pycom15g11530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017248
Physical Location & Seq
Forward (+)
7800622 .. 7802497
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1611 bp
ATGTGTGGTTTGAACGGTTGGTCGGCTCCTGCCCTTCATAGACTGGTAGTTCTAGGCTCTTTCACCAACACCTGTAACTCACAGAACTTCAAACCCAAGCAAACCAAAACCATGGCCGACGCCACGGAGGAGCAAGATAACCACCACGTTCATCCGCTGCTAGAATCTCTGAAGAAAGCTTCCAAAGACCTACAGAGCAACCCTATTTCAATCATATACAACAACTACGACATCAACGCCGCCATTGAGGCACTTCTCGAGCTCGGAACCAAGGCCGACCCCATCATCTCCGGCGACCCAAGTCTCTTCACCCTCAACCAGCTGCTCAGCAACCTCAAAACCCTTCTCGAAAAGCTCCAAAAGCTGCAGGGTTACGGCCTCCGGTCCCTCCTCCACCGCCAAGTCACCAATTACAAGATTTCCCAAATTGGGTACGCCATGGAAGCGGAGGTTCAGGCGCACATCGATCGGAAGAACGTCTGGGGTTTCGTGAAAACGATGGAGGAGGATTCGGAGGACGATTGGAAAGTTAAGGCATTGATGATTTTAGAGAACAGATTGAACCAGGGATTCGATAAAGATTACCAAGAACTAATTCTGAGAGCAAAGGTATTTTCAATTCTTGAATTTATTACTTGTGATTCAAGTTTTTCTAATCGGGTTCGAGACCGGGCTGCCCTCGCTGTTCTCGCTCTGGTTAAATTCAACAAAGATGTTTTTGTTGGATTGGTTTTAATGGGTCCGATCGTTCGAGCTTTAATTCAAATGGGTTCGAGCTGTTCGATTCAAGTTCTCACTTGGCTGGTGAAAATCATCAGAACTCCTTTGGTGGATGACATCAATGGAGAAATCCCAAGAATTATCAGCCTCCTGGGGGCGGATGACATGGCGGTTCGAGCGGCAGCGATGAACTGCGTTCTTGAAATTGCGTTTCTGGGGAGAGAGGAAGTGATCGAATTGATGCTTGGGGAGGACTTGATCGAGAAGCTCATGAATTTACAGAGGTTGGAGACAAAAATGGAGGAAGGAGAGGGGAAGTTTCCTTTTTCGAGGTGCGTAGCGGGGTTTGCAGTGCTGGTGGAGGTTGGGCACGGGTTGGAGGAGCAGAGTGAGAAAAGAGGGTTTAAGATGGAAATATTGAGGAGAGTGAGGGAGGCCTCTGTTTCTGATGCAGAGGCTGCCTCTGTTGTTGCAGAGGTTTTGTGGGAAACATCGGCTGAAGACGGAGGCTTGTTGGCTACTGGGAGTGGGAGCTCATCTACTCAAATATCTGTTTCAGCCGCTGAAAGTATACAGTCACCTTCATGTTTTGTTCGAGTTAGGAACATACCGATATGCTCTTATCCAGCTCAATCTGAAGGCCGTTCAGGAGACTCTGAATTGTTGGAATCCAAATGCAAGAAATTTCAGAAGAACTTTGATCTCGAACTTCCTGCCGATGCATACACTGATGATGAAAGAGCACGATTCTTTGTAGACGGAAAGGTTTCTGAAGCACCTAAGGGGGCAAGTTCTCGTTTGAAAAGATTTCCAGAGGTTGTGTGTAATGGTGATGCAAAACATAGTGGATATTGTTCTATTTCTGCATCATTAGGAAAAGATTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

537

Amino Acids

59.2

Weight (kDa)

5.4

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 422 - 503 3.1e-07 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g28800
malus_domestica MD15G1128100.v1.1
prunus_persica Prupe.1G483400_v2.0.a1
pyrus_communis pycom15g11530
rosa_chinensis RchiOBHm_Chr6g0297551
rosa_laevigata RLG00000011584
rosa_multiflora Rmu_ssc0000319.1_g000004
rosa_roxburghii Rroxscaffold_7G00170370
rosa_rugosa Rorug06G0273500
rosa_samantha Rh6AG384500 Rh6BG392600 Rh6DG385000
rosa_wichuraiana Rw6G033490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 899
AccI GTMKAC 2 cut(s) 1291, 1476
AciI CCGC 9 cut(s) 155, 240, 397, 446, 878, 890, 899, 1061, 1281
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 4 cut(s) 626, 701, 994, 1403
AcuI CTGAAG 4 cut(s) 191, 1239, 1377, 1512
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 429, 874, 877
