Rw2G012310

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005012
Physical Location & Seq
Reverse (-)
12754611 .. 12759121
4511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012310.1

Sequence Viewer

Length: 933 bp
ATGCCACTGTCTTATTGTATAGCAGAGAGAAAGCCCTGCGTGCGTTGGGTTGAGAAATACTTCAAGGACTGCCTCTGCAACCTCCGCGACGACTTCTCCTTCGGTTTCGGGCTTGTCAGCTTAGTCTGTTGGGGAGTTGCAGAAATCCCTCAGATAATCACCAACTTTCAGACTAAGTCAAGCCATGGAGTCTCTCTTGCTTTTCTCCTCACTTGGGTTGCCGGGGATGTGTTTAATCTGGTGGGTTGTCTTCTGGAACCAGCAACTTTGCCTACCCAGTACTACACAGCTCTGCTTTACACAACAAGCACTGTAGTGTTGGTGTTGCAGAGCGTGTACTATGATTACTTCTATACATGGTGGAAATGTGGCAAAGTCACAGTTAACCAAGTGGCACAAGATGAAGAAGAAGATTCCAGAAAACCATTGAATCCAAAGTTGGTTGATTCAGGCGTCCCAATACCGAGTGCTTCACCCAAAGCCACTCCTAGAAGGGAGTTCTACTATACGTCAGCTAGATCGATGGCGGGTAGTGGCACTCCGCCGTTTCGAACGTATATGAAGGTAGCCAGAAGTGGTCCTTCTACATTGGCAGTAGACAGTGACTCATCTTCGGAGGACGAGTCGGCTCCAGTTACATCCAAAAAATCTGATAGGACTTCCCAACCCAGGCCAATTCCAAGATCGGTAGGTTATGGGGCATTTTTAGCTACATCACTTAACTTGCCCTTGGGAAGCAAGGGAATGACACAAGTATTATACATAGGAATTACTGGGAGGAAGCTATTGCAGGAACACTCGGTGGAGCATAGTGTTTATGGGCAATGGTTGGGGTGGCTGATGGCTGCTATATACATGGGCGGTCGACTACCTCAGATTTGGTTAAACGTATGTATATACTTCAATGATCTAAAAGCTAGATTCCGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

310

Amino Acids

34.68

Weight (kDa)

