RchiOBHm_Chr4g0438891

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007903
Physical Location & Seq
Reverse (-)
61421490 .. 61427793
6304 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40698

Sequence Viewer

Length: 3207 bp
ATGGGAACAAAAATGCAGTGCAAAAGCTATTTGCCGGGGTATTACTCAGTTAGGGATCTTAATGAGGATCCGAACAATTGTAGCTGGCCCTTATACTATGGAGATAAAACCTTGCCAAACACTCAATATTACAATGGTTTCTTGCCAAGGGCCGCTGTCGATACTTATCAAGGGTATGGTAAAGACATAGTAAAGGAGACAATGCTTCAGCATGAGGCCATATTTAAGAATCAGGTGTATGAACTTCATCGCCTGTACAGAATACAAAGGGACTTGATGGATGAAATCAAAAGAAAAGAACTGCATAGAAACCATATGCCCATGGAGGCATCATTATCCTCAAGTCCCTTAGCATCTCAAATTACTTCTGAACATACTCGGAAATGGCCTGATTCCAGCTTCCCTTTGGTGAACTCGGTTTATGCGGGACCGTCAAGTTCAGGTGTTGAAGGCATTCATTCTCAATCTAGTGCTATTAAAGGGAATGGGCGAAAAAATGGTCTATATCCGTGTCAAAATGGTATTAGTTCAAAAGATGTTGAGGTGTTGGATTCAAGACCTACAAAAGTGAGGAAAAAAATGTTTGACCTTCAACTTCCAGCTGATGTGTACATAGATTCTGAAGAAGGGGAAGAGTGCAGTGACGAGAAAGTTTCTTGCATGCCTAGTTGTCTGTCAACTAAAAATTGTAAAATCGCACCTGAGGGTGGTGGGAAGGTCTTTTTGGGTGATGGCGGAAAGTCTGACTTTGCAGGGGATGCGTTGAGATCAGAGAAATGTCCAAGGGGTGCAAATGGTTTTGCTGACTTGAATGAGCCCATTCAACCTGAAGAAGCAAGTGCTTCAGGATATAATGATCCTCCGGGCCATGACTCTTTTCGGGGGAAGATTCAAGGCCCTGATCTGCCTGCTAAGTCAAGGTCACAGCTTCTGGGTGATGGAGCAAAAAATGGGTGGTTTTCCCATGTTCTTGAATCAGGGCCAAATAAGAGCAACCTCAAAACTGTGTCCCAGTGTCCGCAAACGGAAAGGTTGCCTATATCATCCCAGCCGGTCCAAGCTTCTGTGAACAATGTTCATGAACAAAATTTCTATTTGACGGATAAAAGCAAGGTGAATTTGTGGAGAGAGAGGACAATTTCTGGTGTTGAGAGTTCCGAAAGAAGTAACGAGTTTTCCAGTAACAAGCACCCGAGTACCTTTGTGGCATCTAATGTGCCCAGTCCTTATCCGATTCTTCCTTCTTCTGATTTGGCCAAGTCCTGGACTCACTCGGTTTCTTCTTGGGAAAAGGCAGGCAGTAGCTTAAGCCAGAAGTCAATGTCAGTTCAAGCTCACCCATGTTTTAATTCATCTGCTACCTTGAGTAAGAGTTCCCAGTCATCGGTTCAGAGTAATGGGATTTTCGGAGACAGGTGGAATCTCAACAACAATTCTAGATCTAACCAAGGTTCAGGAAGTGAATTGCTGTACCAAAATGGGTTTCATCATGGTTCCTCTTCTGGGTCTAAGGAGCAACTGGTCCGCTTTCCTTCTCTCAACTGTGACTATCAGAGCAGCAGTATCAATCATAATGGAGGTTCTGAACACTTGATGAGTCATGGTTCAGCAACGTACTACAAGGGTTCAAATTTCTTGGATGTGAAGTCTGCAAAGGAAGTAAACTTGAACGTGATGCTTTCAAACAGTTCATCTAATGAAGAATTACCCCAGCGAGGTCTTAAGATCATGGGTGGAGAACAAAAGCATGAGGACCCTCTTGCAGCCTTGCCATGGCTAAGAGCTAAGCCTGCTGGTAAGAATGAGTTTGCCAATGGTGGCGGAGTTTCTAAGACCAGTGAGCCTAGTATCTTTCAGTCTTCTGTGAATAATTTATCCAATAAAATTGAAGCTGGAAAGGGTTTCAATCAAATTTTTACTCAAAGTATAAAATCATTTTCATGCGGGAATGATGTTGAGGCGAGGAGGACTGAACTAGCTGATAGCCCGAGTAATAAAAAACTTCTTGGATTTCCTATTTTTGAGAAGTCTCAGCTTTTGAAGAATGAGTCCTTTTCTCTCACCTCCCCTTCTGTGTCTATTCCTCACCCATCGGAGAGTGAAGTGGAAAATAACAGAAGAAATAGATTGCTGGACATCAACTTGCCTTGTGATACTGCAGCTCCTGACTTGGCCAGAAAGAACGTAGCAGAAATTGTAGTGGTAGAAGACAGAAGGGATAAACAGTTTGGTAATTTCAGACATCACATTGACCTGAACTTCTGTATCAGCGAAGATGAAGCATCTTTGAAACCTTCTGCTCCAAGAGGCAGTATGAAGATTGCAGTGGAGATAGATTTGGAAGCCCCCATTTCTCCGGAGACTGATGATGAGGATGATGTCATTCATGGGGAAGCATGTGCACAAAAGCAGAACAAGATGTCTTTAGCATTACCACAGAAAAAAACTGAACCTTCTCGAGATGAGCTTGCAAGAGTAGCAGCAGAGGCAATTGTTGCCATTTCATCATGCGGTCCTCCTGATCCTTTGGATGAATCCTCTTGCAAACTAGCAGAAGCATCTTCAGTTGACCCTCTTATGTGGTTTGTGGACGTAGTTTCTACATGTGGGAATGATCTTGAGAACAAGTTTGACATTGTTCTCAGGAGTGACGATAGTGAAGGTATTGAAGAATCTTTAGTAGAAGAGTTTGATTACTTTGAATTCCTCACTTTGAAACTGAAGGAGACCAAGGAAGAAGATTACATGCCCAAGCCTTTGGTTCCGGAAAATATAAAGTTTGAAGAGTCAGGAACCAATTTACTATCAAATACGCCCCGAAGGGGCCAGTCAAGGAGAGGGAGGCAGCGGCGGGACTTCCAAAGGGATATCCTTCCCGGCCTCGTTTCTTTGTCAAGGCATGAGGTAACAGAAGATATCCAGACATTTGGAGGGTTGATGCGGGCAACAGGTCATCCTTCATGGCAATCGGGGTTGGCAAGAAGGAACTCAACAAGAAATGGTTGTGCTAGGGGCAGGCGACGAGCAGTGGCGAGTCCTTCACCCCCTATGATCACCATCCCGGTTTGCACTCCACCAATACAGCAGTTTAGTAATACCGAAATGGCACTGGAAGATAGAAGCCTAACAGGTTGGGGTAAGACTACTAGAAGGCCCCGTAGGCAAAGATGCGCGGCAGGTAATCCTCCCTCTCTTCCTTTAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

