pycom15g23430

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005012
Physical Location & Seq
Reverse (-)
17523969 .. 17525817
1849 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGCCTCTCTCTTATTGTGTAGCGGAGAACAAACCCTGCGCGCGGTGGGTTCAGAAGTATTTCAAGGACTGCCTTTGCAATCTCAACGATGACATCTCGTTTGCTTTTGGTCTGATCAGCTTGGTGTGTTGGGGGGTGGCTGAAGTTCCTCAAATCATCACAAACTTTCACACCAAGTCCAGCCACGGAGTCTCCCTCGCCTTCCTTTGCACTTGGGTCGCCGGGGATGTGTTTAATTTATTGGGTTGTTTTCTGGAACCAGCAACGGATGAAGAAGAGAATAAAAAACCATTGAATCCAAAGTTGGCTGATTCAGGCATTCCAATACCAAGTGCTTCACCCAAAGCCACTCCTAGAAGGGAGTTCTACTATACGTCAGCGAGATCTTTGGCTGGCAGTGGTACTCCACCGTTTCGATCGTATATGAGGGCAGCTAGAAGTGGCCCTTCTGCAATGACAATAGACAGTGACTCATCTTCTGAGGATGAGTCAGCTCCGGTTACATCTAACAAGAACTCTGTTACCCAGCCTAGGCCAATCCCAAGATTGGTGAGCCTCACAAATCTAGTTTCCGACTTATGGTCATTTTATACATTGCTTAAAACTGTTCACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

206

Amino Acids

22.52

Weight (kDa)

