RchiOBHm_Chr6g0296591

SecA preprotein cross-linking domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015005
Physical Location & Seq
Forward (+)
58379768 .. 58384834
5067 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26622

Sequence Viewer

Length: 2397 bp
ATGGAGCTACCGGAGTGCCCGGTGTGTCTGCAGAACTACGACGGCGCGTCGACGATTCCGCGCGTGCTGGGTTGCGGCCACTCGGCGTGCGAGGTCTGCCTGGCGAAGCTGCCCCACCGCTACCCGGAGACGATTCGCTGCCCGGCGTGTACTCAGCTCGTCAAGTACCCTCAGAACGGCGCCGCCGCGCTGCCCAAGAACATCGACCTTCTCTCGCTCGCTCTCTCCCTCTCTCCGCCGCAAAACCCTAACCCCAATTCCGACGATCCCCGGAAGCCACGTCAGCAATCCGCGGATGATTTAGAAGTGTTTAATTTCCTGCCGCGGCTATGGTCCGACGAGTTTTATAGTGCTTGGAAGGACTGGGTTGTTGGGTACGACGCCGTTTTGGTCGAAACGGAAGAAAGGGCTCGGCTTTGTGATTCTGGTGCTGCTGTGGGGTTAGTTCAAGTTGGGTTTTTGGAGAGATTGGGTGATTCTAAATTTGAGTTTAGCTACAATGTGAGGGTTATGAAGTGTTTGAGTGGAATGAGAGAAGAGGAGAGGAGAGAATTGGGTTTGTTGCTGAGAGGTTCTGGGAGGCAGTGTAGGAGGATAGGGAAGGTTTATGGGTTTTGGGGAAATGTGGAAGATGGGGTTTTGTATTTAGTGTGTGAGAGCCAGAGTGGGAGGTTTGAGGAGAAGTTGAGGGGTTTGAGAAATGGGGATGGGTTTGGGAGAGATGGGTTGGGTGGTTTTGGTGTGATTGGTATGGAGGTGTGTGAGGCGGTGATGGGGTTGCATTCGGAGGGGTTTGTTGGTGGTGGTTTTGGTGTTTCGTGTTTTGGTTTCGATGATTTTGGTCATGTTTTTGTGGATTTGAATGAGGTGTTGGTGATGGGAAGGAAGGTTTGGACAAGTGTTGGTGGTAGAAAGGGAATTGGTGCTGAAGAAATGGGAGTGATGTTTGGCAGTTTATTGAAAGATGAGGCTTTTGTTAGCATGGAGGTGTTGTTTGAGGTGTTGAAGAAAGAGGGCATTGCAGTGGAATGTGAGAGCTCGAAATATATGGTTGGGTATGACTCGGATGTTTGGTCATTAGCTTGTGTTTTGCTCAGTCTGCTTCTTGGGAAAGAGTTCAGTGAAGAGATTGGAAATATGAATCATATTTGCGATCATTCAACGTATGCAAGTTGGATTGAAAGAGTTGGTGATTTGCTGGACAGTAGATTGGGTTCTGAATATGCATCACTGAGGGTGACTCTCTGTAAATGTTTGAATTATGATCCAGCAAGTCGTCCACTAGTGATTGATGTTATGATTTGCATTAGGGAACTGATTATTAAACCTCAATATGATATCATGGCCGGTTTGGAGAGACCGGTTAAGGAGAACAGCACAAGTTGTTGCTTGATTCTGGGGAGCTCTGTCAGATGCCCAAGAAAATGTCAGAAACACAGAAAGAACATGGGTTGCAGGGAAAAGAAGTCTGAGAATGGTTTTGTTGATGGACTCGCAGAGGGAAAGGTCAAATCTAAAGTTCTGCAGGGCCATCGTGACTCTATTACAGCATTAGCTGTTGGAGGAGAATTTCTATTCAGCTCCTCATTTGACAAAACCATCCATGTGTGGTCTTTGCAGGACTTCTGTCATGTACATACATTTAAAGGCCATGAGCATACCATTAAGGCTCTAATTTATGTAGATGAAGAGAAACCCTTGTGCATAAGTGGTGACAGTGGAGGTGGTATATTTGTCTGGAGCACAAGTTCCCCTCTAGGGCAAGAACCATTGAAGATACTGTATGAGCAGAAAGATTGGCGCTTTAGTGGTATTCATGCCTTGGCCTTTAGAGATGGATATATTTATACCGGCAGTGGAGACAGAACAGTAAAAGCATGGTCTATGCAGGACGGCACCATATCATGCACTATGTCTGGTCATAAATCAGTAGTTTCAACACTTACAGTTTGTGATAGTGTTCTGTATAGCGGCAGTTGGGATGGAACCATACGATTATGGAGTCTCAGCGATCACAGTCCTTTGGCAGTACTAGGGGAAGACACATCTGGAACTGTGGCTTCTGTCTTATCTCTTGCTGCTGACAGACATGTGCTCATTGCAACCCATGAAAACGGATGTCTAAAGGTCTGGAAAAATGATGTGTTTATGAAATCCATTAAAATGCACAATGGTGCAGTTTTTGCTACTGGCCTGGATGGAAAATGGCTTTTCACAGGCGGGTTGGATAAAACTGTCAATGTCCAGGAATGGTCTGGTGATGAGTTTCAAACAGATTTCAGACCTATTGGATCCATCCCCTGTGATTCTGTCATTACAACTTTGTTGTGCTGGCAAGGAAAGCTTTTCGTTGGATATGCTAATTGGAATATCATGGTGTTTTACTATGGTAAATGA

