pycom15g13320
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012205
Physical Location & Seq
Forward (+)
9108503 .. 9111287
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2556 bp
ATGGTTAGGCCTGCTGATGCTGCTGACATGTACTGCATTACTGCTCTAACCGCGGCTTTAGCGTTGTTTGCTTGTCATGTTACACGCACGCATCTGTATGAACGCGATTGGTGGGTTCGGAAGTCAAAGATCGTAGGCGCCAAACGTACATCCATGCCTTCAACGGCAGTTAGGCACAAGCTTCTCTGTTTGATCGTTTTGTTGGAAGCGGTTTTATGCCAAGAAACTGCTCAAATAATGAAGGACAAGGCATCCTTGCTTTCTTTCAGAGAAGGGATTGTATCGGACCCCCACCGGGCTTTAGAGGACTGGAATTCCTTGGGTGTTCATGTTTGTAATTGGTCAGGCATCAAGTGTAACAAAGCTAGAGACTGCGTCGTGGAGCTTGATCTTAGTGGAAGGGCACTCGGAGGCACCATTTCCCCGTCTCTTGCTAATCTTTCTTCCTTACACATTCTTGATCTGTCAAGAAATTTTTTCAAAGGTCGCATTCCAGGTGAGTTAGGCACTCTTTCTCAACTTGGAGAACTCAGTTTATCTTCCAATCTTCTCGAGGGAAGCATTGCGGCTGAACTTGGCTTTCTTCGCAAATTAGTGTATCTCAATTTGGGAAGCAACCAGCTCACTGGTGAAATCCCAATGCCGCTTTTCTGCAATCATTCTTCGTCTTCTCTGCAGTACTTAGACCTCTCTAACAATTCTTTAAGCGGTGAAATTCCTTTGCCAGATGGATGTGAGCTTAAACACCTGAGGTTTCTTCTACTGTGGTCCAACCGTCTAGTTGGACAGGTTCCTGCAGCCCTTTCAAACTCCTCAAAACTTGAATGGCTCGATTTGGAGTCTAACATGCTGAGTGGGGAGTTGCCATCAGAGATTATACGAAAAATGCCACAACTGCAGTACCTCTACTTGTCCTACAATCGCTTTGTGAGCCATGACGGTAACACTAACCTTGAACCCTTTTTTGCCTCTCTGGTTAATGCCTCTAACTTTCAAGAGCTTGAATTAGCTGGAAACAATCTTGGGGGAGATTTACCTCCTATTATTGGTAATCTTTCTACCAATCTTGTACAACTTCACTTAGATACTAATCGTATTTACGGTTCAATTCCTCCTCATATTTCAAATCTTGTCAACCTCACCCTGTTGAATTTGTCTAGTAACCTTCTGAATGGAACCATCCCACCCAAACTGTGCCAAATGAAAAAGCTAGAGAGGGTGTTTTTGTCAAACAATTCACTCTCTGGTGAGATTCCATCTGCCTTTGGTGACATTCCCCATCTAGGCCTTCTAGATTTATCGAAAAACAAGCTTTCCGGTTCCATTCCAGATAGTTTTTCAAACCTTTCTCAGTTAAGAAGGCTCTTTCTTTATGAGAACCAACTCACGGGGACCATACCGCCAAGTCTAGGAAAGTGCATCAATTTAGAGATTCTAGACCTTTCTCACAACCAAATTTCAGGGGTGATTCCTAGCGAAGTTGCAGGCCTGAGGAGCTTGAAGTTGTACTTGAACCTGTCTAGCAATCACTTGCATGGACTGTTACCGATGGAGTTGAGCAAAATGGACATGGTATTAGCAGTTGACCTATCTTCCAACAATCTTTCCAGCACAATACCATCACAACTTGGGAGCTGCATTGCCCTCGAGTACCTCAACCTTTCGGTGAATTCCTTAGAAGGCCCTCTTCCGGATTCAATAGGAAAGCTACCTTATCTTCAAAAACTTGACGTATCCTCAAACCAACTGAATGCACAAATACCAGTGTCTCTGCAGGAATCATTAACTCTGAAGAAACTCAACTTCTCTTTCAACAACTTCTCCGGGAATGTATCAAACAATGGGGCCTTTTCTTTGCTGACCATAGACTCATTCCTAGGTAATGCTGGTCTCTGTGGATCAATAAAGGGCATGTCAAAATGCCGGAAGAAGCATAATCATTTGGCTATTCTGCCAATTATCCTGTCATTACTTATCACCCCCATATTTTGTGTATTCGGGTACCCCCTCATGCACAGGTCGAAATTCCAAAAACACTTGGCAGTTTTCGATCAAGACGATTCGGGGGATGATGAAGAAGAAGGGAAGAAAGAACACAAGTATCCGAGAATCTCATATGAGCAGCTCATTGAAGCCACGGATGGTTTCAGTGCTTCGAGCCTGATTGGTTCAGGCGGGTTTGGGCATGTCTACAGGGGTGTCCTTCAAGACAACTCAGTAATTGCTGTCAAGGTATTGGATTTGAAAACGGATGGAGATATTTTGGGAACCTTCAAAAGGGAATGCAGAGTTCTGAAGAGGACTAGGCACAGAAATTCGATCAGAATCATCACAGCGTGCAGTAGGCCGAACTTTAAGGCTCTTGTTCTGCCATTGATGTCAAATGGGAGCCTCGAGAGTCACCTATACCCGAGCCATGGACTGAAGGATCAGTTGAATTTGATTCAGTTGGTGAGCATCTGCAGTGATGTAGCTGAAGGAGTGGCCTATTTACACCATCACTCTCCTGTTAGAGTTGTGCACTGTGATCGATCAAACCAAGCAACATTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

852

Amino Acids

93.51

Weight (kDa)

7.34

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 82 - 120 2.4e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 224 - 310 2.7e-06 Leucine-rich repeat region
LRR_8 PF13855 249 - 309 1.3e-08 Leucine rich repeat
LRR_8 PF13855 403 - 462 3.4e-09 Leucine rich repeat
LRR_4 PF12799 451 - 487 6.6e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 473 - 511 3.7e-06 Leucine rich repeat
LRR_8 PF13855 528 - 583 1.3e-07 Leucine rich repeat
Pkinase PF00069 718 - 843 1.2e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 720 - 841 5.5e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24130 AT2G24130
fragaria_vesca FvH4_1g00610
malus_domestica MD02G1004900.v1.1 MD15G1148900.v1.1
prunus_persica Prupe.7G266100_v2.0.a1
pyrus_communis pycom02g00360 pycom15g13320
rosa_chinensis RchiOBHm_Chr2g0085231
rosa_laevigata RLG00000015669
rosa_multiflora Rmu_sc0008510.1_g000003 Rmu_sc0011989.1_g000008
rosa_roxburghii Rroxscaffold_2G00155540
rosa_rugosa Rorug01G0458400
rosa_samantha Rh2AG007100 Rh2BG007700 Rh2CG008500 Rh2DG008800
rosa_wichuraiana Rw2G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2001
AccB1I GGYRCC 3 cut(s) 137, 413, 2001
AccI GTMKAC 1 cut(s) 2190
AccII CGCG 2 cut(s) 53, 105
AccIII TCCGGA 1 cut(s) 1690
AciI CCGC 8 cut(s) 51, 53, 209, 566, 644, 708, 1400, 2175
AclWI GGATC 2 cut(s) 1906, 2436
AcsI RAATTY 9 cut(s) 313, 472, 714, 1150, 1455, 1669, 2024, 2314, 2437
AcuI CTGAAG 4 cut(s) 1811, 2315, 2444, 2496
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 2337
AfaI GTAC 8 cut(s) 32, 148, 680, 902, 1071, 1508, 1652, 2003
AfiI CCNNNNNNNGG 8 cut(s) 295, 1046, 1283, 1387, 1409, 1690, 2277, 2417
AflIII ACRYGT 1 cut(s) 27
AjnI CCWGG 1 cut(s) 493
Alw21I GWGCWC 1 cut(s) 2523
Alw26I GTCTC 4 cut(s) 363, 432, 1773, 1895
Alw44I GTGCAC 1 cut(s) 2519