6.81

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 32 - 87 1.9e-18 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 43
AciI CCGC 2 cut(s) 23, 43
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 4 cut(s) 42, 531, 547, 579
AgsI TTSAA 2 cut(s) 64, 295
AhdI GACNNNNNGTC 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 3 cut(s) 120, 434, 494
AluI AGCT 3 cut(s) 120, 434, 494
Alw26I GTCTC 1 cut(s) 196
AoxI GGCC 2 cut(s) 442, 533
ApeKI GCWGC 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
Asp700I GAANNNNTTC 1 cut(s) 59
AspA2I CCTAGG 1 cut(s) 530
AspLEI GCGC 2 cut(s) 41, 43
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 330, 562, 602
AvrII CCTAGG 1 cut(s) 530
BbvI GCAGC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 4 cut(s) 354, 435, 531, 566
BglII AGATCT 1 cut(s) 383
BisI GCNGC 1 cut(s) 432
BlnI CCTAGG 1 cut(s) 530
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 1 cut(s) 223
BmeRI GACNNNNNGTC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 223
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpuMI CCSGG 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 184, 222, 530
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 531, 547, 579
Bse3DI GCAATG 2 cut(s) 459, 593
BseDI CCNNGG 3 cut(s) 184, 222, 530
BseGI GGATG 3 cut(s) 232, 274, 490
BseLI CCNNNNNNNGG 4 cut(s) 42, 531, 547, 579
BseMI GCAATG 2 cut(s) 459, 593
BseMII CTCAG 1 cut(s) 471
BsePI GCGCGC 1 cut(s) 39
BseXI GCAGC 1 cut(s) 443
BseYI CCCAGC 1 cut(s) 525
Bsh1236I CGCG 2 cut(s) 41, 43
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 2 cut(s) 444, 535
BsiEI CGRYCG 1 cut(s) 419
BsiSI CCGG 2 cut(s) 222, 497
BslI CCNNNNNNNGG 4 cut(s) 42, 531, 547, 579
BsmAI GTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 2 cut(s) 444, 535
Bsp143I GATC 3 cut(s) 114, 383, 416
BspACI CCGC 2 cut(s) 23, 43
BspANI GGCC 2 cut(s) 444, 535
BspCNI CTCAG 1 cut(s) 472
BspFNI CGCG 2 cut(s) 41, 43
BspLI GGNNCC 1 cut(s) 258
BsrDI GCAATG 2 cut(s) 459, 593
BssECI CCNNGG 3 cut(s) 184, 222, 530
BssHII GCGCGC 1 cut(s) 39
BssMI GATC 3 cut(s) 114, 383, 416
BssT1I CCWWGG 1 cut(s) 530
Bst4CI ACNGT 3 cut(s) 411, 467, 607
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 41, 394
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 3 cut(s) 232, 274, 490
BstFNI CGCG 2 cut(s) 41, 43
BstHHI GCGC 2 cut(s) 41, 43
BstKTI GATC 3 cut(s) 117, 386, 419
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 3 cut(s) 114, 383, 416
BstMCI CGRYCG 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 221
BstUI CGCG 2 cut(s) 41, 43
BstV1I GCAGC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 2 cut(s) 444, 535
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 3 cut(s) 232, 274, 490
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 403, 472
Cac8I GCNNGC 2 cut(s) 41, 394
CfoI GCGC 2 cut(s) 41, 43
Cfr13I GGNCC 1 cut(s) 443
Csp6I GTAC 1 cut(s) 402
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 1 cut(s) 480
DpnI GATC 3 cut(s) 116, 385, 418
DpnII GATC 3 cut(s) 114, 383, 416
DriI GACNNNNNGTC 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 270
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 530
FaiI YATR 5 cut(s) 372, 423, 425, 580, 591
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FbaI TGATCA 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 3 cut(s) 239, 281, 497
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 4 cut(s) 354, 435, 531, 566
GlaI GCGC 2 cut(s) 40, 42
GluI GCNGC 1 cut(s) 432
GsaI CCCAGC 1 cut(s) 529
HaeIII GGCC 2 cut(s) 444, 535
HapII CCGG 2 cut(s) 222, 497
HhaI GCGC 2 cut(s) 41, 43
Hin6I GCGC 2 cut(s) 39, 41
HinP1I GCGC 2 cut(s) 39, 41
HinfI GANTC 5 cut(s) 189, 295, 311, 470, 488
HpaII CCGG 2 cut(s) 222, 497
HphI GGTGA 3 cut(s) 330, 562, 602
Hpy166II GTNNAC 1 cut(s) 610
Hpy188I TCNGA 4 cut(s) 54, 114, 481, 574
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 610
HpyAV CCTTC 3 cut(s) 211, 351, 456
HpyCH4III ACNGT 3 cut(s) 411, 467, 607
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 78, 210, 452
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 1 cut(s) 374
HspAI GCGC 2 cut(s) 39, 41
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 3 cut(s) 114, 383, 416
LmnI GCTCC 1 cut(s) 499
LpnPI CCDG 9 cut(s) 49, 193, 235, 239, 273, 300, 378, 510, 539
Lsp1109I GCAGC 1 cut(s) 443
MaeI CTAG 4 cut(s) 354, 435, 531, 566
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 3 cut(s) 467, 499, 520
MalI GATC 3 cut(s) 116, 385, 418
MboI GATC 3 cut(s) 114, 383, 416
MboII GAAGA 3 cut(s) 284, 287, 468
MflI RGATCY 1 cut(s) 383
MluCI AATT 1 cut(s) 235
MlyI GAGTC 3 cut(s) 198, 464, 497
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 6 cut(s) 15, 159, 206, 420, 475, 566
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 3 cut(s) 234, 600, 616
MspI CCGG 2 cut(s) 222, 497
MspR9I CCNGG 1 cut(s) 223
Mva1269I GAATGC 1 cut(s) 318
MvnI CGCG 2 cut(s) 41, 43
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 114, 383, 416
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 467
PauI GCGCGC 1 cut(s) 39
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 295, 311
PkrI GCNGC 1 cut(s) 433
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 3 cut(s) 197, 464, 496
PpsI GAGTC 3 cut(s) 197, 464, 496
PspFI CCCAGC 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 443
PsuI RGATCY 1 cut(s) 383
PteI GCGCGC 1 cut(s) 39
PvuI CGATCG 1 cut(s) 419
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
SaqAI TTAA 3 cut(s) 234, 600, 616
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 3 cut(s) 114, 383, 416
Sau96I GGNCC 1 cut(s) 443
SchI GAGTC 3 cut(s) 198, 464, 497
ScrFI CCNGG 1 cut(s) 223
SetI ASST 5 cut(s) 122, 377, 436, 496, 615
Sse9I AATT 1 cut(s) 235
SsiI CCGC 2 cut(s) 23, 43
SspMI CTAG 4 cut(s) 354, 435, 531, 566
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 3 cut(s) 411, 467, 607
TaiI ACGT 1 cut(s) 377
TaqI TCGA 1 cut(s) 415
TasI AATT 1 cut(s) 235
TfiI GAWTC 2 cut(s) 295, 311
Tru1I TTAA 3 cut(s) 234, 600, 616
Tru9I TTAA 3 cut(s) 234, 600, 616
TscAI CASTG 2 cut(s) 403, 472
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 2 cut(s) 201, 281
TspRI CASTG 2 cut(s) 403, 472
XmaJI CCTAGG 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 4 cut(s) 354, 435, 531, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.