pycom15g38970

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016048
Physical Location & Seq
Forward (+)
38660261 .. 38662665
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGGATTCAGATAAACCCAGCAGCTTATTTCTGGGTAAGGCGATGAAGCCCACGTGGGCATCTCTGCTTTTCGTACTCACCATCATGGTTGTCCTTTCCTACCAAAACTTCTCGACGAGGTCGAGGCTTCCGACATGGGTCGGGTCGATTTGGCCAACTGGAGGATCCGAAACCCGATCGGATGCCCCGGCCAGCAGCTGTGCCGGGTTCTTCGGGGATGTGCCGAAGCGGAAGGTGGTGATGTCCATCGAAGCTTTCGGTGGCGTTGGCGACGGAACCACCTCCAACACGGAAGCGTTTAGGAGGGCGATACTCCACATGCAGGCTTTCCGTAATACCGGTGGGTCCCAGTTGAATGTCCCCAAGGGAAGATGGCTGACGGGGAGTTTTAACCTCACCAGTAATTTCACGCTTTTTCTTGAACACGGCGCTGTAATTCTCGGCTCCCAGGATCCGAAGCAATGGCCTGTAATTGAGCCGTTGCCTTCTTATGGAAGAGGGAGAGAGAGGTTAGGAGGAAGGCATATAAGCCTTGTTCATGGGGATGGTCTCACAAATGTTGTCATTACAGGTCATAACGGGACGGTTGACGGTCAGGGAAAAATGTGGTGGGAACTATGGTGGAACAGAACATTGGAGCACACCAGAGGTCACCTCCTTGAAATAAAAAACTCTCGCAACATTCTCATCTCTAACCTCACCTTCCGCAATTCTCCCTTTTGGACCATCCATCCTGTTTACTGCAGTAACGTTGTTATCAAGGACATGACAATCTTGGCCCCCCTTAACGCTCCTAATACTGATGGTATAGACCCAGATTCCAGTACTAATGTCTGCATTCAAGACTGTTACATAGAGAGTGGCGACGATCTTGTAGCAGTGAAAAGTGGGTGGGATCACTATGGAATCAAAATGGCTCGTCCAAGCTCAAACATAATAATTAGAAGAGTTAATGGCACCACTCCAACTTGCTCTGGAGTGGGAATAGGTAGTGAGATGTCTGGTGGGATTTCAAATATCACCATTGAGGATTTGCATGTTTGGGATTCCGCAGCTGGGGTGCGGATTAAATCTGATAAGGGTAGAGGGGGTTACATAGCGAATGTTAGTATAAGTAACTTAATGATGGAAAGGGTGAAGATACCCATAAGGTTTAGTAGAGGGGCGAATGATCACCCGGATAAAGGATGGGATCCCAAAGCTGTCCCCCATGTAAAGGGGATTTTTATTAGTAATGTGCTGAGTTTGAATTCAACAAAAGCCCCAGAATTGAAGGGCATTGAGGGGGCATCATTTGAAAGGATATGCTTTAAGAATGTCACAGTACTTGGTCTGGCTCCATCTGCAGCGTGGCATTGTGAATTTGTTTTGGGTTTTGCAGATGGGGTATTTCCAGTGCCATGTCCTCAGATGGAGGATATGGACTCGTTTGGCTCTTGTATGCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

484

Amino Acids

52.75

Weight (kDa)

