pycom16g05560

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011334
Physical Location & Seq
Forward (+)
3591217 .. 3594915
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1149 bp
ATGCATGTGGACCGGGTCACCGACGGGAGGAAGAAGGCAGAGTTCCAATCTCTGGAGAGGAGAGATCGAAGAAGCTCAAGTGAAATCACTAATCTCTCAGAAGTCCGAGATCAGAGATCTGCTGCGAGATATCTCCAAAATCCCCTTTTGGAGACGACAATGGTGGTTCGTCTTCCAATTGCCGCGTTTTCTCTGGTCCTCCTCCTCGTCTTCCCTCCGCTGCTTCTCGACGCCAAAACCCTCAAACGCGACATGAAAGCACTGAACGAGATCAAGGCTTCGCTGGGGTGGAGAGTGGTGTACGCCTGGGTAGGCGACGATCCGTGCGGAGACGGCGACCTTCCCCCGTGGTCCGGTGTCACTTGCTCCACTCAAGGCGATTACCGAGTCGTCACCGAGTTGGAGGTGTATGCAGTGTCAATTGTTGGGCCTTTCCCTACTGCTGTCACCAATCTCTTGGATCTCACTAGGCTGGATCTCCATAACAACAAGTTAACCGGCCCTATTCCTCCGCAAATCGGACGTTTGAAGCGTCTCAAAATGCTTAACTTGAGATGGAATAAGCTACAAGATGTCATTCCTCCTGAAATTGGTGGATTGAAGAGTTTAACCCATTTGCATCTAAGCTTTAATAGTTTTAAAGGAGAAATCCCGAAGGAGCTTGCTAATCTTCCTGAGCTTCGCTATCTGTATCTGCAAGAAAATCGCCTTGTTGGGCGGATTCCTCCAGAACTAGGAACTCTGCAAAATCTTCGGCATTTGGACGTTGGTAACAATCATTTGGTGGGTACTATTAGGGAACTCATACGTATTGAGGGCTGCTTTCCCGCTTTGCGTAACCTTTACCTAAACAACAACTATCTTACCGGAGGAATTCCAGCACAGCTCGCAAGCTTGTCCAAACTGGAAATACTGTACCTATCTTACAACAAGATGTCTGGGATAGTTCCATTAGGACTTTCCCATATTCCGAGACTGACGTACTTGTACTTGGATCACAATCAATTTTCAGGAAGAATTCCTGATGCCTTCTACAAACACCCGTTCTTGAAAGACATGTATATTGAAGGGAATGCATTCCGACCAGGTGTAAAACCAATAGGCATACACAAAGTTCTCGAGCTCACAGACGCAGAATTTCTCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8

Protein Analysis

383

Amino Acids

43.21

Weight (kDa)

