Rh2BG164600

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005012
Physical Location & Seq
Reverse (-)
14476876 .. 14481603
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG164600.1

Sequence Viewer

Length: 939 bp
ATGTTCAAGTATGTCCAAGTGACTAGGAATGGAGATTTTGGTCATTACATTATGACACATAACACTATATTGTTTTTTTGTTTTGGACAGCTTTACACAACAAGCACTGTAGTGTTGGTGTTGCAGAGCGTGTACTATGATTACTTCTATACATGGTGGAAATGTGGCAATGTCACAGTTAACCAAGTGGCACAAGATGAAGAAGAAGATTCCAGAAAACCATTGAATCCAAAGTTGGTTGATTCAGGCGTCCCAATACCGAGTGCTTCACCCAAAGCCACTCCTAGAAGGGAGTTCTACTATACGTCAGCTAGATCGATGGCGGGTAGTGGCACTCCGCCGTTTCGAACGTATATGAAGGTAGCCAGAAGTGGTCCTTCTACATTGGCAGTAGACAGTGACTCATCTTCGGAGGACGAGTCGGCTCCAGTTACATCCAAAAAATCTGATAGGACTTCCCAACCCAGGCCAATTCCAAGATCGGTAGGTTATGGGGCATTTTTAGCTACATCACTTAACTTGCCCTTCGGAAGCAAGGGAATGACACAAGTATTATACATAGGAATTACTGGGAGGAAGCTATTGCAGGAACACTCGGTGGAGCATAGTGTTTATGGGCAATGGTTGGGGTGGCTGATGGCTGCTATATACATGGGCGGTCGACTACCTCAGATTTGGTTAAACATTAAAAGAGGGAGTGTGGAGGGCTTGAATCCTCTAATGTTCGTCTTTGCATTGGTTGCAAATGTTACTTATGTGGCCAGCATACTTGTAAGAACAATCGAGTGGGATAACATTAAAGCCAATATGCCATGGTTGCTCGATGCTGCAGTTTGCGTGGTGCTCGACTTATTTATAATACTGCAGTACATGTATTACAGATACTGGAGGAAGAGGACTACAAGTGGTGATGAGGAAGACAACATATACTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

312

Amino Acids

35.43

Weight (kDa)

