Rorug02G0109800

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005012
Physical Location & Seq
Reverse (-)
9444295 .. 9445668
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0109800.1

Sequence Viewer

Length: 807 bp
ATGACAATGATGCTAATGAAAGGAAACGGCCGGGGCTGGTTGATTCGATTGGATGCAGAACAAAAGTTACGTCCTCCATTATCGTGGAGTAATGGAGGTTTGGAGAAGCCGAGGGTAATAAACACAGTAGACTTTGGCGTACTTGAAGTGGCAAAACCTTATCTCTTGGCAAGTAATTACGACAAGAATTACCACAAGCTGGCTCTTTCCTTAAATCTCGACGTCTTCCCTGCAGTCATGCTGGTTAGGGATGGAGTTTTATACCTCGGAAAGTGGTCTGGTGGTGGTGTTGACGTTAAAGAATATTCCGTCCAACTCTCCCCGTCGAATCCTGATGATGATGATGATCCCTCATGCAGCAAAACAATTAAAGCTAATGATTATGATGATGTTATTTTTTATCATGGAAAGTTTTGTTCAGTCTCTCAGTCTGGGAAAGTTGCAGCGATGGATTCTTCCTTTAAGTGGAAAGTGATTGCGTTTCCAGTATCTACTTCAATGTGGTGGAAGAAGGAGAAGAAGAAGAACTTGGTCGAATCTCTCGGGGAGCTGCTTTTAGTCGAAAGACATGGTTCTACTTTTGTTGCAAAACATTGTTCTGTGGAAGATATGCTCAAGAATGAAGTCAAGTTCAAGGTTTACAAGTTGGATGGAGTTCGGAAGCAATGGGTTGAGATGAAGGGTTCGGATTTGGATGATCGAATATTGTTTGTGGGGGAAGACTGCTGTGGGGGAAGACTGCTGCTTTTCAGTCTCTACTCGAGACTTCCCGGGATGCCAAGGGAATTGCATATATTTTTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

268

Amino Acids

30.36

Weight (kDa)

