Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

15130377 .. 15134941

Gene Structure 4565 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGCGTGATGCCATGAACAATCTGAAGGGGGATTCTAACAAGATTAACCCGTTGGTCCCAGTAGATCTTGTCATTGATCACTCGGTTCAAGTAGATATTGCAAGATCAGAAAATGCAGTGCAGGCAAATATGGAACTTGAGTTCCAGCGAAACAAAGAACGGTTTGGTTTTCTGAAATGGGGATCAAATGCTTTCCATAACATGCTCGTTGTTCCTCCTGGATCAGGGATTGTTCATCAGGTTAATTTGGAATACCTTGGTCGAGTTGTGTTCAACACAGACGGCTTGCTTTATCCTGATAGTGTTGTTGGGACAGATTCACACACAACTATGATAGACGGATTAGGTGTTGCTGGCTGGGGTGTGGGTGGTATCGAAGCAGAAGCCACTATGCTTGGCCAGCCCATGAGCATGGTCCTGCCTGGTGTTGTTGGGTTTAAATTATTAGGAAAACTGAGAGACGGTGTGACAGCTACCGACTTGGTTCTGACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGCAAGTTTGTGGAGTTCTATGGGGAAGGCATGAGTGAATTGTCACTAGCAGACCGTGCCACTATTGCTAACATGTCTCCTGAGTATGGTGCAACCATGGGCTTCTTCCCTGTGGATCATGTCACCTTGCAATATCTAAAACTTACTGGCAGAAGTGATGACAAGGTCGCTCTGATAGAATCCTATTTACGGGCTAATAAGATGTTTGTTGACTACAATGAGCCCCAGATCGAGAGAGTGTACTCGTCGTATCTTGAGCTAAATCTTAATGAAGTGGAACCATGCATATCAGGTCCAAAGAGGCCACATGATCGTGTTGCATTGAAAGAAATGAAAGTGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTGCTGTGCCAAAAGAATCTCAAAGTAAAGTCATAGAATTCGATTTTCACGGAACCCCAGCACAGCTTAGGCATGGGGATGTTGTTATTGCAGCTATCACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTTGGAGCTGCTTTGGTTGCAAAGAAAGCTTGTGATTTAGGATTGGAGGTCAAGCCATGGATTAAGACAAGTCTTGCTCCCGGTTCTGGGGTTGTAACCAAATACTTGCAAAAAAGTGGGTTGCAGAAGTATTTGAATCAGTTAGGGTTTCATATTGTTGGGTATGGATGCACCACTTGCATTGGGAATTCAGGAGATATTGATGATGCAGTAGCGGCTGCAATTACGGAAAATGATGTTGTAGCTGCGGCTGTATTATCTGGAAATAGGAATTTTGAGGGTCGTGTGCATCCATTAACAAGGGCTAATTATCTTGCTTCTCCTCCCCTTGTTGTTGCCTATGCACTTGCTGGAACGGTAGATATTGATTTCGAAACAGAACCAATTGGGGTGGGAAAAGATGGGAAAAAGATATTTTTCAGGGACATCTGGCCATCCAATGAAGAAGTAGCAACGGTTGTGCAATCAAATGTGCTTCCTCATATGTTTATGGCTACCTATGAAGCAATCACCAAAGGAAATCCAATGTGGAATCAATTATCTGTACCATCTGGCACTCTTTATGCCTGGGACCCAAAATCAACTTATATACATGAACCGCCGTATTTTAAGGATATGACCATGTCTCCCCCAGGCGCTCATGGAGTGAAGAATGCTTACTGTTTGCTCAACTTTGGAGATAGTATTACAACCGATCACATCTCACCAGCTGGTAGTATTCACAAAGACAGCCCAGCAGCCAAATACCTACTGGAACGTGGCGTTGATAGAAGAGACTTTAACTCCTATGGAAGTCGCCGTGGTAATGATGAGGTTATGGCAAGAGGCACTTTTGCAAATATTCGGCTGGTCAATAAGTTTTTGAAAGGAGAAGTTGGTCCCAAAACAATCCACATTCCCACTGGAGAGAAACTGTCCGTGTTTGATGCTGCTATGAGGTACAAAAGCGAAGGACATGATACAATTATTTTGGCTGGTGCCGAGTATGGAAGTGGAAGTTCTCGTGATTGGGCTGCAAAGGGTCCCATGCTACTGGGTGTGAAGGCGGTCATAGCAAAGAGCTTTGAGCGCATTCACCGTAGTAATCTCGTTGGCATGGGCATAATTCCACTATGTTTCAAGCCTGGGGAGGATGCCGACTCTCTTGGTTTAACCGGGGAGGAACGCTACACCATCGATCTTCCAAGTAGTGTTAGTGAAATTAGACCCGGTCAGGATATCACAGTGGTGACAGATAACGGCAAGTCCTTCGTATGTACACTACGTTTCGATACAGAGGTTGAGCTGGCCTATTTCGACCACGGGGGCATTTTGCAGTATGTCATCAGGAATCTGATCAAAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

796

Amino Acids

86.74

Weight (kDa)

5.99

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	1 - 465	5.1e-157	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	594 - 721	2e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2018
AciI	CCGC	4 cut(s)	1256, 1289, 1640, 2087
AclWI	GGATC	3 cut(s)	190, 229, 648
AcoI	YGGCCR	2 cut(s)	397, 1472
AcsI	RAATTY	3 cut(s)	944, 1228, 1312
AcuI	CTGAAG	1 cut(s)	44
AfaI	GTAC	4 cut(s)	767, 1587, 1982, 2299
AfiI	CCNNNNNNNGG	5 cut(s)	224, 611, 714, 1127, 1128
AflIII	ACRYGT	1 cut(s)	597
AgsI	TTSAA	6 cut(s)	89, 274, 850, 1177, 1906, 2161
AjnI	CCWGG	5 cut(s)	217, 421, 1607, 1672, 2164
AleI	CACNNNNGTG	1 cut(s)	2267
AloI	GAACNNNNNNTCC	4 cut(s)	125, 126, 157, 158
Alw26I	GTCTC	4 cut(s)	453, 606, 1671, 1809
AlwI	GGATC	3 cut(s)	190, 229, 648
AoxI	GGCC	4 cut(s)	397, 827, 1472, 2328
ApeKI	GCWGC	7 cut(s)	998, 1049, 1259, 1286, 1778, 1970, 2054
ApoI	RAATTY	3 cut(s)	944, 1228, 1312
AspLEI	GCGC	2 cut(s)	1679, 2112
AspS9I	GGNCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
AsuC2I	CCSGG	3 cut(s)	1122, 2197, 2250
AsuHPI	GGTGA	6 cut(s)	640, 997, 1543, 1737, 2108, 2281
AsuII	TTCGAA	1 cut(s)	1413
AvaII	GGWCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
BaeI	ACNNNNGTAYC	2 cut(s)	2304, 2337
BalI	TGGCCA	2 cut(s)	399, 1474
BanI	GGYRCC	1 cut(s)	2018
BanII	GRGCYC	1 cut(s)	750
BarI	GAAGNNNNNNTAC	2 cut(s)	1682, 1714
BauI	CACGAG	1 cut(s)	2043
BbvCI	CCTCAGC	1 cut(s)	506
BbvI	GCAGC	7 cut(s)	1010, 1036, 1246, 1273, 1790, 1957, 2041
BccI	CCATC	4 cut(s)	1436, 1483, 1597, 2222