Protein Analysis

798

Amino Acids

88.21

Weight (kDa)

5.71

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_UBOX PF13445 6 - 48 6.4e-07 RING-type zinc-finger
WDR55 PF24796 498 - 712 5.5e-13 WDR55
Beta-prop_SCAP PF24017 505 - 581 2e-06 SCAP Beta-propeller
WD40_Gbeta PF25391 505 - 667 3.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 507 - 580 1.6e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 508 - 726 6.4e-24 WDR5 beta-propeller domain
WD40_Prp19 PF24814 603 - 748 6.9e-09 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 612 - 727 8.7e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 614 - 748 9.3e-14 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 614 - 710 3.2e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 614 - 675 9.9e-08 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015005)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21651
fragaria_vesca FvH4_2g27740
prunus_persica Prupe.1G490500_v2.0.a1 Prupe.1G490500_v2.0.a1 Prupe.1G490500_v2.0.a1
pyrus_communis pycom15g30580
rosa_chinensis RchiOBHm_Chr6g0296591
rosa_laevigata RLG00000011677
rosa_multiflora Rmu_sc0001741.1_g000020
rosa_roxburghii Rroxscaffold_6G00390100 Rroxscaffold_7G00178750
rosa_rugosa Rorug06G0263600
rosa_samantha Rh6BG383500 Rh6CG388600 Rh6DG376000
rosa_wichuraiana Rw6G032710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 179, 1895
AccI GTMKAC 1 cut(s) 50
AccII CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
AclWI GGATC 4 cut(s) 260, 1259, 2286, 2299
AcoI YGGCCR 2 cut(s) 76, 1344
AcsI RAATTY 2 cut(s) 482, 1568
AcuI CTGAAG 1 cut(s) 948
AcyI GRCGYC 2 cut(s) 180, 381
AfaI GTAC 5 cut(s) 151, 167, 377, 1635, 2031
AfiI CCNNNNNNNGG 3 cut(s) 124, 176, 1758
AflIII ACRYGT 1 cut(s) 2089
AgeI ACCGGT 1 cut(s) 1360
AhdI GACNNNNNGTC 1 cut(s) 46
AhlI ACTAGT 1 cut(s) 1282
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 3 cut(s) 99, 2193, 2244
AjuI GAANNNNNNNTTGG 4 cut(s) 854, 874, 886, 906
AleI CACNNNNGTG 1 cut(s) 2172
AloI GAACNNNNNNTCC 2 cut(s) 1732, 1764
Alw21I GWGCWC 4 cut(s) 1040, 1406, 1745, 2097
Alw26I GTCTC 4 cut(s) 122, 1351, 1854, 2009
AlwI GGATC 4 cut(s) 260, 1259, 2286, 2299
AoxI GGCC 6 cut(s) 76, 1344, 1528, 1648, 1824, 2191
ApeKI GCWGC 5 cut(s) 109, 138, 190, 431, 2078
ApoI RAATTY 2 cut(s) 482, 1568
