Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0012366

Physical Location & Seq

Genomic Coordinates Reverse (-)

4075670 .. 4076568

Gene Structure 899 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g06640.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 801 bp

ATGGTCAACACAATCCTCGAGGCTCTCAACGTCCGAGTCGTCGGCTCAGGCGACAGGTACGTCGTCTTCGCCCACGGCTTCGGCACCGACCAGTCCGCCTGGCAGCGCATTCTCCAGTTCTTCACGCCCTACTACCGCGTCATTCTCTACGACCTCGTCTGCGCCGGCAGCGTCAACCCGGACTACTTCGACTTCCGCCGTTACACCACCCTCGACGCCTATGTCGACGACCTCCTCAACATTCTCGACGCCCTCGGCGTCAACCACTGCGCCTATGTTGGCCACTCCGTCTCCGCCATGATCGGCATCCTCGCCTCCATCCGCCGCCCCGAACTCTTCTCCAAGCTCGTCCTCATCGGCGCTTCCCCAAGGTTCTTGAACGATGGAGATTACCATGGAGGATTCGAGCAAGAGGAGATTGAGAAAGTGTTTTCTGCAATGGAGGCGAACTACGCGGCGTGGGTCAGCGGCTTCGCGCCGCTAGCCGTCGGGGCCGACGTTCCGACGGTGGTCCGAGAATTCAGCCGGACCCTGTTCAACATGCGACCGGACATCACGCTTTTCGTGTCTCGGGCCGTATTCAACAGCGATTTGAGGGGGGTTTTGGGGCTGGTGCGGGTGCCGTGCTGCATAATCCAGACCTCGAAGGACGTGTCCGTACCGGGGTCAGTGGCGAACTACCTGAGGGACCACCTGGGCGGGCGGAACACGGTGGTGATGCTGGAAACGGAGGGACACTTGCCGCATCTGAGCGCGCCGGGTCTGCTGGCTAGGAAGCTCCGCCACGCCCTTGCGTCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009987 GO:0010016 GO:0010033 GO:0010223 GO:0010346 GO:0010817 GO:0014070 GO:0016106 GO:0016114 GO:0018130 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046483 GO:0048367 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:1901334 GO:1901336 GO:1901360 GO:1901362 GO:1901576 GO:1901600 GO:1901601 GO:1901700 GO:1901701 GO:1902347 GO:1902348 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

267

Amino Acids

29.37

Weight (kDa)

6.23

Isoelectric Point (pI)

