Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0015005

Physical Location & Seq

Genomic Coordinates Reverse (-)

28383826 .. 28388074

Gene Structure 4249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGAGTCGATGGAGTCGCCGGAGTTGCCGGAGTTGCCGGAGTTGCCGGAGTGTCCGGTGTGTTTGCAGAACTACGACAGCGAGTCCACAATTCCGCGCGTACTCGCGTGCGGCCACTCGGCGTGCGAGGCCTGCTTGGTCAGGCTGCCCGAGCGGTACCCCGAGACGATTCGCTGCCCGGCGTGCACCCAACTCGTCAAGTACCCTCCGCAGGGACCCACAGCTCTGCCCAAGAACATCGACCTCCTCTCTTTCTCCCTCTCACTAAACCCTAACCCTAATTCCGGTATTTCCCAAACACCCCATAAACAATCGGCCGATGGAGTTGGTAGTTTCTTGCCGTGCATTTGGTCCGACGAGTTTTACGTCACGTGGAAGGACTGGGTGCTTCCAAGCGACGCCGTTTCGGTGGAAACCGAGGTTGATGATGGAACGAGAGATGAGCTTTGTACTGTGTTGAAAGGAAGAACTGGTTCTGGTTTCGGGTCGGGTCGGGTTTGGTTTAGAGAGGACGAGAGTGTCAGCCTTTTTCGGGTCGGTTCGTTACCGGGTTCGGATAGTTCTGGGTTTGAATTCAGTTACACTGCAAGGGTTTTGAAGTGTTTGAGTGGAATGAGAGAGGAAGAGAGGAACGAAATGGGATTGCTGCTGAGAGTTTCTGTGAGGCATTGTAGGAGAGTGTCTAAAGTTTATGGCTTTTGGGGAAATTTGGAAGATGGGTTTCTGTATTTGGGGTGTGAGAGGCAAAATCGGAGTTTTTCGGGGAAGTTGGGAGCTGGGGAGGATGGTTTTACTAAAGATGGGTTGCCTGCATTTGCAATGATTGCTATGGAGGTGTGTGAGGTGGTGTCCGGTTTGAATTCAGAGGGGTTTGTTGCTGGATGTTTTGGTTTTTCGTGCTTTAGTTTCGATGATTTTGGTCACGTTGAGGTGGATTTGAATGAGGTGTTAGTGACAGGAAGGAAGGTATGGAGAAGTGTTGTAGATTCTGTTTCTGGTCGAATAGGAACTGAATCAACTGATGCTGAAGTATTGAAATCGGCATTTTGGAATTTGTTTAAAGATGGTGATTTTGTTAGCCCGGAGGTGTTGATTGAGCTGTTGCAGAAACAGGGCGTTGCAGTAGAATGTGATAGCTCGAGATATCCGGTTGGATGTGGCTCGGATGTTTGGTCATTAGCCTGTGTGTTTCTAAGACTGCTACTTGGAAAAGAGTTTGATGAAGAGCTTGTGAAGAATTGTGGAATCTCTTTTTTTGACCATGTAACTTATGTAAGCTGGATTGAAAGAGTCCGTGCTTTGATCGAAGGGAGATTGGGTCAGGAATATGCATCCCTAAGGGAAAACCTCTGCCAATGTTTGAATTATGATCCAGCAAGTCGCCCACTTGTGATGGACCTGATGAAATGCATCAGGGAACTGATTATTAAGCCTCAATGTGATATCATGGCCAGTTTGGAGGGGGTGATTAAGGAAGATAGTGGAAGCTTTTGCTTGATTCTGGGGCAACTCTGTGGGACACGCAAGGAAATATTAGAAACACCAAAAGAAAATGGACTGCAAGGAAGTGAAATCAGTGGAGGATCAGATTTTGATCAAGTTGGGGATGAGAGGGCTGACAGTGATTTTTTTAATGGCCTAGCAGGGGGAAAGGTCAAGTTTAAAGTTCTGCAGGGTCATCGTGATGCTATTACAGGGTTAGCTGTTGGAGGGGACTTTCTATTTAGCTCCTCATTCGATAAAACTATCCATGTATGGTCGTTGCAGGACTTCTCTCATGTACATACATTTAAGGGCCATGAACATACCATCAAGGCTCTAATTTATGTAGATGAAGAGCAACCCTTTTGCATTAGTGGTGACAGTGGAGGTGGTATATTTGTCTGGGGCACATGTACCCCTCTCCAGCAAGAGCCTTTAAAGACATTCCACGAGGATAAAGATTGGCGCTTTAGTGGCATTCATGCCTTGGCCTGCCAAAATGGATATGTCTATACTGGCAGTGGAGATAGAACAGTAAAGGCATGGTCAGTGCAGGATGGCACCTTGTCATGCACTATGTCTGGTCATAGATCAGTAGTTTCAACGCTTGCAGTTTGTGATGGTGTTCTTTATAGCGGTAGTTGGGATGGAACCATCAGATTATGGAGTCTTAGCGATCACAGTCCTTTGACAGTACTAGGCGAAGACACATCAGGAACCGTGGCATCTGTCTTATCTCTTGCTGTTGACAGGCACATGCTTATTGCAACTCATGACAATGGATGTGTAAAGGTCTGGAGGAATGATGTGTTCATGAAATCCATTAAAATGCACAACGGTGCAGTATTTGCTTCTGGCATGGAGGGAAAATGGCTTTTCACGGGAGGGTTGGATAAAACTGTCAATGTACAGGAATTGTCTGGCGACAAGTTTCAAATAGATTCGAGACTTATTGGATCCATCCCCTGTGATTCTGTCATAACAACTTTGTTAGGTTGGCAAGGAAAGCTTTTTGTTGGATGTGCTAATAGGAATATCGTGGTATCTTACTATGGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045159 GO:0046777 GO:0051301 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

WD40 zf-RING_2 zf-RING_UBOX

Protein Analysis

848

Amino Acids

93.08

Weight (kDa)

4.98

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_5	PF14634	17 - 63	6.3e-07	zinc-RING finger domain
zf-RING_2	PF13639	17 - 62	9.6e-06	Ring finger domain
zf-RING_UBOX	PF13445	18 - 60	1.6e-07	RING-type zinc-finger
WDR55	PF24796	549 - 760	1.9e-10	WDR55
Beta-prop_WDR3_1st	PF25173	555 - 629	2.8e-08	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	555 - 620	6e-06	CDC20/Fizzy WD40 domain
EIF3I	PF24805	556 - 633	9.7e-06	EIF3I
Beta-prop_WDR5	PF25175	557 - 801	3.8e-23	WDR5 beta-propeller domain
WD40_Prp19	PF24814	652 - 754	3.9e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	658 - 760	6.5e-08	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	660 - 797	1.3e-13	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	660 - 776	4.2e-07	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	661 - 724	5.6e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21651
fragaria_vesca	FvH4_2g27740
prunus_persica	Prupe.1G490500_v2.0.a1 Prupe.1G490500_v2.0.a1 Prupe.1G490500_v2.0.a1
pyrus_communis	pycom15g30580
rosa_chinensis	RchiOBHm_Chr6g0296591
rosa_laevigata	RLG00000011677
rosa_multiflora	Rmu_sc0001741.1_g000020
rosa_roxburghii	Rroxscaffold_6G00390100 Rroxscaffold_7G00178750
rosa_rugosa	Rorug06G0263600
rosa_samantha	Rh6BG383500 Rh6CG388600 Rh6DG376000
