pycom15g21190

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015059
Physical Location & Seq
Forward (+)
15190093 .. 15192520
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2382 bp
ATGAACGACACTCTCTCTCCTCCGCCGTCCCCCGGCGACGACGCCGATACCTACGAGGCCTGGTACGGGAACATCCAGTACCTTCTCAACATCTCCGCCATCGGCTCCTTCTTCTGCGTCTTCTTCTTCATCTTCGTCAAGCTCCGCAGCGACCACCGCCGCATGCCCGGCCCATCCGCCCTCGTCGCCAAGCTCCTTGCTGTGTGGCACGCTACCTGTCGTGAGATTGCTCGCCACTGCGGCGCTGACGCCGCCCAATTTCTCTTGATTGAGGGTGGAAGCTGTGGGTTGCTGTTGTCCATGGCGGTTTTGGCTGTGCTTGTGATGCTTCCGCTGAATCTCTACGCCGGGAGTGCTGTGCTCGGGGATCAGTTCTCGAAGACGACCATCAATCACATTGAGAAAGGTTCGGCTTTGCTTTGGGTGCATTTCGTTTTCGTGGTTGTTGTTGTTGTTTTGGTGCATTTTAGTATTTCTGCCATTGAAGGGAAATTGAGAATCACTAGGATTAGGGATGGGAATGGCAATTTGAGTGACCCAACTGTGAATTCCACGGCTATTTTTACTATAATGGTGCAGGGGATACCGAAAACTATAGGTAATGATAGGACTGTGTTGCATGAGTATTTCCAGCATAGGTATCCTGGCAAGGTTTATAGGGTTATAATGCCGATGGATTTGTGTGCGTTGGATGAATTAGCCTCGGAATTAGTGAGGGTTAGGCATGAAATTTCTTGGTTGGTTGCACGAATTGACTCCCGGCTCTTGCCGTTTGAAAGTGAAGAGGATGGATATGTAAGGACTTCTTCAGAGGGGGTATGGGGCTGTGCTTGTAATTTATGGCATAAGGTGGAAGATTTCTGGTATAATACTCTGGCTAGGTTGGGATACACTGACGAAAGGAAGTTAGGAGAGTTGCAGGGATTGAGAGCTGAATTGGAGACTGAATTAGCTGCTTACAAAGAAGGTCGTGCACTGGGTGCTGGAGTTGCGTTTGTGGTGTTTAAGGATGTGTACACTGCTAATAAAGCTGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAGTTTTTTTCTCTTGTGGAATTGCGTTTACAGAGAAACCAATGGAAAGTTGAGCAGGCACCCTTGGCCACAGATATTTATTGGAATCATTTGGGGTCGTCAAAGCTCTCACTGAAATTGCGAAGAGTGTTGGTGAACACGTGCTTGCTGTTGATTCTTCTGTTCTTTAGCTCTCCTCTGGCAATTATCAGTGCTCTAAAAAATGCCTGGCGGATTATAAATGCGGAAGCTATGGATAACGCCCAGTTGTGGTTTGCTTGGGTGCAGAGCTCAAGCTGGCTCGGGAGTCTTATCTTTCAGTTTCTACCCAATGTGTTTATTTTTGTCAGCATGTATATAGTTATTCCATCAGCACTTTCATATCTCTCCAAGTTCGAGCGACACCTTACAGTGTCAGGGGAGCAAAGAGCTGCTCTTCTTAAGATGGTTTGTTTCTTCCTGGTGAATCTTATACTTCTTAAGGGACTGGTTGAATCATCTTTAGAGAGTGCCATATTAAAAATGGGAAAGTTGCCTCGTTTTTGTCAAGATCGATCTCTTTCCTCTCTTGCATTCTTGATAACAAGTACATTCTTGGGTATATCTTATGATCTATTGGCTCCAATCCCTTGGATAAAAAGGAAGATTCAGAAGTTTCGAAAGAATGACATGCTACAGCTGGTCCCGGAACAAAGTGAAGAATATGCATTGGAAACTCAGGAAACCGATAGTCTAGAAAGGCCCTTGATTGTGGACACCACTTACGATTCTCCCAGATCGTATGGAATTGATCTGCAGGGAAATGATCTTTCTGATTACCCAATCAACAGAACATCAACAGCCCCAAAACAGACATTCGATTTTGCCCAATATTATGCCTTTAATTTGACGATATTCGCCCTGACCTTTATATATTCTTCCTTCTCTCCGCTTGTGGTTCCCGTCGGTGCTATTTATTTTGGGTATAGGTACGTTGTCGATAAGTACAACTTCCTCTTTGTCTATAGGGTCCGTGGCTTTCCTGCTGGGAATGATGGGAAGTTGATGGATACAGTATTGAGCATCATGCGTTTTTGTGTCGATCTGTTCCTTCTTGCAATGCTGCTGTTCTTTTCGGTCCATGGAGACTCAACGAAGCTGCAAGCTATATTTACACTTGGGGTACTAGTAATGTATAAATTGCTGCCTTCCCAGAATGATAGTCTTCATCCAGCTCTTTTGGAAGGCATACAGACTGTAGACACTGTTGTTGATGGGCCTATTGACTATGAGGTGTATTCACAGCCCAGATTTGATTGGGATACATATTATTCATGTTTCACCATTGGTTGA

