Rh4DG034700

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005012
Physical Location & Seq
Reverse (-)
5874036 .. 5879871
5836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG034700.1

Sequence Viewer

Length: 258 bp
ATGCCACTGTCTTATTGTATAGCAGAGGGAAAGCCCTGCGTGCGTTGGGTTGAGAAGTACTTCAAGGACTGCCTCTGCAACCTCCGCGACAACTTCTTCTTCAACTTCTCCTTCAGTTTCGGGCTTGTCAGCTTAGTATGTTGGGGAGTTGCAGAAATCCCTAAGATAATCACCAACTTTCAGACTAAGTTGAGCCATGGAATCTCTCTTGCTTTCCTCCTCCTTGGGTTGCCGGTTGTATGCTGTAGGGGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

85

Amino Acids

9.62

Weight (kDa)

8.47

Isoelectric Point (pI)

15.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 36 - 79 6e-08 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 87
AciI CCGC 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 97
AfaI GTAC 1 cut(s) 59
AgsI TTSAA 2 cut(s) 64, 103
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 163
BfmI CTRYAG 1 cut(s) 244
BmcAI AGTACT 1 cut(s) 59
BmsI GCATC 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 196, 223
Bse118I RCCGGY 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 196, 223
BseGI GGATG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 233
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 1 cut(s) 85
BspFNI CGCG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 2 cut(s) 196, 223
BssT1I CCWWGG 2 cut(s) 196, 223
Bst4CI ACNGT 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 133, 162, 186
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 257
BstFNI CGCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 40, 84
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 257
BtsIMutI CAGTG 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 232
Csp6I GTAC 1 cut(s) 58
CviAII CATG 1 cut(s) 197
CviJI RGCY 4 cut(s) 34, 124, 132, 195
CviKI_1 RGCY 4 cut(s) 34, 124, 132, 195
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 3 cut(s) 133, 162, 186
Eco130I CCWWGG 2 cut(s) 196, 223
Eco57I CTGAAG 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 196, 223
ErhI CCWWGG 2 cut(s) 196, 223
FaeI CATG 1 cut(s) 200
FaiI YATR 4 cut(s) 20, 139, 198, 241
FatI CATG 1 cut(s) 196
HapII CCGG 1 cut(s) 233
Hin1II CATG 1 cut(s) 200
HinfI GANTC 1 cut(s) 201
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 163
Hpy188I TCNGA 1 cut(s) 183
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 78, 152
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 84
HpyF3I CTNAG 3 cut(s) 133, 162, 186
Hsp92II CATG 1 cut(s) 200
LpnPI CCDG 2 cut(s) 49, 246
LweI GCATC 1 cut(s) 242
MboII GAAGA 2 cut(s) 88, 91
MnlI CCTC 5 cut(s) 19, 83, 92, 227, 230
MroXI GAANNNNTTC 1 cut(s) 59
MspI CCGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 40, 84
NcoI CCATGG 1 cut(s) 196
NlaIII CATG 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 201
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
ScaI AGTACT 1 cut(s) 59
SetI ASST 2 cut(s) 84, 134
SfaNI GCATC 1 cut(s) 242
SfcI CTRYAG 1 cut(s) 244
SsiI CCGC 1 cut(s) 85
StyI CCWWGG 2 cut(s) 196, 223
TaaI ACNGT 1 cut(s) 9
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 1 cut(s) 12
TspRI CASTG 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.