8.68

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 32 - 88 3e-18 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 597, 865
AccII CGCG 1 cut(s) 87
AciI CCGC 4 cut(s) 85, 527, 542, 861
AcyI GRCGYC 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 802
AfaI GTAC 2 cut(s) 281, 338
AfiI CCNNNNNNNGG 2 cut(s) 214, 669
AgsI TTSAA 3 cut(s) 64, 430, 904
AjnI CCWGG 1 cut(s) 668
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 6 cut(s) 120, 290, 515, 710, 784, 917
AluI AGCT 6 cut(s) 120, 290, 515, 710, 784, 917
Alw26I GTCTC 1 cut(s) 196
AoxI GGCC 1 cut(s) 671
ApeKI GCWGC 1 cut(s) 845
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
Asp700I GAANNNNTTC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 578
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 151, 465
AsuII TTCGAA 1 cut(s) 550
AvaII GGWCC 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 832
BccI CCATC 2 cut(s) 517, 835
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 670
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 3 cut(s) 489, 516, 918
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 846
BlsI GCNGC 1 cut(s) 847
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 223, 670
Bme18I GGWCC 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 2 cut(s) 258, 630
BmrFI CCNGG 2 cut(s) 223, 670
BmrI ACTGGG 2 cut(s) 271, 783
BmuI ACTGGG 2 cut(s) 271, 783
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 615
Bpu14I TTCGAA 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 4 cut(s) 184, 222, 668, 729
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 608, 638
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 669
Bse1I ACTGG 3 cut(s) 277, 632, 778
Bse3DI GCAATG 1 cut(s) 830
BseBI CCWGG 1 cut(s) 670
BseCI ATCGAT 1 cut(s) 521
BseDI CCNNGG 4 cut(s) 184, 222, 668, 729
BseGI GGATG 2 cut(s) 232, 638
BseLI CCNNNNNNNGG 2 cut(s) 214, 669
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 2 cut(s) 164, 887
BseNI ACTGG 3 cut(s) 277, 632, 778
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 832
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 865
BshFI GGCC 1 cut(s) 673
BshVI ATCGAT 1 cut(s) 521
BsiEI CGRYCG 1 cut(s) 865
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 214, 669
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 1 cut(s) 673
Bsp119I TTCGAA 1 cut(s) 550
Bsp143I GATC 3 cut(s) 518, 683, 907
Bsp19I CCATGG 1 cut(s) 184
BspACI CCGC 4 cut(s) 85, 527, 542, 861
BspANI GGCC 1 cut(s) 673
BspCNI CTCAG 2 cut(s) 163, 886
BspDI ATCGAT 1 cut(s) 521
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 258, 630
BspT104I TTCGAA 1 cut(s) 550
BsrDI GCAATG 1 cut(s) 830
BsrI ACTGG 3 cut(s) 277, 632, 778
BssECI CCNNGG 4 cut(s) 184, 222, 668, 729
BssMI GATC 3 cut(s) 518, 683, 907
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 184, 729
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 4 cut(s) 9, 313, 382, 602
BstACI GRCGYC 1 cut(s) 453
BstBI TTCGAA 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 4 cut(s) 121, 150, 174, 873
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 232, 638
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 521, 686, 910
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 3 cut(s) 518, 683, 907
BstMCI CGRYCG 1 cut(s) 865
BstMWI GCNNNNNNNGC 3 cut(s) 40, 84, 707
BstNI CCWGG 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 221, 668
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 832
BstV2I GAAGAC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 521
BsuRI GGCC 1 cut(s) 673
BsuTUI ATCGAT 1 cut(s) 521
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 232, 638
BtsIMutI CAGTG 3 cut(s) 5, 309, 607
Cac8I GCNNGC 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 578
ClaI ATCGAT 1 cut(s) 521
CseI GACGC 1 cut(s) 442
Csp6I GTAC 2 cut(s) 280, 337
CviAII CATG 3 cut(s) 185, 357, 856
CviQI GTAC 2 cut(s) 280, 337
DdeI CTNAG 4 cut(s) 121, 150, 174, 873
DpnI GATC 3 cut(s) 520, 685, 909
DpnII GATC 3 cut(s) 518, 683, 907
DraIII CACNNNGTG 1 cut(s) 802
EciI GGCGGA 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 184, 729