BceAI	ACGGC	4 cut(s)	298, 1627, 1824, 2296
BcgI	CGANNNNNNTGC	2 cut(s)	2272, 2306
BciT130I	CCWGG	5 cut(s)	219, 423, 1609, 1674, 2166
BclI	TGATCA	2 cut(s)	76, 2376
BcnI	CCSGG	3 cut(s)	1122, 2197, 2250
BcoDI	GTCTC	4 cut(s)	453, 606, 1671, 1809
BfaI	CTAG	2 cut(s)	572, 1032
BfoI	RGCGCY	1 cut(s)	1680
BglII	AGATCT	1 cut(s)	64
BisI	GCNGC	9 cut(s)	999, 1050, 1257, 1260, 1287, 1290, 1779, 1971, 2055
BlsI	GCNGC	9 cut(s)	1000, 1051, 1258, 1261, 1288, 1291, 1780, 1972, 2056
Bme1390I	CCNGG	8 cut(s)	219, 423, 1122, 1609, 1674, 2166, 2197, 2250
Bme18I	GGWCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
BmgT120I	GGNCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
BmiI	GGNNCC	9 cut(s)	57, 804, 961, 1613, 1614, 1921, 2020, 2064, 2065
BmrFI	CCNGG	8 cut(s)	219, 423, 1122, 1609, 1674, 2166, 2197, 2250
BmrI	ACTGGG	2 cut(s)	53, 2084
BmsI	GCATC	5 cut(s)	1199, 1237, 1339, 1957, 2164
BmuI	ACTGGG	2 cut(s)	53, 2084
BpmI	CTGGAG	1 cut(s)	1965
Bpu10I	CCTNAGC	2 cut(s)	506, 974
Bpu14I	TTCGAA	1 cut(s)	1413
BpuEI	CTTGAG	2 cut(s)	158, 800
BpuMI	CCSGG	3 cut(s)	1122, 2197, 2250
Bsa29I	ATCGAT	1 cut(s)	2217
BsaJI	CCNNGG	9 cut(s)	256, 621, 1097, 1608, 1672, 1840, 2165, 2196, 2341
BsaXI	ACNNNNNCTCC	4 cut(s)	1651, 1681, 1808, 1838
Bsc4I	CCNNNNNNNGG	5 cut(s)	224, 611, 714, 1127, 1128
Bse1I	ACTGG	6 cut(s)	59, 676, 1008, 1797, 1948, 2079
BseBI	CCWGG	5 cut(s)	219, 423, 1609, 1674, 2166
BseCI	ATCGAT	1 cut(s)	2217
BseDI	CCNNGG	9 cut(s)	256, 621, 1097, 1608, 1672, 1840, 2165, 2196, 2341
BseGI	GGATG	5 cut(s)	991, 1214, 1330, 1475, 2179
BseLI	CCNNNNNNNGG	5 cut(s)	224, 611, 714, 1127, 1128
BseMII	CTCAG	3 cut(s)	446, 497, 597
BseNI	ACTGG	6 cut(s)	59, 676, 1008, 1797, 1948, 2079
BseRI	GAGGAG	1 cut(s)	1353
BseXI	GCAGC	7 cut(s)	1010, 1036, 1246, 1273, 1790, 1957, 2041
BseYI	CCCAGC	3 cut(s)	357, 964, 1774
BsgI	GTGCAG	1 cut(s)	140
BshFI	GGCC	4 cut(s)	399, 829, 1474, 2330
BshNI	GGYRCC	1 cut(s)	2018
BshVI	ATCGAT	1 cut(s)	2217
BsiSI	CCGG	3 cut(s)	1122, 2196, 2250
BslFI	GGGAC	6 cut(s)	41, 325, 1478, 1625, 1905, 2049
BslI	CCNNNNNNNGG	5 cut(s)	224, 611, 714, 1127, 1128
