Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0013291

Physical Location & Seq

Genomic Coordinates Reverse (-)

20810020 .. 20813388

Gene Structure 3369 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGTTCCAAAACTCCGCACCGCGCTACACCGGCTATTCGTACCCTCCCACCACCTATATATACCGCGCGCTTTCAAATCCACACTTCTCATATTTTCCAAAATTCCAAAAATGACCCCCAAGCTCACCGAGGCCTATGCCTGCGTCCCGTCAACGGAGCGAGGCCGTGGGATTCTGATCTCCGGCGACAACAAATCCGACAAGCTCCTCTACACCAATGGCAGATCCGTAATTATCATGAGCCTCCGGAACCCCCTCGACGTCGCCGTTTACGCCGAGCATGCCTACCCCGTCACCGTCGCTAGGTTCTCCCCCAACGGCGAGTGGATCGCGTCCGCCGACGTCTCCGGCACCGTCCGGATCTGGGGGACCCACAACGACTTCGTGTTGAAGAACGAGTTCAAGGTCCTTTCGGGTCGGATCGACGACCTCCAGTGGTCCCCCGACGGGTTGAGGATCGTTGCTTCCGGCGAGGGCAAAGGAAAATCCTTCGTTCGCGCGTTCATGTGGGATTCGGGCTCAACTGTTGGGGATTTTGATGGGCACTCGAAGCGAGTGTTAAGCTGCGCGTTTAAGCCGACGAGACCCTTTCGAATCGTTACTTGTGGGGAGGATTTCTTGGTGAATTTCTACGAAGGACCGCCTTTCAAATTCAAGCTGTCTCTCAGGGATCATTCAAATTTCGTGAATTGTATAAGGTTCTCTCCAGATGGTAGTAAGTTTATCACTGTAAGCTCAGATAAAAGTGGTATTATTTATGATGGAAAGACTGCCGATAAGATCGGAGAGCTGTCCTCGGAAGATGGCCACAAAGGCAGTATTTATGCTCTTAGCTGGAGTCCTGATGGTAAACAGGTTCTGACTGTGTCTGCCGACAAGTCGGCAAAAGTATGGGAGATGTCTGAGGATAATAATGGGAAGCTGAAGAAAACATTGCCCCCTCCTGGGTCAGGTGGAGTGGATGACATGCTAGTTGGGTGTCTATGGCAGAACGATCATATTGTTACAGTTTCTCTCGGAGGCTTCATTAGTATATTCTCAGCAAGCAACCTTGACAAAGCCCCTCTACTGATATCCGGACACATGAAGAATGTTACTTCATTAGCTGTTCTAAAGAGTGACCCGAAACTTGTGTTGTCTAGTAGCTATGATGGTCTTATTGTTAAATGGATTCAAGGTGCTGGATACGGCGGAAAGTTAAAAAGGAAGGAGAATTCTCAAATAAAGTGTTTAGCAGCAGTTGACAAAGAAATCGTTTCATGTGGATTTGACAATAAGGTTTGGAGAGTTCCTCTCCGCAGTGATCAGTGTGGAGATGCAGAGGCTATTGATATTGGAAGTCAGCCAAATGACATAAGCCTTGCGCTTCTATCTCCTGAACTTGCTTTGGTTTCAACTGATGCAGGAGTTGTCATGCTCCGTGGCACAAAAGTTTTATCAACTATAAACCTTGGGTTCACTGTGACTGCATGTACTATTTCACCAGATGGATGTGAAGCCATTGTTGGTGGGCAGGACGGTAAACTGCATATGTATTCTATAACAGGTGATACACTTAATGAAGAGGCGGTTTTGGAGAAGCATCGGGGACCCATTTCTGTCATCCGTTACTCTCCTGATGTTTCTATGTTTGCATCAGGAGACTTGAACCGAGAAGCTGTCGTCTGGGACCGTGCCTCTCGAGAGATCGAGCTTAGGAACATGCTGTACCATACTGCTCGAATAAACTGCCTTGCTTGGTCTCCTGATAGCACACTGGTTGCAACTGGATCTCTTGACACATGTGTTATCATATATGAAGTCGACAAGCCTCCAGCAAGCCGTGTAACCATTAAGGGTGCACACTTGGGTGGCGTCTACGGCATAACTTTCACTGATGAGGACACTGTGGTAAGCTCTGGCGAGGATGCATTTGTTCGTGTCTGGAGGTTGACTCCGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0005938 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0022411 GO:0030029 GO:0030036 GO:0030042 GO:0030832 