Protein Analysis

794

Amino Acids

89.83

Weight (kDa)

6.75

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 51 - 158 1.7e-11 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 188 - 387 2.1e-11 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 398 - 568 1.9e-10 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 633 - 721 3.1e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35870
fragaria_vesca FvH4_1g11170
malus_domestica MD02G1124200.v1.1 MD15G1238200.v1.1
prunus_persica Prupe.7G174200_v2.0.a1
pyrus_communis pycom02g09710 pycom05g15420 pycom15g21190
rosa_chinensis RchiOBHm_Chr2g0098461
rosa_laevigata RLG00000016800
rosa_multiflora Rmu_sc0010938.1_g000006
rosa_roxburghii Rroxscaffold_2G00143940
rosa_rugosa Rorug02G0073400
rosa_samantha Rh2AG120700 Rh2DG125700
rosa_wichuraiana Rw2G009460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 665, 1289
AccB1I GGYRCC 1 cut(s) 1129
AccI GTMKAC 1 cut(s) 2289
AclWI GGATC 1 cut(s) 375
AcoI YGGCCR 1 cut(s) 1137
AcsI RAATTY 2 cut(s) 547, 729
AcuI CTGAAG 1 cut(s) 792
AcvI CACGTG 1 cut(s) 1212
AcyI GRCGYC 2 cut(s) 42, 249
AdeI CACNNNGTG 1 cut(s) 980
AfaI GTAC 7 cut(s) 65, 80, 1016, 1639, 2021, 2036, 2214
AfiI CCNNNNNNNGG 4 cut(s) 32, 66, 486, 1320
AflII CTTAAG 2 cut(s) 1490, 1529
AflIII ACRYGT 1 cut(s) 1209
AgsI TTSAA 3 cut(s) 485, 776, 1544
AhlI ACTAGT 1 cut(s) 2215
AjnI CCWGG 4 cut(s) 59, 643, 1277, 1509
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
Alw21I GWGCWC 4 cut(s) 363, 976, 1267, 1343
Alw26I GTCTC 2 cut(s) 935, 2169
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 1 cut(s) 375
Ama87I CYCGRG 2 cut(s) 362, 1352
AoxI GGCC 5 cut(s) 57, 169, 1137, 1790, 2306
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 6 cut(s) 147, 953, 1481, 2152, 2188, 2233
ApoI RAATTY 2 cut(s) 547, 729
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 6 cut(s) 170, 1732, 1791, 2059, 2167, 2306
AsuC2I CCSGG 5 cut(s) 33, 168, 349, 760, 1736
AsuHPI GGTGA 3 cut(s) 1216, 1525, 2362
AsuII TTCGAA 1 cut(s) 1708
AvaI CYCGRG 2 cut(s) 362, 1352
AvaII GGWCC 3 cut(s) 1732, 2059, 2167
BaeGI GKGCMC 1 cut(s) 976
BalI TGGCCA 1 cut(s) 1139
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 1 cut(s) 1343
