pycom14g19580

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0017202
Physical Location & Seq
Reverse (-)
20848740 .. 20851172
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1743 bp
ATGGAGAGTTTGAGCCAGCGTTATAGATTAGTGCTAATTCTAATCATATTTGCGGTGGCTTTACCGTCATGTTTCTCGCAAACAACCCGACTCTTCGAGTTTAATGTTGAGTGGAAGAATGTAACTCGTATGTGCAACACAAAGCCACTTCTAACAGTGAATGGAGAGTATCCAGGGCCAACCATTGCTGTCTATGAAGGTGATCATCTCGAGATTAAGGTCACTAATAGCATTGCCGAAAATACCACCATCCATTGGCATGGTGTGAGGCAACTAAGAACAGGATGGGCAGACGGTCCAGCTTATATCACACAATGTCCTATAAGAGGTGGCAAGACCTACACATACAAGTTCACTGTGGAGTACCAAAGAGGCACTCTCTGGTGGCATGCTCATTTTGCCTGGCAACGAGCTTCCATTTATGGCGCTTTCATCATCCATCCGCGCATGCCTTTTCCATTTTCCGCTCCGATTCAAGAAGAATTCCCCATCATCTTTGGTGAATGGTGGAATTCAGATGTAGGTGAAGTGGAAAATGAAATGAAGTATGGAGCTGGACCTAATAGTTCAGATGCTTATACCATCAATGGATTGCCAGGACCATTCTATCCTTGCTCGGACAAAGATACCTTCATCAAAACTGTGGAACATGGAAAAACGTACTTGCTTAGAATTATCAACGCAGCACTCAACGATGAGCTTTTCTTTGCTGTGGCTAATCACACACTAACTGTTGTGGAGATTGATGCCGTGTACACCAAACCATTCACAACCTCAGCGATCATGGTAGGTCCTGGCCAAACCACCAACGTTTTGCTCACTGCAAATCAAGTCCCTGACTCCTCGGGTGCCTTTTTGATGGGGGCCTGGCCTTACCTTACCTCCGTTTTCCCTTTCGACAATTCCACCACAGCCGGTTTCTTGCGTTACAAGAACACAAAGAGTGACAAAACTAAATATCCGCCAAAGGCGCTTAGTACTTTTGCGCCGGAAAAATACAACCTCCCTAAAATGGAAGACACAAAGTTTGTCACTATATTTAATAACAAGCTCAGAAGCCTAGCCTCACCTAAGTATCCGTGCAACGTTCCTAAAACTGTCGACAAGCGCGTGGTTATGGTTATAAGCCTAAATCTCCAAGATTGTCCAGGCAACGAAACCTGCAAGGGTTATGCTGGCAAGAAATTTTTTGCTTCTATGAACAACCAGTCCTTCATTCGTCCCGCCTTGTCGATTTTAGAATCTCATTACAAGAAACTCAGAGGCGCCGAGTACTCCACAGATTTCCCAGAGAAACCTTTGAAGCCGTTTGATTACACCAGTGTGGATCCAGTTTCGGAGAACATGAACACAAATTTTGGCACTAAGATTATGGAAGTGGAGCATGGAACAAACTTAGAAATTGTGCTACAAGGTACAAGTTTTCTTAACACTGAGAACCATCCAATTCATGTGCATGGACACAACTTTTTTATAGTCGGTATGGGGTTTGGGAACTTCAATGCTGCCAAGGATCCGGCAAAGTACAACCTCATTGACCCTCCGGAGAGAAACACGGCGGCAGTTCCAACTGGAGGATGGGCGGCGATTCGATTCAAGGCCGACAATCCTGGAGTTTGGTTCATTCACTGTCACCTTGAAGAGCATACTTCGTGGGGTCTTGCCTCGGGGTTTATTGTCAAGAATGGTCCAAAACCATCTCAGTGTGTGCTTCCTCCCCCTGACGATCTTCCCTTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

581

Amino Acids

65.07

Weight (kDa)

7.25

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 35 - 149 2.9e-43 Multicopper oxidase
Cu-oxidase PF00394 160 - 312 8.8e-40 Multicopper oxidase
Cu-oxidase_2 PF07731 429 - 563 4.2e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18140
fragaria_vesca FvH4_5g17150
malus_domestica MD06G1230100.v1.1 MD14G1234800.v1.1
prunus_persica Prupe.5G231100_v2.0.a1
pyrus_communis pycom14g19580
rosa_chinensis RchiOBHm_Chr7g0178641
rosa_laevigata RLG00000005427
rosa_multiflora Rmu_sc0002320.1_g000007
