pycom15g04000

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013219
Physical Location & Seq
Reverse (-)
2429657 .. 2432010
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGATAAGGGAAAGGGAGTGATGGGAGCAGGTCGGAGATGGGCCGTTGACTTGACTGACAACTCAACCTCCCCTTCTTCCCGCGACTTCCCCGACCCCCCTGGCTTCTCTCGCGCCTCCCTCGAACAGGATGATTCGACTGTGAGTCGCCAGAAGAAGGATGCCGAATCTACTTGGAAAGCTCAGAAAGCCTGGGAAGTTGCACAGGCCCCATTCAAGAATTTGATGATGATGGGTTTCATGATGTGGATGGCTGGGAATACAGTGCATTTGTTTAGCATTGGAATCACATTTTCCGCTCTTTGGCAACCCATAAGCGCCTTACAGGGTGTAGGGAAGGTTTTTGAGCCCTATAAGGACGGCAAAGTAGATCTTGTTGCCCCAAAGTTGCTCTTCATTGCCCTTAATTTAGGGGGTTTAGCATTGGGTGTTTGGAAGCTTAACACCTTGGGGTTGCTTCCTACACATGCATCGGATTGGGTTTCATCGTTACCCCCTGCTCAGGAGGTTGAGTATTCAGGTGGTGGCATTCCTCTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

181

Amino Acids

19.58

Weight (kDa)

