pycom16g05700

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007903
Physical Location & Seq
Reverse (-)
3646996 .. 3650776
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3168 bp
ATGCCCGGGTATTACTCAGTGAGGGATCTTAACGAGGATCCAAACAATTGTAGCTGGCCCTTATATTACGGAGATAAAACCTTGTCAAATAGGCAGTATCGCAATGGTTTCTTGCCGAGGGCCATGCCTGATGCTTTTCCAGGGTATGATAAGGATGTAGTCAAGCAGACCATGCTTAAGCATGAGGCCATATTTAAGAATCAGGTGATTGAACTTCACCGGCTGTACAGAATTCAAAGGGACTTGATGGATGAAATAAAATGGAAAGAACTACATAGAAACCAGATACCCATGGAGACATCATTGTTGTCAAGTCCCTTAGCATCTCAAATTACATCTGAAGATGCTCGGAAATGGCATGATTCCAGCCTTCCTTTGGTAAACTCTGTTAATGCTGGACCATCGATTCCAGGTGTTGAAGGTACCCATTCTCCATCTAGTGCCGTGAAAGGAAACAACCAAAAAATTGGTTTGTTTCCATACCAAAATGGGATTAGTTCGAAAGTTGTTGAGGTGATGGAGTCAAGACCCACAAAAGTGAGGAAAAAAATGTTTGATCTTCAACTTCCAGCTGATGTGTATGTTGATTCTGACGAAGGGGAGCAGTTCACTGATGAGAAAGCTTCTGGCACTCCTACTTGTCAGCCGAATAAAAATTGTAAAACCACACTCGAGGGTGGTGCAAAGCTCTTTTTCTGCGAAGGTGCGAAGACAGATTGCAAAGGACATGTTTCAAGATCGATTGATACATGTTCGAGGAGCACAAATGGTTTGGCTGACTTGAATGAGCCTATTCAAATTGAAGAAACAAATGCTTCAGAATACGCTTATCCTCTGGGCCATGACTCTTATCATGGAAAGATTCAGGGTACCGATCAGGCGGCTAAGTCTAGATTGCAGCTTTTAGGTTTGCCGAAGGAAATATCACTCAACCCTCATCATGTACGTGATAAGGTTATCCCAAACAACATATATTTGGAGAACGGAAGTGGAAAAGGGTGGTTTTCGCATGTTCTTGAAGCAGGGCAAAGTAAAAGCAACCTTAAAACTGTTTCCCAATGTCTCCAAACAGAAAGGTTGCCGATATCGTCCCATCCAATGCAAGTTTCTATTAACAATGTTCACGAAACCGCTTTCTATCTGAATGATAAAAGCAAGGTGGACTTGTGGAGAGAGAGGACAGTTTGTGGTGCGGAGAATTCAGATAGAAGTCATGAAATTTCCAATAATAAGCATCAGAATATTTTTGTGACTTCTCATATGCCCAGTCCATATCATCACATTCTTCCTTCCTCGGATGTGGCCAGGTCATGGACTCACTCGGTTTCATCTTGGGAAAAGCCAGGCAATGGCGTAAGCCAAAAGCCAATATCAGTTCAAACTCACCCGTGTTTTCCTTCATCTGCTACCTTGAGCAAGAGTTCCCAGTCATCAGTTCAGAGCAATGGGATCTTTGGAGATAGGTGGTATCTTAACAGCAATTCTAGTTCTAACCATGGTTCTGGAAGTGAAGTACCATACCAAAATGGTTTTCACCATGGTTCATCTTCAGGGTCTAAGGAACTAGTTCGCTTCCCGTCTCTCAGCTGCGACTATCAGAGCAGCAGTAACGATCACAACGGAGGCCCTGAGCACTTGAGGAGTCAGGGTTCAGTGACACACTACAAGGGTTCAAGTTGCAAGGATTTGAAGTTTGCAAAGGAAGTAAACTTAAATGTGGTGCTTTCAAAGAGCTCATCCAATGAAGAAATACCCCAGCAATGTCTTAGGATCGTAGGGGGGGAACAAAAGCATGAGGACGATCTTGCAGCTTTGCCGTGGCTAAGAGCCAAGCCTGCTTTTAAGAATGAGTTTGCCAATGTAGGCAGAGTTTCAAAGACTGGTGTACAGGATTTAAATCAAACTTTTGCCGAGGACATAAAATCAGTTCCTTGCGAGAATGATGTTGAGGCCAGAAGGACTGAACTAGGTGACAGCCCGTGCAAGAGAAAACTTCTTGGGTTTCCTATTTTTGTTAAGTCCCATAACACTAAGAACGAGTCTTATTCTCTTACCTCCCCTTCTGTGGAAAATAACAAGAGAAATGGAGGACTGGATATCAACTTGCCTTGTGATCCTTCAGCTCCTGACTTGGCCACAAAGAATGTAGAAGAAATTGTAGTTGTAGACGAGAGAATAGATACGAAGGTTGCAAGTTTCAGACGTGTCATCGATTTGAACTCGTGTGTTAGTGATGATGAAGAATCTTTGAAACCTTCTGCTCCAGCCACCAGTGTGAAGATCACAGTAGAAATAGATTTGGAAGCCCCAATTGTACCCGGGACTGATGAAAGTGTCATTCTTGTGGAAGCGTCTCCTGAAAAGCAGAAAGCAATGCCTTTAGCATCGCCACAACACCCAGCTGAACCTCCTCAAGAGGAACTTGTGAGAGTAGCAGCGGAGGCAATAGTTGCCATCTCATCATCTGGTCCTGACAATCATACTGATGAATCCTCTTGCAGTCCACCGGAAGCTTCTTCAATCGACCCCCTTCTGTGGTTTGTGGAGATAGCTTCCACTTGTGGGAATGATCTTGAGAGCAAGTTTGACATCGTTGTGAGAGACAAAGATGGTGATGATGATGATGATGAATCTTTATCAGACGCGTTTGATTACTTTGAATTCGTGACTTTGAAACTGAGAGAGACCAAGGAAGAAGATTACATGCCTAAGCCTCTGGTTCCAGAAAACTTGAACCTGGAAGAAACAGGAAACACATTACTACCAAATCCTCCCAGAAGAGGCCAGTCAAGGAGAGGGAGGCAGCGGAGGGACTTCCAAAGGGATATCCTTCCCGGTCTGGCTTCCTTGTCAAGGCACGAGGTGACAGAAGATCTCCAGACATTTGGAGGATTAATGCGGGCAATGGGCCATTCATGGCAGACGGGACTAACCAGGAGAAACTCAACAAGAAACGGTTGTGGTAGGGGCAGGCGACGAGCAGTGGTGAATCCCTCTCCTCCTGTGGAAACTATCCCAGCTTGCAGTCCACTGGTACAGCAGCTTAATAATACAGAAATGGGACTGGAAGATAGAAGCCTAACCGGTTGGGGTAAGACAACCAGAAGGCCTCGTAGGCAAAGATGCGCGGCAGGTAATAATCTCCCATCATCCGTCCCTTTAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

1056

Amino Acids

116.82

Weight (kDa)

6.01

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 132 - 1042 0.0 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3122
Acc65I GGTACC 2 cut(s) 422, 869
AccB1I GGYRCC 2 cut(s) 422, 869
AccB7I CCANNNNNTGG 2 cut(s) 1311, 1349
AccI GTMKAC 1 cut(s) 2166
AccII CGCG 2 cut(s) 2645, 3128
AciI CCGC 7 cut(s) 881, 1131, 1193, 2438, 2806, 2899, 3128
AclWI GGATC 6 cut(s) 32, 33, 45, 1457, 1778, 2108
AcoI YGGCCR 2 cut(s) 1302, 2133
AcsI RAATTY 4 cut(s) 231, 1198, 1218, 2660
AcuI CTGAAG 4 cut(s) 360, 801, 1533, 2103
AdeI CACNNNGTG 1 cut(s) 2863
AfaI GTAC 8 cut(s) 227, 424, 871, 945, 1515, 1887, 2316, 3036
AfiI CCNNNNNNNGG 8 cut(s) 376, 1311, 1349, 2065, 2535, 2562, 2780, 2853
AflII CTTAAG 1 cut(s) 176
AflIII ACRYGT 4 cut(s) 727, 749, 2203, 2643
AgeI ACCGGT 1 cut(s) 3083
AhlI ACTAGT 1 cut(s) 1564
AjiI CACGTC 1 cut(s) 2204
AjnI CCWGG 6 cut(s) 139, 409, 1304, 1342, 2736, 2933
AjuI GAANNNNNNNTTGG 4 cut(s) 1090, 1122, 1515, 1547
AleI CACNNNNGTG 2 cut(s) 536, 2273
AloI GAACNNNNNNTCC 2 cut(s) 1633, 1665
