pycom14g20210

Monoglyceride lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010185
Physical Location & Seq
Forward (+)
21287703 .. 21291946
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1218 bp
ATGGCGACGGAGAGAGGCAGCGATTTGTCGTCGTCGTCGTTGATGCTGACTTCTGGAGCGAGCGGCCGCATAAACGCCCTCCTCTCGCTGCGGGTGCTGCGGAGTCTGATGATGCTGATTAACGCCTTCTTCCTGCTCCTCCTGCTCCCATTTCGTGGCCGCAAGCGAGCGCCGTCGTCGTCTGCGGCGGCGGCTTCGCCGGTCTTGGCGGAGAAGTCGGCCAAGGACGAGAGGCAGCCTAGGGGGCCGGTGGTCCGGGTGCCAACAACGATTGTTCCGTGGAAGAGCAGCTCGTCGTTGCCGGTGGACACGGAGGTGGCGGCGAGGAGAGCGTTAGCGAAGCTAAGAGTTGTACAAGATGATGATGAGAATTCGGTCCGGCGATATTCGCTCTTTGCGACTGCGAGAGGTGAAACTCTCTTCACGCAAAGTTGGACTCCCGTTTCCGTTAACATCAGGGGCGTAGTCATTCTTATGCATGGTCTGAATGAACACAGTGGAAGATACGACTACTTTGCAAAGCAACTGAATGCTAATGGATTTAAGGTTTACGGAATGGATTGGATTGGTCATGGTGGGAGTGATGGGCTTCATGCATATGTTCCTTCTCTCGATTATGCTGTTACTGATATGAAATTATTTCTCGAAAAGGTTTTAGCTGAAAATCCTGGCCTACCGTGTTTCTGTTTTGGGCATTCCACAGGTGCAGCCATTGTCCTTAAGGTAATGCTTGACCCAAAATTTGAGTCCCGCATAGACGGTGTAGTGCTCACATCACCAGCAGTTGGGGTTCAGCCTGCGCATCCAATATTTGTGGTAATTGCTCCATTGCTCTCATTTTTGCTGCCAAGATACCAAATCAGTGCAGCAAATAAAAAGGGCATGCCTGTCTCTCGTGACCCAGCGGCTCTAGTTGCCAAGTATTCGGACCCTCTAGTGTACACTGGATCCATCCGCGTAAGGACTGGTTATGAGATTCTCCGAATCACATCCTACTTGCAGCAGAACCTAACCAAATTGAGGGTCCCCTTCTTTGTTCTCCACGGCACAGCTGATACTGTTACTGACCCTGAGGCCTCCCAGAAACTGTACGATGAAGGCTCCTCAACTGACAAAACCATCAAACTGTTTGACGGGTTGTTACATGACCTTCTCTTTGAGCCAGAGCGAGAAGCCATTACGAAGGGCATCATTGAGTGGTTGAATCGTAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

406

Amino Acids

44.52

Weight (kDa)

9.42

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 152 - 389 4.3e-77 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 155 - 270 3.1e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 156 - 379 4.4e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 801
AccB1I GGYRCC 1 cut(s) 259
AccB7I CCANNNNNTGG 2 cut(s) 155, 785
AccBSI CCGCTC 1 cut(s) 63
AccII CGCG 1 cut(s) 957
AclWI GGATC 2 cut(s) 942, 955
AcoI YGGCCR 3 cut(s) 64, 157, 219
AcsI RAATTY 2 cut(s) 370, 740
AfaI GTAC 3 cut(s) 354, 941, 1091
AfiI CCNNNNNNNGG 3 cut(s) 155, 785, 1020
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 1 cut(s) 1204
AjnI CCWGG 1 cut(s) 667
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 4 cut(s) 291, 343, 659, 1052
AluI AGCT 4 cut(s) 291, 343, 659, 1052
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 1 cut(s) 895
AlwI GGATC 2 cut(s) 942, 955
AlwNI CAGNNNCTG 1 cut(s) 1087
AoxI GGCC 6 cut(s) 64, 157, 219, 245, 670, 1074
ApeKI GCWGC 9 cut(s) 18, 88, 97, 235, 288, 707, 844, 866, 1000
ApoI RAATTY 2 cut(s) 370, 740