9.16

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 149 - 255 2.9e-10 Leucine-rich repeat region
LRR_8 PF13855 198 - 237 8.9e-07 Leucine rich repeat
LRR_4 PF12799 225 - 268 1.5e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 276 - 336 2.8e-13 Leucine rich repeat
LRR_4 PF12799 276 - 311 5.2e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61240 AT5G61240
fragaria_vesca FvH4_4g30420
malus_domestica MD13G1065200.v1.1 MD16G1063500.v1.1
prunus_persica Prupe.1G283000_v2.0.a1 Prupe.1G283000_v2.0.a1
pyrus_communis pycom13g05760 pycom16g05560
rosa_chinensis RchiOBHm_Chr4g0438711
rosa_laevigata RLG00000006335
rosa_multiflora Rmu_sc0002505.1_g000005
rosa_roxburghii Rroxscaffold_5G00379750
rosa_rugosa Rorug04G0312700
rosa_samantha Rh4AG363300 Rh4BG374700 Rh4CG388900 Rh4DG369200
rosa_wichuraiana Rw0G007180 Rw0G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 52
AccII CGCG 2 cut(s) 185, 249
AciI CCGC 6 cut(s) 183, 218, 327, 512, 718, 828
AclWI GGATC 4 cut(s) 314, 468, 483, 1002
AcsI RAATTY 3 cut(s) 873, 1017, 1136
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 5 cut(s) 302, 790, 917, 983, 989
AfiI CCNNNNNNNGG 6 cut(s) 27, 52, 353, 518, 590, 734
AflIII ACRYGT 1 cut(s) 1056
AgsI TTSAA 4 cut(s) 529, 601, 1051, 1067
AjnI CCWGG 2 cut(s) 305, 1084
AluBI AGCT 8 cut(s) 75, 565, 627, 661, 679, 886, 894, 1123
AluI AGCT 8 cut(s) 75, 565, 627, 661, 679, 886, 894, 1123
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 4 cut(s) 146, 324, 539, 967
AlwI GGATC 4 cut(s) 314, 468, 483, 1002
Ama87I CYCGRG 1 cut(s) 1118
AoxI GGCC 2 cut(s) 428, 499
ApeKI GCWGC 3 cut(s) 122, 220, 819
ApoI RAATTY 3 cut(s) 873, 1017, 1136
Asp700I GAANNNNTTC 1 cut(s) 1076
AspS9I GGNCC 5 cut(s) 10, 196, 351, 428, 500
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 3 cut(s) 10, 385, 439
AvaI CYCGRG 1 cut(s) 1118
AvaII GGWCC 3 cut(s) 10, 196, 351
BanII GRGCYC 1 cut(s) 1125
BbsI GAAGAC 2 cut(s) 164, 202
Bbv12I GWGCWC 1 cut(s) 1125
BbvI GCAGC 3 cut(s) 109, 207, 806
BccI CCATC 1 cut(s) 549
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 4 cut(s) 686, 720, 734, 768
BciT130I CCWGG 2 cut(s) 307, 1086
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 4 cut(s) 146, 324, 539, 967
BfaI CTAG 3 cut(s) 468, 734, 1147
BglII AGATCT 1 cut(s) 116
BisI GCNGC 4 cut(s) 123, 183, 221, 820
BlsI GCNGC 4 cut(s) 124, 184, 222, 821
Bme1390I CCNGG 3 cut(s) 14, 307, 1086
Bme18I GGWCC 3 cut(s) 10, 196, 351
BmeT110I CYCGRG 1 cut(s) 1118
BmgT120I GGNCC 5 cut(s) 10, 196, 351, 428, 500
BmrFI CCNGG 3 cut(s) 14, 307, 1086
BmsI GCATC 2 cut(s) 628, 1015
BpiI GAAGAC 2 cut(s) 164, 202
BpmI CTGGAG 2 cut(s) 74, 711
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 3 cut(s) 61, 357, 571
BpuMI CCSGG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 999
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 306, 347
BsaWI WCCGGW 2 cut(s) 353, 866
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 52, 353, 518, 590, 734
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 909
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 2 cut(s) 307, 1086
BseDI CCNNGG 2 cut(s) 306, 347
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 6 cut(s) 27, 52, 353, 518, 590, 734
BseMII CTCAG 2 cut(s) 111, 666
BseNI ACTGG 1 cut(s) 909
BseRI GAGGAG 3 cut(s) 73, 191, 194
BseXI GCAGC 3 cut(s) 109, 207, 806
BseYI CCCAGC 1 cut(s) 283
Bsh1236I CGCG 2 cut(s) 185, 249
BshFI GGCC 2 cut(s) 430, 501
BsiHKAI GWGCWC 1 cut(s) 1125
BsiHKCI CYCGRG 1 cut(s) 1118
BsiSI CCGG 4 cut(s) 13, 354, 498, 867
BslI CCNNNNNNNGG 6 cut(s) 27, 52, 353, 518, 590, 734
BsmAI GTCTC 4 cut(s) 146, 324, 539, 967
BsmBI CGTCTC 3 cut(s) 146, 324, 539
BsmI GAATGC 2 cut(s) 1076, 1078
BsnI GGCC 2 cut(s) 430, 501
BsoBI CYCGRG 1 cut(s) 1118
Bsp1286I GDGCHC 1 cut(s) 1125
