pycom15g33360

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015832
Physical Location & Seq
Forward (+)
32798374 .. 32801759
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGGGAAAGGCGTTTCTTCTTCAAGGTGGGGACTGCGCTGAGAGTTTCAAGGAGTTCAATGCCAATAACATTCGTGACACCTTCAGAATTCTCCTCCAAATGGGTGTCGTTTTGATGTTCGGAGGCCAAATGCCCGTCGTCAAGGTGGGAAGAATGGCGGGTCAGTTTGCGAAACCCAGATCGGATTCTTTCGAGGAGAAGAATGGTGTGAAGCTGCCTAGTTATAGAGGAGACAATGTGAATGGAGACGCCTTTGATTTACAGTCGAGGACTCCAGACCCTCAGAGACTGATCAGAGCCTATTGCCAATCCGCTGCTACTCTAAACCTTCTGAGGGCGTTCGCCACCGGAGGTTACGCAGCTATGCAGAGGGTCACACAGTGGAACTTGGATTTCACAGAGCACAGCGAGCAGGGTGATAGATATCGGGAGCTAGCTGCCCGGGTGGATGAGGCCCTTGGATTCATGACTGCTGCCGGGCTCACAGTTGAACACCCTATCATGACAACCACTGAGTTCTGGACGTCTCATGAGTGCTTGCTCTTGCCTTACGAGCAGTCTCTTACTAGGTTGGATTCAACTTCCGGCCTTTACTATGATTGCTCTGCCCATATGCTTTGGGCTGGTGAGCGTACCAGGCAACTGGATGGTGCTCATGTTGAGTTCCTTAGAGGTGTTGCCAATCCCCTTGGAATCAAGGTCAGCGACAAGATGGATCCAAACGAGCTTGTTAAGCTCATTGAAATTTTGAATCCTCAGAATAAAGCTGGGAGGATAACAATCATCACAAGAATGGGAGCTGAGAACATGAGGGTGAAACTTCCTCATCTTATTAGGGCAGTCCGCAGGGCAGGGCAGATTGTCACATGGGTTAGTGACCCAATGCATGGAAACACCATTAAGGCTCCTTGTGGTCTCAAAACACGTCCTTTCGATGCCATAAGGGCTGAGGTGAGAGCGTTCTTTGATGTGCACGAGCAAGAAGGAAGCCATCCAGGTGGTGTTCATCTAGAGATGACAGGCCAGAATGTGACAGAATGCATCGGAGGTTCACGCACTGTGACATTTGACGACCTTAGCTCGCGCTACCACACTCACTGCGACCCAAGGCTCAATGCCTCGCAATCTCTTGAGCTGGCCTTCATCATCGCCGAGCGCCTCAGGAAAAGAAGGATCAAGTCACAGCATTCAGTTGCGGCCTTGGATCTGTAG

Protein Analysis

404

Amino Acids

45.04

Weight (kDa)

8.14

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 2 - 384 9.7e-186 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22410
fragaria_vesca FvH4_5g36810
malus_domestica MD08G1186700.v1.1 MD15G1372600.v1.1
prunus_persica Prupe.1G521200_v2.0.a1
pyrus_communis pycom08g16050 pycom15g33360
rosa_chinensis RchiOBHm_Chr7g0243371
rosa_laevigata RLG00000000852
rosa_multiflora Rmu_sc0001036.1_g000028 Rmu_sc0001461.1_g000106
rosa_roxburghii Rroxscaffold_3G00222150
rosa_rugosa Rorug07G0318600
rosa_samantha Rh7BG444800
rosa_wichuraiana Rw7G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 527
AccB7I CCANNNNNTGG 1 cut(s) 887
AccII CGCG 1 cut(s) 1084
AciI CCGC 4 cut(s) 158, 312, 844, 1196
AclWI GGATC 3 cut(s) 710, 723, 1181
AcsI RAATTY 2 cut(s) 87, 744
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 2 cut(s) 249, 524
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 1 cut(s) 634
AfiI CCNNNNNNNGG 3 cut(s) 100, 334, 887
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 7 cut(s) 23, 49, 58, 491, 579, 743, 751
AjiI CACGTC 1 cut(s) 926
AjnI CCWGG 2 cut(s) 635, 994
Alw21I GWGCWC 3 cut(s) 405, 655, 975
Alw26I GTCTC 6 cut(s) 225, 240, 280, 531, 564, 920