GO:0030834 GO:0030836 GO:0030863 GO:0030864 GO:0031461 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0032991 GO:0033043 GO:0042641 GO:0042643 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043243 GO:0043244 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051261 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080008 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0110053 GO:1901879 GO:1901881 GO:1902494 GO:1902903 GO:1902905 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

648

Amino Acids

70.51

Weight (kDa)

7.55

Isoelectric Point (pI)

30.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	75 - 211	6e-09	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	91 - 320	1.9e-15	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	93 - 304	1.1e-30	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	93 - 153	9.2e-06	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	94 - 241	1.9e-08	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	141 - 303	2.2e-10	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	144 - 390	1.9e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	163 - 303	3e-10	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	167 - 305	1.4e-15	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	177 - 302	5.2e-06	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	198 - 391	1.7e-12	WDHD1 first WD40 domain
WDR55	PF24796	217 - 429	9.8e-12	WDR55
Beta-prop_CAF1B_HIR1	PF24105	217 - 305	9.7e-08	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	219 - 303	2.9e-10	WDR3 first beta-propeller domain
EIF3I	PF24805	220 - 303	4.5e-08	EIF3I
Beta-prop_EML_2	PF23414	221 - 303	1.1e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	222 - 393	9.1e-18	WDR5 beta-propeller domain
Beta-prop_IFT122_1st	PF23381	223 - 301	3.9e-06	IFT122 first beta-propeller
Beta-prop_THOC3	PF25174	225 - 304	5.5e-12	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	258 - 414	7.5e-09	TEP-1 second beta-propeller