34.85

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03990
fragaria_vesca	FvH4_2g33160
malus_domestica	MD08G1084500.v1.1 MD08G1084800.v1.1 MD15G1070700.v1.1
prunus_persica	Prupe.1G423400_v2.0.a1
pyrus_communis	pycom08g07000 pycom15g06640
rosa_chinensis	RchiOBHm_Chr6g0309501
rosa_laevigata	RLG00000010560
rosa_multiflora	Rmu_co8437415.1_g000001 Rmu_sc0007283.1_g000008
rosa_roxburghii	Rroxscaffold_7G00159350
rosa_rugosa	Rorug06G0372500
rosa_samantha	Rh6AG485800 Rh6BG495200 Rh6CG500200 Rh6DG486100
rosa_wichuraiana	Rw6G042310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	59, 221
AbsI	CCTCGAGG	1 cut(s)	17
AccB1I	GGYRCC	2 cut(s)	83, 619
AccI	GTMKAC	1 cut(s)	225
AccII	CGCG	4 cut(s)	138, 455, 476, 755
AcoI	YGGCCR	1 cut(s)	280
AcsI	RAATTY	1 cut(s)	518
AcyI	GRCGYC	3 cut(s)	216, 249, 258
AfaI	GTAC	2 cut(s)	59, 660
AfiI	CCNNNNNNNGG	1 cut(s)	663
AflIII	ACRYGT	1 cut(s)	651
AgsI	TTSAA	3 cut(s)	379, 538, 583
AjiI	CACGTC	1 cut(s)	652
AjnI	CCWGG	2 cut(s)	98, 693
AleI	CACNNNNGTG	1 cut(s)	713
AluBI	AGCT	2 cut(s)	346, 778
AluI	AGCT	2 cut(s)	346, 778
Alw26I	GTCTC	2 cut(s)	295, 573
Ama87I	CYCGRG	2 cut(s)	17, 570
AoxI	GGCC	3 cut(s)	280, 492, 573
ApeKI	GCWGC	3 cut(s)	103, 168, 627
ApoI	RAATTY	1 cut(s)	518
ArsI	GACNNNNNNTTYG	2 cut(s)	638, 670
AspLEI	GCGC	7 cut(s)	108, 164, 272, 362, 478, 755, 757
AspS9I	GGNCC	5 cut(s)	492, 511, 528, 573, 688
AsuC2I	CCSGG	3 cut(s)	179, 663, 759
AsuHPI	GGTGA	1 cut(s)	727
AsuNHI	GCTAGC	1 cut(s)	481
AvaI	CYCGRG	2 cut(s)	17, 570
AvaII	GGWCC	3 cut(s)	511, 528, 688
AxyI	CCTNAGG	1 cut(s)	683
BalI	TGGCCA	1 cut(s)	282
BanI	GGYRCC	2 cut(s)	83, 619
BarI	GAAGNNNNNNTAC	2 cut(s)	50, 82
BbsI	GAAGAC	1 cut(s)	58
BbvI	GCAGC	3 cut(s)	115, 180, 614
BccI	CCATC	2 cut(s)	326, 377
BceAI	ACGGC	5 cut(s)	91, 183, 470, 560, 607
BciT130I	CCWGG	2 cut(s)	100, 695
BcnI	CCSGG	3 cut(s)	179, 663, 759
BcoDI	GTCTC	2 cut(s)	295, 573
BfaI	CTAG	2 cut(s)	482, 771
BfoI	RGCGCY	1 cut(s)	363
BglI	GCCNNNNNGGC	1 cut(s)	491
BisI	GCNGC	8 cut(s)	104, 169, 325, 456, 469, 479, 628, 743
BlsI	GCNGC	8 cut(s)	105, 170, 326, 457, 470, 480, 629, 744
Bme1390I	CCNGG	5 cut(s)	100, 179, 663, 695, 759
Bme18I	GGWCC	3 cut(s)	511, 528, 688
BmeT110I	CYCGRG	2 cut(s)	17, 570