Alw21I GWGCWC 3 cut(s) 764, 1635, 1736
Alw26I GTCTC 6 cut(s) 290, 1067, 1584, 2358, 2595, 2678
AlwI GGATC 6 cut(s) 32, 33, 45, 1457, 1778, 2108
AlwNI CAGNNNCTG 1 cut(s) 2126
Ama87I CYCGRG 3 cut(s) 5, 671, 2318
ApeKI GCWGC 7 cut(s) 898, 1587, 1602, 1808, 2435, 2803, 3040
ApoI RAATTY 4 cut(s) 231, 1198, 1218, 2660
AseI ATTAAT 1 cut(s) 2894
AsiGI ACCGGT 1 cut(s) 3083
Asp700I GAANNNNTTC 1 cut(s) 1566
Asp718I GGTACC 2 cut(s) 422, 869
AspLEI GCGC 1 cut(s) 3128
AspS9I GGNCC 7 cut(s) 57, 120, 398, 838, 1625, 2468, 2908
AsuC2I CCSGG 5 cut(s) 6, 7, 2319, 2320, 2835
AsuHPI GGTGA 9 cut(s) 209, 217, 526, 1376, 1526, 1982, 2624, 2875, 2998
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 3 cut(s) 5, 671, 2318
AvaII GGWCC 2 cut(s) 398, 2468
BaeI ACNNNNGTAYC 2 cut(s) 2172, 2205
BalI TGGCCA 2 cut(s) 1304, 2135
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 2 cut(s) 422, 869
BanII GRGCYC 1 cut(s) 1736
BauI CACGAG 2 cut(s) 2221, 2858
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 3 cut(s) 764, 1635, 1736
BbvI GCAGC 7 cut(s) 910, 1574, 1614, 1820, 2447, 2815, 3052
BccI CCATC 8 cut(s) 241, 409, 442, 511, 1101, 2462, 2603, 3154
BceAI ACGGC 2 cut(s) 428, 1801
BcgI CGANNNNNNTGC 4 cut(s) 106, 140, 662, 696
BciT130I CCWGG 6 cut(s) 141, 411, 1306, 1344, 2738, 2935
BcnI CCSGG 5 cut(s) 6, 7, 2319, 2320, 2835
BcoDI GTCTC 6 cut(s) 290, 1067, 1584, 2358, 2595, 2678
BcuI ACTAGT 1 cut(s) 1564
BfaI CTAG 5 cut(s) 438, 891, 1485, 1565, 1967
BfrI CTTAAG 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 3122
BglI GCCNNNNNGGC 1 cut(s) 3115
BglII AGATCT 1 cut(s) 2872
BisI GCNGC 9 cut(s) 882, 899, 1588, 1603, 1809, 2436, 2804, 3041, 3129
BlsI GCNGC 9 cut(s) 883, 900, 1589, 1604, 1810, 2437, 2805, 3042, 3130
Bme18I GGWCC 2 cut(s) 398, 2468
BmeT110I CYCGRG 3 cut(s) 5, 671, 2318
BmgBI CACGTC 1 cut(s) 2204
BmgT120I GGNCC 7 cut(s) 57, 120, 398, 838, 1625, 2468, 2908
BmiI GGNNCC 4 cut(s) 39, 424, 871, 2721
BmrI ACTGGG 2 cut(s) 1260, 1420
BmsI GCATC 6 cut(s) 121, 332, 334, 1243, 2393, 3113
BmuI ACTGGG 2 cut(s) 1260, 1420
BpiI GAAGAC 1 cut(s) 716
BpmI CTGGAG 2 cut(s) 2247, 2861
Bpu10I CCTNAGC 3 cut(s) 319, 1629, 2709
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 4 cut(s) 1432, 1657, 2397, 2594
BpuMI CCSGG 5 cut(s) 6, 7, 2319, 2320, 2835