rosa_wichuraiana	Rw6G032710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	518
Acc65I	GGTACC	1 cut(s)	156
AccB1I	GGYRCC	2 cut(s)	156, 2042
AccBSI	CCGCTC	1 cut(s)	154
AccII	CGCG	3 cut(s)	97, 99, 107
AciI	CCGC	5 cut(s)	95, 111, 154, 209, 2118
AclWI	GGATC	4 cut(s)	1364, 1591, 2433, 2446
AcoI	YGGCCR	3 cut(s)	112, 315, 1449
AcsI	RAATTY	4 cut(s)	572, 706, 859, 1051
AcuI	CTGAAG	1 cut(s)	1047
AcvI	CACGTG	1 cut(s)	372
AcyI	GRCGYC	1 cut(s)	399
AfaI	GTAC	8 cut(s)	102, 158, 203, 451, 1782, 1897, 2178, 2391
AfiI	CCNNNNNNNGG	5 cut(s)	211, 212, 284, 532, 1645
AflIII	ACRYGT	1 cut(s)	1892
AhdI	GACNNNNNGTC	1 cut(s)	82
AleI	CACNNNNGTG	1 cut(s)	2319
Alw21I	GWGCWC	1 cut(s)	188
Alw26I	GTCTC	2 cut(s)	158, 2422
Alw44I	GTGCAC	1 cut(s)	184
AlwI	GGATC	4 cut(s)	1364, 1591, 2433, 2446
Ama87I	CYCGRG	3 cut(s)	149, 161, 1138
AoxI	GGCC	7 cut(s)	112, 129, 315, 1449, 1636, 1795, 1971
ApaLI	GTGCAC	1 cut(s)	184
ApeKI	GCWGC	3 cut(s)	145, 174, 646
ApoI	RAATTY	4 cut(s)	572, 706, 859, 1051
Asp700I	GAANNNNTTC	1 cut(s)	472
Asp718I	GGTACC	1 cut(s)	156
AspLEI	GCGC	2 cut(s)	99, 1950
AspS9I	GGNCC	4 cut(s)	215, 351, 1396, 1795
AsuC2I	CCSGG	3 cut(s)	179, 549, 1082
AsuHPI	GGTGA	3 cut(s)	1079, 1477, 1871
AvaI	CYCGRG	3 cut(s)	149, 161, 1138
AvaII	GGWCC	3 cut(s)	215, 351, 1396
AxyI	CCTNAGG	1 cut(s)	1337
BaeGI	GKGCMC	2 cut(s)	188, 1892
BalI	TGGCCA	1 cut(s)	1451
BamHI	GGATCC	1 cut(s)	2438
BanI	GGYRCC	2 cut(s)	156, 2042
BauI	CACGAG	1 cut(s)	1931
BbrPI	CACGTG	1 cut(s)	372
BbsI	GAAGAC	1 cut(s)	2193
Bbv12I	GWGCWC	1 cut(s)	188
BbvI	GCAGC	3 cut(s)	132, 161, 633
BceAI	ACGGC	2 cut(s)	325, 386
BcgI	CGANNNNNNTGC	4 cut(s)	175, 209, 1661, 1695
BclI	TGATCA	1 cut(s)	1594
BcnI	CCSGG	3 cut(s)	179, 549, 1082
BcoDI	GTCTC	2 cut(s)	158, 2422
BfaI	CTAG	2 cut(s)	1640, 2180
BfmI	CTRYAG	1 cut(s)	1670
BfoI	RGCGCY	1 cut(s)	1951
BisI	GCNGC	4 cut(s)	112, 146, 175, 647
BlsI	GCNGC	4 cut(s)	113, 147, 176, 648
BmcAI	AGTACT	1 cut(s)	2178
Bme1390I	CCNGG	3 cut(s)	179, 549, 1082
Bme18I	GGWCC	3 cut(s)	215, 351, 1396
BmeRI	GACNNNNNGTC	1 cut(s)	82
BmeT110I	CYCGRG	3 cut(s)	149, 161, 1138
BmgT120I	GGNCC	4 cut(s)	215, 351, 1396, 1795