AlwI GGATC 2 cut(s) 1906, 2436
Ama87I CYCGRG 4 cut(s) 551, 1646, 2393, 2410
Aor13HI TCCGGA 1 cut(s) 1690
AoxI GGCC 7 cut(s) 8, 1285, 1486, 1681, 1845, 2345, 2484
ApaLI GTGCAC 1 cut(s) 2519
ApeKI GCWGC 4 cut(s) 20, 797, 1635, 2122
ApoI RAATTY 9 cut(s) 313, 472, 714, 1150, 1455, 1669, 2024, 2314, 2437
Asp718I GGTACC 1 cut(s) 2001
AspA2I CCTAGG 1 cut(s) 1876
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 5 cut(s) 286, 768, 1392, 1682, 1845
AsuC2I CCSGG 2 cut(s) 296, 1825
AvaI CYCGRG 4 cut(s) 551, 1646, 2393, 2410
AvaII GGWCC 3 cut(s) 286, 768, 1392
AvrII CCTAGG 1 cut(s) 1876
AxyI CCTNAGG 2 cut(s) 749, 1490
BaeGI GKGCMC 2 cut(s) 406, 2523
BaeI ACNNNNGTAYC 4 cut(s) 884, 917, 2084, 2117
BanI GGYRCC 3 cut(s) 137, 413, 2001
BbsI GAAGAC 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 2523
BbvI GCAGC 4 cut(s) 7, 809, 1622, 2134
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 4 cut(s) 1627, 1661, 2510, 2544
BciT130I CCWGG 1 cut(s) 495
BciVI GTATCC 2 cut(s) 1744, 2112
BcnI CCSGG 2 cut(s) 296, 1825
BcoDI GTCTC 4 cut(s) 363, 432, 1773, 1895
BfmI CTRYAG 6 cut(s) 674, 795, 896, 1772, 2191, 2461
BfoI RGCGCY 1 cut(s) 141
BfuI GTATCC 2 cut(s) 1744, 2112
BisI GCNGC 7 cut(s) 21, 54, 567, 644, 798, 1636, 2123
BlnI CCTAGG 1 cut(s) 1876
BlsI GCNGC 7 cut(s) 22, 55, 568, 645, 799, 1637, 2124
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 3 cut(s) 296, 495, 1825
Bme18I GGWCC 3 cut(s) 286, 768, 1392
BmeT110I CYCGRG 4 cut(s) 551, 1646, 2393, 2410
BmgT120I GGNCC 5 cut(s) 286, 768, 1392, 1682, 1845
BmrFI CCNGG 3 cut(s) 296, 495, 1825
BmsI GCATC 6 cut(s) 7, 100, 260, 357, 1428, 2466
BpiI GAAGAC 1 cut(s) 660
BpuMI CCSGG 2 cut(s) 296, 1825
Bsa29I ATCGAT 1 cut(s) 2530
BsaBI GATNNNNATC 2 cut(s) 1089, 2324
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 1895
BsaJI CCNNGG 5 cut(s) 51, 318, 1876, 2136, 2416
BsaWI WCCGGW 2 cut(s) 1316, 1690
BsaXI ACNNNNNCTCC 2 cut(s) 1805, 1835
Bsc4I CCNNNNNNNGG 8 cut(s) 295, 1046, 1283, 1387, 1409, 1690, 2277, 2417
Bse1I ACTGG 3 cut(s) 314, 631, 1763
Bse21I CCTNAGG 2 cut(s) 749, 1490
Bse3DI GCAATG 2 cut(s) 561, 1638
Bse8I GATNNNNATC 2 cut(s) 1089, 2324
BseAI TCCGGA 1 cut(s) 1690
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 2530
BseDI CCNNGG 5 cut(s) 51, 318, 1876, 2136, 2416
BseGI GGATG 7 cut(s) 149, 251, 737, 1179, 2074, 2146, 2257
