pycom16g04080

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014902
Physical Location & Seq
Reverse (-)
2502401 .. 2502999
599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGCACGTCATTTTCGTACTGCCTCTCCCAACGCCGTTACGGCTCCGTTACGCCGCAACCCTCAGCTCGACACGTTTCACTATGTTGGTCTGACCGGACTCAGCGTCGGTGGCGAGTTACTCCCGATTCCCGAGTCAACGTTCCAGATAGACGAGAGCGGAAACGGCGGAATCATCATCGACTCTGGCACGGCAGTGACTCGGCTGCAGACGGACACATACAGCGTGCTCCGCGACGCGTTCATGAAGGGCACCAAGGATTTGCCGTTTACGAAGGGGGCAGCGTTATTCGACGCGTGCTACGACTTGTCGTCGAGGAGGACCGTGGAGGTGCCAACAGTGTCCTTTCACTTCGCGGACGGAAAAGTGTTACCGTTACCGGCTAAAAATTTCCTGATACCGGTTGACTCGGACGGGACGTTTTGCTTTGCATTCGCGCCCACGCCGTCGGCGCTGTCAATCATTGGGAATGTCCAACAGCAAGGGACACGTGTCGGCTTCGACCTCGTGAATTCCGTTGTCGGGTTCTCCCGCAACCAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

182

Amino Acids

19.46

Weight (kDa)

