pycom14g20480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015979
Physical Location & Seq
Forward (+)
21534315 .. 21535308
994 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGTACAATTTGAGTCTTCCTATCATTACACAAATCGTCGGCTATCTAGCCTCCAACTTGAATCAATTTTTAGAAGGTAGAAGGGGCGGAGCGCACGGCAAAGTTGAATATGGTAGTTACCAGGAGACCGGCTGTGCTGTACCACTACCCTTGTCCGGACGGCGCCTTTGCTGCTTTGGCAGCGCACCTCTACTTCTCCGCCGTCTCTACCACCTCGACTTCGTGGGTCCCCCCGGTTTTGTCCAGCAGATCTCCTCCGTGGTCCCAAACGTCGTGGTCTTGGACCACCACAAGACTGCCATTGGAACCCCCATTGGCGGAAATGTGACCGGCGTCGTAGACATGAACAGGAGCGGCGCCAGCGCCACCATCGCTTTCGATTACTTCAGGGACAAAATTGACGCCGACAACAAGGCAGCGGTTGCTCGGTTTGACGGAGTGAGGAGGCTGTTCGAGTATATCGAAGATGGTGATCTATGGAGGTGGAAGCTTCCCAACAGTAAAGCTTTCAGTAGTGGTTTCAAAGATTTGAACCTTCAATACGACGTTGGGTTGAACCCATCTTTGTTCCAACAGCTGCTGTCTTTGGATTTGGAGTCTTTGATTAGTGAAGGAATGGCGAGCTTATCGGAAAAGCAGAAATTAATAGATGAAGCTCTCAGTAAGTCTTATGAAATTTCGCTTGGCGGTGGAGCCTTTGGAAATTGCCTGGCTGTCAATGCAGATTCTATCTCCGAGCTGAGGAGTGAACTCGGACATCAATTAGCTACTAAAAGCCGTAGTCTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

28.65

Weight (kDa)

6.21

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53345
fragaria_vesca FvH4_5g18120
malus_domestica MD06G1236700.v1.1
prunus_persica Prupe.5G241200_v2.0.a1 Prupe.5G241200_v2.0.a1
pyrus_communis pycom06g21240 pycom14g20480
rosa_chinensis RchiOBHm_Chr7g0177491
rosa_laevigata RLG00000005533
rosa_multiflora Rmu_sc0028619.1_g000002
rosa_roxburghii Rroxscaffold_3G00275830
rosa_rugosa Rorug06G0406500
rosa_samantha Rh7CG006300 Rh7DG005800
rosa_wichuraiana Rw7G000490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 162, 356
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 339
AccIII TCCGGA 1 cut(s) 155
AciI CCGC 6 cut(s) 87, 199, 318, 354, 419, 687
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 370
AcyI GRCGYC 4 cut(s) 163, 333, 357, 402
AfaI GTAC 2 cut(s) 5, 141
AfiI CCNNNNNNNGG 3 cut(s) 155, 317, 741
AgsI TTSAA 6 cut(s) 61, 107, 523, 532, 539, 556
AjnI CCWGG 2 cut(s) 120, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 7 cut(s) 490, 506, 577, 624, 656, 739, 767
AluI AGCT 7 cut(s) 490, 506, 577, 624, 656, 739, 767
Alw26I GTCTC 2 cut(s) 119, 209
AlwNI CAGNNNCTG 1 cut(s) 580
Aor13HI TCCGGA 1 cut(s) 155
ApeKI GCWGC 4 cut(s) 171, 180, 416, 577
ApoI RAATTY 1 cut(s) 675
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
AseI ATTAAT 1 cut(s) 644
AspLEI GCGC 5 cut(s) 94, 165, 185, 359, 365
AspS9I GGNCC 3 cut(s) 227, 262, 283
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 3 cut(s) 227, 262, 283
BanI GGYRCC 2 cut(s) 162, 356
BbsI GAAGAC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 740
BbvI GCAGC 4 cut(s) 158, 192, 428, 564
BccI CCATC 3 cut(s) 377, 461, 568
BceAI ACGGC 4 cut(s) 112, 176, 186, 762
BciT130I CCWGG 2 cut(s) 122, 710
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 119, 209
BfaI CTAG 1 cut(s) 47
BfoI RGCGCY 3 cut(s) 166, 360, 366
BglII AGATCT 1 cut(s) 249
BisI GCNGC 5 cut(s) 172, 181, 355, 417, 578
BlsI GCNGC 5 cut(s) 173, 182, 356, 418, 579
Bme1390I CCNGG 3 cut(s) 122, 234, 710
Bme18I GGWCC 3 cut(s) 227, 262, 283
BmgT120I GGNCC 3 cut(s) 227, 262, 283
BmiI GGNNCC 7 cut(s) 164, 228, 229, 264, 307, 358, 694
BmrFI CCNGG 3 cut(s) 122, 234, 710
BoxI GACNNNNGTC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 740
BpuMI CCSGG 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 4 cut(s) 163, 333, 357, 402
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 232, 258