Bsa29I ATCGAT 3 cut(s) 404, 740, 2211
BsaAI YACGTR 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 1896
BsaI GGTCTC 1 cut(s) 2678
BsaWI WCCGGW 2 cut(s) 2506, 3083
BsaXI ACNNNNNCTCC 4 cut(s) 415, 445, 1633, 1663
Bsc4I CCNNNNNNNGG 8 cut(s) 376, 1311, 1349, 2065, 2535, 2562, 2780, 2853
Bse118I RCCGGY 2 cut(s) 219, 3083
Bse1I ACTGG 8 cut(s) 1266, 1426, 1885, 2097, 2271, 2785, 3036, 3069
Bse3DI GCAATG 6 cut(s) 109, 1354, 1450, 1766, 2379, 2910
Bse8I GATNNNNATC 1 cut(s) 1896
BseBI CCWGG 6 cut(s) 141, 411, 1306, 1344, 2738, 2935
BseCI ATCGAT 3 cut(s) 404, 740, 2211
BseGI GGATG 6 cut(s) 160, 256, 1093, 1303, 1736, 3149
BseJI GATNNNNATC 1 cut(s) 1896
BseLI CCNNNNNNNGG 8 cut(s) 376, 1311, 1349, 2065, 2535, 2562, 2780, 2853
BseMI GCAATG 6 cut(s) 109, 1354, 1450, 1766, 2379, 2910
BseMII CTCAG 4 cut(s) 30, 1597, 1620, 2669
BseNI ACTGG 8 cut(s) 1266, 1426, 1885, 2097, 2271, 2785, 3036, 3069
BseRI GAGGAG 4 cut(s) 772, 1654, 2400, 2988
BseXI GCAGC 7 cut(s) 910, 1574, 1614, 1820, 2447, 2815, 3052
BseYI CCCAGC 3 cut(s) 1755, 2398, 3016
Bsh1236I CGCG 2 cut(s) 2645, 3128
BshNI GGYRCC 2 cut(s) 422, 869
BshTI ACCGGT 1 cut(s) 3083
BshVI ATCGAT 3 cut(s) 404, 740, 2211
BsiHKAI GWGCWC 3 cut(s) 764, 1635, 1736
BsiHKCI CYCGRG 3 cut(s) 5, 671, 2318
BsiSI CCGG 6 cut(s) 6, 220, 2319, 2507, 2835, 3084
BslFI GGGAC 9 cut(s) 254, 300, 1075, 2005, 2335, 2825, 2940, 3075, 3140
BslI CCNNNNNNNGG 8 cut(s) 376, 1311, 1349, 2065, 2535, 2562, 2780, 2853
BsmAI GTCTC 6 cut(s) 290, 1067, 1584, 2358, 2595, 2678
BsmBI CGTCTC 2 cut(s) 1584, 2358
BsmFI GGGAC 9 cut(s) 254, 300, 1075, 2005, 2335, 2825, 2940, 3075, 3140
Bso31I GGTCTC 1 cut(s) 2678
BsoBI CYCGRG 3 cut(s) 5, 671, 2318
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 3 cut(s) 764, 1635, 1736
Bsp1407I TGTACA 2 cut(s) 225, 1885
Bsp19I CCATGG 3 cut(s) 291, 1495, 1537
BspACI CCGC 7 cut(s) 881, 1131, 1193, 2438, 2806, 2899, 3128
BspCNI CTCAG 4 cut(s) 29, 1596, 1621, 2670
BspDI ATCGAT 3 cut(s) 404, 740, 2211
BspFNI CGCG 2 cut(s) 2645, 3128
BspHI TCATGA 1 cut(s) 1213
BspLI GGNNCC 4 cut(s) 39, 424, 871, 2721
BspMI ACCTGC 1 cut(s) 3122
BspPI GGATC 6 cut(s) 32, 33, 45, 1457, 1778, 2108
BspT104I TTCGAA 1 cut(s) 500
BspT107I GGYRCC 2 cut(s) 422, 869
