pycom15g00140

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009884
Physical Location & Seq
Forward (+)
81322 .. 85799
4478 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1737 bp
ATGGCTTTTCATTTCCAGCGCCGCCGCCTCCACTACTACCACCGCTTTCGCTTTCTTCTTCCCCTCATTTCCGCCATATCCGGAGCCCTTCTCGTCCTCTTCGCTCTTCTCTTGGTTCTTGCTCCTTCCCCTCTCGATGTCAATAACCTCTATCAACGGCGTCATTCTTCTACGTTCGATGTTGCCGTGGACGACGATGCCGTTGCTGCACGGCTTACTGTCTTTCGTGTTCCAAGTGGTGGAGGGAGATTGGATCGTGATATTTGGAGCTCCAGGAATTCCAAATTCTACTATGGATGCAGCAATGGCACCAAGAAATTTCCAAATGTTGATGAAATTACGCGTCCTAATAGGTACTTGGCGATTGCAACCAGTGGAGGCTTGAATCAACAAAGAACTGGGATAACAGATGCCGTTGTAGCAGCTCGTATACTGAATGCTAGTCTTGTTGTTCCAAAGTTGGACCAGAAATCTTTCTGGAAGGATGCAAGTAACTTTTCAGAGATTTTTGATGTTGATTGGTTTATATCATTTCTGTCAAAAGATGTTAAAATCATAAAACAGCTTCCAAGAAAGGGAGGTAACATGTGGACGCCATCCACTATGCGTGTTCCAAGGAAGTGTAGTGAAAGATGTTATCAAAATCGTGTATTACCTGTTCTTTTGAAAAGGCATGCTATTCAGCTCAACAAGTTTGATTACAGACTTTCAAACAGGCTGAATACAGATCTGCAAAAATTAAGATGTAGAGTCAACTACCATGCTTTAAAGTTTACTGACCCAATACAGAAAATGGCTAAAAAGTTGGTTCAACAAATGAGGTTGAGAAGCAAGCATTACATCGCATTGCACCTGAGGTATGAACCTGATATGCTTGCATTCTCAGGATGTTATTATGGTGGAGGAGACAAGGAAAGGAAAGAATTGGGTGCCATACGAAAGAGGTGGAAGACTTTACATATTAGCAATCCTGATAAGCCAAGGAGGCATGGGAGGTGTCCACTTACTCCAGAAGAAGTTGGTCTTATGTTGAGAGCACTGGGTTATGGCAGCGATGTTCACATCTATGTGGCATCAGGGGAAGTCTATGGAGGAGAAGAAACATTGGCACCCCTTAAGGCACTATTCCCCAACTTTTATTCGAAAGAGACAATAGCACCCAAGGAGGAATTGGAACCATTTTATTCATTTTCTTCTCGCATGGCGGCACTTGACTTCATAGTTTGTGATGAGAGTGATGTCTTTGTCACCAACAACAATGGCAATATGGCCAAAATATTAGCCGGACGAAGGAGATATTTTGGACATAAGCCCACTATTCGTCCAAATGCTAAAAAACTCTACCGGTTGTTCCTAAGCAAAGAGAACATGACCTGGGGAGCTTTTGCTTCTAATGTTCGTACTTATCAGAGAGGCTTTATGGGTGAGCCGAATGAAGTGAGGCCAGGCAGGGGTGGTTTTCATGAGAACCCATATACCTGCATATGTGAAGATTCTCAGGCCAAGGCGAAGAGGGATGTGGGACCTAGAAAATATGGTAGGAGTAGTAACAAAACAGGAAAAGACGAAGTAGAGTTTGATGCCCAAAACGTGGAAGATGATCCAGAAGGGCCTGATACTGATGAGGATGAATATCAAAGTGCCCCCTTGGACAATGATTTGTTTAACAGAAGAGGCGTGGATTATGACGTTGTCAACTCCGAGGAACCTGAATTGGAGGAGATGCTTTCAGACTAG

Protein Analysis

579

Amino Acids

66.25

Weight (kDa)

9.32

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 119 - 433 8.1e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1487
AccB1I GGYRCC 3 cut(s) 308, 929, 1108
AccB7I CCANNNNNTGG 2 cut(s) 239, 1591
AccI GTMKAC 1 cut(s) 430
AccII CGCG 1 cut(s) 343
AccIII TCCGGA 1 cut(s) 80
AciI CCGC 5 cut(s) 22, 25, 43, 72, 1205
AclWI GGATC 2 cut(s) 261, 1595
AcoI YGGCCR 1 cut(s) 1269
AcsI RAATTY 3 cut(s) 277, 284, 317
AcyI GRCGYC 2 cut(s) 160, 593
AfaI GTAC 2 cut(s) 356, 1402
AfiI CCNNNNNNNGG 5 cut(s) 239, 575, 1290, 1451, 1591
