pycom15g22420

Uncharacterised protein family (UPF0261)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008344
Physical Location & Seq
Reverse (-)
16557619 .. 16561166
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2265 bp
ATGCCGGCCGGTAATAAAAACTCCCGAGTTTTCTGTATCGGAACCGTCGATACGAAGCTCGACGAGCTCCGATTCCTCGCCGAGTCGGTTCGATCCCATCTCAACGCCTTCTCCACAGCTTCCTTACTGAAGGTCAAAGTATCGGTGGTGGACGTATCGGCGAGTCGGACCGAGACCGATAGAAATCCAACCAATTTCGAATTCGTTTCGAGGAAGGAGATTCTCAGTCACAGTTCTGAAGCATCAGCCGATCAGATTCCGGAGGACAGGGGCGAAGCGGTTGCTGTAATGAGCAAAGCGCTCGAGAACTTCCTGTCCAAAGCTCAGAACGACGGCATTTTAGCCGGAGCTGTAGGTCTCGGAGGCAGCGGAGGCACGGCACTGATATCCTCCGCCCTAAGATCTCTGCCAATCGGAGTACCGAAGCTGATCTTATCGACCGTCGCGAGCGGGCGGACCGATCACTATGTGGGGACTTCGGACCTGGTGTTGTACCCGTCAATTGTCGATGTCTGTGGGATTAATAGCGTGAGCAGGGTCGTATTGAGCAATGCTGCTGCTGCGTTCTGTGGGATGGTGGTTGGGGGAGTTGGGAGGGGCGGAGATTCACGCGGTGGCGGTGGAGGGGTGGAGAAACCGACGGTGGGGCTGACCATGTTTGGGGTGACGACTCAGTGTGTGAATGGGGTTAAAGAGAGATTGGAGAAGCAGGGGTATGAGACATTGGTTTTTCATGCCACTGGGGTTGGAGGGAGAGCCATGGAGTCTCTGGTTAGAGGTGGATTCATAAAGGGGGTTTTGGATATCACAACAACAGAGGTTGCAGACCATGTGGTAGGGGGTGTCATGGCTTGCGATGCTTCCCGCTTTGATGCCATCATAGAGAAAAAGATTCCTTTAGTACTAAGTGTTGGAGCTTTGGATATGGTAAATTTTGGAGCTAAGGATACCATACCATCCAATTTTCAACACAGAAAGATTCATGAACATAATAAGCAGGTTTCACTCATGAGAACCACTGTAGATGAGAACAAGAAATTTGCTGGTTTTATAGCAGATAAGTTGAACAAATCATCATCAAAGGTGGTTGTTTGCCTACCGCAAAAGGGTATCTCTGCTTTGGATGCACCGGGGCAGCCTTTCCATGATCCAGAGGTCAATGCTTCGCTGATTAATGAACTACAGAGGCTGGTCCAGACAAATGAAGATCGGAAGGTAAGGGTGTACCCTCATCATATAAATGATCCTGAGTTTGCAAATGCATTGGTGGACTCATTTTTAGAGATTAGTACAAAGAGTTCTATCCAGTCTACTCCACCACAAGTTCATGAATCAAACCAACAAGTTCATGAAAGTTCTGTTTCGAAGATAAATTTGTCAAGCTCTGAGACCGTTCCACGTAACCTGAGTGATTTCCCAGATGCAAGACCAGAAACTCTGGAAAGAACTAGGGTGGTGTTGCAGCAACTCAAAGATCAAATAAATAGAGGAATTCCAATTATAGGGGCAGGTGCAGGGACAGGCATATCTGCAAAGTTTGAGGAAGCTGGTGGTGTTGATCTGATAGTGGTGTACAATTCAGGGCGCTTTCGCATGGCAGGAAGAGGTTCATTGGCAGGCTTATTGCCGTTTGCTGATGCAAATGCTGTAGTACTTGACATGGCCAATGAAGTTTTGCCAGTGGTGAAGAAGGTACCTGTTCTTGCTGGGGTGTGTGGCACCGATCCTTTTCGCCGAATGGATTTCTTTTTAAGGCAGGTGGAGTCAATTGGATTCTCTGGTGTCCAAAATTTTCCAACAGTTGGACTCTTTGATGGTAATTTTAGACAAAATCTTGAAGAAACTGGAATGGGATACCGATTGGAGGTTGAGATGATCGAAAAAGCACATAAGATGGGGCTCTTGACAACCCCATATGCTTTCAATCAAGATGAAGCTGTGCAAATGGCAAAAGCTGGTGCTGATATCATTGTGGCTCATATGGGACTCACCACATCCGGCTCTATAGGGGCAAAGACGGCTGTTTCACTGGATGAAAGTGTAGATCGTGTACAAAATATTGCAGATGCTGCAAGTAGGATCAATCCCAACGCCATTGTGCTTTGCCATGGTGGTCCAATCTCGGGTCCAAGAGAGGCAAAATTTATACTAAAGAACACCAAGGGAGTTCATGGGTTTTATGGGGCGTCAAGCGTGGAGAGGCTACCTGTTGAACAGGCTATCACCTCCACGGTCCAACAGTACAAATCGATTTCCATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_hydrolase UPF0261

