pycom15g37540

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002499
Physical Location & Seq
Reverse (-)
37466200 .. 37470816
4617 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1158 bp
ATGTATTTTCCCAGAAGAGGTTCTCAGTTTACACCTCCGCATTATTCAGTTGATTTCTATTGGAAAGATTATTGCCCGATGGTCTTCAGGAATTTAAGGGAGATGTTTAAATTAGATGCTGCCGAGTACATGATGTCCATTTGTGGCGATGATGGTTTAAGAGAGCTTTCTTCTCCGGGGAAAAGTGGCAGTATCTTCTATCTTTCTCATGATGACAGATTTGTCATCAAGACTTTAAGAAAATCAGAACTGAAGGTTCTGCTCAAGATGCTGCCTAGCTATTATTGCCATGTAGGAGAACATGAAAACACTCTCATCACAAAATTTTTTGGGCTCCATCGGATAACATTAAAAGGTGGAAAAAAGGTACGGTTTATGGTCATGGGGAATATGTTTTGCACTGAATTAAGAATTCATCGCCGTTATGATTTGAAGGGTTCATCTATTGGAAGATGCACAGACAAAAATAAAATTAAAGAGAATACCACATTGAAGGATCTCGATCTGTCATTTGAATTTCATATGGACAAACTGTTGCGAGAAGCCCTTTTCAAGCAAATATCACTAGACTGCATGTTTCTACAATCTCAGCAGATTATCGATTATAGCCTTCTGTTGGGTCTACATTTTAGAGCTCCCGAGAATCTGAAGGGATTTTCACAACCTCCTGCTACAATGCACAATGATGAGAGTTTACCTACTAATGATGGTGTGACTTCTCAAGGGGAGCTTTTGATTCCTCCTAAAGGTCTTCTGCTGGTAACCCATGAGCCTGGCTCTGTCAGTACCGCACCAGGTCCTCACATCAGAGGAAGTACATTGAAAGCTTATTCTCTCGGTGACAAAGAAGTTGATCTTCTAGTCCCCGGTACAGGAAGGTTAAGAGTGCAATTAGGTGTAAACATGCCAGCCCAGGCCTATCATAAGCTTGCGCAGGACGAGGTCGATTCAACAGAAGTAGAACTTTTTGAATTTTACGACGTGGTTCTTTATATGGGGATAATTGATATACTGCAGGAATACAACGCGAAGAAGAAACTTGAGCATGCTTACAAATCATTGCGGTTTGATCCTCGATGCATTTCTGCTGTGGAACCAAAGCTGTATGCTAAGCGTTTCATCAATTTCTTGGAGAAGGTTTTTCCGGACCTGCCATGA

Protein Analysis

386

Amino Acids

44.23

Weight (kDa)