BmgBI	CACGTC	1 cut(s)	652
BmgT120I	GGNCC	5 cut(s)	492, 511, 528, 573, 688
BmiI	GGNNCC	5 cut(s)	85, 493, 530, 621, 689
BmrFI	CCNGG	5 cut(s)	100, 179, 663, 695, 759
BmsI	GCATC	3 cut(s)	315, 708, 754
BmtI	GCTAGC	1 cut(s)	485
BoxI	GACNNNNGTC	1 cut(s)	509
BpiI	GAAGAC	1 cut(s)	58
BpmI	CTGGAG	1 cut(s)	98
Bpu10I	CCTNAGC	1 cut(s)	46
BpuMI	CCSGG	3 cut(s)	179, 663, 759
BsaBI	GATNNNNATC	1 cut(s)	305
BsaHI	GRCGYC	3 cut(s)	216, 249, 258
BsaJI	CCNNGG	6 cut(s)	73, 253, 368, 394, 662, 694
BsaWI	WCCGGW	1 cut(s)	547
BsaXI	ACNNNNNCTCC	2 cut(s)	275, 305
Bsc4I	CCNNNNNNNGG	1 cut(s)	663
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	2 cut(s)	91, 115
Bse21I	CCTNAGG	1 cut(s)	683
Bse3DI	GCAATG	1 cut(s)	444
Bse8I	GATNNNNATC	1 cut(s)	305
BseBI	CCWGG	2 cut(s)	100, 695
BseDI	CCNNGG	6 cut(s)	73, 253, 368, 394, 662, 694
BseGI	GGATG	2 cut(s)	306, 318
BseJI	GATNNNNATC	1 cut(s)	305
BseLI	CCNNNNNNNGG	1 cut(s)	663
BseMI	GCAATG	1 cut(s)	444
BseMII	CTCAG	3 cut(s)	60, 674, 740
BseNI	ACTGG	2 cut(s)	91, 115
BsePI	GCGCGC	1 cut(s)	753
BseRI	GAGGAG	2 cut(s)	224, 428
BseXI	GCAGC	3 cut(s)	115, 180, 614
Bsh1236I	CGCG	4 cut(s)	138, 455, 476, 755
Bsh1285I	CGRYCG	1 cut(s)	548
BshFI	GGCC	3 cut(s)	282, 494, 575
BshNI	GGYRCC	2 cut(s)	83, 619
BsiEI	CGRYCG	1 cut(s)	548
BsiHKCI	CYCGRG	2 cut(s)	17, 570
BsiSI	CCGG	6 cut(s)	165, 179, 526, 548, 662, 758
BslFI	GGGAC	2 cut(s)	701, 747
BslI	CCNNNNNNNGG	1 cut(s)	663
BsmAI	GTCTC	2 cut(s)	295, 573
BsmBI	CGTCTC	1 cut(s)	295
BsmFI	GGGAC	2 cut(s)	701, 747
BsmI	GAATGC	1 cut(s)	108
BsnI	GGCC	3 cut(s)	282, 494, 575
BsoBI	CYCGRG	2 cut(s)	17, 570
Bsp143I	GATC	1 cut(s)	300
Bsp19I	CCATGG	1 cut(s)	394
BspANI	GGCC	3 cut(s)	282, 494, 575
BspCNI	CTCAG	3 cut(s)	59, 675, 741
BspFNI	CGCG	4 cut(s)	138, 455, 476, 755
BspLI	GGNNCC	5 cut(s)	85, 493, 530, 621, 689
BspOI	GCTAGC	1 cut(s)	485
BspT107I	GGYRCC	2 cut(s)	83, 619
BsrDI	GCAATG	1 cut(s)	444
BsrFI	RCCGGY	1 cut(s)	164
BsrI	ACTGG	2 cut(s)	91, 115
BssAI	RCCGGY	1 cut(s)	164
BssECI	CCNNGG	6 cut(s)	73, 253, 368, 394, 662, 694
BssHII	GCGCGC	1 cut(s)	753
BssMI	GATC	1 cut(s)	300
BssNI	GRCGYC	3 cut(s)	216, 249, 258
BssT1I	CCWWGG	2 cut(s)	368, 394
Bst2UI	CCWGG	2 cut(s)	100, 695
Bst4CI	ACNGT	2 cut(s)	508, 712