BsaWI WCCGGW 1 cut(s) 155
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 317, 741
Bse118I RCCGGY 2 cut(s) 128, 329
Bse8I GATNNNNATC 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 155
BseBI CCWGG 2 cut(s) 122, 710
BseDI CCNNGG 2 cut(s) 232, 258
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 3 cut(s) 155, 317, 741
BseMII CTCAG 2 cut(s) 673, 731
BseRI GAGGAG 3 cut(s) 244, 457, 757
BseXI GCAGC 4 cut(s) 158, 192, 428, 564
BshNI GGYRCC 2 cut(s) 162, 356
BsiSI CCGG 4 cut(s) 129, 156, 234, 330
BslFI GGGAC 3 cut(s) 213, 248, 404
BslI CCNNNNNNNGG 3 cut(s) 155, 317, 741
BsmAI GTCTC 2 cut(s) 119, 209
BsmBI CGTCTC 1 cut(s) 209
BsmFI GGGAC 3 cut(s) 213, 248, 404
Bso31I GGTCTC 1 cut(s) 119
Bsp13I TCCGGA 1 cut(s) 155
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 249, 472
BspACI CCGC 6 cut(s) 87, 199, 318, 354, 419, 687
BspCNI CTCAG 2 cut(s) 672, 732
BspEI TCCGGA 1 cut(s) 155
BspLI GGNNCC 7 cut(s) 164, 228, 229, 264, 307, 358, 694
BspT107I GGYRCC 2 cut(s) 162, 356
BspTNI GGTCTC 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 2 cut(s) 128, 329
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 2 cut(s) 128, 329
BssECI CCNNGG 2 cut(s) 232, 258
BssMI GATC 2 cut(s) 249, 472
BssNI GRCGYC 4 cut(s) 163, 333, 357, 402
Bst2UI CCWGG 2 cut(s) 122, 710
Bst4CI ACNGT 1 cut(s) 500
BstACI GRCGYC 4 cut(s) 163, 333, 357, 402
BstAPI GCANNNNNTGC 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 361, 622
BstDEI CTNAG 2 cut(s) 659, 740
BstDSI CCRYGG 1 cut(s) 258
BstH2I RGCGCY 3 cut(s) 166, 360, 366
BstHHI GCGC 5 cut(s) 94, 165, 185, 359, 365
BstKTI GATC 2 cut(s) 252, 475
BstMAI GTCTC 2 cut(s) 119, 209
BstMBI GATC 2 cut(s) 249, 472
BstMWI GCNNNNNNNGC 7 cut(s) 171, 177, 180, 360, 371, 422, 719
BstNI CCWGG 2 cut(s) 122, 710
BstPAI GACNNNNGTC 1 cut(s) 332
BstSCI CCNGG 3 cut(s) 120, 232, 708
BstV1I GCAGC 4 cut(s) 158, 192, 428, 564
BstV2I GAAGAC 1 cut(s) 8
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 361, 622
CaiI CAGNNNCTG 1 cut(s) 580
CfoI GCGC 5 cut(s) 94, 165, 185, 359, 365
Cfr10I RCCGGY 2 cut(s) 128, 329
Cfr13I GGNCC 3 cut(s) 227, 262, 283
CseI GACGC 2 cut(s) 322, 410
Csp6I GTAC 2 cut(s) 4, 140
CspCI CAANNNNNGTGG 4 cut(s) 132, 167, 255, 290
CviAII CATG 1 cut(s) 343
CviQI GTAC 2 cut(s) 4, 140
DdeI CTNAG 2 cut(s) 659, 740
DinI GGCGCC 2 cut(s) 164, 358
DpnI GATC 2 cut(s) 251, 474
DpnII GATC 2 cut(s) 249, 472
EciI GGCGGA 3 cut(s) 102, 188, 333
Eco31I GGTCTC 1 cut(s) 119
Eco47I GGWCC 3 cut(s) 227, 262, 283
Eco57I CTGAAG 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 120, 708
EgeI GGCGCC 2 cut(s) 164, 358
EheI GGCGCC 2 cut(s) 164, 358
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 1 cut(s) 346
FaiI YATR 5 cut(s) 111, 344, 459, 478, 672
FaqI GGGAC 3 cut(s) 213, 248, 404
FatI CATG 1 cut(s) 342
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 5 cut(s) 172, 181, 355, 417, 578
Fsp4HI GCNGC 5 cut(s) 172, 181, 355, 417, 578
FspBI CTAG 1 cut(s) 47
GlaI GCGC 5 cut(s) 93, 164, 184, 358, 364
GluI GCNGC 5 cut(s) 172, 181, 355, 417, 578
HaeII RGCGCY 3 cut(s) 166, 360, 366
HapII CCGG 4 cut(s) 129, 156, 234, 330
HgaI GACGC 2 cut(s) 322, 410
HhaI GCGC 5 cut(s) 94, 165, 185, 359, 365
Hin1I GRCGYC 4 cut(s) 163, 333, 357, 402
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 5 cut(s) 92, 163, 183, 357, 363
HinP1I GCGC 5 cut(s) 92, 163, 183, 357, 363
HindIII AAGCTT 2 cut(s) 488, 504
HinfI GANTC 4 cut(s) 13, 61, 596, 725
HpaII CCGG 4 cut(s) 129, 156, 234, 330