8.91

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 206 - 261 7.7e-17 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
AccI GTMKAC 2 cut(s) 393, 661
AciI CCGC 3 cut(s) 323, 338, 657
AcoI YGGCCR 1 cut(s) 759
AcyI GRCGYC 1 cut(s) 249
AdeI CACNNNGTG 1 cut(s) 598
AfaI GTAC 2 cut(s) 134, 869
AfiI CCNNNNNNNGG 1 cut(s) 465
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 3 cut(s) 7, 226, 712
AjnI CCWGG 1 cut(s) 464
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 4 cut(s) 91, 311, 506, 580
AluI AGCT 4 cut(s) 91, 311, 506, 580
Alw21I GWGCWC 1 cut(s) 846
AlwNI CAGNNNCTG 1 cut(s) 885
AoxI GGCC 2 cut(s) 467, 759
ApeKI GCWGC 2 cut(s) 641, 827
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 261, 920
AsuII TTCGAA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 374
BalI TGGCCA 1 cut(s) 761
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 2 cut(s) 628, 814
BccI CCATC 2 cut(s) 313, 631
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 466
BfaI CTAG 3 cut(s) 24, 285, 312
BfmI CTRYAG 3 cut(s) 108, 828, 863
BisI GCNGC 2 cut(s) 642, 828
BlsI GCNGC 2 cut(s) 643, 829
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 1 cut(s) 466
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 1 cut(s) 814
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 2 cut(s) 411, 907
Bpu14I TTCGAA 1 cut(s) 346
Bsa29I ATCGAT 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 2 cut(s) 464, 812
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse1I ACTGG 3 cut(s) 428, 574, 890
Bse3DI GCAATG 2 cut(s) 175, 626
BseBI CCWGG 1 cut(s) 466
BseCI ATCGAT 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 464, 812
BseGI GGATG 1 cut(s) 434
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMI GCAATG 2 cut(s) 175, 626
BseMII CTCAG 1 cut(s) 683
BseNI ACTGG 3 cut(s) 428, 574, 890
BseXI GCAGC 2 cut(s) 628, 814
Bsh1285I CGRYCG 1 cut(s) 661
BshFI GGCC 2 cut(s) 469, 761
BshVI ATCGAT 1 cut(s) 317
BsiEI CGRYCG 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 846
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 465
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 2 cut(s) 469, 761
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 846
Bsp143I GATC 2 cut(s) 314, 479
Bsp19I CCATGG 1 cut(s) 812
BspACI CCGC 3 cut(s) 323, 338, 657
BspANI GGCC 2 cut(s) 469, 761
BspCNI CTCAG 1 cut(s) 682
BspDI ATCGAT 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 426
BspMAI CTGCAG 2 cut(s) 832, 867
BspT104I TTCGAA 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 175, 626
BsrI ACTGG 3 cut(s) 428, 574, 890
BssECI CCNNGG 2 cut(s) 464, 812
BssMI GATC 2 cut(s) 314, 479
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 812
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 3 cut(s) 109, 178, 398
Bst6I CTCTTC 1 cut(s) 887
BstACI GRCGYC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 1 cut(s) 669
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 1 cut(s) 434
BstKTI GATC 2 cut(s) 317, 482
BstMBI GATC 2 cut(s) 314, 479
BstMCI CGRYCG 1 cut(s) 661
BstMWI GCNNNNNNNGC 3 cut(s) 503, 740, 817
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 1 cut(s) 874
BstSCI CCNGG 1 cut(s) 464
BstSFI CTRYAG 3 cut(s) 108, 828, 863
BstV1I GCAGC 2 cut(s) 628, 814
BstV2I GAAGAC 1 cut(s) 924
Bsu15I ATCGAT 1 cut(s) 317
BsuRI GGCC 2 cut(s) 469, 761
BsuTUI ATCGAT 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 812
BtsCI GGATG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 105, 403
Cac8I GCNNGC 1 cut(s) 763
CaiI CAGNNNCTG 1 cut(s) 885
Cfr13I GGNCC 1 cut(s) 374
ClaI ATCGAT 1 cut(s) 317
CseI GACGC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 133, 868
CviAII CATG 4 cut(s) 153, 652, 813, 871
CviQI GTAC 2 cut(s) 133, 868
DdeI CTNAG 1 cut(s) 669
DpnI GATC 2 cut(s) 316, 481
DpnII GATC 2 cut(s) 314, 479
DraIII CACNNNGTG 1 cut(s) 598
EaeI YGGCCR 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
EciI GGCGGA 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 812
Eco47I GGWCC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 812
