAGGAAGCATGTTCATGAAACAGATTTCATGGCCTGCAGGACTTAAATTTAAGGGTGATGAACCGCCAGCAGCGGAGGAATCCAACACATATGAAAATGCTAGTCAATTGTCGTTGGCCACATTTACATCCCTGTTGATAGAGTTTGTTGCAAGGCTTCAAAATCTTGTTGACGCATTTCAAGAATTAAGTGAGAAAGCACATTTCAAGGAACCTATCGAACCACCAGAAGTATTAGAGCCAGCTCGCGGAAGGTTTTGGATCAGATTGTTCAATTGTCTGAAATCCTGA

Protein Analysis

356

Amino Acids

40.13

Weight (kDa)

5.45

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALMT PF11744 2 - 323 8.1e-94 Aluminium activated malate transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 537
AccB7I CCANNNNNTGG 1 cut(s) 224
AccI GTMKAC 1 cut(s) 325
AccII CGCG 1 cut(s) 1026
AciI CCGC 4 cut(s) 221, 842, 851, 1026
AclWI GGATC 3 cut(s) 12, 131, 1046
AcoI YGGCCR 2 cut(s) 566, 894
AcsI RAATTY 2 cut(s) 28, 824
AfaI GTAC 1 cut(s) 657
AfiI CCNNNNNNNGG 3 cut(s) 224, 571, 1025
AgsI TTSAA 7 cut(s) 89, 383, 601, 938, 959, 985, 1051
AhdI GACNNNNNGTC 1 cut(s) 520
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 6 cut(s) 128, 379, 549, 577, 653, 1022
AluI AGCT 6 cut(s) 128, 379, 549, 577, 653, 1022
Alw21I GWGCWC 1 cut(s) 483
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 3 cut(s) 12, 131, 1046
AoxI GGCC 3 cut(s) 566, 809, 894
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 2 cut(s) 488, 848
ApoI RAATTY 2 cut(s) 28, 824
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 100, 455
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 2 cut(s) 100, 455
AxyI CCTNAGG 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 483
BalI TGGCCA 1 cut(s) 896
BanII GRGCYC 1 cut(s) 731
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 2 cut(s) 364, 501
Bbv12I GWGCWC 1 cut(s) 483
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 475, 860
BccI CCATC 2 cut(s) 190, 436
BciT130I CCWGG 1 cut(s) 35
BcnI CCSGG 1 cut(s) 629
BfaI CTAG 1 cut(s) 879
BfmI CTRYAG 5 cut(s) 145, 154, 489, 765, 813
BfuAI ACCTGC 1 cut(s) 537
BglII AGATCT 2 cut(s) 158, 668
BisI GCNGC 2 cut(s) 489, 849
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 2 cut(s) 490, 850
Bme1390I CCNGG 2 cut(s) 35, 629
Bme18I GGWCC 2 cut(s) 100, 455
BmeRI GACNNNNNGTC 1 cut(s) 520
BmgT120I GGNCC 2 cut(s) 100, 455
BmiI GGNNCC 6 cut(s) 101, 102, 216, 345, 457, 990
BmrFI CCNGG 2 cut(s) 35, 629
BmsI GCATC 3 cut(s) 383, 693, 733
BpiI GAAGAC 2 cut(s) 364, 501
Bpu10I CCTNAGC 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 629
BsaAI YACGTR 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 34, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 571, 1025
Bse118I RCCGGY 1 cut(s) 564
Bse21I CCTNAGG 1 cut(s) 778
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 34, 251
BseGI GGATG 3 cut(s) 326, 398, 905
BseLI CCNNNNNNNGG 3 cut(s) 224, 571, 1025
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 4 cut(s) 275, 342, 679, 792
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 2 cut(s) 475, 860
BseYI CCCAGC 2 cut(s) 549, 577
Bsh1236I CGCG 1 cut(s) 1026
BshFI GGCC 3 cut(s) 568, 811, 896
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 2 cut(s) 565, 628
BslFI GGGAC 3 cut(s) 86, 441, 625
BslI CCNNNNNNNGG 3 cut(s) 224, 571, 1025
BsmFI GGGAC 3 cut(s) 86, 441, 625
BsmI GAATGC 2 cut(s) 243, 607
BsnI GGCC 3 cut(s) 568, 811, 896
Bsp1286I GDGCHC 2 cut(s) 483, 731
Bsp143I GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 251
BspACI CCGC 4 cut(s) 221, 842, 851, 1026
BspANI GGCC 3 cut(s) 568, 811, 896
BspCNI CTCAG 4 cut(s) 276, 343, 678, 791
BspFNI CGCG 1 cut(s) 1026
BspHI TCATGA 1 cut(s) 792
BspLI GGNNCC 6 cut(s) 101, 102, 216, 345, 457, 990
BspMAI CTGCAG 2 cut(s) 493, 817
BspMI ACCTGC 1 cut(s) 537
BspPI GGATC 3 cut(s) 12, 131, 1046