ArsI GACNNNNNNTTYG 8 cut(s) 725, 731, 757, 763, 1008, 1040, 1139, 1171
AspA2I CCTAGG 1 cut(s) 239
AspLEI GCGC 2 cut(s) 172, 802
AspS9I GGNCC 5 cut(s) 245, 253, 376, 928, 1024
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 422, 768
AvaII GGWCC 4 cut(s) 253, 376, 928, 1024
AvrII CCTAGG 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 1071
BamHI GGATCC 1 cut(s) 947
BanI GGYRCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 9 cut(s) 30, 75, 84, 247, 300, 719, 831, 878, 1012
BccI CCATC 3 cut(s) 578, 959, 1127
BceAI ACGGC 2 cut(s) 157, 1060
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 1 cut(s) 669
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 895
BfaI CTAG 3 cut(s) 240, 911, 935
BfoI RGCGCY 1 cut(s) 173
BfrI CTTAAG 1 cut(s) 719
BglI GCCNNNNNGGC 1 cut(s) 244
BlnI CCTAGG 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 257, 669
Bme18I GGWCC 4 cut(s) 253, 376, 928, 1024
BmgT120I GGNCC 5 cut(s) 245, 253, 376, 928, 1024
BmiI GGNNCC 7 cut(s) 246, 261, 930, 949, 1025, 1026, 1102
BmrFI CCNGG 2 cut(s) 257, 669
BmsI GCATC 4 cut(s) 33, 102, 811, 1197
BpmI CTGGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 257
BsaJI CCNNGG 4 cut(s) 222, 239, 278, 1042
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 785, 1020
Bse118I RCCGGY 3 cut(s) 199, 247, 301
Bse1I ACTGG 2 cut(s) 949, 970
Bse21I CCTNAGG 1 cut(s) 1071
Bse3DI GCAATG 1 cut(s) 827
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 4 cut(s) 222, 239, 278, 1042
BseGI GGATG 3 cut(s) 802, 951, 989
BseLI CCNNNNNNNGG 3 cut(s) 155, 785, 1020
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 1 cut(s) 1062
BseNI ACTGG 2 cut(s) 949, 970
BseRI GAGGAG 4 cut(s) 71, 128, 340, 1093
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 9 cut(s) 30, 75, 84, 247, 300, 719, 831, 878, 1012
BseYI CCCAGC 1 cut(s) 901
BsgI GTGCAG 2 cut(s) 726, 885
Bsh1236I CGCG 1 cut(s) 957
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 6 cut(s) 66, 159, 221, 247, 672, 1076
BshNI GGYRCC 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 5 cut(s) 200, 248, 256, 302, 379
BslFI GGGAC 2 cut(s) 733, 1010
BslI CCNNNNNNNGG 3 cut(s) 155, 785, 1020
BsmAI GTCTC 1 cut(s) 895
BsmFI GGGAC 2 cut(s) 733, 1010
BsmI GAATGC 2 cut(s) 535, 694
BsnI GGCC 6 cut(s) 66, 159, 221, 247, 672, 1076
Bsp1286I GDGCHC 1 cut(s) 771
Bsp1407I TGTACA 2 cut(s) 352, 939
Bsp143I GATC 1 cut(s) 947
BspANI GGCC 6 cut(s) 66, 159, 221, 247, 672, 1076
BspCNI CTCAG 1 cut(s) 1063
BspFNI CGCG 1 cut(s) 957
BspLI GGNNCC 7 cut(s) 246, 261, 930, 949, 1025, 1026, 1102