BsmAI	GTCTC	4 cut(s)	453, 606, 1671, 1809
BsmBI	CGTCTC	1 cut(s)	453
BsmFI	GGGAC	6 cut(s)	41, 325, 1478, 1625, 1905, 2049
BsmI	GAATGC	2 cut(s)	1699, 2112
BsnI	GGCC	4 cut(s)	399, 829, 1474, 2330
Bsp119I	TTCGAA	1 cut(s)	1413
Bsp1286I	GDGCHC	1 cut(s)	750
Bsp1407I	TGTACA	1 cut(s)	2297
Bsp19I	CCATGG	2 cut(s)	621, 1097
BspACI	CCGC	4 cut(s)	1256, 1289, 1640, 2087
BspANI	GGCC	4 cut(s)	399, 829, 1474, 2330
BspCNI	CTCAG	3 cut(s)	447, 498, 598
BspDI	ATCGAT	1 cut(s)	2217
BspLI	GGNNCC	9 cut(s)	57, 804, 961, 1613, 1614, 1921, 2020, 2064, 2065
BspPI	GGATC	3 cut(s)	190, 229, 648
BspT104I	TTCGAA	1 cut(s)	1413
BspT107I	GGYRCC	1 cut(s)	2018
BsrGI	TGTACA	1 cut(s)	2297
BsrI	ACTGG	6 cut(s)	59, 676, 1008, 1797, 1948, 2079
BssECI	CCNNGG	9 cut(s)	256, 621, 1097, 1608, 1672, 1840, 2165, 2196, 2341
BssSI	CACGAG	1 cut(s)	2043
BssT1I	CCWWGG	3 cut(s)	256, 621, 1097
Bst2BI	CACGAG	1 cut(s)	2043
Bst2UI	CCWGG	5 cut(s)	219, 423, 1609, 1674, 2166
Bst6I	CTCTTC	1 cut(s)	1807
BstAPI	GCANNNNNTGC	2 cut(s)	581, 1218
BstAUI	TGTACA	1 cut(s)	2297
BstBI	TTCGAA	1 cut(s)	1413
BstC8I	GCNNGC	7 cut(s)	123, 287, 355, 401, 873, 877, 2328
BstDEI	CTNAG	4 cut(s)	455, 506, 606, 974
BstDSI	CCRYGG	4 cut(s)	621, 1097, 1840, 2341
BstENI	CCTNNNNNAGG	1 cut(s)	222
BstF5I	GGATG	5 cut(s)	991, 1214, 1330, 1475, 2179
BstH2I	RGCGCY	1 cut(s)	1680
BstHHI	GCGC	2 cut(s)	1679, 2112
BstMAI	GTCTC	4 cut(s)	453, 606, 1671, 1809
BstNI	CCWGG	5 cut(s)	219, 423, 1609, 1674, 2166
BstNSI	RCATGY	4 cut(s)	205, 601, 875, 879
BstSCI	CCNGG	8 cut(s)	217, 421, 1120, 1607, 1672, 2164, 2195, 2248
BstV1I	GCAGC	7 cut(s)	1010, 1036, 1246, 1273, 1790, 1957, 2041
BstX2I	RGATCY	1 cut(s)	64
BstXI	CCANNNNNNTGG	2 cut(s)	412, 2074
BstYI	RGATCY	1 cut(s)	64
Bsu15I	ATCGAT	1 cut(s)	2217
BsuRI	GGCC	4 cut(s)	399, 829, 1474, 2330
BsuTUI	ATCGAT	1 cut(s)	2217
BtgI	CCRYGG	4 cut(s)	621, 1097, 1840, 2341
BtsCI	GGATG	5 cut(s)	991, 1214, 1330, 1475, 2179
BtsI	GCAGTG	1 cut(s)	123
BtsIMutI	CAGTG	3 cut(s)	123, 1941, 2271
Cac8I	GCNNGC	7 cut(s)	123, 287, 355, 401, 873, 877, 2328
CfoI	GCGC	2 cut(s)	1679, 2112
Cfr13I	GGNCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