6.73

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 52 - 164 2.8e-39 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10780
fragaria_vesca FvH4_3g24420 FvH4_6g27370
malus_domestica MD08G1052600.v1.1 MD15G1042900.v1.1
prunus_persica Prupe.1G396200_v2.0.a1
pyrus_communis pycom08g04390 pycom15g04000
rosa_chinensis RchiOBHm_Chr6g0299241
rosa_laevigata RLG00000011450
rosa_multiflora Rmu_sc0001434.1_g000007
rosa_roxburghii Rroxscaffold_7G00168860
rosa_rugosa Rorug06G0286500
rosa_samantha Rh6AG397800 Rh6BG406300 Rh6CG412400 Rh6DG399100
rosa_wichuraiana Rw6G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 20
AccBSI CCGCTC 1 cut(s) 299
AccII CGCG 2 cut(s) 84, 114
AciI CCGC 2 cut(s) 82, 297
AcsI RAATTY 1 cut(s) 220
AfiI CCNNNNNNNGG 4 cut(s) 127, 157, 303, 502
AgsI TTSAA 1 cut(s) 217
AhdI GACNNNNNGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 100, 191
AluBI AGCT 2 cut(s) 182, 439
AluI AGCT 2 cut(s) 182, 439
AoxI GGCC 2 cut(s) 42, 207
ApoI RAATTY 1 cut(s) 220
AspLEI GCGC 2 cut(s) 116, 320
AspS9I GGNCC 2 cut(s) 42, 208
BanII GRGCYC 1 cut(s) 351
BccI CCATC 4 cut(s) 16, 33, 226, 244
BceAI ACGGC 2 cut(s) 29, 376
BciT130I CCWGG 2 cut(s) 102, 193
BfoI RGCGCY 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 20
BglII AGATCT 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 102, 193
BmeRI GACNNNNNGTC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 42, 208
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 2 cut(s) 102, 193
BmsI GCATC 2 cut(s) 151, 479
Bpu10I CCTNAGC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 100, 192, 447
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 497, 527
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 157, 303, 502
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 2 cut(s) 102, 193
BseDI CCNNGG 3 cut(s) 100, 192, 447
BseGI GGATG 3 cut(s) 136, 166, 255
BseLI CCNNNNNNNGG 4 cut(s) 127, 157, 303, 502
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 197, 515
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 2 cut(s) 84, 114
BshFI GGCC 2 cut(s) 44, 209
BslI CCNNNNNNNGG 4 cut(s) 127, 157, 303, 502
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 2 cut(s) 44, 209
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 2 cut(s) 82, 297
BspANI GGCC 2 cut(s) 44, 209
BspCNI CTCAG 2 cut(s) 196, 514
BspFNI CGCG 2 cut(s) 84, 114
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 210
BspMI ACCTGC 1 cut(s) 20
BspQI GCTCTTC 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 100, 192, 447
BssMI GATC 1 cut(s) 370
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 2 cut(s) 102, 193
Bst4CI ACNGT 2 cut(s) 142, 265
Bst6I CTCTTC 1 cut(s) 398
BstDEI CTNAG 2 cut(s) 183, 501
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 3 cut(s) 136, 166, 255
BstFNI CGCG 2 cut(s) 84, 114
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 2 cut(s) 116, 320
BstKTI GATC 1 cut(s) 373
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 2 cut(s) 111, 188
BstNI CCWGG 2 cut(s) 102, 193
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 100, 191
BstUI CGCG 2 cut(s) 84, 114
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 2 cut(s) 44, 209
BtsCI GGATG 3 cut(s) 136, 166, 255
BtsIMutI CAGTG 1 cut(s) 270
BveI ACCTGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 240
CfoI GCGC 2 cut(s) 116, 320
Cfr13I GGNCC 2 cut(s) 42, 208
CviAII CATG 2 cut(s) 241, 467
CviJI RGCY 8 cut(s) 44, 105, 182, 191, 209, 254, 349, 439
CviKI_1 RGCY 8 cut(s) 44, 105, 182, 191, 209, 254, 349, 439
DdeI CTNAG 2 cut(s) 183, 501
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
DriI GACNNNNNGTC 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 398
Eam1105I GACNNNNNGTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 2 cut(s) 100, 191
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 2 cut(s) 244, 470
FaiI YATR 5 cut(s) 242, 314, 354, 468, 538
FalI AAGNNNNNCTT 4 cut(s) 357, 389, 377, 409
FatI CATG 2 cut(s) 240, 466
FauI CCCGC 1 cut(s) 89
FokI GGATG 3 cut(s) 143, 173, 262
FriOI GRGCYC 1 cut(s) 351
GlaI GCGC 2 cut(s) 115, 319
GsaI CCCAGC 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 2 cut(s) 44, 209
HhaI GCGC 2 cut(s) 116, 320
Hin1II CATG 2 cut(s) 244, 470
Hin6I GCGC 2 cut(s) 114, 318
HinP1I GCGC 2 cut(s) 114, 318
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 4 cut(s) 134, 145, 167, 285
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 36, 186, 475
Hpy188III TCNNGA 3 cut(s) 217, 241, 503
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 84, 151, 331
HpyCH4III ACNGT 2 cut(s) 142, 265
HpyCH4V TGCA 3 cut(s) 203, 268, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 188
HpyF3I CTNAG 2 cut(s) 183, 501
Hsp92II CATG 2 cut(s) 244, 470
HspAI GCGC 2 cut(s) 114, 318
Kzo9I GATC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 26
LweI GCATC 2 cut(s) 151, 479
MaeIII GTNAC 1 cut(s) 489
MalI GATC 1 cut(s) 372
MbiI CCGCTC 1 cut(s) 299
MboI GATC 1 cut(s) 370
MboII GAAGA 3 cut(s) 69, 166, 385
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 2 cut(s) 220, 406
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 5 cut(s) 79, 127, 131, 499, 543
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 2 cut(s) 405, 441
MspR9I CCNGG 2 cut(s) 102, 193
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 2 cut(s) 102, 193
MvnI CGCG 2 cut(s) 84, 114
MwoI GCNNNNNNNGC 2 cut(s) 111, 188
NdeII GATC 1 cut(s) 370
NlaIII CATG 2 cut(s) 244, 470
NlaIV GGNNCC 1 cut(s) 210
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 1 cut(s) 470
PagI TCATGA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 398
PctI GAATGC 1 cut(s) 528
PfeI GAWTC 3 cut(s) 134, 167, 285
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 100, 191
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 2 cut(s) 100, 191
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 2 cut(s) 42, 208
PsuI RGATCY 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 405, 441
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 2 cut(s) 42, 208
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 102, 193
SduI GDGCHC 1 cut(s) 351
SetI ASST 8 cut(s) 34, 71, 184, 342, 441, 449, 510, 523
SfaNI GCATC 2 cut(s) 151, 479
Sse9I AATT 2 cut(s) 220, 406
SsiI CCGC 2 cut(s) 82, 297
StyD4I CCNGG 2 cut(s) 100, 191
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 142, 265
TaqI TCGA 2 cut(s) 123, 137
TasI AATT 2 cut(s) 220, 406
TfiI GAWTC 3 cut(s) 134, 167, 285
Tru1I TTAA 2 cut(s) 405, 441
Tru9I TTAA 2 cut(s) 405, 441
TscAI CASTG 1 cut(s) 270
TspDTI ATGAA 3 cut(s) 229, 385, 474
TspRI CASTG 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 220
XceI RCATGY 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.