AflII CTTAAG 1 cut(s) 1115
AflIII ACRYGT 2 cut(s) 341, 585
AgeI ACCGGT 1 cut(s) 1344
AgsI TTSAA 4 cut(s) 385, 667, 711, 812
AjnI CCWGG 3 cut(s) 272, 1373, 1444
AluBI AGCT 5 cut(s) 270, 425, 565, 685, 1382
AluI AGCT 5 cut(s) 270, 425, 565, 685, 1382
Alw21I GWGCWC 2 cut(s) 272, 1039
Alw26I GTCTC 2 cut(s) 900, 1142
AlwI GGATC 2 cut(s) 261, 1595
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 4 cut(s) 1269, 1442, 1500, 1610
ApeKI GCWGC 4 cut(s) 206, 300, 422, 1050
ApoI RAATTY 3 cut(s) 277, 284, 317
AsiGI ACCGGT 1 cut(s) 1344
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 3 cut(s) 463, 1523, 1610
AsuHPI GGTGA 2 cut(s) 1240, 1436
AsuII TTCGAA 1 cut(s) 1142
AvaII GGWCC 2 cut(s) 463, 1523
AxyI CCTNAGG 1 cut(s) 854
BaeGI GKGCMC 1 cut(s) 1645
BalI TGGCCA 1 cut(s) 1271
BanI GGYRCC 3 cut(s) 308, 929, 1108
BanII GRGCYC 2 cut(s) 88, 272
BbsI GAAGAC 1 cut(s) 956
Bbv12I GWGCWC 2 cut(s) 272, 1039
BbvI GCAGC 4 cut(s) 193, 312, 434, 1062
BccI CCATC 1 cut(s) 604
BceAI ACGGC 5 cut(s) 170, 173, 185, 227, 398
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 3 cut(s) 274, 1375, 1446
BcoDI GTCTC 2 cut(s) 900, 1142
BfaI CTAG 3 cut(s) 441, 1527, 1735
BfoI RGCGCY 1 cut(s) 22
BfrI CTTAAG 1 cut(s) 1115
BfuAI ACCTGC 1 cut(s) 1487
BglI GCCNNNNNGGC 1 cut(s) 985
BglII AGATCT 1 cut(s) 727
BisI GCNGC 7 cut(s) 22, 25, 207, 301, 423, 1051, 1206
BlsI GCNGC 7 cut(s) 23, 26, 208, 302, 424, 1052, 1207
Bme1390I CCNGG 3 cut(s) 274, 1375, 1446
Bme18I GGWCC 2 cut(s) 463, 1523
BmgT120I GGNCC 3 cut(s) 463, 1523, 1610
BmiI GGNNCC 7 cut(s) 85, 310, 931, 1110, 1176, 1524, 1707
BmrFI CCNGG 3 cut(s) 274, 1375, 1446
BmrI ACTGGG 2 cut(s) 408, 1049
BmsI GCATC 7 cut(s) 187, 287, 400, 475, 1082, 1570, 1713
BmuI ACTGGG 2 cut(s) 408, 1049
BpiI GAAGAC 1 cut(s) 956
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 2 cut(s) 256, 993
Bpu10I CCTNAGC 1 cut(s) 1355
Bpu14I TTCGAA 1 cut(s) 1142
BsaBI GATNNNNATC 1 cut(s) 1632
BsaHI GRCGYC 2 cut(s) 160, 593
BsaJI CCNNGG 8 cut(s) 186, 614, 980, 1161, 1374, 1503, 1647, 1701
BsaWI WCCGGW 2 cut(s) 80, 1344
Bsc4I CCNNNNNNNGG 5 cut(s) 239, 575, 1290, 1451, 1591
Bse118I RCCGGY 1 cut(s) 1344
Bse1I ACTGG 3 cut(s) 372, 403, 1044
Bse21I CCTNAGG 1 cut(s) 854
Bse3DI GCAATG 2 cut(s) 310, 845
Bse8I GATNNNNATC 1 cut(s) 1632
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 3 cut(s) 274, 1375, 1446
BseDI CCNNGG 8 cut(s) 186, 614, 980, 1161, 1374, 1503, 1647, 1701
BseGI GGATG 6 cut(s) 302, 490, 596, 893, 1522, 1633
BseJI GATNNNNATC 1 cut(s) 1632