Bsp143I GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
BspACI CCGC 6 cut(s) 183, 218, 327, 512, 718, 828
BspANI GGCC 2 cut(s) 430, 501
BspCNI CTCAG 2 cut(s) 110, 667
BspFNI CGCG 2 cut(s) 185, 249
BspPI GGATC 4 cut(s) 314, 468, 483, 1002
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 1 cut(s) 909
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 306, 347
BssMI GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 2 cut(s) 307, 1086
Bst4CI ACNGT 1 cut(s) 915
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 809
BstC8I GCNNGC 3 cut(s) 663, 888, 892
BstDEI CTNAG 3 cut(s) 97, 623, 675
BstDSI CCRYGG 1 cut(s) 347
BstEII GGTNACC 1 cut(s) 16
BstFNI CGCG 2 cut(s) 185, 249
BstKTI GATC 8 cut(s) 67, 112, 119, 273, 322, 463, 478, 997
BstMAI GTCTC 4 cut(s) 146, 324, 539, 967
BstMBI GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
BstMWI GCNNNNNNNGC 2 cut(s) 333, 887
BstNI CCWGG 2 cut(s) 307, 1086
BstNSI RCATGY 2 cut(s) 8, 1060
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 3 cut(s) 12, 305, 1084
BstSNI TACGTA 1 cut(s) 809
BstUI CGCG 2 cut(s) 185, 249
BstV1I GCAGC 3 cut(s) 109, 207, 806
BstV2I GAAGAC 2 cut(s) 164, 202
BstX2I RGATCY 3 cut(s) 116, 460, 475
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 3 cut(s) 116, 460, 475
BsuRI GGCC 2 cut(s) 430, 501
BtgI CCRYGG 1 cut(s) 347
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 2 cut(s) 260, 420
Cac8I GCNNGC 3 cut(s) 663, 888, 892
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 5 cut(s) 10, 196, 351, 428, 500
CseI GACGC 3 cut(s) 239, 521, 1139
CsiI ACCWGGT 1 cut(s) 1084
Csp6I GTAC 5 cut(s) 301, 789, 916, 982, 988
CviAII CATG 3 cut(s) 5, 253, 1057
CviQI GTAC 5 cut(s) 301, 789, 916, 982, 988
DdeI CTNAG 3 cut(s) 97, 623, 675
DpnI GATC 8 cut(s) 66, 111, 118, 272, 321, 462, 477, 996
DpnII GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
DraI TTTAAA 1 cut(s) 640
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 1 cut(s) 733
Ecl136II GAGCTC 1 cut(s) 1123
Eco105I TACGTA 1 cut(s) 809
Eco24I GRGCYC 1 cut(s) 1125
Eco32I GATATC 1 cut(s) 131
Eco47I GGWCC 3 cut(s) 10, 196, 351
Eco53kI GAGCTC 1 cut(s) 1123
Eco88I CYCGRG 1 cut(s) 1118
Eco91I GGTNACC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 1123
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 2 cut(s) 873, 1017
EcoRII CCWGG 2 cut(s) 305, 1084
EcoRV GATATC 1 cut(s) 131
EcoT22I ATGCAT 2 cut(s) 6, 1078
EcoT38I GRGCYC 1 cut(s) 1125
Esp3I CGTCTC 3 cut(s) 146, 324, 539
FaeI CATG 3 cut(s) 8, 256, 1060
FaiI YATR 9 cut(s) 6, 254, 411, 483, 806, 966, 1058, 1062, 1106
FatI CATG 3 cut(s) 4, 252, 1056
FauI CCCGC 1 cut(s) 835
Fnu4HI GCNGC 4 cut(s) 123, 183, 221, 820
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 4 cut(s) 123, 183, 221, 820
FspBI CTAG 3 cut(s) 468, 734, 1147
GluI GCNGC 4 cut(s) 123, 183, 221, 820
GsaI CCCAGC 1 cut(s) 287
GsuI CTGGAG 2 cut(s) 74, 711
HaeIII GGCC 2 cut(s) 430, 501
HapII CCGG 4 cut(s) 13, 354, 498, 867
HgaI GACGC 3 cut(s) 239, 521, 1139
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 3 cut(s) 8, 256, 1060
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 2 cut(s) 625, 892
HinfI GANTC 2 cut(s) 387, 721
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 4 cut(s) 13, 354, 498, 867
HphI GGTGA 3 cut(s) 10, 385, 439
Hpy166II GTNNAC 3 cut(s) 10, 301, 495
Hpy188I TCNGA 6 cut(s) 100, 107, 114, 521, 972, 1082
Hpy8I GTNNAC 3 cut(s) 10, 301, 495
Hpy99I CGWCG 3 cut(s) 26, 233, 320
HpyAV CCTTC 5 cut(s) 28, 350, 649, 1039, 1061
HpyCH4III ACNGT 1 cut(s) 915
HpyCH4IV ACGT 4 cut(s) 523, 765, 808, 980
HpyCH4V TGCA 6 cut(s) 4, 413, 619, 697, 745, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 887
HpyF3I CTNAG 3 cut(s) 97, 623, 675
HpySE526I ACGT 4 cut(s) 523, 765, 808, 980
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 3 cut(s) 8, 256, 1060