BmiI	GGNNCC	7 cut(s)	158, 216, 217, 2044, 2134, 2200, 2440
BmrFI	CCNGG	3 cut(s)	179, 549, 1082
BmrI	ACTGGG	1 cut(s)	391
BmsI	GCATC	5 cut(s)	1012, 1340, 1419, 1675, 2216
BmuI	ACTGGG	1 cut(s)	391
BpiI	GAAGAC	1 cut(s)	2193
BpmI	CTGGAG	2 cut(s)	1889, 2299
BpuMI	CCSGG	3 cut(s)	179, 549, 1082
BsaAI	YACGTR	1 cut(s)	372
BsaBI	GATNNNNATC	1 cut(s)	1593
BsaHI	GRCGYC	1 cut(s)	399
BsaJI	CCNNGG	3 cut(s)	417, 1968, 2202
BsaWI	WCCGGW	4 cut(s)	55, 284, 851, 1147
BsaXI	ACNNNNNCTCC	2 cut(s)	1303, 1333
Bsc4I	CCNNNNNNNGG	5 cut(s)	211, 212, 284, 532, 1645
Bse1I	ACTGG	4 cut(s)	386, 475, 1452, 2002
Bse21I	CCTNAGG	1 cut(s)	1337
Bse3DI	GCAATG	1 cut(s)	825
Bse8I	GATNNNNATC	1 cut(s)	1593
BseDI	CCNNGG	3 cut(s)	417, 1968, 2202
BseJI	GATNNNNATC	1 cut(s)	1593
BseLI	CCNNNNNNNGG	5 cut(s)	211, 212, 284, 532, 1645
BseMI	GCAATG	1 cut(s)	825
BseMII	CTCAG	1 cut(s)	641
BseNI	ACTGG	4 cut(s)	386, 475, 1452, 2002
BseRI	GAGGAG	2 cut(s)	236, 1720
BseSI	GKGCMC	2 cut(s)	188, 1892
BseX3I	CGGCCG	1 cut(s)	315
BseXI	GCAGC	3 cut(s)	132, 161, 633
BseYI	CCCAGC	1 cut(s)	776
BsgI	GTGCAG	2 cut(s)	2054, 2343
Bsh1236I	CGCG	3 cut(s)	97, 99, 107
Bsh1285I	CGRYCG	1 cut(s)	318
BshFI	GGCC	7 cut(s)	114, 131, 317, 1451, 1638, 1797, 1973
BshNI	GGYRCC	2 cut(s)	156, 2042
BsiEI	CGRYCG	1 cut(s)	318
BsiHKAI	GWGCWC	1 cut(s)	188
BsiHKCI	CYCGRG	3 cut(s)	149, 161, 1138
BslFI	GGGAC	3 cut(s)	228, 1531, 1727
BslI	CCNNNNNNNGG	5 cut(s)	211, 212, 284, 532, 1645
BsmAI	GTCTC	2 cut(s)	158, 2422
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	3 cut(s)	228, 1531, 1727
BsmI	GAATGC	1 cut(s)	1959
BsnI	GGCC	7 cut(s)	114, 131, 317, 1451, 1638, 1797, 1973
BsoBI	CYCGRG	3 cut(s)	149, 161, 1138
Bsp1286I	GDGCHC	2 cut(s)	188, 1892
Bsp1407I	TGTACA	2 cut(s)	1780, 2389
Bsp143I	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
BspACI	CCGC	5 cut(s)	95, 111, 154, 209, 2118
BspANI	GGCC	7 cut(s)	114, 131, 317, 1451, 1638, 1797, 1973
BspCNI	CTCAG	1 cut(s)	642
BspFNI	CGCG	3 cut(s)	97, 99, 107
BspHI	TCATGA	2 cut(s)	2254, 2295
BspLI	GGNNCC	7 cut(s)	158, 216, 217, 2044, 2134, 2200, 2440
BspMAI	CTGCAG	1 cut(s)	1674
BspPI	GGATC	4 cut(s)	1364, 1591, 2433, 2446
BspQI	GCTCTTC	2 cut(s)	1218, 1830