Bst6I	CTCTTC	1 cut(s)	341
BstACI	GRCGYC	3 cut(s)	216, 249, 258
BstC8I	GCNNGC	5 cut(s)	166, 483, 701, 755, 768
BstDEI	CTNAG	3 cut(s)	46, 683, 749
BstDSI	CCRYGG	2 cut(s)	73, 394
BstF5I	GGATG	2 cut(s)	306, 318
BstFNI	CGCG	4 cut(s)	138, 455, 476, 755
BstH2I	RGCGCY	1 cut(s)	363
BstHHI	GCGC	7 cut(s)	108, 164, 272, 362, 478, 755, 757
BstKTI	GATC	1 cut(s)	303
BstMAI	GTCTC	2 cut(s)	295, 573
BstMBI	GATC	1 cut(s)	300
BstMCI	CGRYCG	1 cut(s)	548
BstMWI	GCNNNNNNNGC	6 cut(s)	168, 443, 452, 482, 491, 763
BstNI	CCWGG	2 cut(s)	100, 695
BstNSI	RCATGY	1 cut(s)	544
BstPAI	GACNNNNGTC	1 cut(s)	509
BstSCI	CCNGG	5 cut(s)	98, 177, 661, 693, 757
BstUI	CGCG	4 cut(s)	138, 455, 476, 755
BstV1I	GCAGC	3 cut(s)	115, 180, 614
BstV2I	GAAGAC	1 cut(s)	58
Bsu36I	CCTNAGG	1 cut(s)	683
BsuRI	GGCC	3 cut(s)	282, 494, 575
BtgI	CCRYGG	2 cut(s)	73, 394
BtrI	CACGTC	1 cut(s)	652
BtsCI	GGATG	2 cut(s)	306, 318
BtsI	GCAGTG	1 cut(s)	265
BtsIMutI	CAGTG	2 cut(s)	265, 675
Cac8I	GCNNGC	5 cut(s)	166, 483, 701, 755, 768
CfoI	GCGC	7 cut(s)	108, 164, 272, 362, 478, 755, 757
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	5 cut(s)	492, 511, 528, 573, 688
CseI	GACGC	6 cut(s)	127, 160, 224, 247, 257, 783
Csp6I	GTAC	2 cut(s)	58, 659
CviAII	CATG	3 cut(s)	298, 395, 541
CviQI	GTAC	2 cut(s)	58, 659
DdeI	CTNAG	3 cut(s)	46, 683, 749
DpnI	GATC	1 cut(s)	302
DpnII	GATC	1 cut(s)	300
DrdI	GACNNNNNNGTC	2 cut(s)	59, 221
DseDI	GACNNNNNNGTC	2 cut(s)	59, 221
EaeI	YGGCCR	1 cut(s)	280
Eam1104I	CTCTTC	1 cut(s)	341
EarI	CTCTTC	1 cut(s)	341
EciI	GGCGGA	6 cut(s)	85, 185, 283, 311, 718, 770
Eco130I	CCWWGG	2 cut(s)	368, 394
Eco47I	GGWCC	3 cut(s)	511, 528, 688
Eco81I	CCTNAGG	1 cut(s)	683
Eco88I	CYCGRG	2 cut(s)	17, 570
EcoRI	GAATTC	1 cut(s)	518
EcoRII	CCWGG	2 cut(s)	98, 693
EcoT14I	CCWWGG	2 cut(s)	368, 394
ErhI	CCWWGG	2 cut(s)	368, 394
Esp3I	CGTCTC	1 cut(s)	295
FaeI	CATG	3 cut(s)	301, 398, 544
FaiI	YATR	6 cut(s)	222, 276, 299, 396, 542, 632
FaqI	GGGAC	2 cut(s)	701, 747
FatI	CATG	3 cut(s)	297, 394, 540
FauI	CCCGC	2 cut(s)	609, 692
FblI	GTMKAC	1 cut(s)	225
Fnu4HI	GCNGC	8 cut(s)	104, 169, 325, 456, 469, 479, 628, 743
FokI	GGATG	2 cut(s)	293, 305
Fsp4HI	GCNGC	8 cut(s)	104, 169, 325, 456, 469, 479, 628, 743
