pycom15g02040

Belongs to the bacterial ribosomal protein bL31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012462
Physical Location & Seq
Reverse (-)
1211268 .. 1211797
530 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGGCGCTCAATCTAACCACCTCATTCCTCCGAGGAACACCCTGCCCCTCTCTCCTCCCGCCCAAGAAGGTCTGCGGCGGCGGCGGGTCGTCGGCGGGGAATTGCCGCTCTGTTGTGACGTGCAGGAAGAAGGACATACACCCGCAGTTTTATGAGGACGCGAAGGTGTACTGCAAGGGGGAGCTGGTGATGACGACGGGTGGGACCCAGAAGGAGTACGTGGTGGACGTGTGGTCGGGCAACCACCCCTTCTACCTCGGAAACCGGTCGGGCATGCTTGTCGACGACGACCAGGTCGAGAAGTTCAGGAAGAAGTACGGCGAGCTGAGTCAGATCGATGGAGATCCCTGTCCTCAAGGGAGAGATTATTCTGCCCTCCCGCCGCAAAGCCGCCGCCGGCAAAGGCGGCAAGAAGAAGTAGAACAATGGTTTTCTTATTCCATGTAA

Protein Analysis

149

Amino Acids

16.68

Weight (kDa)

8.74

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L31 PF01197 43 - 107 2.7e-18 Ribosomal protein L31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012462)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75350
fragaria_vesca FvH4_2g39490 FvH4_2g39490
malus_domestica MD08G1027600.v1.1 MD15G1023800.v1.1
prunus_persica Prupe.1G376100_v2.0.a1
pyrus_communis pycom08g02160 pycom15g02040
rosa_chinensis RchiOBHm_Chr6g0301461
rosa_laevigata RLG00000011251
rosa_multiflora Rmu_co8470853.1_g000001 Rmu_sc0014417.1_g000013
rosa_roxburghii Rroxscaffold_7G00166780
rosa_rugosa Rorug06G0305000
rosa_samantha Rh6AG418600 Rh6BG424800 Rh6CG432100 Rh6DG418100
rosa_wichuraiana Rw6G036220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AccBSI CCGCTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 282
AccII CGCG 1 cut(s) 161
AclWI GGATC 1 cut(s) 338
AfaI GTAC 3 cut(s) 170, 218, 317
AflIII ACRYGT 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 264
AhdI GACNNNNNGTC 1 cut(s) 232
AjiI CACGTC 2 cut(s) 120, 229
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 2 cut(s) 184, 325
AluI AGCT 2 cut(s) 184, 325
AlwI GGATC 1 cut(s) 338
AsiGI ACCGGT 1 cut(s) 264
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 204
BccI CCATC 1 cut(s) 332
BceAI ACGGC 1 cut(s) 334
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 1 cut(s) 293
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 8 cut(s) 76, 79, 82, 106, 383, 391, 394, 407
BlsI GCNGC 8 cut(s) 77, 80, 83, 107, 384, 392, 395, 408
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 204
BmeRI GACNNNNNGTC 1 cut(s) 232
BmgBI CACGTC 2 cut(s) 120, 229
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 2 cut(s) 205, 206
BmrFI CCNGG 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 31, 256
BsaWI WCCGGW 1 cut(s) 264
Bse118I RCCGGY 2 cut(s) 264, 396
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 31, 256
BseJI GATNNNNATC 1 cut(s) 342
BseMII CTCAG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 265, 397
BslFI GGGAC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 217
Bsp143I GATC 2 cut(s) 333, 343
BspCNI CTCAG 1 cut(s) 318
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 205, 206
BspPI GGATC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 264, 396
BssAI RCCGGY 2 cut(s) 264, 396
BssECI CCNNGG 2 cut(s) 31, 256
BssMI GATC 2 cut(s) 333, 343
Bst2UI CCWGG 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 3 cut(s) 275, 323, 398
BstDEI CTNAG 1 cut(s) 326
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 336, 346
BstMBI GATC 2 cut(s) 333, 343
BstMCI CGRYCG 1 cut(s) 269
BstMWI GCNNNNNNNGC 2 cut(s) 81, 406
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 291
BstUI CGCG 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
Bsu15I ATCGAT 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 336
BtrI CACGTC 2 cut(s) 120, 229
Cac8I GCNNGC 3 cut(s) 275, 323, 398
