Rh2DG138400

UPF0183 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014288
Physical Location & Seq
Reverse (-)
11547226 .. 11551479
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG138400.1

Sequence Viewer

Length: 1080 bp
ATGATGCAGAGACCCCGACGACGCTGCGAAGGCACCGCCATGGGCGCCATCGTCCTCGATCTTCGTCCCGGCTTCGGAATCGGACCTTTCTCTCTCGGAATGCCGATATGCGAAGCGTTTGCTCAGATAGAGCAGCAGCCTAACATCTATGATGTTGTTCATGTCAAGTATTATGATGAGGAGCCTCTTAAGTTGGACATTGTTATCAGCTTTCCTGATCATGGTTTCCATCTGCGATTTGACCCTTGGTCACAGAGACTCCGCCTTGTTGAAATATTTGACATAAAGAGGCTTCAGATGCGCTATGCCACGTCTTTGATTGGAGGCCCATCCACTCTGGCGACTTTCGTAGCTGTTTATGCCTTGTTTGGGCCAACATTTCCTGGAATTTATGATCAAGATAGAGGTGTCTACAATCTATTTTACCCAGGGCTTTCCTTTGCTTTTCCTATTCCAAGCCAGTATACAGATTGCGTTCATGATAGAGAAGCGGAACTACCTCTAGAGTTCCCAGATGGAACTACACCAGTTACTTGCCGTGTGTCCATATACGATAGTTCTTCCGATAAAAAAGTCGGTGTCGGATCCTTGATGGATAAGGCTTCTGCTCCTTTATTACCTAATGGCAGTGTCTATATGGAAGAGGTGCATGTCAAGCTAGCGGAAGAACTACTCTTCACTGTTGGGGGCCAGAATATTCCTTTTGGTGCATCACCTCAGGATGTTTGGACTGAATTGGGTCGTCCTTGTGGGATACATCAAAAGCAGGTAGACCAAATGGTCATTCATTCTGGCTCGGATCCTCGCCCACGGACAACATTGTGTGGTGATTACTTCTACAATTACTTTACCCGTGGTTTGGACATTTTATTTGATGGGCAGACTCATAAAGTCAAGAAGTTTGTTCTGCATACAAATTATCCTGGTCATGCCGATTTCAATTCGTATATAAAGTGCAACTTTGTCATCTTTGCTTCTGAGTTTGGGGGTTCTTTTCAGGAGGAAAATAATTGCAAGCACAAGATTACACCAAGTACAAAGTGGGAGCATGTGAAGGTAAAATTGCTCTTTACAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0183

Protein Analysis

359

Amino Acids

40.59

Weight (kDa)