8.45

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 113 - 243 1.6e-19 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccB7I CCANNNNNTGG 1 cut(s) 199
AccI GTMKAC 1 cut(s) 129
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 199, 465
AgsI TTSAA 3 cut(s) 146, 498, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 3 cut(s) 199, 374, 550
AluI AGCT 3 cut(s) 199, 374, 550
Alw26I GTCTC 3 cut(s) 427, 757, 758
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 3 cut(s) 542, 760, 770
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 4 cut(s) 357, 443, 550, 742
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AsuC2I CCSGG 3 cut(s) 32, 771, 772
AvaI CYCGRG 3 cut(s) 542, 760, 770
BbsI GAAGAC 3 cut(s) 217, 726, 742
BbvI GCAGC 4 cut(s) 369, 455, 537, 729
BccI CCATC 3 cut(s) 245, 442, 644
BceAI ACGGC 1 cut(s) 43
BcnI CCSGG 3 cut(s) 32, 771, 772
BcoDI GTCTC 3 cut(s) 427, 757, 758
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 4 cut(s) 358, 444, 551, 743
BlsI GCNGC 4 cut(s) 359, 445, 552, 744
Bme1390I CCNGG 3 cut(s) 32, 771, 772
BmeT110I CYCGRG 3 cut(s) 542, 760, 770
BmrFI CCNGG 3 cut(s) 32, 771, 772
BmsI GCATC 2 cut(s) 43, 765
BpiI GAAGAC 3 cut(s) 217, 726, 742
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 3 cut(s) 32, 771, 772
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 5 cut(s) 31, 110, 265, 770, 779
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 465
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 671
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 31, 110, 265, 770, 779
BseGI GGATG 5 cut(s) 58, 256, 655, 700, 780
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 199, 465
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 485
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 4 cut(s) 369, 455, 537, 729
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 31
BsiHKCI CYCGRG 3 cut(s) 542, 760, 770
BsiSI CCGG 2 cut(s) 31, 771
BslI CCNNNNNNNGG 2 cut(s) 199, 465
BsmAI GTCTC 3 cut(s) 427, 757, 758
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 3 cut(s) 542, 760, 770
Bsp143I GATC 2 cut(s) 346, 697
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 1 cut(s) 439
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 5 cut(s) 31, 110, 265, 770, 779
BssMI GATC 2 cut(s) 346, 697
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 779
Bst4CI ACNGT 1 cut(s) 127
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 426
BstF5I GGATG 5 cut(s) 58, 256, 655, 700, 780
BstKTI GATC 2 cut(s) 349, 700
BstMAI GTCTC 3 cut(s) 427, 757, 758
BstMBI GATC 2 cut(s) 346, 697
BstMCI CGRYCG 1 cut(s) 31
BstSCI CCNGG 3 cut(s) 30, 769, 770
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 369, 455, 537, 729
BstV2I GAAGAC 3 cut(s) 217, 726, 742
BstXI CCANNNNNNTGG 1 cut(s) 84
BstZI CGGCCG 1 cut(s) 28
BsuRI GGCC 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 461
BtsCI GGATG 5 cut(s) 58, 256, 655, 700, 780
Cac8I GCNNGC 1 cut(s) 201
Cfr9I CCCGGG 1 cut(s) 770
Csp6I GTAC 1 cut(s) 140
CviAII CATG 4 cut(s) 238, 354, 404, 569
CviJI RGCY 7 cut(s) 30, 36, 109, 199, 203, 374, 550
CviKI_1 RGCY 7 cut(s) 30, 36, 109, 199, 203, 374, 550
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 1 cut(s) 426
DpnI GATC 2 cut(s) 348, 699
DpnII GATC 2 cut(s) 346, 697
EaeI YGGCCR 1 cut(s) 28
EagI CGGCCG 1 cut(s) 28
EclXI CGGCCG 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 779
Eco52I CGGCCG 1 cut(s) 28
Eco88I CYCGRG 3 cut(s) 542, 760, 770
EcoT14I CCWWGG 1 cut(s) 779
ErhI CCWWGG 1 cut(s) 779
FaeI CATG 4 cut(s) 241, 357, 407, 572
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 4 cut(s) 237, 353, 403, 568
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 358, 444, 551, 743
FokI GGATG 5 cut(s) 65, 263, 662, 707, 787
Fsp4HI GCNGC 4 cut(s) 358, 444, 551, 743
GluI GCNGC 4 cut(s) 358, 444, 551, 743
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 31, 771
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 241, 357, 407, 572
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 4 cut(s) 43, 328, 452, 536
HpaII CCGG 2 cut(s) 31, 771
Hpy166II GTNNAC 3 cut(s) 130, 292, 640
Hpy188I TCNGA 3 cut(s) 269, 660, 688
Hpy188III TCNNGA 4 cut(s) 218, 332, 616, 762
Hpy8I GTNNAC 3 cut(s) 130, 292, 640
Hpy99I CGWCG 2 cut(s) 224, 328
HpyAV CCTTC 2 cut(s) 505, 673
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 3 cut(s) 70, 222, 294
HpyCH4V TGCA 6 cut(s) 56, 233, 357, 443, 587, 790
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 3 cut(s) 70, 222, 294
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 241, 357, 407, 572
Kzo9I GATC 2 cut(s) 346, 697
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 4 cut(s) 369, 455, 537, 729
LweI GCATC 2 cut(s) 43, 765
MaeII ACGT 3 cut(s) 70, 222, 294
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 348, 699
MboI GATC 2 cut(s) 346, 697
MboII GAAGA 9 cut(s) 217, 447, 520, 529, 532, 535, 617, 731, 747
MluCI AATT 4 cut(s) 175, 187, 366, 785
MmeI TCCRAC 2 cut(s) 337, 627
MnlI CCTC 5 cut(s) 84, 89, 105, 275, 361
MseI TTAA 4 cut(s) 212, 297, 369, 462
MslI CAYNNNNRTG 1 cut(s) 82
MspI CCGG 2 cut(s) 31, 771
MspR9I CCNGG 3 cut(s) 32, 771, 772
NciI CCSGG 3 cut(s) 32, 771, 772
NdeII GATC 2 cut(s) 346, 697
NlaIII CATG 4 cut(s) 241, 357, 407, 572
NmeAIII GCCGAG 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 760
PfeI GAWTC 4 cut(s) 43, 328, 452, 536
PflMI CCANNNNNTGG 1 cut(s) 199
PkrI GCNGC 4 cut(s) 359, 445, 552, 744
PsrI GAACNNNNNNTAC 2 cut(s) 51, 83
PstI CTGCAG 1 cut(s) 235
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 1 cut(s) 82
SaqAI TTAA 4 cut(s) 212, 297, 369, 462
SatI GCNGC 4 cut(s) 358, 444, 551, 743
Sau3AI GATC 2 cut(s) 346, 697
ScrFI CCNGG 3 cut(s) 32, 771, 772
SfaNI GCATC 2 cut(s) 43, 765
SfcI CTRYAG 1 cut(s) 231
Sfr274I CTCGAG 1 cut(s) 760
SlaI CTCGAG 1 cut(s) 760
SmaI CCCGGG 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 82
SmlI CTYRAG 2 cut(s) 614, 760
SmoI CTYRAG 2 cut(s) 614, 760
Sse9I AATT 4 cut(s) 175, 187, 366, 785
SspI AATATT 2 cut(s) 305, 705
StyD4I CCNGG 3 cut(s) 30, 769, 770
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 3 cut(s) 73, 225, 297
TaqI TCGA 7 cut(s) 46, 219, 326, 534, 561, 700, 761
TasI AATT 4 cut(s) 175, 187, 366, 785
TfiI GAWTC 4 cut(s) 43, 328, 452, 536
Tru1I TTAA 4 cut(s) 212, 297, 369, 462
Tru9I TTAA 4 cut(s) 212, 297, 369, 462
TseI GCWGC 4 cut(s) 357, 443, 550, 742
TspDTI ATGAA 3 cut(s) 32, 636, 692
TspGWI ACGGA 1 cut(s) 298
TspMI CCCGGG 1 cut(s) 770
Van91I CCANNNNNTGG 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 760
XmaI CCCGGG 1 cut(s) 770
XmiI GTMKAC 1 cut(s) 129
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.