Protein Analysis

755

Amino Acids

80.34

Weight (kDa)

6.52

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0261 PF06792 9 - 194 6.5e-55 Uncharacterised protein family (UPF0261) N-terminal domain
UPF0261_C PF23189 212 - 429 1.4e-95 Uncharacterised protein family (UPF0261) C-terminal domain
PEP_hydrolase PF09370 486 - 751 1.2e-131 Phosphoenolpyruvate hydrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1499, 1747
Acc36I ACCTGC 3 cut(s) 988, 1499, 1747
Acc65I GGTACC 1 cut(s) 1693
AccB1I GGYRCC 2 cut(s) 1693, 1718
AccB7I CCANNNNNTGG 1 cut(s) 1802
AccBSI CCGCTC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 1310
AccII CGCG 2 cut(s) 446, 612
AccIII TCCGGA 1 cut(s) 259
AclWI GGATC 5 cut(s) 87, 1142, 1238, 1718, 2087
AcoI YGGCCR 2 cut(s) 6, 1662
AcsI RAATTY 7 cut(s) 200, 931, 1037, 1372, 1491, 1789, 2141
AcuI CTGAAG 2 cut(s) 149, 258
AcyI GRCGYC 1 cut(s) 2186
AdeI CACNNNGTG 2 cut(s) 469, 614
AfeI AGCGCT 1 cut(s) 300
AfiI CCNNNNNNNGG 7 cut(s) 644, 660, 1106, 1502, 1802, 1864, 2123
AgsI TTSAA 5 cut(s) 968, 1066, 1838, 1924, 2213
AjnI CCWGG 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 5 cut(s) 167, 362, 713, 771, 1382
AlwI GGATC 5 cut(s) 87, 1142, 1238, 1718, 2087
AlwNI CAGNNNCTG 2 cut(s) 1189, 2069
Ama87I CYCGRG 3 cut(s) 24, 302, 2122
Aor13HI TCCGGA 1 cut(s) 259
Aor51HI AGCGCT 1 cut(s) 300
AoxI GGCC 2 cut(s) 6, 1662
ApeKI GCWGC 7 cut(s) 366, 554, 557, 560, 1135, 1462, 2069
ApoI RAATTY 7 cut(s) 200, 931, 1037, 1372, 1491, 1789, 2141
AseI ATTAAT 2 cut(s) 522, 1173
Asp700I GAANNNNTTC 1 cut(s) 1606
Asp718I GGTACC 1 cut(s) 1693
AspLEI GCGC 2 cut(s) 301, 1587
AspS9I GGNCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
AsuC2I CCSGG 1 cut(s) 1131
AsuHPI GGTGA 4 cut(s) 676, 1696, 1981, 2215
AsuII TTCGAA 2 cut(s) 198, 1364
AvaI CYCGRG 3 cut(s) 24, 302, 2122
AvaII GGWCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
BalI TGGCCA 1 cut(s) 1664
BanI GGYRCC 2 cut(s) 1693, 1718
BanII GRGCYC 2 cut(s) 69, 1902
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 7 cut(s) 378, 541, 544, 547, 1147, 1474, 2056
BccI CCATC 7 cut(s) 105, 568, 884, 964, 1808, 1888, 2264
BceAI ACGGC 4 cut(s) 349, 393, 1612, 2034
BcgI CGANNNNNNTGC 4 cut(s) 263, 283, 297, 317
BciT130I CCWGG 1 cut(s) 485