AsiGI ACCGGT 1 cut(s) 1360
Asp700I GAANNNNTTC 1 cut(s) 1115
AspLEI GCGC 5 cut(s) 47, 63, 182, 190, 1803
AspS9I GGNCC 2 cut(s) 333, 1528
AsuC2I CCSGG 4 cut(s) 20, 125, 143, 271
AsuHPI GGTGA 7 cut(s) 485, 781, 886, 1202, 1249, 1724, 2270
AvaII GGWCC 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 2291
BanI GGYRCC 2 cut(s) 179, 1895
BanII GRGCYC 3 cut(s) 412, 1040, 1406
BbsI GAAGAC 1 cut(s) 2046
Bbv12I GWGCWC 4 cut(s) 1040, 1406, 1745, 2097
BbvI GCAGC 5 cut(s) 96, 125, 177, 418, 2065
BceAI ACGGC 4 cut(s) 58, 193, 368, 1909
BcgI CGANNNNNNTGC 2 cut(s) 1514, 1548
BciT130I CCWGG 3 cut(s) 101, 2195, 2246
BcnI CCSGG 4 cut(s) 20, 125, 143, 271
BcoDI GTCTC 4 cut(s) 122, 1351, 1854, 2009
BcuI ACTAGT 1 cut(s) 1282
BfaI CTAG 3 cut(s) 1283, 1757, 2033
BfmI CTRYAG 2 cut(s) 29, 1523
BfoI RGCGCY 2 cut(s) 183, 1804
BmcAI AGTACT 1 cut(s) 2031
Bme1390I CCNGG 7 cut(s) 20, 101, 125, 143, 271, 2195, 2246
Bme18I GGWCC 1 cut(s) 333
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 333, 1528
BmiI GGNNCC 4 cut(s) 181, 1897, 1987, 2293
BmrFI CCNGG 7 cut(s) 20, 101, 125, 143, 271, 2195, 2246
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 2 cut(s) 1235, 1403
BmuI ACTGGG 1 cut(s) 373
BoxI GACNNNNGTC 1 cut(s) 1626
BpiI GAAGAC 1 cut(s) 2046
BplI GAGNNNNNCTC 2 cut(s) 1225, 1257
BpmI CTGGAG 1 cut(s) 1759
BpuMI CCSGG 4 cut(s) 20, 125, 143, 271
BsaHI GRCGYC 2 cut(s) 180, 381
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 4 cut(s) 269, 291, 323, 1821
BsaWI WCCGGW 2 cut(s) 10, 1360
BsaXI ACNNNNNCTCC 6 cut(s) 571, 601, 977, 1007, 1732, 1762
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 176, 1758
Bse118I RCCGGY 3 cut(s) 1346, 1360, 1850
Bse1I ACTGG 2 cut(s) 368, 2194
Bse3DI GCAATG 2 cut(s) 1017, 2097
BseBI CCWGG 3 cut(s) 101, 2195, 2246
BseDI CCNNGG 4 cut(s) 269, 291, 323, 1821
BseGI GGATG 8 cut(s) 301, 712, 1072, 1599, 1987, 2123, 2203, 2295
BseLI CCNNNNNNNGG 3 cut(s) 124, 176, 1758
BseMI GCAATG 2 cut(s) 1017, 2097
BseMII CTCAG 7 cut(s) 167, 185, 557, 1108, 1223, 1461, 2020
BseNI ACTGG 2 cut(s) 368, 2194
BseRI GAGGAG 5 cut(s) 554, 559, 692, 1573, 1578
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 5 cut(s) 96, 125, 177, 418, 2065
BseYI CCCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 2196
Bsh1236I CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
BshFI GGCC 6 cut(s) 78, 1346, 1530, 1650, 1826, 2193
BshNI GGYRCC 2 cut(s) 179, 1895
BshTI ACCGGT 1 cut(s) 1360
BsiHKAI GWGCWC 4 cut(s) 1040, 1406, 1745, 2097