FspBI	CTAG	2 cut(s)	482, 771
GlaI	GCGC	7 cut(s)	107, 163, 271, 361, 477, 754, 756
GluI	GCNGC	8 cut(s)	104, 169, 325, 456, 469, 479, 628, 743
GsuI	CTGGAG	1 cut(s)	98
HaeII	RGCGCY	1 cut(s)	363
HaeIII	GGCC	3 cut(s)	282, 494, 575
HapII	CCGG	6 cut(s)	165, 179, 526, 548, 662, 758
HgaI	GACGC	6 cut(s)	127, 160, 224, 247, 257, 783
HhaI	GCGC	7 cut(s)	108, 164, 272, 362, 478, 755, 757
Hin1I	GRCGYC	3 cut(s)	216, 249, 258
Hin1II	CATG	3 cut(s)	301, 398, 544
Hin6I	GCGC	7 cut(s)	106, 162, 270, 360, 476, 753, 755
HinP1I	GCGC	7 cut(s)	106, 162, 270, 360, 476, 753, 755
HincII	GTYRAC	4 cut(s)	7, 175, 226, 262
HindII	GTYRAC	4 cut(s)	7, 175, 226, 262
HinfI	GANTC	2 cut(s)	36, 402
HpaII	CCGG	6 cut(s)	165, 179, 526, 548, 662, 758
HphI	GGTGA	1 cut(s)	727
Hpy166II	GTNNAC	4 cut(s)	7, 175, 226, 262
Hpy188I	TCNGA	4 cut(s)	35, 504, 515, 750
Hpy188III	TCNNGA	3 cut(s)	245, 376, 637
Hpy8I	GTNNAC	4 cut(s)	7, 175, 226, 262
Hpy99I	CGWCG	9 cut(s)	44, 65, 218, 230, 251, 491, 500, 508, 799
HpyAV	CCTTC	1 cut(s)	640
HpyCH4III	ACNGT	2 cut(s)	508, 712
HpyCH4IV	ACGT	4 cut(s)	30, 60, 498, 651
HpyCH4V	TGCA	2 cut(s)	437, 630
HpyF10VI	GCNNNNNNNGC	6 cut(s)	168, 443, 452, 482, 491, 763
HpyF3I	CTNAG	3 cut(s)	46, 683, 749
HpySE526I	ACGT	4 cut(s)	30, 60, 498, 651
Hsp92I	GRCGYC	3 cut(s)	216, 249, 258
Hsp92II	CATG	3 cut(s)	301, 398, 544
HspAI	GCGC	7 cut(s)	106, 162, 270, 360, 476, 753, 755
KroI	GCCGGC	1 cut(s)	164
KroNI	GCCGGC	1 cut(s)	166
Kzo9I	GATC	1 cut(s)	300
LmnI	GCTCC	1 cut(s)	783
Lsp1109I	GCAGC	3 cut(s)	115, 180, 614
LweI	GCATC	3 cut(s)	315, 708, 754
MaeI	CTAG	2 cut(s)	482, 771
MaeII	ACGT	4 cut(s)	30, 60, 498, 651
MaeIII	GTNAC	1 cut(s)	200
MalI	GATC	1 cut(s)	302
MboI	GATC	1 cut(s)	300
MboII	GAAGA	3 cut(s)	58, 112, 328
MlsI	TGGCCA	1 cut(s)	282
MluCI	AATT	1 cut(s)	518
MluNI	TGGCCA	1 cut(s)	282
MlyI	GAGTC	1 cut(s)	45
MmeI	TCCRAC	1 cut(s)	527
Mox20I	TGGCCA	1 cut(s)	282
MroNI	GCCGGC	1 cut(s)	164
MscI	TGGCCA	1 cut(s)	282
MslI	CAYNNNNRTG	1 cut(s)	713
Msp20I	TGGCCA	1 cut(s)	282
MspA1I	CMGCKG	1 cut(s)	468
MspI	CCGG	6 cut(s)	165, 179, 526, 548, 662, 758
MspR9I	CCNGG	5 cut(s)	100, 179, 663, 695, 759
Mva1269I	GAATGC	1 cut(s)	108
MvaI	CCWGG	2 cut(s)	100, 695
MvnI	CGCG	4 cut(s)	138, 455, 476, 755