BseLI CCNNNNNNNGG 5 cut(s) 239, 575, 1290, 1451, 1591
BseMI GCAATG 2 cut(s) 310, 845
BseMII CTCAG 3 cut(s) 845, 897, 1511
BseNI ACTGG 3 cut(s) 372, 403, 1044
BseRI GAGGAG 3 cut(s) 918, 1107, 1733
BseSI GKGCMC 1 cut(s) 1645
BseXI GCAGC 4 cut(s) 193, 312, 434, 1062
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 4 cut(s) 1271, 1444, 1502, 1612
BshNI GGYRCC 3 cut(s) 308, 929, 1108
BshTI ACCGGT 1 cut(s) 1344
BsiHKAI GWGCWC 2 cut(s) 272, 1039
BsiSI CCGG 3 cut(s) 81, 1284, 1345
BslFI GGGAC 1 cut(s) 1536
BslI CCNNNNNNNGG 5 cut(s) 239, 575, 1290, 1451, 1591
BsmAI GTCTC 2 cut(s) 900, 1142
BsmFI GGGAC 1 cut(s) 1536
BsmI GAATGC 2 cut(s) 442, 878
BsnI GGCC 4 cut(s) 1271, 1444, 1502, 1612
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 4 cut(s) 88, 272, 1039, 1645
Bsp13I TCCGGA 1 cut(s) 80
Bsp143I GATC 3 cut(s) 253, 727, 1600
BspACI CCGC 5 cut(s) 22, 25, 43, 72, 1205
BspANI GGCC 4 cut(s) 1271, 1444, 1502, 1612
BspCNI CTCAG 3 cut(s) 846, 896, 1510
BspEI TCCGGA 1 cut(s) 80
BspFNI CGCG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 7 cut(s) 85, 310, 931, 1110, 1176, 1524, 1707
BspMI ACCTGC 1 cut(s) 1487
BspPI GGATC 2 cut(s) 261, 1595
BspQI GCTCTTC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 1142
BspT107I GGYRCC 3 cut(s) 308, 929, 1108
BspTI CTTAAG 1 cut(s) 1115
BsrDI GCAATG 2 cut(s) 310, 845
BsrFI RCCGGY 1 cut(s) 1344
BsrI ACTGG 3 cut(s) 372, 403, 1044
BssAI RCCGGY 1 cut(s) 1344
BssECI CCNNGG 8 cut(s) 186, 614, 980, 1161, 1374, 1503, 1647, 1701
BssMI GATC 3 cut(s) 253, 727, 1600
BssNAI GTATAC 1 cut(s) 431
BssNI GRCGYC 2 cut(s) 160, 593
BssT1I CCWWGG 5 cut(s) 614, 980, 1161, 1503, 1647
Bst1107I GTATAC 1 cut(s) 431
Bst2UI CCWGG 3 cut(s) 274, 1375, 1446
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 104, 111, 1505, 1666
BstACI GRCGYC 2 cut(s) 160, 593
BstAFI CTTAAG 1 cut(s) 1115
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 3 cut(s) 675, 833, 876
BstDEI CTNAG 4 cut(s) 854, 883, 1355, 1497
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 6 cut(s) 302, 490, 596, 893, 1522, 1633
BstFNI CGCG 1 cut(s) 343
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 3 cut(s) 256, 730, 1603
BstMAI GTCTC 2 cut(s) 900, 1142
BstMBI GATC 3 cut(s) 253, 727, 1600
BstMWI GCNNNNNNNGC 4 cut(s) 206, 306, 419, 985
BstNI CCWGG 3 cut(s) 274, 1375, 1446
BstNSI RCATGY 2 cut(s) 589, 677
BstSCI CCNGG 3 cut(s) 272, 1373, 1444
BstSLI GKGCMC 1 cut(s) 1645
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 4 cut(s) 193, 312, 434, 1062