BsiSI CCGG 8 cut(s) 11, 20, 125, 143, 271, 1347, 1361, 1851
BslI CCNNNNNNNGG 3 cut(s) 124, 176, 1758
BsmAI GTCTC 4 cut(s) 122, 1351, 1854, 2009
BsmBI CGTCTC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 6 cut(s) 78, 1346, 1530, 1650, 1826, 2193
Bso31I GGTCTC 1 cut(s) 1351
Bsp1286I GDGCHC 6 cut(s) 20, 412, 1040, 1406, 1745, 2097
Bsp1407I TGTACA 1 cut(s) 1633
Bsp143I GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
BspANI GGCC 6 cut(s) 78, 1346, 1530, 1650, 1826, 2193
BspCNI CTCAG 7 cut(s) 166, 184, 558, 1107, 1224, 1462, 2019
BspFNI CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
BspLI GGNNCC 4 cut(s) 181, 1897, 1987, 2293
BspMAI CTGCAG 2 cut(s) 33, 1527
BspPI GGATC 4 cut(s) 260, 1259, 2286, 2299
BspT107I GGYRCC 2 cut(s) 179, 1895
BspTNI GGTCTC 1 cut(s) 1351
BsrDI GCAATG 2 cut(s) 1017, 2097
BsrFI RCCGGY 3 cut(s) 1346, 1360, 1850
BsrGI TGTACA 1 cut(s) 1633
BsrI ACTGG 2 cut(s) 368, 2194
BssAI RCCGGY 3 cut(s) 1346, 1360, 1850
BssECI CCNNGG 4 cut(s) 269, 291, 323, 1821
BssMI GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
BssNI GRCGYC 2 cut(s) 180, 381
BssT1I CCWWGG 1 cut(s) 1821
Bst2UI CCWGG 3 cut(s) 101, 2195, 2246
Bst4CI ACNGT 8 cut(s) 1205, 1718, 1782, 1870, 1948, 2018, 2056, 2236
Bst6I CTCTTC 3 cut(s) 531, 1119, 1683
BstACI GRCGYC 2 cut(s) 180, 381
BstAPI GCANNNNNTGC 1 cut(s) 2183
BstAUI TGTACA 1 cut(s) 1633
BstC8I GCNNGC 4 cut(s) 65, 88, 219, 2333
BstDEI CTNAG 7 cut(s) 153, 171, 566, 1094, 1232, 1470, 2006
BstDSI CCRYGG 2 cut(s) 291, 323
BstF5I GGATG 8 cut(s) 301, 712, 1072, 1599, 1987, 2123, 2203, 2295
BstFNI CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
BstH2I RGCGCY 2 cut(s) 183, 1804
BstHHI GCGC 5 cut(s) 47, 63, 182, 190, 1803
BstKTI GATC 5 cut(s) 268, 1156, 1267, 2014, 2294
BstMAI GTCTC 4 cut(s) 122, 1351, 1854, 2009
BstMBI GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
BstMWI GCNNNNNNNGC 5 cut(s) 96, 283, 1099, 2183, 2341
BstNI CCWGG 3 cut(s) 101, 2195, 2246
BstNSI RCATGY 1 cut(s) 2093
BstPAI GACNNNNGTC 1 cut(s) 1626
BstSCI CCNGG 7 cut(s) 18, 99, 123, 141, 269, 2193, 2244
BstSFI CTRYAG 2 cut(s) 29, 1523
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
BstV1I GCAGC 5 cut(s) 96, 125, 177, 418, 2065
BstV2I GAAGAC 1 cut(s) 2046
BstX2I RGATCY 1 cut(s) 2291
BstYI RGATCY 1 cut(s) 2291
BsuRI GGCC 6 cut(s) 78, 1346, 1530, 1650, 1826, 2193
BtgI CCRYGG 2 cut(s) 291, 323
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 8 cut(s) 301, 712, 1072, 1599, 1987, 2123, 2203, 2295