BspPI GGATC 2 cut(s) 942, 955
BspQI GCTCTTC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 719
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 827
BsrFI RCCGGY 3 cut(s) 199, 247, 301
BsrGI TGTACA 2 cut(s) 352, 939
BsrI ACTGG 2 cut(s) 949, 970
BssAI RCCGGY 3 cut(s) 199, 247, 301
BssECI CCNNGG 4 cut(s) 222, 239, 278, 1042
BssMI GATC 1 cut(s) 947
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 2 cut(s) 222, 239
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 6 cut(s) 497, 678, 761, 1060, 1089, 1128
Bst6I CTCTTC 2 cut(s) 278, 425
BstAFI CTTAAG 1 cut(s) 719
BstAUI TGTACA 2 cut(s) 352, 939
BstC8I GCNNGC 5 cut(s) 61, 164, 168, 798, 884
BstDEI CTNAG 2 cut(s) 344, 1071
BstDSI CCRYGG 2 cut(s) 278, 1042
BstF5I GGATG 3 cut(s) 802, 951, 989
BstFNI CGCG 1 cut(s) 957
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 2 cut(s) 172, 802
BstKTI GATC 1 cut(s) 950
BstMAI GTCTC 1 cut(s) 895
BstMBI GATC 1 cut(s) 947
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 8 cut(s) 94, 97, 142, 191, 244, 329, 388, 914
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 1 cut(s) 886
BstSCI CCNGG 2 cut(s) 255, 667
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 9 cut(s) 30, 75, 84, 247, 300, 719, 831, 878, 1012
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
BstZI CGGCCG 1 cut(s) 64
Bsu36I CCTNAGG 1 cut(s) 1071
BsuRI GGCC 6 cut(s) 66, 159, 221, 247, 672, 1076
BtgI CCRYGG 2 cut(s) 278, 1042
BtsCI GGATG 3 cut(s) 802, 951, 989
BtsIMutI CAGTG 3 cut(s) 502, 868, 942
Cac8I GCNNGC 5 cut(s) 61, 164, 168, 798, 884
CaiI CAGNNNCTG 1 cut(s) 1087
CciNI GCGGCCGC 1 cut(s) 64
CfoI GCGC 2 cut(s) 172, 802
Cfr10I RCCGGY 3 cut(s) 199, 247, 301
Cfr13I GGNCC 5 cut(s) 245, 253, 376, 928, 1024
CpoI CGGWCCG 1 cut(s) 376
Csp6I GTAC 3 cut(s) 353, 940, 1090
CspCI CAANNNNNGTGG 2 cut(s) 795, 830
CspI CGGWCCG 1 cut(s) 376
CviAII CATG 5 cut(s) 479, 572, 593, 883, 1145
CviQI GTAC 3 cut(s) 353, 940, 1090
DdeI CTNAG 2 cut(s) 344, 1071
DpnI GATC 1 cut(s) 949
DpnII GATC 1 cut(s) 947
EaeI YGGCCR 3 cut(s) 64, 157, 219
EagI CGGCCG 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 278, 425
EarI CTCTTC 2 cut(s) 278, 425
EciI GGCGGA 1 cut(s) 224
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 2 cut(s) 222, 239
Eco147I AGGCCT 1 cut(s) 1076
Eco47I GGWCC 4 cut(s) 253, 376, 928, 1024
Eco52I CGGCCG 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 1071
EcoO109I RGGNCCY 1 cut(s) 1024
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 222, 239
EcoT22I ATGCAT 2 cut(s) 480, 598
ErhI CCWWGG 2 cut(s) 222, 239