BseJI GATNNNNATC 2 cut(s) 1089, 2324
BseLI CCNNNNNNNGG 8 cut(s) 295, 1046, 1283, 1387, 1409, 1690, 2277, 2417
BseMI GCAATG 2 cut(s) 561, 1638
BseMII CTCAG 6 cut(s) 544, 740, 842, 1364, 1481, 2229
BseNI ACTGG 3 cut(s) 314, 631, 1763
BseRI GAGGAG 3 cut(s) 802, 1104, 1507
BseSI GKGCMC 2 cut(s) 406, 2523
BseXI GCAGC 4 cut(s) 7, 809, 1622, 2134
BsgI GTGCAG 1 cut(s) 2359
Bsh1236I CGCG 2 cut(s) 53, 105
BshFI GGCC 7 cut(s) 10, 1287, 1488, 1683, 1847, 2347, 2486
BshNI GGYRCC 3 cut(s) 137, 413, 2001
BshVI ATCGAT 1 cut(s) 2530
BsiHKAI GWGCWC 1 cut(s) 2523
BsiHKCI CYCGRG 4 cut(s) 551, 1646, 2393, 2410
BsiSI CCGG 5 cut(s) 295, 1317, 1691, 1824, 1924
BslFI GGGAC 1 cut(s) 1405
BslI CCNNNNNNNGG 8 cut(s) 295, 1046, 1283, 1387, 1409, 1690, 2277, 2417
BsmAI GTCTC 4 cut(s) 363, 432, 1773, 1895
BsmBI CGTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 1405
BsmI GAATGC 3 cut(s) 489, 1756, 2288
BsnI GGCC 7 cut(s) 10, 1287, 1488, 1683, 1847, 2347, 2486
Bso31I GGTCTC 1 cut(s) 1895
BsoBI CYCGRG 4 cut(s) 551, 1646, 2393, 2410
Bsp1286I GDGCHC 2 cut(s) 406, 2523
Bsp13I TCCGGA 1 cut(s) 1690
Bsp1407I TGTACA 1 cut(s) 1069
Bsp19I CCATGG 1 cut(s) 2416
BspACI CCGC 8 cut(s) 51, 53, 209, 566, 644, 708, 1400, 2175
BspANI GGCC 7 cut(s) 10, 1287, 1488, 1683, 1847, 2347, 2486
BspCNI CTCAG 6 cut(s) 543, 741, 843, 1363, 1482, 2228
BspDI ATCGAT 1 cut(s) 2530
BspEI TCCGGA 1 cut(s) 1690
BspFNI CGCG 2 cut(s) 53, 105
BspMAI CTGCAG 5 cut(s) 678, 799, 900, 1776, 2465
BspPI GGATC 2 cut(s) 1906, 2436
BspT107I GGYRCC 3 cut(s) 137, 413, 2001
BspTNI GGTCTC 1 cut(s) 1895
BsrDI GCAATG 2 cut(s) 561, 1638
BsrGI TGTACA 1 cut(s) 1069
BsrI ACTGG 3 cut(s) 314, 631, 1763
BssECI CCNNGG 5 cut(s) 51, 318, 1876, 2136, 2416
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 3 cut(s) 318, 1876, 2416
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 7 cut(s) 765, 776, 941, 1103, 1194, 1542, 2525
Bst6I CTCTTC 2 cut(s) 1692, 2291
BstACI GRCGYC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 1069
BstC8I GCNNGC 4 cut(s) 12, 89, 1486, 2338
BstDSI CCRYGG 3 cut(s) 51, 2136, 2416
BstENI CCTNNNNNAGG 1 cut(s) 2275
BstF5I GGATG 7 cut(s) 149, 251, 737, 1179, 2074, 2146, 2257
BstFNI CGCG 2 cut(s) 53, 105
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstMAI GTCTC 4 cut(s) 363, 432, 1773, 1895