AjnI CCWGG 2 cut(s) 564, 870
AjuI GAANNNNNNNTTGG 4 cut(s) 579, 611, 948, 980
Alw21I GWGCWC 3 cut(s) 264, 1256, 1465
Alw26I GTCTC 4 cut(s) 308, 660, 1004, 1365
AlwNI CAGNNNCTG 2 cut(s) 1178, 1283
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 5 cut(s) 114, 273, 376, 1155, 1360
ApeKI GCWGC 6 cut(s) 157, 322, 364, 674, 902, 1178
ApoI RAATTY 4 cut(s) 626, 701, 994, 1403
Asp700I GAANNNNTTC 3 cut(s) 594, 1486, 1600
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 384, 740
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 6 cut(s) 55, 301, 397, 817, 1290, 1562
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 384, 740
AxyI CCTNAGG 1 cut(s) 1500
BaeGI GKGCMC 1 cut(s) 1092
BanII GRGCYC 2 cut(s) 264, 1256
BbsI GAAGAC 1 cut(s) 1227
Bbv12I GWGCWC 3 cut(s) 264, 1256, 1465
BbvI GCAGC 6 cut(s) 144, 309, 351, 661, 914, 1165
BccI CCATC 3 cut(s) 290, 493, 1123
BceAI ACGGC 2 cut(s) 391, 1347
BcgI CGANNNNNNTGC 4 cut(s) 449, 483, 1415, 1449
BciT130I CCWGG 2 cut(s) 566, 872
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 4 cut(s) 308, 660, 1004, 1365
BfaI CTAG 2 cut(s) 53, 161
BfmI CTRYAG 2 cut(s) 191, 365
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 9 cut(s) 158, 240, 323, 365, 675, 900, 903, 1179, 1281
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 9 cut(s) 159, 241, 324, 366, 676, 901, 904, 1180, 1282
Bme1390I CCNGG 3 cut(s) 566, 671, 872
Bme18I GGWCC 2 cut(s) 384, 740
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 384, 740
BmiI GGNNCC 4 cut(s) 27, 268, 386, 741
BmrFI CCNGG 3 cut(s) 566, 671, 872
BmrI ACTGGG 1 cut(s) 1251
BmsI GCATC 5 cut(s) 951, 1159, 1429, 1543, 1595
BmuI ACTGGG 1 cut(s) 1251
BoxI GACNNNNGTC 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 1227
BplI GAGNNNNNCTC 2 cut(s) 1166, 1198
Bpu1102I GCTNAGC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 6 cut(s) 111, 123, 270, 438, 565, 871
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 874, 877
Bse1I ACTGG 2 cut(s) 48, 1246
Bse21I CCTNAGG 1 cut(s) 1500
BseBI CCWGG 2 cut(s) 566, 872
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 6 cut(s) 111, 123, 270, 438, 565, 871
BseGI GGATG 3 cut(s) 151, 838, 886
BseLI CCNNNNNNNGG 3 cut(s) 429, 874, 877
BseMII CTCAG 2 cut(s) 340, 590
BseNI ACTGG 2 cut(s) 48, 1246
BseRI GAGGAG 5 cut(s) 143, 380, 518, 1115, 1156
BseSI GKGCMC 1 cut(s) 1092
BseXI GCAGC 6 cut(s) 144, 309, 351, 661, 914, 1165
Bsh1285I CGRYCG 2 cut(s) 469, 747
BshFI GGCC 5 cut(s) 116, 275, 378, 1157, 1362
BshVI ATCGAT 1 cut(s) 465
BsiEI CGRYCG 2 cut(s) 469, 747
BsiHKAI GWGCWC 3 cut(s) 264, 1256, 1465
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 291, 382, 670
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 429, 874, 877
