Rorug02G0109600

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005012
Physical Location & Seq
Forward (+)
9430514 .. 9436253
5740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0109600.1

Sequence Viewer

Length: 4704 bp
ATGGGATCCGCCGCGGCCGCAGAAGCCGCCGCTGTTTCGAAGACCACCGCACCCAAAAACGAGCCCAAATCGACCTCTTCTTCGTCTTCCTCAGGAATGACAAAAAAGAAGGGTAAAAAAGCTTCCCAAAAGGAAGCGCCTACCAGTGGTAGAGGTAAGAAGCGAAATTCGCCTCCGAGCGGTGAAGAGCAATCCCAGTCCCGCAAAATGCCCAAGCGTGCTGCTGCATGTAAAGACTTTAAGGAGAGGTCTGTTCGTCTTTCTGAAAAGTCTGATTTCATCGCAAGCAAGGAGGAACAGATTGTGTCAGATGAAAGTCTTGCCGTAGGCTTGACTTGTGGACAGGATCCAGATGATGACAAGCCAAACAGAAGACTGACTCAGTTTATCTTGCATAATGAAGATGGTATTGCACAGCCACTTGAGATGGTGCAACATGGTGATCTGTTTATTACTGGCACTCTATTGCCTTTTAATGAAAGTTCTGACAAGGGCAAAGTCAAGGACAGGGGTATCAAATGTGAAGGTTTTGGCCGAATAGAAGCATGGGACATCTCTGGTTATGATGAGGGATATCCAATAATATGGCTTACAACTGATGTTGCTGATTATTGGTGTGTTAAACCTGCAAGCAGCTACAAGAAACACTATGATAATTTCTGTGAGAAGGCGCGTGCTTGCATAGAGGTCTACAAAGTACTGTCAAAATGCAACTCTGACTGCAGTCTTGATGAATTACTTGCTGGCATCGCTCGATCAATGAGTGGAAACAAGTTCTATGATGGCAGTGCTTCTATCAAAGAATTTATCCTATCTCAAGGTGTGTTTATCTATAATCAATTGGAAGGTTTGGAGGAGTCATCAACTAGGAATGATCGAATATTGGCACAGCTGCCTGTTCTCGCTGCCCTCAGAGAGGAGTGTATAAAGCATGGATATTATGTGCCCTCAAAACCAGCAGCTTCAAGTGGTACATTAAAGATTGCTTCAGATATCGGAGATGGACGGAATGGTTCATCCATTGTTGAAGCTGAGGAAGATGAAGATGCCAAGTTGGCTAGACTATTTCAAGAAGAAGAATACTTCAAGTCTATGAAACAAAGAAAACGTCAGGTTGCTGCCTCCACGTCCAAGAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTTTACCTGCCTATTATAAGACTGCTATTGAAGAGACAGATGAATTTATAGTGGTCGACGGTGAGGATGGTGATCTTCCCACACACATGCTTCACAACTGGTGTCTATACAATTCAGACTCGAGACTGATTTCACTGGAGCTTCTTCCAATGAAGGCTTGTGCTGATATTGATGTTACAATTTTTGGTTCTGGGGTTATGAGTGAAGATGATGGAAGCTGTGGGTTTGATCTCGATGCTGACAGTATCCAGTCTTCTTCAAGTGGTTCAATAGCACCAGTAGCACAGGATGCTTATGGCATGCCAGTTTACTTGAGTTCCATAAAGGAATGGGTGATAGAATGGGGAGCTTCAATGATTTCAGTATCAATTCGAACAGAACTGGCCTGGTACAGACTTGGCAAGCCATCAAAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACAATGCTTAAGGCAGAAAGTCGGGTAGCACGGCTCTCTTTTGCAGATGTCATTAAAAGATTGTCAGAGTTACCTAAGAATGATGGTGGTTACATTTCTTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGTACATGGACCTATAATATTTCAGCTGTTTACAGAATTTCCAGATGTGAAGATTAAAAATTGTCCATTTGTTCATGGTCTTCGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGTAGTAAATAAGAAGATTTTACAGAAGAGTGAATCAAATTTGAACCCAAGGGCATCGATGGGACCTGTGGTATCCAAGAGGAAGACGATGCAGGCTACAACAACGAAGCTGATTAACAGGATCTGGGGGGACTACTATTCAAATTACTCTCCTGAAGATTCAAGCCCGGGAGCAACTTGTGAAAAAATAGAAGAGGAAGAAGTTGAAGAAGAGGCCCAAGAGGACATAGAAGAGGATGATGCAGAAGAGAATTCATCAGTAATGGAAAAAGCCCAAAGGCCTTCTCCAATTTTAAGAAAAATTAAACCATGCTCCAGCAGCAAGAAGATTTTGTGGGATGAGGAGCCTTTGGGAAAAACATGTTCTGGTGAAGCTCTCTACAAGCATGCTGTTCTTCGTGGAGAAGAGATTTCTGTGGGTGGGGCTGTTTTGGTGGAAGTTGATGGTTCAGATGAAATTCCTGTTATCTATTTTGTAGAATACATGTATGAATCATCAGATGGAGAAAAAATGTTCCATGGCAGAATGATGCAGCGAGGATCTGATACTGTTCTTGGAAACACTGCTAATGAGAGGGAGCTATTCTTGACAAATGAGTGCATAAACTTGGCATTAAAGGATGTGAAACAGACAGTTGTTGTGGGTGTCAAGTCAATGCCATGGGGGCATCAATATAGGAAGGAGAATGCTGATGCTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAAGAAAGGATTGCCGACTGAATATTACTGTAGAAGCATGTATTGCCCTGAGCAAGGTGCTTTCTTTAGTCTTTCTCGTCATACTATTGGTCTGGGCTCTGGCTTCTGCCACTCTTGCAAAGTCAATGAAGATGAGAAGGCCAAAGAAGTCTTTGAAGTGAATTCATCAAAGACTGGTTTTATATACAGTGGAGATGAGTACTCAGTTCATGATTATGTCTACATAAGCCCCTACCAGTTTTCTGTGCAAACGATTGAAACTGAACTTTTCAAGGCTGGACGGAATTTGGGGTTGAAACCTTTTGTTGTGTGCCAAGTGCTGGGGATAATTACTGCAAAGGGATCTAAACAATCTGAAATGAAGTCTACCCAGGTTAAAGTTCGAAGATTTTTCAGACCAGAGGACATTTCGGTTGAGAAAGCATACTGCTCTGATATTAGAGAGGTCTACTACAGTGAAGAACTACATATTTTGCCTGTTGATAGCATAGAAGGAAAATGTGAAGTCAGAAAGAGGAGTGATCTTCCAGAATGCAATGCTCCTGCATTGTTTCAGCATATATTCTTCTGTGAGCATCTATATGATCCTTCTAACGGGTCTATTAAGCAGTTGCTGGCTAACATCAGGGTAAAGTACTCAACAGTAGGTGGTGATCCTGAATCTAGAAAGAGAAAGGGCAAGTGTAAAGAAGGAGAAGATGTTCCAGAAGTTCAGAAACAGAGGGATGATTCTGACCACAAACGCTTAGCCACTTTGGACATATTTGCTGGATGTGGTGGCTTATCGGAGGGGTTGCATCAGGCTGGTGTCTCAGTAACCAAGTGGGCAATCGAGTATGAAGAGCCAGCTGGACAGGCTTTCCAACTCAACCATCCTGAGTCCAAGGTGTTCATCAATAATTGCAATGTCATCTTGAAGGCTGTCATGGACAAATGTGGAGATGTGGATGATTGCATCTCAACCAGTGATGCCAATGATTTAGCTGCATCACTAGACGATAAGGTGAAAAATGATCTGCCCTTGCCAGGGCAAGTAGACTTTATTAATGGAGGCCCTCCATGCCAGGGTTTTTCTGGTATGAATAGATTCAATACCAGCACGTGGAGTAAAGTCCAGTGTGAAATGATATTGGCTTTCTTATCCTTTGCTGATTACTTTCGGCCAAAATACTTCCTCCTGGAGAATGTGAGGAACTTTGTGTCTTTTAATAAAGGACAGACATTCCGTCTGACTCTGGCTTCGCTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTATTCTGGAAGCTGGAGCTTACGGGGTTTCCCAGTCCCGAAAGCGAGCATTTATTTGGGCAGCTGCACCAGATGAGATTCTTCCCGAATGGCCAGAGCCAATGCATGTTTTTGGTGTGCCAGAGTTGAAGATCAATTTATCCAGTAATTCATATTATGCTGCTGTTCGGAGTACTGCAAGCGGAGCCCCTTTCCGACCCATAACGGTAAGAGACACAATCGGCGATCTCCCAGCTGTAGGCAATGGAGCATCCAAGGTCAATCTGGAGTATGAAAGTGACCCTGTCTCATGGTTCCAGAAGAAAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCAAAAGAAATGAACGAGCTGAACCTCATTCGGTGCAAGAGGATTCCCAAGAGGCCTGGTGCAGACTGGAAGGACCTTCCAGAGGAAAAAGTGACGCTCTCTACTGGTCAAGTAGTCGACTTGATACCATGGTGCCTGCCCAACACCGCCAAACGCCACAACCAGTGGAAGGGCCTGTTCGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCGTCACTGATCCTCAGCCAATGGGCAAGGTGGGAATGTGCTTCCATCCCGACCAGGACAGGATTATAACAGTTAGAGAATGCGCTCGTTCTCAAGGCTTTCCCGATAGCTACCAGTTTTATGGCAACACTCTCCACAAGCACAGGCAGATTGGAAACGCTGTCCCTCCAACTCTGGCTTATGCTCTCGGCCGAAAACTCAAGGAAGCAGTCGACAGCAAGAGCTCTTCTTCACAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

