pycom15g14590

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012555
Physical Location & Seq
Forward (+)
9975483 .. 9977776
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2196 bp
ATGATGGGGAAGCAAAGTGGAAAAAAGAATAAAAACGCAGGAGAATATTCAGGTGATAGTTCGAATCAAAGCAAAATCGGGGATACTAGCCCAAAAGCCTATGATAAGGACACAGAAGTTTTCATTTCGATGTCTCAAGAACTGAAGGACGAGGGGAACAAGTTGTTTCAGAAGAGGGATGTTGAAGGAGCTTTGTTGAAATATGAAAAAGCCCTCAAATTGCTTCCGAGGAATCACATCGATGTATCCTATCTTAGGAGCAACATGGCAGCGTGCTATATGCAGATGGGGCTGAGTGAGTACCCGAGGGCTATCAATGAATGTAATTTGGCGCTTGAAGTCACCCCTAAGTACAGTAAGGCACTGTTGAAGAGAGCAAAGTGTTACGAGGCTTTGAATAGGTTGGATTTGGCTTTGAGAGATGTTGGTACCGTTTTGAACATGGAGCCAAAAAATGTCATGGCAATGGAGGTCGCAGAACGAGTGAAAAGCGTACTTGAGGAGAAGGGGTTGAGGGTGAATGACACGGTAATTGAGTTGCCTGCAGATTATGTTGAACCAACTCATGCTTCGCAGCCAGGGAAAATAATGAAGTTGAAGAGTCGGAAGAAGAAGAAGGGCAACAAAGATAATGAGAAGAAGGCCGAGGAAAACATTGAGGAGAAGGCTGAGGACAAGATTGAGGAAGAGAAGGCTGTGGATGTAGCTGAGGAAGAGAAGTCTGTGGAAATAACTGAAGACAAGAAGGCGGTGGAAATAGCTGAGGAGAGGAAGGCAGAGGTCAAGGTAGTTGTCGAGGAGGCGATCAGCAGTTCAAATGAAGAAGTGCCTAAAAGAAGTGTGAAATTGGTATTTGGAGAGGACATAAGGTGGGCTGAGTTGCCGCTAAATTGCACCCTCCTGCAACTGAGAGAGGTTGTGCATGATCGGTTTCCTAGCTCAAGAGCAGTTCTCATCAAATATAGAGACCAAGAAGGTGATTTGGTCACAATCACTTCCAATGAAGAGTTGCGATGGGCTGAAGGATCAGCAGAATCGGAAGGGTCTGTCAGACTTTATGTAGTGGAAGTTAACCCCGAGCAGGATCCCTTCTTCGAGAAACTGGAAATCGAACCGCACAAGCAACATAGTGTAGCTGAGAATGGTAGTGTGGTAAAACACAAGGATACAAAGAGACCACCTTGCATTGAGGATTGGGTAATCAATTTTGCTCAGCTGTTCAAGAACTATGCTGGGATTGAATCTGATGCATACTTGGATCTTCATGAACTCGGGGTGAAGCTTTATTCCGAGGCAATGGAGGAGACAGTCACAAGTGAAGAAGCGCAAGACCTGTTTGACATAGCAGGGGAGAAGTTTCAAGAAATGGGAGCTTTAGCGCTGTTCAACTGGGGAAATGTTCACATGGCCAGGGCGAGAAAGAAGGTGTATTTCACCGAAGATTCTTCGAAAGAATCTATTATTTCAAATATCAAAAGTGCATATGATTGGGCACAGAAGGAATACATAGAAGCAGGAAGGAGATACGAAGAAGCGCTGCAGATCAAACCGGACTTTTATGAAGGCTATCTGGCCCTCGGGCAGCAGCAGTTTGAACAGGCAAAACTTTCTTGGTATTATGCAATTAGCAGCAATGTTGATCTGGAGACGTGGCCTTCCACTGAGGTTCTGCGACTTTATAACTACGCCGAAGACAATATGGAAAAAGGCATGCAGCTGTTCGAGGAATTGGAAGAGCAACGCCTGAGAGAACTTTCCGGTCCAAGTAGTGTTAAAACTCAGGTGCAGCAAATGGGTTTGAACGGAATTTGCAAAGACGTATCAGCAAATGAGGCTGCAGACCAGGCTACAAGCATGAGATGTCAGATAAATCTCCTATGGGGCACCATGCTGTACGAGCGATCTATTGTGGAATTCAAAGTAGGGCTACCCATATGGAAAGAATGTTTGGAAGTTGCAGTTGAAAAATTTGCACTTGCTGGGGCTTCACCTACAGATATAGCAGTCATGCTCAAAAATCACTCTTCAAATGATAATGCGTTGGAAGGTCTGGGGTTCAAAATTGACGAGATCGTACAAGCATGGAACGAGATGCACGAAGCGAAAAAGTGGCAGACTGGAATTCCATCATTCCGGTTAGAGCCATTACTACGAAGACGAGTTTCCAGAATTTATTCTGCCTTGGAGCACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