BsmAI GTCTC 4 cut(s) 308, 660, 1004, 1365
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 5 cut(s) 116, 275, 378, 1157, 1362
Bso31I GGTCTC 1 cut(s) 660
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 4 cut(s) 264, 1092, 1256, 1465
Bsp143I GATC 5 cut(s) 466, 744, 951, 978, 1420
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp19I CCATGG 2 cut(s) 111, 438
BspACI CCGC 9 cut(s) 155, 240, 397, 446, 878, 890, 899, 1061, 1281
BspANI GGCC 5 cut(s) 116, 275, 378, 1157, 1362
BspCNI CTCAG 2 cut(s) 339, 591
BspDI ATCGAT 1 cut(s) 465
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 4 cut(s) 27, 268, 386, 741
BspMAI CTGCAG 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 660
BsrBI CCGCTC 1 cut(s) 899
BsrI ACTGG 2 cut(s) 48, 1246
BssECI CCNNGG 6 cut(s) 111, 123, 270, 438, 565, 871
BssMI GATC 5 cut(s) 466, 744, 951, 978, 1420
BssNAI GTATAC 1 cut(s) 1292
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 3 cut(s) 111, 270, 438
Bst1107I GTATAC 1 cut(s) 1292
Bst2UI CCWGG 2 cut(s) 566, 872
Bst4CI ACNGT 2 cut(s) 17, 1296
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstDEI CTNAG 3 cut(s) 326, 599, 1500
BstDSI CCRYGG 3 cut(s) 111, 123, 438
BstF5I GGATG 3 cut(s) 151, 838, 886
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 5 cut(s) 469, 747, 954, 981, 1423
BstMAI GTCTC 4 cut(s) 308, 660, 1004, 1365
BstMBI GATC 5 cut(s) 466, 744, 951, 978, 1420
BstMCI CGRYCG 2 cut(s) 469, 747
BstMWI GCNNNNNNNGC 8 cut(s) 248, 361, 443, 680, 689, 896, 1067, 1178
BstNI CCWGG 2 cut(s) 566, 872
BstPAI GACNNNNGTC 1 cut(s) 300
BstSCI CCNGG 3 cut(s) 564, 669, 870
BstSFI CTRYAG 2 cut(s) 191, 365
BstSLI GKGCMC 1 cut(s) 1092
BstV1I GCAGC 6 cut(s) 144, 309, 351, 661, 914, 1165
BstV2I GAAGAC 1 cut(s) 1227
BstXI CCANNNNNNTGG 1 cut(s) 112
BstZ17I GTATAC 1 cut(s) 1292
Bsu15I ATCGAT 1 cut(s) 465
Bsu36I CCTNAGG 1 cut(s) 1500
BsuRI GGCC 5 cut(s) 116, 275, 378, 1157, 1362
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 3 cut(s) 111, 123, 438
BtgZI GCGATG 1 cut(s) 920
BtsCI GGATG 3 cut(s) 151, 838, 886
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 2 cut(s) 1077, 1446
CaiI CAGNNNCTG 2 cut(s) 1178, 1283
CciI TCATGA 1 cut(s) 990
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 384, 740
ClaI ATCGAT 1 cut(s) 465
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 433
CviAII CATG 5 cut(s) 112, 439, 886, 991, 1305
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 326, 599, 1500
DpnI GATC 5 cut(s) 468, 746, 953, 980, 1422
DpnII GATC 5 cut(s) 466, 744, 951, 978, 1420
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
EciI GGCGGA 1 cut(s) 893
Ecl136II GAGCTC 2 cut(s) 262, 1254
Eco130I CCWWGG 3 cut(s) 111, 270, 438
Eco147I AGGCCT 1 cut(s) 1157