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BstZ17I GTATAC 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 854
BsuRI GGCC 4 cut(s) 1271, 1444, 1502, 1612
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 2 cut(s) 826, 1068
BtsCI GGATG 6 cut(s) 302, 490, 596, 893, 1522, 1633
BtsIMutI CAGTG 2 cut(s) 379, 1037
BveI ACCTGC 1 cut(s) 1487
Cac8I GCNNGC 3 cut(s) 675, 833, 876
CciI TCATGA 1 cut(s) 1462
CfoI GCGC 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 1344
Cfr13I GGNCC 3 cut(s) 463, 1523, 1610
CseI GACGC 3 cut(s) 149, 332, 601
Csp6I GTAC 2 cut(s) 355, 1401
CspAI ACCGGT 1 cut(s) 1344
CviAII CATG 7 cut(s) 586, 674, 761, 989, 1201, 1369, 1463
CviQI GTAC 2 cut(s) 355, 1401
DdeI CTNAG 4 cut(s) 854, 883, 1355, 1497
DpnI GATC 3 cut(s) 255, 729, 1602
DpnII GATC 3 cut(s) 253, 727, 1600
DraI TTTAAA 1 cut(s) 768
EaeI YGGCCR 1 cut(s) 1269
Eam1104I CTCTTC 4 cut(s) 104, 111, 1505, 1666
EarI CTCTTC 4 cut(s) 104, 111, 1505, 1666
EciI GGCGGA 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 5 cut(s) 614, 980, 1161, 1503, 1647
Eco24I GRGCYC 2 cut(s) 88, 272
Eco47I GGWCC 2 cut(s) 463, 1523
Eco53kI GAGCTC 1 cut(s) 270
Eco81I CCTNAGG 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 270
EcoO109I RGGNCCY 2 cut(s) 1523, 1610
EcoRI GAATTC 1 cut(s) 277
EcoRII CCWGG 3 cut(s) 272, 1373, 1444
EcoT14I CCWWGG 5 cut(s) 614, 980, 1161, 1503, 1647
EcoT38I GRGCYC 2 cut(s) 88, 272
ErhI CCWWGG 5 cut(s) 614, 980, 1161, 1503, 1647
FaeI CATG 7 cut(s) 589, 677, 764, 992, 1204, 1372, 1466
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FaqI GGGAC 1 cut(s) 1536
FatI CATG 7 cut(s) 585, 673, 760, 988, 1200, 1368, 1462
FauNDI CATATG 1 cut(s) 1484
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 7 cut(s) 22, 25, 207, 301, 423, 1051, 1206
FokI GGATG 6 cut(s) 309, 497, 583, 900, 1529, 1640
FriOI GRGCYC 2 cut(s) 88, 272
Fsp4HI GCNGC 7 cut(s) 22, 25, 207, 301, 423, 1051, 1206
FspBI CTAG 3 cut(s) 441, 1527, 1735
GlaI GCGC 1 cut(s) 20
GluI GCNGC 7 cut(s) 22, 25, 207, 301, 423, 1051, 1206
GsuI CTGGAG 2 cut(s) 256, 993
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 4 cut(s) 1271, 1444, 1502, 1612
HapII CCGG 3 cut(s) 81, 1284, 1345
HgaI GACGC 3 cut(s) 149, 332, 601
HhaI GCGC 1 cut(s) 21
Hin1I GRCGYC 2 cut(s) 160, 593
Hin1II CATG 7 cut(s) 589, 677, 764, 992, 1204, 1372, 1466
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 2 cut(s) 754, 1696
HindII GTYRAC 2 cut(s) 754, 1696
HinfI GANTC 3 cut(s) 385, 750, 1493
HpaII CCGG 3 cut(s) 81, 1284, 1345
