pycom15g08250

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013549
Physical Location & Seq
Forward (+)
5153077 .. 5153781
705 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGCCGTCTGTGCACTCTAGCCCCTTCCACCACATAGAGAACTCAGCCATATTTAACCTAATCCGGCACGCTCACACGCCGGGCCAAAAACGTTCAACAAAATCTTCATCCGGAGGCCTTCTCAAGATGTTCAAGCTCTTTCCCATGTTAACATCAGGCTGCAAAATGGTTGCCTTGCTAGGCAGGCCCCGGAAGCCGTTACTGAAGGATCATGCCACAACAGGTACAATTTTCGGCTATCGAAAGGGGAGGGTAAGCCTAGCCATACAAGAAGACCCTCATTGCATGCCACTCTTTGTGATAGAGCTGCCAATGCACAGTAGTGTGTTTCACAAGGAAATGGCGTCGGATATAGTCCGAATCGCGCTGGAGAGCGAGACGAAGACACACAAGAAGAAGCTTATGGAGGAGTTTGTGTGGGCTGTATACTGCAATGGAAGAAAGGTAGGGTATTCTATTAGGAGGAAGCAAATGTCGGAGGATGAGCTTCATGTTATGCAAACTTTGAGGGGTGTTTCGATGGGGGCAGGGGTTCTTCCATGCCCTTCTGATCAGAAGGAGTATGCAGGAGATGGGGAGTTGACTTACATTAGGGCAAGGTTTGAGAGGGTGGTTCGATCAAAGGACTCTGAAGCTTTGTACATGATTAATCCAGATGGTGCTGCAGGGCCAGAATTAAGTATTTTCTTTGTCAGAGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

235

Amino Acids

26.31

Weight (kDa)