WD40	PF00400	268 - 298	1.3e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	362 - 557	2.5e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	404 - 642	1.6e-07	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	410 - 562	2.1e-08	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	456 - 602	7.2e-07	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	469 - 602	7.5e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	477 - 561	1.5e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	487 - 643	1.3e-19	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	488 - 641	9.1e-06	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	490 - 583	6.9e-09	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	503 - 643	1.2e-08	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	556 - 644	8.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	568 - 644	4.3e-08	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	571 - 644	1.3e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	571 - 646	1.5e-09	WDR3 first beta-propeller domain
WD40	PF00400	571 - 599	1.1e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01330 AT3G18060
fragaria_vesca	FvH4_5g17110
malus_domestica	MD06G1229500.v1.1 MD14G1234400.v1.1
prunus_persica	Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1
pyrus_communis	pycom06g20050 pycom14g19520
rosa_chinensis	RchiOBHm_Chr7g0178701
rosa_laevigata	RLG00000005421
rosa_multiflora	Rmu_sc0002320.1_g000019
rosa_roxburghii	Rroxscaffold_3G00274660
rosa_rugosa	Rorug06G0418700
rosa_samantha	Rh7AG018100 Rh7BG017600
rosa_wichuraiana	Rw7G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	264, 345
AccB1I	GGYRCC	1 cut(s)	350
AccI	GTMKAC	2 cut(s)	1803, 1857
AccII	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
AccIII	TCCGGA	3 cut(s)	246, 357, 1076
AciI	CCGC	9 cut(s)	16, 21, 65, 336, 641, 1191, 1297, 1568, 1937
AclWI	GGATC	7 cut(s)	219, 335, 368, 428, 464, 678, 1777
AcoI	YGGCCR	1 cut(s)	805
AcsI	RAATTY	5 cut(s)	102, 625, 650, 679, 1213
AcuI	CTGAAG	1 cut(s)	944
AcyI	GRCGYC	3 cut(s)	261, 342, 1854
AfaI	GTAC	3 cut(s)	42, 1474, 1709
AfiI	CCNNNNNNNGG	7 cut(s)	154, 303, 364, 447, 944, 945, 950
AflIII	ACRYGT	1 cut(s)	1781
AgsI	TTSAA	9 cut(s)	76, 391, 403, 649, 655, 678, 1175, 1395, 1648
AjnI	CCWGG	1 cut(s)	943
AjuI	GAANNNNNNNTTGG	2 cut(s)	1488, 1520
AleI	CACNNNNGTG	1 cut(s)	1848
Alw21I	GWGCWC	1 cut(s)	1843
Alw26I	GTCTC	5 cut(s)	349, 577, 666, 1635, 1746
Alw44I	GTGCAC	1 cut(s)	1839
AlwI	GGATC	7 cut(s)	219, 335, 368, 428, 464, 678, 1777
AlwNI	CAGNNNCTG	1 cut(s)	859
Ama87I	CYCGRG	1 cut(s)	1680
Aor13HI	TCCGGA	3 cut(s)	246, 357, 1076
AoxI	GGCC	3 cut(s)	132, 163, 805
ApaLI	GTGCAC	1 cut(s)	1839
ApeKI	GCWGC	2 cut(s)	564, 1235