HphI GGTGA 2 cut(s) 1240, 1436
Hpy166II GTNNAC 8 cut(s) 190, 431, 591, 754, 774, 1001, 1060, 1696
Hpy188I TCNGA 4 cut(s) 502, 1410, 1702, 1732
Hpy188III TCNNGA 9 cut(s) 81, 134, 257, 478, 885, 971, 1010, 1463, 1604
Hpy8I GTNNAC 8 cut(s) 190, 431, 591, 754, 774, 1001, 1060, 1696
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 5 cut(s) 98, 135, 475, 1284, 1601
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 3 cut(s) 173, 1590, 1689
HpyCH4V TGCA 8 cut(s) 209, 300, 368, 488, 733, 850, 878, 1482
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 306, 419, 985
HpyF3I CTNAG 4 cut(s) 854, 883, 1355, 1497
HpySE526I ACGT 3 cut(s) 173, 1590, 1689
Hsp92I GRCGYC 2 cut(s) 160, 593
Hsp92II CATG 7 cut(s) 589, 677, 764, 992, 1204, 1372, 1466
HspAI GCGC 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 80
Kzo9I GATC 3 cut(s) 253, 727, 1600
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 5 cut(s) 83, 127, 267, 275, 1379
Lsp1109I GCAGC 4 cut(s) 193, 312, 434, 1062
LweI GCATC 7 cut(s) 187, 287, 400, 475, 1082, 1570, 1713
MaeI CTAG 3 cut(s) 441, 1527, 1735
MaeII ACGT 3 cut(s) 173, 1590, 1689
MaeIII GTNAC 4 cut(s) 491, 581, 1246, 1547
MalI GATC 3 cut(s) 255, 729, 1602
MboI GATC 3 cut(s) 253, 727, 1600
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 4 cut(s) 88, 272, 1039, 1645
MlsI TGGCCA 1 cut(s) 1271
MluCI AATT 8 cut(s) 277, 284, 317, 336, 737, 923, 1169, 1712
MluI ACGCGT 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 1271
MlyI GAGTC 1 cut(s) 759
MmeI TCCRAC 1 cut(s) 441
Mox20I TGGCCA 1 cut(s) 1271
MroI TCCGGA 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 473
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 5 cut(s) 549, 740, 767, 1116, 1665
MslI CAYNNNNRTG 2 cut(s) 1065, 1067
Msp20I TGGCCA 1 cut(s) 1271
MspCI CTTAAG 1 cut(s) 1115
MspI CCGG 3 cut(s) 81, 1284, 1345
MspR9I CCNGG 3 cut(s) 274, 1375, 1446
Mva1269I GAATGC 2 cut(s) 442, 878
MvaI CCWGG 3 cut(s) 274, 1375, 1446
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 4 cut(s) 206, 306, 419, 985
NdeI CATATG 1 cut(s) 1484
NdeII GATC 3 cut(s) 253, 727, 1600
NlaIII CATG 7 cut(s) 589, 677, 764, 992, 1204, 1372, 1466
NlaIV GGNNCC 7 cut(s) 85, 310, 931, 1110, 1176, 1524, 1707
NmuCI GTSAC 1 cut(s) 1246
NspI RCATGY 2 cut(s) 589, 677
NspV TTCGAA 1 cut(s) 1142
PaeI GCATGC 1 cut(s) 677
PagI TCATGA 1 cut(s) 1462
PciI ACATGT 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 198
PctI GAATGC 2 cut(s) 442, 878
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 385, 1493
