RLG00000005533

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015979
Physical Location & Seq
Forward (+)
68354685 .. 68356933
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005533

Sequence Viewer

Length: 975 bp
ATGAGAAAACCGGCGGTGCTGTACCACTATCCTTGCCCGGACGGCGCGTTTGCTGCTCTGGCGGCGCACCTCTATTTCTCAGCCAAGTCCATCGAACCCTTGTTCTTCCCTAACGCCGTCTACAGCCCCGTCACGACCCAAAGCCTCCCTCTTTCTGACATCGGCGACCTCTACCTCCTCGACTTCGTGGGCCCACCTGGGTTTGTCCAGGAGGTCTCTGCCGAGGTCCCCAACGTCGTCGTTTTGGACCACCACAAGACCGCGATCGGAACAGTGAGCTGTGAGAACGTGACCAGCGTTCTGGACATGAAGAGGAGCGGGGCCACCATTGCTTTCGACTATTTCAGGGGCAAGATTGACGGCGGCGACACGGCGATGAAGGCGAGGTTTGACGGCGTTAGGAGGCTGTTTGAGTATATTGAGGATGGGGATTTGTGGCGGTGGGGGCTTCCTGATAGTAAAGCTTTCAGTAGCGGATTCAAAGATTTGAACTTGCAGTACGACGTCGGATTGAACCCGGCTCTGTTCCAACAGCTGCTTTCTTTGGACTTGGAGTGTTTGATTAGTCGAGGAGTGGCGAGCTTATCAGAAAAGCATAAATTGATAGACGAAGCTCTGAGTCAGTCTTATGAAATCAAACTTGGAGGTGGAGCGTTTGGTCATTGCCTGGCTGTCAATGCAGATTCTATATCGGAGTTGAGGAGTGAACTCGGACATCAGTTGGCTACTAAAAGCCGCTGTCTAAACTTAAGGGGTATTGGAGCTGTTGTATACAAAGTCCCTGAGCTCGAAAATGACGAATTGCTAAAGATAAGCCTTAGGAGTGTGGACAGTGAAGACACAACTCTCATCTCACAGGAATTTGGAGGCGGTGGACATAGAAACGCCAGTTCTTTTATGTTACGATCCGCAGAATTCGAGCAGTGGAAGGTTGGCCAAGCAATCATTGCTGGCTCTGGAAAATGTACAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

35.28

Weight (kDa)

