pycom15g21910

UPF0183 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014288
Physical Location & Seq
Reverse (-)
15929743 .. 15933391
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGTTGCAGAGACCTCGACGCCGCTGCGAAGGCACCGCCATGGGCGCCATCGTCCTCGATCTACGACCCGGCCTCGGAATCGGACCCTTCACCATCGGAATGCCGATATGCGAAGCATTTGCTCAGATAGAGCAGGAGCCTAACATTTACGACGTTGTCCATGTGAAGTATTATGATGAGGAGCCCCTTAAGTTGGATATCGTTATCAGCTTTCCAGATCATGGTTTTCATCTTCGCTTTGATCCTTGGTCACAGAGGCTACGCCTTGTTGAAATATTTGATATAAAACGACTTCAAATGCGCTATGCCAATTCTTTGATTGGGGGACCATCCACTCTAGCAACTTTTGTAGCTGTATATGCACTATTTGGGCCAACTTTCCCTGGAATTTATGACAAGGATAGAGGTGTCTACACTCTGTTTTACCCAGGGCTTTCCTTTGCTTTTCCAATTCCGAGCCAGTATACAGATTGCTGCCTAGATAGAGAAGCGGAATTACCATTAGAGTTTCCAGATGGAACCACACCAGTGACATGCCGCGTCTCCATATACGATAGTTCTACAGATAAAAAAGTTGGTGTGGGGTCCTTAATGGATAAGGCTTCCGCTCCTCCACTACCTGTTGGCAGCCTCTATATGGAAGAGGTGCATGTTAAGCTAGGGGAAGAATTATACTTTACTGTTGGTGGTCAGAATATTCCTTTTGGTGCATCACCTCAGGATGTTTGGACTGAATTGGGTCGTCCTTGTGGGATACATCAAAAGCAGGTAGACCAAATGGTTATTCATTCTGGCTTGGACACGCGCCCACGGACAACTCTTTGTGGCGATTATTTCTACAATTACTTTACCCGTGGTTTGGACATTTTATTTGATGGGCAGACTCATAAAATCAAGAAGTTTGTTTTGCATACAAACTATCCTGGCCATGCAGATTTCAACTCGTACATAAAGTGCAATTTTGTCATATTTGCTTCTGATTTAGGGGGTTCTTATCAGGATGTCAATAACTGCAAACATAGGATTACAGCAAGCACAAAGTGGGAGCAAGTGAAGGAATTCCTTGGGGACTGTGGCCGAGCTGCTATCCAGACTCAAGGTTCTACAAGCAATCCGTTTGGATCTACTTTTGTATATGGTTATCAAAATGCTGCATTTGAGGTGATGAAGAATGGTTACATTGCCACTGTGACCCTTTTCCAGTCGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0183

Protein Analysis

404

Amino Acids

45.17

Weight (kDa)

5.82

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHAF1 PF03676 27 - 400 4.3e-139 Phagosome assembly factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51130
fragaria_vesca FvH4_1g12160 FvH4_1g12160 FvH4_1g12160
malus_domestica MD02G1111100.v1.1 MD15G1249000.v1.1
prunus_persica Prupe.7G165200_v2.0.a1
pyrus_communis pycom15g21910
rosa_chinensis RchiOBHm_Chr2g0099911
rosa_laevigata RLG00000016921
rosa_roxburghii Rroxscaffold_2G00142740
rosa_rugosa Rorug02G0083900
rosa_samantha Rh2AG132900 Rh2BG136300 Rh2CG138000 Rh2DG138400
rosa_wichuraiana Rw2G010340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 757
AccB1I GGYRCC 2 cut(s) 32, 44
AccB7I CCANNNNNTGG 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 608
AccI GTMKAC 3 cut(s) 411, 464, 771
AccII CGCG 2 cut(s) 540, 805
AciI CCGC 5 cut(s) 22, 36, 491, 538, 606
AclWI GGATC 2 cut(s) 236, 1129
AcoI YGGCCR 2 cut(s) 925, 1075
AcsI RAATTY 2 cut(s) 387, 1058
AcyI GRCGYC 2 cut(s) 19, 45
AdeI CACNNNGTG 1 cut(s) 1041
AfaI GTAC 1 cut(s) 947
AfiI CCNNNNNNNGG 5 cut(s) 74, 193, 221, 637, 859
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 3 cut(s) 272, 296, 940
AjnI CCWGG 3 cut(s) 382, 427, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 768, 800