BbrPI CACGTG 1 cut(s) 1212
BbsI GAAGAC 3 cut(s) 112, 386, 2246
Bbv12I GWGCWC 4 cut(s) 363, 976, 1267, 1343
BbvI GCAGC 6 cut(s) 159, 940, 1468, 2139, 2175, 2220
BceAI ACGGC 3 cut(s) 10, 570, 754
BciT130I CCWGG 4 cut(s) 61, 645, 1279, 1511
BciVI GTATCC 5 cut(s) 576, 651, 881, 2092, 2344
BcnI CCSGG 5 cut(s) 33, 168, 349, 760, 1736
BcoDI GTCTC 2 cut(s) 935, 2169
BcuI ACTAGT 1 cut(s) 2215
BfaI CTAG 4 cut(s) 504, 879, 1784, 2216
BfmI CTRYAG 5 cut(s) 594, 1724, 1844, 2053, 2286
BfoI RGCGCY 1 cut(s) 246
BfrI CTTAAG 2 cut(s) 1490, 1529
BfuI GTATCC 5 cut(s) 576, 651, 881, 2092, 2344
BglI GCCNNNNNGGC 1 cut(s) 240
BisI GCNGC 9 cut(s) 148, 160, 241, 252, 954, 1482, 2153, 2189, 2234
BlsI GCNGC 9 cut(s) 149, 161, 242, 253, 955, 1483, 2154, 2190, 2235
Bme1390I CCNGG 9 cut(s) 33, 61, 168, 349, 645, 760, 1279, 1511, 1736
Bme18I GGWCC 3 cut(s) 1732, 2059, 2167
BmeT110I CYCGRG 2 cut(s) 362, 1352
BmgT120I GGNCC 6 cut(s) 170, 1732, 1791, 2059, 2167, 2306
BmiI GGNNCC 6 cut(s) 106, 1131, 1671, 1734, 1989, 2060
BmrFI CCNGG 9 cut(s) 33, 61, 168, 349, 645, 760, 1279, 1511, 1736
BmrI ACTGGG 2 cut(s) 986, 1309
BmsI GCATC 2 cut(s) 315, 2121
BmuI ACTGGG 2 cut(s) 986, 1309
BpiI GAAGAC 3 cut(s) 112, 386, 2246
BpmI CTGGAG 1 cut(s) 1005
Bpu14I TTCGAA 1 cut(s) 1708
BpuEI CTTGAG 1 cut(s) 1327
BpuMI CCSGG 5 cut(s) 33, 168, 349, 760, 1736
Bsa29I ATCGAT 1 cut(s) 1603
BsaAI YACGTR 1 cut(s) 1212
BsaHI GRCGYC 2 cut(s) 42, 249
BsaJI CCNNGG 8 cut(s) 31, 300, 552, 702, 1134, 1679, 2062, 2170
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 66, 486, 1320
Bse1I ACTGG 4 cut(s) 76, 981, 1315, 1542
Bse3DI GCAATG 1 cut(s) 2154
BseBI CCWGG 4 cut(s) 61, 645, 1279, 1511
BseCI ATCGAT 1 cut(s) 1603
BseDI CCNNGG 8 cut(s) 31, 300, 552, 702, 1134, 1679, 2062, 2170
BseGI GGATG 7 cut(s) 72, 173, 520, 697, 793, 1015, 2257
BseLI CCNNNNNNNGG 4 cut(s) 32, 66, 486, 1320
BseMI GCAATG 1 cut(s) 2154
BseMII CTCAG 1 cut(s) 1781
BseNI ACTGG 4 cut(s) 76, 981, 1315, 1542
BseRI GAGGAG 2 cut(s) 9, 1236
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 6 cut(s) 159, 940, 1468, 2139, 2175, 2220
BseYI CCCAGC 1 cut(s) 2075
BsgI GTGCAG 2 cut(s) 596, 1355
BshFI GGCC 5 cut(s) 59, 171, 1139, 1792, 2308
BshNI GGYRCC 1 cut(s) 1129
BshVI ATCGAT 1 cut(s) 1603
BsiHKAI GWGCWC 4 cut(s) 363, 976, 1267, 1343
BsiHKCI CYCGRG 2 cut(s) 362, 1352
BsiSI CCGG 5 cut(s) 33, 168, 348, 760, 1736
BslFI GGGAC 3 cut(s) 13, 1548, 1718
