pycom15g23610

oxygen-evolving enhancer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013223
Physical Location & Seq
Reverse (-)
17768318 .. 17769846
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGGCAGCCTCCCTCCAAGCAGCTGCTACGCTCATGCAGCCGACCAAGGTCGGCGTGTCGGCGAGGACCAGTGTGCAGCTGAGGTCCTCTCAGAGTGTCTCCAAGGCCTTTGGCTTGGAGACATCTGCCTCTGCTAGGCTCACTTGCTCTTTGCAATCTGATCTCAAGGACTTGGCTCACAAGTGCCTTGATGCTTCTAAGATTGCTGGATTTGCTCTTGCTACTTCCGCCCTCGTCGTCTCGGGAGCAGGTGCAGAGGGATCTCCAAAGAGGCTGACCTTCGACGAAATCCAGAGCAAGACCTACTTGGAAGTGAAGGGAACCGGAACTGCCAACCAGTGCCCAACCATCGATGGAGGATCCGAAGCATTCGCGTTCAAGCCTGGCAAGTACACTGCCAAGAAGTTCTGCCTAGAGCCAACATCCTTCACAGTCAAGGCAGAGAGTGTGAGCAAGAATGCTCCACCAGATTTCCAAAACACCAAGCTCATGACCCGCCTAACCTACACTCTGGATGAGATCGAAGGACCTTTCGAAGTTGCCCCAGATGGCACCGTCAAGTTTGAAGAGAAAGACGGAATTGACTACGCTGCAGTGACAGTCCAGCTCCCAGGAGGTGAGCGGGTGCCCTTCCTCTTTACCATCAAGCAGCTCGTAGCCTCTGGCAAGCCTGAAAGCTTCACAGGAGAGTTTCTAGTACCATCATACCGCGGATCCTCTTTCTTGGACCCCAAGGGAAGAGGAGGATCCACCGGTTATGACAATGCGGTTGCGTTGCCGGCAGGTGGCAGAGGAGACGAGGAGGAGTTGGCAAAGGAGAACACCAAGAATACAGCAGCATCATCAGGGAAGATCACACTGAGTGTGACAAAGAGCAAGCCTGAGACTGGTGAGTTGATTGGGGTGTTTGAGAGCATTCAGCCGTCTGACACTGACTTGGGAGCCAAGACTCCAAAGGATGTCAAAATCCAGGGAGTTTGGTATGCTCAGCTTGATTAG

Protein Analysis

333

Amino Acids

34.99

Weight (kDa)