BciVI GTATCC 2 cut(s) 940, 1847
BcnI CCSGG 1 cut(s) 1131
BcoDI GTCTC 5 cut(s) 167, 362, 713, 771, 1382
BfaI CTAG 1 cut(s) 1449
BfmI CTRYAG 5 cut(s) 351, 1020, 1181, 1647, 2004
BfoI RGCGCY 2 cut(s) 302, 1588
BfuAI ACCTGC 3 cut(s) 988, 1499, 1747
BfuI GTATCC 2 cut(s) 940, 1847
BglII AGATCT 1 cut(s) 401
BisI GCNGC 7 cut(s) 367, 555, 558, 561, 1136, 1463, 2070
BlsI GCNGC 7 cut(s) 368, 556, 559, 562, 1137, 1464, 2071
BmcAI AGTACT 2 cut(s) 903, 1653
Bme1390I CCNGG 2 cut(s) 485, 1131
Bme18I GGWCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
BmeT110I CYCGRG 3 cut(s) 24, 302, 2122
BmgT120I GGNCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
BmiI GGNNCC 4 cut(s) 43, 1695, 1720, 2127
BmrFI CCNGG 2 cut(s) 485, 1131
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 7 cut(s) 251, 847, 862, 1114, 1411, 1627, 2056
BmuI ACTGGG 1 cut(s) 750
Bpu10I CCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 2 cut(s) 198, 1364
BpuMI CCSGG 1 cut(s) 1131
Bsa29I ATCGAT 1 cut(s) 2249
BsaAI YACGTR 1 cut(s) 1400
BsaBI GATNNNNATC 2 cut(s) 183, 2255
BsaHI GRCGYC 1 cut(s) 2186
BsaI GGTCTC 3 cut(s) 167, 362, 1382
BsaJI CCNNGG 5 cut(s) 759, 1130, 2107, 2160, 2229
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 7 cut(s) 644, 660, 1106, 1502, 1802, 1864, 2123
Bse118I RCCGGY 2 cut(s) 4, 8
Bse1I ACTGG 5 cut(s) 745, 1306, 1679, 1849, 2034
Bse3DI GCAATG 1 cut(s) 556
Bse8I GATNNNNATC 2 cut(s) 183, 2255
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 1 cut(s) 485
BseCI ATCGAT 1 cut(s) 2249
BseDI CCNNGG 5 cut(s) 759, 1130, 2107, 2160, 2229
BseGI GGATG 5 cut(s) 579, 956, 1129, 1994, 2038
BseJI GATNNNNATC 2 cut(s) 183, 2255
BseLI CCNNNNNNNGG 7 cut(s) 644, 660, 1106, 1502, 1802, 1864, 2123
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 6 cut(s) 238, 338, 686, 1239, 1377, 1397
BseNI ACTGG 5 cut(s) 745, 1306, 1679, 1849, 2034
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 7 cut(s) 378, 541, 544, 547, 1147, 1474, 2056
BseYI CCCAGC 1 cut(s) 1706
BsgI GTGCAG 1 cut(s) 1533
Bsh1236I CGCG 2 cut(s) 446, 612
Bsh1285I CGRYCG 2 cut(s) 9, 441
BshFI GGCC 2 cut(s) 8, 1664
BshNI GGYRCC 2 cut(s) 1693, 1718
BshVI ATCGAT 1 cut(s) 2249
BsiEI CGRYCG 2 cut(s) 9, 441
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 3 cut(s) 24, 302, 2122
BsiSI CCGG 6 cut(s) 5, 9, 260, 345, 1130, 1998
BslFI GGGAC 3 cut(s) 487, 1531, 1998
BslI CCNNNNNNNGG 7 cut(s) 644, 660, 1106, 1502, 1802, 1864, 2123
