pycom16g05050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014221
Physical Location & Seq
Forward (+)
3254804 .. 3255617
814 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGAAAGAGAGAGGCAAAGCTGTGGAAACAGCTCACGACAATATCGACATGGAGTACTACTCTTCGTCCGAAATGCCGTGTCGAAGACACCCAAATTCGTCTTCCGTGGGGATATGCGCATTCTGTCTAAAAGATCGTCTACTCCAGCTGGTCTGCTCCGACTGTGGCGAGCAGCGCCTCTCCTCCTGCTCCTGCTCCGCCGTCTCGTCAAACCGCAACTCCACCGTGGAGGTTGGCAGCGTCGGCAGAGTCTCGTTTCTGATCGAGAACGAAAAGCACGAGTCTTTACAAAAACCCAACAAAACCCAAGAGAAAGAAGACGTCGTGGTGCTCCGGAGAAGCAGCAGCAGCTGCGTCGAGATCAAGAAAAGTAATTTTTGGAGATTTGGGAGGTTTTTTCGGAAGAAGAGATCGGAAAAAGATGGTGGGAATACGAGTGTTGGTGGGTTTGATGAGAAAAGTGAGATGTGGCTGGTGGATCATATGGGGGTGTCGAGATCAAGGTCTCTCTGCAGTTTCAGAGGTGGGGCACTCTTAGGATCGGAGGACGGCGGGGAACATTTGATGGTTTCCGCCGCCAGGAGCTCCATTTCTGCTGCGAGAAGCTCGAGCGTTACTACTGCCGGAATGGTTCTGGATTCTGGAAGAAAGAGTGGATTTAGTGAAGGGAGAAAGAGTGGATTTATGGATTCAGTGAAGGGAGAAGGAGCGGATTTAGTGAAGCGGAGCCGAGAAAAAGCGGTTTCGATGGTGAGAAGAAAGATGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

28.27

Weight (kDa)

9.41

Isoelectric Point (pI)