6.26

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 28 - 177 3.1e-36 Xylanase inhibitor C-terminal
Asp PF00026 52 - 177 3.9e-06 Eukaryotic aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25510
fragaria_vesca FvH4_4g27770
malus_domestica MD13G1044900.v1.1
prunus_persica Prupe.1G307100_v2.0.a1
pyrus_communis pycom13g03970 pycom16g04080
rosa_chinensis RchiOBHm_Chr4g0435631
rosa_laevigata RLG00000006575
rosa_multiflora Rmu_sc0000051.1_g000004
rosa_roxburghii Rroxscaffold_5G00376850
rosa_rugosa Rorug04G0287400
rosa_samantha Rh4AG342600 Rh4BG350900 Rh4CG365600 Rh4DG345300
rosa_wichuraiana Rw4G029900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 251, 331
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 5 cut(s) 234, 239, 296, 356, 437
AciI CCGC 6 cut(s) 55, 159, 168, 232, 356, 532
AclI AACGTT 1 cut(s) 140
AcsI RAATTY 2 cut(s) 388, 511
AcvI CACGTG 1 cut(s) 491
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 400, 522
AflIII ACRYGT 5 cut(s) 72, 237, 294, 488, 490
AgeI ACCGGT 1 cut(s) 400
AhdI GACNNNNNGTC 2 cut(s) 104, 310
AjiI CACGTC 1 cut(s) 8
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw21I GWGCWC 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 131
ApeKI GCWGC 2 cut(s) 205, 281
ApoI RAATTY 2 cut(s) 388, 511
AsiGI ACCGGT 1 cut(s) 400
AspLEI GCGC 2 cut(s) 439, 454
AspS9I GGNCC 1 cut(s) 321
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 251, 331
BauI CACGAG 1 cut(s) 506
BbrPI CACGTG 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 192, 293
BceAI ACGGC 6 cut(s) 20, 57, 181, 207, 250, 430
BfmI CTRYAG 1 cut(s) 206
BfoI RGCGCY 1 cut(s) 455
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 55, 206, 282
BlsI GCNGC 3 cut(s) 56, 207, 283
Bme18I GGWCC 1 cut(s) 321
BmeRI GACNNNNNGTC 2 cut(s) 104, 310
BmeT110I CYCGRG 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 3 cut(s) 45, 253, 333
BoxI GACNNNNGTC 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 491
BsaJI CCNNGG 2 cut(s) 255, 324
BsaWI WCCGGW 2 cut(s) 95, 400
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 522
Bse118I RCCGGY 2 cut(s) 379, 400
BseDI CCNNGG 2 cut(s) 255, 324
BseLI CCNNNNNNNGG 2 cut(s) 400, 522
BseMII CTCAG 2 cut(s) 77, 115
BseRI GAGGAG 1 cut(s) 331
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 2 cut(s) 192, 293
Bsh1236I CGCG 5 cut(s) 234, 239, 296, 356, 437
BshNI GGYRCC 2 cut(s) 251, 331
BshTI ACCGGT 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 96, 380, 401
BslFI GGGAC 2 cut(s) 430, 499
BslI CCNNNNNNNGG 2 cut(s) 400, 522
BsmFI GGGAC 2 cut(s) 430, 499
BsmI GAATGC 1 cut(s) 431
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 231, 254
BspACI CCGC 6 cut(s) 55, 159, 168, 232, 356, 532
BspCNI CTCAG 2 cut(s) 76, 114
BspFNI CGCG 5 cut(s) 234, 239, 296, 356, 437
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 3 cut(s) 45, 253, 333
BspMAI CTGCAG 1 cut(s) 210
BspT107I GGYRCC 2 cut(s) 251, 331
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 379, 400
BssAI RCCGGY 2 cut(s) 379, 400
BssECI CCNNGG 2 cut(s) 255, 324
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 506
Bst4CI ACNGT 3 cut(s) 325, 340, 375
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 227, 298
BstDEI CTNAG 2 cut(s) 63, 101
BstDSI CCRYGG 1 cut(s) 324
BstFNI CGCG 5 cut(s) 234, 239, 296, 356, 437
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 439, 454
BstMWI GCNNNNNNNGC 5 cut(s) 41, 111, 165, 231, 451
BstPAI GACNNNNGTC 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 206
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 5 cut(s) 234, 239, 296, 356, 437
BstV1I GCAGC 2 cut(s) 192, 293
BtgI CCRYGG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 8
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 345
Cac8I GCNNGC 2 cut(s) 227, 298
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 439, 454
Cfr10I RCCGGY 2 cut(s) 379, 400
Cfr13I GGNCC 1 cut(s) 321
CseI GACGC 3 cut(s) 94, 245, 302
Csp6I GTAC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 400
CviAII CATG 1 cut(s) 244
CviJI RGCY 5 cut(s) 44, 67, 205, 383, 498
CviKI_1 RGCY 5 cut(s) 44, 67, 205, 383, 498
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 2 cut(s) 63, 101
DriI GACNNNNNGTC 2 cut(s) 104, 310
Eam1105I GACNNNNNGTC 2 cut(s) 104, 310
EciI GGCGGA 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 321
Eco72I CACGTG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 1 cut(s) 247
FaiI YATR 3 cut(s) 84, 220, 245
FaqI GGGAC 2 cut(s) 430, 499
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 539
Fnu4HI GCNGC 3 cut(s) 55, 206, 282
Fsp4HI GCNGC 3 cut(s) 55, 206, 282
GlaI GCGC 2 cut(s) 438, 453
GluI GCNGC 3 cut(s) 55, 206, 282
HaeII RGCGCY 1 cut(s) 455
HapII CCGG 3 cut(s) 96, 380, 401
HgaI GACGC 3 cut(s) 94, 245, 302
HhaI GCGC 2 cut(s) 439, 454
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 2 cut(s) 437, 452
HinP1I GCGC 2 cut(s) 437, 452
HincII GTYRAC 2 cut(s) 138, 406
HindII GTYRAC 2 cut(s) 138, 406
HinfI GANTC 7 cut(s) 99, 127, 134, 171, 182, 199, 407
HpaII CCGG 3 cut(s) 96, 380, 401
Hpy166II GTNNAC 3 cut(s) 138, 270, 406
Hpy188I TCNGA 2 cut(s) 93, 412
Hpy188III TCNNGA 6 cut(s) 124, 131, 145, 244, 394, 508
Hpy8I GTNNAC 3 cut(s) 138, 270, 406
Hpy99I CGWCG 5 cut(s) 110, 239, 296, 316, 451
HpyAV CCTTC 2 cut(s) 241, 268
HpyCH4III ACNGT 3 cut(s) 325, 340, 375
HpyCH4IV ACGT 5 cut(s) 7, 74, 140, 419, 490
HpyCH4V TGCA 2 cut(s) 208, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 111, 165, 231, 451
HpyF3I CTNAG 2 cut(s) 63, 101
HpySE526I ACGT 5 cut(s) 7, 74, 140, 419, 490
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 2 cut(s) 437, 452
LmnI GCTCC 2 cut(s) 49, 234
LpnPI CCDG 6 cut(s) 109, 158, 171, 393, 407, 414
Lsp1109I GCAGC 2 cut(s) 192, 293
MaeII ACGT 5 cut(s) 7, 74, 140, 419, 490
MaeIII GTNAC 6 cut(s) 37, 48, 117, 196, 369, 375
MbiI CCGCTC 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 231, 254
MluCI AATT 2 cut(s) 388, 511
MluI ACGCGT 2 cut(s) 237, 294
MlyI GAGTC 5 cut(s) 93, 143, 176, 193, 401
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 6 cut(s) 34, 72, 309, 312, 322, 515
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 3 cut(s) 96, 380, 401
Mva1269I GAATGC 1 cut(s) 431
MvnI CGCG 5 cut(s) 234, 239, 296, 356, 437
MwoI GCNNNNNNNGC 5 cut(s) 41, 111, 165, 231, 451
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 45, 253, 333
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 194
PagI TCATGA 1 cut(s) 243
PcsI WCGNNNNNNNCGW 4 cut(s) 13, 300, 416, 513
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 127, 171
PinAI ACCGGT 1 cut(s) 400
PkrI GCNGC 3 cut(s) 56, 207, 283
PleI GAGTC 5 cut(s) 93, 142, 176, 193, 401
PmaCI CACGTG 1 cut(s) 491
PmlI CACGTG 1 cut(s) 491
PpsI GAGTC 5 cut(s) 93, 142, 176, 193, 401
Ppu21I YACGTR 1 cut(s) 491
PshAI GACNNNNGTC 1 cut(s) 491
Psp1406I AACGTT 1 cut(s) 140
PspCI CACGTG 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 45, 253, 333
PspPI GGNCC 1 cut(s) 321
PstI CTGCAG 1 cut(s) 210
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
RseI CAYNNNNRTG 1 cut(s) 194
SatI GCNGC 3 cut(s) 55, 206, 282
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 5 cut(s) 93, 143, 176, 193, 401
SduI GDGCHC 2 cut(s) 231, 254
SetI ASST 8 cut(s) 10, 69, 77, 143, 333, 422, 493, 507
SfcI CTRYAG 1 cut(s) 206
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 2 cut(s) 388, 511
SsiI CCGC 6 cut(s) 55, 159, 168, 232, 356, 532
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 3 cut(s) 325, 340, 375
TaiI ACGT 5 cut(s) 10, 77, 143, 422, 493
TaqI TCGA 5 cut(s) 69, 180, 291, 314, 501
TasI AATT 2 cut(s) 388, 511
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 2 cut(s) 127, 171
TscAI CASTG 2 cut(s) 201, 345
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 205, 281
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 232, 260
TspGWI ACGGA 4 cut(s) 36, 227, 375, 505
TspRI CASTG 2 cut(s) 201, 345
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 2 cut(s) 388, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.