8.32

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 178 - 454 6.2e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33440
fragaria_vesca FvH4_1g24350
malus_domestica MD15G1441700.v1.1
prunus_persica Prupe.4G271300_v2.0.a1
pyrus_communis pycom15g38970
rosa_chinensis RchiOBHm_Chr2g0120901
rosa_laevigata RLG00000018600
rosa_multiflora Rmu_sc0000621.1_g000016
rosa_roxburghii Rroxscaffold_2G00122750
rosa_rugosa Rorug02G0232600
rosa_samantha Rh2AG289000 Rh2BG300000 Rh2CG278000 Rh2DG314000
rosa_wichuraiana Rw2G023150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 956
AciI CCGC 4 cut(s) 229, 706, 1050, 1063
AclI AACGTT 1 cut(s) 750
AclWI GGATC 7 cut(s) 159, 172, 446, 459, 903, 1187, 1200
AcoI YGGCCR 2 cut(s) 152, 189
AcsI RAATTY 2 cut(s) 1249, 1361
AcvI CACGTG 1 cut(s) 54
AfaI GTAC 3 cut(s) 75, 826, 1326
AfiI CCNNNNNNNGG 6 cut(s) 161, 322, 491, 1056, 1184, 1216
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 9 cut(s) 355, 422, 662, 842, 1014, 1249, 1254, 1273, 1298
AjnI CCWGG 1 cut(s) 447
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 6 cut(s) 24, 198, 254, 927, 1055, 1202
AluI AGCT 6 cut(s) 24, 198, 254, 927, 1055, 1202
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 7 cut(s) 159, 172, 446, 459, 903, 1187, 1200
AlwNI CAGNNNCTG 1 cut(s) 198
AoxI GGCC 4 cut(s) 152, 189, 464, 777
ApeKI GCWGC 5 cut(s) 21, 195, 1052, 1346, 1444
ApoI RAATTY 2 cut(s) 1249, 1361
AsiGI ACCGGT 1 cut(s) 338
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 3 cut(s) 345, 723, 778
AsuC2I CCSGG 3 cut(s) 188, 205, 1178
AsuHPI GGTGA 8 cut(s) 70, 250, 388, 644, 691, 1012, 1147, 1166
AvaII GGWCC 2 cut(s) 345, 723
BalI TGGCCA 1 cut(s) 154
BamHI GGATCC 3 cut(s) 164, 451, 1192
BanI GGYRCC 1 cut(s) 956
BbrPI CACGTG 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 4 cut(s) 33, 207, 1064, 1358
BceAI ACGGC 2 cut(s) 442, 463
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 1171
BcnI CCSGG 3 cut(s) 188, 205, 1178
BcoDI GTCTC 1 cut(s) 554
BfmI CTRYAG 2 cut(s) 742, 1344
BfoI RGCGCY 1 cut(s) 432
BisI GCNGC 5 cut(s) 22, 196, 1053, 1347, 1445
BlsI GCNGC 5 cut(s) 23, 197, 1054, 1348, 1446
BmcAI AGTACT 2 cut(s) 826, 1326
Bme1390I CCNGG 4 cut(s) 188, 205, 449, 1178
Bme18I GGWCC 2 cut(s) 345, 723
BmgT120I GGNCC 3 cut(s) 345, 723, 778
BmrFI CCNGG 4 cut(s) 188, 205, 449, 1178
BmrI ACTGGG 1 cut(s) 343
BmsI GCATC 3 cut(s) 68, 172, 1298
BmuI ACTGGG 1 cut(s) 343
BoxI GACNNNNGTC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 639, 671
BpmI CTGGAG 2 cut(s) 180, 996
BpuMI CCSGG 3 cut(s) 188, 205, 1178
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 186, 363, 447
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 322, 491, 1056, 1184, 1216
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 5 cut(s) 163, 349, 399, 822, 1394
Bse3DI GCAATG 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 3 cut(s) 186, 363, 447
BseGI GGATG 6 cut(s) 187, 223, 550, 726, 730, 1193
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 6 cut(s) 161, 322, 491, 1056, 1184, 1216
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 2 cut(s) 1232, 1421
BseNI ACTGG 5 cut(s) 163, 349, 399, 822, 1394
BseXI GCAGC 4 cut(s) 33, 207, 1064, 1358
BseYI CCCAGC 2 cut(s) 17, 1055
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 4 cut(s) 154, 191, 466, 779
BshNI GGYRCC 1 cut(s) 956
BshTI ACCGGT 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 4 cut(s) 188, 204, 339, 1178
BslFI GGGAC 4 cut(s) 331, 344, 595, 1190
BslI CCNNNNNNNGG 6 cut(s) 161, 322, 491, 1056, 1184, 1216
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 4 cut(s) 331, 344, 595, 1190
BsmI GAATGC 1 cut(s) 837
BsnI GGCC 4 cut(s) 154, 191, 466, 779
Bso31I GGTCTC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