9.53

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 74 - 232 6.4e-67 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42680 AT5G42680
fragaria_vesca FvH4_2g34960
malus_domestica MD08G1107300.v1.1 MD15G1087900.v1.1
prunus_persica Prupe.1G441600_v2.0.a1
pyrus_communis pycom08g08920 pycom15g08250
rosa_chinensis RchiOBHm_Chr6g0307041
rosa_laevigata RLG00000010741
rosa_multiflora Rmu_sc0005634.1_g000008
rosa_roxburghii Rroxscaffold_7G00161220
rosa_rugosa Rorug06G0355400
rosa_samantha Rh6AG466800 Rh6BG433600 Rh6CG482400 Rh6DG468600
rosa_wichuraiana Rw6G040690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 365
AccIII TCCGGA 1 cut(s) 110
AclI AACGTT 1 cut(s) 91
AclWI GGATC 1 cut(s) 216
AcuI CTGAAG 2 cut(s) 224, 651
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 2 cut(s) 226, 641
AgsI TTSAA 2 cut(s) 96, 133
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 6 cut(s) 136, 307, 400, 487, 635, 698
AluI AGCT 6 cut(s) 136, 307, 400, 487, 635, 698
Alw21I GWGCWC 2 cut(s) 15, 700
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 11
AlwI GGATC 1 cut(s) 216
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 4 cut(s) 82, 115, 185, 668
ApaLI GTGCAC 1 cut(s) 11
ApeKI GCWGC 3 cut(s) 159, 307, 662
AseI ATTAAT 1 cut(s) 648
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 3 cut(s) 82, 186, 668
AsuC2I CCSGG 2 cut(s) 81, 190
BaeGI GKGCMC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 700
BbsI GAAGAC 2 cut(s) 279, 389
Bbv12I GWGCWC 2 cut(s) 15, 700
BbvI GCAGC 3 cut(s) 146, 294, 649
BccI CCATC 3 cut(s) 514, 566, 650
BceAI ACGGC 1 cut(s) 181
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 2 cut(s) 81, 190
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 3 cut(s) 18, 179, 260
BfmI CTRYAG 1 cut(s) 663
BisI GCNGC 3 cut(s) 160, 308, 663
BlsI GCNGC 3 cut(s) 161, 309, 664
Bme1390I CCNGG 2 cut(s) 81, 190
BmgT120I GGNCC 3 cut(s) 82, 186, 668
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 2 cut(s) 81, 190
BpiI GAAGAC 2 cut(s) 279, 389
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 2 cut(s) 81, 190
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bse3DI GCAATG 2 cut(s) 280, 439
BseAI TCCGGA 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 2 cut(s) 107, 487
BseMI GCAATG 2 cut(s) 280, 439
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 15
BseXI GCAGC 3 cut(s) 146, 294, 649
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 4 cut(s) 84, 117, 187, 670
BsiHKAI GWGCWC 2 cut(s) 15, 700
BsiSI CCGG 4 cut(s) 64, 80, 111, 190
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 4 cut(s) 84, 117, 187, 670
Bsp1286I GDGCHC 2 cut(s) 15, 700
Bsp13I TCCGGA 1 cut(s) 110
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 3 cut(s) 208, 550, 617
BspANI GGCC 4 cut(s) 84, 117, 187, 670
BspCNI CTCAG 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 110
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 1 cut(s) 667
BspPI GGATC 1 cut(s) 216
BsrDI GCAATG 2 cut(s) 280, 439
BsrGI TGTACA 1 cut(s) 639
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 208, 550, 617
BssNAI GTATAC 1 cut(s) 427
BssNI GRCGYC 1 cut(s) 344
Bst1107I GTATAC 1 cut(s) 427
Bst4CI ACNGT 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 639
BstC8I GCNNGC 3 cut(s) 69, 185, 287
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 107, 487
BstFNI CGCG 1 cut(s) 365
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 3 cut(s) 211, 553, 620
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 3 cut(s) 208, 550, 617
BstMWI GCNNNNNNNGC 4 cut(s) 10, 184, 193, 313
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 79, 188
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 15
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 146, 294, 649
BstV2I GAAGAC 2 cut(s) 279, 389
BstZ17I GTATAC 1 cut(s) 427
BsuRI GGCC 4 cut(s) 84, 117, 187, 670
BtsCI GGATG 2 cut(s) 107, 487
Cac8I GCNNGC 3 cut(s) 69, 185, 287
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 82, 186, 668
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 225, 640
CviAII CATG 6 cut(s) 145, 212, 286, 491, 540, 643
CviQI GTAC 2 cut(s) 225, 640
DdeI CTNAG 1 cut(s) 43
DpnI GATC 3 cut(s) 210, 552, 619
DpnII GATC 3 cut(s) 208, 550, 617