732

Amino Acids

83.25

Weight (kDa)

5.12

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 280 - 356 3.4e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1680
Acc65I GGTACC 1 cut(s) 428
AccB1I GGYRCC 2 cut(s) 428, 1883
AciI CCGC 3 cut(s) 749, 884, 1115
AclWI GGATC 4 cut(s) 1033, 1079, 1092, 1266
AcoI YGGCCR 1 cut(s) 1407
AcsI RAATTY 5 cut(s) 1806, 1913, 1967, 2121, 2169
AcuI CTGAAG 3 cut(s) 164, 756, 1041
AfaI GTAC 6 cut(s) 302, 353, 430, 495, 1895, 2076
AfeI AGCGCT 2 cut(s) 1380, 1536
AfiI CCNNNNNNNGG 2 cut(s) 255, 1081
AjiI CACGTC 1 cut(s) 1650
AjnI CCWGG 3 cut(s) 577, 1409, 1842
AjuI GAANNNNNNNTTGG 4 cut(s) 553, 585, 1931, 1963
AluBI AGCT 9 cut(s) 191, 707, 761, 939, 1136, 1216, 1282, 1373, 1717
AluI AGCT 9 cut(s) 191, 707, 761, 939, 1136, 1216, 1282, 1373, 1717
Alw21I GWGCWC 1 cut(s) 2190
Alw26I GTCTC 5 cut(s) 138, 960, 1168, 1298, 1640
AlwI GGATC 4 cut(s) 1033, 1079, 1092, 1266
Ama87I CYCGRG 4 cut(s) 304, 1076, 1271, 1577
Aor51HI AGCGCT 2 cut(s) 1380, 1536
AoxI GGCC 4 cut(s) 642, 1407, 1572, 1652
ApeKI GCWGC 9 cut(s) 269, 574, 1537, 1582, 1585, 1629, 1714, 1786, 1835
ApoI RAATTY 5 cut(s) 1806, 1913, 1967, 2121, 2169
Asp700I GAANNNNTTC 1 cut(s) 2173
Asp718I GGTACC 1 cut(s) 428
AspLEI GCGC 4 cut(s) 334, 1327, 1381, 1537
AspS9I GGNCC 2 cut(s) 1573, 1760
AsuHPI GGTGA 7 cut(s) 65, 334, 529, 989, 1288, 1426, 1980
AsuII TTCGAA 2 cut(s) 62, 1448
AvaI CYCGRG 4 cut(s) 304, 1076, 1271, 1577
AvaII GGWCC 1 cut(s) 1760
BaeGI GKGCMC 2 cut(s) 1495, 1886
BalI TGGCCA 1 cut(s) 1409
BamHI GGATCC 1 cut(s) 1084
BanI GGYRCC 2 cut(s) 428, 1883
BbsI GAAGAC 3 cut(s) 744, 1698, 2161
Bbv12I GWGCWC 1 cut(s) 2190
BbvCI CCTCAGC 3 cut(s) 669, 708, 762
BbvI GCAGC 9 cut(s) 281, 586, 1524, 1594, 1597, 1641, 1726, 1798, 1822
BccI CCATC 3 cut(s) 280, 1008, 2134
BciT130I CCWGG 3 cut(s) 579, 1411, 1844
BciVI GTATCC 3 cut(s) 76, 256, 1159
BcoDI GTCTC 5 cut(s) 138, 960, 1168, 1298, 1640
BfaI CTAG 2 cut(s) 87, 936
BfmI CTRYAG 4 cut(s) 543, 1538, 1836, 1992
BfoI RGCGCY 3 cut(s) 335, 1382, 1538
BfuI GTATCC 3 cut(s) 76, 256, 1159
BlpI GCTNAGC 1 cut(s) 1212
Bme1390I CCNGG 3 cut(s) 579, 1411, 1844
Bme18I GGWCC 1 cut(s) 1760
BmeT110I CYCGRG 4 cut(s) 304, 1076, 1271, 1577
BmgBI CACGTC 1 cut(s) 1650
BmgT120I GGNCC 2 cut(s) 1573, 1760
BmiI GGNNCC 4 cut(s) 430, 447, 1086, 1885