ApoI	RAATTY	5 cut(s)	102, 625, 650, 679, 1213
Asp700I	GAANNNNTTC	1 cut(s)	398
AspLEI	GCGC	6 cut(s)	25, 69, 71, 500, 569, 1366
AspS9I	GGNCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
AsuHPI	GGTGA	5 cut(s)	118, 286, 634, 1473, 1559
AsuII	TTCGAA	1 cut(s)	592
AvaI	CYCGRG	1 cut(s)	1680
AvaII	GGWCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
BaeGI	GKGCMC	2 cut(s)	546, 1843
BalI	TGGCCA	1 cut(s)	807
BanI	GGYRCC	1 cut(s)	350
BanII	GRGCYC	1 cut(s)	521
Bbv12I	GWGCWC	1 cut(s)	1843
BbvI	GCAGC	2 cut(s)	551, 1247
BccI	CCATC	7 cut(s)	533, 704, 755, 797, 839, 1145, 1481
BceAI	ACGGC	6 cut(s)	150, 251, 334, 1204, 1806, 1876
BcgI	CGANNNNNNTGC	4 cut(s)	119, 153, 1710, 1744
BciT130I	CCWGG	1 cut(s)	945
BciVI	GTATCC	1 cut(s)	1178
BclI	TGATCA	1 cut(s)	1303
BcoDI	GTCTC	5 cut(s)	349, 577, 666, 1635, 1746
BfaI	CTAG	3 cut(s)	303, 971, 1140
BfuI	GTATCC	1 cut(s)	1178
BglI	GCCNNNNNGGC	1 cut(s)	813
BisI	GCNGC	2 cut(s)	565, 1236
BlsI	GCNGC	2 cut(s)	566, 1237
Bme1390I	CCNGG	1 cut(s)	945
Bme18I	GGWCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
BmeT110I	CYCGRG	1 cut(s)	1680
BmgT120I	GGNCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
BmiI	GGNNCC	9 cut(s)	6, 251, 352, 370, 371, 440, 1590, 1591, 1670
BmrFI	CCNGG	1 cut(s)	945
BmsI	GCATC	5 cut(s)	1306, 1390, 1591, 1643, 1897
BplI	GAGNNNNNCTC	7 cut(s)	779, 811, 1276, 1308, 1278, 1310, 1918
BpmI	CTGGAG	4 cut(s)	416, 690, 856, 1797
Bpu10I	CCTNAGC	1 cut(s)	1694
Bpu14I	TTCGAA	1 cut(s)	592
BsaBI	GATNNNNATC	1 cut(s)	176
BsaHI	GRCGYC	3 cut(s)	261, 342, 1854
BsaI	GGTCTC	2 cut(s)	577, 1746
BsaJI	CCNNGG	6 cut(s)	129, 166, 795, 944, 1420, 1450
BsaWI	WCCGGW	3 cut(s)	246, 357, 1076
Bsc4I	CCNNNNNNNGG	7 cut(s)	154, 303, 364, 447, 944, 945, 950
Bse118I	RCCGGY	1 cut(s)	30
Bse1I	ACTGG	3 cut(s)	433, 1761, 1771
Bse3DI	GCAATG	1 cut(s)	932
Bse8I	GATNNNNATC	1 cut(s)	176
BseAI	TCCGGA	3 cut(s)	246, 357, 1076
BseBI	CCWGG	1 cut(s)	945
BseDI	CCNNGG	6 cut(s)	129, 166, 795, 944, 1420, 1450
BseGI	GGATG	4 cut(s)	967, 1496, 1602, 1912
BseJI	GATNNNNATC	1 cut(s)	176
BseLI	CCNNNNNNNGG	7 cut(s)	154, 303, 364, 447, 944, 945, 950
BseMI	GCAATG	1 cut(s)	932
BseMII	CTCAG	4 cut(s)	679, 750, 894, 1053
BseNI	ACTGG	3 cut(s)	433, 1761, 1771
BsePI	GCGCGC	1 cut(s)	67
BseRI	GAGGAG	1 cut(s)	197
BseSI	GKGCMC	2 cut(s)	546, 1843
BseXI	GCAGC	2 cut(s)	551, 1247