5.75

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSP PF01716 99 - 331 1.7e-98 Manganese-stabilising protein / photosystem II polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50820 AT5G66570
fragaria_vesca FvH4_1g14430
malus_domestica MD02G1157800.v1.1 MD15G1272500.v1.1
prunus_persica Prupe.7G145900_v2.0.a1
pyrus_communis pycom02g12470 pycom15g23610
rosa_chinensis RchiOBHm_Chr2g0103161
rosa_laevigata RLG00000017214
rosa_multiflora Rmu_sc0000388.1_g000004
rosa_roxburghii Rroxscaffold_2G00139880
rosa_rugosa Rorug02G0112200
rosa_samantha Rh2AG161000 Rh2BG167700 Rh2CG168000 Rh2DG166600
rosa_wichuraiana Rw2G012630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 773
Acc36I ACCTGC 2 cut(s) 239, 773
AccB1I GGYRCC 2 cut(s) 551, 625
AccBSI CCGCTC 1 cut(s) 622
AccII CGCG 2 cut(s) 374, 711
AciI CCGC 6 cut(s) 228, 496, 622, 709, 711, 767
AclWI GGATC 7 cut(s) 268, 354, 367, 708, 721, 741, 754
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 2 cut(s) 392, 699
AfiI CCNNNNNNNGG 3 cut(s) 135, 785, 887
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 2 cut(s) 379, 566
AjnI CCWGG 3 cut(s) 382, 610, 969
AluBI AGCT 7 cut(s) 23, 79, 487, 607, 652, 678, 991
AluI AGCT 7 cut(s) 23, 79, 487, 607, 652, 678, 991
Alw26I GTCTC 5 cut(s) 103, 113, 244, 789, 878
AlwI GGATC 7 cut(s) 268, 354, 367, 708, 721, 741, 754
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 8 cut(s) 5, 20, 23, 37, 76, 590, 649, 836
AsiGI ACCGGT 1 cut(s) 752
AspS9I GGNCC 4 cut(s) 66, 84, 527, 727
AsuHPI GGTGA 2 cut(s) 629, 902
AsuII TTCGAA 1 cut(s) 534
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 4 cut(s) 66, 84, 527, 727
BaeGI GKGCMC 2 cut(s) 344, 630
BamHI GGATCC 3 cut(s) 359, 713, 746
BanI GGYRCC 2 cut(s) 551, 625
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 8 cut(s) 10, 17, 32, 49, 88, 577, 661, 848
BccI CCATC 5 cut(s) 347, 356, 542, 650, 709
BceAI ACGGC 1 cut(s) 907
BciT130I CCWGG 3 cut(s) 384, 612, 971
BcoDI GTCTC 5 cut(s) 103, 113, 244, 789, 878
BfaI CTAG 3 cut(s) 135, 413, 695
BfmI CTRYAG 1 cut(s) 591
BfuAI ACCTGC 2 cut(s) 239, 773
BisI GCNGC 8 cut(s) 6, 21, 24, 38, 77, 591, 650, 837
BlpI GCTNAGC 1 cut(s) 987
BlsI GCNGC 8 cut(s) 7, 22, 25, 39, 78, 592, 651, 838
Bme1390I CCNGG 3 cut(s) 384, 612, 971
Bme18I GGWCC 4 cut(s) 66, 84, 527, 727
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 66, 84, 527, 727
BmiI GGNNCC 8 cut(s) 322, 361, 553, 627, 715, 729, 748, 943
BmrFI CCNGG 3 cut(s) 384, 612, 971
BmsI GCATC 2 cut(s) 181, 848
BoxI GACNNNNGTC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 73, 105
Bpu10I CCTNAGC 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 351
BsaJI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BsaWI WCCGGW 2 cut(s) 323, 752
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 785, 887
Bse118I RCCGGY 2 cut(s) 752, 778
Bse1I ACTGG 3 cut(s) 69, 337, 892
BseBI CCWGG 3 cut(s) 384, 612, 971
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BseGI GGATG 3 cut(s) 422, 520, 964
BseLI CCNNNNNNNGG 3 cut(s) 135, 785, 887
BseMII CTCAG 4 cut(s) 71, 104, 851, 873
BseNI ACTGG 3 cut(s) 69, 337, 892
BseRI GAGGAG 4 cut(s) 756, 807, 815, 818
BseSI GKGCMC 2 cut(s) 344, 630
BseXI GCAGC 8 cut(s) 10, 17, 32, 49, 88, 577, 661, 848
BsgI GTGCAG 2 cut(s) 95, 273
Bsh1236I CGCG 2 cut(s) 374, 711
BshFI GGCC 1 cut(s) 107
BshNI GGYRCC 2 cut(s) 551, 625
BshTI ACCGGT 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 3 cut(s) 324, 753, 779
BslI CCNNNNNNNGG 3 cut(s) 135, 785, 887
BsmAI GTCTC 5 cut(s) 103, 113, 244, 789, 878
BsmBI CGTCTC 2 cut(s) 244, 789
BsmI GAATGC 3 cut(s) 368, 463, 915