Eco24I GRGCYC 2 cut(s) 264, 1256
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 2 cut(s) 384, 740
Eco53kI GAGCTC 2 cut(s) 262, 1254
Eco57I CTGAAG 4 cut(s) 191, 1239, 1377, 1512
Eco81I CCTNAGG 1 cut(s) 1500
Eco88I CYCGRG 1 cut(s) 257
EcoICRI GAGCTC 2 cut(s) 262, 1254
EcoRII CCWGG 2 cut(s) 564, 870
EcoT14I CCWWGG 3 cut(s) 111, 270, 438
EcoT22I ATGCAT 1 cut(s) 1444
EcoT38I GRGCYC 2 cut(s) 264, 1256
ErhI CCWWGG 3 cut(s) 111, 270, 438
FaeI CATG 5 cut(s) 115, 442, 889, 994, 1308
FaqI GGGAC 1 cut(s) 370
FatI CATG 5 cut(s) 111, 438, 885, 990, 1304
FauI CCCGC 1 cut(s) 1054
FblI GTMKAC 2 cut(s) 1291, 1476
Fnu4HI GCNGC 9 cut(s) 158, 240, 323, 365, 675, 900, 903, 1179, 1281
FokI GGATG 3 cut(s) 138, 845, 893
FriOI GRGCYC 2 cut(s) 264, 1256
Fsp4HI GCNGC 9 cut(s) 158, 240, 323, 365, 675, 900, 903, 1179, 1281
FspBI CTAG 2 cut(s) 53, 161
GlaI GCGC 1 cut(s) 459
GluI GCNGC 9 cut(s) 158, 240, 323, 365, 675, 900, 903, 1179, 1281
HaeIII GGCC 5 cut(s) 116, 275, 378, 1157, 1362
HapII CCGG 3 cut(s) 291, 382, 670
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 5 cut(s) 115, 442, 889, 994, 1308
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 9 cut(s) 164, 509, 570, 641, 784, 1373, 1388, 1467, 1601
HpaII CCGG 3 cut(s) 291, 382, 670
HphI GGTGA 6 cut(s) 55, 301, 397, 817, 1290, 1562
Hpy166II GTNNAC 2 cut(s) 1292, 1477
Hpy8I GTNNAC 2 cut(s) 1292, 1477
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 5 cut(s) 44, 353, 1019, 1311, 1352
HpyCH4III ACNGT 2 cut(s) 17, 1296
HpyCH4IV ACGT 2 cut(s) 147, 477
HpyCH4V TGCA 8 cut(s) 367, 1070, 1172, 1193, 1398, 1442, 1556, 1586
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 361, 443, 680, 689, 896, 1067, 1178
HpyF3I CTNAG 3 cut(s) 326, 599, 1500
HpySE526I ACGT 2 cut(s) 147, 477
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 5 cut(s) 115, 442, 889, 994, 1308
HspAI GCGC 1 cut(s) 458
Kzo9I GATC 5 cut(s) 466, 744, 951, 978, 1420
LmnI GCTCC 5 cut(s) 31, 130, 360, 1102, 1251
Lsp1109I GCAGC 6 cut(s) 144, 309, 351, 661, 914, 1165
LweI GCATC 5 cut(s) 951, 1159, 1429, 1543, 1595
MaeI CTAG 2 cut(s) 53, 161
MaeII ACGT 2 cut(s) 147, 477
MaeIII GTNAC 4 cut(s) 74, 371, 403, 1296
MalI GATC 5 cut(s) 468, 746, 953, 980, 1422
MbiI CCGCTC 1 cut(s) 899
MboI GATC 5 cut(s) 466, 744, 951, 978, 1420
MboII GAAGA 5 cut(s) 184, 298, 484, 1232, 1423
MhlI GDGCHC 4 cut(s) 264, 1092, 1256, 1465
MlyI GAGTC 1 cut(s) 1367
MmeI TCCRAC 4 cut(s) 703, 987, 1077, 1365
Mph1103I ATGCAT 1 cut(s) 1444
MroXI GAANNNNTTC 3 cut(s) 594, 1486, 1600
MseI TTAA 6 cut(s) 531, 699, 734, 758, 1125, 1609
MslI CAYNNNNRTG 1 cut(s) 1303
MspA1I CMGCKG 3 cut(s) 157, 322, 1283
MspI CCGG 3 cut(s) 291, 382, 670
MspR9I CCNGG 3 cut(s) 566, 671, 872
MvaI CCWGG 2 cut(s) 566, 872