6.06

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHAF1 PF03676 27 - 355 1.4e-120 Phagosome assembly factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51130
fragaria_vesca FvH4_1g12160 FvH4_1g12160 FvH4_1g12160
malus_domestica MD02G1111100.v1.1 MD15G1249000.v1.1
prunus_persica Prupe.7G165200_v2.0.a1
pyrus_communis pycom15g21910
rosa_chinensis RchiOBHm_Chr2g0099911
rosa_laevigata RLG00000016921
rosa_roxburghii Rroxscaffold_2G00142740
rosa_rugosa Rorug02G0083900
rosa_samantha Rh2AG132900 Rh2BG136300 Rh2CG138000 Rh2DG138400
rosa_wichuraiana Rw2G010340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 779
Acc36I ACCTGC 1 cut(s) 757
AccB1I GGYRCC 2 cut(s) 32, 44
AccI GTMKAC 3 cut(s) 411, 464, 771
AciI CCGC 4 cut(s) 36, 262, 491, 662
AclWI GGATC 4 cut(s) 579, 592, 794, 807
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 1 cut(s) 1036
AfiI CCNNNNNNNGG 4 cut(s) 74, 221, 369, 859
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 2 cut(s) 272, 940
AhdI GACNNNNNGTC 1 cut(s) 247
AjiI CACGTC 1 cut(s) 312
AjnI CCWGG 3 cut(s) 382, 427, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 768, 800
AluBI AGCT 3 cut(s) 210, 353, 658
AluI AGCT 3 cut(s) 210, 353, 658
Alw26I GTCTC 2 cut(s) 4, 250
AlwI GGATC 4 cut(s) 579, 592, 794, 807
AoxI GGCC 3 cut(s) 325, 371, 688
ApeKI GCWGC 3 cut(s) 24, 133, 136
ApoI RAATTY 1 cut(s) 387
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AspLEI GCGC 2 cut(s) 47, 303
AspS9I GGNCC 4 cut(s) 83, 326, 371, 688
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 2 cut(s) 705, 839
AsuNHI GCTAGC 1 cut(s) 658
AvaII GGWCC 1 cut(s) 83
AxyI CCTNAGG 1 cut(s) 717
BamHI GGATCC 2 cut(s) 584, 799
BanI GGYRCC 2 cut(s) 32, 44
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
BbvI GCAGC 3 cut(s) 11, 145, 148
BccI CCATC 6 cut(s) 56, 237, 337, 509, 586, 869
BceAI ACGGC 1 cut(s) 522
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 101, 135
BciT130I CCWGG 3 cut(s) 384, 429, 924
BciVI GTATCC 1 cut(s) 747
BclI TGATCA 2 cut(s) 217, 394
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 4, 250
BfaI CTAG 2 cut(s) 503, 659
BfoI RGCGCY 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 757
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 3 cut(s) 25, 134, 137
BlsI GCNGC 3 cut(s) 26, 135, 138
Bme1390I CCNGG 4 cut(s) 69, 384, 429, 924
Bme18I GGWCC 1 cut(s) 83
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 312
BmgT120I GGNCC 4 cut(s) 83, 326, 371, 688
BmiI GGNNCC 6 cut(s) 34, 46, 183, 586, 689, 801
BmrFI CCNGG 4 cut(s) 69, 384, 429, 924
BmsI GCATC 2 cut(s) 288, 719
BmtI GCTAGC 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 6 cut(s) 39, 245, 427, 428, 809, 853
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 221, 369, 859
Bse1I ACTGG 2 cut(s) 460, 527
Bse21I CCTNAGG 1 cut(s) 717
BseBI CCWGG 3 cut(s) 384, 429, 924
BseDI CCNNGG 6 cut(s) 39, 245, 427, 428, 809, 853
BseGI GGATG 2 cut(s) 329, 727
BseLI CCNNNNNNNGG 4 cut(s) 74, 221, 369, 859
BseMII CTCAG 3 cut(s) 137, 731, 969
BseNI ACTGG 2 cut(s) 460, 527
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 3 cut(s) 11, 145, 148
BshFI GGCC 3 cut(s) 327, 373, 690
BshNI GGYRCC 2 cut(s) 32, 44
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 4 cut(s) 74, 221, 369, 859
BsmAI GTCTC 2 cut(s) 4, 250
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 3 cut(s) 327, 373, 690
Bso31I GGTCTC 1 cut(s) 4
Bsp143I GATC 5 cut(s) 58, 217, 394, 584, 799
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 4 cut(s) 36, 262, 491, 662