rosa_rugosa Rorug06G0418700
rosa_samantha Rh7AG017400 Rh7BG017000
rosa_wichuraiana Rw7G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1169
AccB1I GGYRCC 2 cut(s) 848, 1265
AccB7I CCANNNNNTGG 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 2 cut(s) 445, 1110
AccIII TCCGGA 1 cut(s) 1543
AciI CCGC 7 cut(s) 53, 443, 465, 962, 1224, 1559, 1583
AclI AACGTT 2 cut(s) 810, 1086
AclWI GGATC 4 cut(s) 1322, 1335, 1508, 1521
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 4 cut(s) 482, 511, 1184, 1354
AcyI GRCGYC 1 cut(s) 1266
AfaI GTAC 7 cut(s) 365, 662, 755, 979, 1274, 1417, 1526
AfiI CCNNNNNNNGG 4 cut(s) 255, 326, 1012, 1574
AgsI TTSAA 5 cut(s) 476, 1303, 1501, 1597, 1640
AjnI CCWGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
AleI CACNNNNGTG 1 cut(s) 1322
AloI GAACNNNNNNTCC 2 cut(s) 1193, 1225
AluBI AGCT 5 cut(s) 302, 413, 554, 700, 1051
AluI AGCT 5 cut(s) 302, 413, 554, 700, 1051
AlwI GGATC 4 cut(s) 1322, 1335, 1508, 1521
Ama87I CYCGRG 3 cut(s) 209, 844, 1666
Aor13HI TCCGGA 1 cut(s) 1543
AoxI GGCC 5 cut(s) 176, 796, 864, 869, 1599
ApeKI GCWGC 2 cut(s) 683, 1505
ApoI RAATTY 4 cut(s) 482, 511, 1184, 1354
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
AspLEI GCGC 6 cut(s) 428, 447, 973, 988, 1110, 1268
AspS9I GGNCC 7 cut(s) 176, 296, 557, 599, 791, 864, 1688
AsuHPI GGTGA 5 cut(s) 212, 512, 536, 1059, 1625
AvaI CYCGRG 3 cut(s) 209, 844, 1666
AvaII GGWCC 5 cut(s) 296, 557, 599, 791, 1688
BaeI ACNNNNGTAYC 2 cut(s) 1058, 1091
BalI TGGCCA 1 cut(s) 798
BamHI GGATCC 2 cut(s) 1327, 1513
BanI GGYRCC 2 cut(s) 848, 1265
BbsI GAAGAC 1 cut(s) 1023
BbvCI CCTCAGC 1 cut(s) 775
BbvI GCAGC 2 cut(s) 695, 1492
BccI CCATC 9 cut(s) 257, 279, 447, 497, 590, 853, 1449, 1572, 1705
BceAI ACGGC 3 cut(s) 734, 1291, 1572
BciT130I CCWGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
BciVI GTATCC 2 cut(s) 180, 1086
BclI TGATCA 1 cut(s) 202
BfaI CTAG 1 cut(s) 1061
BfoI RGCGCY 3 cut(s) 429, 974, 1269
BfuAI ACCTGC 1 cut(s) 1169
BfuI GTATCC 2 cut(s) 180, 1086
BisI GCNGC 4 cut(s) 684, 1506, 1560, 1584
BlsI GCNGC 4 cut(s) 685, 1507, 1561, 1585
BmcAI AGTACT 2 cut(s) 979, 1274
Bme1390I CCNGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
Bme18I GGWCC 5 cut(s) 296, 557, 599, 791, 1688
BmeT110I CYCGRG 3 cut(s) 209, 844, 1666
BmgT120I GGNCC 7 cut(s) 176, 296, 557, 599, 791, 864, 1688
BmiI GGNNCC 5 cut(s) 850, 865, 1267, 1329, 1515
BmrFI CCNGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
BmsI GCATC 2 cut(s) 562, 736
BpiI GAAGAC 1 cut(s) 1023