BspTI CTTAAG 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 2678
BsrDI GCAATG 6 cut(s) 109, 1354, 1450, 1766, 2379, 2910
BsrFI RCCGGY 2 cut(s) 219, 3083
BsrGI TGTACA 2 cut(s) 225, 1885
BsrI ACTGG 8 cut(s) 1266, 1426, 1885, 2097, 2271, 2785, 3036, 3069
BssAI RCCGGY 2 cut(s) 219, 3083
BssSI CACGAG 2 cut(s) 2221, 2858
BssT1I CCWWGG 4 cut(s) 291, 1495, 1537, 2688
Bst2BI CACGAG 2 cut(s) 2221, 2858
Bst2UI CCWGG 6 cut(s) 141, 411, 1306, 1344, 2738, 2935
Bst4CI ACNGT 4 cut(s) 1051, 1183, 2287, 2957
Bst6I CTCTTC 1 cut(s) 2773
BstAFI CTTAAG 1 cut(s) 176
BstAPI GCANNNNNTGC 2 cut(s) 172, 2450
BstAUI TGTACA 2 cut(s) 225, 1885
BstBAI YACGTR 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 56, 1836, 2901, 2972, 3022
BstDSI CCRYGG 4 cut(s) 291, 1495, 1537, 1817
BstENI CCTNNNNNAGG 1 cut(s) 2851
BstF5I GGATG 6 cut(s) 160, 256, 1093, 1303, 1736, 3149
BstFNI CGCG 2 cut(s) 2645, 3128
BstHHI GCGC 1 cut(s) 3128
BstMAI GTCTC 6 cut(s) 290, 1067, 1584, 2358, 2595, 2678
BstMWI GCNNNNNNNGC 5 cut(s) 172, 1835, 2441, 2450, 3115
BstNI CCWGG 6 cut(s) 141, 411, 1306, 1344, 2738, 2935
BstNSI RCATGY 4 cut(s) 731, 753, 1013, 2707
BstUI CGCG 2 cut(s) 2645, 3128
BstV1I GCAGC 7 cut(s) 910, 1574, 1614, 1820, 2447, 2815, 3052
BstV2I GAAGAC 1 cut(s) 716
BstX2I RGATCY 4 cut(s) 25, 37, 1449, 2872
BstXI CCANNNNNNTGG 3 cut(s) 467, 1502, 2885
BstYI RGATCY 4 cut(s) 25, 37, 1449, 2872
Bsu15I ATCGAT 3 cut(s) 404, 740, 2211
BsuTUI ATCGAT 3 cut(s) 404, 740, 2211
BtgI CCRYGG 4 cut(s) 291, 1495, 1537, 1817
BtgZI GCGATG 1 cut(s) 2370
BtrI CACGTC 1 cut(s) 2204
BtsCI GGATG 6 cut(s) 160, 256, 1093, 1303, 1736, 3149
BtsI GCAGTG 1 cut(s) 2988
BtsIMutI CAGTG 6 cut(s) 24, 609, 1659, 2278, 2988, 3029
BveI ACCTGC 1 cut(s) 3122
Cac8I GCNNGC 5 cut(s) 56, 1836, 2901, 2972, 3022
CaiI CAGNNNCTG 1 cut(s) 2126
CciI TCATGA 1 cut(s) 1213
CfoI GCGC 1 cut(s) 3128
Cfr10I RCCGGY 2 cut(s) 219, 3083
Cfr13I GGNCC 7 cut(s) 57, 120, 398, 838, 1625, 2468, 2908
Cfr9I CCCGGG 2 cut(s) 5, 2318
ClaI ATCGAT 3 cut(s) 404, 740, 2211
CseI GACGC 2 cut(s) 2340, 2651
Csp6I GTAC 8 cut(s) 226, 423, 870, 944, 1514, 1886, 2315, 3035
CspAI ACCGGT 1 cut(s) 3083
CviQI GTAC 8 cut(s) 226, 423, 870, 944, 1514, 1886, 2315, 3035
DraI TTTAAA 1 cut(s) 1896
DraIII CACNNNGTG 1 cut(s) 2863
EaeI YGGCCR 2 cut(s) 1302, 2133
