pycom15g00540

Belongs to the complex I LYR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009199
Physical Location & Seq
Forward (+)
319671 .. 320319
649 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 321 bp
ATGTACAAATTAAGACCAATTGAAGGCCCGATAACATTGGCCAATCCACAAGGCGACCGCGAACTCCTCGTACAGTCAAGCTCCCGCTACTGCTACTACTCCTACGTGTTGGGGTTATGGCTAAAGCCCTTGATTTGCAGGACTTCCTTATCCGCGGTCGAAGCCCCCGTTGATTCCAGAGGTGAACTAAGATCAATGATTCGACAGGAACTGGAGAACAACAGGCGCTGCAATGACAGGCAACGGACTCGTTTTCTGCTTAGTGAAGGTTTGGAGAGACTGAAACGTCTCGACGACATGCCTGACATGCAAGGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_LYR

Protein Analysis

107

Amino Acids

12.42

Weight (kDa)

8.93

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_LYR PF05347 56 - 95 8.2e-06 Complex 1 protein (LYR family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 285
AccII CGCG 2 cut(s) 60, 155
AciI CCGC 4 cut(s) 58, 85, 153, 155
AcoI YGGCCR 1 cut(s) 39
AfaI GTAC 2 cut(s) 5, 72
AfiI CCNNNNNNNGG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 1 cut(s) 23
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 271, 293
AlwNI CAGNNNCTG 2 cut(s) 211, 228
AoxI GGCC 2 cut(s) 25, 39
ApeKI GCWGC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 194
BalI TGGCCA 1 cut(s) 41
BbvI GCAGC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BcoDI GTCTC 2 cut(s) 271, 293
BfaI CTAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 229
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 2 cut(s) 60, 155
Bsh1285I CGRYCG 2 cut(s) 58, 159
BshFI GGCC 2 cut(s) 27, 41
BsiEI CGRYCG 2 cut(s) 58, 159
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 2 cut(s) 271, 293
BsmBI CGTCTC 1 cut(s) 293
BsnI GGCC 2 cut(s) 27, 41
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 191
BspACI CCGC 4 cut(s) 58, 85, 153, 155
BspANI GGCC 2 cut(s) 27, 41
BspFNI CGCG 2 cut(s) 60, 155
BsrDI GCAATG 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 216
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 106
BstDEI CTNAG 2 cut(s) 188, 260
BstDSI CCRYGG 1 cut(s) 153
BstFNI CGCG 2 cut(s) 60, 155
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 2 cut(s) 271, 293
BstMBI GATC 1 cut(s) 191
BstMCI CGRYCG 2 cut(s) 58, 159
BstMWI GCNNNNNNNGC 2 cut(s) 161, 307
BstNSI RCATGY 2 cut(s) 301, 310
BstUI CGCG 2 cut(s) 60, 155
BstV1I GCAGC 1 cut(s) 215
BsuRI GGCC 2 cut(s) 27, 41
BtgI CCRYGG 1 cut(s) 153
CaiI CAGNNNCTG 2 cut(s) 211, 228
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 26
Cfr42I CCGCGG 1 cut(s) 156
Csp6I GTAC 2 cut(s) 4, 71
CviAII CATG 2 cut(s) 298, 307
CviJI RGCY 6 cut(s) 27, 41, 81, 121, 127, 164
CviKI_1 RGCY 6 cut(s) 27, 41, 81, 121, 127, 164
CviQI GTAC 2 cut(s) 4, 71
DdeI CTNAG 2 cut(s) 188, 260
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DrdI GACNNNNNNGTC 1 cut(s) 285
DseDI GACNNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 2 cut(s) 301, 310
FaiI YATR 3 cut(s) 118, 299, 308
FatI CATG 2 cut(s) 297, 306
FauI CCCGC 1 cut(s) 92
Fnu4HI GCNGC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 229
FspBI CTAG 1 cut(s) 319
GlaI GCGC 1 cut(s) 227
GluI GCNGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 233
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 27, 41
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 301, 310
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 3 cut(s) 173, 199, 247
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 185
Hpy188III TCNNGA 2 cut(s) 177, 290
Hpy8I GTNNAC 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 17, 260
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4IV ACGT 2 cut(s) 105, 286
HpyCH4V TGCA 3 cut(s) 138, 231, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 307
HpyF3I CTNAG 2 cut(s) 188, 260
HpySE526I ACGT 2 cut(s) 105, 286
Hsp92II CATG 2 cut(s) 301, 310
HspAI GCGC 1 cut(s) 226
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 7 cut(s) 124, 190, 191, 197, 208, 223, 315
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 1 cut(s) 319
MaeII ACGT 2 cut(s) 105, 286
MaeIII GTNAC 1 cut(s) 314
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MfeI CAATTG 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 2 cut(s) 8, 18
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 2 cut(s) 77, 173
Mox20I TGGCCA 1 cut(s) 41
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 155
MunI CAATTG 1 cut(s) 18
MvnI CGCG 2 cut(s) 60, 155
MwoI GCNNNNNNNGC 2 cut(s) 161, 307
NdeII GATC 1 cut(s) 191
NlaIII CATG 2 cut(s) 301, 310
NmuCI GTSAC 1 cut(s) 314
NspI RCATGY 2 cut(s) 301, 310
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 2 cut(s) 173, 199
PkrI GCNGC 1 cut(s) 230
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 106
PspPI GGNCC 1 cut(s) 26
PsrI GAACNNNNNNTAC 2 cut(s) 54, 86
PstNI CAGNNNCTG 2 cut(s) 211, 228
RsaI GTAC 2 cut(s) 5, 72
RsaNI GTAC 2 cut(s) 4, 71
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 1 cut(s) 191
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 241
SetI ASST 6 cut(s) 83, 108, 184, 271, 289, 316
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
Sse9I AATT 2 cut(s) 8, 18
SsiI CCGC 4 cut(s) 58, 85, 153, 155
SspMI CTAG 1 cut(s) 319
TaaI ACNGT 1 cut(s) 75
TaiI ACGT 2 cut(s) 108, 289
TaqI TCGA 3 cut(s) 159, 202, 291
TasI AATT 2 cut(s) 8, 18
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 173, 199
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 259
XceI RCATGY 2 cut(s) 301, 310
XspI CTAG 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.