Bsh1236I	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
BshFI	GGCC	3 cut(s)	134, 165, 807
BshNI	GGYRCC	1 cut(s)	350
BsiHKAI	GWGCWC	1 cut(s)	1843
BsiHKCI	CYCGRG	1 cut(s)	1680
BsiSI	CCGG	7 cut(s)	31, 183, 247, 348, 358, 468, 1077
BslFI	GGGAC	5 cut(s)	131, 382, 424, 1602, 1682
BslI	CCNNNNNNNGG	7 cut(s)	154, 303, 364, 447, 944, 945, 950
BsmAI	GTCTC	5 cut(s)	349, 577, 666, 1635, 1746
BsmBI	CGTCTC	1 cut(s)	349
BsmFI	GGGAC	5 cut(s)	131, 382, 424, 1602, 1682
BsnI	GGCC	3 cut(s)	134, 165, 807
Bso31I	GGTCTC	2 cut(s)	577, 1746
BsoBI	CYCGRG	1 cut(s)	1680
Bsp119I	TTCGAA	1 cut(s)	592
Bsp1286I	GDGCHC	3 cut(s)	521, 546, 1843
Bsp13I	TCCGGA	3 cut(s)	246, 357, 1076
BspACI	CCGC	9 cut(s)	16, 21, 65, 336, 641, 1191, 1297, 1568, 1937
BspANI	GGCC	3 cut(s)	134, 165, 807
BspCNI	CTCAG	4 cut(s)	678, 749, 895, 1052
BspEI	TCCGGA	3 cut(s)	246, 357, 1076
BspFNI	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
BspHI	TCATGA	1 cut(s)	237
BspLI	GGNNCC	9 cut(s)	6, 251, 352, 370, 371, 440, 1590, 1591, 1670
BspPI	GGATC	7 cut(s)	219, 335, 368, 428, 464, 678, 1777
BspT104I	TTCGAA	1 cut(s)	592
BspT107I	GGYRCC	1 cut(s)	350
BspTNI	GGTCTC	2 cut(s)	577, 1746
BsrDI	GCAATG	1 cut(s)	932
BsrFI	RCCGGY	1 cut(s)	30
BsrI	ACTGG	3 cut(s)	433, 1761, 1771
BssAI	RCCGGY	1 cut(s)	30
BssECI	CCNNGG	6 cut(s)	129, 166, 795, 944, 1420, 1450
BssHII	GCGCGC	1 cut(s)	67
BssNI	GRCGYC	3 cut(s)	261, 342, 1854
BssT1I	CCWWGG	1 cut(s)	1450
Bst2UI	CCWGG	1 cut(s)	945
Bst6I	CTCTTC	1 cut(s)	1557
BstACI	GRCGYC	3 cut(s)	261, 342, 1854
BstBI	TTCGAA	1 cut(s)	592
BstC8I	GCNNGC	5 cut(s)	69, 142, 282, 1045, 1819
BstDEI	CTNAG	6 cut(s)	665, 736, 830, 903, 1039, 1694
BstDSI	CCRYGG	2 cut(s)	166, 1420
BstENI	CCTNNNNNAGG	1 cut(s)	948
BstF5I	GGATG	4 cut(s)	967, 1496, 1602, 1912
BstFNI	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
BstHHI	GCGC	6 cut(s)	25, 69, 71, 500, 569, 1366
BstMAI	GTCTC	5 cut(s)	349, 577, 666, 1635, 1746
BstMWI	GCNNNNNNNGC	6 cut(s)	31, 272, 281, 550, 813, 1860
BstNI	CCWGG	1 cut(s)	945
BstNSI	RCATGY	5 cut(s)	284, 970, 1473, 1705, 1785
BstSCI	CCNGG	1 cut(s)	943
BstSLI	GKGCMC	2 cut(s)	546, 1843
BstUI	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
BstV1I	GCAGC	2 cut(s)	551, 1247
BstX2I	RGATCY	3 cut(s)	224, 360, 1769
BstYI	RGATCY	3 cut(s)	224, 360, 1769
BsuI	GTATCC	1 cut(s)	1178
BsuRI	GGCC	3 cut(s)	134, 165, 807
BtgI	CCRYGG	2 cut(s)	166, 1420