BsmAI GTCTC 5 cut(s) 167, 362, 713, 771, 1382
BsmFI GGGAC 3 cut(s) 487, 1531, 1998
BsnI GGCC 2 cut(s) 8, 1664
Bso31I GGTCTC 3 cut(s) 167, 362, 1382
BsoBI CYCGRG 3 cut(s) 24, 302, 2122
Bsp119I TTCGAA 2 cut(s) 198, 1364
Bsp1286I GDGCHC 2 cut(s) 69, 1902
Bsp13I TCCGGA 1 cut(s) 259
Bsp1407I TGTACA 2 cut(s) 1572, 2050
Bsp19I CCATGG 2 cut(s) 759, 2107
Bsp68I TCGCGA 1 cut(s) 446
BspANI GGCC 2 cut(s) 8, 1664
BspCNI CTCAG 6 cut(s) 237, 337, 685, 1240, 1378, 1398
BspDI ATCGAT 1 cut(s) 2249
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 2 cut(s) 446, 612
BspHI TCATGA 4 cut(s) 982, 1008, 1327, 1348
BspLI GGNNCC 4 cut(s) 43, 1695, 1720, 2127
BspMI ACCTGC 3 cut(s) 988, 1499, 1747
BspPI GGATC 5 cut(s) 87, 1142, 1238, 1718, 2087
BspT104I TTCGAA 2 cut(s) 198, 1364
BspT107I GGYRCC 2 cut(s) 1693, 1718
BspTNI GGTCTC 3 cut(s) 167, 362, 1382
BsrBI CCGCTC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 2 cut(s) 4, 8
BsrGI TGTACA 2 cut(s) 1572, 2050
BsrI ACTGG 5 cut(s) 745, 1306, 1679, 1849, 2034
BssAI RCCGGY 2 cut(s) 4, 8
BssECI CCNNGG 5 cut(s) 759, 1130, 2107, 2160, 2229
BssNI GRCGYC 1 cut(s) 2186
BssT1I CCWWGG 3 cut(s) 759, 2107, 2160
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 9 cut(s) 46, 233, 442, 643, 1021, 1393, 1801, 2233, 2241
Bst6I CTCTTC 1 cut(s) 1597
BstACI GRCGYC 1 cut(s) 2186
BstAPI GCANNNNNTGC 1 cut(s) 2069
BstAUI TGTACA 2 cut(s) 1572, 2050
BstBAI YACGTR 1 cut(s) 1400
BstBI TTCGAA 2 cut(s) 198, 1364
BstC8I GCNNGC 5 cut(s) 6, 448, 452, 853, 1618
BstDEI CTNAG 9 cut(s) 224, 324, 398, 672, 905, 942, 1248, 1386, 1406
BstDSI CCRYGG 3 cut(s) 759, 2107, 2229
BstF5I GGATG 5 cut(s) 579, 956, 1129, 1994, 2038
BstFNI CGCG 2 cut(s) 446, 612
BstH2I RGCGCY 2 cut(s) 302, 1588
BstHHI GCGC 2 cut(s) 301, 1587
BstMAI GTCTC 5 cut(s) 167, 362, 713, 771, 1382
BstMCI CGRYCG 2 cut(s) 9, 441
BstMWI GCNNNNNNNGC 7 cut(s) 64, 372, 560, 857, 1124, 2018, 2069
BstNI CCWGG 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 483, 1129
BstSFI CTRYAG 5 cut(s) 351, 1020, 1181, 1647, 2004
BstUI CGCG 2 cut(s) 446, 612
BstV1I GCAGC 7 cut(s) 378, 541, 544, 547, 1147, 1474, 2056
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 2249
BsuI GTATCC 2 cut(s) 940, 1847
BsuRI GGCC 2 cut(s) 8, 1664
BsuTUI ATCGAT 1 cut(s) 2249
BtgI CCRYGG 3 cut(s) 759, 2107, 2229
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 5 cut(s) 579, 956, 1129, 1994, 2038
BtsIMutI CAGTG 6 cut(s) 380, 680, 738, 1017, 1686, 2027