Alw44I GTGCAC 1 cut(s) 971
AlwI GGATC 3 cut(s) 710, 723, 1181
AlwNI CAGNNNCTG 1 cut(s) 289
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 6 cut(s) 124, 453, 586, 1021, 1137, 1197
ApaLI GTGCAC 1 cut(s) 971
ApeKI GCWGC 5 cut(s) 214, 314, 359, 437, 473
ApoI RAATTY 2 cut(s) 87, 744
AspLEI GCGC 3 cut(s) 38, 1086, 1158
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 3 cut(s) 442, 443, 478
AsuHPI GGTGA 4 cut(s) 428, 638, 826, 964
AsuNHI GCTAGC 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 441
AxyI CCTNAGG 1 cut(s) 1160
BaeGI GKGCMC 1 cut(s) 975
BamHI GGATCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 483
BauI CACGAG 1 cut(s) 974
Bbv12I GWGCWC 3 cut(s) 405, 655, 975
BbvCI CCTCAGC 1 cut(s) 948
BbvI GCAGC 5 cut(s) 201, 301, 371, 424, 460
BccI CCATC 3 cut(s) 641, 706, 999
BciT130I CCWGG 2 cut(s) 637, 996
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 3 cut(s) 442, 443, 478
BcoDI GTCTC 6 cut(s) 225, 240, 280, 531, 564, 920
BfaI CTAG 4 cut(s) 219, 434, 567, 1010
BfmI CTRYAG 1 cut(s) 1208
BfoI RGCGCY 1 cut(s) 1159
BglI GCCNNNNNGGC 1 cut(s) 944
BisI GCNGC 6 cut(s) 215, 315, 360, 438, 474, 1197
BlsI GCNGC 6 cut(s) 216, 316, 361, 439, 475, 1198
Bme1390I CCNGG 5 cut(s) 442, 443, 478, 637, 996
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 717, 906
BmrFI CCNGG 5 cut(s) 442, 443, 478, 637, 996
BmsI GCATC 2 cut(s) 925, 1050
BmtI GCTAGC 1 cut(s) 437
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 2 cut(s) 948, 1076
BpuEI CTTGAG 1 cut(s) 1151
BpuMI CCSGG 3 cut(s) 442, 443, 478
BsaBI GATNNNNATC 2 cut(s) 423, 779
BsaHI GRCGYC 2 cut(s) 249, 524
BsaI GGTCTC 1 cut(s) 920
BsaJI CCNNGG 5 cut(s) 441, 457, 688, 1106, 1200
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 334, 887
Bse1I ACTGG 1 cut(s) 648
Bse21I CCTNAGG 1 cut(s) 1160
Bse8I GATNNNNATC 2 cut(s) 423, 779
BseBI CCWGG 2 cut(s) 637, 996
BseDI CCNNGG 5 cut(s) 441, 457, 688, 1106, 1200
BseGI GGATG 3 cut(s) 454, 652, 991
BseJI GATNNNNATC 2 cut(s) 423, 779
BseLI CCNNNNNNNGG 3 cut(s) 100, 334, 887
BseMII CTCAG 8 cut(s) 30, 296, 323, 504, 770, 792, 939, 1174
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 3 cut(s) 83, 209, 243
BseSI GKGCMC 1 cut(s) 975
BseXI GCAGC 5 cut(s) 201, 301, 371, 424, 460
BseYI CCCAGC 1 cut(s) 767
Bsh1236I CGCG 1 cut(s) 1084
BshFI GGCC 6 cut(s) 126, 455, 588, 1023, 1139, 1199
BsiHKAI GWGCWC 3 cut(s) 405, 655, 975
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 4 cut(s) 348, 442, 477, 585
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 3 cut(s) 100, 334, 887
BsmAI GTCTC 6 cut(s) 225, 240, 280, 531, 564, 920
BsmBI CGTCTC 2 cut(s) 240, 531