Eam1104I CTCTTC 1 cut(s) 2773
EarI CTCTTC 1 cut(s) 2773
Ecl136II GAGCTC 1 cut(s) 1734
Eco130I CCWWGG 4 cut(s) 291, 1495, 1537, 2688
Eco147I AGGCCT 1 cut(s) 3109
Eco24I GRGCYC 1 cut(s) 1736
Eco31I GGTCTC 1 cut(s) 2678
Eco32I GATATC 3 cut(s) 1086, 2098, 2827
Eco47I GGWCC 2 cut(s) 398, 2468
Eco53kI GAGCTC 1 cut(s) 1734
Eco57I CTGAAG 4 cut(s) 360, 801, 1533, 2103
Eco88I CYCGRG 3 cut(s) 5, 671, 2318
EcoICRI GAGCTC 1 cut(s) 1734
EcoNI CCTNNNNNAGG 1 cut(s) 2851
EcoO109I RGGNCCY 1 cut(s) 1625
EcoRI GAATTC 3 cut(s) 231, 1198, 2660
EcoRII CCWGG 6 cut(s) 139, 409, 1304, 1342, 2736, 2933
EcoRV GATATC 3 cut(s) 1086, 2098, 2827
EcoT14I CCWWGG 4 cut(s) 291, 1495, 1537, 2688
EcoT38I GRGCYC 1 cut(s) 1736
ErhI CCWWGG 4 cut(s) 291, 1495, 1537, 2688
Esp3I CGTCTC 2 cut(s) 1584, 2358
FalI AAGNNNNNCTT 4 cut(s) 1148, 1180, 2406, 2438
FaqI GGGAC 9 cut(s) 254, 300, 1075, 2005, 2335, 2825, 2940, 3075, 3140
FauI CCCGC 1 cut(s) 2892
FauNDI CATATG 1 cut(s) 1260
FblI GTMKAC 1 cut(s) 2166
Fnu4HI GCNGC 9 cut(s) 882, 899, 1588, 1603, 1809, 2436, 2804, 3041, 3129
FokI GGATG 6 cut(s) 167, 263, 1080, 1310, 1723, 3136
FriOI GRGCYC 1 cut(s) 1736
Fsp4HI GCNGC 9 cut(s) 882, 899, 1588, 1603, 1809, 2436, 2804, 3041, 3129
FspBI CTAG 5 cut(s) 438, 891, 1485, 1565, 1967
GlaI GCGC 1 cut(s) 3127
GluI GCNGC 9 cut(s) 882, 899, 1588, 1603, 1809, 2436, 2804, 3041, 3129
GsaI CCCAGC 3 cut(s) 1759, 2402, 3020
GsuI CTGGAG 2 cut(s) 2247, 2861
HapII CCGG 6 cut(s) 6, 220, 2319, 2507, 2835, 3084
HgaI GACGC 2 cut(s) 2340, 2651
HhaI GCGC 1 cut(s) 3128
Hin6I GCGC 1 cut(s) 3126
HinP1I GCGC 1 cut(s) 3126
HindIII AAGCTT 2 cut(s) 621, 2511
HpaII CCGG 6 cut(s) 6, 220, 2319, 2507, 2835, 3084
HphI GGTGA 9 cut(s) 209, 217, 526, 1376, 1526, 1982, 2624, 2875, 2998
Hpy166II GTNNAC 9 cut(s) 382, 609, 1123, 1162, 1708, 1886, 2167, 2504, 3029
Hpy8I GTNNAC 9 cut(s) 382, 609, 1123, 1162, 1708, 1886, 2167, 2504, 3029
Hpy99I CGWCG 1 cut(s) 2979
HpyCH4III ACNGT 4 cut(s) 1051, 1183, 2287, 2957
HpyCH4IV ACGT 2 cut(s) 946, 2203
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 1835, 2441, 2450, 3115
HpySE526I ACGT 2 cut(s) 946, 2203
HspAI GCGC 1 cut(s) 3126
KpnI GGTACC 2 cut(s) 426, 873
LmnI GCTCC 4 cut(s) 601, 759, 2128, 2266
Lsp1109I GCAGC 7 cut(s) 910, 1574, 1614, 1820, 2447, 2815, 3052