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BpmI CTGGAG 2 cut(s) 1593, 1632
Bpu10I CCTNAGC 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 1266
BsaJI CCNNGG 4 cut(s) 173, 843, 1509, 1665
BsaWI WCCGGW 1 cut(s) 1543
BsaXI ACNNNNNCTCC 2 cut(s) 866, 896
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 326, 1012, 1574
Bse118I RCCGGY 1 cut(s) 914
Bse1I ACTGG 4 cut(s) 1207, 1320, 1331, 1576
Bse3DI GCAATG 2 cut(s) 183, 231
BseAI TCCGGA 1 cut(s) 1543
BseBI CCWGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
BseDI CCNNGG 4 cut(s) 173, 843, 1509, 1665
BseGI GGATG 6 cut(s) 249, 290, 435, 439, 1441, 1583
BseLI CCNNNNNNNGG 4 cut(s) 255, 326, 1012, 1574
BseMI GCAATG 2 cut(s) 183, 231
BseMII CTCAG 5 cut(s) 789, 1066, 1273, 1425, 1715
BseNI ACTGG 4 cut(s) 1207, 1320, 1331, 1576
BseRI GAGGAG 1 cut(s) 832
BseXI GCAGC 2 cut(s) 695, 1492
Bsh1236I CGCG 2 cut(s) 445, 1110
BshFI GGCC 5 cut(s) 178, 798, 866, 871, 1601
BshNI GGYRCC 2 cut(s) 848, 1265
BsiHKCI CYCGRG 3 cut(s) 209, 844, 1666
BsiSI CCGG 4 cut(s) 915, 989, 1517, 1544
BslFI GGGAC 2 cut(s) 818, 1206
BslI CCNNNNNNNGG 4 cut(s) 255, 326, 1012, 1574
BsmFI GGGAC 2 cut(s) 818, 1206
BsnI GGCC 5 cut(s) 178, 798, 866, 871, 1601
BsoBI CYCGRG 3 cut(s) 209, 844, 1666
Bsp13I TCCGGA 1 cut(s) 1543
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 5 cut(s) 202, 780, 1327, 1513, 1726
BspACI CCGC 7 cut(s) 53, 443, 465, 962, 1224, 1559, 1583
BspANI GGCC 5 cut(s) 178, 798, 866, 871, 1601
BspCNI CTCAG 5 cut(s) 788, 1065, 1272, 1426, 1714
BspEI TCCGGA 1 cut(s) 1543
BspFNI CGCG 2 cut(s) 445, 1110
BspLI GGNNCC 5 cut(s) 850, 865, 1267, 1329, 1515
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 4 cut(s) 1322, 1335, 1508, 1521
BspQI GCTCTTC 1 cut(s) 1635
BspT107I GGYRCC 2 cut(s) 848, 1265
BsrBI CCGCTC 1 cut(s) 467
BsrDI GCAATG 2 cut(s) 183, 231
BsrFI RCCGGY 1 cut(s) 914
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 4 cut(s) 1207, 1320, 1331, 1576
BssAI RCCGGY 1 cut(s) 914
BssECI CCNNGG 4 cut(s) 173, 843, 1509, 1665
BssMI GATC 5 cut(s) 202, 780, 1327, 1513, 1726
BssNI GRCGYC 1 cut(s) 1266
BssT1I CCWWGG 1 cut(s) 1509
Bst2UI CCWGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
Bst4CI ACNGT 8 cut(s) 66, 157, 296, 358, 643, 733, 1099, 1631
Bst6I CTCTTC 2 cut(s) 98, 1635
BstACI GRCGYC 1 cut(s) 1266
BstAUI TGTACA 1 cut(s) 753
BstC8I GCNNGC 4 cut(s) 17, 390, 449, 1177
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 6 cut(s) 249, 290, 435, 439, 1441, 1583
BstFNI CGCG 2 cut(s) 445, 1110
BstH2I RGCGCY 3 cut(s) 429, 974, 1269
BstHHI GCGC 6 cut(s) 428, 447, 973, 988, 1110, 1268