FaeI CATG 5 cut(s) 482, 575, 596, 886, 1148
FaqI GGGAC 2 cut(s) 733, 1010
FatI CATG 5 cut(s) 478, 571, 592, 882, 1144
FauI CCCGC 2 cut(s) 84, 758
FauNDI CATATG 1 cut(s) 598
FokI GGATG 3 cut(s) 789, 938, 976
FspBI CTAG 3 cut(s) 240, 911, 935
FspI TGCGCA 1 cut(s) 801
GlaI GCGC 2 cut(s) 171, 801
GsaI CCCAGC 1 cut(s) 905
GsuI CTGGAG 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 6 cut(s) 66, 159, 221, 247, 672, 1076
HapII CCGG 5 cut(s) 200, 248, 256, 302, 379
HhaI GCGC 2 cut(s) 172, 802
Hin1II CATG 5 cut(s) 482, 575, 596, 886, 1148
Hin6I GCGC 2 cut(s) 170, 800
HinP1I GCGC 2 cut(s) 170, 800
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 6 cut(s) 103, 436, 746, 976, 984, 1204
HpaI GTTAAC 1 cut(s) 451
HpaII CCGG 5 cut(s) 200, 248, 256, 302, 379
HphI GGTGA 2 cut(s) 422, 768
Hpy166II GTNNAC 5 cut(s) 307, 451, 550, 940, 942
Hpy188I TCNGA 4 cut(s) 108, 486, 928, 983
Hpy188III TCNNGA 4 cut(s) 54, 611, 644, 896
Hpy8I GTNNAC 5 cut(s) 307, 451, 550, 940, 942
Hpy99I CGWCG 7 cut(s) 10, 34, 37, 40, 178, 181, 298
HpyAV CCTTC 6 cut(s) 136, 615, 1039, 1091, 1160, 1177
HpyCH4III ACNGT 6 cut(s) 497, 678, 761, 1060, 1089, 1128
HpyCH4V TGCA 6 cut(s) 478, 518, 596, 707, 866, 1000
HpyF10VI GCNNNNNNNGC 8 cut(s) 94, 97, 142, 191, 244, 329, 388, 914
HpyF3I CTNAG 2 cut(s) 344, 1071
Hsp92II CATG 5 cut(s) 482, 575, 596, 886, 1148
HspAI GCGC 2 cut(s) 170, 800
KflI GGGWCCC 1 cut(s) 1024
KspAI GTTAAC 1 cut(s) 451
Kzo9I GATC 1 cut(s) 947
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 5 cut(s) 56, 141, 150, 829, 1106
Lsp1109I GCAGC 9 cut(s) 30, 75, 84, 247, 300, 719, 831, 878, 1012
LweI GCATC 4 cut(s) 33, 102, 811, 1197
MaeI CTAG 3 cut(s) 240, 911, 935
MaeIII GTNAC 4 cut(s) 622, 896, 1060, 1140
MalI GATC 1 cut(s) 949
MbiI CCGCTC 1 cut(s) 63
MboI GATC 1 cut(s) 947
MboII GAAGA 4 cut(s) 121, 295, 412, 513
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 5 cut(s) 370, 635, 740, 819, 1016
MlyI GAGTC 3 cut(s) 112, 430, 755
MmeI TCCRAC 1 cut(s) 413
Mph1103I ATGCAT 2 cut(s) 480, 598
MseI TTAA 4 cut(s) 120, 450, 543, 720
MslI CAYNNNNRTG 3 cut(s) 314, 473, 597
MspA1I CMGCKG 2 cut(s) 905, 1052
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 5 cut(s) 200, 248, 256, 302, 379
MspR9I CCNGG 2 cut(s) 257, 669
Mva1269I GAATGC 2 cut(s) 535, 694
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 8 cut(s) 94, 97, 142, 191, 244, 329, 388, 914
NciI CCSGG 1 cut(s) 257
NdeI CATATG 1 cut(s) 598
NdeII GATC 1 cut(s) 947
NlaIII CATG 5 cut(s) 482, 575, 596, 886, 1148
NlaIV GGNNCC 7 cut(s) 246, 261, 930, 949, 1025, 1026, 1102
NmuCI GTSAC 1 cut(s) 896