1567

Amino Acids

175.06

Weight (kDa)

5.9

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 119 - 252 2.6e-33 Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD PF12047 418 - 574 7.6e-34 Cytosine specific DNA methyltransferase replication foci domain
BAH PF01426 770 - 902 3.6e-20 BAH domain
BAH PF01426 944 - 1081 7.9e-24 BAH domain
DNA_methylase PF00145 1128 - 1555 4.4e-49 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1197, 4529
Acc36I ACCTGC 2 cut(s) 634, 1195
AccB1I GGYRCC 2 cut(s) 1887, 4380
AccB7I CCANNNNNTGG 2 cut(s) 2953, 3735
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 8 cut(s) 690, 1236, 2853, 2999, 3081, 3669, 4365, 4674
AccII CGCG 2 cut(s) 14, 673
AcoI YGGCCR 5 cut(s) 15, 532, 3794, 3998, 4651
AcuI CTGAAG 2 cut(s) 972, 2076
AcvI CACGTG 1 cut(s) 3735
AfaI GTAC 7 cut(s) 699, 973, 1571, 1788, 2834, 3269, 4081
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 2 cut(s) 2261, 2385
AhdI GACNNNNNGTC 1 cut(s) 3858
AjiI CACGTC 1 cut(s) 1128
AjnI CCWGG 7 cut(s) 1565, 3003, 3658, 3696, 3810, 4303, 4515
AloI GAACNNNNNNTCC 2 cut(s) 3318, 3350
Alw21I GWGCWC 1 cut(s) 4688
Alw26I GTCTC 6 cut(s) 1208, 1297, 1863, 3448, 4113, 4198
AlwNI CAGNNNCTG 3 cut(s) 1766, 2025, 3247
Ama87I CYCGRG 2 cut(s) 1300, 2068
ArsI GACNNNNNNTTYG 6 cut(s) 64, 96, 1093, 1125, 3856, 3888
AseI ATTAAT 2 cut(s) 1152, 3678
Asp700I GAANNNNTTC 2 cut(s) 3333, 3719
AspLEI GCGC 3 cut(s) 139, 673, 4547
AspS9I GGNCC 6 cut(s) 1793, 1964, 2116, 3686, 4321, 4420
AsuC2I CCSGG 2 cut(s) 2069, 2070
AsuHPI GGTGA 9 cut(s) 194, 452, 1253, 1262, 1525, 2282, 3296, 3649, 3910
AsuII TTCGAA 3 cut(s) 38, 1552, 3016
AvaI CYCGRG 2 cut(s) 1300, 2068
AvaII GGWCC 3 cut(s) 1793, 1964, 4321
AxyI CCTNAGG 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 948
BaeI ACNNNNGTAYC 4 cut(s) 496, 529, 4364, 4397
BalI TGGCCA 1 cut(s) 4000
BamHI GGATCC 2 cut(s) 5, 346
BanI GGYRCC 2 cut(s) 1887, 4380
BanII GRGCYC 4 cut(s) 66, 2732, 4096, 4688
BarI GAAGNNNNNNTAC 4 cut(s) 106, 138, 1417, 1449
BbrPI CACGTG 1 cut(s) 3735
BbsI GAAGAC 6 cut(s) 47, 78, 379, 1425, 1856, 1991
Bbv12I GWGCWC 1 cut(s) 4688
BbvCI CCTCAGC 2 cut(s) 1032, 4476
