pycom16g03310

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014698
Physical Location & Seq
Reverse (-)
2061127 .. 2061836
710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGACGTCTGGCCACAGAGACAGACATGTAAGGACTTGTGGACTTGTCTTAGCATATTACAGAATCAAGATTGGGAGATGGTTCGATTGTTACAACCTTGAGATTAGCTTGTTGAATCCGTTCTTTAGGAAGAAAACTGATCTTCCCAAAACATGGAAATTTTATCACAAGATGGTTCTCTCAGCGCTTCCCTCTGTCGAAAAATTTGTTTGCATGCTTGTTCATAGCCAACACCGGGAGCTTGCATTTTGGGTCCGCGGAGATGCTGTCTTGTCAGATGGATGTTTCTATCCTCTAAGCAGTGATTATAGCATTTCGAAGATTAAGGTTACTAATGGGTTTTATAGCCCCGAACCTGATGCTGTTCTTGAAACACATGATTTCAAAGCATATAAACTGGATTTCAGCCGGATGGCATTGAAGAGGGTACATGATTTGGGCGATCAAATACTATTTCTAGGATTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

157

Amino Acids

18.26

Weight (kDa)

9.17

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 32 - 89 5e-08 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27200
malus_domestica MD13G1035900.v1.1
prunus_persica Prupe.6G174300_v2.0.a1 Prupe.6G178100_v2.0.a1
pyrus_communis pycom13g03130 pycom16g03310
rosa_chinensis RchiOBHm_Chr4g0434481
rosa_laevigata RLG00000006668
rosa_multiflora Rmu_sc0002251.1_g000016 Rmu_sc0005899.1_g000009
rosa_roxburghii Rroxscaffold_5G00375830
rosa_rugosa Rorug04G0277000
rosa_samantha Rh4AG332600 Rh4BG340400 Rh4CG355600 Rh4DG335800
rosa_wichuraiana Rw4G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 153
AccII CGCG 1 cut(s) 258
AciI CCGC 2 cut(s) 256, 258
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 2 cut(s) 158, 203
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 429
AfeI AGCGCT 1 cut(s) 186
AfiI CCNNNNNNNGG 2 cut(s) 153, 235
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 5 cut(s) 115, 371, 385, 421, 466
AluBI AGCT 2 cut(s) 108, 241
AluI AGCT 2 cut(s) 108, 241
Alw26I GTCTC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 186
AoxI GGCC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 158, 203
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 1 cut(s) 253
AsuC2I CCSGG 1 cut(s) 236
AsuII TTCGAA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 253
BalI TGGCCA 1 cut(s) 12
BccI CCATC 4 cut(s) 72, 166, 272, 406
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 2 cut(s) 253, 349
Bpu14I TTCGAA 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 236
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 235
Bse1I ACTGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 287, 417
BseLI CCNNNNNNNGG 2 cut(s) 153, 235
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 235, 409
BslI CCNNNNNNNGG 2 cut(s) 153, 235
BsmAI GTCTC 1 cut(s) 12
BsnI GGCC 1 cut(s) 12
Bsp119I TTCGAA 1 cut(s) 317
Bsp143I GATC 2 cut(s) 139, 442
BspACI CCGC 2 cut(s) 256, 258
BspANI GGCC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 194
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 254
BspT104I TTCGAA 1 cut(s) 317
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 139, 442
BssNI GRCGYC 1 cut(s) 5
Bst6I CTCTTC 1 cut(s) 416
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 215, 243
BstDEI CTNAG 3 cut(s) 49, 181, 296
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 287, 417
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 142, 445
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 139, 442
BstNSI RCATGY 2 cut(s) 29, 217
BstSCI CCNGG 1 cut(s) 234
BstUI CGCG 1 cut(s) 258
BsuRI GGCC 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 2 cut(s) 287, 417
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 215, 243
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 253
Cfr42I CCGCGG 1 cut(s) 259
Csp6I GTAC 1 cut(s) 428
CviAII CATG 5 cut(s) 26, 153, 214, 377, 431
CviJI RGCY 6 cut(s) 12, 108, 228, 241, 348, 408
CviKI_1 RGCY 6 cut(s) 12, 108, 228, 241, 348, 408
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 3 cut(s) 49, 181, 296
DpnI GATC 2 cut(s) 141, 444
DpnII GATC 2 cut(s) 139, 442
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 253
Eco47III AGCGCT 1 cut(s) 186
FaeI CATG 5 cut(s) 29, 156, 217, 380, 434
FatI CATG 5 cut(s) 25, 152, 213, 376, 430
FokI GGATG 2 cut(s) 294, 424
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 1 cut(s) 12
HapII CCGG 2 cut(s) 235, 409
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 29, 156, 217, 380, 434
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 3 cut(s) 63, 115, 462
HpaII CCGG 2 cut(s) 235, 409
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 2 cut(s) 67, 368
Hpy8I GTNNAC 1 cut(s) 41
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 2 cut(s) 213, 245
HpyF3I CTNAG 3 cut(s) 49, 181, 296
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 29, 156, 217, 380, 434
HspAI GCGC 1 cut(s) 185
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 2 cut(s) 139, 442
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 4 cut(s) 248, 369, 383, 422
LweI GCATC 2 cut(s) 253, 349
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 89, 328
MalI GATC 2 cut(s) 141, 444
MboI GATC 2 cut(s) 139, 442
MboII GAAGA 4 cut(s) 134, 142, 331, 433
MlsI TGGCCA 1 cut(s) 12
MluCI AATT 2 cut(s) 158, 203
MluNI TGGCCA 1 cut(s) 12
MnlI CCTC 3 cut(s) 202, 303, 417
Mox20I TGGCCA 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 1 cut(s) 12
MseI TTAA 1 cut(s) 324
Msp20I TGGCCA 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 2 cut(s) 235, 409
MspR9I CCNGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 258
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 139, 442
NlaIII CATG 5 cut(s) 29, 156, 217, 380, 434
NlaIV GGNNCC 1 cut(s) 254
NspI RCATGY 2 cut(s) 29, 217
NspV TTCGAA 1 cut(s) 317
PaeI GCATGC 1 cut(s) 217
PciI ACATGT 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 63, 115, 462
PflMI CCANNNNNTGG 1 cut(s) 153
PscI ACATGT 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 253
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
SacII CCGCGG 1 cut(s) 259
SaqAI TTAA 1 cut(s) 324
Sau3AI GATC 2 cut(s) 139, 442
Sau96I GGNCC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 236
SetI ASST 6 cut(s) 8, 99, 110, 243, 330, 358
SfaNI GCATC 2 cut(s) 253, 349
Sfr303I CCGCGG 1 cut(s) 259
SfuI TTCGAA 1 cut(s) 317
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SphI GCATGC 1 cut(s) 217
Sse9I AATT 2 cut(s) 158, 203
SsiI CCGC 2 cut(s) 256, 258
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 234
TaiI ACGT 1 cut(s) 8
TaqI TCGA 3 cut(s) 84, 198, 317
TasI AATT 2 cut(s) 158, 203
TfiI GAWTC 3 cut(s) 63, 115, 462
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 253
XapI RAATTY 2 cut(s) 158, 203
XceI RCATGY 2 cut(s) 29, 217
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 458
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.