BspACI CCGC 4 cut(s) 229, 706, 1050, 1063
BspANI GGCC 4 cut(s) 154, 191, 466, 779
BspCNI CTCAG 2 cut(s) 1233, 1420
BspMAI CTGCAG 2 cut(s) 746, 1348
BspPI GGATC 7 cut(s) 159, 172, 446, 459, 903, 1187, 1200
BspT107I GGYRCC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 5 cut(s) 163, 349, 399, 822, 1394
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 186, 363, 447
BssMI GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 4 cut(s) 586, 593, 848, 1324
Bst6I CTCTTC 2 cut(s) 490, 940
BstBAI YACGTR 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 193, 324
BstDEI CTNAG 2 cut(s) 1241, 1407
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 6 cut(s) 187, 223, 550, 726, 730, 1193
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 7 cut(s) 167, 179, 454, 871, 898, 1174, 1195
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 1 cut(s) 1343
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 322, 1040
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 4 cut(s) 186, 203, 447, 1176
BstSFI CTRYAG 2 cut(s) 742, 1344
BstV1I GCAGC 4 cut(s) 33, 207, 1064, 1358
BstX2I RGATCY 3 cut(s) 164, 451, 1192
BstYI RGATCY 3 cut(s) 164, 451, 1192
BsuRI GGCC 4 cut(s) 154, 191, 466, 779
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 6 cut(s) 187, 223, 550, 726, 730, 1193
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 885, 1401
Cac8I GCNNGC 2 cut(s) 193, 324
CaiI CAGNNNCTG 1 cut(s) 198
CfoI GCGC 1 cut(s) 431
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 345, 723, 778
Csp6I GTAC 3 cut(s) 74, 825, 1325
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 8 cut(s) 85, 135, 319, 539, 766, 1037, 1211, 1401
CviQI GTAC 3 cut(s) 74, 825, 1325
DdeI CTNAG 2 cut(s) 1241, 1407
DpnI GATC 7 cut(s) 166, 178, 453, 870, 897, 1173, 1194
DpnII GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
EaeI YGGCCR 2 cut(s) 152, 189
Eam1104I CTCTTC 2 cut(s) 490, 940
EarI CTCTTC 2 cut(s) 490, 940
Eco130I CCWWGG 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 2 cut(s) 345, 723
Eco72I CACGTG 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 345
EcoO65I GGTNACC 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 8 cut(s) 88, 138, 322, 542, 769, 1040, 1214, 1404
FaqI GGGAC 4 cut(s) 331, 344, 595, 1190
FatI CATG 8 cut(s) 84, 134, 318, 538, 765, 1036, 1210, 1400
FbaI TGATCA 1 cut(s) 1171
Fnu4HI GCNGC 5 cut(s) 22, 196, 1053, 1347, 1445
FokI GGATG 6 cut(s) 194, 230, 557, 713, 717, 1200
Fsp4HI GCNGC 5 cut(s) 22, 196, 1053, 1347, 1445
GlaI GCGC 1 cut(s) 430
GluI GCNGC 5 cut(s) 22, 196, 1053, 1347, 1445
GsaI CCCAGC 2 cut(s) 21, 1059
GsuI CTGGAG 2 cut(s) 180, 996
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 4 cut(s) 154, 191, 466, 779
HapII CCGG 4 cut(s) 188, 204, 339, 1178
HhaI GCGC 1 cut(s) 431
Hin1II CATG 8 cut(s) 88, 138, 322, 542, 769, 1040, 1214, 1404
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 5 cut(s) 5, 818, 906, 1046, 1424
HpaII CCGG 4 cut(s) 188, 204, 339, 1178
HphI GGTGA 8 cut(s) 70, 250, 388, 644, 691, 1012, 1147, 1166
Hpy166II GTNNAC 2 cut(s) 589, 739
Hpy188I TCNGA 7 cut(s) 10, 132, 169, 181, 456, 1075, 1410
Hpy188III TCNNGA 4 cut(s) 112, 419, 842, 975
Hpy8I GTNNAC 2 cut(s) 589, 739
Hpy99I CGWCG 3 cut(s) 118, 275, 869
HpyAV CCTTC 5 cut(s) 226, 495, 513, 712, 1267
HpyCH4III ACNGT 4 cut(s) 586, 593, 848, 1324
HpyCH4IV ACGT 2 cut(s) 53, 750
HpyCH4V TGCA 7 cut(s) 322, 744, 837, 1036, 1346, 1379, 1444
HpyF10VI GCNNNNNNNGC 1 cut(s) 1343
HpyF3I CTNAG 2 cut(s) 1241, 1407
HpySE526I ACGT 2 cut(s) 53, 750
Hsp92II CATG 8 cut(s) 88, 138, 322, 542, 769, 1040, 1214, 1404
HspAI GCGC 1 cut(s) 429
KflI GGGWCCC 1 cut(s) 345
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
LmnI GCTCC 4 cut(s) 449, 637, 796, 1342