BstMWI GCNNNNNNNGC 8 cut(s) 20, 50, 59, 68, 585, 895, 930, 1494
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 4 cut(s) 31, 850, 1915, 2189
BstSCI CCNGG 3 cut(s) 294, 493, 1823
BstSFI CTRYAG 6 cut(s) 674, 795, 896, 1772, 2191, 2461
BstSLI GKGCMC 2 cut(s) 406, 2523
BstUI CGCG 2 cut(s) 53, 105
BstV1I GCAGC 4 cut(s) 7, 809, 1622, 2134
BstV2I GAAGAC 1 cut(s) 660
BstXI CCANNNNNNTGG 1 cut(s) 626
Bsu15I ATCGAT 1 cut(s) 2530
Bsu36I CCTNAGG 2 cut(s) 749, 1490
BsuI GTATCC 2 cut(s) 1744, 2112
BsuRI GGCC 7 cut(s) 10, 1287, 1488, 1683, 1847, 2347, 2486
BsuTUI ATCGAT 1 cut(s) 2530
BtgI CCRYGG 3 cut(s) 51, 2136, 2416
BtsCI GGATG 7 cut(s) 149, 251, 737, 1179, 2074, 2146, 2257
BtsI GCAGTG 1 cut(s) 2470
BtsIMutI CAGTG 5 cut(s) 624, 1770, 2155, 2470, 2521
Cac8I GCNNGC 4 cut(s) 12, 89, 1486, 2338
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 5 cut(s) 286, 768, 1392, 1682, 1845
Cfr42I CCGCGG 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 2530
CseI GACGC 1 cut(s) 364
Csp6I GTAC 8 cut(s) 31, 147, 679, 901, 1070, 1507, 1651, 2002
CviQI GTAC 8 cut(s) 31, 147, 679, 901, 1070, 1507, 1651, 2002
DinI GGCGCC 1 cut(s) 139
DraIII CACNNNGTG 1 cut(s) 2337
Eam1104I CTCTTC 2 cut(s) 1692, 2291
EarI CTCTTC 2 cut(s) 1692, 2291
Eco130I CCWWGG 3 cut(s) 318, 1876, 2416
Eco147I AGGCCT 3 cut(s) 10, 1287, 1488
Eco31I GGTCTC 1 cut(s) 1895
Eco47I GGWCC 3 cut(s) 286, 768, 1392
Eco57I CTGAAG 4 cut(s) 1811, 2315, 2444, 2496
Eco81I CCTNAGG 2 cut(s) 749, 1490
Eco88I CYCGRG 4 cut(s) 551, 1646, 2393, 2410
EcoNI CCTNNNNNAGG 1 cut(s) 2275
EcoO109I RGGNCCY 2 cut(s) 1682, 1845
EcoRI GAATTC 2 cut(s) 313, 1669
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 3 cut(s) 318, 1876, 2416
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 3 cut(s) 318, 1876, 2416
Esp3I CGTCTC 1 cut(s) 432
FalI AAGNNNNNCTT 6 cut(s) 239, 271, 1493, 1525, 1671, 1703
FaqI GGGAC 1 cut(s) 1405
FauI CCCGC 1 cut(s) 2168
FauNDI CATATG 1 cut(s) 2116
FblI GTMKAC 1 cut(s) 2190
Fnu4HI GCNGC 7 cut(s) 21, 54, 567, 644, 798, 1636, 2123
FokI GGATG 7 cut(s) 136, 238, 744, 1166, 2081, 2153, 2264
Fsp4HI GCNGC 7 cut(s) 21, 54, 567, 644, 798, 1636, 2123
GlaI GCGC 1 cut(s) 139
GluI GCNGC 7 cut(s) 21, 54, 567, 644, 798, 1636, 2123
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 7 cut(s) 10, 1287, 1488, 1683, 1847, 2347, 2486