BsrDI GCAATG 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 564
BssECI CCNNGG 2 cut(s) 34, 251
BssMI GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
BssNAI GTATAC 1 cut(s) 326
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 251
Bst1107I GTATAC 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 5 cut(s) 566, 813, 846, 1020, 1024
BstDEI CTNAG 6 cut(s) 242, 284, 351, 644, 665, 778
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 3 cut(s) 326, 398, 905
BstFNI CGCG 1 cut(s) 1026
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 6 cut(s) 7, 139, 161, 664, 671, 1041
BstMBI GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
BstMWI GCNNNNNNNGC 2 cut(s) 574, 712
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 790
BstSCI CCNGG 2 cut(s) 33, 627
BstSFI CTRYAG 5 cut(s) 145, 154, 489, 765, 813
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 1 cut(s) 1026
BstV1I GCAGC 2 cut(s) 475, 860
BstV2I GAAGAC 2 cut(s) 364, 501
BstX2I RGATCY 3 cut(s) 4, 158, 668
BstXI CCANNNNNNTGG 1 cut(s) 690
BstYI RGATCY 3 cut(s) 4, 158, 668
BstZ17I GTATAC 1 cut(s) 326
Bsu36I CCTNAGG 1 cut(s) 778
BsuRI GGCC 3 cut(s) 568, 811, 896
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 3 cut(s) 326, 398, 905
BveI ACCTGC 1 cut(s) 537
Cac8I GCNNGC 5 cut(s) 566, 813, 846, 1020, 1024
CciI TCATGA 1 cut(s) 792
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 564
Cfr13I GGNCC 2 cut(s) 100, 455
CseI GACGC 1 cut(s) 959
Csp6I GTAC 1 cut(s) 656
CviAII CATG 5 cut(s) 224, 252, 787, 793, 807
CviQI GTAC 1 cut(s) 656
DdeI CTNAG 6 cut(s) 242, 284, 351, 644, 665, 778
DpnI GATC 6 cut(s) 6, 138, 160, 663, 670, 1040
DpnII GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
DriI GACNNNNNGTC 1 cut(s) 520
EaeI YGGCCR 2 cut(s) 566, 894
Eam1105I GACNNNNNGTC 1 cut(s) 520
Eco130I CCWWGG 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 731
Eco32I GATATC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 100, 455
Eco81I CCTNAGG 1 cut(s) 778
EcoO109I RGGNCCY 2 cut(s) 100, 455
EcoRII CCWGG 1 cut(s) 33
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 5 cut(s) 227, 255, 790, 796, 810
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 3 cut(s) 86, 441, 625
FatI CATG 5 cut(s) 223, 251, 786, 792, 806
FauNDI CATATG 1 cut(s) 868
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 2 cut(s) 489, 849
FokI GGATG 3 cut(s) 333, 405, 892
FriOI GRGCYC 1 cut(s) 731
Fsp4HI GCNGC 2 cut(s) 489, 849
FspBI CTAG 1 cut(s) 879
GlaI GCGC 1 cut(s) 282
GluI GCNGC 2 cut(s) 489, 849
GsaI CCCAGC 2 cut(s) 553, 581
HaeIII GGCC 3 cut(s) 568, 811, 896
HapII CCGG 2 cut(s) 565, 628
HgaI GACGC 1 cut(s) 959
HhaI GCGC 1 cut(s) 283
Hin1II CATG 5 cut(s) 227, 255, 790, 796, 810
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 3 cut(s) 67, 514, 949
HindII GTYRAC 3 cut(s) 67, 514, 949
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 4 cut(s) 112, 170, 201, 857
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 2 cut(s) 565, 628
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 6 cut(s) 67, 326, 419, 481, 514, 949
Hpy188I TCNGA 7 cut(s) 133, 195, 334, 544, 668, 1043, 1059
Hpy188III TCNNGA 5 cut(s) 590, 780, 793, 959, 1065
Hpy8I GTNNAC 6 cut(s) 67, 326, 419, 481, 514, 949
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 4 cut(s) 50, 114, 392, 1023
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 7 cut(s) 10, 44, 316, 481, 491, 815, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 574, 712
HpyF3I CTNAG 6 cut(s) 242, 284, 351, 644, 665, 778
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 5 cut(s) 227, 255, 790, 796, 810
