pycom15g38670
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017871
Physical Location & Seq
Reverse (-)
38435205 .. 38436222
1018 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGCAATGCAAACCAGTCATTCGACAGCACCAAAATGAGGCTATGGTGCCAACAACCCTAATTCACGAAAGACTTTCAAATGCAGCTACTGTGACGGAGGACATCCAGTCGAATGTTGTTTTTTCCTTATTGGCTTCCTTAAAGGACACAAATGGCACGGGAAGAATGTGCAGCCCAGAAATAGGCGTGCACCTCCAGCCTCCAACAATGTTGAAACACTACCGTTGCCAGCTGCTCAGATTGACCCAACCAAAGCAACAACCTCCAACAATCAACCAACGTTCACAACCGAGGAATACCATCAACTCATGGCCCTTCTTCGCAATAAGACTGGTACCAATCTGCCACTTGTACATGCAACAGGACATGGCTACAAAGAGGATGATTGGCCTGGGCAAGCAGTTTAATGGACTCTACTACCTCACTCCAACACAAAACCCTCATCTCACCCACCATGTCAACCGTACCTCGGCACTGTGGCACCAACGTCTTGGGCACCCATCCTCAACTCCTCTTCACTCTTTATCCCAGACCATTCCAGAAATTATTTTTGATTTTACACATGTTTGTGATGTTTGTCCTTTAGCAAAGCAAACTCGTTCATCTTTTGTTTCCAGTTCAATAAAATCAGTTCCATACACCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

216

Amino Acids

24.44

Weight (kDa)

9.65

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 137 - 198 1.5e-11 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017871)