8.49

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 47 - 379 3.6e-98 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
Acc16I TGCGCA 1 cut(s) 933
AccI GTMKAC 1 cut(s) 622
AccII CGCG 1 cut(s) 1028
AccIII TCCGGA 1 cut(s) 1144
AciI CCGC 3 cut(s) 38, 789, 1063
AclWI GGATC 2 cut(s) 504, 1064
AcsI RAATTY 5 cut(s) 91, 323, 411, 515, 971
AcuI CTGAAG 3 cut(s) 70, 272, 668
AfaI GTAC 5 cut(s) 128, 369, 787, 817, 871
AfiI CCNNNNNNNGG 4 cut(s) 17, 616, 746, 872
AgsI TTSAA 7 cut(s) 433, 493, 515, 553, 823, 951, 971
AjiI CACGTC 1 cut(s) 982
AjnI CCWGG 3 cut(s) 772, 793, 912
AluBI AGCT 7 cut(s) 166, 279, 635, 730, 827, 928, 1102
AluI AGCT 7 cut(s) 166, 279, 635, 730, 827, 928, 1102
Alw21I GWGCWC 1 cut(s) 637
AlwI GGATC 2 cut(s) 504, 1064
Ama87I CYCGRG 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 1144
AoxI GGCC 1 cut(s) 915
ApeKI GCWGC 2 cut(s) 119, 271
ApoI RAATTY 5 cut(s) 91, 323, 411, 515, 971
Asp700I GAANNNNTTC 1 cut(s) 19
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 2 cut(s) 797, 1147
AsuC2I CCSGG 2 cut(s) 177, 867
AsuHPI GGTGA 1 cut(s) 851
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 2 cut(s) 797, 1147
BanII GRGCYC 2 cut(s) 336, 637
BbsI GAAGAC 2 cut(s) 76, 743
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 2 cut(s) 106, 258
BccI CCATC 4 cut(s) 73, 146, 345, 701
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 3 cut(s) 774, 795, 914
BcnI CCSGG 2 cut(s) 177, 867
BfaI CTAG 3 cut(s) 276, 566, 860
BfmI CTRYAG 1 cut(s) 1013
BisI GCNGC 2 cut(s) 120, 272
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 2 cut(s) 121, 273
Bme1390I CCNGG 5 cut(s) 177, 774, 795, 867, 914
Bme18I GGWCC 2 cut(s) 797, 1147
BmeT110I CYCGRG 1 cut(s) 638
BmgBI CACGTC 1 cut(s) 982
BmgT120I GGNCC 2 cut(s) 797, 1147
BmiI GGNNCC 2 cut(s) 335, 1095
BmrFI CCNGG 5 cut(s) 177, 774, 795, 867, 914
BmsI GCATC 4 cut(s) 106, 258, 443, 1067
BpiI GAAGAC 2 cut(s) 76, 743
BplI GAGNNNNNCTC 2 cut(s) 761, 793
Bpu1102I GCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 3 cut(s) 248, 705, 1061
BpuMI CCSGG 2 cut(s) 177, 867
Bsa29I ATCGAT 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 176, 865, 912
BsaWI WCCGGW 1 cut(s) 1144
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 616, 746, 872
Bse3DI GCAATG 1 cut(s) 1058
Bse8I GATNNNNATC 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 1144
BseBI CCWGG 3 cut(s) 774, 795, 914
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 176, 865, 912
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 4 cut(s) 17, 616, 746, 872
BseMI GCAATG 1 cut(s) 1058
BseMII CTCAG 2 cut(s) 38, 602
BseXI GCAGC 2 cut(s) 106, 258
Bsh1236I CGCG 1 cut(s) 1028
BshFI GGCC 1 cut(s) 917
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 3 cut(s) 176, 867, 1145
BslFI GGGAC 1 cut(s) 848
BslI CCNNNNNNNGG 4 cut(s) 17, 616, 746, 872
BsmFI GGGAC 1 cut(s) 848
BsnI GGCC 1 cut(s) 917
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 2 cut(s) 336, 637
Bsp13I TCCGGA 1 cut(s) 1144
Bsp143I GATC 4 cut(s) 496, 502, 853, 1069
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 3 cut(s) 38, 789, 1063
BspANI GGCC 1 cut(s) 917
BspCNI CTCAG 2 cut(s) 37, 601
BspDI ATCGAT 1 cut(s) 600
BspEI TCCGGA 1 cut(s) 1144
BspFNI CGCG 1 cut(s) 1028
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 335, 1095
BspMAI CTGCAG 1 cut(s) 1017