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 264, 396
Cfr13I GGNCC 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 291
Csp6I GTAC 3 cut(s) 169, 217, 316
CspAI ACCGGT 1 cut(s) 264
CviAII CATG 2 cut(s) 274, 442
CviJI RGCY 3 cut(s) 184, 325, 390
CviKI_1 RGCY 3 cut(s) 184, 325, 390
CviQI GTAC 3 cut(s) 169, 217, 316
DdeI CTNAG 1 cut(s) 326
DpnI GATC 2 cut(s) 335, 345
DpnII GATC 2 cut(s) 333, 343
DrdI GACNNNNNNGTC 1 cut(s) 293
DriI GACNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1105I GACNNNNNGTC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 291
FaeI CATG 2 cut(s) 277, 445
FaiI YATR 4 cut(s) 137, 153, 275, 443
FaqI GGGAC 1 cut(s) 217
FatI CATG 2 cut(s) 273, 441
FauI CCCGC 5 cut(s) 66, 77, 88, 150, 387
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 8 cut(s) 76, 79, 82, 106, 383, 391, 394, 407
Fsp4HI GCNGC 8 cut(s) 76, 79, 82, 106, 383, 391, 394, 407
GlaI GCGC 1 cut(s) 6
GluI GCNGC 8 cut(s) 76, 79, 82, 106, 383, 391, 394, 407
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 2 cut(s) 265, 397
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 277, 445
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 1 cut(s) 328
HpaII CCGG 2 cut(s) 265, 397
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 3 cut(s) 169, 226, 283
Hpy188I TCNGA 3 cut(s) 32, 260, 333
Hpy188III TCNNGA 2 cut(s) 298, 307
Hpy8I GTNNAC 3 cut(s) 169, 226, 283
Hpy99I CGWCG 4 cut(s) 94, 199, 287, 290
HpyAV CCTTC 5 cut(s) 61, 124, 157, 205, 259
HpyCH4IV ACGT 3 cut(s) 119, 219, 228
HpyCH4V TGCA 2 cut(s) 123, 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 406
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 3 cut(s) 119, 219, 228
Hsp92II CATG 2 cut(s) 277, 445
HspAI GCGC 1 cut(s) 5
KflI GGGWCCC 1 cut(s) 204
KroI GCCGGC 1 cut(s) 396
KroNI GCCGGC 1 cut(s) 398
Kzo9I GATC 2 cut(s) 333, 343
LmnI GCTCC 1 cut(s) 181
MabI ACCWGGT 1 cut(s) 291
MaeII ACGT 3 cut(s) 119, 219, 228
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 335, 345
MbiI CCGCTC 1 cut(s) 108
MboI GATC 2 cut(s) 333, 343
MboII GAAGA 3 cut(s) 139, 322, 425
MflI RGATCY 1 cut(s) 343
MluCI AATT 1 cut(s) 100
MlyI GAGTC 1 cut(s) 337
MnlI CCTC 9 cut(s) 26, 31, 38, 58, 65, 148, 266, 363, 386
MroNI GCCGGC 1 cut(s) 396
MspI CCGG 2 cut(s) 265, 397
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 81, 406
NaeI GCCGGC 1 cut(s) 398
NdeII GATC 2 cut(s) 333, 343
NgoMIV GCCGGC 1 cut(s) 396
NlaIII CATG 2 cut(s) 277, 445
NlaIV GGNNCC 2 cut(s) 205, 206
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 294
PdiI GCCGGC 1 cut(s) 398
PflFI GACNNNGTC 1 cut(s) 293
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 8 cut(s) 77, 80, 83, 107, 384, 392, 395, 408
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 205, 206
PspPI GGNCC 1 cut(s) 204
PspPPI RGGWCCY 1 cut(s) 204
PsuI RGATCY 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 293
RsaI GTAC 3 cut(s) 170, 218, 317
RsaNI GTAC 3 cut(s) 169, 217, 316
SalI GTCGAC 1 cut(s) 281
SatI GCNGC 8 cut(s) 76, 79, 82, 106, 383, 391, 394, 407
Sau3AI GATC 2 cut(s) 333, 343
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 293
SexAI ACCWGGT 1 cut(s) 291
SinI GGWCC 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
SphI GCATGC 1 cut(s) 277
Sse9I AATT 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 291
TaiI ACGT 3 cut(s) 122, 222, 231
TaqI TCGA 3 cut(s) 282, 297, 336
TasI AATT 1 cut(s) 100
TatI WGTACW 1 cut(s) 168
TauI GCSGC 8 cut(s) 78, 81, 84, 108, 385, 393, 396, 409
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
Tth111I GACNNNGTC 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 204
XceI RCATGY 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.