MwoI	GCNNNNNNNGC	6 cut(s)	168, 443, 452, 482, 491, 763
NaeI	GCCGGC	1 cut(s)	166
NciI	CCSGG	3 cut(s)	179, 663, 759
NcoI	CCATGG	1 cut(s)	394
NdeII	GATC	1 cut(s)	300
NgoMIV	GCCGGC	1 cut(s)	164
NheI	GCTAGC	1 cut(s)	481
NlaIII	CATG	3 cut(s)	301, 398, 544
NlaIV	GGNNCC	5 cut(s)	85, 493, 530, 621, 689
NmeAIII	GCCGAG	1 cut(s)	234
NspI	RCATGY	1 cut(s)	544
OliI	CACNNNNGTG	1 cut(s)	713
PaeR7I	CTCGAG	1 cut(s)	17
PauI	GCGCGC	1 cut(s)	753
PcsI	WCGNNNNNNNCGW	5 cut(s)	36, 48, 222, 255, 495
PctI	GAATGC	1 cut(s)	108
PdiI	GCCGGC	1 cut(s)	166
PfeI	GAWTC	1 cut(s)	402
PflFI	GACNNNGTC	1 cut(s)	155
PkrI	GCNGC	8 cut(s)	105, 170, 326, 457, 470, 480, 629, 744
PleI	GAGTC	1 cut(s)	44
PpsI	GAGTC	1 cut(s)	44
PshAI	GACNNNNGTC	1 cut(s)	509
Psp6I	CCWGG	2 cut(s)	98, 693
PspGI	CCWGG	2 cut(s)	98, 693
PspN4I	GGNNCC	5 cut(s)	85, 493, 530, 621, 689
PspPI	GGNCC	5 cut(s)	492, 511, 528, 573, 688
PspXI	VCTCGAGB	1 cut(s)	17
PsyI	GACNNNGTC	1 cut(s)	155
PteI	GCGCGC	1 cut(s)	753
RsaI	GTAC	2 cut(s)	59, 660
RsaNI	GTAC	2 cut(s)	58, 659
RseI	CAYNNNNRTG	1 cut(s)	713
SalI	GTCGAC	1 cut(s)	224
SatI	GCNGC	8 cut(s)	104, 169, 325, 456, 469, 479, 628, 743
Sau3AI	GATC	1 cut(s)	300
Sau96I	GGNCC	5 cut(s)	492, 511, 528, 573, 688
SchI	GAGTC	1 cut(s)	45
ScrFI	CCNGG	5 cut(s)	100, 179, 663, 695, 759
SfaNI	GCATC	3 cut(s)	315, 708, 754
Sfr274I	CTCGAG	1 cut(s)	17
SinI	GGWCC	3 cut(s)	511, 528, 688
SlaI	CTCGAG	1 cut(s)	17
SmiMI	CAYNNNNRTG	1 cut(s)	713
SmlI	CTYRAG	1 cut(s)	17
SmoI	CTYRAG	1 cut(s)	17
Sse9I	AATT	1 cut(s)	518
SspMI	CTAG	2 cut(s)	482, 771
StyD4I	CCNGG	5 cut(s)	98, 177, 661, 693, 757
StyI	CCWWGG	2 cut(s)	368, 394
TaaI	ACNGT	2 cut(s)	508, 712
TaiI	ACGT	4 cut(s)	33, 63, 501, 654
TaqI	TCGA	7 cut(s)	18, 189, 213, 225, 246, 405, 644
TasI	AATT	1 cut(s)	518
TauI	GCSGC	5 cut(s)	327, 458, 471, 481, 745
TfiI	GAWTC	1 cut(s)	402
TscAI	CASTG	2 cut(s)	272, 675
TseI	GCWGC	3 cut(s)	103, 168, 627
TspGWI	ACGGA	3 cut(s)	277, 646, 743
TspRI	CASTG	2 cut(s)	272, 675
Tth111I	GACNNNGTC	1 cut(s)	155
VpaK11BI	GGWCC	3 cut(s)	511, 528, 688
XapI	RAATTY	1 cut(s)	518
XceI	RCATGY	1 cut(s)	544
XhoI	CTCGAG	1 cut(s)	17
XmiI	GTMKAC	1 cut(s)	225
XspI	CTAG	2 cut(s)	482, 771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.