BmrFI CCNGG 3 cut(s) 579, 1411, 1844
BmrI ACTGGG 1 cut(s) 1399
BmsI GCATC 2 cut(s) 1237, 2082
BmuI ACTGGG 1 cut(s) 1399
BpiI GAAGAC 3 cut(s) 744, 1698, 2161
BplI GAGNNNNNCTC 2 cut(s) 936, 968
BpmI CTGGAG 1 cut(s) 1664
Bpu10I CCTNAGC 3 cut(s) 669, 708, 762
Bpu1102I GCTNAGC 1 cut(s) 1212
Bpu14I TTCGAA 2 cut(s) 62, 1448
BpuEI CTTGAG 3 cut(s) 120, 518, 925
Bsa29I ATCGAT 1 cut(s) 240
BsaI GGTCTC 2 cut(s) 960, 1168
BsaJI CCNNGG 8 cut(s) 227, 305, 578, 645, 1290, 1410, 1576, 2181
BsaWI WCCGGW 3 cut(s) 1549, 1757, 2133
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 1081
Bse1I ACTGG 3 cut(s) 1107, 1394, 2122
Bse3DI GCAATG 3 cut(s) 471, 1302, 1639
BseBI CCWGG 3 cut(s) 579, 1411, 1844
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 8 cut(s) 227, 305, 578, 645, 1290, 1410, 1576, 2181
BseGI GGATG 2 cut(s) 184, 706
BseLI CCNNNNNNNGG 2 cut(s) 255, 1081
BseMI GCAATG 3 cut(s) 471, 1302, 1639
BseNI ACTGG 3 cut(s) 1107, 1394, 2122
BseRI GAGGAG 5 cut(s) 515, 674, 779, 812, 1316
BseSI GKGCMC 2 cut(s) 1495, 1886
BseXI GCAGC 9 cut(s) 281, 586, 1524, 1594, 1597, 1641, 1726, 1798, 1822
BseYI CCCAGC 2 cut(s) 1232, 1979
BsgI GTGCAG 1 cut(s) 1805
BshFI GGCC 4 cut(s) 644, 1409, 1574, 1654
BshNI GGYRCC 2 cut(s) 428, 1883
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 2190
BsiHKCI CYCGRG 4 cut(s) 304, 1076, 1271, 1577
BsiSI CCGG 3 cut(s) 1550, 1758, 2134
BslI CCNNNNNNNGG 2 cut(s) 255, 1081
BsmAI GTCTC 5 cut(s) 138, 960, 1168, 1298, 1640
BsmBI CGTCTC 1 cut(s) 1640
BsnI GGCC 4 cut(s) 644, 1409, 1574, 1654
Bso31I GGTCTC 2 cut(s) 960, 1168
BsoBI CYCGRG 4 cut(s) 304, 1076, 1271, 1577
Bsp119I TTCGAA 2 cut(s) 62, 1448
Bsp1286I GDGCHC 3 cut(s) 1495, 1886, 2190
Bsp143I GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
Bsp1720I GCTNAGC 1 cut(s) 1212
BspACI CCGC 3 cut(s) 749, 884, 1115
BspANI GGCC 4 cut(s) 644, 1409, 1574, 1654
BspDI ATCGAT 1 cut(s) 240
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 4 cut(s) 430, 447, 1086, 1885
BspMAI CTGCAG 3 cut(s) 547, 1542, 1840
BspPI GGATC 4 cut(s) 1033, 1079, 1092, 1266
BspQI GCTCTTC 1 cut(s) 1728
BspT104I TTCGAA 2 cut(s) 62, 1448
BspT107I GGYRCC 2 cut(s) 428, 1883
BspTNI GGTCTC 2 cut(s) 960, 1168
BsrDI GCAATG 3 cut(s) 471, 1302, 1639
BsrI ACTGG 3 cut(s) 1107, 1394, 2122
BssECI CCNNGG 8 cut(s) 227, 305, 578, 645, 1290, 1410, 1576, 2181
BssMI GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