Lsp1109I GCAGC 4 cut(s) 33, 207, 1064, 1358
LweI GCATC 3 cut(s) 68, 172, 1298
MaeII ACGT 2 cut(s) 53, 750
MaeIII GTNAC 6 cut(s) 650, 746, 848, 1091, 1115, 1318
MalI GATC 7 cut(s) 166, 178, 453, 870, 897, 1173, 1194
MboI GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
MboII GAAGA 5 cut(s) 202, 381, 507, 957, 1150
MflI RGATCY 3 cut(s) 164, 451, 1192
MhlI GDGCHC 1 cut(s) 642
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 8 cut(s) 403, 435, 471, 709, 939, 1249, 1268, 1361
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 1 cut(s) 1418
MmeI TCCRAC 3 cut(s) 155, 309, 989
Mox20I TGGCCA 1 cut(s) 154
MscI TGGCCA 1 cut(s) 154
MseI TTAA 6 cut(s) 390, 786, 951, 1068, 1121, 1311
MslI CAYNNNNRTG 2 cut(s) 83, 543
Msp20I TGGCCA 1 cut(s) 154
MspA1I CMGCKG 2 cut(s) 198, 1055
MspI CCGG 4 cut(s) 188, 204, 339, 1178
MspR9I CCNGG 4 cut(s) 188, 205, 449, 1178
Mva1269I GAATGC 1 cut(s) 837
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 1343
NciI CCSGG 3 cut(s) 188, 205, 1178
NdeII GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
NlaIII CATG 8 cut(s) 88, 138, 322, 542, 769, 1040, 1214, 1404
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 2 cut(s) 650, 1318
NspI RCATGY 2 cut(s) 322, 1040
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 128
PctI GAATGC 1 cut(s) 837
PfeI GAWTC 4 cut(s) 5, 818, 906, 1046
PflFI GACNNNGTC 1 cut(s) 118
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 5 cut(s) 23, 197, 1054, 1348, 1446
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 1 cut(s) 1418
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
PpsI GAGTC 1 cut(s) 1418
Ppu21I YACGTR 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 137
Psp1406I AACGTT 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 447
PspCI CACGTG 1 cut(s) 54
PspEI GGTNACC 1 cut(s) 650
PspFI CCCAGC 2 cut(s) 17, 1055
PspGI CCWGG 1 cut(s) 447
PspPI GGNCC 3 cut(s) 345, 723, 778
PspPPI RGGWCCY 1 cut(s) 345
PstI CTGCAG 2 cut(s) 746, 1348
PstNI CAGNNNCTG 1 cut(s) 198
PsuI RGATCY 3 cut(s) 164, 451, 1192
PsyI GACNNNGTC 1 cut(s) 118
PvuI CGATCG 1 cut(s) 179
PvuII CAGCTG 2 cut(s) 198, 1055
RsaI GTAC 3 cut(s) 75, 826, 1326
RsaNI GTAC 3 cut(s) 74, 825, 1325
RseI CAYNNNNRTG 2 cut(s) 83, 543
SaqAI TTAA 6 cut(s) 390, 786, 951, 1068, 1121, 1311
SatI GCNGC 5 cut(s) 22, 196, 1053, 1347, 1445
Sau3AI GATC 7 cut(s) 164, 176, 451, 868, 895, 1171, 1192
Sau96I GGNCC 3 cut(s) 345, 723, 778
ScaI AGTACT 2 cut(s) 826, 1326
SchI GAGTC 1 cut(s) 1418
ScrFI CCNGG 4 cut(s) 188, 205, 449, 1178
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 3 cut(s) 68, 172, 1298
SfcI CTRYAG 2 cut(s) 742, 1344
SinI GGWCC 2 cut(s) 345, 723
SmiMI CAYNNNNRTG 2 cut(s) 83, 543
Sse9I AATT 8 cut(s) 403, 435, 471, 709, 939, 1249, 1268, 1361
SsiI CCGC 4 cut(s) 229, 706, 1050, 1063
StyD4I CCNGG 4 cut(s) 186, 203, 447, 1176
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 4 cut(s) 586, 593, 848, 1324
TaiI ACGT 2 cut(s) 56, 753
TaqI TCGA 4 cut(s) 113, 122, 146, 249
TasI AATT 8 cut(s) 403, 435, 471, 709, 939, 1249, 1268, 1361
TatI WGTACW 2 cut(s) 824, 1324
TfiI GAWTC 4 cut(s) 5, 818, 906, 1046
Tru1I TTAA 6 cut(s) 390, 786, 951, 1068, 1121, 1311
Tru9I TTAA 6 cut(s) 390, 786, 951, 1068, 1121, 1311
TscAI CASTG 2 cut(s) 885, 1401
TseFI GTSAC 2 cut(s) 650, 1318
TseI GCWGC 5 cut(s) 21, 195, 1052, 1346, 1444
Tsp45I GTSAC 2 cut(s) 650, 1318
TspDTI ATGAA 2 cut(s) 59, 527
TspGWI ACGGA 3 cut(s) 288, 305, 320
TspRI CASTG 2 cut(s) 885, 1401
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 345, 723
XapI RAATTY 2 cut(s) 1249, 1361
XceI RCATGY 2 cut(s) 322, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 1347
ZrmI AGTACT 2 cut(s) 826, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.