BspT107I	GGYRCC	2 cut(s)	156, 2042
BsrBI	CCGCTC	1 cut(s)	154
BsrDI	GCAATG	1 cut(s)	825
BsrGI	TGTACA	2 cut(s)	1780, 2389
BsrI	ACTGG	4 cut(s)	386, 475, 1452, 2002
BssECI	CCNNGG	3 cut(s)	417, 1968, 2202
BssMI	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
BssNI	GRCGYC	1 cut(s)	399
BssSI	CACGAG	1 cut(s)	1931
BssT1I	CCWWGG	1 cut(s)	1968
Bst2BI	CACGAG	1 cut(s)	1931
Bst4CI	ACNGT	9 cut(s)	454, 1622, 1865, 2017, 2165, 2176, 2203, 2321, 2383
Bst6I	CTCTTC	3 cut(s)	618, 1218, 1830
BstACI	GRCGYC	1 cut(s)	399
BstAPI	GCANNNNNTGC	2 cut(s)	824, 2330
BstAUI	TGTACA	2 cut(s)	1780, 2389
BstBAI	YACGTR	1 cut(s)	372
BstC8I	GCNNGC	7 cut(s)	109, 124, 133, 184, 810, 1975, 2091
BstDEI	CTNAG	4 cut(s)	650, 1193, 1337, 2153
BstDSI	CCRYGG	1 cut(s)	2202
BstFNI	CGCG	3 cut(s)	97, 99, 107
BstH2I	RGCGCY	1 cut(s)	1951
BstHHI	GCGC	2 cut(s)	99, 1950
BstKTI	GATC	7 cut(s)	1305, 1372, 1586, 1597, 2075, 2161, 2441
BstMAI	GTCTC	2 cut(s)	158, 2422
BstMBI	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
BstMCI	CGRYCG	1 cut(s)	318
BstNSI	RCATGY	2 cut(s)	1896, 2242
BstSCI	CCNGG	3 cut(s)	177, 547, 1080
BstSFI	CTRYAG	1 cut(s)	1670
BstSLI	GKGCMC	2 cut(s)	188, 1892
BstUI	CGCG	3 cut(s)	97, 99, 107
BstV1I	GCAGC	3 cut(s)	132, 161, 633
BstV2I	GAAGAC	1 cut(s)	2193
BstX2I	RGATCY	1 cut(s)	2438
BstYI	RGATCY	1 cut(s)	2438
BstZI	CGGCCG	1 cut(s)	315
Bsu36I	CCTNAGG	1 cut(s)	1337
BsuRI	GGCC	7 cut(s)	114, 131, 317, 1451, 1638, 1797, 1973
BtgI	CCRYGG	1 cut(s)	2202
BtsI	GCAGTG	2 cut(s)	582, 2008
BtsIMutI	CAGTG	6 cut(s)	582, 1582, 1627, 1870, 2008, 2037
Cac8I	GCNNGC	7 cut(s)	109, 124, 133, 184, 810, 1975, 2091
CciI	TCATGA	2 cut(s)	2254, 2295
CfoI	GCGC	2 cut(s)	99, 1950
Cfr13I	GGNCC	4 cut(s)	215, 351, 1396, 1795
CseI	GACGC	1 cut(s)	407
Csp6I	GTAC	8 cut(s)	101, 157, 202, 450, 1781, 1896, 2177, 2390
CviQI	GTAC	8 cut(s)	101, 157, 202, 450, 1781, 1896, 2177, 2390
DdeI	CTNAG	4 cut(s)	650, 1193, 1337, 2153
DpnI	GATC	7 cut(s)	1304, 1371, 1585, 1596, 2074, 2160, 2440
DpnII	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
DraI	TTTAAA	3 cut(s)	1060, 1663, 1920
DrdI	GACNNNNNNGTC	1 cut(s)	518
DriI	GACNNNNNGTC	1 cut(s)	82
DseDI	GACNNNNNNGTC	1 cut(s)	518