5.31

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53345
fragaria_vesca FvH4_5g18120
malus_domestica MD06G1236700.v1.1
prunus_persica Prupe.5G241200_v2.0.a1 Prupe.5G241200_v2.0.a1
pyrus_communis pycom06g21240 pycom14g20480
rosa_chinensis RchiOBHm_Chr7g0177491
rosa_laevigata RLG00000005533
rosa_multiflora Rmu_sc0028619.1_g000002
rosa_roxburghii Rroxscaffold_3G00275830
rosa_rugosa Rorug06G0406500
rosa_samantha Rh7CG006300 Rh7DG005800
rosa_wichuraiana Rw7G000490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 507
AccBSI CCGCTC 1 cut(s) 318
AccI GTMKAC 2 cut(s) 120, 771
AccII CGCG 2 cut(s) 47, 263
AclWI GGATC 1 cut(s) 900
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 2 cut(s) 860, 914
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 3 cut(s) 23, 500, 967
AflII CTTAAG 1 cut(s) 748
AgsI TTSAA 3 cut(s) 481, 490, 514
AjnI CCWGG 3 cut(s) 196, 207, 666
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
AluBI AGCT 7 cut(s) 279, 464, 535, 582, 614, 764, 787
AluI AGCT 7 cut(s) 279, 464, 535, 582, 614, 764, 787
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 1 cut(s) 900
AoxI GGCC 3 cut(s) 190, 321, 934
ApaI GGGCCC 1 cut(s) 194
ApeKI GCWGC 2 cut(s) 53, 535
ApoI RAATTY 2 cut(s) 860, 914
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 2 cut(s) 47, 67
AspS9I GGNCC 5 cut(s) 190, 191, 226, 247, 321
AsuC2I CCSGG 2 cut(s) 38, 518
AvaII GGWCC 2 cut(s) 226, 247
AxyI CCTNAGG 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 194
BalI TGGCCA 1 cut(s) 936
BanII GRGCYC 2 cut(s) 194, 789
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 2 cut(s) 40, 522
BccI CCATC 2 cut(s) 98, 419
BceAI ACGGC 5 cut(s) 58, 101, 376, 387, 409
BciT130I CCWGG 3 cut(s) 198, 209, 668
BcnI CCSGG 2 cut(s) 38, 518
BcoDI GTCTC 1 cut(s) 220
BfmI CTRYAG 1 cut(s) 121
BfrI CTTAAG 1 cut(s) 748
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 5 cut(s) 54, 63, 364, 536, 736
BlsI GCNGC 5 cut(s) 55, 64, 365, 537, 737
Bme1390I CCNGG 5 cut(s) 38, 198, 209, 518, 668
Bme18I GGWCC 2 cut(s) 226, 247
BmgT120I GGNCC 5 cut(s) 190, 191, 226, 247, 321
BmiI GGNNCC 3 cut(s) 192, 228, 322
BmrFI CCNGG 5 cut(s) 38, 198, 209, 518, 668
BpiI GAAGAC 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 783
BpuMI CCSGG 2 cut(s) 38, 518
BsaHI GRCGYC 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 197, 222
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 888
Bse21I CCTNAGG 1 cut(s) 818
Bse3DI GCAATG 3 cut(s) 327, 661, 945
BseBI CCWGG 3 cut(s) 198, 209, 668
BseDI CCNNGG 2 cut(s) 197, 222
BseGI GGATG 1 cut(s) 430
BseMI GCAATG 3 cut(s) 327, 661, 945
BseMII CTCAG 3 cut(s) 93, 608, 774
BseNI ACTGG 1 cut(s) 888
BseRI GAGGAG 4 cut(s) 167, 328, 585, 715
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 40, 522
Bsh1236I CGCG 2 cut(s) 47, 263
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 3 cut(s) 192, 323, 936
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 789
BsiSI CCGG 3 cut(s) 11, 38, 518
BslFI GGGAC 2 cut(s) 212, 764
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 2 cut(s) 212, 764
BsnI GGCC 3 cut(s) 192, 323, 936
Bso31I GGTCTC 1 cut(s) 220
Bsp120I GGGCCC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 194, 789
Bsp1407I TGTACA 1 cut(s) 965
Bsp143I GATC 2 cut(s) 264, 905
BspANI GGCC 3 cut(s) 192, 323, 936