ClaI	ATCGAT	1 cut(s)	2217
Csp6I	GTAC	4 cut(s)	766, 1586, 1981, 2298
CspCI	CAANNNNNGTGG	6 cut(s)	376, 411, 1204, 1239, 1413, 1448
CviQI	GTAC	4 cut(s)	766, 1586, 1981, 2298
DdeI	CTNAG	4 cut(s)	455, 506, 606, 974
DraI	TTTAAA	1 cut(s)	439
EaeI	YGGCCR	2 cut(s)	397, 1472
Eam1104I	CTCTTC	1 cut(s)	1807
EarI	CTCTTC	1 cut(s)	1807
Eco130I	CCWWGG	3 cut(s)	256, 621, 1097
Eco24I	GRGCYC	1 cut(s)	750
Eco32I	GATATC	1 cut(s)	2260
Eco47I	GGWCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
Eco57I	CTGAAG	1 cut(s)	44
EcoNI	CCTNNNNNAGG	1 cut(s)	222
EcoO109I	RGGNCCY	2 cut(s)	1612, 2063
EcoRI	GAATTC	2 cut(s)	944, 1228
EcoRII	CCWGG	5 cut(s)	217, 421, 1607, 1672, 2164
EcoRV	GATATC	1 cut(s)	2260
EcoT14I	CCWWGG	3 cut(s)	256, 621, 1097
EcoT22I	ATGCAT	2 cut(s)	812, 877
EcoT38I	GRGCYC	1 cut(s)	750
ErhI	CCWWGG	3 cut(s)	256, 621, 1097
Esp3I	CGTCTC	1 cut(s)	453
FalI	AAGNNNNNCTT	2 cut(s)	1855, 1887
FaqI	GGGAC	6 cut(s)	41, 325, 1478, 1625, 1905, 2049
FauNDI	CATATG	1 cut(s)	1524
FbaI	TGATCA	2 cut(s)	76, 2376
Fnu4HI	GCNGC	9 cut(s)	999, 1050, 1257, 1260, 1287, 1290, 1779, 1971, 2055
FokI	GGATG	5 cut(s)	998, 1221, 1317, 1462, 2186
FriOI	GRGCYC	1 cut(s)	750
Fsp4HI	GCNGC	9 cut(s)	999, 1050, 1257, 1260, 1287, 1290, 1779, 1971, 2055
FspBI	CTAG	2 cut(s)	572, 1032
GlaI	GCGC	2 cut(s)	1678, 2111
GluI	GCNGC	9 cut(s)	999, 1050, 1257, 1260, 1287, 1290, 1779, 1971, 2055
GsaI	CCCAGC	3 cut(s)	361, 968, 1778
GsuI	CTGGAG	1 cut(s)	1965
HaeII	RGCGCY	1 cut(s)	1680
HaeIII	GGCC	4 cut(s)	399, 829, 1474, 2330
HapII	CCGG	3 cut(s)	1122, 2196, 2250
HhaI	GCGC	2 cut(s)	1679, 2112
Hin6I	GCGC	2 cut(s)	1677, 2110
HinP1I	GCGC	2 cut(s)	1677, 2110
HincII	GTYRAC	1 cut(s)	736
HindII	GTYRAC	1 cut(s)	736
HindIII	AAGCTT	1 cut(s)	1068
HinfI	GANTC	8 cut(s)	32, 317, 704, 923, 1177, 1573, 2180, 2371
HpaII	CCGG	3 cut(s)	1122, 2196, 2250
HphI	GGTGA	6 cut(s)	640, 997, 1543, 1737, 2108, 2281
Hpy166II	GTNNAC	3 cut(s)	736, 766, 2300
Hpy188I	TCNGA	6 cut(s)	24, 109, 174, 489, 699, 2376
Hpy188III	TCNNGA	9 cut(s)	296, 605, 757, 779, 1233, 1302, 2045, 2255, 2368
Hpy8I	GTNNAC	3 cut(s)	736, 766, 2300
Hpy99I	CGWCG	1 cut(s)	775