MwoI GCNNNNNNNGC 8 cut(s) 248, 361, 443, 680, 689, 896, 1067, 1178
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 2 cut(s) 111, 438
NdeII GATC 5 cut(s) 466, 744, 951, 978, 1420
NlaIII CATG 5 cut(s) 115, 442, 889, 994, 1308
NlaIV GGNNCC 4 cut(s) 27, 268, 386, 741
NmuCI GTSAC 2 cut(s) 403, 1296
NsiI ATGCAT 1 cut(s) 1444
PaeR7I CTCGAG 1 cut(s) 257
PagI TCATGA 1 cut(s) 990
PceI AGGCCT 1 cut(s) 1157
PcsI WCGNNNNNNNCGW 1 cut(s) 779
PdmI GAANNNNTTC 3 cut(s) 594, 1486, 1600
PfeI GAWTC 8 cut(s) 164, 509, 570, 641, 784, 1388, 1467, 1601
PkrI GCNGC 9 cut(s) 159, 241, 324, 366, 676, 901, 904, 1180, 1282
Ple19I CGATCG 2 cut(s) 469, 747
PleI GAGTC 1 cut(s) 1367
PpsI GAGTC 1 cut(s) 1367
PshAI GACNNNNGTC 1 cut(s) 300
Psp124BI GAGCTC 2 cut(s) 264, 1256
Psp6I CCWGG 2 cut(s) 564, 870
PspGI CCWGG 2 cut(s) 564, 870
PspN4I GGNNCC 4 cut(s) 27, 268, 386, 741
PspPI GGNCC 2 cut(s) 384, 740
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 2 cut(s) 1178, 1283
PvuI CGATCG 2 cut(s) 469, 747
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 1303
SacI GAGCTC 2 cut(s) 264, 1256
SaqAI TTAA 6 cut(s) 531, 699, 734, 758, 1125, 1609
SatI GCNGC 9 cut(s) 158, 240, 323, 365, 675, 900, 903, 1179, 1281
Sau3AI GATC 5 cut(s) 466, 744, 951, 978, 1420
Sau96I GGNCC 2 cut(s) 384, 740
SchI GAGTC 1 cut(s) 1367
ScrFI CCNGG 3 cut(s) 566, 671, 872
SduI GDGCHC 4 cut(s) 264, 1092, 1256, 1465
SfaNI GCATC 5 cut(s) 951, 1159, 1429, 1543, 1595
SfcI CTRYAG 2 cut(s) 191, 365
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 384, 740
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1303
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 1157
SsiI CCGC 9 cut(s) 155, 240, 397, 446, 878, 890, 899, 1061, 1281
SspI AATATT 1 cut(s) 1137
SspMI CTAG 2 cut(s) 53, 161
SstI GAGCTC 2 cut(s) 264, 1256
StuI AGGCCT 1 cut(s) 1157
StyD4I CCNGG 3 cut(s) 564, 669, 870
StyI CCWWGG 3 cut(s) 111, 270, 438
TaaI ACNGT 2 cut(s) 17, 1296
TaiI ACGT 2 cut(s) 150, 480
TauI GCSGC 3 cut(s) 242, 902, 1283
TfiI GAWTC 8 cut(s) 164, 509, 570, 641, 784, 1388, 1467, 1601
Tru1I TTAA 6 cut(s) 531, 699, 734, 758, 1125, 1609
Tru9I TTAA 6 cut(s) 531, 699, 734, 758, 1125, 1609
TscAI CASTG 2 cut(s) 1077, 1453
TseFI GTSAC 2 cut(s) 403, 1296
TseI GCWGC 6 cut(s) 157, 322, 364, 674, 902, 1178
Tsp45I GTSAC 2 cut(s) 403, 1296
TspDTI ATGAA 6 cut(s) 26, 140, 923, 1007, 1293, 1470
TspGWI ACGGA 3 cut(s) 140, 1239, 1494
TspRI CASTG 2 cut(s) 1077, 1453
VpaK11BI GGWCC 2 cut(s) 384, 740
XapI RAATTY 4 cut(s) 626, 701, 994, 1403
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 2 cut(s) 1291, 1476
XmnI GAANNNNTTC 3 cut(s) 594, 1486, 1600
XspI CTAG 2 cut(s) 53, 161
Zsp2I ATGCAT 1 cut(s) 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.