BtsI GCAGTG 3 cut(s) 590, 1029, 1861
BtsIMutI CAGTG 6 cut(s) 590, 1029, 1126, 1229, 1723, 1861
Cac8I GCNNGC 4 cut(s) 65, 88, 219, 2333
CfoI GCGC 5 cut(s) 47, 63, 182, 190, 1803
Cfr10I RCCGGY 3 cut(s) 1346, 1360, 1850
Cfr13I GGNCC 2 cut(s) 333, 1528
Cfr42I CCGCGG 2 cut(s) 294, 326
CseI GACGC 2 cut(s) 36, 389
Csp6I GTAC 5 cut(s) 150, 166, 376, 1634, 2030
CspAI ACCGGT 1 cut(s) 1360
CviQI GTAC 5 cut(s) 150, 166, 376, 1634, 2030
DdeI CTNAG 7 cut(s) 153, 171, 566, 1094, 1232, 1470, 2006
DinI GGCGCC 1 cut(s) 181
DpnI GATC 5 cut(s) 267, 1155, 1266, 2013, 2293
DpnII GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
DraI TTTAAA 1 cut(s) 1645
DriI GACNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 76, 1344
Eam1104I CTCTTC 3 cut(s) 531, 1119, 1683
Eam1105I GACNNNNNGTC 1 cut(s) 46
EarI CTCTTC 3 cut(s) 531, 1119, 1683
EciI GGCGGA 1 cut(s) 225
Ecl136II GAGCTC 2 cut(s) 1038, 1404
Eco130I CCWWGG 1 cut(s) 1821
Eco24I GRGCYC 3 cut(s) 412, 1040, 1406
Eco31I GGTCTC 1 cut(s) 1351
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 2 cut(s) 1038, 1404
Eco57I CTGAAG 1 cut(s) 948
EcoICRI GAGCTC 2 cut(s) 1038, 1404
EcoRII CCWGG 3 cut(s) 99, 2193, 2244
EcoRV GATATC 1 cut(s) 1339
EcoT14I CCWWGG 1 cut(s) 1821
EcoT22I ATGCAT 1 cut(s) 1228
EcoT38I GRGCYC 3 cut(s) 412, 1040, 1406
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 1821
Esp3I CGTCTC 1 cut(s) 122
FalI AAGNNNNNCTT 2 cut(s) 2328, 2360
FauI CCCGC 1 cut(s) 2213
FblI GTMKAC 1 cut(s) 50
FokI GGATG 8 cut(s) 308, 719, 1079, 1586, 1994, 2130, 2210, 2282
FriOI GRGCYC 3 cut(s) 412, 1040, 1406
FspBI CTAG 3 cut(s) 1283, 1757, 2033
GlaI GCGC 5 cut(s) 46, 62, 181, 189, 1802
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 1 cut(s) 1759
HaeII RGCGCY 2 cut(s) 183, 1804
HaeIII GGCC 6 cut(s) 78, 1346, 1530, 1650, 1826, 2193
HapII CCGG 8 cut(s) 11, 20, 125, 143, 271, 1347, 1361, 1851
HgaI GACGC 2 cut(s) 36, 389
HhaI GCGC 5 cut(s) 47, 63, 182, 190, 1803
Hin1I GRCGYC 2 cut(s) 180, 381
Hin6I GCGC 5 cut(s) 45, 61, 180, 188, 1801
HinP1I GCGC 5 cut(s) 45, 61, 180, 188, 1801
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 2342
HpaII CCGG 8 cut(s) 11, 20, 125, 143, 271, 1347, 1361, 1851
HphI GGTGA 7 cut(s) 485, 781, 886, 1202, 1249, 1724, 2270
Hpy166II GTNNAC 3 cut(s) 51, 150, 1280
Hpy188III TCNNGA 4 cut(s) 1535, 1738, 2049, 2131
Hpy8I GTNNAC 3 cut(s) 51, 150, 1280
Hpy99I CGWCG 6 cut(s) 44, 52, 55, 266, 341, 383
HpyAV CCTTC 5 cut(s) 218, 352, 595, 876, 880