61.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25590
fragaria_vesca FvH4_4g28580
malus_domestica MD13G1052200.v1.1 MD16G1057700.v1.1
prunus_persica Prupe.1G299900_v2.0.a1
pyrus_communis pycom13g04650 pycom16g05050
rosa_chinensis RchiOBHm_Chr4g0436671
rosa_laevigata RLG00000006505
rosa_multiflora Rmu_sc0003130.1_g000019
rosa_roxburghii Rroxscaffold_5G00377750
rosa_rugosa Rorug04G0295100
rosa_samantha Rh4AG349600 Rh4BG358300 Rh4CG372700 Rh4DG352500
rosa_wichuraiana Rw4G030580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
Acc16I TGCGCA 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 710
AccI GTMKAC 1 cut(s) 139
AccIII TCCGGA 1 cut(s) 333
AciI CCGC 8 cut(s) 198, 214, 552, 573, 576, 710, 724, 740
AclWI GGATC 2 cut(s) 486, 547
AcsI RAATTY 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 579
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 6 cut(s) 20, 32, 148, 351, 585, 606
AluI AGCT 6 cut(s) 20, 32, 148, 351, 585, 606
Alw21I GWGCWC 2 cut(s) 333, 587
Alw26I GTCTC 3 cut(s) 208, 256, 510
AlwI GGATC 2 cut(s) 486, 547
AlwNI CAGNNNCTG 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 607
Aor13HI TCCGGA 1 cut(s) 333
ApeKI GCWGC 7 cut(s) 172, 237, 342, 345, 348, 351, 596
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 2 cut(s) 119, 177
AsuHPI GGTGA 1 cut(s) 763
AvaI CYCGRG 1 cut(s) 607
BaeGI GKGCMC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 3 cut(s) 91, 93, 324
Bbv12I GWGCWC 2 cut(s) 333, 587
BbvI GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BccI CCATC 3 cut(s) 416, 559, 742
BceAI ACGGC 3 cut(s) 61, 185, 565
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 1 cut(s) 580
BcoDI GTCTC 3 cut(s) 208, 256, 510
BfmI CTRYAG 1 cut(s) 511
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 8 cut(s) 173, 238, 343, 346, 349, 352, 576, 597
BlsI GCNGC 8 cut(s) 174, 239, 344, 347, 350, 353, 577, 598
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 607
BmiI GGNNCC 1 cut(s) 728
BmrFI CCNGG 1 cut(s) 580
BpiI GAAGAC 3 cut(s) 91, 93, 324
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 105, 225
BsaWI WCCGGW 1 cut(s) 333
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 661, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 579
BseAI TCCGGA 1 cut(s) 333
BseBI CCWGG 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 105, 225
BseLI CCNNNNNNNGG 1 cut(s) 579
BseRI GAGGAG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BsiHKAI GWGCWC 2 cut(s) 333, 587
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 2 cut(s) 334, 624
BslI CCNNNNNNNGG 1 cut(s) 579
BsmAI GTCTC 3 cut(s) 208, 256, 510
BsmBI CGTCTC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 510
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 3 cut(s) 333, 532, 587
Bsp13I TCCGGA 1 cut(s) 333
Bsp143I GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
BspACI CCGC 8 cut(s) 198, 214, 552, 573, 576, 710, 724, 740
BspEI TCCGGA 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 728
BspMAI CTGCAG 1 cut(s) 515
BspPI GGATC 2 cut(s) 486, 547
BspTNI GGTCTC 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 710
BssECI CCNNGG 2 cut(s) 105, 225
BssMI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 2 cut(s) 164, 226
Bst6I CTCTTC 2 cut(s) 67, 401
BstACI GRCGYC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 1 cut(s) 535
BstDSI CCRYGG 2 cut(s) 105, 225
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 119, 177
BstKTI GATC 7 cut(s) 136, 264, 363, 413, 481, 500, 542
BstMAI GTCTC 3 cut(s) 208, 256, 510
BstMBI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
BstMWI GCNNNNNNNGC 4 cut(s) 174, 243, 348, 351
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 532
BstV1I GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
BstV2I GAAGAC 3 cut(s) 91, 93, 324
BtgI CCRYGG 2 cut(s) 105, 225
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 170
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 2 cut(s) 119, 177
CseI GACGC 2 cut(s) 229, 343
Csp6I GTAC 1 cut(s) 55
CviAII CATG 1 cut(s) 49
CviJI RGCY 8 cut(s) 20, 32, 148, 351, 472, 585, 606, 729
CviKI_1 RGCY 8 cut(s) 20, 32, 148, 351, 472, 585, 606, 729
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 535
DpnI GATC 7 cut(s) 135, 263, 362, 412, 480, 499, 541
DpnII GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
Eam1104I CTCTTC 2 cut(s) 67, 401
EarI CTCTTC 2 cut(s) 67, 401
EciI GGCGGA 2 cut(s) 187, 562
Ecl136II GAGCTC 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 587
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 1 cut(s) 52
FaiI YATR 5 cut(s) 50, 115, 483, 485, 686
FatI CATG 1 cut(s) 48
FauI CCCGC 1 cut(s) 545
FauNDI CATATG 1 cut(s) 483
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 8 cut(s) 173, 238, 343, 346, 349, 352, 576, 597
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 8 cut(s) 173, 238, 343, 346, 349, 352, 576, 597
FspAI RTGCGCAY 1 cut(s) 118
FspI TGCGCA 1 cut(s) 118
GlaI GCGC 2 cut(s) 118, 176
GluI GCNGC 8 cut(s) 173, 238, 343, 346, 349, 352, 576, 597
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 178
HapII CCGG 2 cut(s) 334, 624
HgaI GACGC 2 cut(s) 229, 343
HhaI GCGC 2 cut(s) 119, 177
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 2 cut(s) 117, 175
HinP1I GCGC 2 cut(s) 117, 175
HinfI GANTC 4 cut(s) 249, 281, 638, 689
HpaII CCGG 2 cut(s) 334, 624
HphI GGTGA 1 cut(s) 763
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 7 cut(s) 70, 160, 261, 402, 415, 521, 544
Hpy188III TCNNGA 8 cut(s) 35, 265, 334, 358, 364, 495, 635, 642
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 3 cut(s) 245, 326, 359
HpyAV CCTTC 3 cut(s) 659, 691, 698
HpyCH4III ACNGT 2 cut(s) 164, 226
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 243, 348, 351
HpyF3I CTNAG 1 cut(s) 535
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 2 cut(s) 117, 175
Kpn2I TCCGGA 1 cut(s) 333
Kzo9I GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
LmnI GCTCC 8 cut(s) 161, 194, 200, 336, 582, 590, 707, 726
Lsp1109I GCAGC 7 cut(s) 184, 249, 338, 354, 357, 360, 583
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 613
MalI GATC 7 cut(s) 135, 263, 362, 412, 480, 499, 541
MbiI CCGCTC 1 cut(s) 710
MboI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
MboII GAAGA 8 cut(s) 54, 93, 96, 329, 415, 418, 657, 768
MhlI GDGCHC 3 cut(s) 333, 532, 587
MluCI AATT 2 cut(s) 94, 373
MlyI GAGTC 2 cut(s) 258, 290
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 7 cut(s) 5, 188, 193, 223, 384, 515, 538
MroI TCCGGA 1 cut(s) 333
MspA1I CMGCKG 2 cut(s) 148, 351
MspI CCGG 2 cut(s) 334, 624
MspR9I CCNGG 1 cut(s) 580
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 1 cut(s) 580
MwoI GCNNNNNNNGC 4 cut(s) 174, 243, 348, 351
NdeI CATATG 1 cut(s) 483
NdeII GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 728
NmeAIII GCCGAG 1 cut(s) 755
NsbI TGCGCA 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 607
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 276
PctI GAATGC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 638, 689
PkrI GCNGC 8 cut(s) 174, 239, 344, 347, 350, 353, 577, 598
PleI GAGTC 2 cut(s) 257, 289
PpsI GAGTC 2 cut(s) 257, 289
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 728
PspXI VCTCGAGB 1 cut(s) 607
PstI CTGCAG 1 cut(s) 515
PstNI CAGNNNCTG 1 cut(s) 351
PvuII CAGCTG 2 cut(s) 148, 351
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 587
SatI GCNGC 8 cut(s) 173, 238, 343, 346, 349, 352, 576, 597
Sau3AI GATC 7 cut(s) 133, 261, 360, 410, 478, 497, 539
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 2 cut(s) 258, 290
ScrFI CCNGG 1 cut(s) 580
SduI GDGCHC 3 cut(s) 333, 532, 587
SfcI CTRYAG 1 cut(s) 511
Sfr274I CTCGAG 1 cut(s) 607
SlaI CTCGAG 1 cut(s) 607
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 2 cut(s) 94, 373
SsiI CCGC 8 cut(s) 198, 214, 552, 573, 576, 710, 724, 740
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 578
TaaI ACNGT 2 cut(s) 164, 226
TaiI ACGT 1 cut(s) 324
TaqI TCGA 7 cut(s) 45, 82, 264, 357, 494, 608, 746
TasI AATT 2 cut(s) 94, 373
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 2 cut(s) 638, 689
TscAI CASTG 1 cut(s) 699
TseI GCWGC 7 cut(s) 172, 237, 342, 345, 348, 351, 596
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 699
XapI RAATTY 1 cut(s) 94
XhoI CTCGAG 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 139
ZraI GACGTC 1 cut(s) 322
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.