BtuMI TCGCGA 1 cut(s) 446
BveI ACCTGC 3 cut(s) 988, 1499, 1747
Cac8I GCNNGC 5 cut(s) 6, 448, 452, 853, 1618
CaiI CAGNNNCTG 2 cut(s) 1189, 2069
CciI TCATGA 4 cut(s) 982, 1008, 1327, 1348
CfoI GCGC 2 cut(s) 301, 1587
Cfr10I RCCGGY 2 cut(s) 4, 8
Cfr13I GGNCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
ClaI ATCGAT 1 cut(s) 2249
CpoI CGGWCCG 2 cut(s) 168, 456
CseI GACGC 1 cut(s) 2175
CsiI ACCWGGT 1 cut(s) 483
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CspI CGGWCCG 2 cut(s) 168, 456
DdeI CTNAG 9 cut(s) 224, 324, 398, 672, 905, 942, 1248, 1386, 1406
DraIII CACNNNGTG 2 cut(s) 469, 614
EaeI YGGCCR 2 cut(s) 6, 1662
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 1597
EarI CTCTTC 1 cut(s) 1597
EciI GGCGGA 3 cut(s) 382, 469, 615
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 759, 2107, 2160
Eco24I GRGCYC 2 cut(s) 69, 1902
Eco31I GGTCTC 3 cut(s) 167, 362, 1382
Eco32I GATATC 3 cut(s) 387, 805, 1966
Eco47I GGWCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
Eco47III AGCGCT 1 cut(s) 300
Eco52I CGGCCG 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 149, 258
Eco88I CYCGRG 3 cut(s) 24, 302, 2122
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 2 cut(s) 200, 1491
EcoRII CCWGG 1 cut(s) 483
EcoRV GATATC 3 cut(s) 387, 805, 1966
EcoT14I CCWWGG 3 cut(s) 759, 2107, 2160
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 2 cut(s) 69, 1902
ErhI CCWWGG 3 cut(s) 759, 2107, 2160
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 3 cut(s) 487, 1531, 1998
FauI CCCGC 2 cut(s) 443, 872
FauNDI CATATG 2 cut(s) 1915, 1980
FblI GTMKAC 1 cut(s) 1310
Fnu4HI GCNGC 7 cut(s) 367, 555, 558, 561, 1136, 1463, 2070
FokI GGATG 5 cut(s) 586, 943, 1136, 1981, 2045
FriOI GRGCYC 2 cut(s) 69, 1902
Fsp4HI GCNGC 7 cut(s) 367, 555, 558, 561, 1136, 1463, 2070
FspBI CTAG 1 cut(s) 1449
GlaI GCGC 2 cut(s) 300, 1586
GluI GCNGC 7 cut(s) 367, 555, 558, 561, 1136, 1463, 2070
GsaI CCCAGC 1 cut(s) 1710
HaeII RGCGCY 2 cut(s) 302, 1588
HaeIII GGCC 2 cut(s) 8, 1664
HapII CCGG 6 cut(s) 5, 9, 260, 345, 1130, 1998
HgaI GACGC 1 cut(s) 2175
HhaI GCGC 2 cut(s) 301, 1587
Hin1I GRCGYC 1 cut(s) 2186
Hin6I GCGC 2 cut(s) 299, 1585
HinP1I GCGC 2 cut(s) 299, 1585
HpaII CCGG 6 cut(s) 5, 9, 260, 345, 1130, 1998
HphI GGTGA 4 cut(s) 676, 1696, 1981, 2215
Hpy166II GTNNAC 6 cut(s) 151, 1225, 1270, 1311, 1573, 2051
Hpy8I GTNNAC 6 cut(s) 151, 1225, 1270, 1311, 1573, 2051