HpyCH4III ACNGT 8 cut(s) 1205, 1718, 1782, 1870, 1948, 2018, 2056, 2236
HpyCH4IV ACGT 2 cut(s) 280, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 283, 1099, 2183, 2341
HpyF3I CTNAG 7 cut(s) 153, 171, 566, 1094, 1232, 1470, 2006
HpySE526I ACGT 2 cut(s) 280, 1163
Hsp92I GRCGYC 2 cut(s) 180, 381
HspAI GCGC 5 cut(s) 45, 61, 180, 188, 1801
KasI GGCGCC 1 cut(s) 179
KspI CCGCGG 2 cut(s) 294, 326
Kzo9I GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
LmnI GCTCC 4 cut(s) 4, 1401, 1586, 1740
Lsp1109I GCAGC 5 cut(s) 96, 125, 177, 418, 2065
LweI GCATC 2 cut(s) 1235, 1403
MaeI CTAG 3 cut(s) 1283, 1757, 2033
MaeII ACGT 2 cut(s) 280, 1163
MaeIII GTNAC 3 cut(s) 1237, 1535, 1712
MalI GATC 5 cut(s) 267, 1155, 1266, 2013, 2293
MboI GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
MboII GAAGA 9 cut(s) 413, 548, 641, 941, 1018, 1136, 1700, 1786, 2051
MflI RGATCY 1 cut(s) 2291
MhlI GDGCHC 6 cut(s) 20, 412, 1040, 1406, 1745, 2097
MluCI AATT 9 cut(s) 256, 313, 482, 551, 918, 1258, 1568, 1674, 2362
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 5 cut(s) 1055, 1234, 1485, 1532, 2011
MmeI TCCRAC 6 cut(s) 285, 360, 1154, 1540, 2205, 2332
Mph1103I ATGCAT 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 1115
MseI TTAA 6 cut(s) 312, 1323, 1365, 1644, 1665, 2160
MslI CAYNNNNRTG 5 cut(s) 986, 1022, 1604, 2162, 2172
MspA1I CMGCKG 2 cut(s) 293, 325
MspI CCGG 8 cut(s) 11, 20, 125, 143, 271, 1347, 1361, 1851
MspR9I CCNGG 7 cut(s) 20, 101, 125, 143, 271, 2195, 2246
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 101, 2195, 2246
MvnI CGCG 6 cut(s) 47, 61, 63, 188, 293, 325
MwoI GCNNNNNNNGC 5 cut(s) 96, 283, 1099, 2183, 2341
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 4 cut(s) 20, 125, 143, 271
NdeII GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
NlaIV GGNNCC 4 cut(s) 181, 1897, 1987, 2293
NmeAIII GCCGAG 2 cut(s) 62, 391
NmuCI GTSAC 3 cut(s) 1237, 1535, 1712
NsiI ATGCAT 1 cut(s) 1228
NspI RCATGY 1 cut(s) 2093
OliI CACNNNNGTG 1 cut(s) 2172
PciI ACATGT 1 cut(s) 2089
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 1115
PfeI GAWTC 7 cut(s) 55, 133, 422, 476, 1141, 1393, 2306
PfoI TCCNGGA 1 cut(s) 2244
PinAI ACCGGT 1 cut(s) 1360
PleI GAGTC 5 cut(s) 1055, 1234, 1485, 1532, 2010
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 5 cut(s) 1055, 1234, 1485, 1532, 2010
PscI ACATGT 1 cut(s) 2089
PshAI GACNNNNGTC 1 cut(s) 1626
Psp124BI GAGCTC 2 cut(s) 1040, 1406
Psp6I CCWGG 3 cut(s) 99, 2193, 2244