BsmFI GGGAC 1 cut(s) 44
BsmI GAATGC 2 cut(s) 1043, 1186
BsnI GGCC 6 cut(s) 126, 455, 588, 1023, 1139, 1199
Bso31I GGTCTC 1 cut(s) 920
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 4 cut(s) 405, 483, 655, 975
Bsp143I GATC 5 cut(s) 179, 291, 715, 1173, 1204
BspACI CCGC 4 cut(s) 158, 312, 844, 1196
BspANI GGCC 6 cut(s) 126, 455, 588, 1023, 1139, 1199
BspCNI CTCAG 8 cut(s) 31, 295, 324, 505, 769, 793, 940, 1173
BspFNI CGCG 1 cut(s) 1084
BspHI TCATGA 3 cut(s) 465, 501, 529
BspLI GGNNCC 2 cut(s) 717, 906
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 3 cut(s) 710, 723, 1181
BspTNI GGTCTC 1 cut(s) 920
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 5 cut(s) 441, 457, 688, 1106, 1200
BssMI GATC 5 cut(s) 179, 291, 715, 1173, 1204
BssNI GRCGYC 2 cut(s) 249, 524
BssSI CACGAG 1 cut(s) 974
BssT1I CCWWGG 4 cut(s) 457, 688, 1106, 1200
Bst2BI CACGAG 1 cut(s) 974
Bst2UI CCWGG 2 cut(s) 637, 996
Bst4CI ACNGT 4 cut(s) 264, 381, 487, 1060
BstACI GRCGYC 2 cut(s) 249, 524
BstC8I GCNNGC 5 cut(s) 410, 435, 539, 1082, 1137
BstF5I GGATG 3 cut(s) 454, 652, 991
BstFNI CGCG 1 cut(s) 1084
BstH2I RGCGCY 1 cut(s) 1159
BstHHI GCGC 3 cut(s) 38, 1086, 1158
BstKTI GATC 5 cut(s) 182, 294, 718, 1176, 1207
BstMAI GTCTC 6 cut(s) 225, 240, 280, 531, 564, 920
BstMBI GATC 5 cut(s) 179, 291, 715, 1173, 1204
BstMWI GCNNNNNNNGC 5 cut(s) 409, 553, 637, 733, 944
BstNI CCWGG 2 cut(s) 637, 996
BstSCI CCNGG 5 cut(s) 440, 441, 476, 635, 994
BstSFI CTRYAG 1 cut(s) 1208
BstSLI GKGCMC 1 cut(s) 975
BstUI CGCG 1 cut(s) 1084
BstV1I GCAGC 5 cut(s) 201, 301, 371, 424, 460
BstX2I RGATCY 2 cut(s) 715, 1204
BstXI CCANNNNNNTGG 2 cut(s) 643, 998
BstYI RGATCY 2 cut(s) 715, 1204
Bsu36I CCTNAGG 1 cut(s) 1160
BsuRI GGCC 6 cut(s) 126, 455, 588, 1023, 1139, 1199
BtgZI GCGATG 1 cut(s) 1132
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 3 cut(s) 454, 652, 991
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 4 cut(s) 386, 510, 1056, 1096
Cac8I GCNNGC 5 cut(s) 410, 435, 539, 1082, 1137
CaiI CAGNNNCTG 1 cut(s) 289
CciI TCATGA 3 cut(s) 465, 501, 529
CfoI GCGC 3 cut(s) 38, 1086, 1158
Cfr13I GGNCC 1 cut(s) 454
Cfr9I CCCGGG 1 cut(s) 441
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 633
CviAII CATG 7 cut(s) 466, 502, 530, 656, 808, 867, 887
CviQI GTAC 1 cut(s) 633
DpnI GATC 5 cut(s) 181, 293, 717, 1175, 1206
DpnII GATC 5 cut(s) 179, 291, 715, 1173, 1204
DraIII CACNNNGTG 1 cut(s) 381
Eco130I CCWWGG 4 cut(s) 457, 688, 1106, 1200
Eco24I GRGCYC 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 920
Eco32I GATATC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 67
Eco81I CCTNAGG 1 cut(s) 1160