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
LmnI GCTCC 2 cut(s) 371, 658
Lsp1109I GCAGC 3 cut(s) 109, 207, 806
LweI GCATC 2 cut(s) 628, 1015
MabI ACCWGGT 1 cut(s) 1084
MaeI CTAG 3 cut(s) 468, 734, 1147
MaeII ACGT 4 cut(s) 523, 765, 808, 980
MaeIII GTNAC 6 cut(s) 16, 358, 391, 445, 770, 836
MalI GATC 8 cut(s) 66, 111, 118, 272, 321, 462, 477, 996
MboI GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
MboII GAAGA 8 cut(s) 43, 81, 164, 202, 613, 662, 743, 1026
MfeI CAATTG 2 cut(s) 177, 420
MflI RGATCY 3 cut(s) 116, 460, 475
MhlI GDGCHC 1 cut(s) 1125
MluCI AATT 7 cut(s) 177, 420, 588, 873, 1004, 1017, 1136
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 2 cut(s) 381, 1105
Mph1103I ATGCAT 2 cut(s) 6, 1078
MroXI GAANNNNTTC 1 cut(s) 1076
MseI TTAA 5 cut(s) 494, 546, 608, 630, 639
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 4 cut(s) 13, 354, 498, 867
MspR9I CCNGG 3 cut(s) 14, 307, 1086
MunI CAATTG 2 cut(s) 177, 420
Mva1269I GAATGC 2 cut(s) 1076, 1078
MvaI CCWGG 2 cut(s) 307, 1086
MvnI CGCG 2 cut(s) 185, 249
MwoI GCNNNNNNNGC 2 cut(s) 333, 887
NciI CCSGG 1 cut(s) 14
NdeII GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
NlaIII CATG 3 cut(s) 8, 256, 1060
NmuCI GTSAC 4 cut(s) 16, 358, 391, 445
NsiI ATGCAT 2 cut(s) 6, 1078
NspI RCATGY 2 cut(s) 8, 1060
PaeR7I CTCGAG 1 cut(s) 1118
PciI ACATGT 1 cut(s) 1056
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PctI GAATGC 2 cut(s) 1076, 1078
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 1 cut(s) 721
PflFI GACNNNGTC 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 52
PkrI GCNGC 4 cut(s) 124, 184, 222, 821
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 809
PscI ACATGT 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 1125
Psp6I CCWGG 2 cut(s) 305, 1084
PspEI GGTNACC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 283
PspGI CCWGG 2 cut(s) 305, 1084
PspPI GGNCC 5 cut(s) 10, 196, 351, 428, 500
PsuI RGATCY 3 cut(s) 116, 460, 475
PsyI GACNNNGTC 1 cut(s) 14
RsaI GTAC 5 cut(s) 302, 790, 917, 983, 989
RsaNI GTAC 5 cut(s) 301, 789, 916, 982, 988
SacI GAGCTC 1 cut(s) 1125
SaqAI TTAA 5 cut(s) 494, 546, 608, 630, 639
SatI GCNGC 4 cut(s) 123, 183, 221, 820
Sau3AI GATC 8 cut(s) 64, 109, 116, 270, 319, 460, 475, 994
Sau96I GGNCC 5 cut(s) 10, 196, 351, 428, 500
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 3 cut(s) 14, 307, 1086
SduI GDGCHC 1 cut(s) 1125
SexAI ACCWGGT 1 cut(s) 1084
SfaNI GCATC 2 cut(s) 628, 1015
Sfr274I CTCGAG 1 cut(s) 1118
SinI GGWCC 3 cut(s) 10, 196, 351
SlaI CTCGAG 1 cut(s) 1118
SmlI CTYRAG 4 cut(s) 76, 372, 550, 1118
SmoI CTYRAG 4 cut(s) 76, 372, 550, 1118
SnaBI TACGTA 1 cut(s) 809
Sse9I AATT 7 cut(s) 177, 420, 588, 873, 1004, 1017, 1136
SsiI CCGC 6 cut(s) 183, 218, 327, 512, 718, 828
SspMI CTAG 3 cut(s) 468, 734, 1147
SstI GAGCTC 1 cut(s) 1125
StyD4I CCNGG 3 cut(s) 12, 305, 1084
TaaI ACNGT 1 cut(s) 915
TaiI ACGT 4 cut(s) 526, 768, 811, 983
TaqI TCGA 3 cut(s) 67, 228, 1119
TasI AATT 7 cut(s) 177, 420, 588, 873, 1004, 1017, 1136
TatI WGTACW 1 cut(s) 987
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 1 cut(s) 721
Tru1I TTAA 5 cut(s) 494, 546, 608, 630, 639
Tru9I TTAA 5 cut(s) 494, 546, 608, 630, 639
TscAI CASTG 2 cut(s) 267, 420
TseFI GTSAC 4 cut(s) 16, 358, 391, 445
TseI GCWGC 3 cut(s) 122, 220, 819
Tsp45I GTSAC 4 cut(s) 16, 358, 391, 445
TspDTI ATGAA 1 cut(s) 269
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 2 cut(s) 267, 420
Tth111I GACNNNGTC 1 cut(s) 14
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 3 cut(s) 10, 196, 351
XapI RAATTY 3 cut(s) 873, 1017, 1136
XceI RCATGY 2 cut(s) 8, 1060
XhoI CTCGAG 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 3 cut(s) 468, 734, 1147
Zsp2I ATGCAT 2 cut(s) 6, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.