PflFI GACNNNGTC 1 cut(s) 1691
PflMI CCANNNNNTGG 2 cut(s) 239, 1591
PfoI TCCNGGA 1 cut(s) 272
PinAI ACCGGT 1 cut(s) 1344
PkrI GCNGC 7 cut(s) 23, 26, 208, 302, 424, 1052, 1207
PleI GAGTC 1 cut(s) 758
PpsI GAGTC 1 cut(s) 758
PpuMI RGGWCCY 1 cut(s) 1523
PscI ACATGT 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 1523
Psp6I CCWGG 3 cut(s) 272, 1373, 1444
PspGI CCWGG 3 cut(s) 272, 1373, 1444
PspN4I GGNNCC 7 cut(s) 85, 310, 931, 1110, 1176, 1524, 1707
PspPI GGNCC 3 cut(s) 463, 1523, 1610
PspPPI RGGWCCY 1 cut(s) 1523
PsrI GAACNNNNNNTAC 2 cut(s) 642, 674
PsuI RGATCY 1 cut(s) 727
PsyI GACNNNGTC 1 cut(s) 1691
RsaI GTAC 2 cut(s) 356, 1402
RsaNI GTAC 2 cut(s) 355, 1401
RseI CAYNNNNRTG 2 cut(s) 1065, 1067
SacI GAGCTC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 5 cut(s) 549, 740, 767, 1116, 1665
SatI GCNGC 7 cut(s) 22, 25, 207, 301, 423, 1051, 1206
Sau3AI GATC 3 cut(s) 253, 727, 1600
Sau96I GGNCC 3 cut(s) 463, 1523, 1610
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 3 cut(s) 274, 1375, 1446
SduI GDGCHC 4 cut(s) 88, 272, 1039, 1645
SfaNI GCATC 7 cut(s) 187, 287, 400, 475, 1082, 1570, 1713
SfuI TTCGAA 1 cut(s) 1142
SinI GGWCC 2 cut(s) 463, 1523
SmiMI CAYNNNNRTG 2 cut(s) 1065, 1067
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SphI GCATGC 1 cut(s) 677
Sse9I AATT 8 cut(s) 277, 284, 317, 336, 737, 923, 1169, 1712
SsiI CCGC 5 cut(s) 22, 25, 43, 72, 1205
SspI AATATT 1 cut(s) 1278
SspMI CTAG 3 cut(s) 441, 1527, 1735
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 272, 1373, 1444
StyI CCWWGG 5 cut(s) 614, 980, 1161, 1503, 1647
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 3 cut(s) 176, 1593, 1692
TaqI TCGA 3 cut(s) 135, 177, 1142
TasI AATT 8 cut(s) 277, 284, 317, 336, 737, 923, 1169, 1712
TauI GCSGC 3 cut(s) 24, 27, 1208
TfiI GAWTC 2 cut(s) 385, 1493
Tru1I TTAA 5 cut(s) 549, 740, 767, 1116, 1665
Tru9I TTAA 5 cut(s) 549, 740, 767, 1116, 1665
TscAI CASTG 2 cut(s) 379, 1044
TseFI GTSAC 1 cut(s) 1246
TseI GCWGC 4 cut(s) 206, 300, 422, 1050
Tsp45I GTSAC 1 cut(s) 1246
TspDTI ATGAA 7 cut(s) 348, 876, 1176, 1207, 1449, 1451, 1644
TspRI CASTG 2 cut(s) 379, 1044
Tth111I GACNNNGTC 1 cut(s) 1691
Van91I CCANNNNNTGG 2 cut(s) 239, 1591
Vha464I CTTAAG 1 cut(s) 1115
VpaK11BI GGWCC 2 cut(s) 463, 1523
XapI RAATTY 3 cut(s) 277, 284, 317
XceI RCATGY 2 cut(s) 589, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 1168
XmiI GTMKAC 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 3 cut(s) 441, 1527, 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.