BsnI GGCC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 344, 630
Bsp143I GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
Bsp1720I GCTNAGC 1 cut(s) 987
BspACI CCGC 6 cut(s) 228, 496, 622, 709, 711, 767
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 4 cut(s) 72, 103, 852, 874
BspDI ATCGAT 1 cut(s) 351
BspFNI CGCG 2 cut(s) 374, 711
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 8 cut(s) 322, 361, 553, 627, 715, 729, 748, 943
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 2 cut(s) 239, 773
BspPI GGATC 7 cut(s) 268, 354, 367, 708, 721, 741, 754
BspT104I TTCGAA 1 cut(s) 534
BspT107I GGYRCC 2 cut(s) 551, 625
BsrBI CCGCTC 1 cut(s) 622
BsrFI RCCGGY 2 cut(s) 752, 778
BsrI ACTGG 3 cut(s) 69, 337, 892
BssAI RCCGGY 2 cut(s) 752, 778
BssECI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BssMI GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
BssT1I CCWWGG 3 cut(s) 45, 102, 732
Bst2UI CCWGG 3 cut(s) 384, 612, 971
Bst4CI ACNGT 3 cut(s) 433, 556, 601
Bst6I CTCTTC 2 cut(s) 561, 733
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 668, 780, 878
BstDEI CTNAG 6 cut(s) 80, 90, 198, 860, 882, 987
BstDSI CCRYGG 1 cut(s) 709
BstENI CCTNNNNNAGG 1 cut(s) 133
BstF5I GGATG 3 cut(s) 422, 520, 964
BstFNI CGCG 2 cut(s) 374, 711
BstKTI GATC 7 cut(s) 163, 263, 362, 522, 716, 749, 855
BstMAI GTCTC 5 cut(s) 103, 113, 244, 789, 878
BstMBI GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
BstMWI GCNNNNNNNGC 4 cut(s) 37, 212, 227, 779
BstNI CCWGG 3 cut(s) 384, 612, 971
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 3 cut(s) 382, 610, 969
BstSFI CTRYAG 1 cut(s) 591
BstSLI GKGCMC 2 cut(s) 344, 630
BstUI CGCG 2 cut(s) 374, 711
BstV1I GCAGC 8 cut(s) 10, 17, 32, 49, 88, 577, 661, 848
BstX2I RGATCY 4 cut(s) 260, 359, 713, 746
BstYI RGATCY 4 cut(s) 260, 359, 713, 746
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 1 cut(s) 107
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 3 cut(s) 422, 520, 964
BtsI GCAGTG 2 cut(s) 393, 600
BtsIMutI CAGTG 6 cut(s) 76, 344, 393, 600, 857, 930
BveI ACCTGC 2 cut(s) 239, 773
Cac8I GCNNGC 3 cut(s) 668, 780, 878
CciI TCATGA 1 cut(s) 489
Cfr10I RCCGGY 2 cut(s) 752, 778
Cfr13I GGNCC 4 cut(s) 66, 84, 527, 727
Cfr42I CCGCGG 1 cut(s) 712
ClaI ATCGAT 1 cut(s) 351
Csp6I GTAC 2 cut(s) 391, 698
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 2 cut(s) 34, 490
CviQI GTAC 2 cut(s) 391, 698
DdeI CTNAG 6 cut(s) 80, 90, 198, 860, 882, 987
DpnI GATC 7 cut(s) 162, 262, 361, 521, 715, 748, 854
DpnII GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
DraIII CACNNNGTG 1 cut(s) 863
Eam1104I CTCTTC 2 cut(s) 561, 733
EarI CTCTTC 2 cut(s) 561, 733
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 45, 102, 732
Eco147I AGGCCT 1 cut(s) 107
Eco47I GGWCC 4 cut(s) 66, 84, 527, 727
Eco88I CYCGRG 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 84, 527
EcoRII CCWGG 3 cut(s) 382, 610, 969
EcoT14I CCWWGG 3 cut(s) 45, 102, 732
ErhI CCWWGG 3 cut(s) 45, 102, 732
Esp3I CGTCTC 2 cut(s) 244, 789
FaeI CATG 2 cut(s) 37, 493
FaiI YATR 5 cut(s) 35, 491, 706, 759, 984
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 2 cut(s) 33, 489
FauI CCCGC 2 cut(s) 503, 615
Fnu4HI GCNGC 8 cut(s) 6, 21, 24, 38, 77, 591, 650, 837
FokI GGATG 3 cut(s) 409, 527, 971
Fsp4HI GCNGC 8 cut(s) 6, 21, 24, 38, 77, 591, 650, 837
FspBI CTAG 3 cut(s) 135, 413, 695
GluI GCNGC 8 cut(s) 6, 21, 24, 38, 77, 591, 650, 837
HaeIII GGCC 1 cut(s) 107
HapII CCGG 3 cut(s) 324, 753, 779
Hin1II CATG 2 cut(s) 37, 493
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 1 cut(s) 949
HpaII CCGG 3 cut(s) 324, 753, 779
HphI GGTGA 2 cut(s) 629, 902