BceAI ACGGC 2 cut(s) 308, 1700
BciT130I CCWGG 7 cut(s) 1567, 3005, 3660, 3698, 3812, 4305, 4517
BciVI GTATCC 2 cut(s) 1436, 1984
BclI TGATCA 1 cut(s) 4240
BcnI CCSGG 2 cut(s) 2069, 2070
BcoDI GTCTC 6 cut(s) 1208, 1297, 1863, 3448, 4113, 4198
BfaI CTAG 4 cut(s) 867, 1059, 3297, 3626
BfmI CTRYAG 4 cut(s) 721, 2662, 3085, 4143
BfoI RGCGCY 1 cut(s) 140
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 2 cut(s) 634, 1195
BfuI GTATCC 2 cut(s) 1436, 1984
BglI GCCNNNNNGGC 2 cut(s) 1055, 2566
BlpI GCTNAGC 1 cut(s) 3379
BmcAI AGTACT 4 cut(s) 699, 2834, 3269, 4081
Bme1390I CCNGG 9 cut(s) 1567, 2069, 2070, 3005, 3660, 3698, 3812, 4305, 4517
Bme18I GGWCC 3 cut(s) 1793, 1964, 4321
BmeRI GACNNNNNGTC 1 cut(s) 3858
BmeT110I CYCGRG 2 cut(s) 1300, 2068
BmgBI CACGTC 1 cut(s) 1128
BmgT120I GGNCC 6 cut(s) 1793, 1964, 2116, 3686, 4321, 4420
BmiI GGNNCC 8 cut(s) 7, 348, 1889, 1965, 2247, 4093, 4202, 4382
BmrFI CCNGG 9 cut(s) 1567, 2069, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BmrI ACTGGG 3 cut(s) 190, 3934, 4450
BmuI ACTGGG 3 cut(s) 190, 3934, 4450
BoxI GACNNNNGTC 1 cut(s) 723
BpiI GAAGAC 6 cut(s) 47, 78, 379, 1425, 1856, 1991
BpmI CTGGAG 5 cut(s) 1337, 2200, 3833, 3942, 4193
Bpu10I CCTNAGC 3 cut(s) 1032, 2682, 4476
Bpu1102I GCTNAGC 1 cut(s) 3379
Bpu14I TTCGAA 3 cut(s) 38, 1552, 3016
BpuEI CTTGAG 6 cut(s) 443, 801, 1513, 3902, 4539, 4646
BpuMI CCSGG 2 cut(s) 2069, 2070
Bsa29I ATCGAT 1 cut(s) 1958
BsaAI YACGTR 1 cut(s) 3735
BsaBI GATNNNNATC 1 cut(s) 1251
BsaXI ACNNNNNCTCC 2 cut(s) 3318, 3348
Bse21I CCTNAGG 1 cut(s) 91
Bse3DI GCAATG 3 cut(s) 3175, 3544, 4156
Bse8I GATNNNNATC 1 cut(s) 1251
BseBI CCWGG 7 cut(s) 1567, 3005, 3660, 3698, 3812, 4305, 4517
BseCI ATCGAT 1 cut(s) 1958
BseJI GATNNNNATC 1 cut(s) 1251
BseMI GCAATG 3 cut(s) 3175, 3544, 4156
BseMII CTCAG 9 cut(s) 105, 395, 925, 1023, 2673, 2850, 3459, 3501, 4490
BseRI GAGGAG 4 cut(s) 869, 932, 2258, 3163
BseSI GKGCMC 1 cut(s) 948
BseX3I CGGCCG 2 cut(s) 15, 4651
BseYI CCCAGC 2 cut(s) 2953, 4138
BsgI GTGCAG 2 cut(s) 3957, 4329
Bsh1236I CGCG 2 cut(s) 14, 673
Bsh1285I CGRYCG 2 cut(s) 18, 4654
BshNI GGYRCC 2 cut(s) 1887, 4380
BshVI ATCGAT 1 cut(s) 1958
BsiEI CGRYCG 2 cut(s) 18, 4654
BsiHKAI GWGCWC 1 cut(s) 4688
BsiHKCI CYCGRG 2 cut(s) 1300, 2068
BsiSI CCGG 1 cut(s) 2069
BslFI GGGAC 6 cut(s) 184, 563, 1977, 2045, 3928, 4610
BsmAI GTCTC 6 cut(s) 1208, 1297, 1863, 3448, 4113, 4198
BsmFI GGGAC 6 cut(s) 184, 563, 1977, 2045, 3928, 4610