Hpy99I CGWCG 5 cut(s) 50, 65, 335, 446, 643
HpyAV CCTTC 5 cut(s) 118, 124, 208, 1207, 1684
HpyCH4III ACNGT 9 cut(s) 46, 233, 442, 643, 1021, 1393, 1801, 2233, 2241
HpyCH4IV ACGT 2 cut(s) 153, 1399
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 372, 560, 857, 1124, 2018, 2069
HpyF3I CTNAG 9 cut(s) 224, 324, 398, 672, 905, 942, 1248, 1386, 1406
HpySE526I ACGT 2 cut(s) 153, 1399
Hsp92I GRCGYC 1 cut(s) 2186
HspAI GCGC 2 cut(s) 299, 1585
Kpn2I TCCGGA 1 cut(s) 259
KpnI GGTACC 1 cut(s) 1697
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 72, 347, 914, 938
Lsp1109I GCAGC 7 cut(s) 378, 541, 544, 547, 1147, 1474, 2056
LweI GCATC 7 cut(s) 251, 847, 862, 1114, 1411, 1627, 2056
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 1449
MaeII ACGT 2 cut(s) 153, 1399
MaeIII GTNAC 3 cut(s) 227, 664, 1400
MbiI CCGCTC 1 cut(s) 450
MboII GAAGA 5 cut(s) 1217, 1378, 1614, 1699, 1850
MfeI CAATTG 2 cut(s) 501, 1767
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 69, 1902
MlsI TGGCCA 1 cut(s) 1664
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 8 cut(s) 92, 172, 664, 773, 1265, 1772, 1800, 1980
MmeI TCCRAC 7 cut(s) 146, 212, 727, 892, 1783, 1820, 2260
Mox20I TGGCCA 1 cut(s) 1664
Mph1103I ATGCAT 1 cut(s) 1264
MroI TCCGGA 1 cut(s) 259
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 1606
MscI TGGCCA 1 cut(s) 1664
MseI TTAA 4 cut(s) 522, 690, 1173, 1751
MslI CAYNNNNRTG 1 cut(s) 1239
Msp20I TGGCCA 1 cut(s) 1664
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 6 cut(s) 5, 9, 260, 345, 1130, 1998
MspR9I CCNGG 2 cut(s) 485, 1131
MunI CAATTG 2 cut(s) 501, 1767
MvaI CCWGG 1 cut(s) 485
MvnI CGCG 2 cut(s) 446, 612
MwoI GCNNNNNNNGC 7 cut(s) 64, 372, 560, 857, 1124, 2018, 2069
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 1131
NcoI CCATGG 2 cut(s) 759, 2107
NdeI CATATG 2 cut(s) 1915, 1980
NgoMIV GCCGGC 1 cut(s) 4
NlaIV GGNNCC 4 cut(s) 43, 1695, 1720, 2127
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 2 cut(s) 227, 664
NruI TCGCGA 1 cut(s) 446
NsiI ATGCAT 1 cut(s) 1264
NspV TTCGAA 2 cut(s) 198, 1364
PaeR7I CTCGAG 1 cut(s) 302
PagI TCATGA 4 cut(s) 982, 1008, 1327, 1348
PaqCI CACCTGC 2 cut(s) 1499, 1747
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 443
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 1606
PfeI GAWTC 9 cut(s) 72, 220, 256, 605, 783, 892, 979, 1331, 1773
PflMI CCANNNNNTGG 1 cut(s) 1802
PkrI GCNGC 7 cut(s) 368, 556, 559, 562, 1137, 1464, 2071