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 4 cut(s) 93, 160, 364, 928
Hpy188III TCNNGA 4 cut(s) 243, 292, 490, 512
Hpy8I GTNNAC 1 cut(s) 393
Hpy99I CGWCG 2 cut(s) 239, 287
HpyAV CCTTC 5 cut(s) 289, 310, 436, 518, 640
HpyCH4III ACNGT 3 cut(s) 433, 556, 601
HpyCH4V TGCA 5 cut(s) 37, 76, 154, 254, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 212, 227, 779
HpyF3I CTNAG 6 cut(s) 80, 90, 198, 860, 882, 987
Hsp92II CATG 2 cut(s) 37, 493
KroI GCCGGC 1 cut(s) 778
KroNI GCCGGC 1 cut(s) 780
KspI CCGCGG 1 cut(s) 712
Kzo9I GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
LmnI GCTCC 4 cut(s) 245, 466, 612, 941
Lsp1109I GCAGC 8 cut(s) 10, 17, 32, 49, 88, 577, 661, 848
LweI GCATC 2 cut(s) 181, 848
MaeI CTAG 3 cut(s) 135, 413, 695
MaeIII GTNAC 2 cut(s) 595, 865
MalI GATC 7 cut(s) 162, 262, 361, 521, 715, 748, 854
MbiI CCGCTC 1 cut(s) 622
MboI GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
MboII GAAGA 3 cut(s) 578, 750, 862
MflI RGATCY 4 cut(s) 260, 359, 713, 746
MhlI GDGCHC 2 cut(s) 344, 630
MluCI AATT 1 cut(s) 579
MlyI GAGTC 1 cut(s) 943
MroNI GCCGGC 1 cut(s) 778
MspA1I CMGCKG 3 cut(s) 23, 79, 711
MspI CCGG 3 cut(s) 324, 753, 779
MspR9I CCNGG 3 cut(s) 384, 612, 971
Mva1269I GAATGC 3 cut(s) 368, 463, 915
MvaI CCWGG 3 cut(s) 384, 612, 971
MvnI CGCG 2 cut(s) 374, 711
MwoI GCNNNNNNNGC 4 cut(s) 37, 212, 227, 779
NaeI GCCGGC 1 cut(s) 780
NdeII GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
NgoMIV GCCGGC 1 cut(s) 778
NlaIII CATG 2 cut(s) 37, 493
NlaIV GGNNCC 8 cut(s) 322, 361, 553, 627, 715, 729, 748, 943
NmuCI GTSAC 2 cut(s) 595, 865
NspV TTCGAA 1 cut(s) 534
PagI TCATGA 1 cut(s) 489
PaqCI CACCTGC 2 cut(s) 239, 773
PceI AGGCCT 1 cut(s) 107
PctI GAATGC 3 cut(s) 368, 463, 915
PdiI GCCGGC 1 cut(s) 780
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 8 cut(s) 7, 22, 25, 39, 78, 592, 651, 838
PleI GAGTC 1 cut(s) 943
PpsI GAGTC 1 cut(s) 943
PpuMI RGGWCCY 2 cut(s) 84, 527
PshAI GACNNNNGTC 1 cut(s) 47
Psp5II RGGWCCY 2 cut(s) 84, 527
Psp6I CCWGG 3 cut(s) 382, 610, 969
PspGI CCWGG 3 cut(s) 382, 610, 969
PspN4I GGNNCC 8 cut(s) 322, 361, 553, 627, 715, 729, 748, 943
PspPI GGNCC 4 cut(s) 66, 84, 527, 727
PspPPI RGGWCCY 2 cut(s) 84, 527
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 4 cut(s) 260, 359, 713, 746
PvuII CAGCTG 2 cut(s) 23, 79
RsaI GTAC 2 cut(s) 392, 699
RsaNI GTAC 2 cut(s) 391, 698
SacII CCGCGG 1 cut(s) 712
SatI GCNGC 8 cut(s) 6, 21, 24, 38, 77, 591, 650, 837
Sau3AI GATC 7 cut(s) 160, 260, 359, 519, 713, 746, 852
Sau96I GGNCC 4 cut(s) 66, 84, 527, 727
SchI GAGTC 1 cut(s) 943
ScrFI CCNGG 3 cut(s) 384, 612, 971
SduI GDGCHC 2 cut(s) 344, 630
SfaNI GCATC 2 cut(s) 181, 848
SfcI CTRYAG 1 cut(s) 591
Sfr303I CCGCGG 1 cut(s) 712
SfuI TTCGAA 1 cut(s) 534
SgrBI CCGCGG 1 cut(s) 712
SinI GGWCC 4 cut(s) 66, 84, 527, 727
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 1 cut(s) 579
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 6 cut(s) 228, 496, 622, 709, 711, 767
SspMI CTAG 3 cut(s) 135, 413, 695
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 382, 610, 969
StyI CCWWGG 3 cut(s) 45, 102, 732
TaaI ACNGT 3 cut(s) 433, 556, 601
TaqI TCGA 4 cut(s) 282, 351, 522, 534
TasI AATT 1 cut(s) 579
TatI WGTACW 1 cut(s) 390
TscAI CASTG 6 cut(s) 76, 344, 400, 600, 864, 937
TseFI GTSAC 2 cut(s) 595, 865
TseI GCWGC 8 cut(s) 5, 20, 23, 37, 76, 590, 649, 836
Tsp45I GTSAC 2 cut(s) 595, 865
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 6 cut(s) 76, 344, 400, 600, 864, 937
VpaK11BI GGWCC 4 cut(s) 66, 84, 527, 727
XagI CCTNNNNNAGG 1 cut(s) 133
XspI CTAG 3 cut(s) 135, 413, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.