LweI GCATC 6 cut(s) 121, 332, 334, 1243, 2393, 3113
MaeI CTAG 5 cut(s) 438, 891, 1485, 1565, 1967
MaeII ACGT 2 cut(s) 946, 2203
MaeIII GTNAC 6 cut(s) 1249, 1607, 1654, 1970, 2665, 2863
MfeI CAATTG 2 cut(s) 46, 2310
MflI RGATCY 4 cut(s) 25, 37, 1449, 2872
MhlI GDGCHC 3 cut(s) 764, 1635, 1736
MlsI TGGCCA 2 cut(s) 1304, 2135
MluI ACGCGT 1 cut(s) 2643
MluNI TGGCCA 2 cut(s) 1304, 2135
MlyI GAGTC 5 cut(s) 530, 839, 1309, 1651, 2048
Mox20I TGGCCA 2 cut(s) 1304, 2135
MroXI GAANNNNTTC 1 cut(s) 1566
MscI TGGCCA 2 cut(s) 1304, 2135
MslI CAYNNNNRTG 6 cut(s) 536, 945, 2273, 2342, 2484, 2594
Msp20I TGGCCA 2 cut(s) 1304, 2135
MspA1I CMGCKG 5 cut(s) 572, 1587, 2402, 2438, 2806
MspCI CTTAAG 1 cut(s) 176
MspI CCGG 6 cut(s) 6, 220, 2319, 2507, 2835, 3084
MunI CAATTG 2 cut(s) 46, 2310
MvaI CCWGG 6 cut(s) 141, 411, 1306, 1344, 2738, 2935
MvnI CGCG 2 cut(s) 2645, 3128
MwoI GCNNNNNNNGC 5 cut(s) 172, 1835, 2441, 2450, 3115
NciI CCSGG 5 cut(s) 6, 7, 2319, 2320, 2835
NcoI CCATGG 3 cut(s) 291, 1495, 1537
NdeI CATATG 1 cut(s) 1260
NlaIV GGNNCC 4 cut(s) 39, 424, 871, 2721
NmeAIII GCCGAG 2 cut(s) 141, 1936
NmuCI GTSAC 5 cut(s) 1249, 1654, 1970, 2665, 2863
NspI RCATGY 4 cut(s) 731, 753, 1013, 2707
NspV TTCGAA 1 cut(s) 500
OliI CACNNNNGTG 2 cut(s) 536, 2273
PaeR7I CTCGAG 1 cut(s) 671
PagI TCATGA 1 cut(s) 1213
PceI AGGCCT 1 cut(s) 3109
PciI ACATGT 2 cut(s) 727, 749
PdmI GAANNNNTTC 1 cut(s) 1566
PfeI GAWTC 9 cut(s) 199, 362, 406, 587, 862, 2243, 2489, 2630, 2989
PflMI CCANNNNNTGG 2 cut(s) 1311, 1349
PinAI ACCGGT 1 cut(s) 3083
PkrI GCNGC 9 cut(s) 883, 900, 1589, 1604, 1810, 2437, 2805, 3042, 3130
PleI GAGTC 5 cut(s) 529, 839, 1309, 1650, 2047
PpsI GAGTC 5 cut(s) 529, 839, 1309, 1650, 2047
Ppu21I YACGTR 1 cut(s) 947
PscI ACATGT 2 cut(s) 727, 749
PshBI ATTAAT 1 cut(s) 2894
Psp124BI GAGCTC 1 cut(s) 1736
Psp6I CCWGG 6 cut(s) 139, 409, 1304, 1342, 2736, 2933
PspFI CCCAGC 3 cut(s) 1755, 2398, 3016
PspGI CCWGG 6 cut(s) 139, 409, 1304, 1342, 2736, 2933
PspN4I GGNNCC 4 cut(s) 39, 424, 871, 2721
PspPI GGNCC 7 cut(s) 57, 120, 398, 838, 1625, 2468, 2908
PspXI VCTCGAGB 1 cut(s) 671
PstNI CAGNNNCTG 1 cut(s) 2126
PsuI RGATCY 4 cut(s) 25, 37, 1449, 2872
PvuII CAGCTG 3 cut(s) 572, 1587, 2402
