Rorug06G0406500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015979
Physical Location & Seq
Reverse (-)
55000378 .. 55001666
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0406500.1

Sequence Viewer

Length: 1110 bp
ATGGAGAACGATGACGACTACAGAACCGCAGATAGTGGCGATGAAGAATCACTGAGCTACAGCGCGGGCGATGATGGCTATCTTCCAGAAGATGAACAAGTCGATGACTTTGGTGAGAGTCGATGTCTTTATATTCTTCTGAAAGAAGAAGAAATTGCAAAACTTGAGCAGGATTCTATTTCAGAAGTGTCTACCGTCCTTTCCATATCCAAATCTGATGCATGCTTACTACTCCCTCACTTTCATTGGAGTGTTGAGGATCTACAGGATGAATGGTTTGCTGATGAAGATAAAGTCCGAGACAAGGCAGGCTTGTTGAAGAAACCAATAGTCGAACATAATGCTAGCCAAAATCTAACAACTACTTGCGAAATATGCTTCGAGGAGTACTACTACTATTCATTAGATGCATCATCCAGGAGTAATAGTATTCTCACAGCTTCTTGTGGACATCCTTATTGTCGAGATTGCTTGGCAGGTTATATAAGCACGTCAATCGGTGAGGGTGGTCCTGCCTGTTTGATGCTGAGCCGCTCAGGTGTCCTCAACCGAATGCTGCACTACACGAAGATCAAATGGTGTCCTGCTCCTAATTGCGATTACGCTATTCAATTTGATGCCCATGTTGGAATGGGGTCATGTGATGTCTCTTGTCTTTGTTCACATAGTTATTGCTGGAATTGCACCGAGGAGAATCATAGTCCGGTGGACTGCGAGACGGTGGCGAAGTGGATTTTGAAGAACAAGGATGATTCTCAAAATGCACATTGGATACTGATGTGGGCAGCACTGCAGCAGTTCTGCTGGTCATGCCTCGGGCCTTTGTATGGAGGCTGCAATTGCAATAAATACAGGTCATCTGGGACAGGGATGAGTGCTGTCGAGAGCCAAAAGAAGAAGGCGAAGATGTATTTAGAGAGATACCTTCATTATTTTGAACGCTGGGACAACAATGGAAAATCAAAGCAAAAGGCTCTTGAGGATTTTGATAAGGTGAAGAATGTGCACATACATGAACTTGGAAAGATACAGGGAAATACAAAAGAGCATGAATACAATTTCATCATAGATACTGCGATGGACCTATGCTTATGGCTACTACATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

369

Amino Acids

41.97

Weight (kDa)