BspPI GGATC 2 cut(s) 504, 1064
BsrDI GCAATG 1 cut(s) 1058
BssECI CCNNGG 3 cut(s) 176, 865, 912
BssMI GATC 4 cut(s) 496, 502, 853, 1069
Bst2UI CCWGG 3 cut(s) 774, 795, 914
Bst4CI ACNGT 2 cut(s) 372, 534
Bst6I CTCTTC 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 909, 930, 1047
BstDEI CTNAG 3 cut(s) 24, 588, 1110
BstEII GGTNACC 1 cut(s) 760
BstENI CCTNNNNNAGG 1 cut(s) 744
BstFNI CGCG 1 cut(s) 1028
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 4 cut(s) 499, 505, 856, 1072
BstMBI GATC 4 cut(s) 496, 502, 853, 1069
BstMWI GCNNNNNNNGC 2 cut(s) 268, 285
BstNI CCWGG 3 cut(s) 774, 795, 914
BstNSI RCATGY 3 cut(s) 577, 907, 1049
BstPI GGTNACC 1 cut(s) 760
BstSCI CCNGG 5 cut(s) 175, 772, 793, 865, 912
BstSFI CTRYAG 1 cut(s) 1013
BstUI CGCG 1 cut(s) 1028
BstV1I GCAGC 2 cut(s) 106, 258
BstV2I GAAGAC 2 cut(s) 76, 743
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 773
BstYI RGATCY 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 600
BsuRI GGCC 1 cut(s) 917
BsuTUI ATCGAT 1 cut(s) 600
BtgZI GCGATG 2 cut(s) 162, 401
BtrI CACGTC 1 cut(s) 982
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 3 cut(s) 909, 930, 1047
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 934
Cfr13I GGNCC 2 cut(s) 797, 1147
ClaI ATCGAT 1 cut(s) 600
CsiI ACCWGGT 1 cut(s) 793
Csp6I GTAC 5 cut(s) 127, 368, 786, 816, 870
CviQI GTAC 5 cut(s) 127, 368, 786, 816, 870
DdeI CTNAG 3 cut(s) 24, 588, 1110
DpnI GATC 4 cut(s) 498, 504, 855, 1071
DpnII GATC 4 cut(s) 496, 502, 853, 1069
DraI TTTAAA 1 cut(s) 109
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 635
Eco147I AGGCCT 1 cut(s) 917
Eco24I GRGCYC 2 cut(s) 336, 637
Eco47I GGWCC 2 cut(s) 797, 1147
Eco53kI GAGCTC 1 cut(s) 635
Eco57I CTGAAG 3 cut(s) 70, 272, 668
Eco88I CYCGRG 1 cut(s) 638
Eco91I GGTNACC 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 797
EcoO65I GGTNACC 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 3 cut(s) 772, 793, 912
EcoT22I ATGCAT 1 cut(s) 1082
EcoT38I GRGCYC 2 cut(s) 336, 637
FalI AAGNNNNNCTT 6 cut(s) 714, 746, 840, 872, 948, 980
FaqI GGGAC 1 cut(s) 848
FauNDI CATATG 1 cut(s) 522
FblI GTMKAC 1 cut(s) 622
Fnu4HI GCNGC 2 cut(s) 120, 272
FriOI GRGCYC 2 cut(s) 336, 637
Fsp4HI GCNGC 2 cut(s) 120, 272
FspBI CTAG 3 cut(s) 276, 566, 860
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 1 cut(s) 933
GluI GCNGC 2 cut(s) 120, 272
HaeIII GGCC 1 cut(s) 917
HapII CCGG 3 cut(s) 176, 867, 1145
HhaI GCGC 1 cut(s) 934
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HindIII AAGCTT 2 cut(s) 825, 926
HinfI GANTC 3 cut(s) 643, 736, 947
HpaII CCGG 3 cut(s) 176, 867, 1145
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 4 cut(s) 30, 623, 695, 901
Hpy188I TCNGA 4 cut(s) 247, 342, 648, 809
Hpy188III TCNNGA 7 cut(s) 88, 209, 229, 265, 500, 638, 1145
Hpy8I GTNNAC 4 cut(s) 30, 623, 695, 901
Hpy99I CGWCG 1 cut(s) 983
HpyAV CCTTC 7 cut(s) 247, 427, 487, 620, 643, 870, 1129
HpyCH4III ACNGT 2 cut(s) 372, 534
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 7 cut(s) 399, 456, 573, 679, 889, 1015, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 285
HpyF3I CTNAG 3 cut(s) 24, 588, 1110
HpySE526I ACGT 1 cut(s) 981
HspAI GCGC 1 cut(s) 932
Kpn2I TCCGGA 1 cut(s) 1144
Kzo9I GATC 4 cut(s) 496, 502, 853, 1069
LmnI GCTCC 3 cut(s) 339, 640, 727
Lsp1109I GCAGC 2 cut(s) 106, 258