HapII CCGG 5 cut(s) 295, 1317, 1691, 1824, 1924
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 2 cut(s) 1135, 1585
HindII GTYRAC 2 cut(s) 1135, 1585
HindIII AAGCTT 2 cut(s) 179, 1310
HpaII CCGG 5 cut(s) 295, 1317, 1691, 1824, 1924
Hpy166II GTNNAC 4 cut(s) 1135, 1585, 2191, 2521
Hpy8I GTNNAC 4 cut(s) 1135, 1585, 2191, 2521
Hpy99I CGWCG 1 cut(s) 380
HpyCH4III ACNGT 7 cut(s) 765, 776, 941, 1103, 1194, 1542, 2525
HpyCH4IV ACGT 2 cut(s) 145, 1731
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 50, 59, 68, 585, 895, 930, 1494
HpySE526I ACGT 2 cut(s) 145, 1731
Hsp92I GRCGYC 1 cut(s) 138
HspAI GCGC 1 cut(s) 138
KasI GGCGCC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 1690
KpnI GGTACC 1 cut(s) 2005
KspI CCGCGG 1 cut(s) 54
LmnI GCTCC 4 cut(s) 382, 1494, 1632, 2388
Lsp1109I GCAGC 4 cut(s) 7, 809, 1622, 2134
LweI GCATC 6 cut(s) 7, 100, 260, 357, 1428, 2466
MaeII ACGT 2 cut(s) 145, 1731
MaeIII GTNAC 7 cut(s) 79, 356, 941, 1160, 1268, 1542, 2399
MhlI GDGCHC 2 cut(s) 406, 2523
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 3 cut(s) 848, 1862, 2407
MmeI TCCRAC 4 cut(s) 183, 763, 795, 1620
MroI TCCGGA 1 cut(s) 1690
MseI TTAA 6 cut(s) 704, 741, 978, 1355, 1784, 2355
MslI CAYNNNNRTG 2 cut(s) 96, 1533
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 5 cut(s) 295, 1317, 1691, 1824, 1924
MspR9I CCNGG 3 cut(s) 296, 495, 1825
Mva1269I GAATGC 3 cut(s) 489, 1756, 2288
MvaI CCWGG 1 cut(s) 495
MvnI CGCG 2 cut(s) 53, 105
MwoI GCNNNNNNNGC 8 cut(s) 20, 50, 59, 68, 585, 895, 930, 1494
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 2 cut(s) 296, 1825
NcoI CCATGG 1 cut(s) 2416
NdeI CATATG 1 cut(s) 2116
NmuCI GTSAC 2 cut(s) 1268, 2399
NspI RCATGY 4 cut(s) 31, 850, 1915, 2189
PaeR7I CTCGAG 3 cut(s) 551, 1646, 2393
PceI AGGCCT 3 cut(s) 10, 1287, 1488
PciI ACATGT 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 2055
PctI GAATGC 3 cut(s) 489, 1756, 2288
PfeI GAWTC 9 cut(s) 1252, 1432, 1468, 1694, 1778, 2060, 2109, 2325, 2443
PflFI GACNNNGTC 1 cut(s) 374
PfoI TCCNGGA 1 cut(s) 1823
PkrI GCNGC 7 cut(s) 22, 55, 568, 645, 799, 1637, 2124
PleI GAGTC 3 cut(s) 847, 1862, 2406
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 3 cut(s) 847, 1862, 2406
PscI ACATGT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspPI GGNCC 5 cut(s) 286, 768, 1392, 1682, 1845
PspXI VCTCGAGB 1 cut(s) 1646