4.87

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARIH2_N PF26000 51 - 89 6.2e-06 E3 ubiquitin-protein ligase ARIH2, N-terminal UBA domain
UBA_ARI1 PF21235 57 - 93 5.6e-11 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
zf-RING_UBOX PF13445 123 - 161 8.8e-06 RING-type zinc-finger
IBR PF01485 189 - 238 2.1e-10 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53345
fragaria_vesca FvH4_5g18120
malus_domestica MD06G1236700.v1.1
prunus_persica Prupe.5G241200_v2.0.a1 Prupe.5G241200_v2.0.a1
pyrus_communis pycom06g21240 pycom14g20480
rosa_chinensis RchiOBHm_Chr7g0177491
rosa_laevigata RLG00000005533
rosa_multiflora Rmu_sc0028619.1_g000002
rosa_roxburghii Rroxscaffold_3G00275830
rosa_rugosa Rorug06G0406500
rosa_samantha Rh7CG006300 Rh7DG005800
rosa_wichuraiana Rw7G000490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 534
AccI GTMKAC 1 cut(s) 191
AccII CGCG 1 cut(s) 65
AciI CCGC 3 cut(s) 27, 65, 532
AclWI GGATC 1 cut(s) 267
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 304, 827
AgsI TTSAA 4 cut(s) 319, 611, 739, 938
AjiI CACGTC 1 cut(s) 492
AjnI CCWGG 1 cut(s) 416
AluBI AGCT 2 cut(s) 57, 440
AluI AGCT 2 cut(s) 57, 440
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 3 cut(s) 294, 652, 710
Alw44I GTGCAC 1 cut(s) 1004
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 1 cut(s) 818
ApaLI GTGCAC 1 cut(s) 1004
ApeKI GCWGC 4 cut(s) 556, 785, 793, 834
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 3 cut(s) 509, 818, 1081
AsuHPI GGTGA 3 cut(s) 125, 512, 1006
AsuNHI GCTAGC 1 cut(s) 344
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 2 cut(s) 509, 1081
BaeGI GKGCMC 1 cut(s) 1008
BaeI ACNNNNGTAYC 2 cut(s) 764, 797
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 4 cut(s) 543, 797, 805, 821
BccI CCATC 2 cut(s) 68, 1072
BcgI CGANNNNNNTGC 4 cut(s) 323, 357, 478, 512
BciT130I CCWGG 1 cut(s) 418
BciVI GTATCC 1 cut(s) 765
BcoDI GTCTC 3 cut(s) 294, 652, 710
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 4 cut(s) 19, 58, 263, 791
BfuAI ACCTGC 1 cut(s) 467
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 5 cut(s) 532, 557, 786, 794, 835
BlpI GCTNAGC 1 cut(s) 527
BlsI GCNGC 5 cut(s) 533, 558, 787, 795, 836
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 418
Bme18I GGWCC 2 cut(s) 509, 1081
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 492
BmgT120I GGNCC 3 cut(s) 509, 818, 1081
BmrFI CCNGG 1 cut(s) 418
BmsI GCATC 5 cut(s) 208, 397, 419, 513, 607
BmtI GCTAGC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 535
Bpu1102I GCTNAGC 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 185, 998
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 687, 814
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 827
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 2 cut(s) 687, 814
BseGI GGATG 5 cut(s) 274, 413, 451, 754, 876
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 304, 827
BseMII CTCAG 3 cut(s) 44, 518, 549
BseRI GAGGAG 2 cut(s) 398, 704
BseSI GKGCMC 1 cut(s) 1008
BseXI GCAGC 4 cut(s) 543, 797, 805, 821
BseYI CCCAGC 1 cut(s) 942
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 1008
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 1 cut(s) 704
BslFI GGGAC 2 cut(s) 877, 959
BslI CCNNNNNNNGG 2 cut(s) 304, 827
BsmAI GTCTC 3 cut(s) 294, 652, 710
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 2 cut(s) 877, 959
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 1 cut(s) 820
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 1008
Bsp143I GATC 2 cut(s) 259, 570
Bsp1720I GCTNAGC 1 cut(s) 527
BspACI CCGC 3 cut(s) 27, 65, 532
BspANI GGCC 1 cut(s) 820
BspCNI CTCAG 3 cut(s) 45, 519, 548
BspFNI CGCG 1 cut(s) 65
BspMAI CTGCAG 1 cut(s) 795
BspMI ACCTGC 1 cut(s) 467
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 534
BssECI CCNNGG 2 cut(s) 687, 814
BssMI GATC 2 cut(s) 259, 570
Bst2UI CCWGG 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 196, 721
BstC8I GCNNGC 4 cut(s) 67, 223, 310, 346
BstDEI CTNAG 3 cut(s) 53, 527, 535
BstF5I GGATG 5 cut(s) 274, 413, 451, 754, 876
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 2 cut(s) 262, 573
BstMAI GTCTC 3 cut(s) 294, 652, 710
BstMBI GATC 2 cut(s) 259, 570
BstMWI GCNNNNNNNGC 5 cut(s) 75, 375, 681, 810, 840
BstNI CCWGG 1 cut(s) 418
BstNSI RCATGY 2 cut(s) 225, 1106
BstSCI CCNGG 1 cut(s) 416
BstSFI CTRYAG 4 cut(s) 19, 58, 263, 791
BstSLI GKGCMC 1 cut(s) 1008
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 4 cut(s) 543, 797, 805, 821
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 1 cut(s) 820
BtgZI GCGATG 3 cut(s) 54, 84, 1091
BtrI CACGTC 1 cut(s) 492