BtsCI	GGATG	4 cut(s)	967, 1496, 1602, 1912
BtsI	GCAGTG	1 cut(s)	1306
BtsIMutI	CAGTG	8 cut(s)	440, 726, 1306, 1313, 1458, 1754, 1872, 1884
Cac8I	GCNNGC	5 cut(s)	69, 142, 282, 1045, 1819
CaiI	CAGNNNCTG	1 cut(s)	859
CciI	TCATGA	1 cut(s)	237
CfoI	GCGC	6 cut(s)	25, 69, 71, 500, 569, 1366
Cfr10I	RCCGGY	1 cut(s)	30
Cfr13I	GGNCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
CseI	GACGC	3 cut(s)	133, 321, 1843
Csp6I	GTAC	3 cut(s)	41, 1473, 1708
CviQI	GTAC	3 cut(s)	41, 1473, 1708
DdeI	CTNAG	6 cut(s)	665, 736, 830, 903, 1039, 1694
EaeI	YGGCCR	1 cut(s)	805
Eam1104I	CTCTTC	1 cut(s)	1557
EarI	CTCTTC	1 cut(s)	1557
EciI	GGCGGA	2 cut(s)	325, 1206
Eco130I	CCWWGG	1 cut(s)	1450
Eco147I	AGGCCT	1 cut(s)	134
Eco24I	GRGCYC	1 cut(s)	521
Eco31I	GGTCTC	2 cut(s)	577, 1746
Eco32I	GATATC	1 cut(s)	1074
Eco47I	GGWCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
Eco57I	CTGAAG	1 cut(s)	944
Eco88I	CYCGRG	1 cut(s)	1680
EcoNI	CCTNNNNNAGG	1 cut(s)	948
EcoO109I	RGGNCCY	3 cut(s)	369, 406, 1589
EcoRI	GAATTC	1 cut(s)	1213
EcoRII	CCWGG	1 cut(s)	943
EcoRV	GATATC	1 cut(s)	1074
EcoT14I	CCWWGG	1 cut(s)	1450
EcoT22I	ATGCAT	1 cut(s)	1912
EcoT38I	GRGCYC	1 cut(s)	521
ErhI	CCWWGG	1 cut(s)	1450
Esp3I	CGTCTC	1 cut(s)	349
FaqI	GGGAC	5 cut(s)	131, 382, 424, 1602, 1682
FauNDI	CATATG	1 cut(s)	1530
FbaI	TGATCA	1 cut(s)	1303
FblI	GTMKAC	2 cut(s)	1803, 1857
Fnu4HI	GCNGC	2 cut(s)	565, 1236
FokI	GGATG	4 cut(s)	974, 1503, 1589, 1919
FriOI	GRGCYC	1 cut(s)	521
Fsp4HI	GCNGC	2 cut(s)	565, 1236
FspBI	CTAG	3 cut(s)	303, 971, 1140
GlaI	GCGC	6 cut(s)	24, 68, 70, 499, 568, 1365
GluI	GCNGC	2 cut(s)	565, 1236
GsuI	CTGGAG	4 cut(s)	416, 690, 856, 1797
HaeIII	GGCC	3 cut(s)	134, 165, 807
HapII	CCGG	7 cut(s)	31, 183, 247, 348, 358, 468, 1077
HgaI	GACGC	3 cut(s)	133, 321, 1843
HhaI	GCGC	6 cut(s)	25, 69, 71, 500, 569, 1366
Hin1I	GRCGYC	3 cut(s)	261, 342, 1854
Hin6I	GCGC	6 cut(s)	23, 67, 69, 498, 567, 1364
HinP1I	GCGC	6 cut(s)	23, 67, 69, 498, 567, 1364
HincII	GTYRAC	4 cut(s)	153, 1243, 1804, 1932
HindII	GTYRAC	4 cut(s)	153, 1243, 1804, 1932
HinfI	GANTC	6 cut(s)	172, 512, 594, 838, 1171, 1933
HpaII	CCGG	7 cut(s)	31, 183, 247, 348, 358, 468, 1077
HphI	GGTGA	5 cut(s)	118, 286, 634, 1473, 1559
Hpy188I	TCNGA	9 cut(s)	177, 199, 420, 739, 785, 799, 861, 904, 1019
Hpy99I	CGWCG	7 cut(s)	263, 266, 302, 344, 428, 449, 583
HpyAV	CCTTC	3 cut(s)	499, 629, 1201