BslI CCNNNNNNNGG 4 cut(s) 32, 66, 486, 1320
BsmAI GTCTC 2 cut(s) 935, 2169
BsmFI GGGAC 3 cut(s) 13, 1548, 1718
BsmI GAATGC 1 cut(s) 1622
BsnI GGCC 5 cut(s) 59, 171, 1139, 1792, 2308
BsoBI CYCGRG 2 cut(s) 362, 1352
Bsp119I TTCGAA 1 cut(s) 1708
Bsp1286I GDGCHC 4 cut(s) 363, 976, 1267, 1343
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
Bsp19I CCATGG 2 cut(s) 300, 2170
BspANI GGCC 5 cut(s) 59, 171, 1139, 1792, 2308
BspCNI CTCAG 1 cut(s) 1780
BspDI ATCGAT 1 cut(s) 1603
BspLI GGNNCC 6 cut(s) 106, 1131, 1671, 1734, 1989, 2060
BspMAI CTGCAG 1 cut(s) 1848
BspPI GGATC 1 cut(s) 375
BspQI GCTCTTC 1 cut(s) 1491
BspT104I TTCGAA 1 cut(s) 1708
BspT107I GGYRCC 1 cut(s) 1129
BspTI CTTAAG 2 cut(s) 1490, 1529
BsrDI GCAATG 1 cut(s) 2154
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 4 cut(s) 76, 981, 1315, 1542
BssECI CCNNGG 8 cut(s) 31, 300, 552, 702, 1134, 1679, 2062, 2170
BssMI GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
BssNI GRCGYC 2 cut(s) 42, 249
BssT1I CCWWGG 4 cut(s) 300, 1134, 1679, 2170
Bst2UI CCWGG 4 cut(s) 61, 645, 1279, 1511
Bst4CI ACNGT 6 cut(s) 544, 613, 1462, 2104, 2287, 2296
Bst6I CTCTTC 4 cut(s) 777, 1052, 1189, 1491
BstACI GRCGYC 2 cut(s) 42, 249
BstAFI CTTAAG 2 cut(s) 1490, 1529
BstAPI GCANNNNNTGC 1 cut(s) 980
BstAUI TGTACA 1 cut(s) 1014
BstBAI YACGTR 1 cut(s) 1212
BstBI TTCGAA 1 cut(s) 1708
BstC8I GCNNGC 7 cut(s) 164, 210, 232, 1128, 1217, 1349, 2193
BstDEI CTNAG 1 cut(s) 1767
BstDSI CCRYGG 4 cut(s) 300, 552, 2062, 2170
BstF5I GGATG 7 cut(s) 72, 173, 520, 697, 793, 1015, 2257
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 8 cut(s) 370, 1603, 1607, 1663, 1829, 1843, 1858, 2134
BstMAI GTCTC 2 cut(s) 935, 2169
BstMBI GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
BstNI CCWGG 4 cut(s) 61, 645, 1279, 1511
BstNSI RCATGY 3 cut(s) 166, 1405, 1723
BstSCI CCNGG 9 cut(s) 31, 59, 166, 347, 643, 758, 1277, 1509, 1734
BstSFI CTRYAG 5 cut(s) 594, 1724, 1844, 2053, 2286
BstSLI GKGCMC 1 cut(s) 976
BstV1I GCAGC 6 cut(s) 159, 940, 1468, 2139, 2175, 2220
BstV2I GAAGAC 3 cut(s) 112, 386, 2246
BstXI CCANNNNNNTGG 1 cut(s) 1680
Bsu15I ATCGAT 1 cut(s) 1603
BsuI GTATCC 5 cut(s) 576, 651, 881, 2092, 2344
BsuRI GGCC 5 cut(s) 59, 171, 1139, 1792, 2308
BsuTUI ATCGAT 1 cut(s) 1603
BtgI CCRYGG 4 cut(s) 300, 552, 2062, 2170
BtsCI GGATG 7 cut(s) 72, 173, 520, 697, 793, 1015, 2257
BtsI GCAGTG 2 cut(s) 235, 1017
BtsIMutI CAGTG 8 cut(s) 235, 891, 974, 1017, 1181, 1267, 1467, 2292