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 4 cut(s) 210, 353, 658, 1082
AluI AGCT 4 cut(s) 210, 353, 658, 1082
Alw26I GTCTC 2 cut(s) 4, 547
AlwI GGATC 2 cut(s) 236, 1129
AoxI GGCC 4 cut(s) 70, 371, 925, 1075
ApeKI GCWGC 5 cut(s) 24, 474, 627, 1082, 1151
ApoI RAATTY 2 cut(s) 387, 1058
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
Asp700I GAANNNNTTC 1 cut(s) 1058
AspLEI GCGC 3 cut(s) 47, 303, 807
AspS9I GGNCC 4 cut(s) 83, 326, 371, 585
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 3 cut(s) 82, 705, 1174
AvaII GGWCC 3 cut(s) 83, 326, 585
AxyI CCTNAGG 1 cut(s) 717
BalI TGGCCA 1 cut(s) 927
BanI GGYRCC 2 cut(s) 32, 44
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 4 cut(s) 480, 512, 1160, 1192
BbvI GCAGC 5 cut(s) 11, 461, 639, 1069, 1138
BccI CCATC 5 cut(s) 56, 101, 337, 509, 869
BcgI CGANNNNNNTGC 5 cut(s) 6, 30, 40, 101, 135
BciT130I CCWGG 3 cut(s) 384, 429, 924
BciVI GTATCC 1 cut(s) 747
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 4, 547
BfaI CTAG 3 cut(s) 338, 479, 659
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 757
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 7 cut(s) 22, 25, 475, 538, 628, 1083, 1152
BlsI GCNGC 7 cut(s) 23, 26, 476, 539, 629, 1084, 1153
Bme1390I CCNGG 4 cut(s) 69, 384, 429, 924
Bme18I GGWCC 3 cut(s) 83, 326, 585
BmgT120I GGNCC 4 cut(s) 83, 326, 371, 585
BmiI GGNNCC 8 cut(s) 34, 46, 85, 138, 183, 327, 520, 586
BmrFI CCNGG 4 cut(s) 69, 384, 429, 924
BmsI GCATC 1 cut(s) 719
BpuEI CTTGAG 1 cut(s) 1080
BpuMI CCSGG 1 cut(s) 69
BsaHI GRCGYC 2 cut(s) 19, 45
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 9 cut(s) 39, 73, 245, 382, 427, 428, 809, 853, 1063
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 193, 221, 637, 859
Bse1I ACTGG 3 cut(s) 460, 527, 1201
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 1179
BseBI CCWGG 3 cut(s) 384, 429, 924
BseDI CCNNGG 9 cut(s) 39, 73, 245, 382, 427, 428, 809, 853, 1063
BseGI GGATG 3 cut(s) 329, 727, 1006
BseLI CCNNNNNNNGG 5 cut(s) 74, 193, 221, 637, 859
BseMI GCAATG 1 cut(s) 1179
BseMII CTCAG 2 cut(s) 137, 731
BseNI ACTGG 3 cut(s) 460, 527, 1201
BseRI GAGGAG 2 cut(s) 194, 600
BseXI GCAGC 5 cut(s) 11, 461, 639, 1069, 1138
Bsh1236I CGCG 2 cut(s) 540, 805
BshFI GGCC 4 cut(s) 72, 373, 927, 1077
BshNI GGYRCC 2 cut(s) 32, 44
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 2 cut(s) 339, 1082
BslI CCNNNNNNNGG 5 cut(s) 74, 193, 221, 637, 859
BsmAI GTCTC 2 cut(s) 4, 547
BsmBI CGTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 339, 1082
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 4 cut(s) 72, 373, 927, 1077
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 4 cut(s) 58, 217, 241, 1121