BsmI GAATGC 3 cut(s) 2593, 3170, 4547
BsoBI CYCGRG 2 cut(s) 1300, 2068
Bsp119I TTCGAA 3 cut(s) 38, 1552, 3016
Bsp1286I GDGCHC 5 cut(s) 66, 948, 2732, 4096, 4688
Bsp1407I TGTACA 1 cut(s) 1786
Bsp1720I GCTNAGC 1 cut(s) 3379
Bsp19I CCATGG 3 cut(s) 2419, 2561, 4376
BspCNI CTCAG 9 cut(s) 104, 394, 924, 1024, 2674, 2849, 3458, 3502, 4489
BspDI ATCGAT 1 cut(s) 1958
BspFNI CGCG 2 cut(s) 14, 673
BspHI TCATGA 1 cut(s) 2842
BspLI GGNNCC 8 cut(s) 7, 348, 1889, 1965, 2247, 4093, 4202, 4382
BspMAI CTGCAG 1 cut(s) 725
BspMI ACCTGC 2 cut(s) 634, 1195
BspQI GCTCTTC 3 cut(s) 180, 3468, 4693
BspT104I TTCGAA 3 cut(s) 38, 1552, 3016
BspT107I GGYRCC 2 cut(s) 1887, 4380
BspTI CTTAAG 1 cut(s) 1661
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 3 cut(s) 3175, 3544, 4156
BsrGI TGTACA 1 cut(s) 1786
BssT1I CCWWGG 6 cut(s) 1949, 2419, 2561, 3516, 4161, 4376
Bst2UI CCWGG 7 cut(s) 1567, 3005, 3660, 3698, 3812, 4305, 4517
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 2 cut(s) 1469, 2676
BstAUI TGTACA 1 cut(s) 1786
BstBAI YACGTR 1 cut(s) 3735
BstBI TTCGAA 3 cut(s) 38, 1552, 3016
BstDSI CCRYGG 4 cut(s) 12, 2419, 2561, 4376
BstENI CCTNNNNNAGG 3 cut(s) 914, 2685, 4328
BstFNI CGCG 2 cut(s) 14, 673
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 3 cut(s) 139, 673, 4547
BstMAI GTCTC 6 cut(s) 1208, 1297, 1863, 3448, 4113, 4198
BstMCI CGRYCG 2 cut(s) 18, 4654
BstNI CCWGG 7 cut(s) 1567, 3005, 3660, 3698, 3812, 4305, 4517
BstNSI RCATGY 8 cut(s) 231, 1270, 1483, 2265, 2291, 2389, 2674, 4016
BstPAI GACNNNNGTC 1 cut(s) 723
BstSCI CCNGG 9 cut(s) 1565, 2067, 2068, 3003, 3658, 3696, 3810, 4303, 4515
BstSFI CTRYAG 4 cut(s) 721, 2662, 3085, 4143
BstSLI GKGCMC 1 cut(s) 948
BstUI CGCG 2 cut(s) 14, 673
BstV2I GAAGAC 6 cut(s) 47, 78, 379, 1425, 1856, 1991
BstX2I RGATCY 5 cut(s) 5, 346, 2022, 2441, 2975
BstXI CCANNNNNNTGG 2 cut(s) 585, 4583
BstYI RGATCY 5 cut(s) 5, 346, 2022, 2441, 2975
BstZI CGGCCG 2 cut(s) 15, 4651
Bsu15I ATCGAT 1 cut(s) 1958
Bsu36I CCTNAGG 1 cut(s) 91
BsuI GTATCC 2 cut(s) 1436, 1984
BsuTUI ATCGAT 1 cut(s) 1958
BtgI CCRYGG 4 cut(s) 12, 2419, 2561, 4376
BtgZI GCGATG 2 cut(s) 265, 733
BtrI CACGTC 1 cut(s) 1128
BtsI GCAGTG 2 cut(s) 793, 2463
BveI ACCTGC 2 cut(s) 634, 1195
CaiI CAGNNNCTG 3 cut(s) 1766, 2025, 3247
CciI TCATGA 1 cut(s) 2842
CciNI GCGGCCGC 1 cut(s) 15
CfoI GCGC 3 cut(s) 139, 673, 4547
Cfr13I GGNCC 6 cut(s) 1793, 1964, 2116, 3686, 4321, 4420
Cfr42I CCGCGG 1 cut(s) 15
Cfr9I CCCGGG 1 cut(s) 2068