HpyCH4IV	ACGT	2 cut(s)	261, 342
HpyCH4V	TGCA	8 cut(s)	1319, 1403, 1469, 1528, 1634, 1763, 1841, 1910
HpyF10VI	GCNNNNNNNGC	6 cut(s)	31, 272, 281, 550, 813, 1860
HpyF3I	CTNAG	6 cut(s)	665, 736, 830, 903, 1039, 1694
HpySE526I	ACGT	2 cut(s)	261, 342
Hsp92I	GRCGYC	3 cut(s)	261, 342, 1854
HspAI	GCGC	6 cut(s)	23, 67, 69, 498, 567, 1364
KflI	GGGWCCC	2 cut(s)	369, 1589
Kpn2I	TCCGGA	3 cut(s)	246, 357, 1076
Ksp22I	TGATCA	1 cut(s)	1303
LmnI	GCTCC	3 cut(s)	157, 210, 1422
Lsp1109I	GCAGC	2 cut(s)	551, 1247
LweI	GCATC	5 cut(s)	1306, 1390, 1591, 1643, 1897
MaeI	CTAG	3 cut(s)	303, 971, 1140
MaeII	ACGT	2 cut(s)	261, 342
MaeIII	GTNAC	8 cut(s)	292, 598, 1003, 1093, 1118, 1462, 1607, 1825
MboII	GAAGA	5 cut(s)	403, 812, 937, 1099, 1574
MflI	RGATCY	3 cut(s)	224, 360, 1769
MhlI	GDGCHC	3 cut(s)	521, 546, 1843
MlsI	TGGCCA	1 cut(s)	807
MluCI	AATT	7 cut(s)	102, 231, 625, 650, 679, 688, 1213
MluNI	TGGCCA	1 cut(s)	807
MlyI	GAGTC	2 cut(s)	847, 1927
MmeI	TCCRAC	2 cut(s)	222, 398
Mox20I	TGGCCA	1 cut(s)	807
Mph1103I	ATGCAT	1 cut(s)	1912
MroI	TCCGGA	3 cut(s)	246, 357, 1076
MroXI	GAANNNNTTC	1 cut(s)	398
MscI	TGGCCA	1 cut(s)	807
MseI	TTAA	6 cut(s)	560, 573, 1164, 1199, 1557, 1833
MslI	CAYNNNNRTG	1 cut(s)	1848
Msp20I	TGGCCA	1 cut(s)	807
MspI	CCGG	7 cut(s)	31, 183, 247, 348, 358, 468, 1077
MspR9I	CCNGG	1 cut(s)	945
MvaI	CCWGG	1 cut(s)	945
MvnI	CGCG	7 cut(s)	23, 67, 69, 332, 498, 500, 569
MwoI	GCNNNNNNNGC	6 cut(s)	31, 272, 281, 550, 813, 1860
NdeI	CATATG	1 cut(s)	1530
NlaIV	GGNNCC	9 cut(s)	6, 251, 352, 370, 371, 440, 1590, 1591, 1670
NmeAIII	GCCGAG	1 cut(s)	301
NmuCI	GTSAC	3 cut(s)	292, 1118, 1462
NsiI	ATGCAT	1 cut(s)	1912
NspI	RCATGY	5 cut(s)	284, 970, 1473, 1705, 1785
NspV	TTCGAA	1 cut(s)	592
OliI	CACNNNNGTG	1 cut(s)	1848
PaeI	GCATGC	1 cut(s)	284
PaeR7I	CTCGAG	1 cut(s)	1680
PagI	TCATGA	1 cut(s)	237
PauI	GCGCGC	1 cut(s)	67
PceI	AGGCCT	1 cut(s)	134
PciI	ACATGT	1 cut(s)	1781
PcsI	WCGNNNNNNNCGW	2 cut(s)	264, 336
PdmI	GAANNNNTTC	1 cut(s)	398
PfeI	GAWTC	4 cut(s)	172, 512, 594, 1171
PflFI	GACNNNGTC	1 cut(s)	865
PkrI	GCNGC	2 cut(s)	566, 1237
PleI	GAGTC	2 cut(s)	846, 1927
PpsI	GAGTC	2 cut(s)	846, 1927
PpuMI	RGGWCCY	3 cut(s)	369, 406, 1589
PscI	ACATGT	1 cut(s)	1781
Psp5II	RGGWCCY	3 cut(s)	369, 406, 1589
Psp6I	CCWGG	1 cut(s)	943
PspGI	CCWGG	1 cut(s)	943
PspN4I	GGNNCC	9 cut(s)	6, 251, 352, 370, 371, 440, 1590, 1591, 1670