Eco47I GGWCC 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 668
EcoT14I CCWWGG 2 cut(s) 184, 729
ErhI CCWWGG 2 cut(s) 184, 729
FaeI CATG 3 cut(s) 188, 360, 859
FalI AAGNNNNNCTT 2 cut(s) 565, 597
FaqI GGGAC 1 cut(s) 440
FatI CATG 3 cut(s) 184, 356, 855
FauI CCCGC 1 cut(s) 520
FblI GTMKAC 2 cut(s) 597, 865
Fnu4HI GCNGC 1 cut(s) 846
FokI GGATG 2 cut(s) 239, 625
Fsp4HI GCNGC 1 cut(s) 846
FspBI CTAG 3 cut(s) 489, 516, 918
GluI GCNGC 1 cut(s) 846
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 1 cut(s) 673
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 3 cut(s) 188, 360, 859
HincII GTYRAC 2 cut(s) 385, 866
HindII GTYRAC 2 cut(s) 385, 866
HinfI GANTC 7 cut(s) 189, 413, 430, 446, 605, 623, 921
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 1 cut(s) 222
HphI GGTGA 2 cut(s) 151, 465
Hpy166II GTNNAC 4 cut(s) 337, 385, 598, 866
Hpy188I TCNGA 5 cut(s) 153, 171, 616, 652, 876
Hpy188III TCNNGA 2 cut(s) 254, 417
Hpy8I GTNNAC 4 cut(s) 337, 385, 598, 866
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 109, 486, 556, 591
HpyCH4III ACNGT 4 cut(s) 9, 313, 382, 602
HpyCH4IV ACGT 3 cut(s) 509, 554, 888
HpyCH4V TGCA 4 cut(s) 78, 140, 328, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 84, 707
HpyF3I CTNAG 4 cut(s) 121, 150, 174, 873
HpySE526I ACGT 3 cut(s) 509, 554, 888
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 3 cut(s) 188, 360, 859
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 3 cut(s) 518, 683, 907
LmnI GCTCC 2 cut(s) 634, 805
Lsp1109I GCAGC 1 cut(s) 832
MaeI CTAG 3 cut(s) 489, 516, 918
MaeII ACGT 3 cut(s) 509, 554, 888
MaeIII GTNAC 3 cut(s) 376, 602, 634
MalI GATC 3 cut(s) 520, 685, 909
MboI GATC 3 cut(s) 518, 683, 907
MboII GAAGA 5 cut(s) 242, 416, 419, 422, 603
MluCI AATT 2 cut(s) 675, 768
MlyI GAGTC 3 cut(s) 198, 599, 632
MnlI CCTC 7 cut(s) 83, 92, 159, 218, 610, 771, 882
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 5 cut(s) 234, 384, 720, 884, 931
MslI CAYNNNNRTG 1 cut(s) 314
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 223, 670
MvaI CCWGG 1 cut(s) 670
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 40, 84, 707
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 184
NdeII GATC 3 cut(s) 518, 683, 907
NlaIII CATG 3 cut(s) 188, 360, 859
NlaIV GGNNCC 2 cut(s) 258, 630
NmuCI GTSAC 2 cut(s) 376, 602
NspV TTCGAA 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 4 cut(s) 413, 430, 446, 921
PflFI GACNNNGTC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 847
PleI GAGTC 3 cut(s) 197, 599, 631
PpsI GAGTC 3 cut(s) 197, 599, 631
Psp6I CCWGG 1 cut(s) 668
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 2 cut(s) 258, 630
PspPI GGNCC 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 175
RsaI GTAC 2 cut(s) 281, 338
RsaNI GTAC 2 cut(s) 280, 337
RseI CAYNNNNRTG 1 cut(s) 314
SalI GTCGAC 1 cut(s) 864
SaqAI TTAA 5 cut(s) 234, 384, 720, 884, 931
SatI GCNGC 1 cut(s) 846
Sau3AI GATC 3 cut(s) 518, 683, 907
Sau96I GGNCC 1 cut(s) 578
ScaI AGTACT 1 cut(s) 281
SchI GAGTC 3 cut(s) 198, 599, 632
ScrFI CCNGG 2 cut(s) 223, 670
SfcI CTRYAG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 550
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 314
Sse9I AATT 2 cut(s) 675, 768
SsiI CCGC 4 cut(s) 85, 527, 542, 861
SspMI CTAG 3 cut(s) 489, 516, 918
StyD4I CCNGG 2 cut(s) 221, 668
StyI CCWWGG 2 cut(s) 184, 729
TaaI ACNGT 4 cut(s) 9, 313, 382, 602
TaiI ACGT 3 cut(s) 512, 557, 891
TaqI TCGA 3 cut(s) 521, 550, 865
TasI AATT 2 cut(s) 675, 768
TatI WGTACW 2 cut(s) 279, 336
TfiI GAWTC 4 cut(s) 413, 430, 446, 921
Tru1I TTAA 5 cut(s) 234, 384, 720, 884, 931
Tru9I TTAA 5 cut(s) 234, 384, 720, 884, 931
TscAI CASTG 3 cut(s) 12, 316, 607
TseFI GTSAC 2 cut(s) 376, 602
TseI GCWGC 1 cut(s) 845
Tsp45I GTSAC 2 cut(s) 376, 602
TspDTI ATGAA 2 cut(s) 417, 575
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 3 cut(s) 12, 316, 607
Tth111I GACNNNGTC 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 578
XmiI GTMKAC 2 cut(s) 597, 865
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 3 cut(s) 489, 516, 918
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.