ClaI ATCGAT 1 cut(s) 1958
CseI GACGC 1 cut(s) 4351
Csp6I GTAC 7 cut(s) 698, 972, 1570, 1787, 2833, 3268, 4080
CviQI GTAC 7 cut(s) 698, 972, 1570, 1787, 2833, 3268, 4080
DriI GACNNNNNGTC 1 cut(s) 3858
EaeI YGGCCR 5 cut(s) 15, 532, 3794, 3998, 4651
EagI CGGCCG 2 cut(s) 15, 4651
Eam1105I GACNNNNNGTC 1 cut(s) 3858
Ecl136II GAGCTC 1 cut(s) 4686
EclXI CGGCCG 2 cut(s) 15, 4651
Eco130I CCWWGG 6 cut(s) 1949, 2419, 2561, 3516, 4161, 4376
Eco147I AGGCCT 2 cut(s) 2182, 4303
Eco24I GRGCYC 4 cut(s) 66, 2732, 4096, 4688
Eco32I GATATC 2 cut(s) 575, 994
Eco47I GGWCC 3 cut(s) 1793, 1964, 4321
Eco52I CGGCCG 2 cut(s) 15, 4651
Eco53kI GAGCTC 1 cut(s) 4686
Eco57I CTGAAG 2 cut(s) 972, 2076
Eco72I CACGTG 1 cut(s) 3735
Eco81I CCTNAGG 1 cut(s) 91
Eco88I CYCGRG 2 cut(s) 1300, 2068
EcoICRI GAGCTC 1 cut(s) 4686
EcoNI CCTNNNNNAGG 3 cut(s) 914, 2685, 4328
EcoO109I RGGNCCY 4 cut(s) 1964, 3686, 4321, 4420
EcoRI GAATTC 2 cut(s) 2152, 2794
EcoRII CCWGG 7 cut(s) 1565, 3003, 3658, 3696, 3810, 4303, 4515
EcoRV GATATC 2 cut(s) 575, 994
EcoT14I CCWWGG 6 cut(s) 1949, 2419, 2561, 3516, 4161, 4376
EcoT22I ATGCAT 1 cut(s) 4014
EcoT38I GRGCYC 4 cut(s) 66, 2732, 4096, 4688
ErhI CCWWGG 6 cut(s) 1949, 2419, 2561, 3516, 4161, 4376
FaqI GGGAC 6 cut(s) 184, 563, 1977, 2045, 3928, 4610
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 4240
FblI GTMKAC 8 cut(s) 690, 1236, 2853, 2999, 3081, 3669, 4365, 4674
FriOI GRGCYC 4 cut(s) 66, 2732, 4096, 4688
FspBI CTAG 4 cut(s) 867, 1059, 3297, 3626
GlaI GCGC 3 cut(s) 138, 672, 4546
GsaI CCCAGC 2 cut(s) 2957, 4142
GsuI CTGGAG 5 cut(s) 1337, 2200, 3833, 3942, 4193
HaeII RGCGCY 1 cut(s) 140
HapII CCGG 1 cut(s) 2069
HgaI GACGC 1 cut(s) 4351
HhaI GCGC 3 cut(s) 139, 673, 4547
Hin6I GCGC 3 cut(s) 137, 671, 4545
HinP1I GCGC 3 cut(s) 137, 671, 4545
HincII GTYRAC 3 cut(s) 1237, 4366, 4675
HindII GTYRAC 3 cut(s) 1237, 4366, 4675
HindIII AAGCTT 1 cut(s) 120
HpaII CCGG 1 cut(s) 2069
HphI GGTGA 9 cut(s) 194, 452, 1253, 1262, 1525, 2282, 3296, 3649, 3910
Hpy99I CGWCG 1 cut(s) 1241
HpyCH4IV ACGT 3 cut(s) 1108, 1127, 3734
HpySE526I ACGT 3 cut(s) 1108, 1127, 3734
HspAI GCGC 3 cut(s) 137, 671, 4545
Ksp22I TGATCA 1 cut(s) 4240
KspI CCGCGG 1 cut(s) 15
LguI GCTCTTC 3 cut(s) 180, 3468, 4693
MaeI CTAG 4 cut(s) 867, 1059, 3297, 3626
MaeII ACGT 3 cut(s) 1108, 1127, 3734
MaeIII GTNAC 7 cut(s) 1354, 1722, 1742, 3448, 4184, 4339, 4465
MbiI CCGCTC 1 cut(s) 180
MfeI CAATTG 1 cut(s) 839