Eco88I CYCGRG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 635, 994
EcoRV GATATC 1 cut(s) 425
EcoT14I CCWWGG 4 cut(s) 457, 688, 1106, 1200
EcoT22I ATGCAT 2 cut(s) 888, 1043
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 4 cut(s) 457, 688, 1106, 1200
Esp3I CGTCTC 2 cut(s) 240, 531
FaeI CATG 7 cut(s) 469, 505, 533, 659, 811, 870, 890
FaqI GGGAC 1 cut(s) 44
FatI CATG 7 cut(s) 465, 501, 529, 655, 807, 866, 886
FauI CCCGC 1 cut(s) 151
FauNDI CATATG 1 cut(s) 612
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 6 cut(s) 215, 315, 360, 438, 474, 1197
FokI GGATG 3 cut(s) 461, 659, 978
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 6 cut(s) 215, 315, 360, 438, 474, 1197
FspBI CTAG 4 cut(s) 219, 434, 567, 1010
GlaI GCGC 3 cut(s) 37, 1085, 1157
GluI GCNGC 6 cut(s) 215, 315, 360, 438, 474, 1197
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 1159
HaeIII GGCC 6 cut(s) 126, 455, 588, 1023, 1139, 1199
HapII CCGG 4 cut(s) 348, 442, 477, 585
HgaI GACGC 1 cut(s) 257
HhaI GCGC 3 cut(s) 38, 1086, 1158
Hin1I GRCGYC 2 cut(s) 249, 524
Hin1II CATG 7 cut(s) 469, 505, 533, 659, 811, 870, 890
Hin6I GCGC 3 cut(s) 36, 1084, 1156
HinP1I GCGC 3 cut(s) 36, 1084, 1156
HinfI GANTC 6 cut(s) 185, 271, 462, 575, 693, 751
HpaII CCGG 4 cut(s) 348, 442, 477, 585
HphI GGTGA 4 cut(s) 428, 638, 826, 964
Hpy166II GTNNAC 2 cut(s) 973, 1052
Hpy188I TCNGA 8 cut(s) 86, 122, 184, 285, 296, 333, 759, 1046
Hpy8I GTNNAC 2 cut(s) 973, 1052
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 5 cut(s) 91, 338, 977, 1150, 1164
HpyCH4III ACNGT 4 cut(s) 264, 381, 487, 1060
HpyCH4IV ACGT 2 cut(s) 524, 925
HpyCH4V TGCA 4 cut(s) 367, 886, 973, 1041
HpyF10VI GCNNNNNNNGC 5 cut(s) 409, 553, 637, 733, 944
HpySE526I ACGT 2 cut(s) 524, 925
Hsp92I GRCGYC 2 cut(s) 249, 524
Hsp92II CATG 7 cut(s) 469, 505, 533, 659, 811, 870, 890
HspAI GCGC 3 cut(s) 36, 1084, 1156
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 5 cut(s) 179, 291, 715, 1173, 1204
LmnI GCTCC 3 cut(s) 430, 797, 910
Lsp1109I GCAGC 5 cut(s) 201, 301, 371, 424, 460
LweI GCATC 2 cut(s) 925, 1050
MaeI CTAG 4 cut(s) 219, 434, 567, 1010
MaeII ACGT 2 cut(s) 524, 925
MaeIII GTNAC 8 cut(s) 74, 353, 373, 862, 875, 1030, 1060, 1179
MalI GATC 5 cut(s) 181, 293, 717, 1175, 1206
MboI GATC 5 cut(s) 179, 291, 715, 1173, 1204
MboII GAAGA 4 cut(s) 8, 11, 162, 211
MflI RGATCY 2 cut(s) 715, 1204
MhlI GDGCHC 4 cut(s) 405, 483, 655, 975
MluCI AATT 2 cut(s) 87, 744
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 552
Mph1103I ATGCAT 2 cut(s) 888, 1043
MseI TTAA 2 cut(s) 732, 900
MslI CAYNNNNRTG 3 cut(s) 791, 812, 996