BssT1I CCWWGG 1 cut(s) 2181
Bst2UI CCWGG 3 cut(s) 579, 1411, 1844
Bst4CI ACNGT 5 cut(s) 356, 366, 433, 529, 1309
Bst6I CTCTTC 8 cut(s) 167, 365, 593, 681, 708, 999, 1728, 2029
BstBI TTCGAA 2 cut(s) 62, 1448
BstC8I GCNNGC 4 cut(s) 274, 543, 1712, 2190
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 2 cut(s) 184, 706
BstH2I RGCGCY 3 cut(s) 335, 1382, 1538
BstHHI GCGC 4 cut(s) 334, 1327, 1381, 1537
BstKTI GATC 9 cut(s) 807, 928, 1028, 1087, 1261, 1545, 1642, 1904, 2073
BstMAI GTCTC 5 cut(s) 138, 960, 1168, 1298, 1640
BstMBI GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
BstMWI GCNNNNNNNGC 4 cut(s) 289, 1832, 1844, 1897
BstNI CCWGG 3 cut(s) 579, 1411, 1844
BstNSI RCATGY 1 cut(s) 1714
BstSCI CCNGG 3 cut(s) 577, 1409, 1842
BstSFI CTRYAG 4 cut(s) 543, 1538, 1836, 1992
BstSLI GKGCMC 2 cut(s) 1495, 1886
BstV1I GCAGC 9 cut(s) 281, 586, 1524, 1594, 1597, 1641, 1726, 1798, 1822
BstV2I GAAGAC 3 cut(s) 744, 1698, 2161
BstX2I RGATCY 2 cut(s) 1084, 1258
BstYI RGATCY 2 cut(s) 1084, 1258
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 3 cut(s) 76, 256, 1159
BsuRI GGCC 4 cut(s) 644, 1409, 1574, 1654
BsuTUI ATCGAT 1 cut(s) 240
BtgZI GCGATG 1 cut(s) 1027
BtrI CACGTC 1 cut(s) 1650
BtsCI GGATG 2 cut(s) 184, 706
BtsIMutI CAGTG 2 cut(s) 362, 1659
Cac8I GCNNGC 4 cut(s) 274, 543, 1712, 2190
CciI TCATGA 1 cut(s) 1264
CfoI GCGC 4 cut(s) 334, 1327, 1381, 1537
Cfr13I GGNCC 2 cut(s) 1573, 1760
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 6 cut(s) 301, 352, 429, 494, 1894, 2075
CviQI GTAC 6 cut(s) 301, 352, 429, 494, 1894, 2075
DpnI GATC 9 cut(s) 806, 927, 1027, 1086, 1260, 1544, 1641, 1903, 2072
DpnII GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
EaeI YGGCCR 1 cut(s) 1407
Eam1104I CTCTTC 8 cut(s) 167, 365, 593, 681, 708, 999, 1728, 2029
EarI CTCTTC 8 cut(s) 167, 365, 593, 681, 708, 999, 1728, 2029
Eco130I CCWWGG 1 cut(s) 2181
Eco31I GGTCTC 2 cut(s) 960, 1168
Eco47I GGWCC 1 cut(s) 1760
Eco47III AGCGCT 2 cut(s) 1380, 1536
Eco57I CTGAAG 3 cut(s) 164, 756, 1041
Eco88I CYCGRG 4 cut(s) 304, 1076, 1271, 1577
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoRI GAATTC 2 cut(s) 1913, 2121
EcoRII CCWGG 3 cut(s) 577, 1409, 1842
EcoT14I CCWWGG 1 cut(s) 2181
EcoT22I ATGCAT 1 cut(s) 1252
ErhI CCWWGG 1 cut(s) 2181
Esp3I CGTCTC 1 cut(s) 1640
FauNDI CATATG 2 cut(s) 1483, 1934
FokI GGATG 2 cut(s) 191, 713
FspBI CTAG 2 cut(s) 87, 936