Cac8I GCNNGC 7 cut(s) 164, 210, 232, 1128, 1217, 1349, 2193
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 6 cut(s) 170, 1732, 1791, 2059, 2167, 2306
ClaI ATCGAT 1 cut(s) 1603
CseI GACGC 3 cut(s) 50, 106, 257
Csp6I GTAC 7 cut(s) 64, 79, 1015, 1638, 2020, 2035, 2213
CviQI GTAC 7 cut(s) 64, 79, 1015, 1638, 2020, 2035, 2213
DdeI CTNAG 1 cut(s) 1767
DpnI GATC 8 cut(s) 369, 1602, 1606, 1662, 1828, 1842, 1857, 2133
DpnII GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
DraIII CACNNNGTG 1 cut(s) 980
EaeI YGGCCR 1 cut(s) 1137
Eam1104I CTCTTC 4 cut(s) 777, 1052, 1189, 1491
EarI CTCTTC 4 cut(s) 777, 1052, 1189, 1491
EciI GGCGGA 5 cut(s) 12, 85, 166, 1078, 1297
Ecl136II GAGCTC 1 cut(s) 1341
Eco130I CCWWGG 4 cut(s) 300, 1134, 1679, 2170
Eco147I AGGCCT 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 1343
Eco47I GGWCC 3 cut(s) 1732, 2059, 2167
Eco53kI GAGCTC 1 cut(s) 1341
Eco57I CTGAAG 1 cut(s) 792
Eco72I CACGTG 1 cut(s) 1212
Eco88I CYCGRG 2 cut(s) 362, 1352
EcoICRI GAGCTC 1 cut(s) 1341
EcoO109I RGGNCCY 1 cut(s) 1791
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 4 cut(s) 59, 643, 1277, 1509
EcoT14I CCWWGG 4 cut(s) 300, 1134, 1679, 2170
EcoT22I ATGCAT 1 cut(s) 1759
EcoT38I GRGCYC 1 cut(s) 1343
ErhI CCWWGG 4 cut(s) 300, 1134, 1679, 2170
FalI AAGNNNNNCTT 4 cut(s) 790, 822, 2024, 2056
FaqI GGGAC 3 cut(s) 13, 1548, 1718
FblI GTMKAC 1 cut(s) 2289
Fnu4HI GCNGC 9 cut(s) 148, 160, 241, 252, 954, 1482, 2153, 2189, 2234
FokI GGATG 7 cut(s) 59, 160, 527, 704, 800, 1022, 2244
FriOI GRGCYC 1 cut(s) 1343
Fsp4HI GCNGC 9 cut(s) 148, 160, 241, 252, 954, 1482, 2153, 2189, 2234
FspBI CTAG 4 cut(s) 504, 879, 1784, 2216
GlaI GCGC 1 cut(s) 244
GluI GCNGC 9 cut(s) 148, 160, 241, 252, 954, 1482, 2153, 2189, 2234
GsaI CCCAGC 1 cut(s) 2079
GsuI CTGGAG 1 cut(s) 1005
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 5 cut(s) 59, 171, 1139, 1792, 2308
HapII CCGG 5 cut(s) 33, 168, 348, 760, 1736
HgaI GACGC 3 cut(s) 50, 106, 257
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 2 cut(s) 42, 249
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HpaII CCGG 5 cut(s) 33, 168, 348, 760, 1736
HphI GGTGA 3 cut(s) 1216, 1525, 2362
Hpy166II GTNNAC 7 cut(s) 974, 1015, 1017, 1100, 1207, 1804, 2290
Hpy188I TCNGA 4 cut(s) 706, 811, 1701, 1864
Hpy188III TCNNGA 9 cut(s) 221, 265, 376, 1037, 1354, 1598, 1627, 1769, 1784
