pycom15g29660

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014574
Physical Location & Seq
Forward (+)
26578536 .. 26579128
593 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGGGAGTCGGAGGAACATTGGAGTACTTGTCTGATCTGGTGAACAACAGTGGCCACAAACACAAAAAGAAGAAGCAATTACAGACAGTGGAGCTCAAAATCAGGATGGACTGTGACGGCTGTGAGCTTAAGGTCAAGAATGCCCTCTCTTCCTTAACTGGAGTTAAATCTGTGGAGATAAACAGGAAGCAACAGAAGGTGAGTGTGACTGGGTATGTTGAACCAAACAAAGTGCTGAAGAAGGCAAAGTCAACAGGGAAGAGGGCAGAGATATGGCCTTATGTGCCCTACAACTTAGTGGCCCAGCCCTACATTGCTCAGGCTTATGACAAGAAGGCACCTCCTGGTTATGTCAGAAATGTGGACAACAATCCCTCCTCCACTGGGACTGCCACAAAATATGAAGACCCTTACACTACCATGTTCAGTGATGACAACCCTAATGCCTGCTCTATCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

154

Amino Acids

16.98

Weight (kDa)

9.33

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 87 7.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39700
fragaria_vesca FvH4_1g21640
malus_domestica MD01G1024200.v1.1 MD15G1328000.v1.1
prunus_persica Prupe.6G179300_v2.0.a1
pyrus_communis pycom01g05190 pycom15g29660
rosa_chinensis RchiOBHm_Chr2g0115511
rosa_laevigata RLG00000018189
rosa_multiflora Rmu_sc0008334.1_g000016
rosa_roxburghii Rroxscaffold_2G00129020
rosa_rugosa Rorug02G0197200
rosa_samantha Rh2AG254800 Rh2BG265500 Rh2CG260000 Rh2DG262900
rosa_wichuraiana Rw2G019940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 384
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 2 cut(s) 94, 127
AluI AGCT 2 cut(s) 94, 127
Alw21I GWGCWC 1 cut(s) 96
AoxI GGCC 3 cut(s) 52, 275, 300
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 2 cut(s) 52, 211
BaeGI GKGCMC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 1 cut(s) 100
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 345
BfrI CTTAAG 1 cut(s) 128
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 2 cut(s) 219, 393
BmuI ACTGGG 2 cut(s) 219, 393
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 318
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 3 cut(s) 163, 214, 388
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 3 cut(s) 163, 214, 388
BseRI GAGGAG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 288
BseYI CCCAGC 1 cut(s) 303
BshFI GGCC 3 cut(s) 54, 277, 302
BshNI GGYRCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 3 cut(s) 54, 277, 302
Bsp1286I GDGCHC 2 cut(s) 96, 288
Bsp143I GATC 1 cut(s) 34
BspANI GGCC 3 cut(s) 54, 277, 302
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 337
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 312
BsrI ACTGG 3 cut(s) 163, 214, 388
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 50, 88, 113
Bst6I CTCTTC 2 cut(s) 154, 254
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 2 cut(s) 295, 318
BstF5I GGATG 1 cut(s) 111
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 411
BsuRI GGCC 3 cut(s) 54, 277, 302
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 55, 93, 381, 433
Cac8I GCNNGC 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 301
Csp6I GTAC 1 cut(s) 25
CviAII CATG 2 cut(s) 421, 457
CviJI RGCY 8 cut(s) 54, 94, 120, 127, 277, 302, 307, 323
CviKI_1 RGCY 8 cut(s) 54, 94, 120, 127, 277, 302, 307, 323
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 295, 318
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 154, 254
EarI CTCTTC 2 cut(s) 154, 254
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 2 cut(s) 424, 460
FaiI YATR 8 cut(s) 216, 274, 282, 327, 351, 402, 422, 458
FaqI GGGAC 1 cut(s) 400
FatI CATG 2 cut(s) 420, 456
FokI GGATG 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 3 cut(s) 54, 277, 302
Hin1II CATG 2 cut(s) 424, 460
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 1 cut(s) 6
HphI GGTGA 2 cut(s) 52, 211
Hpy166II GTNNAC 3 cut(s) 43, 252, 364
Hpy188I TCNGA 3 cut(s) 11, 34, 356
Hpy188III TCNNGA 2 cut(s) 103, 136
Hpy8I GTNNAC 3 cut(s) 43, 252, 364
HpyAV CCTTC 3 cut(s) 190, 235, 328
HpyCH4III ACNGT 3 cut(s) 50, 88, 113
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 2 cut(s) 295, 318
Hsp92II CATG 2 cut(s) 424, 460
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 1 cut(s) 91
MaeIII GTNAC 2 cut(s) 113, 205
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 82, 141, 250, 271, 416
MhlI GDGCHC 2 cut(s) 96, 288
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 54
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 6 cut(s) 5, 155, 255, 351, 385, 388
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
MseI TTAA 3 cut(s) 129, 155, 165
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 54
MspCI CTTAAG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 283
NdeII GATC 1 cut(s) 34
NlaIII CATG 2 cut(s) 424, 460
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 2 cut(s) 113, 205
PctI GAATGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 301
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 3 cut(s) 129, 155, 165
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 301
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 96, 288
SetI ASST 5 cut(s) 96, 129, 135, 201, 343
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 77
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 3 cut(s) 50, 88, 113
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 24
Tru1I TTAA 3 cut(s) 129, 155, 165
Tru9I TTAA 3 cut(s) 129, 155, 165
TscAI CASTG 4 cut(s) 55, 93, 388, 433
TseFI GTSAC 2 cut(s) 113, 205
Tsp45I GTSAC 2 cut(s) 113, 205
TspDTI ATGAA 1 cut(s) 417
TspRI CASTG 4 cut(s) 55, 93, 388, 433
Vha464I CTTAAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.