1068

Amino Acids

117.78

Weight (kDa)

6.3

Isoelectric Point (pI)

58.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 141 - 1057 0.0 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3167
AccB7I CCANNNNNTGG 1 cut(s) 1263
AccII CGCG 1 cut(s) 3173
AccIII TCCGGA 2 cut(s) 2356, 2764
AclWI GGATC 5 cut(s) 62, 63, 75, 851, 2516
AcoI YGGCCR 2 cut(s) 1254, 2172
AcsI RAATTY 5 cut(s) 1087, 1117, 1630, 1911, 2702
AcuI CTGAAG 6 cut(s) 191, 642, 828, 849, 2547, 2741
AfaI GTAC 5 cut(s) 257, 611, 1198, 1472, 1616
AfiI CCNNNNNNNGG 7 cut(s) 707, 1263, 1384, 1503, 1715, 1773, 2822
AflII CTTAAG 2 cut(s) 1306, 1721
AflIII ACRYGT 1 cut(s) 2603
AjnI CCWGG 1 cut(s) 1262
AjuI GAANNNNNNNTTGG 4 cut(s) 707, 739, 1467, 1499
Alw21I GWGCWC 1 cut(s) 2404
Alw26I GTCTC 5 cut(s) 191, 1404, 2034, 2354, 2720
Alw44I GTGCAC 1 cut(s) 2400
AlwI GGATC 5 cut(s) 62, 63, 75, 851, 2516
AlwNI CAGNNNCTG 2 cut(s) 931, 2165
Ama87I CYCGRG 3 cut(s) 1192, 1987, 2457
Aor13HI TCCGGA 2 cut(s) 2356, 2764
ApaLI GTGCAC 1 cut(s) 2400
ApeKI GCWGC 5 cut(s) 1557, 1763, 2159, 2480, 2845
ApoI RAATTY 5 cut(s) 1087, 1117, 1630, 1911, 2702
ArsI GACNNNNNNTTYG 2 cut(s) 484, 516
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 3173
AsuC2I CCSGG 4 cut(s) 36, 864, 2877, 3062
AsuHPI GGTGA 9 cut(s) 421, 740, 947, 1126, 1328, 2053, 2078, 3033, 3046
AvaI CYCGRG 3 cut(s) 1192, 1987, 2457
AvaII GGWCC 5 cut(s) 428, 1054, 1522, 1753, 2513
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 2 cut(s) 1221, 2404
BaeI ACNNNNGTAYC 4 cut(s) 1180, 1213, 2248, 2281
BalI TGGCCA 2 cut(s) 1256, 2174
BamHI GGATCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 819
BbsI GAAGAC 2 cut(s) 1851, 2214
Bbv12I GWGCWC 1 cut(s) 2404
BbvI GCAGC 5 cut(s) 1569, 1775, 2171, 2492, 2857
BccI CCATC 5 cut(s) 271, 725, 932, 2098, 3065
BciT130I CCWGG 1 cut(s) 1264
BclI TGATCA 1 cut(s) 3051
BcnI CCSGG 4 cut(s) 36, 864, 2877, 3062
BcoDI GTCTC 5 cut(s) 191, 1404, 2034, 2354, 2720
BfaI CTAG 8 cut(s) 468, 666, 1437, 1845, 1976, 2549, 3009, 3147
BfmI CTRYAG 1 cut(s) 2157
BfrI CTTAAG 2 cut(s) 1306, 1721
BfuAI ACCTGC 1 cut(s) 3167
BglI GCCNNNNNGGC 1 cut(s) 3160
BglII AGATCT 1 cut(s) 1439
BisI GCNGC 8 cut(s) 153, 1558, 1764, 2160, 2481, 2846, 2849, 3174
BlpI GCTNAGC 1 cut(s) 1785
BlsI GCNGC 8 cut(s) 154, 1559, 1765, 2161, 2482, 2847, 2850, 3175
Bme1390I CCNGG 5 cut(s) 36, 864, 1264, 2877, 3062
Bme18I GGWCC 5 cut(s) 428, 1054, 1522, 1753, 2513
BmeT110I CYCGRG 3 cut(s) 1192, 1987, 2457
BmiI GGNNCC 8 cut(s) 69, 429, 1495, 1755, 2763, 2794, 2825, 3155
BmrFI CCNGG 5 cut(s) 36, 864, 1264, 2877, 3062
BmrI ACTGGG 3 cut(s) 1006, 1215, 1372
BmsI GCATC 9 cut(s) 338, 362, 748, 1217, 1665, 2291, 2567, 2928, 3158
BmuI ACTGGG 3 cut(s) 1006, 1215, 1372