PsrI GAACNNNNNNTAC 2 cut(s) 1087, 1119
PstI CTGCAG 5 cut(s) 678, 799, 900, 1776, 2465
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 8 cut(s) 32, 148, 680, 902, 1071, 1508, 1652, 2003
RsaNI GTAC 8 cut(s) 31, 147, 679, 901, 1070, 1507, 1651, 2002
RseI CAYNNNNRTG 2 cut(s) 96, 1533
SacII CCGCGG 1 cut(s) 54
SaqAI TTAA 6 cut(s) 704, 741, 978, 1355, 1784, 2355
SatI GCNGC 7 cut(s) 21, 54, 567, 644, 798, 1636, 2123
Sau96I GGNCC 5 cut(s) 286, 768, 1392, 1682, 1845
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 3 cut(s) 848, 1862, 2407
ScrFI CCNGG 3 cut(s) 296, 495, 1825
SduI GDGCHC 2 cut(s) 406, 2523
SfaNI GCATC 6 cut(s) 7, 100, 260, 357, 1428, 2466
SfcI CTRYAG 6 cut(s) 674, 795, 896, 1772, 2191, 2461
SfoI GGCGCC 1 cut(s) 139
Sfr274I CTCGAG 3 cut(s) 551, 1646, 2393
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
SinI GGWCC 3 cut(s) 286, 768, 1392
SlaI CTCGAG 3 cut(s) 551, 1646, 2393
SmiMI CAYNNNNRTG 2 cut(s) 96, 1533
SmlI CTYRAG 3 cut(s) 551, 1646, 2393
SmoI CTYRAG 3 cut(s) 551, 1646, 2393
SseBI AGGCCT 3 cut(s) 10, 1287, 1488
SsiI CCGC 8 cut(s) 51, 53, 209, 566, 644, 708, 1400, 2175
SspDI GGCGCC 1 cut(s) 137
StuI AGGCCT 3 cut(s) 10, 1287, 1488
StyD4I CCNGG 3 cut(s) 294, 493, 1823
StyI CCWWGG 3 cut(s) 318, 1876, 2416
TaaI ACNGT 7 cut(s) 765, 776, 941, 1103, 1194, 1542, 2525
TaiI ACGT 2 cut(s) 148, 1734
TatI WGTACW 4 cut(s) 30, 678, 1069, 1506
TauI GCSGC 3 cut(s) 56, 569, 646
TfiI GAWTC 9 cut(s) 1252, 1432, 1468, 1694, 1778, 2060, 2109, 2325, 2443
Tru1I TTAA 6 cut(s) 704, 741, 978, 1355, 1784, 2355
Tru9I TTAA 6 cut(s) 704, 741, 978, 1355, 1784, 2355
TscAI CASTG 5 cut(s) 631, 1770, 2155, 2470, 2528
TseFI GTSAC 2 cut(s) 1268, 2399
TseI GCWGC 4 cut(s) 20, 797, 1635, 2122
Tsp45I GTSAC 2 cut(s) 1268, 2399
TspDTI ATGAA 5 cut(s) 114, 254, 317, 1217, 2088
TspGWI ACGGA 2 cut(s) 2153, 2264
TspRI CASTG 5 cut(s) 631, 1770, 2155, 2470, 2528
Tth111I GACNNNGTC 1 cut(s) 374
VneI GTGCAC 1 cut(s) 2519
VpaK11BI GGWCC 3 cut(s) 286, 768, 1392
XagI CCTNNNNNAGG 1 cut(s) 2275
XapI RAATTY 9 cut(s) 313, 472, 714, 1150, 1455, 1669, 2024, 2314, 2437
XbaI TCTAGA 2 cut(s) 1291, 1435
XceI RCATGY 4 cut(s) 31, 850, 1915, 2189
XhoI CTCGAG 3 cut(s) 551, 1646, 2393
XmaJI CCTAGG 1 cut(s) 1876
XmiI GTMKAC 1 cut(s) 2190
ZrmI AGTACT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.