PleI GAGTC 8 cut(s) 91, 171, 664, 772, 1265, 1771, 1800, 1980
PpsI GAGTC 8 cut(s) 91, 171, 664, 772, 1265, 1771, 1800, 1980
Ppu21I YACGTR 1 cut(s) 1400
PshBI ATTAAT 2 cut(s) 522, 1173
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 483
PspFI CCCAGC 1 cut(s) 1706
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 4 cut(s) 43, 1695, 1720, 2127
PspPI GGNCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
PsrI GAACNNNNNNTAC 4 cut(s) 34, 66, 1282, 1314
PstNI CAGNNNCTG 2 cut(s) 1189, 2069
PsuI RGATCY 1 cut(s) 401
RruI TCGCGA 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 1239
Rsr2I CGGWCCG 2 cut(s) 168, 456
RsrII CGGWCCG 2 cut(s) 168, 456
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 4 cut(s) 522, 690, 1173, 1751
SatI GCNGC 7 cut(s) 367, 555, 558, 561, 1136, 1463, 2070
Sau96I GGNCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
ScaI AGTACT 2 cut(s) 903, 1653
SchI GAGTC 8 cut(s) 92, 172, 664, 773, 1265, 1772, 1800, 1980
ScrFI CCNGG 2 cut(s) 485, 1131
SduI GDGCHC 2 cut(s) 69, 1902
SexAI ACCWGGT 1 cut(s) 483
SfaNI GCATC 7 cut(s) 251, 847, 862, 1114, 1411, 1627, 2056
SfcI CTRYAG 5 cut(s) 351, 1020, 1181, 1647, 2004
Sfr274I CTCGAG 1 cut(s) 302
SfuI TTCGAA 2 cut(s) 198, 1364
SinI GGWCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SspI AATATT 1 cut(s) 2059
SspMI CTAG 1 cut(s) 1449
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 483, 1129
StyI CCWWGG 3 cut(s) 759, 2107, 2160
TaaI ACNGT 9 cut(s) 46, 233, 442, 643, 1021, 1393, 1801, 2233, 2241
TaiI ACGT 2 cut(s) 156, 1402
TaqII GACCGA 3 cut(s) 185, 191, 473
TatI WGTACW 6 cut(s) 901, 1289, 1572, 1651, 2050, 2241
TfiI GAWTC 9 cut(s) 72, 220, 256, 605, 783, 892, 979, 1331, 1773
Tru1I TTAA 4 cut(s) 522, 690, 1173, 1751
Tru9I TTAA 4 cut(s) 522, 690, 1173, 1751
TscAI CASTG 6 cut(s) 387, 680, 745, 1024, 1686, 2034
TseFI GTSAC 2 cut(s) 227, 664
TseI GCWGC 7 cut(s) 366, 554, 557, 560, 1135, 1462, 2069
Tsp45I GTSAC 2 cut(s) 227, 664
TspRI CASTG 6 cut(s) 387, 680, 745, 1024, 1686, 2034
Van91I CCANNNNNTGG 1 cut(s) 1802
VpaK11BI GGWCC 7 cut(s) 168, 456, 481, 1192, 2114, 2126, 2233
VspI ATTAAT 2 cut(s) 522, 1173
XapI RAATTY 7 cut(s) 200, 931, 1037, 1372, 1491, 1789, 2141
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 1310
XmnI GAANNNNTTC 1 cut(s) 1606
XspI CTAG 1 cut(s) 1449
ZrmI AGTACT 2 cut(s) 903, 1653
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.