PspPI	GGNCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
PspPPI	RGGWCCY	3 cut(s)	369, 406, 1589
PsrI	GAACNNNNNNTAC	2 cut(s)	1691, 1723
PstNI	CAGNNNCTG	1 cut(s)	859
PsuI	RGATCY	3 cut(s)	224, 360, 1769
PsyI	GACNNNGTC	1 cut(s)	865
PteI	GCGCGC	1 cut(s)	67
RsaI	GTAC	3 cut(s)	42, 1474, 1709
RsaNI	GTAC	3 cut(s)	41, 1473, 1708
RseI	CAYNNNNRTG	1 cut(s)	1848
SalI	GTCGAC	1 cut(s)	1802
SaqAI	TTAA	6 cut(s)	560, 573, 1164, 1199, 1557, 1833
SatI	GCNGC	2 cut(s)	565, 1236
Sau96I	GGNCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
SchI	GAGTC	2 cut(s)	847, 1927
ScrFI	CCNGG	1 cut(s)	945
SduI	GDGCHC	3 cut(s)	521, 546, 1843
SfaNI	GCATC	5 cut(s)	1306, 1390, 1591, 1643, 1897
Sfr274I	CTCGAG	1 cut(s)	1680
SfuI	TTCGAA	1 cut(s)	592
SinI	GGWCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
SlaI	CTCGAG	1 cut(s)	1680
SmiMI	CAYNNNNRTG	1 cut(s)	1848
SmlI	CTYRAG	1 cut(s)	1680
SmoI	CTYRAG	1 cut(s)	1680
SphI	GCATGC	1 cut(s)	284
Sse9I	AATT	7 cut(s)	102, 231, 625, 650, 679, 688, 1213
SseBI	AGGCCT	1 cut(s)	134
SsiI	CCGC	9 cut(s)	16, 21, 65, 336, 641, 1191, 1297, 1568, 1937
SspMI	CTAG	3 cut(s)	303, 971, 1140
StuI	AGGCCT	1 cut(s)	134
StyD4I	CCNGG	1 cut(s)	943
StyI	CCWWGG	1 cut(s)	1450
TaiI	ACGT	2 cut(s)	264, 345
TaqI	TCGA	8 cut(s)	258, 423, 548, 592, 1681, 1689, 1720, 1803
TasI	AATT	7 cut(s)	102, 231, 625, 650, 679, 688, 1213
TatI	WGTACW	1 cut(s)	1472
TfiI	GAWTC	4 cut(s)	172, 512, 594, 1171
Tru1I	TTAA	6 cut(s)	560, 573, 1164, 1199, 1557, 1833
Tru9I	TTAA	6 cut(s)	560, 573, 1164, 1199, 1557, 1833
TscAI	CASTG	8 cut(s)	440, 733, 1306, 1313, 1465, 1761, 1879, 1891
TseFI	GTSAC	3 cut(s)	292, 1118, 1462
TseI	GCWGC	2 cut(s)	564, 1235
Tsp45I	GTSAC	3 cut(s)	292, 1118, 1462
TspDTI	ATGAA	7 cut(s)	493, 1015, 1089, 1100, 1248, 1575, 1812
TspGWI	ACGGA	4 cut(s)	170, 217, 1409, 1595
TspRI	CASTG	8 cut(s)	440, 733, 1306, 1313, 1465, 1761, 1879, 1891
Tth111I	GACNNNGTC	1 cut(s)	865
VneI	GTGCAC	1 cut(s)	1839
VpaK11BI	GGWCC	6 cut(s)	369, 406, 438, 638, 1589, 1669
XagI	CCTNNNNNAGG	1 cut(s)	948
XapI	RAATTY	5 cut(s)	102, 625, 650, 679, 1213
XceI	RCATGY	5 cut(s)	284, 970, 1473, 1705, 1785
XhoI	CTCGAG	1 cut(s)	1680
XmiI	GTMKAC	2 cut(s)	1803, 1857
XmnI	GAANNNNTTC	1 cut(s)	398
XspI	CTAG	3 cut(s)	303, 971, 1140
ZraI	GACGTC	2 cut(s)	262, 343
Zsp2I	ATGCAT	1 cut(s)	1912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.