BspCNI CTCAG 3 cut(s) 92, 609, 775
BspFNI CGCG 2 cut(s) 47, 263
BspLI GGNNCC 3 cut(s) 192, 228, 322
BspPI GGATC 1 cut(s) 900
BspTI CTTAAG 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 318
BsrDI GCAATG 3 cut(s) 327, 661, 945
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 965
BsrI ACTGG 1 cut(s) 888
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 197, 222
BssMI GATC 2 cut(s) 264, 905
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 504
Bst1107I GTATAC 1 cut(s) 772
Bst2UI CCWGG 3 cut(s) 198, 209, 668
Bst4CI ACNGT 2 cut(s) 274, 833
Bst6I CTCTTC 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 504
BstAFI CTTAAG 1 cut(s) 748
BstAPI GCANNNNNTGC 1 cut(s) 947
BstAUI TGTACA 1 cut(s) 965
BstC8I GCNNGC 2 cut(s) 580, 952
BstDEI CTNAG 4 cut(s) 79, 617, 783, 818
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 2 cut(s) 47, 263
BstHHI GCGC 2 cut(s) 47, 67
BstKTI GATC 2 cut(s) 267, 908
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 264, 905
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 9 cut(s) 42, 53, 59, 62, 329, 380, 445, 677, 947
BstNI CCWGG 3 cut(s) 198, 209, 668
BstSCI CCNGG 5 cut(s) 36, 196, 207, 516, 666
BstSFI CTRYAG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 2 cut(s) 47, 263
BstV1I GCAGC 2 cut(s) 40, 522
BstV2I GAAGAC 1 cut(s) 843
BstXI CCANNNNNNTGG 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 772
Bsu36I CCTNAGG 1 cut(s) 818
BsuRI GGCC 3 cut(s) 192, 323, 936
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 929
BtsIMutI CAGTG 3 cut(s) 279, 838, 929
Cac8I GCNNGC 2 cut(s) 580, 952
CfoI GCGC 2 cut(s) 47, 67
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 5 cut(s) 190, 191, 226, 247, 321
Csp6I GTAC 3 cut(s) 22, 499, 966
CspCI CAANNNNNGTGG 2 cut(s) 14, 49
CviAII CATG 1 cut(s) 307
CviQI GTAC 3 cut(s) 22, 499, 966
DdeI CTNAG 4 cut(s) 79, 617, 783, 818
DpnI GATC 2 cut(s) 266, 907
DpnII GATC 2 cut(s) 264, 905
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 787
Eco24I GRGCYC 2 cut(s) 194, 789
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 2 cut(s) 226, 247
Eco53kI GAGCTC 1 cut(s) 787
Eco81I CCTNAGG 1 cut(s) 818
EcoICRI GAGCTC 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 3 cut(s) 196, 207, 666
EcoT38I GRGCYC 2 cut(s) 194, 789
FaeI CATG 1 cut(s) 310
FaiI YATR 8 cut(s) 308, 417, 597, 630, 689, 772, 879, 899
FaqI GGGAC 2 cut(s) 212, 764
FatI CATG 1 cut(s) 306
FauI CCCGC 1 cut(s) 311
FblI GTMKAC 2 cut(s) 120, 771
Fnu4HI GCNGC 5 cut(s) 54, 63, 364, 536, 736
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 2 cut(s) 194, 789
Fsp4HI GCNGC 5 cut(s) 54, 63, 364, 536, 736
GlaI GCGC 2 cut(s) 46, 66
GluI GCNGC 5 cut(s) 54, 63, 364, 536, 736
HaeIII GGCC 3 cut(s) 192, 323, 936
HapII CCGG 3 cut(s) 11, 38, 518
HhaI GCGC 2 cut(s) 47, 67
Hin1I GRCGYC 1 cut(s) 504
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 2 cut(s) 45, 65
HinP1I GCGC 2 cut(s) 45, 65
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 3 cut(s) 477, 619, 683
HpaII CCGG 3 cut(s) 11, 38, 518
Hpy166II GTNNAC 5 cut(s) 121, 707, 772, 829, 875
Hpy188I TCNGA 7 cut(s) 157, 269, 509, 589, 618, 694, 713
Hpy188III TCNNGA 4 cut(s) 133, 302, 452, 957
Hpy8I GTNNAC 5 cut(s) 121, 707, 772, 829, 875
Hpy99I CGWCG 4 cut(s) 239, 242, 506, 509
HpyAV CCTTC 2 cut(s) 373, 922
HpyCH4III ACNGT 2 cut(s) 274, 833