BtsCI GGATG 5 cut(s) 274, 413, 451, 754, 876
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 2 cut(s) 50, 788
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 4 cut(s) 67, 223, 310, 346
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 509, 818, 1081
Csp6I GTAC 1 cut(s) 388
CviAII CATG 8 cut(s) 222, 623, 639, 810, 1013, 1049, 1103, 1107
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 53, 527, 535
DpnI GATC 2 cut(s) 261, 572
DpnII GATC 2 cut(s) 259, 570
Eco47I GGWCC 2 cut(s) 509, 1081
Eco88I CYCGRG 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 416
EcoT22I ATGCAT 3 cut(s) 223, 412, 1108
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 8 cut(s) 225, 626, 642, 813, 1016, 1052, 1106, 1110
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 877, 959
FatI CATG 8 cut(s) 221, 622, 638, 809, 1012, 1048, 1102, 1106
FauI CCCGC 1 cut(s) 58
FblI GTMKAC 1 cut(s) 191
Fnu4HI GCNGC 5 cut(s) 532, 557, 786, 794, 835
FokI GGATG 5 cut(s) 281, 400, 438, 761, 883
Fsp4HI GCNGC 5 cut(s) 532, 557, 786, 794, 835
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 64
GluI GCNGC 5 cut(s) 532, 557, 786, 794, 835
GsaI CCCAGC 1 cut(s) 946
HaeIII GGCC 1 cut(s) 820
HapII CCGG 1 cut(s) 704
HhaI GCGC 1 cut(s) 65
Hin1II CATG 8 cut(s) 225, 626, 642, 813, 1016, 1052, 1106, 1110
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 5 cut(s) 47, 118, 173, 694, 752
HpaII CCGG 1 cut(s) 704
HphI GGTGA 3 cut(s) 125, 512, 1006
Hpy166II GTNNAC 5 cut(s) 192, 449, 662, 709, 1006
Hpy188I TCNGA 4 cut(s) 141, 184, 217, 299
Hpy188III TCNNGA 4 cut(s) 86, 464, 883, 977
Hpy8I GTNNAC 5 cut(s) 192, 449, 662, 709, 1006
HpyAV CCTTC 2 cut(s) 892, 935
HpyCH4III ACNGT 2 cut(s) 196, 721
HpyCH4IV ACGT 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 375, 681, 810, 840
HpyF3I CTNAG 3 cut(s) 53, 527, 535
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 8 cut(s) 225, 626, 642, 813, 1016, 1052, 1106, 1110
HspAI GCGC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 259, 570
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 4 cut(s) 543, 797, 805, 821
LweI GCATC 5 cut(s) 208, 397, 419, 513, 607
MaeI CTAG 1 cut(s) 345
MaeII ACGT 1 cut(s) 491
MalI GATC 2 cut(s) 261, 572
MbiI CCGCTC 1 cut(s) 534
MboI GATC 2 cut(s) 259, 570
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 1008
MluCI AATT 6 cut(s) 153, 592, 611, 679, 838, 1057
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 9 cut(s) 246, 250, 376, 496, 554, 682, 824, 824, 973
Mph1103I ATGCAT 3 cut(s) 223, 412, 1108
MslI CAYNNNNRTG 2 cut(s) 249, 1011
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 1 cut(s) 418
MunI CAATTG 1 cut(s) 838
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 418
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 5 cut(s) 75, 375, 681, 810, 840
NdeII GATC 2 cut(s) 259, 570
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 8 cut(s) 225, 626, 642, 813, 1016, 1052, 1106, 1110
NsiI ATGCAT 3 cut(s) 223, 412, 1108
NspI RCATGY 2 cut(s) 225, 1106
PaeI GCATGC 1 cut(s) 225
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 4 cut(s) 47, 173, 694, 752
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 5 cut(s) 533, 558, 787, 795, 836
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 1 cut(s) 416
PspPI GGNCC 3 cut(s) 509, 818, 1081
PstI CTGCAG 1 cut(s) 795
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 2 cut(s) 249, 1011
SatI GCNGC 5 cut(s) 532, 557, 786, 794, 835
Sau3AI GATC 2 cut(s) 259, 570
Sau96I GGNCC 3 cut(s) 509, 818, 1081
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 1 cut(s) 1008
SetI ASST 9 cut(s) 59, 442, 481, 494, 541, 857, 927, 996, 1086
SfaNI GCATC 5 cut(s) 208, 397, 419, 513, 607
SfcI CTRYAG 4 cut(s) 19, 58, 263, 791
SinI GGWCC 2 cut(s) 509, 1081
SmiMI CAYNNNNRTG 2 cut(s) 249, 1011
SmlI CTYRAG 2 cut(s) 164, 977
SmoI CTYRAG 2 cut(s) 164, 977
SphI GCATGC 1 cut(s) 225
Sse9I AATT 6 cut(s) 153, 592, 611, 679, 838, 1057
SsiI CCGC 3 cut(s) 27, 65, 532
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 416
TaaI ACNGT 2 cut(s) 196, 721
TaiI ACGT 1 cut(s) 494
TaqI TCGA 6 cut(s) 102, 121, 333, 381, 463, 882
TasI AATT 6 cut(s) 153, 592, 611, 679, 838, 1057
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 534
TfiI GAWTC 4 cut(s) 47, 173, 694, 752
TscAI CASTG 2 cut(s) 57, 795
TseI GCWGC 4 cut(s) 556, 785, 793, 834
TspRI CASTG 2 cut(s) 57, 795
VneI GTGCAC 1 cut(s) 1004
VpaK11BI GGWCC 2 cut(s) 509, 1081
XceI RCATGY 2 cut(s) 225, 1106
XmiI GTMKAC 1 cut(s) 191
XspI CTAG 1 cut(s) 345
ZrmI AGTACT 1 cut(s) 389
Zsp2I ATGCAT 3 cut(s) 223, 412, 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.