Species Orthologous Gene IDs
malus_domestica MD09G1281800.v1.1
pyrus_communis pycom12g08370 pycom15g03640 pycom15g18740 pycom15g38670
rosa_chinensis RchiOBHm_Chr1g0356321
rosa_multiflora Rmu_sc0000605.1_g000016
rosa_roxburghii Rroxscaffold_2G00133080 Rroxscaffold_4G00285200 Rroxscaffold_7G00191550
rosa_wichuraiana Rw2G001150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 334
AccB1I GGYRCC 4 cut(s) 46, 334, 480, 495
AclI AACGTT 1 cut(s) 280
AfaI GTAC 3 cut(s) 336, 353, 466
AfiI CCNNNNNNNGG 3 cut(s) 37, 182, 469
AflIII ACRYGT 1 cut(s) 562
AgsI TTSAA 3 cut(s) 78, 214, 621
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 2 cut(s) 86, 232
AluI AGCT 2 cut(s) 86, 232
Alw21I GWGCWC 1 cut(s) 192
Alw44I GTGCAC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 2 cut(s) 311, 388
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 3 cut(s) 83, 171, 232
Asp718I GGTACC 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 439
BaeGI GKGCMC 2 cut(s) 192, 498
BanI GGYRCC 4 cut(s) 46, 334, 480, 495
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 95, 183, 219
BccI CCATC 2 cut(s) 308, 508
BciT130I CCWGG 1 cut(s) 392
BfaI CTAG 1 cut(s) 646
BisI GCNGC 3 cut(s) 84, 172, 233
BlsI GCNGC 3 cut(s) 85, 173, 234
Bme1390I CCNGG 1 cut(s) 392
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 4 cut(s) 48, 336, 482, 497
BmrFI CCNGG 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 179
BsaJI CCNNGG 3 cut(s) 290, 391, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 182, 469
Bse1I ACTGG 4 cut(s) 14, 106, 336, 615
Bse3DI GCAATG 1 cut(s) 11
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 3 cut(s) 290, 391, 468
BseGI GGATG 3 cut(s) 102, 387, 500
BseLI CCNNNNNNNGG 3 cut(s) 37, 182, 469
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 4 cut(s) 14, 106, 336, 615
BseRI GAGGAG 1 cut(s) 501
BseSI GKGCMC 2 cut(s) 192, 498
BseXI GCAGC 3 cut(s) 95, 183, 219
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 2 cut(s) 313, 390
BshNI GGYRCC 4 cut(s) 46, 334, 480, 495
BsiHKAI GWGCWC 1 cut(s) 192
BslI CCNNNNNNNGG 3 cut(s) 37, 182, 469
BsnI GGCC 2 cut(s) 313, 390
Bsp1286I GDGCHC 2 cut(s) 192, 498
Bsp1407I TGTACA 1 cut(s) 351
BspANI GGCC 2 cut(s) 313, 390
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 48, 336, 482, 497
BspT107I GGYRCC 4 cut(s) 46, 334, 480, 495
BsrDI GCAATG 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 4 cut(s) 14, 106, 336, 615
BssECI CCNNGG 3 cut(s) 290, 391, 468
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 4 cut(s) 91, 224, 464, 477
Bst6I CTCTTC 1 cut(s) 519
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 188, 230, 398
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 3 cut(s) 102, 387, 500
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 2 cut(s) 358, 566
BstSCI CCNGG 1 cut(s) 390
BstSLI GKGCMC 2 cut(s) 192, 498
BstV1I GCAGC 3 cut(s) 95, 183, 219
BstXI CCANNNNNNTGG 1 cut(s) 491
BsuRI GGCC 2 cut(s) 313, 390
BtsCI GGATG 3 cut(s) 102, 387, 500
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 3 cut(s) 188, 230, 398
CaiI CAGNNNCTG 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 3 cut(s) 335, 352, 465
CviAII CATG 5 cut(s) 309, 355, 367, 455, 563
CviJI RGCY 9 cut(s) 41, 86, 134, 174, 199, 232, 313, 371, 390
CviKI_1 RGCY 9 cut(s) 41, 86, 134, 174, 199, 232, 313, 371, 390
CviQI GTAC 3 cut(s) 335, 352, 465
DdeI CTNAG 1 cut(s) 236
DriI GACNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 519
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 312, 358, 370, 458, 566
FaiI YATR 7 cut(s) 44, 310, 356, 368, 456, 564, 637
FatI CATG 5 cut(s) 308, 354, 366, 454, 562
Fnu4HI GCNGC 3 cut(s) 84, 172, 233
FokI GGATG 3 cut(s) 89, 394, 487
Fsp4HI GCNGC 3 cut(s) 84, 172, 233
FspBI CTAG 1 cut(s) 646
GluI GCNGC 3 cut(s) 84, 172, 233
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 2 cut(s) 313, 390
Hin1II CATG 5 cut(s) 312, 358, 370, 458, 566
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 1 cut(s) 411
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 3 cut(s) 190, 284, 460
Hpy188I TCNGA 1 cut(s) 239
Hpy188III TCNNGA 2 cut(s) 65, 539
Hpy8I GTNNAC 3 cut(s) 190, 284, 460
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 4 cut(s) 91, 224, 464, 477
HpyCH4IV ACGT 2 cut(s) 280, 487
HpyCH4V TGCA 6 cut(s) 4, 9, 83, 171, 190, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 2 cut(s) 280, 487
Hsp92II CATG 5 cut(s) 312, 358, 370, 458, 566
KpnI GGTACC 1 cut(s) 338
Lsp1109I GCAGC 3 cut(s) 95, 183, 219
MaeI CTAG 1 cut(s) 646
MaeII ACGT 2 cut(s) 280, 487
MaeIII GTNAC 1 cut(s) 91
MboII GAAGA 3 cut(s) 174, 310, 506
MhlI GDGCHC 2 cut(s) 192, 498
MluCI AATT 2 cut(s) 60, 543
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 3 cut(s) 227, 290, 452
MseI TTAA 2 cut(s) 140, 405
MslI CAYNNNNRTG 2 cut(s) 33, 567
MspA1I CMGCKG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 392
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 196
NlaIII CATG 5 cut(s) 312, 358, 370, 458, 566
NlaIV GGNNCC 4 cut(s) 48, 336, 482, 497
NmeAIII GCCGAG 1 cut(s) 449
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 2 cut(s) 358, 566
PciI ACATGT 1 cut(s) 562
PkrI GCNGC 3 cut(s) 85, 173, 234
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PscI ACATGT 1 cut(s) 562
Psp1406I AACGTT 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 4 cut(s) 48, 336, 482, 497
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 232
RsaI GTAC 3 cut(s) 336, 353, 466
RsaNI GTAC 3 cut(s) 335, 352, 465
RseI CAYNNNNRTG 2 cut(s) 33, 567
SaqAI TTAA 2 cut(s) 140, 405
SatI GCNGC 3 cut(s) 84, 172, 233
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 2 cut(s) 192, 498
SetI ASST 8 cut(s) 88, 195, 234, 265, 283, 423, 470, 490
SmiMI CAYNNNNRTG 2 cut(s) 33, 567
Sse9I AATT 2 cut(s) 60, 543
SspMI CTAG 1 cut(s) 646
StyD4I CCNGG 1 cut(s) 390
TaaI ACNGT 4 cut(s) 91, 224, 464, 477
TaiI ACGT 2 cut(s) 283, 490
TaqI TCGA 2 cut(s) 22, 110
TasI AATT 2 cut(s) 60, 543
TatI WGTACW 1 cut(s) 351
Tru1I TTAA 2 cut(s) 140, 405
Tru9I TTAA 2 cut(s) 140, 405
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 83, 171, 232
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 591
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 480
VneI GTGCAC 1 cut(s) 188
XceI RCATGY 2 cut(s) 358, 566
XspI CTAG 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.