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 4 cut(s) 348, 442, 477, 585
MspR9I CCNGG 5 cut(s) 442, 443, 478, 637, 996
Mva1269I GAATGC 2 cut(s) 1043, 1186
MvaI CCWGG 2 cut(s) 637, 996
MvnI CGCG 1 cut(s) 1084
MwoI GCNNNNNNNGC 5 cut(s) 409, 553, 637, 733, 944
NciI CCSGG 3 cut(s) 442, 443, 478
NdeI CATATG 1 cut(s) 612
NdeII GATC 5 cut(s) 179, 291, 715, 1173, 1204
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 7 cut(s) 469, 505, 533, 659, 811, 870, 890
NlaIV GGNNCC 2 cut(s) 717, 906
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 7 cut(s) 74, 373, 862, 875, 1030, 1060, 1179
NsiI ATGCAT 2 cut(s) 888, 1043
PagI TCATGA 3 cut(s) 465, 501, 529
PctI GAATGC 2 cut(s) 1043, 1186
PfeI GAWTC 5 cut(s) 185, 462, 575, 693, 751
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 6 cut(s) 216, 316, 361, 439, 475, 1198
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 635, 994
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 2 cut(s) 635, 994
PspN4I GGNNCC 2 cut(s) 717, 906
PspPI GGNCC 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 2 cut(s) 715, 1204
RsaI GTAC 1 cut(s) 634
RsaNI GTAC 1 cut(s) 633
RseI CAYNNNNRTG 3 cut(s) 791, 812, 996
SaqAI TTAA 2 cut(s) 732, 900
SatI GCNGC 6 cut(s) 215, 315, 360, 438, 474, 1197
Sau3AI GATC 5 cut(s) 179, 291, 715, 1173, 1204
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 5 cut(s) 442, 443, 478, 637, 996
SduI GDGCHC 4 cut(s) 405, 483, 655, 975
SfaNI GCATC 2 cut(s) 925, 1050
SfcI CTRYAG 1 cut(s) 1208
SmaI CCCGGG 1 cut(s) 443
SmiMI CAYNNNNRTG 3 cut(s) 791, 812, 996
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
Sse9I AATT 2 cut(s) 87, 744
SsiI CCGC 4 cut(s) 158, 312, 844, 1196
SspMI CTAG 4 cut(s) 219, 434, 567, 1010
StyD4I CCNGG 5 cut(s) 440, 441, 476, 635, 994
StyI CCWWGG 4 cut(s) 457, 688, 1106, 1200
TaaI ACNGT 4 cut(s) 264, 381, 487, 1060
TaiI ACGT 2 cut(s) 527, 928
TaqI TCGA 3 cut(s) 192, 266, 933
TasI AATT 2 cut(s) 87, 744
TauI GCSGC 1 cut(s) 1199
TfiI GAWTC 5 cut(s) 185, 462, 575, 693, 751
Tru1I TTAA 2 cut(s) 732, 900
Tru9I TTAA 2 cut(s) 732, 900
TscAI CASTG 4 cut(s) 386, 517, 1063, 1103
TseFI GTSAC 7 cut(s) 74, 373, 862, 875, 1030, 1060, 1179
TseI GCWGC 5 cut(s) 214, 314, 359, 437, 473
Tsp45I GTSAC 7 cut(s) 74, 373, 862, 875, 1030, 1060, 1179
TspDTI ATGAA 3 cut(s) 454, 995, 1132
TspMI CCCGGG 1 cut(s) 441
TspRI CASTG 4 cut(s) 386, 517, 1063, 1103
Van91I CCANNNNNTGG 1 cut(s) 887
VneI GTGCAC 1 cut(s) 971
XapI RAATTY 2 cut(s) 87, 744
XbaI TCTAGA 1 cut(s) 1009
XmaI CCCGGG 1 cut(s) 441
XspI CTAG 4 cut(s) 219, 434, 567, 1010
ZraI GACGTC 1 cut(s) 525
Zsp2I ATGCAT 2 cut(s) 888, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.