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 5 cut(s) 22, 36, 491, 538, 606
BspANI GGCC 4 cut(s) 72, 373, 927, 1077
BspCNI CTCAG 2 cut(s) 136, 730
BspFNI CGCG 2 cut(s) 540, 805
BspLI GGNNCC 8 cut(s) 34, 46, 85, 138, 183, 327, 520, 586
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 2 cut(s) 236, 1129
BspT107I GGYRCC 2 cut(s) 32, 44
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 1179
BsrI ACTGG 3 cut(s) 460, 527, 1201
BssECI CCNNGG 9 cut(s) 39, 73, 245, 382, 427, 428, 809, 853, 1063
BssMI GATC 4 cut(s) 58, 217, 241, 1121
BssNAI GTATAC 1 cut(s) 465
BssNI GRCGYC 2 cut(s) 19, 45
BssT1I CCWWGG 3 cut(s) 39, 245, 1063
Bst1107I GTATAC 1 cut(s) 465
Bst2UI CCWGG 3 cut(s) 384, 429, 924
Bst4CI ACNGT 3 cut(s) 682, 1073, 1189
Bst6I CTCTTC 1 cut(s) 636
BstACI GRCGYC 2 cut(s) 19, 45
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 1033
BstDEI CTNAG 2 cut(s) 123, 717
BstDSI CCRYGG 3 cut(s) 39, 809, 853
BstF5I GGATG 3 cut(s) 329, 727, 1006
BstFNI CGCG 2 cut(s) 540, 805
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 3 cut(s) 47, 303, 807
BstKTI GATC 4 cut(s) 61, 220, 244, 1124
BstMAI GTCTC 2 cut(s) 4, 547
BstMBI GATC 4 cut(s) 58, 217, 241, 1121
BstMWI GCNNNNNNNGC 4 cut(s) 30, 44, 359, 655
BstNI CCWGG 3 cut(s) 384, 429, 924
BstNSI RCATGY 2 cut(s) 537, 653
BstSCI CCNGG 4 cut(s) 67, 382, 427, 922
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 2 cut(s) 540, 805
BstV1I GCAGC 5 cut(s) 11, 461, 639, 1069, 1138
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BstZ17I GTATAC 1 cut(s) 465
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 4 cut(s) 72, 373, 927, 1077
BtgI CCRYGG 3 cut(s) 39, 809, 853
BtsCI GGATG 3 cut(s) 329, 727, 1006
BtsIMutI CAGTG 2 cut(s) 534, 1185
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 1 cut(s) 1033
CfoI GCGC 3 cut(s) 47, 303, 807
Cfr13I GGNCC 4 cut(s) 83, 326, 371, 585
CseI GACGC 2 cut(s) 27, 529
Csp6I GTAC 1 cut(s) 946
CviAII CATG 6 cut(s) 40, 161, 221, 534, 650, 929
CviQI GTAC 1 cut(s) 946
DdeI CTNAG 2 cut(s) 123, 717
DinI GGCGCC 1 cut(s) 46
DpnI GATC 4 cut(s) 60, 219, 243, 1123
DpnII GATC 4 cut(s) 58, 217, 241, 1121
DraIII CACNNNGTG 1 cut(s) 1041
EaeI YGGCCR 2 cut(s) 925, 1075
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco130I CCWWGG 3 cut(s) 39, 245, 1063
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 199
Eco47I GGWCC 3 cut(s) 83, 326, 585
Eco81I CCTNAGG 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 585
EcoRI GAATTC 1 cut(s) 1058
EcoRII CCWGG 3 cut(s) 382, 427, 922
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 3 cut(s) 39, 245, 1063
EcoT38I GRGCYC 1 cut(s) 186
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 3 cut(s) 39, 245, 1063
Esp3I CGTCTC 1 cut(s) 547