EaeI	YGGCCR	3 cut(s)	112, 315, 1449
EagI	CGGCCG	1 cut(s)	315
Eam1104I	CTCTTC	3 cut(s)	618, 1218, 1830
Eam1105I	GACNNNNNGTC	1 cut(s)	82
EarI	CTCTTC	3 cut(s)	618, 1218, 1830
EclXI	CGGCCG	1 cut(s)	315
Eco130I	CCWWGG	1 cut(s)	1968
Eco147I	AGGCCT	1 cut(s)	131
Eco32I	GATATC	2 cut(s)	1145, 1444
Eco47I	GGWCC	3 cut(s)	215, 351, 1396
Eco52I	CGGCCG	1 cut(s)	315
Eco57I	CTGAAG	1 cut(s)	1047
Eco72I	CACGTG	1 cut(s)	372
Eco81I	CCTNAGG	1 cut(s)	1337
Eco88I	CYCGRG	3 cut(s)	149, 161, 1138
EcoO109I	RGGNCCY	1 cut(s)	215
EcoRI	GAATTC	2 cut(s)	572, 859
EcoRV	GATATC	2 cut(s)	1145, 1444
EcoT14I	CCWWGG	1 cut(s)	1968
EcoT22I	ATGCAT	2 cut(s)	1333, 1412
ErhI	CCWWGG	1 cut(s)	1968
Esp3I	CGTCTC	1 cut(s)	158
FalI	AAGNNNNNCTT	2 cut(s)	2475, 2507
FaqI	GGGAC	3 cut(s)	228, 1531, 1727
FbaI	TGATCA	1 cut(s)	1594
Fnu4HI	GCNGC	4 cut(s)	112, 146, 175, 647
Fsp4HI	GCNGC	4 cut(s)	112, 146, 175, 647
FspBI	CTAG	2 cut(s)	1640, 2180
GlaI	GCGC	2 cut(s)	98, 1949
GluI	GCNGC	4 cut(s)	112, 146, 175, 647
GsaI	CCCAGC	1 cut(s)	780
GsuI	CTGGAG	2 cut(s)	1889, 2299
HaeII	RGCGCY	1 cut(s)	1951
HaeIII	GGCC	7 cut(s)	114, 131, 317, 1451, 1638, 1797, 1973
HgaI	GACGC	1 cut(s)	407
HhaI	GCGC	2 cut(s)	99, 1950
Hin1I	GRCGYC	1 cut(s)	399
Hin6I	GCGC	2 cut(s)	97, 1948
HinP1I	GCGC	2 cut(s)	97, 1948
HincII	GTYRAC	1 cut(s)	2230
HindII	GTYRAC	1 cut(s)	2230
HindIII	AAGCTT	2 cut(s)	1486, 2489
HphI	GGTGA	3 cut(s)	1079, 1477, 1871
Hpy166II	GTNNAC	3 cut(s)	87, 186, 2230
Hpy188I	TCNGA	7 cut(s)	355, 556, 753, 865, 1165, 1588, 2141
Hpy188III	TCNNGA	8 cut(s)	1140, 1322, 1682, 2196, 2255, 2278, 2296, 2427
Hpy8I	GTNNAC	3 cut(s)	87, 186, 2230
Hpy99I	CGWCG	2 cut(s)	359, 401
HpyAV	CCTTC	4 cut(s)	370, 954, 958, 1301
HpyCH4III	ACNGT	9 cut(s)	454, 1622, 1865, 2017, 2165, 2176, 2203, 2321, 2383
HpyCH4IV	ACGT	3 cut(s)	366, 371, 924
HpyF3I	CTNAG	4 cut(s)	650, 1193, 1337, 2153
HpySE526I	ACGT	3 cut(s)	366, 371, 924
Hsp92I	GRCGYC	1 cut(s)	399
HspAI	GCGC	2 cut(s)	97, 1948
KflI	GGGWCCC	1 cut(s)	215
KpnI	GGTACC	1 cut(s)	160
Ksp22I	TGATCA	1 cut(s)	1594
Kzo9I	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
LguI	GCTCTTC	2 cut(s)	1218, 1830
LmnI	GCTCC	2 cut(s)	773, 1733