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 3 cut(s) 99, 2193, 2244
PspN4I GGNNCC 4 cut(s) 181, 1897, 1987, 2293
PspPI GGNCC 2 cut(s) 333, 1528
PsrI GAACNNNNNNTAC 2 cut(s) 1198, 1230
PstI CTGCAG 2 cut(s) 33, 1527
PsuI RGATCY 1 cut(s) 2291
RsaI GTAC 5 cut(s) 151, 167, 377, 1635, 2031
RsaNI GTAC 5 cut(s) 150, 166, 376, 1634, 2030
RseI CAYNNNNRTG 5 cut(s) 986, 1022, 1604, 2162, 2172
SacI GAGCTC 2 cut(s) 1040, 1406
SacII CCGCGG 2 cut(s) 294, 326
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 6 cut(s) 312, 1323, 1365, 1644, 1665, 2160
Sau3AI GATC 5 cut(s) 265, 1153, 1264, 2011, 2291
Sau96I GGNCC 2 cut(s) 333, 1528
ScaI AGTACT 1 cut(s) 2031
SchI GAGTC 5 cut(s) 1055, 1234, 1485, 1532, 2011
ScrFI CCNGG 7 cut(s) 20, 101, 125, 143, 271, 2195, 2246
SduI GDGCHC 6 cut(s) 20, 412, 1040, 1406, 1745, 2097
SfaNI GCATC 2 cut(s) 1235, 1403
SfcI CTRYAG 2 cut(s) 29, 1523
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 2 cut(s) 294, 326
SgrBI CCGCGG 2 cut(s) 294, 326
SgrDI CGTCGACG 1 cut(s) 49
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 5 cut(s) 986, 1022, 1604, 2162, 2172
SpeI ACTAGT 1 cut(s) 1282
Sse9I AATT 9 cut(s) 256, 313, 482, 551, 918, 1258, 1568, 1674, 2362
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 3 cut(s) 1283, 1757, 2033
SstI GAGCTC 2 cut(s) 1040, 1406
StyD4I CCNGG 7 cut(s) 18, 99, 123, 141, 269, 2193, 2244
StyI CCWWGG 1 cut(s) 1821
TaaI ACNGT 8 cut(s) 1205, 1718, 1782, 1870, 1948, 2018, 2056, 2236
TaiI ACGT 2 cut(s) 283, 1166
TaqI TCGA 5 cut(s) 50, 204, 393, 831, 1040
TasI AATT 9 cut(s) 256, 313, 482, 551, 918, 1258, 1568, 1674, 2362
TatI WGTACW 3 cut(s) 149, 1633, 2029
TauI GCSGC 7 cut(s) 78, 185, 188, 241, 325, 328, 1974
TfiI GAWTC 7 cut(s) 55, 133, 422, 476, 1141, 1393, 2306
Tru1I TTAA 6 cut(s) 312, 1323, 1365, 1644, 1665, 2160
Tru9I TTAA 6 cut(s) 312, 1323, 1365, 1644, 1665, 2160
TscAI CASTG 6 cut(s) 590, 1029, 1126, 1236, 1723, 1861
TseFI GTSAC 3 cut(s) 1237, 1535, 1712
TseI GCWGC 5 cut(s) 109, 138, 190, 431, 2078
Tsp45I GTSAC 3 cut(s) 1237, 1535, 1712
TspDTI ATGAA 6 cut(s) 527, 1154, 1701, 1805, 2124, 2165
TspGWI ACGGA 2 cut(s) 413, 2130
TspRI CASTG 6 cut(s) 590, 1029, 1126, 1236, 1723, 1861
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 2 cut(s) 482, 1568
XceI RCATGY 1 cut(s) 2093
XcmI CCANNNNNNNNNTGG 1 cut(s) 2252
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 1115
XspI CTAG 3 cut(s) 1283, 1757, 2033
ZrmI AGTACT 1 cut(s) 2031
Zsp2I ATGCAT 1 cut(s) 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.