HpyAV	CCTTC	5 cut(s)	19, 545, 1985, 2077, 2299
HpyCH4IV	ACGT	2 cut(s)	1798, 2305
HpyF3I	CTNAG	4 cut(s)	455, 506, 606, 974
HpySE526I	ACGT	2 cut(s)	1798, 2305
HspAI	GCGC	2 cut(s)	1677, 2110
KflI	GGGWCCC	2 cut(s)	1612, 2063
Ksp22I	TGATCA	2 cut(s)	76, 2376
LmnI	GCTCC	2 cut(s)	1046, 1123
Lsp1109I	GCAGC	7 cut(s)	1010, 1036, 1246, 1273, 1790, 1957, 2041
LweI	GCATC	5 cut(s)	1199, 1237, 1339, 1957, 2164
MaeI	CTAG	2 cut(s)	572, 1032
MaeII	ACGT	2 cut(s)	1798, 2305
MaeIII	GTNAC	6 cut(s)	466, 493, 567, 646, 1135, 2269
MboII	GAAGA	5 cut(s)	622, 1496, 1702, 1824, 2213
MfeI	CAATTG	1 cut(s)	1425
MflI	RGATCY	1 cut(s)	64
MhlI	GDGCHC	1 cut(s)	750
MlsI	TGGCCA	2 cut(s)	399, 1474
MluNI	TGGCCA	2 cut(s)	399, 1474
MlyI	GAGTC	1 cut(s)	2174
MmeI	TCCRAC	1 cut(s)	874
Mox20I	TGGCCA	2 cut(s)	399, 1474
Mph1103I	ATGCAT	2 cut(s)	812, 877
MscI	TGGCCA	2 cut(s)	399, 1474
MslI	CAYNNNNRTG	5 cut(s)	329, 410, 837, 984, 2267
Msp20I	TGGCCA	2 cut(s)	399, 1474
MspA1I	CMGCKG	1 cut(s)	1751
MspI	CCGG	3 cut(s)	1122, 2196, 2250
MspR9I	CCNGG	8 cut(s)	219, 423, 1122, 1609, 1674, 2166, 2197, 2250
MunI	CAATTG	1 cut(s)	1425
Mva1269I	GAATGC	2 cut(s)	1699, 2112
MvaI	CCWGG	5 cut(s)	219, 423, 1609, 1674, 2166
NciI	CCSGG	3 cut(s)	1122, 2197, 2250
NcoI	CCATGG	2 cut(s)	621, 1097
NdeI	CATATG	1 cut(s)	1524
NlaIV	GGNNCC	9 cut(s)	57, 804, 961, 1613, 1614, 1921, 2020, 2064, 2065
NmeAIII	GCCGAG	1 cut(s)	2047
NmuCI	GTSAC	4 cut(s)	466, 567, 646, 2269
NsiI	ATGCAT	2 cut(s)	812, 877
NspI	RCATGY	4 cut(s)	205, 601, 875, 879
NspV	TTCGAA	1 cut(s)	1413
OliI	CACNNNNGTG	1 cut(s)	2267
PaeI	GCATGC	2 cut(s)	875, 879
PciI	ACATGT	1 cut(s)	597
PctI	GAATGC	2 cut(s)	1699, 2112
PfeI	GAWTC	7 cut(s)	32, 317, 704, 923, 1177, 1573, 2371
PflFI	GACNNNGTC	3 cut(s)	689, 1663, 2250
PfoI	TCCNGGA	1 cut(s)	217
PkrI	GCNGC	9 cut(s)	1000, 1051, 1258, 1261, 1288, 1291, 1780, 1972, 2056
PleI	GAGTC	1 cut(s)	2174
PpsI	GAGTC	1 cut(s)	2174
PpuMI	RGGWCCY	2 cut(s)	1612, 2063
PscI	ACATGT	1 cut(s)	597
Psp5II	RGGWCCY	2 cut(s)	1612, 2063
Psp6I	CCWGG	5 cut(s)	217, 421, 1607, 1672, 2164
PspFI	CCCAGC	3 cut(s)	357, 964, 1774