LweI GCATC 4 cut(s) 106, 258, 443, 1067
MabI ACCWGGT 1 cut(s) 793
MaeI CTAG 3 cut(s) 276, 566, 860
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 3 cut(s) 712, 760, 839
MalI GATC 4 cut(s) 498, 504, 855, 1071
MboI GATC 4 cut(s) 496, 502, 853, 1069
MboII GAAGA 9 cut(s) 27, 76, 162, 187, 462, 743, 848, 1042, 1045
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 336, 637
MnlI CCTC 8 cut(s) 11, 45, 675, 750, 803, 810, 934, 1083
Mph1103I ATGCAT 1 cut(s) 1082
MroI TCCGGA 1 cut(s) 1144
MroXI GAANNNNTTC 1 cut(s) 19
MseI TTAA 8 cut(s) 95, 108, 158, 236, 350, 407, 474, 881
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 3 cut(s) 176, 867, 1145
MspR9I CCNGG 5 cut(s) 177, 774, 795, 867, 914
MvaI CCWGG 3 cut(s) 774, 795, 914
MvnI CGCG 1 cut(s) 1028
MwoI GCNNNNNNNGC 2 cut(s) 268, 285
NciI CCSGG 2 cut(s) 177, 867
NdeI CATATG 1 cut(s) 522
NdeII GATC 4 cut(s) 496, 502, 853, 1069
NlaIV GGNNCC 2 cut(s) 335, 1095
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 712, 839
NsbI TGCGCA 1 cut(s) 933
NsiI ATGCAT 1 cut(s) 1082
NspI RCATGY 3 cut(s) 577, 907, 1049
PaeI GCATGC 1 cut(s) 1049
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 1 cut(s) 917
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 643, 736, 947
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 2 cut(s) 121, 273
PpuMI RGGWCCY 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 797
Psp6I CCWGG 3 cut(s) 772, 793, 912
PspEI GGTNACC 1 cut(s) 760
PspGI CCWGG 3 cut(s) 772, 793, 912
PspN4I GGNNCC 2 cut(s) 335, 1095
PspPI GGNCC 2 cut(s) 797, 1147
PspPPI RGGWCCY 1 cut(s) 797
PstI CTGCAG 1 cut(s) 1017
PsuI RGATCY 1 cut(s) 496
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 5 cut(s) 128, 369, 787, 817, 871
RsaNI GTAC 5 cut(s) 127, 368, 786, 816, 870
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 637
SaqAI TTAA 8 cut(s) 95, 108, 158, 236, 350, 407, 474, 881
SatI GCNGC 2 cut(s) 120, 272
Sau3AI GATC 4 cut(s) 496, 502, 853, 1069
Sau96I GGNCC 2 cut(s) 797, 1147
ScrFI CCNGG 5 cut(s) 177, 774, 795, 867, 914
SduI GDGCHC 2 cut(s) 336, 637
SexAI ACCWGGT 1 cut(s) 793
SfaNI GCATC 4 cut(s) 106, 258, 443, 1067
SfcI CTRYAG 1 cut(s) 1013
SinI GGWCC 2 cut(s) 797, 1147
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 3 cut(s) 263, 720, 1040
SmoI CTYRAG 3 cut(s) 263, 720, 1040
SphI GCATGC 1 cut(s) 1049
SseBI AGGCCT 1 cut(s) 917
SsiI CCGC 3 cut(s) 38, 789, 1063
SspMI CTAG 3 cut(s) 276, 566, 860
SstI GAGCTC 1 cut(s) 637
StuI AGGCCT 1 cut(s) 917
StyD4I CCNGG 5 cut(s) 175, 772, 793, 865, 912
TaaI ACNGT 2 cut(s) 372, 534
TaiI ACGT 1 cut(s) 984
TaqI TCGA 4 cut(s) 501, 600, 945, 1075
TatI WGTACW 2 cut(s) 126, 815
TfiI GAWTC 3 cut(s) 643, 736, 947
Tru1I TTAA 8 cut(s) 95, 108, 158, 236, 350, 407, 474, 881
Tru9I TTAA 8 cut(s) 95, 108, 158, 236, 350, 407, 474, 881
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 712, 839
TseI GCWGC 2 cut(s) 119, 271
Tsp45I GTSAC 2 cut(s) 712, 839
TspDTI ATGAA 5 cut(s) 318, 404, 429, 509, 1108
TspRI CASTG 1 cut(s) 406
Tth111I GACNNNGTC 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 797, 1147
XagI CCTNNNNNAGG 1 cut(s) 744
XapI RAATTY 5 cut(s) 91, 323, 411, 515, 971
XceI RCATGY 3 cut(s) 577, 907, 1049
XmiI GTMKAC 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 3 cut(s) 276, 566, 860
Zsp2I ATGCAT 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.