BspANI GGCC 3 cut(s) 327, 373, 690
BspCNI CTCAG 3 cut(s) 136, 730, 970
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 6 cut(s) 34, 46, 183, 586, 689, 801
BspMI ACCTGC 1 cut(s) 757
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 4 cut(s) 579, 592, 794, 807
BspT107I GGYRCC 2 cut(s) 32, 44
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 2 cut(s) 460, 527
BssECI CCNNGG 6 cut(s) 39, 245, 427, 428, 809, 853
BssMI GATC 5 cut(s) 58, 217, 394, 584, 799
BssNAI GTATAC 1 cut(s) 465
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 2 cut(s) 39, 245
Bst1107I GTATAC 1 cut(s) 465
Bst2UI CCWGG 3 cut(s) 384, 429, 924
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 2 cut(s) 636, 680
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 660, 1016
BstDEI CTNAG 3 cut(s) 123, 717, 978
BstDSI CCRYGG 3 cut(s) 39, 809, 853
BstF5I GGATG 2 cut(s) 329, 727
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 47, 303
BstKTI GATC 5 cut(s) 61, 220, 397, 587, 802
BstMAI GTCTC 2 cut(s) 4, 250
BstMBI GATC 5 cut(s) 58, 217, 394, 584, 799
BstMWI GCNNNNNNNGC 5 cut(s) 30, 44, 298, 359, 655
BstNI CCWGG 3 cut(s) 384, 429, 924
BstNSI RCATGY 2 cut(s) 653, 1052
BstSCI CCNGG 4 cut(s) 67, 382, 427, 922
BstV1I GCAGC 3 cut(s) 11, 145, 148
BstX2I RGATCY 2 cut(s) 584, 799
BstYI RGATCY 2 cut(s) 584, 799
BstZ17I GTATAC 1 cut(s) 465
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 3 cut(s) 327, 373, 690
BtgI CCRYGG 3 cut(s) 39, 809, 853
BtrI CACGTC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 329, 727
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 634, 678
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 2 cut(s) 660, 1016
CciI TCATGA 1 cut(s) 478
CfoI GCGC 2 cut(s) 47, 303
Cfr13I GGNCC 4 cut(s) 83, 326, 371, 688
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 1035
CviAII CATG 7 cut(s) 40, 161, 221, 479, 650, 929, 1049
CviQI GTAC 1 cut(s) 1035
DdeI CTNAG 3 cut(s) 123, 717, 978
DinI GGCGCC 1 cut(s) 46
DpnI GATC 5 cut(s) 60, 219, 396, 586, 801
DpnII GATC 5 cut(s) 58, 217, 394, 584, 799
DrdI GACNNNNNNGTC 1 cut(s) 779
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 779
Eam1104I CTCTTC 2 cut(s) 636, 680
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 2 cut(s) 636, 680
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 39, 245
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 278
Eco81I CCTNAGG 1 cut(s) 717
EcoRII CCWGG 3 cut(s) 382, 427, 922
EcoT14I CCWWGG 2 cut(s) 39, 245
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 2 cut(s) 39, 245
FaeI CATG 7 cut(s) 43, 164, 224, 482, 653, 932, 1052
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FaqI GGGAC 1 cut(s) 51
FatI CATG 7 cut(s) 39, 160, 220, 478, 649, 928, 1048
FbaI TGATCA 2 cut(s) 217, 394
FblI GTMKAC 3 cut(s) 411, 464, 771
Fnu4HI GCNGC 3 cut(s) 25, 134, 137
FokI GGATG 2 cut(s) 316, 734
Fsp4HI GCNGC 3 cut(s) 25, 134, 137
FspBI CTAG 2 cut(s) 503, 659
GlaI GCGC 2 cut(s) 46, 302
GluI GCNGC 3 cut(s) 25, 134, 137
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 3 cut(s) 327, 373, 690
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 30
HhaI GCGC 2 cut(s) 47, 303
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 7 cut(s) 43, 164, 224, 482, 653, 932, 1052
Hin6I GCGC 2 cut(s) 45, 301
HinP1I GCGC 2 cut(s) 45, 301
HinfI GANTC 3 cut(s) 78, 258, 883
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 705, 839
Hpy166II GTNNAC 3 cut(s) 412, 465, 772