BstKTI GATC 5 cut(s) 205, 783, 1330, 1516, 1729
BstMBI GATC 5 cut(s) 202, 780, 1327, 1513, 1726
BstMWI GCNNNNNNNGC 2 cut(s) 398, 970
BstNI CCWGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
BstNSI RCATGY 2 cut(s) 392, 451
BstSCI CCNGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
BstUI CGCG 2 cut(s) 445, 1110
BstV1I GCAGC 2 cut(s) 695, 1492
BstV2I GAAGAC 1 cut(s) 1023
BstX2I RGATCY 2 cut(s) 1327, 1513
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 2 cut(s) 1327, 1513
BsuI GTATCC 2 cut(s) 180, 1086
BsuRI GGCC 5 cut(s) 178, 798, 866, 871, 1601
BtsCI GGATG 6 cut(s) 249, 290, 435, 439, 1441, 1583
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 7 cut(s) 162, 354, 819, 1327, 1431, 1627, 1709
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 4 cut(s) 17, 390, 449, 1177
CfoI GCGC 6 cut(s) 428, 447, 973, 988, 1110, 1268
Cfr10I RCCGGY 1 cut(s) 914
Cfr13I GGNCC 7 cut(s) 176, 296, 557, 599, 791, 864, 1688
Csp6I GTAC 7 cut(s) 364, 661, 754, 978, 1273, 1416, 1525
CviQI GTAC 7 cut(s) 364, 661, 754, 978, 1273, 1416, 1525
DinI GGCGCC 1 cut(s) 1267
DpnI GATC 5 cut(s) 204, 782, 1329, 1515, 1728
DpnII GATC 5 cut(s) 202, 780, 1327, 1513, 1726
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 2 cut(s) 98, 1635
EarI CTCTTC 2 cut(s) 98, 1635
EciI GGCGGA 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 1509
Eco47I GGWCC 5 cut(s) 296, 557, 599, 791, 1688
Eco88I CYCGRG 3 cut(s) 209, 844, 1666
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO109I RGGNCCY 2 cut(s) 791, 864
EcoRI GAATTC 2 cut(s) 482, 511
EcoRII CCWGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
EcoT14I CCWWGG 1 cut(s) 1509
EgeI GGCGCC 1 cut(s) 1267
EheI GGCGCC 1 cut(s) 1267
ErhI CCWWGG 1 cut(s) 1509
FaqI GGGAC 2 cut(s) 818, 1206
FauI CCCGC 1 cut(s) 1231
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 1101
Fnu4HI GCNGC 4 cut(s) 684, 1506, 1560, 1584
FokI GGATG 6 cut(s) 236, 297, 422, 426, 1428, 1590
Fsp4HI GCNGC 4 cut(s) 684, 1506, 1560, 1584
FspBI CTAG 1 cut(s) 1061
GlaI GCGC 6 cut(s) 427, 446, 972, 987, 1109, 1267
GluI GCNGC 4 cut(s) 684, 1506, 1560, 1584
GsuI CTGGAG 2 cut(s) 1593, 1632
HaeII RGCGCY 3 cut(s) 429, 974, 1269
HaeIII GGCC 5 cut(s) 178, 798, 866, 871, 1601
HapII CCGG 4 cut(s) 915, 989, 1517, 1544
HhaI GCGC 6 cut(s) 428, 447, 973, 988, 1110, 1268
Hin1I GRCGYC 1 cut(s) 1266
Hin6I GCGC 6 cut(s) 426, 445, 971, 986, 1108, 1266
HinP1I GCGC 6 cut(s) 426, 445, 971, 986, 1108, 1266
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
HinfI GANTC 6 cut(s) 90, 472, 839, 1241, 1588, 1593