ErhI CCWWGG 1 cut(s) 812
FaeI CATG 4 cut(s) 156, 655, 816, 874
FalI AAGNNNNNCTT 2 cut(s) 361, 393
FaqI GGGAC 1 cut(s) 236
FatI CATG 4 cut(s) 152, 651, 812, 870
FauI CCCGC 1 cut(s) 316
FblI GTMKAC 2 cut(s) 393, 661
Fnu4HI GCNGC 2 cut(s) 642, 828
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 642, 828
FspBI CTAG 3 cut(s) 24, 285, 312
GluI GCNGC 2 cut(s) 642, 828
GsuI CTGGAG 2 cut(s) 411, 907
HaeIII GGCC 2 cut(s) 469, 761
HgaI GACGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 4 cut(s) 156, 655, 816, 874
HincII GTYRAC 2 cut(s) 181, 662
HindII GTYRAC 2 cut(s) 181, 662
HinfI GANTC 6 cut(s) 209, 226, 242, 401, 419, 712
HpaI GTTAAC 1 cut(s) 181
HphI GGTGA 2 cut(s) 261, 920
Hpy166II GTNNAC 4 cut(s) 133, 181, 394, 662
Hpy188I TCNGA 4 cut(s) 412, 448, 530, 672
Hpy188III TCNNGA 1 cut(s) 213
Hpy8I GTNNAC 4 cut(s) 133, 181, 394, 662
HpyAV CCTTC 4 cut(s) 282, 352, 387, 535
HpyCH4III ACNGT 3 cut(s) 109, 178, 398
HpyCH4IV ACGT 2 cut(s) 305, 350
HpyCH4V TGCA 6 cut(s) 124, 586, 734, 743, 830, 865
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 740, 817
HpyF3I CTNAG 1 cut(s) 669
HpySE526I ACGT 2 cut(s) 305, 350
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 4 cut(s) 156, 655, 816, 874
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 314, 479
LmnI GCTCC 2 cut(s) 430, 601
Lsp1109I GCAGC 2 cut(s) 628, 814
LweI GCATC 1 cut(s) 814
MaeI CTAG 3 cut(s) 24, 285, 312
MaeII ACGT 2 cut(s) 305, 350
MaeIII GTNAC 5 cut(s) 19, 172, 398, 430, 748
MalI GATC 2 cut(s) 316, 481
MboI GATC 2 cut(s) 314, 479
MboII GAAGA 6 cut(s) 212, 215, 218, 399, 904, 929
MhlI GDGCHC 1 cut(s) 846
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 2 cut(s) 471, 564
MluNI TGGCCA 1 cut(s) 761
MlyI GAGTC 2 cut(s) 395, 428
MnlI CCTC 9 cut(s) 406, 567, 678, 686, 697, 726, 882, 888, 907
Mox20I TGGCCA 1 cut(s) 761
MscI TGGCCA 1 cut(s) 761
MseI TTAA 5 cut(s) 180, 516, 680, 687, 798
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 761
MspR9I CCNGG 1 cut(s) 466
MvaI CCWGG 1 cut(s) 466
MwoI GCNNNNNNNGC 3 cut(s) 503, 740, 817
NcoI CCATGG 1 cut(s) 812
NdeII GATC 2 cut(s) 314, 479
NlaIII CATG 4 cut(s) 156, 655, 816, 874
NlaIV GGNNCC 1 cut(s) 426
NmuCI GTSAC 3 cut(s) 19, 172, 398
NspI RCATGY 1 cut(s) 874
NspV TTCGAA 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 110
PciI ACATGT 1 cut(s) 870
PfeI GAWTC 4 cut(s) 209, 226, 242, 712
PkrI GCNGC 2 cut(s) 643, 829
PleI GAGTC 2 cut(s) 395, 427
PpsI GAGTC 2 cut(s) 395, 427
PscI ACATGT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 464
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 1 cut(s) 374
PstI CTGCAG 2 cut(s) 832, 867
PstNI CAGNNNCTG 1 cut(s) 885
RsaI GTAC 2 cut(s) 134, 869
RsaNI GTAC 2 cut(s) 133, 868
RseI CAYNNNNRTG 1 cut(s) 110
SalI GTCGAC 1 cut(s) 660
SaqAI TTAA 5 cut(s) 180, 516, 680, 687, 798
SatI GCNGC 2 cut(s) 642, 828
Sau3AI GATC 2 cut(s) 314, 479
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 2 cut(s) 395, 428
ScrFI CCNGG 1 cut(s) 466
SduI GDGCHC 1 cut(s) 846
SetI ASST 9 cut(s) 93, 308, 313, 353, 363, 490, 508, 582, 670
SfaNI GCATC 1 cut(s) 814
SfcI CTRYAG 3 cut(s) 108, 828, 863
SfuI TTCGAA 1 cut(s) 346
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 110
Sse9I AATT 2 cut(s) 471, 564
SsiI CCGC 3 cut(s) 323, 338, 657
SspMI CTAG 3 cut(s) 24, 285, 312
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 1 cut(s) 812
TaaI ACNGT 3 cut(s) 109, 178, 398
TaiI ACGT 2 cut(s) 308, 353
TaqI TCGA 6 cut(s) 317, 346, 661, 783, 822, 846
TasI AATT 2 cut(s) 471, 564
TatI WGTACW 2 cut(s) 132, 867
TfiI GAWTC 4 cut(s) 209, 226, 242, 712
Tru1I TTAA 5 cut(s) 180, 516, 680, 687, 798
Tru9I TTAA 5 cut(s) 180, 516, 680, 687, 798
TscAI CASTG 2 cut(s) 112, 403
TseFI GTSAC 3 cut(s) 19, 172, 398
TseI GCWGC 2 cut(s) 641, 827
Tsp45I GTSAC 3 cut(s) 19, 172, 398
TspDTI ATGAA 2 cut(s) 213, 371
TspRI CASTG 2 cut(s) 112, 403
VpaK11BI GGWCC 1 cut(s) 374
XceI RCATGY 1 cut(s) 874
XmiI GTMKAC 2 cut(s) 393, 661
XspI CTAG 3 cut(s) 24, 285, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.