Lsp1109I	GCAGC	3 cut(s)	132, 161, 633
LweI	GCATC	5 cut(s)	1012, 1340, 1419, 1675, 2216
MaeI	CTAG	2 cut(s)	1640, 2180
MaeII	ACGT	3 cut(s)	366, 371, 924
MaeIII	GTNAC	7 cut(s)	367, 543, 578, 920, 952, 1264, 1859
MalI	GATC	7 cut(s)	1304, 1371, 1585, 1596, 2074, 2160, 2440
MbiI	CCGCTC	1 cut(s)	154
MboI	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
MboII	GAAGA	8 cut(s)	477, 635, 725, 1235, 1246, 1487, 1847, 2198
MflI	RGATCY	1 cut(s)	2438
MhlI	GDGCHC	2 cut(s)	188, 1892
MlsI	TGGCCA	1 cut(s)	1451
MluNI	TGGCCA	1 cut(s)	1451
MlyI	GAGTC	5 cut(s)	14, 23, 92, 1299, 2158
MmeI	TCCRAC	5 cut(s)	378, 1132, 1687, 2352, 2479
Mox20I	TGGCCA	1 cut(s)	1451
Mph1103I	ATGCAT	2 cut(s)	1333, 1412
MroXI	GAANNNNTTC	1 cut(s)	472
MscI	TGGCCA	1 cut(s)	1451
MseI	TTAA	8 cut(s)	1059, 1428, 1470, 1632, 1662, 1791, 1919, 2307
MslI	CAYNNNNRTG	4 cut(s)	1683, 2259, 2309, 2319
Msp20I	TGGCCA	1 cut(s)	1451
MspR9I	CCNGG	3 cut(s)	179, 549, 1082
Mva1269I	GAATGC	1 cut(s)	1959
MvnI	CGCG	3 cut(s)	97, 99, 107
NciI	CCSGG	3 cut(s)	179, 549, 1082
NdeII	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
NlaIV	GGNNCC	7 cut(s)	158, 216, 217, 2044, 2134, 2200, 2440
NmeAIII	GCCGAG	1 cut(s)	98
NmuCI	GTSAC	4 cut(s)	367, 920, 952, 1859
NsiI	ATGCAT	2 cut(s)	1333, 1412
NspI	RCATGY	2 cut(s)	1896, 2242
OliI	CACNNNNGTG	1 cut(s)	2319
PaeR7I	CTCGAG	1 cut(s)	1138
PagI	TCATGA	2 cut(s)	2254, 2295
PceI	AGGCCT	1 cut(s)	131
PciI	ACATGT	1 cut(s)	1892
PciSI	GCTCTTC	2 cut(s)	1218, 1830
PcsI	WCGNNNNNNNCGW	1 cut(s)	363
PctI	GAATGC	1 cut(s)	1959
PdmI	GAANNNNTTC	1 cut(s)	472
PfeI	GAWTC	7 cut(s)	169, 986, 1013, 1245, 1498, 2423, 2453
PkrI	GCNGC	4 cut(s)	113, 147, 176, 648
PleI	GAGTC	5 cut(s)	13, 22, 91, 1298, 2157
PmaCI	CACGTG	1 cut(s)	372
PmlI	CACGTG	1 cut(s)	372
PpsI	GAGTC	5 cut(s)	13, 22, 91, 1298, 2157
Ppu21I	YACGTR	1 cut(s)	372
PpuMI	RGGWCCY	1 cut(s)	215
PscI	ACATGT	1 cut(s)	1892
Psp5II	RGGWCCY	1 cut(s)	215
PspCI	CACGTG	1 cut(s)	372
PspFI	CCCAGC	1 cut(s)	776
PspN4I	GGNNCC	7 cut(s)	158, 216, 217, 2044, 2134, 2200, 2440
PspPI	GGNCC	4 cut(s)	215, 351, 1396, 1795
PspPPI	RGGWCCY	1 cut(s)	215
PstI	CTGCAG	1 cut(s)	1674
PsuI	RGATCY	1 cut(s)	2438