Hpy8I GTNNAC 7 cut(s) 974, 1015, 1017, 1100, 1207, 1804, 2290
Hpy99I CGWCG 4 cut(s) 41, 44, 188, 1997
HpyAV CCTTC 8 cut(s) 92, 118, 479, 959, 1981, 2150, 2247, 2267
HpyCH4III ACNGT 6 cut(s) 544, 613, 1462, 2104, 2287, 2296
HpyCH4IV ACGT 2 cut(s) 1211, 2022
HpyF3I CTNAG 1 cut(s) 1767
HpySE526I ACGT 2 cut(s) 1211, 2022
Hsp92I GRCGYC 2 cut(s) 42, 249
HspAI GCGC 1 cut(s) 243
Kzo9I GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
LguI GCTCTTC 1 cut(s) 1491
LmnI GCTCC 5 cut(s) 110, 147, 198, 1471, 1675
Lsp1109I GCAGC 6 cut(s) 159, 940, 1468, 2139, 2175, 2220
LweI GCATC 2 cut(s) 315, 2121
MaeI CTAG 4 cut(s) 504, 879, 1784, 2216
MaeII ACGT 2 cut(s) 1211, 2022
MaeIII GTNAC 1 cut(s) 533
MalI GATC 8 cut(s) 369, 1602, 1606, 1662, 1828, 1842, 1857, 2133
MboI GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
MhlI GDGCHC 4 cut(s) 363, 976, 1267, 1343
MlsI TGGCCA 1 cut(s) 1139
MluNI TGGCCA 1 cut(s) 1139
MlyI GAGTC 3 cut(s) 749, 1366, 2171
MmeI TCCRAC 1 cut(s) 669
Mox20I TGGCCA 1 cut(s) 1139
Mph1103I ATGCAT 1 cut(s) 1759
MscI TGGCCA 1 cut(s) 1139
MseI TTAA 5 cut(s) 1005, 1491, 1530, 1568, 1932
Msp20I TGGCCA 1 cut(s) 1139
MspA1I CMGCKG 2 cut(s) 334, 1729
MspCI CTTAAG 2 cut(s) 1490, 1529
MspI CCGG 5 cut(s) 33, 168, 348, 760, 1736
MspR9I CCNGG 9 cut(s) 33, 61, 168, 349, 645, 760, 1279, 1511, 1736
Mva1269I GAATGC 1 cut(s) 1622
MvaI CCWGG 4 cut(s) 61, 645, 1279, 1511
NciI CCSGG 5 cut(s) 33, 168, 349, 760, 1736
NcoI CCATGG 2 cut(s) 300, 2170
NdeII GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
NlaIV GGNNCC 6 cut(s) 106, 1131, 1671, 1734, 1989, 2060
NmuCI GTSAC 1 cut(s) 533
NsiI ATGCAT 1 cut(s) 1759
NspI RCATGY 3 cut(s) 166, 1405, 1723
NspV TTCGAA 1 cut(s) 1708
PaeI GCATGC 1 cut(s) 166
PceI AGGCCT 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 1491
PctI GAATGC 1 cut(s) 1622
PfeI GAWTC 8 cut(s) 337, 498, 1156, 1225, 1516, 1544, 1696, 1817
PfoI TCCNGGA 1 cut(s) 1734
PkrI GCNGC 9 cut(s) 149, 161, 242, 253, 955, 1483, 2154, 2190, 2235
PleI GAGTC 3 cut(s) 749, 1365, 2171
PmaCI CACGTG 1 cut(s) 1212
PmlI CACGTG 1 cut(s) 1212
PpsI GAGTC 3 cut(s) 749, 1365, 2171
Ppu21I YACGTR 1 cut(s) 1212
PsiI TTATAA 2 cut(s) 665, 1289
Psp124BI GAGCTC 1 cut(s) 1343
Psp6I CCWGG 4 cut(s) 59, 643, 1277, 1509
PspCI CACGTG 1 cut(s) 1212
PspFI CCCAGC 1 cut(s) 2075
PspGI CCWGG 4 cut(s) 59, 643, 1277, 1509
PspN4I GGNNCC 6 cut(s) 106, 1131, 1671, 1734, 1989, 2060
PspPI GGNCC 6 cut(s) 170, 1732, 1791, 2059, 2167, 2306