GlaI GCGC 4 cut(s) 333, 1326, 1380, 1536
GsaI CCCAGC 2 cut(s) 1236, 1983
GsuI CTGGAG 1 cut(s) 1664
HaeII RGCGCY 3 cut(s) 335, 1382, 1538
HaeIII GGCC 4 cut(s) 644, 1409, 1574, 1654
HapII CCGG 3 cut(s) 1550, 1758, 2134
HhaI GCGC 4 cut(s) 334, 1327, 1381, 1537
Hin6I GCGC 4 cut(s) 332, 1325, 1379, 1535
HinP1I GCGC 4 cut(s) 332, 1325, 1379, 1535
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HindIII AAGCTT 1 cut(s) 1280
HinfI GANTC 7 cut(s) 64, 232, 601, 1034, 1241, 1442, 1454
HpaI GTTAAC 1 cut(s) 1072
HpaII CCGG 3 cut(s) 1550, 1758, 2134
HphI GGTGA 7 cut(s) 65, 334, 529, 989, 1288, 1426, 1980
Hpy166II GTNNAC 2 cut(s) 1072, 1402
Hpy188I TCNGA 8 cut(s) 171, 228, 606, 1039, 1052, 1246, 1291, 1866
Hpy188III TCNNGA 8 cut(s) 137, 942, 1096, 1222, 1265, 1361, 1643, 2166
Hpy8I GTNNAC 2 cut(s) 1072, 1402
HpyCH4III ACNGT 5 cut(s) 356, 366, 433, 529, 1309
HpyCH4IV ACGT 2 cut(s) 1649, 1818
HpyF10VI GCNNNNNNNGC 4 cut(s) 289, 1832, 1844, 1897
HpySE526I ACGT 2 cut(s) 1649, 1818
HspAI GCGC 4 cut(s) 332, 1325, 1379, 1535
KpnI GGTACC 1 cut(s) 432
KspAI GTTAAC 1 cut(s) 1072
Kzo9I GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
LguI GCTCTTC 1 cut(s) 1728
LmnI GCTCC 5 cut(s) 188, 258, 445, 1370, 2185
Lsp1109I GCAGC 9 cut(s) 281, 586, 1524, 1594, 1597, 1641, 1726, 1798, 1822
LweI GCATC 2 cut(s) 1237, 2082
MaeI CTAG 2 cut(s) 87, 936
MaeII ACGT 2 cut(s) 1649, 1818
MaeIII GTNAC 4 cut(s) 340, 383, 985, 1309
MalI GATC 9 cut(s) 806, 927, 1027, 1086, 1260, 1544, 1641, 1903, 2072
MboI GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
MflI RGATCY 2 cut(s) 1084, 1258
MhlI GDGCHC 3 cut(s) 1495, 1886, 2190
MlsI TGGCCA 1 cut(s) 1409
MluNI TGGCCA 1 cut(s) 1409
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 3 cut(s) 384, 584, 2022
Mox20I TGGCCA 1 cut(s) 1409
Mph1103I ATGCAT 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 2173
MscI TGGCCA 1 cut(s) 1409
MseI TTAA 2 cut(s) 1071, 1773
MslI CAYNNNNRTG 3 cut(s) 128, 240, 464
Msp20I TGGCCA 1 cut(s) 1409
MspA1I CMGCKG 2 cut(s) 1216, 1717
MspI CCGG 3 cut(s) 1550, 1758, 2134
MspR9I CCNGG 3 cut(s) 579, 1411, 1844
MvaI CCWGG 3 cut(s) 579, 1411, 1844
MwoI GCNNNNNNNGC 4 cut(s) 289, 1832, 1844, 1897
NdeI CATATG 2 cut(s) 1483, 1934
NdeII GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
NlaIV GGNNCC 4 cut(s) 430, 447, 1086, 1885
NmeAIII GCCGAG 1 cut(s) 670
NmuCI GTSAC 3 cut(s) 340, 985, 1309