Hpy188I TCNGA 9 cut(s) 77, 83, 98, 126, 297, 565, 584, 799, 979
Hpy188III TCNNGA 7 cut(s) 215, 398, 479, 503, 719, 895, 998
Hpy8I GTNNAC 3 cut(s) 412, 465, 772
Hpy99I CGWCG 2 cut(s) 21, 24
HpyAV CCTTC 2 cut(s) 23, 1048
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 6 cut(s) 7, 649, 710, 910, 957, 1014
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 44, 298, 359, 655
HpyF3I CTNAG 3 cut(s) 123, 717, 978
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 7 cut(s) 43, 164, 224, 482, 653, 932, 1052
HspAI GCGC 2 cut(s) 45, 301
KasI GGCGCC 1 cut(s) 44
Ksp22I TGATCA 2 cut(s) 217, 394
Kzo9I GATC 5 cut(s) 58, 217, 394, 584, 799
LmnI GCTCC 3 cut(s) 181, 613, 1045
Lsp1109I GCAGC 3 cut(s) 11, 145, 148
LweI GCATC 2 cut(s) 288, 719
MaeI CTAG 2 cut(s) 503, 659
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 249, 529
MalI GATC 5 cut(s) 60, 219, 396, 586, 801
MboI GATC 5 cut(s) 58, 217, 394, 584, 799
MboII GAAGA 5 cut(s) 53, 552, 653, 667, 677
MflI RGATCY 2 cut(s) 584, 799
MluCI AATT 7 cut(s) 387, 734, 841, 916, 940, 1009, 1061
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 2 cut(s) 252, 877
MmeI TCCRAC 2 cut(s) 174, 562
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 38
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 4 cut(s) 69, 384, 429, 924
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 3 cut(s) 384, 429, 924
MwoI GCNNNNNNNGC 5 cut(s) 30, 44, 298, 359, 655
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 69
NcoI CCATGG 1 cut(s) 39
NdeII GATC 5 cut(s) 58, 217, 394, 584, 799
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 7 cut(s) 43, 164, 224, 482, 653, 932, 1052
NlaIV GGNNCC 6 cut(s) 34, 46, 183, 586, 689, 801
NmuCI GTSAC 1 cut(s) 249
NspI RCATGY 2 cut(s) 653, 1052
PagI TCATGA 1 cut(s) 478
PasI CCCWGGG 1 cut(s) 428
PctI GAATGC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 382
PkrI GCNGC 3 cut(s) 26, 135, 138
PleI GAGTC 2 cut(s) 252, 877
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 2 cut(s) 252, 877
Psp6I CCWGG 3 cut(s) 382, 427, 922
PspGI CCWGG 3 cut(s) 382, 427, 922
PspN4I GGNNCC 6 cut(s) 34, 46, 183, 586, 689, 801
PspPI GGNCC 4 cut(s) 83, 326, 371, 688
PsuI RGATCY 2 cut(s) 584, 799
RsaI GTAC 1 cut(s) 1036
RsaNI GTAC 1 cut(s) 1035
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 25, 134, 137
Sau3AI GATC 5 cut(s) 58, 217, 394, 584, 799
Sau96I GGNCC 4 cut(s) 83, 326, 371, 688
SchI GAGTC 2 cut(s) 252, 877
ScrFI CCNGG 4 cut(s) 69, 384, 429, 924
SfaNI GCATC 2 cut(s) 288, 719
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 7 cut(s) 387, 734, 841, 916, 940, 1009, 1061
SsiI CCGC 4 cut(s) 36, 262, 491, 662
SspDI GGCGCC 1 cut(s) 44
SspI AATATT 2 cut(s) 276, 697
SspMI CTAG 2 cut(s) 503, 659
StyD4I CCNGG 4 cut(s) 67, 382, 427, 922
StyI CCWWGG 2 cut(s) 39, 245
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 57
TasI AATT 7 cut(s) 387, 734, 841, 916, 940, 1009, 1061
TatI WGTACW 1 cut(s) 1034
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 2 cut(s) 634, 685
TseFI GTSAC 1 cut(s) 249
TseI GCWGC 3 cut(s) 24, 133, 136
Tsp45I GTSAC 1 cut(s) 249
TspDTI ATGAA 3 cut(s) 149, 467, 776
TspGWI ACGGA 1 cut(s) 826
TspRI CASTG 2 cut(s) 634, 685
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 83
XapI RAATTY 1 cut(s) 387
XbaI TCTAGA 1 cut(s) 502
XceI RCATGY 2 cut(s) 653, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 1038
XmiI GTMKAC 3 cut(s) 411, 464, 771
XspI CTAG 2 cut(s) 503, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.