MflI RGATCY 5 cut(s) 5, 346, 2022, 2441, 2975
MhlI GDGCHC 5 cut(s) 66, 948, 2732, 4096, 4688
MlsI TGGCCA 1 cut(s) 4000
MluNI TGGCCA 1 cut(s) 4000
MlyI GAGTC 5 cut(s) 373, 866, 1292, 3521, 3859
MmeI TCCRAC 4 cut(s) 3520, 4126, 4416, 4655
Mox20I TGGCCA 1 cut(s) 4000
Mph1103I ATGCAT 1 cut(s) 4014
MroXI GAANNNNTTC 2 cut(s) 3333, 3719
MscI TGGCCA 1 cut(s) 4000
MslI CAYNNNNRTG 3 cut(s) 1265, 2847, 3213
Msp20I TGGCCA 1 cut(s) 4000
MspA1I CMGCKG 7 cut(s) 14, 32, 892, 1810, 3482, 3971, 4142
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 1 cut(s) 2069
MspR9I CCNGG 9 cut(s) 1567, 2069, 2070, 3005, 3660, 3698, 3812, 4305, 4517
MunI CAATTG 1 cut(s) 839
Mva1269I GAATGC 3 cut(s) 2593, 3170, 4547
MvaI CCWGG 7 cut(s) 1567, 3005, 3660, 3698, 3812, 4305, 4517
MvnI CGCG 2 cut(s) 14, 673
NciI CCSGG 2 cut(s) 2069, 2070
NcoI CCATGG 3 cut(s) 2419, 2561, 4376
NlaIV GGNNCC 8 cut(s) 7, 348, 1889, 1965, 2247, 4093, 4202, 4382
NmeAIII GCCGAG 1 cut(s) 4629
NmuCI GTSAC 3 cut(s) 4184, 4339, 4465
NotI GCGGCCGC 1 cut(s) 15
NsiI ATGCAT 1 cut(s) 4014
NspI RCATGY 8 cut(s) 231, 1270, 1483, 2265, 2291, 2389, 2674, 4016
NspV TTCGAA 3 cut(s) 38, 1552, 3016
PaeI GCATGC 2 cut(s) 1483, 2291
PaeR7I CTCGAG 1 cut(s) 1300
PagI TCATGA 1 cut(s) 2842
PceI AGGCCT 2 cut(s) 2182, 4303
PciI ACATGT 2 cut(s) 2261, 2385
PciSI GCTCTTC 3 cut(s) 180, 3468, 4693
PctI GAATGC 3 cut(s) 2593, 3170, 4547
PdmI GAANNNNTTC 2 cut(s) 3333, 3719
PfeI GAWTC 8 cut(s) 1934, 2060, 2393, 3293, 3362, 3720, 3985, 4291
PflFI GACNNNGTC 1 cut(s) 4190
PflMI CCANNNNNTGG 2 cut(s) 2953, 3735
PfoI TCCNGGA 1 cut(s) 3810
PleI GAGTC 5 cut(s) 373, 865, 1292, 3520, 3859
PmaCI CACGTG 1 cut(s) 3735
PmlI CACGTG 1 cut(s) 3735
PpsI GAGTC 5 cut(s) 373, 865, 1292, 3520, 3859
Ppu21I YACGTR 1 cut(s) 3735
PpuMI RGGWCCY 2 cut(s) 1964, 4321
PscI ACATGT 2 cut(s) 2261, 2385
PshAI GACNNNNGTC 1 cut(s) 723
PshBI ATTAAT 2 cut(s) 1152, 3678
PsiI TTATAA 2 cut(s) 1197, 4529
Psp124BI GAGCTC 1 cut(s) 4688
Psp5II RGGWCCY 2 cut(s) 1964, 4321
Psp6I CCWGG 7 cut(s) 1565, 3003, 3658, 3696, 3810, 4303, 4515
PspCI CACGTG 1 cut(s) 3735
PspFI CCCAGC 2 cut(s) 2953, 4138
PspGI CCWGG 7 cut(s) 1565, 3003, 3658, 3696, 3810, 4303, 4515
PspN4I GGNNCC 8 cut(s) 7, 348, 1889, 1965, 2247, 4093, 4202, 4382
PspPI GGNCC 6 cut(s) 1793, 1964, 2116, 3686, 4321, 4420
PspPPI RGGWCCY 2 cut(s) 1964, 4321
PstI CTGCAG 1 cut(s) 725
PstNI CAGNNNCTG 3 cut(s) 1766, 2025, 3247
PsuI RGATCY 5 cut(s) 5, 346, 2022, 2441, 2975