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 2 cut(s) 340, 749
Hpy188I TCNGA 3 cut(s) 631, 736, 755
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 2 cut(s) 340, 749
Hpy99I CGWCG 4 cut(s) 41, 275, 338, 548
HpyAV CCTTC 4 cut(s) 68, 75, 545, 605
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 2 cut(s) 270, 546
HpyCH4V TGCA 1 cut(s) 722
HpyF10VI GCNNNNNNNGC 7 cut(s) 171, 177, 180, 360, 371, 422, 719
HpyF3I CTNAG 2 cut(s) 659, 740
HpySE526I ACGT 2 cut(s) 270, 546
Hsp92I GRCGYC 4 cut(s) 163, 333, 357, 402
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 5 cut(s) 92, 163, 183, 357, 363
KasI GGCGCC 2 cut(s) 162, 356
KflI GGGWCCC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 155
Kzo9I GATC 2 cut(s) 249, 472
LmnI GCTCC 3 cut(s) 89, 351, 692
Lsp1109I GCAGC 4 cut(s) 158, 192, 428, 564
MaeI CTAG 1 cut(s) 47
MaeII ACGT 2 cut(s) 270, 546
MaeIII GTNAC 2 cut(s) 116, 325
MalI GATC 2 cut(s) 251, 474
MbiI CCGCTC 1 cut(s) 354
MboI GATC 2 cut(s) 249, 472
MboII GAAGA 2 cut(s) 8, 476
MflI RGATCY 1 cut(s) 249
MluCI AATT 7 cut(s) 7, 65, 396, 641, 675, 703, 761
Mly113I GGCGCC 2 cut(s) 163, 357
MlyI GAGTC 2 cut(s) 22, 605
MmeI TCCRAC 2 cut(s) 78, 595
MnlI CCTC 8 cut(s) 61, 198, 224, 265, 435, 438, 474, 735
MroI TCCGGA 1 cut(s) 155
MseI TTAA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 419, 577
MspI CCGG 4 cut(s) 129, 156, 234, 330
MspR9I CCNGG 3 cut(s) 122, 234, 710
MvaI CCWGG 2 cut(s) 122, 710
MwoI GCNNNNNNNGC 7 cut(s) 171, 177, 180, 360, 371, 422, 719
NarI GGCGCC 2 cut(s) 163, 357
NciI CCSGG 1 cut(s) 234
NdeII GATC 2 cut(s) 249, 472
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 7 cut(s) 164, 228, 229, 264, 307, 358, 694
NmuCI GTSAC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PfeI GAWTC 2 cut(s) 61, 725
PkrI GCNGC 5 cut(s) 173, 182, 356, 418, 579
PleI GAGTC 2 cut(s) 21, 604
PluTI GGCGCC 2 cut(s) 166, 360
PpsI GAGTC 2 cut(s) 21, 604
PpuMI RGGWCCY 1 cut(s) 227
PshAI GACNNNNGTC 1 cut(s) 332
PshBI ATTAAT 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 120, 708
PspGI CCWGG 2 cut(s) 120, 708
PspN4I GGNNCC 7 cut(s) 164, 228, 229, 264, 307, 358, 694
PspPI GGNCC 3 cut(s) 227, 262, 283
PspPPI RGGWCCY 1 cut(s) 227
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
PstNI CAGNNNCTG 1 cut(s) 580
PsuI RGATCY 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 2 cut(s) 5, 141
RsaNI GTAC 2 cut(s) 4, 140
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 5 cut(s) 172, 181, 355, 417, 578
Sau3AI GATC 2 cut(s) 249, 472
Sau96I GGNCC 3 cut(s) 227, 262, 283
SchI GAGTC 2 cut(s) 22, 605
ScrFI CCNGG 3 cut(s) 122, 234, 710
SfoI GGCGCC 2 cut(s) 164, 358
SinI GGWCC 3 cut(s) 227, 262, 283
Sse9I AATT 7 cut(s) 7, 65, 396, 641, 675, 703, 761
SsiI CCGC 6 cut(s) 87, 199, 318, 354, 419, 687
SspDI GGCGCC 2 cut(s) 162, 356
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 120, 232, 708
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 2 cut(s) 273, 549
TaqI TCGA 4 cut(s) 216, 378, 453, 462
TasI AATT 7 cut(s) 7, 65, 396, 641, 675, 703, 761
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 2 cut(s) 61, 725
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 4 cut(s) 171, 180, 416, 577
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 359, 666, 687
TspGWI ACGGA 2 cut(s) 247, 450
VpaK11BI GGWCC 3 cut(s) 227, 262, 283
VspI ATTAAT 1 cut(s) 644
XapI RAATTY 1 cut(s) 675
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.