HpyCH4IV ACGT 3 cut(s) 234, 288, 504
HpyCH4V TGCA 2 cut(s) 496, 680
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 53, 59, 62, 329, 380, 445, 677, 947
HpyF3I CTNAG 4 cut(s) 79, 617, 783, 818
HpySE526I ACGT 3 cut(s) 234, 288, 504
Hsp92I GRCGYC 1 cut(s) 504
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 2 cut(s) 45, 65
Kzo9I GATC 2 cut(s) 264, 905
LmnI GCTCC 3 cut(s) 315, 650, 761
Lsp1109I GCAGC 2 cut(s) 40, 522
MaeII ACGT 3 cut(s) 234, 288, 504
MaeIII GTNAC 3 cut(s) 130, 289, 900
MalI GATC 2 cut(s) 266, 907
MbiI CCGCTC 1 cut(s) 318
MboI GATC 2 cut(s) 264, 905
MboII GAAGA 3 cut(s) 97, 322, 848
MhlI GDGCHC 2 cut(s) 194, 789
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 4 cut(s) 599, 800, 860, 914
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 2 cut(s) 487, 553
Mox20I TGGCCA 1 cut(s) 936
MscI TGGCCA 1 cut(s) 936
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 2 cut(s) 535, 738
MspCI CTTAAG 1 cut(s) 748
MspI CCGG 3 cut(s) 11, 38, 518
MspR9I CCNGG 5 cut(s) 38, 198, 209, 518, 668
MvaI CCWGG 3 cut(s) 198, 209, 668
MvnI CGCG 2 cut(s) 47, 263
MwoI GCNNNNNNNGC 9 cut(s) 42, 53, 59, 62, 329, 380, 445, 677, 947
NciI CCSGG 2 cut(s) 38, 518
NdeII GATC 2 cut(s) 264, 905
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 3 cut(s) 192, 228, 322
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 130, 289
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 795
PfeI GAWTC 2 cut(s) 477, 683
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 5 cut(s) 55, 64, 365, 537, 737
Ple19I CGATCG 1 cut(s) 267
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
PpuMI RGGWCCY 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 3 cut(s) 196, 207, 666
PspGI CCWGG 3 cut(s) 196, 207, 666
PspN4I GGNNCC 3 cut(s) 192, 228, 322
PspOMI GGGCCC 1 cut(s) 190
PspPI GGNCC 5 cut(s) 190, 191, 226, 247, 321
PspPPI RGGWCCY 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 482, 514
PvuI CGATCG 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 3 cut(s) 23, 500, 967
RsaNI GTAC 3 cut(s) 22, 499, 966
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 789
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 5 cut(s) 54, 63, 364, 536, 736
Sau3AI GATC 2 cut(s) 264, 905
Sau96I GGNCC 5 cut(s) 190, 191, 226, 247, 321
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 5 cut(s) 38, 198, 209, 518, 668
SduI GDGCHC 2 cut(s) 194, 789
SfcI CTRYAG 1 cut(s) 121
SinI GGWCC 2 cut(s) 226, 247
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
Sse9I AATT 4 cut(s) 599, 800, 860, 914
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 5 cut(s) 36, 196, 207, 516, 666
TaaI ACNGT 2 cut(s) 274, 833
TaiI ACGT 3 cut(s) 237, 291, 507
TaqI TCGA 6 cut(s) 93, 180, 336, 568, 789, 918
TasI AATT 4 cut(s) 599, 800, 860, 914
TatI WGTACW 1 cut(s) 965
TauI GCSGC 3 cut(s) 65, 366, 738
TfiI GAWTC 2 cut(s) 477, 683
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 3 cut(s) 279, 838, 929
TseFI GTSAC 2 cut(s) 130, 289
TseI GCWGC 2 cut(s) 53, 535
Tsp45I GTSAC 2 cut(s) 130, 289
TspDTI ATGAA 3 cut(s) 323, 392, 645
TspRI CASTG 3 cut(s) 279, 838, 929
Vha464I CTTAAG 1 cut(s) 748
VpaK11BI GGWCC 2 cut(s) 226, 247
XapI RAATTY 2 cut(s) 860, 914
XmiI GTMKAC 2 cut(s) 120, 771
ZraI GACGTC 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.