PspGI	CCWGG	5 cut(s)	217, 421, 1607, 1672, 2164
PspN4I	GGNNCC	9 cut(s)	57, 804, 961, 1613, 1614, 1921, 2020, 2064, 2065
PspPI	GGNCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
PspPPI	RGGWCCY	2 cut(s)	1612, 2063
PsuI	RGATCY	1 cut(s)	64
PsyI	GACNNNGTC	3 cut(s)	689, 1663, 2250
PvuII	CAGCTG	1 cut(s)	1751
RsaI	GTAC	4 cut(s)	767, 1587, 1982, 2299
RsaNI	GTAC	4 cut(s)	766, 1586, 1981, 2298
RseI	CAYNNNNRTG	5 cut(s)	329, 410, 837, 984, 2267
SatI	GCNGC	9 cut(s)	999, 1050, 1257, 1260, 1287, 1290, 1779, 1971, 2055
Sau96I	GGNCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
SchI	GAGTC	1 cut(s)	2174
ScrFI	CCNGG	8 cut(s)	219, 423, 1122, 1609, 1674, 2166, 2197, 2250
SduI	GDGCHC	1 cut(s)	750
SfaNI	GCATC	5 cut(s)	1199, 1237, 1339, 1957, 2164
SfuI	TTCGAA	1 cut(s)	1413
SinI	GGWCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
SmiMI	CAYNNNNRTG	5 cut(s)	329, 410, 837, 984, 2267
SmlI	CTYRAG	2 cut(s)	137, 779
SmoI	CTYRAG	2 cut(s)	137, 779
SphI	GCATGC	2 cut(s)	875, 879
SsiI	CCGC	4 cut(s)	1256, 1289, 1640, 2087
SspI	AATATT	1 cut(s)	1882
SspMI	CTAG	2 cut(s)	572, 1032
StyD4I	CCNGG	8 cut(s)	217, 421, 1120, 1607, 1672, 2164, 2195, 2248
StyI	CCWWGG	3 cut(s)	256, 621, 1097
TaiI	ACGT	2 cut(s)	1801, 2308
TaqI	TCGA	8 cut(s)	262, 375, 756, 948, 1413, 2217, 2310, 2337
TatI	WGTACW	2 cut(s)	765, 2297
TauI	GCSGC	2 cut(s)	1259, 1292
TfiI	GAWTC	7 cut(s)	32, 317, 704, 923, 1177, 1573, 2371
TscAI	CASTG	3 cut(s)	123, 1948, 2271
TseFI	GTSAC	4 cut(s)	466, 567, 646, 2269
TseI	GCWGC	7 cut(s)	998, 1049, 1259, 1286, 1778, 1970, 2054
Tsp45I	GTSAC	4 cut(s)	466, 567, 646, 2269
TspDTI	ATGAA	8 cut(s)	29, 224, 810, 872, 1181, 1497, 1557, 1650
TspGWI	ACGGA	4 cut(s)	354, 972, 1283, 1948
TspRI	CASTG	3 cut(s)	123, 1948, 2271
Tth111I	GACNNNGTC	3 cut(s)	689, 1663, 2250
VpaK11BI	GGWCC	6 cut(s)	55, 415, 818, 1612, 1919, 2063
XagI	CCTNNNNNAGG	1 cut(s)	222
XapI	RAATTY	3 cut(s)	944, 1228, 1312
XceI	RCATGY	4 cut(s)	205, 601, 875, 879
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1789, 1940
XspI	CTAG	2 cut(s)	572, 1032
Zsp2I	ATGCAT	2 cut(s)	812, 877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.