HspAI GCGC 1 cut(s) 281
KflI GGGWCCC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 564
KroNI GCCGGC 1 cut(s) 566
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
LmnI GCTCC 4 cut(s) 214, 349, 376, 574
Lsp1109I GCAGC 2 cut(s) 475, 860
LweI GCATC 3 cut(s) 383, 693, 733
MaeI CTAG 1 cut(s) 879
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 2 cut(s) 411, 733
MalI GATC 6 cut(s) 6, 138, 160, 663, 670, 1040
MboI GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
MboII GAAGA 3 cut(s) 268, 369, 506
MfeI CAATTG 2 cut(s) 884, 1051
MflI RGATCY 3 cut(s) 4, 158, 668
MhlI GDGCHC 2 cut(s) 483, 731
MlsI TGGCCA 1 cut(s) 896
MluCI AATT 9 cut(s) 28, 70, 79, 538, 619, 824, 884, 962, 1051
MluNI TGGCCA 1 cut(s) 896
MmeI TCCRAC 1 cut(s) 885
MnlI CCTC 7 cut(s) 90, 178, 279, 478, 597, 787, 847
Mox20I TGGCCA 1 cut(s) 896
MroNI GCCGGC 1 cut(s) 564
MscI TGGCCA 1 cut(s) 896
MseI TTAA 6 cut(s) 66, 78, 537, 822, 828, 965
MslI CAYNNNNRTG 2 cut(s) 194, 791
Msp20I TGGCCA 1 cut(s) 896
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 2 cut(s) 565, 628
MspR9I CCNGG 2 cut(s) 35, 629
MunI CAATTG 2 cut(s) 884, 1051
Mva1269I GAATGC 2 cut(s) 243, 607
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 1026
MwoI GCNNNNNNNGC 2 cut(s) 574, 712
NaeI GCCGGC 1 cut(s) 566
NciI CCSGG 1 cut(s) 629
NcoI CCATGG 1 cut(s) 251
NdeI CATATG 1 cut(s) 868
NdeII GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
NgoMIV GCCGGC 1 cut(s) 564
NlaIII CATG 5 cut(s) 227, 255, 790, 796, 810
NlaIV GGNNCC 6 cut(s) 101, 102, 216, 345, 457, 990
NspI RCATGY 1 cut(s) 790
PagI TCATGA 1 cut(s) 792
PctI GAATGC 2 cut(s) 243, 607
PdiI GCCGGC 1 cut(s) 566
PfeI GAWTC 4 cut(s) 112, 170, 201, 857
PflMI CCANNNNNTGG 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 627
PkrI GCNGC 2 cut(s) 490, 850
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 2 cut(s) 100, 455
Psp5II RGGWCCY 2 cut(s) 100, 455
Psp6I CCWGG 1 cut(s) 33
PspFI CCCAGC 2 cut(s) 549, 577
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 6 cut(s) 101, 102, 216, 345, 457, 990
PspPI GGNCC 2 cut(s) 100, 455
PspPPI RGGWCCY 2 cut(s) 100, 455
PstI CTGCAG 2 cut(s) 493, 817
PsuI RGATCY 3 cut(s) 4, 158, 668
RsaI GTAC 1 cut(s) 657
RsaNI GTAC 1 cut(s) 656
RseI CAYNNNNRTG 2 cut(s) 194, 791
SaqAI TTAA 6 cut(s) 66, 78, 537, 822, 828, 965
SatI GCNGC 2 cut(s) 489, 849
Sau3AI GATC 6 cut(s) 4, 136, 158, 661, 668, 1038
Sau96I GGNCC 2 cut(s) 100, 455
SbfI CCTGCAGG 1 cut(s) 817
ScrFI CCNGG 2 cut(s) 35, 629
SdaI CCTGCAGG 1 cut(s) 817
SduI GDGCHC 2 cut(s) 483, 731
SfaNI GCATC 3 cut(s) 383, 693, 733
SfcI CTRYAG 5 cut(s) 145, 154, 489, 765, 813
SinI GGWCC 2 cut(s) 100, 455
SmiMI CAYNNNNRTG 2 cut(s) 194, 791
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse8387I CCTGCAGG 1 cut(s) 817
Sse9I AATT 9 cut(s) 28, 70, 79, 538, 619, 824, 884, 962, 1051
SsiI CCGC 4 cut(s) 221, 842, 851, 1026
SspMI CTAG 1 cut(s) 879
StyD4I CCNGG 2 cut(s) 33, 627
StyI CCWWGG 1 cut(s) 251
TaiI ACGT 1 cut(s) 418
TaqI TCGA 3 cut(s) 229, 755, 996
TasI AATT 9 cut(s) 28, 70, 79, 538, 619, 824, 884, 962, 1051
TatI WGTACW 1 cut(s) 655
TfiI GAWTC 4 cut(s) 112, 170, 201, 857
Tru1I TTAA 6 cut(s) 66, 78, 537, 822, 828, 965
Tru9I TTAA 6 cut(s) 66, 78, 537, 822, 828, 965
TseI GCWGC 2 cut(s) 488, 848
TspDTI ATGAA 6 cut(s) 489, 781, 795, 809, 852, 885
TspGWI ACGGA 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 224
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 2 cut(s) 100, 455
XapI RAATTY 2 cut(s) 28, 824
XceI RCATGY 1 cut(s) 790
XmiI GTMKAC 1 cut(s) 325
XspI CTAG 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.