RsaI	GTAC	8 cut(s)	102, 158, 203, 451, 1782, 1897, 2178, 2391
RsaNI	GTAC	8 cut(s)	101, 157, 202, 450, 1781, 1896, 2177, 2390
RseI	CAYNNNNRTG	4 cut(s)	1683, 2259, 2309, 2319
SapI	GCTCTTC	2 cut(s)	1218, 1830
SaqAI	TTAA	8 cut(s)	1059, 1428, 1470, 1632, 1662, 1791, 1919, 2307
SatI	GCNGC	4 cut(s)	112, 146, 175, 647
Sau3AI	GATC	7 cut(s)	1302, 1369, 1583, 1594, 2072, 2158, 2438
Sau96I	GGNCC	4 cut(s)	215, 351, 1396, 1795
ScaI	AGTACT	1 cut(s)	2178
SchI	GAGTC	5 cut(s)	14, 23, 92, 1299, 2158
ScrFI	CCNGG	3 cut(s)	179, 549, 1082
SduI	GDGCHC	2 cut(s)	188, 1892
SfaNI	GCATC	5 cut(s)	1012, 1340, 1419, 1675, 2216
SfcI	CTRYAG	1 cut(s)	1670
Sfr274I	CTCGAG	1 cut(s)	1138
SinI	GGWCC	3 cut(s)	215, 351, 1396
SlaI	CTCGAG	1 cut(s)	1138
SmiMI	CAYNNNNRTG	4 cut(s)	1683, 2259, 2309, 2319
SmlI	CTYRAG	1 cut(s)	1138
SmoI	CTYRAG	1 cut(s)	1138
SseBI	AGGCCT	1 cut(s)	131
SsiI	CCGC	5 cut(s)	95, 111, 154, 209, 2118
SspI	AATATT	1 cut(s)	1533
SspMI	CTAG	2 cut(s)	1640, 2180
StuI	AGGCCT	1 cut(s)	131
StyD4I	CCNGG	3 cut(s)	177, 547, 1080
StyI	CCWWGG	1 cut(s)	1968
TaaI	ACNGT	9 cut(s)	454, 1622, 1865, 2017, 2165, 2176, 2203, 2321, 2383
TaiI	ACGT	3 cut(s)	369, 374, 927
TaqI	TCGA	8 cut(s)	8, 240, 909, 1000, 1139, 1305, 1737, 2426
TatI	WGTACW	4 cut(s)	449, 1780, 2176, 2389
TauI	GCSGC	1 cut(s)	114
TfiI	GAWTC	7 cut(s)	169, 986, 1013, 1245, 1498, 2423, 2453
Tru1I	TTAA	8 cut(s)	1059, 1428, 1470, 1632, 1662, 1791, 1919, 2307
Tru9I	TTAA	8 cut(s)	1059, 1428, 1470, 1632, 1662, 1791, 1919, 2307
TscAI	CASTG	6 cut(s)	589, 1582, 1627, 1870, 2008, 2037
TseFI	GTSAC	4 cut(s)	367, 920, 952, 1859
TseI	GCWGC	3 cut(s)	145, 174, 646
Tsp45I	GTSAC	4 cut(s)	367, 920, 952, 1859
TspDTI	ATGAA	7 cut(s)	1236, 1418, 1815, 1848, 1952, 2284, 2312
TspGWI	ACGGA	1 cut(s)	1283
TspRI	CASTG	6 cut(s)	589, 1582, 1627, 1870, 2008, 2037
VneI	GTGCAC	1 cut(s)	184
VpaK11BI	GGWCC	3 cut(s)	215, 351, 1396
XapI	RAATTY	4 cut(s)	572, 706, 859, 1051
XceI	RCATGY	2 cut(s)	1896, 2242
XhoI	CTCGAG	1 cut(s)	1138
XmnI	GAANNNNTTC	1 cut(s)	472
XspI	CTAG	2 cut(s)	1640, 2180
ZrmI	AGTACT	1 cut(s)	2178
Zsp2I	ATGCAT	2 cut(s)	1333, 1412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.