PsyI GACNNNGTC 1 cut(s) 4190
PvuII CAGCTG 5 cut(s) 892, 1810, 3482, 3971, 4142
RsaI GTAC 7 cut(s) 699, 973, 1571, 1788, 2834, 3269, 4081
RsaNI GTAC 7 cut(s) 698, 972, 1570, 1787, 2833, 3268, 4080
RseI CAYNNNNRTG 3 cut(s) 1265, 2847, 3213
SacI GAGCTC 1 cut(s) 4688
SacII CCGCGG 1 cut(s) 15
SalI GTCGAC 3 cut(s) 1235, 4364, 4673
SapI GCTCTTC 3 cut(s) 180, 3468, 4693
Sau96I GGNCC 6 cut(s) 1793, 1964, 2116, 3686, 4321, 4420
ScaI AGTACT 4 cut(s) 699, 2834, 3269, 4081
SchI GAGTC 5 cut(s) 373, 866, 1292, 3521, 3859
ScrFI CCNGG 9 cut(s) 1567, 2069, 2070, 3005, 3660, 3698, 3812, 4305, 4517
SduI GDGCHC 5 cut(s) 66, 948, 2732, 4096, 4688
SfcI CTRYAG 4 cut(s) 721, 2662, 3085, 4143
Sfr274I CTCGAG 1 cut(s) 1300
Sfr303I CCGCGG 1 cut(s) 15
SfuI TTCGAA 3 cut(s) 38, 1552, 3016
SgrBI CCGCGG 1 cut(s) 15
SinI GGWCC 3 cut(s) 1793, 1964, 4321
SlaI CTCGAG 1 cut(s) 1300
SmaI CCCGGG 1 cut(s) 2070
SmiMI CAYNNNNRTG 3 cut(s) 1265, 2847, 3213
SmlI CTYRAG 8 cut(s) 422, 816, 1300, 1492, 1661, 3881, 4554, 4661
SmoI CTYRAG 8 cut(s) 422, 816, 1300, 1492, 1661, 3881, 4554, 4661
SphI GCATGC 2 cut(s) 1483, 2291
SseBI AGGCCT 2 cut(s) 2182, 4303
SspI AATATT 3 cut(s) 882, 1803, 2657
SspMI CTAG 4 cut(s) 867, 1059, 3297, 3626
SstI GAGCTC 1 cut(s) 4688
StuI AGGCCT 2 cut(s) 2182, 4303
StyD4I CCNGG 9 cut(s) 1565, 2067, 2068, 3003, 3658, 3696, 3810, 4303, 4515
StyI CCWWGG 6 cut(s) 1949, 2419, 2561, 3516, 4161, 4376
TaiI ACGT 3 cut(s) 1111, 1130, 3737
TatI WGTACW 5 cut(s) 697, 1786, 2832, 3267, 4079
TauI GCSGC 5 cut(s) 14, 17, 20, 29, 32
TfiI GAWTC 8 cut(s) 1934, 2060, 2393, 3293, 3362, 3720, 3985, 4291
TseFI GTSAC 3 cut(s) 4184, 4339, 4465
Tsp45I GTSAC 3 cut(s) 4184, 4339, 4465
TspGWI ACGGA 4 cut(s) 1021, 2929, 3848, 4453
TspMI CCCGGG 1 cut(s) 2068
Tth111I GACNNNGTC 1 cut(s) 4190
Van91I CCANNNNNTGG 2 cut(s) 2953, 3735
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 3 cut(s) 1793, 1964, 4321
VspI ATTAAT 2 cut(s) 1152, 3678
XagI CCTNNNNNAGG 3 cut(s) 914, 2685, 4328
XbaI TCTAGA 1 cut(s) 3296
XceI RCATGY 8 cut(s) 231, 1270, 1483, 2265, 2291, 2389, 2674, 4016
XcmI CCANNNNNNNNNTGG 2 cut(s) 3704, 4168
XhoI CTCGAG 1 cut(s) 1300
XmaI CCCGGG 1 cut(s) 2068
XmiI GTMKAC 8 cut(s) 690, 1236, 2853, 2999, 3081, 3669, 4365, 4674
XmnI GAANNNNTTC 2 cut(s) 3333, 3719
XspI CTAG 4 cut(s) 867, 1059, 3297, 3626
ZrmI AGTACT 4 cut(s) 699, 2834, 3269, 4081
Zsp2I ATGCAT 1 cut(s) 4014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.