FaeI CATG 6 cut(s) 43, 164, 224, 537, 653, 932
FaqI GGGAC 2 cut(s) 339, 1082
FatI CATG 6 cut(s) 39, 160, 220, 533, 649, 928
FblI GTMKAC 3 cut(s) 411, 464, 771
Fnu4HI GCNGC 7 cut(s) 22, 25, 475, 538, 628, 1083, 1152
FokI GGATG 3 cut(s) 316, 734, 1013
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 7 cut(s) 22, 25, 475, 538, 628, 1083, 1152
FspBI CTAG 3 cut(s) 338, 479, 659
GlaI GCGC 3 cut(s) 46, 302, 806
GluI GCNGC 7 cut(s) 22, 25, 475, 538, 628, 1083, 1152
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 4 cut(s) 72, 373, 927, 1077
HapII CCGG 1 cut(s) 69
HgaI GACGC 2 cut(s) 27, 529
HhaI GCGC 3 cut(s) 47, 303, 807
Hin1I GRCGYC 2 cut(s) 19, 45
Hin1II CATG 6 cut(s) 43, 164, 224, 537, 653, 932
Hin6I GCGC 3 cut(s) 45, 301, 805
HinP1I GCGC 3 cut(s) 45, 301, 805
HinfI GANTC 3 cut(s) 78, 883, 1093
HpaII CCGG 1 cut(s) 69
HphI GGTGA 3 cut(s) 82, 705, 1174
Hpy166II GTNNAC 3 cut(s) 412, 465, 772
Hpy188I TCNGA 7 cut(s) 77, 83, 98, 126, 456, 693, 979
Hpy188III TCNNGA 7 cut(s) 215, 512, 719, 895, 998, 1090, 1209
Hpy8I GTNNAC 3 cut(s) 412, 465, 772
Hpy99I CGWCG 3 cut(s) 21, 155, 1210
HpyAV CCTTC 3 cut(s) 23, 97, 1048
HpyCH4III ACNGT 3 cut(s) 682, 1073, 1189
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 9 cut(s) 7, 362, 649, 710, 910, 932, 957, 1014, 1154
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 44, 359, 655
HpyF3I CTNAG 2 cut(s) 123, 717
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 2 cut(s) 19, 45
Hsp92II CATG 6 cut(s) 43, 164, 224, 537, 653, 932
HspAI GCGC 3 cut(s) 45, 301, 805
KasI GGCGCC 1 cut(s) 44
Kzo9I GATC 4 cut(s) 58, 217, 241, 1121
LmnI GCTCC 4 cut(s) 136, 181, 613, 1045
Lsp1109I GCAGC 5 cut(s) 11, 461, 639, 1069, 1138
LweI GCATC 1 cut(s) 719
MaeI CTAG 3 cut(s) 338, 479, 659
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 4 cut(s) 249, 529, 1175, 1189
MalI GATC 4 cut(s) 60, 219, 243, 1123
MbiI CCGCTC 1 cut(s) 608
MboI GATC 4 cut(s) 58, 217, 241, 1121
MboII GAAGA 4 cut(s) 224, 653, 677, 1180
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 927
MluCI AATT 9 cut(s) 310, 387, 450, 494, 668, 734, 841, 958, 1058
MluNI TGGCCA 1 cut(s) 927
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 2 cut(s) 877, 1087
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 927
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 927
MseI TTAA 3 cut(s) 189, 590, 654
MslI CAYNNNNRTG 3 cut(s) 38, 98, 527
Msp20I TGGCCA 1 cut(s) 927
MspA1I CMGCKG 1 cut(s) 24
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 4 cut(s) 69, 384, 429, 924
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 3 cut(s) 384, 429, 924
MvnI CGCG 2 cut(s) 540, 805
MwoI GCNNNNNNNGC 4 cut(s) 30, 44, 359, 655
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 69