HpaII CCGG 4 cut(s) 915, 989, 1517, 1544
HphI GGTGA 5 cut(s) 212, 512, 536, 1059, 1625
Hpy166II GTNNAC 4 cut(s) 354, 754, 756, 1102
Hpy188I TCNGA 7 cut(s) 471, 517, 571, 619, 1055, 1262, 1339
Hpy188III TCNNGA 5 cut(s) 209, 211, 476, 1544, 1681
Hpy8I GTNNAC 4 cut(s) 354, 754, 756, 1102
HpyAV CCTTC 3 cut(s) 191, 640, 1222
HpyCH4III ACNGT 8 cut(s) 66, 157, 296, 358, 643, 733, 1099, 1631
HpyCH4IV ACGT 3 cut(s) 659, 810, 1086
HpyCH4V TGCA 5 cut(s) 135, 824, 1083, 1164, 1456
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 970
HpySE526I ACGT 3 cut(s) 659, 810, 1086
Hsp92I GRCGYC 1 cut(s) 1266
HspAI GCGC 6 cut(s) 426, 445, 971, 986, 1108, 1266
KasI GGCGCC 1 cut(s) 1265
Kpn2I TCCGGA 1 cut(s) 1543
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 5 cut(s) 202, 780, 1327, 1513, 1726
LguI GCTCTTC 1 cut(s) 1635
LmnI GCTCC 3 cut(s) 472, 551, 1381
Lsp1109I GCAGC 2 cut(s) 695, 1492
LweI GCATC 2 cut(s) 562, 736
MaeI CTAG 1 cut(s) 1061
MaeII ACGT 3 cut(s) 659, 810, 1086
MaeIII GTNAC 6 cut(s) 121, 220, 926, 944, 1030, 1631
MalI GATC 5 cut(s) 204, 782, 1329, 1515, 1728
MbiI CCGCTC 1 cut(s) 467
MboI GATC 5 cut(s) 202, 780, 1327, 1513, 1726
MboII GAAGA 6 cut(s) 85, 127, 491, 1028, 1652, 1721
MflI RGATCY 2 cut(s) 1327, 1513
MlsI TGGCCA 1 cut(s) 798
MluCI AATT 9 cut(s) 36, 482, 511, 672, 901, 1184, 1354, 1401, 1446
MluNI TGGCCA 1 cut(s) 798
Mly113I GGCGCC 1 cut(s) 1266
MlyI GAGTC 2 cut(s) 84, 833
MmeI TCCRAC 1 cut(s) 1592
Mox20I TGGCCA 1 cut(s) 798
MroI TCCGGA 1 cut(s) 1543
MscI TGGCCA 1 cut(s) 798
MseI TTAA 4 cut(s) 102, 216, 1041, 1428
MslI CAYNNNNRTG 4 cut(s) 258, 1322, 1455, 1702
Msp20I TGGCCA 1 cut(s) 798
MspI CCGG 4 cut(s) 915, 989, 1517, 1544
MspR9I CCNGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
MvaI CCWGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
MvnI CGCG 2 cut(s) 445, 1110
MwoI GCNNNNNNNGC 2 cut(s) 398, 970
NarI GGCGCC 1 cut(s) 1266
NdeII GATC 5 cut(s) 202, 780, 1327, 1513, 1726
NlaIV GGNNCC 5 cut(s) 850, 865, 1267, 1329, 1515
NmeAIII GCCGAG 1 cut(s) 1294
NmuCI GTSAC 4 cut(s) 220, 944, 1030, 1631
NspI RCATGY 2 cut(s) 392, 451
OliI CACNNNNGTG 1 cut(s) 1322
PaeI GCATGC 2 cut(s) 392, 451
PaeR7I CTCGAG 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 1635
PcsI WCGNNNNNNNCGW 1 cut(s) 1107
PfeI GAWTC 4 cut(s) 472, 1241, 1588, 1593
PflMI CCANNNNNTGG 1 cut(s) 255
PfoI TCCNGGA 1 cut(s) 1609
PkrI GCNGC 4 cut(s) 685, 1507, 1561, 1585
PleI GAGTC 2 cut(s) 84, 833
PluTI GGCGCC 1 cut(s) 1269
PpsI GAGTC 2 cut(s) 84, 833