Ecl136II GAGCTC 1 cut(s) 698
Eco147I AGGCCT 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 700
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 2 cut(s) 224, 651
EcoICRI GAGCTC 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 700
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 6 cut(s) 148, 215, 289, 494, 543, 646
FatI CATG 6 cut(s) 144, 211, 285, 490, 539, 642
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 160, 308, 663
FokI GGATG 2 cut(s) 94, 494
FriOI GRGCYC 1 cut(s) 700
Fsp4HI GCNGC 3 cut(s) 160, 308, 663
FspBI CTAG 3 cut(s) 18, 179, 260
GlaI GCGC 1 cut(s) 366
GluI GCNGC 3 cut(s) 160, 308, 663
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 4 cut(s) 84, 117, 187, 670
HapII CCGG 4 cut(s) 64, 80, 111, 190
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 367
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 6 cut(s) 148, 215, 289, 494, 543, 646
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 2 cut(s) 150, 582
HindII GTYRAC 2 cut(s) 150, 582
HindIII AAGCTT 2 cut(s) 398, 633
HinfI GANTC 2 cut(s) 360, 626
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 4 cut(s) 64, 80, 111, 190
Hpy166II GTNNAC 4 cut(s) 13, 150, 427, 582
Hpy188I TCNGA 7 cut(s) 349, 359, 478, 550, 555, 631, 695
Hpy188III TCNNGA 3 cut(s) 111, 124, 653
Hpy8I GTNNAC 4 cut(s) 13, 150, 427, 582
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 5 cut(s) 34, 128, 199, 550, 555
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 8 cut(s) 13, 162, 285, 316, 432, 499, 566, 665
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 184, 193, 313
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 1 cut(s) 91
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 6 cut(s) 148, 215, 289, 494, 543, 646
HspAI GCGC 1 cut(s) 365
Kpn2I TCCGGA 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 208, 550, 617
Lsp1109I GCAGC 3 cut(s) 146, 294, 649
MaeI CTAG 3 cut(s) 18, 179, 260
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 1 cut(s) 198
MalI GATC 3 cut(s) 210, 552, 619
MboI GATC 3 cut(s) 208, 550, 617
MboII GAAGA 6 cut(s) 96, 284, 394, 406, 450, 527
MhlI GDGCHC 2 cut(s) 15, 700
MluCI AATT 2 cut(s) 228, 674
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 2 cut(s) 327, 456
MnlI CCTC 8 cut(s) 107, 243, 288, 400, 456, 472, 501, 600
MroI TCCGGA 1 cut(s) 110
MseI TTAA 4 cut(s) 54, 149, 648, 677
MslI CAYNNNNRTG 1 cut(s) 321
MspI CCGG 4 cut(s) 64, 80, 111, 190
MspR9I CCNGG 2 cut(s) 81, 190
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 4 cut(s) 10, 184, 193, 313
NciI CCSGG 2 cut(s) 81, 190
NdeII GATC 3 cut(s) 208, 550, 617
NlaIII CATG 6 cut(s) 148, 215, 289, 494, 543, 646
NlaIV GGNNCC 1 cut(s) 188
NspI RCATGY 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 321
PaeI GCATGC 1 cut(s) 289
PceI AGGCCT 1 cut(s) 117
PfeI GAWTC 1 cut(s) 360
PkrI GCNGC 3 cut(s) 161, 309, 664
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
PshBI ATTAAT 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 700
Psp1406I AACGTT 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 3 cut(s) 82, 186, 668
PstI CTGCAG 1 cut(s) 667
RsaI GTAC 2 cut(s) 226, 641
RsaNI GTAC 2 cut(s) 225, 640
RseI CAYNNNNRTG 1 cut(s) 321
SacI GAGCTC 1 cut(s) 700
SaqAI TTAA 4 cut(s) 54, 149, 648, 677
SatI GCNGC 3 cut(s) 160, 308, 663
Sau3AI GATC 3 cut(s) 208, 550, 617
Sau96I GGNCC 3 cut(s) 82, 186, 668
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 81, 190
SduI GDGCHC 2 cut(s) 15, 700
SfcI CTRYAG 1 cut(s) 663
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 289
Sse9I AATT 2 cut(s) 228, 674
SseBI AGGCCT 1 cut(s) 117
SspMI CTAG 3 cut(s) 18, 179, 260
SstI GAGCTC 1 cut(s) 700
StuI AGGCCT 1 cut(s) 117
StyD4I CCNGG 2 cut(s) 79, 188
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 94
TaqI TCGA 3 cut(s) 241, 518, 616
TasI AATT 2 cut(s) 228, 674
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 1 cut(s) 360
Tru1I TTAA 4 cut(s) 54, 149, 648, 677
Tru9I TTAA 4 cut(s) 54, 149, 648, 677
TseI GCWGC 3 cut(s) 159, 307, 662
TspDTI ATGAA 2 cut(s) 96, 479
VneI GTGCAC 1 cut(s) 11
VspI ATTAAT 1 cut(s) 648
XceI RCATGY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 426
XspI CTAG 3 cut(s) 18, 179, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.