NsiI ATGCAT 1 cut(s) 1252
NspI RCATGY 1 cut(s) 1714
NspV TTCGAA 2 cut(s) 62, 1448
PaeI GCATGC 1 cut(s) 1714
PagI TCATGA 1 cut(s) 1264
PciSI GCTCTTC 1 cut(s) 1728
PcsI WCGNNNNNNNCGW 1 cut(s) 2094
PdmI GAANNNNTTC 1 cut(s) 2173
PfeI GAWTC 6 cut(s) 64, 232, 1034, 1241, 1442, 1454
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
PsiI TTATAA 1 cut(s) 1680
Psp6I CCWGG 3 cut(s) 577, 1409, 1842
PspFI CCCAGC 2 cut(s) 1232, 1979
PspGI CCWGG 3 cut(s) 577, 1409, 1842
PspN4I GGNNCC 4 cut(s) 430, 447, 1086, 1885
PspPI GGNCC 2 cut(s) 1573, 1760
PstI CTGCAG 3 cut(s) 547, 1542, 1840
PsuI RGATCY 2 cut(s) 1084, 1258
PvuII CAGCTG 2 cut(s) 1216, 1717
RsaI GTAC 6 cut(s) 302, 353, 430, 495, 1895, 2076
RsaNI GTAC 6 cut(s) 301, 352, 429, 494, 1894, 2075
RseI CAYNNNNRTG 3 cut(s) 128, 240, 464
SapI GCTCTTC 1 cut(s) 1728
SaqAI TTAA 2 cut(s) 1071, 1773
Sau3AI GATC 9 cut(s) 804, 925, 1025, 1084, 1258, 1542, 1639, 1901, 2070
Sau96I GGNCC 2 cut(s) 1573, 1760
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 3 cut(s) 579, 1411, 1844
SduI GDGCHC 3 cut(s) 1495, 1886, 2190
SfaNI GCATC 2 cut(s) 1237, 2082
SfcI CTRYAG 4 cut(s) 543, 1538, 1836, 1992
SfuI TTCGAA 2 cut(s) 62, 1448
SinI GGWCC 1 cut(s) 1760
SmiMI CAYNNNNRTG 3 cut(s) 128, 240, 464
SmlI CTYRAG 3 cut(s) 135, 497, 940
SmoI CTYRAG 3 cut(s) 135, 497, 940
SphI GCATGC 1 cut(s) 1714
SsiI CCGC 3 cut(s) 749, 884, 1115
SspI AATATT 1 cut(s) 47
SspMI CTAG 2 cut(s) 87, 936
StyD4I CCNGG 3 cut(s) 577, 1409, 1842
StyI CCWWGG 1 cut(s) 2181
TaaI ACNGT 5 cut(s) 356, 366, 433, 529, 1309
TaiI ACGT 2 cut(s) 1652, 1821
TaqI TCGA 8 cut(s) 62, 128, 240, 795, 1095, 1110, 1448, 1722
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 886
TfiI GAWTC 6 cut(s) 64, 232, 1034, 1241, 1442, 1454
Tru1I TTAA 2 cut(s) 1071, 1773
Tru9I TTAA 2 cut(s) 1071, 1773
TscAI CASTG 2 cut(s) 369, 1666
TseFI GTSAC 3 cut(s) 340, 985, 1309
TseI GCWGC 9 cut(s) 269, 574, 1537, 1582, 1585, 1629, 1714, 1786, 1835
Tsp45I GTSAC 3 cut(s) 340, 985, 1309
TspDTI ATGAA 9 cut(s) 112, 219, 333, 605, 834, 1017, 1253, 1281, 1575
TspGWI ACGGA 1 cut(s) 1818
TspRI CASTG 2 cut(s) 369, 1666
VpaK11BI GGWCC 1 cut(s) 1760
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 5 cut(s) 1806, 1913, 1967, 2121, 2169
XceI RCATGY 1 cut(s) 1714
XmnI GAANNNNTTC 1 cut(s) 2173
XspI CTAG 2 cut(s) 87, 936
Zsp2I ATGCAT 1 cut(s) 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.