NcoI CCATGG 1 cut(s) 39
NdeII GATC 4 cut(s) 58, 217, 241, 1121
NlaIII CATG 6 cut(s) 43, 164, 224, 537, 653, 932
NlaIV GGNNCC 8 cut(s) 34, 46, 85, 138, 183, 327, 520, 586
NmeAIII GCCGAG 1 cut(s) 1103
NmuCI GTSAC 3 cut(s) 249, 529, 1189
NspI RCATGY 2 cut(s) 537, 653
OliI CACNNNNGTG 1 cut(s) 527
PasI CCCWGGG 1 cut(s) 428
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 1 cut(s) 78
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 7 cut(s) 23, 26, 476, 539, 629, 1084, 1153
PleI GAGTC 2 cut(s) 877, 1087
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 2 cut(s) 877, 1087
PpuMI RGGWCCY 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 585
Psp6I CCWGG 3 cut(s) 382, 427, 922
PspGI CCWGG 3 cut(s) 382, 427, 922
PspN4I GGNNCC 8 cut(s) 34, 46, 85, 138, 183, 327, 520, 586
PspPI GGNCC 4 cut(s) 83, 326, 371, 585
PspPPI RGGWCCY 1 cut(s) 585
PsuI RGATCY 1 cut(s) 1121
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 1 cut(s) 947
RsaNI GTAC 1 cut(s) 946
RseI CAYNNNNRTG 3 cut(s) 38, 98, 527
SaqAI TTAA 3 cut(s) 189, 590, 654
SatI GCNGC 7 cut(s) 22, 25, 475, 538, 628, 1083, 1152
Sau3AI GATC 4 cut(s) 58, 217, 241, 1121
Sau96I GGNCC 4 cut(s) 83, 326, 371, 585
SchI GAGTC 2 cut(s) 877, 1087
ScrFI CCNGG 4 cut(s) 69, 384, 429, 924
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 1 cut(s) 719
SfcI CTRYAG 1 cut(s) 561
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 3 cut(s) 83, 326, 585
SmiMI CAYNNNNRTG 3 cut(s) 38, 98, 527
SmlI CTYRAG 2 cut(s) 188, 1095
SmoI CTYRAG 2 cut(s) 188, 1095
Sse9I AATT 9 cut(s) 310, 387, 450, 494, 668, 734, 841, 958, 1058
SsiI CCGC 5 cut(s) 22, 36, 491, 538, 606
SspDI GGCGCC 1 cut(s) 44
SspI AATATT 2 cut(s) 276, 697
SspMI CTAG 3 cut(s) 338, 479, 659
StyD4I CCNGG 4 cut(s) 67, 382, 427, 922
StyI CCWWGG 3 cut(s) 39, 245, 1063
TaaI ACNGT 3 cut(s) 682, 1073, 1189
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 16, 57
TasI AATT 9 cut(s) 310, 387, 450, 494, 668, 734, 841, 958, 1058
TauI GCSGC 2 cut(s) 24, 540
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 3 cut(s) 189, 590, 654
Tru9I TTAA 3 cut(s) 189, 590, 654
TscAI CASTG 2 cut(s) 534, 1192
TseFI GTSAC 3 cut(s) 249, 529, 1189
TseI GCWGC 5 cut(s) 24, 474, 627, 1082, 1151
Tsp45I GTSAC 3 cut(s) 249, 529, 1189
TspDTI ATGAA 3 cut(s) 218, 776, 1181
TspGWI ACGGA 2 cut(s) 826, 1104
TspRI CASTG 2 cut(s) 534, 1192
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 221
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 3 cut(s) 83, 326, 585
XapI RAATTY 2 cut(s) 387, 1058
XceI RCATGY 2 cut(s) 537, 653
XmiI GTMKAC 3 cut(s) 411, 464, 771
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 338, 479, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.