PpuMI RGGWCCY 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1124
Psp1406I AACGTT 2 cut(s) 810, 1086
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
PspGI CCWGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
PspN4I GGNNCC 5 cut(s) 850, 865, 1267, 1329, 1515
PspPI GGNCC 7 cut(s) 176, 296, 557, 599, 791, 864, 1688
PspPPI RGGWCCY 1 cut(s) 791
PsuI RGATCY 2 cut(s) 1327, 1513
RsaI GTAC 7 cut(s) 365, 662, 755, 979, 1274, 1417, 1526
RsaNI GTAC 7 cut(s) 364, 661, 754, 978, 1273, 1416, 1525
RseI CAYNNNNRTG 4 cut(s) 258, 1322, 1455, 1702
SalI GTCGAC 1 cut(s) 1100
SapI GCTCTTC 1 cut(s) 1635
SaqAI TTAA 4 cut(s) 102, 216, 1041, 1428
SatI GCNGC 4 cut(s) 684, 1506, 1560, 1584
Sau3AI GATC 5 cut(s) 202, 780, 1327, 1513, 1726
Sau96I GGNCC 7 cut(s) 176, 296, 557, 599, 791, 864, 1688
ScaI AGTACT 2 cut(s) 979, 1274
SchI GAGTC 2 cut(s) 84, 833
ScrFI CCNGG 7 cut(s) 174, 403, 597, 795, 868, 1149, 1611
SfaNI GCATC 2 cut(s) 562, 736
SfoI GGCGCC 1 cut(s) 1267
Sfr274I CTCGAG 1 cut(s) 209
SinI GGWCC 5 cut(s) 296, 557, 599, 791, 1688
SlaI CTCGAG 1 cut(s) 209
SmiMI CAYNNNNRTG 4 cut(s) 258, 1322, 1455, 1702
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SphI GCATGC 2 cut(s) 392, 451
Sse9I AATT 9 cut(s) 36, 482, 511, 672, 901, 1184, 1354, 1401, 1446
SsiI CCGC 7 cut(s) 53, 443, 465, 962, 1224, 1559, 1583
SspDI GGCGCC 1 cut(s) 1265
SspMI CTAG 1 cut(s) 1061
StyD4I CCNGG 7 cut(s) 172, 401, 595, 793, 866, 1147, 1609
StyI CCWWGG 1 cut(s) 1509
TaaI ACNGT 8 cut(s) 66, 157, 296, 358, 643, 733, 1099, 1631
TaiI ACGT 3 cut(s) 662, 813, 1089
TaqI TCGA 6 cut(s) 96, 210, 897, 1101, 1232, 1591
TasI AATT 9 cut(s) 36, 482, 511, 672, 901, 1184, 1354, 1401, 1446
TatI WGTACW 4 cut(s) 753, 977, 1272, 1524
TauI GCSGC 2 cut(s) 1562, 1586
TfiI GAWTC 4 cut(s) 472, 1241, 1588, 1593
Tru1I TTAA 4 cut(s) 102, 216, 1041, 1428
Tru9I TTAA 4 cut(s) 102, 216, 1041, 1428
TscAI CASTG 7 cut(s) 162, 361, 826, 1327, 1438, 1634, 1709
TseFI GTSAC 4 cut(s) 220, 944, 1030, 1631
TseI GCWGC 2 cut(s) 683, 1505
Tsp45I GTSAC 4 cut(s) 220, 944, 1030, 1631
TspGWI ACGGA 2 cut(s) 874, 1068
TspRI CASTG 7 cut(s) 162, 361, 826, 1327, 1438, 1634, 1709
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 5 cut(s) 296, 557, 599, 791, 1688
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 4 cut(s) 482, 511, 1184, 1354
XceI RCATGY 2 cut(s) 392, 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 1575
XhoI CTCGAG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 1101
XspI CTAG 1 cut(s) 1061
ZrmI AGTACT 2 cut(s) 979, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.