RsaI GTAC 8 cut(s) 227, 424, 871, 945, 1515, 1887, 2316, 3036
RsaNI GTAC 8 cut(s) 226, 423, 870, 944, 1514, 1886, 2315, 3035
RseI CAYNNNNRTG 6 cut(s) 536, 945, 2273, 2342, 2484, 2594
SacI GAGCTC 1 cut(s) 1736
SatI GCNGC 9 cut(s) 882, 899, 1588, 1603, 1809, 2436, 2804, 3041, 3129
Sau96I GGNCC 7 cut(s) 57, 120, 398, 838, 1625, 2468, 2908
SchI GAGTC 5 cut(s) 530, 839, 1309, 1651, 2048
SduI GDGCHC 3 cut(s) 764, 1635, 1736
SfaNI GCATC 6 cut(s) 121, 332, 334, 1243, 2393, 3113
Sfr274I CTCGAG 1 cut(s) 671
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 2 cut(s) 398, 2468
SlaI CTCGAG 1 cut(s) 671
SmaI CCCGGG 2 cut(s) 7, 2320
SmiI ATTTAAAT 1 cut(s) 1896
SmiMI CAYNNNNRTG 6 cut(s) 536, 945, 2273, 2342, 2484, 2594
SmlI CTYRAG 6 cut(s) 176, 671, 1411, 1636, 2412, 2573
SmoI CTYRAG 6 cut(s) 176, 671, 1411, 1636, 2412, 2573
SpeI ACTAGT 1 cut(s) 1564
SseBI AGGCCT 1 cut(s) 3109
SsiI CCGC 7 cut(s) 881, 1131, 1193, 2438, 2806, 2899, 3128
SspI AATATT 1 cut(s) 1243
SspMI CTAG 5 cut(s) 438, 891, 1485, 1565, 1967
SstI GAGCTC 1 cut(s) 1736
StuI AGGCCT 1 cut(s) 3109
StyI CCWWGG 4 cut(s) 291, 1495, 1537, 2688
SwaI ATTTAAAT 1 cut(s) 1896
TaaI ACNGT 4 cut(s) 1051, 1183, 2287, 2957
TaiI ACGT 2 cut(s) 949, 2206
TaqI TCGA 7 cut(s) 404, 500, 672, 740, 755, 2211, 2523
TatI WGTACW 2 cut(s) 225, 1885
TauI GCSGC 2 cut(s) 884, 3131
TfiI GAWTC 9 cut(s) 199, 362, 406, 587, 862, 2243, 2489, 2630, 2989
TscAI CASTG 6 cut(s) 24, 616, 1659, 2278, 2988, 3036
TseFI GTSAC 5 cut(s) 1249, 1654, 1970, 2665, 2863
TseI GCWGC 7 cut(s) 898, 1587, 1602, 1808, 2435, 2803, 3040
Tsp45I GTSAC 5 cut(s) 1249, 1654, 1970, 2665, 2863
TspGWI ACGGA 4 cut(s) 84, 999, 1635, 3142
TspMI CCCGGG 2 cut(s) 5, 2318
TspRI CASTG 6 cut(s) 24, 616, 1659, 2278, 2988, 3036
Van91I CCANNNNNTGG 2 cut(s) 1311, 1349
Vha464I CTTAAG 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 398, 2468
VspI ATTAAT 1 cut(s) 2894
XagI CCTNNNNNAGG 1 cut(s) 2851
XapI RAATTY 4 cut(s) 231, 1198, 1218, 2660
XbaI TCTAGA 1 cut(s) 890
XceI RCATGY 4 cut(s) 731, 753, 1013, 2707
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 671
XmaI CCCGGG 2 cut(s) 5, 2318
XmiI GTMKAC 1 cut(s) 2166
XmnI GAANNNNTTC 1 cut(s) 1566
XspI CTAG 5 cut(s) 438, 891, 1485, 1565, 1967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.