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PstI CTGCAG 1 cut(s) 1848
PvuII CAGCTG 1 cut(s) 1729
RsaI GTAC 7 cut(s) 65, 80, 1016, 1639, 2021, 2036, 2214
RsaNI GTAC 7 cut(s) 64, 79, 1015, 1638, 2020, 2035, 2213
SacI GAGCTC 1 cut(s) 1343
SapI GCTCTTC 1 cut(s) 1491
SaqAI TTAA 5 cut(s) 1005, 1491, 1530, 1568, 1932
SatI GCNGC 9 cut(s) 148, 160, 241, 252, 954, 1482, 2153, 2189, 2234
Sau3AI GATC 8 cut(s) 367, 1600, 1604, 1660, 1826, 1840, 1855, 2131
Sau96I GGNCC 6 cut(s) 170, 1732, 1791, 2059, 2167, 2306
SchI GAGTC 3 cut(s) 749, 1366, 2171
ScrFI CCNGG 9 cut(s) 33, 61, 168, 349, 645, 760, 1279, 1511, 1736
SduI GDGCHC 4 cut(s) 363, 976, 1267, 1343
SfaNI GCATC 2 cut(s) 315, 2121
SfcI CTRYAG 5 cut(s) 594, 1724, 1844, 2053, 2286
SfuI TTCGAA 1 cut(s) 1708
SinI GGWCC 3 cut(s) 1732, 2059, 2167
SmlI CTYRAG 3 cut(s) 1342, 1490, 1529
SmoI CTYRAG 3 cut(s) 1342, 1490, 1529
SpeI ACTAGT 1 cut(s) 2215
SphI GCATGC 1 cut(s) 166
SseBI AGGCCT 1 cut(s) 59
SspI AATATT 1 cut(s) 1922
SspMI CTAG 4 cut(s) 504, 879, 1784, 2216
SstI GAGCTC 1 cut(s) 1343
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 9 cut(s) 31, 59, 166, 347, 643, 758, 1277, 1509, 1734
StyI CCWWGG 4 cut(s) 300, 1134, 1679, 2170
TaaI ACNGT 6 cut(s) 544, 613, 1462, 2104, 2287, 2296
TaiI ACGT 2 cut(s) 1214, 2025
TaqI TCGA 8 cut(s) 377, 1045, 1446, 1603, 1708, 1908, 2028, 2130
TaqII GACCGA 1 cut(s) 2155
TatI WGTACW 3 cut(s) 1014, 1637, 2034
TauI GCSGC 3 cut(s) 162, 243, 254
TfiI GAWTC 8 cut(s) 337, 498, 1156, 1225, 1516, 1544, 1696, 1817
Tru1I TTAA 5 cut(s) 1005, 1491, 1530, 1568, 1932
Tru9I TTAA 5 cut(s) 1005, 1491, 1530, 1568, 1932
TscAI CASTG 8 cut(s) 242, 898, 981, 1024, 1188, 1267, 1467, 2299
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 6 cut(s) 147, 953, 1481, 2152, 2188, 2233
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 7 cut(s) 17, 118, 708, 741, 1419, 2246, 2352
TspGWI ACGGA 1 cut(s) 2051
TspRI CASTG 8 cut(s) 242, 898, 981, 1024, 1188, 1267, 1467, 2299
Vha464I CTTAAG 2 cut(s) 1490, 1529
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 3 cut(s) 1732, 2059, 2167
XapI RAATTY 2 cut(s) 547, 729
XbaI TCTAGA 1 cut(s) 1783
XceI RCATGY 3 cut(s) 166, 1405, 1723
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 1570
XmiI GTMKAC 1 cut(s) 2289
XspI CTAG 4 cut(s) 504, 879, 1784, 2216
Zsp2I ATGCAT 1 cut(s) 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.