NotI GCGGCCGC 1 cut(s) 64
NsbI TGCGCA 1 cut(s) 801
NsiI ATGCAT 2 cut(s) 480, 598
NspI RCATGY 1 cut(s) 886
OliI CACNNNNGTG 1 cut(s) 314
PaeI GCATGC 1 cut(s) 886
PceI AGGCCT 1 cut(s) 1076
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 3 cut(s) 35, 275, 395
PctI GAATGC 2 cut(s) 535, 694
PfeI GAWTC 3 cut(s) 976, 984, 1204
PflMI CCANNNNNTGG 2 cut(s) 155, 785
PleI GAGTC 3 cut(s) 111, 430, 754
PpsI GAGTC 3 cut(s) 111, 430, 754
PpuMI RGGWCCY 1 cut(s) 1024
Psp5II RGGWCCY 1 cut(s) 1024
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 901
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 7 cut(s) 246, 261, 930, 949, 1025, 1026, 1102
PspPI GGNCC 5 cut(s) 245, 253, 376, 928, 1024
PspPPI RGGWCCY 1 cut(s) 1024
PstNI CAGNNNCTG 1 cut(s) 1087
PsuI RGATCY 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 3 cut(s) 354, 941, 1091
RsaNI GTAC 3 cut(s) 353, 940, 1090
RseI CAYNNNNRTG 3 cut(s) 314, 473, 597
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 4 cut(s) 120, 450, 543, 720
Sau3AI GATC 1 cut(s) 947
Sau96I GGNCC 5 cut(s) 245, 253, 376, 928, 1024
SchI GAGTC 3 cut(s) 112, 430, 755
ScrFI CCNGG 2 cut(s) 257, 669
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 4 cut(s) 33, 102, 811, 1197
SinI GGWCC 4 cut(s) 253, 376, 928, 1024
SmiMI CAYNNNNRTG 3 cut(s) 314, 473, 597
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
SphI GCATGC 1 cut(s) 886
Sse9I AATT 5 cut(s) 370, 635, 740, 819, 1016
SseBI AGGCCT 1 cut(s) 1076
SspI AATATT 1 cut(s) 810
SspMI CTAG 3 cut(s) 240, 911, 935
StuI AGGCCT 1 cut(s) 1076
StyD4I CCNGG 2 cut(s) 255, 667
StyI CCWWGG 2 cut(s) 222, 239
TaaI ACNGT 6 cut(s) 497, 678, 761, 1060, 1089, 1128
TaqI TCGA 2 cut(s) 612, 645
TaqII GACCGA 1 cut(s) 364
TasI AATT 5 cut(s) 370, 635, 740, 819, 1016
TatI WGTACW 2 cut(s) 352, 939
TauI GCSGC 8 cut(s) 66, 69, 162, 188, 191, 194, 323, 908
TfiI GAWTC 3 cut(s) 976, 984, 1204
Tru1I TTAA 4 cut(s) 120, 450, 543, 720
Tru9I TTAA 4 cut(s) 120, 450, 543, 720
TscAI CASTG 3 cut(s) 502, 868, 949
TseFI GTSAC 1 cut(s) 896
TseI GCWGC 9 cut(s) 18, 88, 97, 235, 288, 707, 844, 866, 1000
Tsp45I GTSAC 1 cut(s) 896
TspDTI ATGAA 4 cut(s) 504, 581, 647, 1110
TspGWI ACGGA 5 cut(s) 23, 267, 326, 436, 567
TspRI CASTG 3 cut(s) 502, 868, 949
Van91I CCANNNNNTGG 2 cut(s) 155, 785
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 4 cut(s) 253, 376, 928, 1024
XapI RAATTY 2 cut(s) 370, 740
XceI RCATGY 1 cut(s) 886
XmaJI CCTAGG 1 cut(s) 239
XspI CTAG 3 cut(s) 240, 911, 935
Zsp2I ATGCAT 2 cut(s) 480, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.