BpiI GAAGAC 2 cut(s) 1851, 2214
Bpu10I CCTNAGC 1 cut(s) 349
Bpu1102I GCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 3 cut(s) 325, 1384, 2639
BpuMI CCSGG 4 cut(s) 36, 864, 2877, 3062
BsaBI GATNNNNATC 2 cut(s) 165, 3056
BsaI GGTCTC 1 cut(s) 2720
BsaJI CCNNGG 7 cut(s) 35, 146, 321, 782, 1447, 1772, 2730
BsaWI WCCGGW 2 cut(s) 2356, 2764
BsaXI ACNNNNNCTCC 2 cut(s) 2146, 2176
Bsc4I CCNNNNNNNGG 7 cut(s) 707, 1263, 1384, 1503, 1715, 1773, 2822
Bse118I RCCGGY 1 cut(s) 1051
Bse1I ACTGG 8 cut(s) 1012, 1179, 1221, 1378, 1524, 1836, 2827, 3114
Bse21I CCTNAGG 1 cut(s) 702
Bse8I GATNNNNATC 2 cut(s) 165, 3056
BseAI TCCGGA 2 cut(s) 2356, 2764
BseBI CCWGG 1 cut(s) 1264
BseDI CCNNGG 7 cut(s) 35, 146, 321, 782, 1447, 1772, 2730
BseGI GGATG 8 cut(s) 286, 763, 1043, 1645, 2380, 2536, 2953, 3057
BseJI GATNNNNATC 2 cut(s) 165, 3056
BseLI CCNNNNNNNGG 7 cut(s) 707, 1263, 1384, 1503, 1715, 1773, 2822
BseMII CTCAG 4 cut(s) 60, 693, 2045, 2656
BseNI ACTGG 8 cut(s) 1012, 1179, 1221, 1378, 1524, 1836, 2827, 3114
BseRI GAGGAG 1 cut(s) 1978
BseSI GKGCMC 2 cut(s) 1221, 2404
BseXI GCAGC 5 cut(s) 1569, 1775, 2171, 2492, 2857
BseYI CCCAGC 2 cut(s) 1047, 1710
BsgI GTGCAG 1 cut(s) 658
Bsh1236I CGCG 1 cut(s) 3173
BsiHKAI GWGCWC 1 cut(s) 2404
BsiHKCI CYCGRG 3 cut(s) 1192, 1987, 2457
BsiSI CCGG 7 cut(s) 35, 863, 1052, 2357, 2765, 2877, 3062
BslFI GGGAC 5 cut(s) 284, 330, 441, 994, 2867
BslI CCNNNNNNNGG 7 cut(s) 707, 1263, 1384, 1503, 1715, 1773, 2822
BsmAI GTCTC 5 cut(s) 191, 1404, 2034, 2354, 2720
BsmFI GGGAC 5 cut(s) 284, 330, 441, 994, 2867
BsmI GAATGC 1 cut(s) 453
Bso31I GGTCTC 1 cut(s) 2720
BsoBI CYCGRG 3 cut(s) 1192, 1987, 2457
Bsp1286I GDGCHC 3 cut(s) 819, 1221, 2404
Bsp13I TCCGGA 2 cut(s) 2356, 2764
Bsp1407I TGTACA 2 cut(s) 255, 609
Bsp1720I GCTNAGC 1 cut(s) 1785
Bsp19I CCATGG 2 cut(s) 321, 1772
BspCNI CTCAG 4 cut(s) 59, 694, 2044, 2655
BspEI TCCGGA 2 cut(s) 2356, 2764
BspFNI CGCG 1 cut(s) 3173
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 8 cut(s) 69, 429, 1495, 1755, 2763, 2794, 2825, 3155
BspMAI CTGCAG 1 cut(s) 2161
BspMI ACCTGC 1 cut(s) 3167
BspPI GGATC 5 cut(s) 62, 63, 75, 851, 2516
BspTI CTTAAG 2 cut(s) 1306, 1721
BspTNI GGTCTC 1 cut(s) 2720
BsrFI RCCGGY 1 cut(s) 1051
BsrGI TGTACA 2 cut(s) 255, 609
BsrI ACTGG 8 cut(s) 1012, 1179, 1221, 1378, 1524, 1836, 2827, 3114
BssAI RCCGGY 1 cut(s) 1051
BssECI CCNNGG 7 cut(s) 35, 146, 321, 782, 1447, 1772, 2730
BssT1I CCWWGG 6 cut(s) 146, 321, 782, 1447, 1772, 2730
Bst2UI CCWGG 1 cut(s) 1264
Bst4CI ACNGT 5 cut(s) 432, 1006, 1544, 1688, 2226
Bst6I CTCTTC 5 cut(s) 627, 1504, 2679, 2778, 3198
BstAFI CTTAAG 2 cut(s) 1306, 1721
BstAPI GCANNNNNTGC 2 cut(s) 758, 2495
BstAUI TGTACA 2 cut(s) 255, 609
BstC8I GCNNGC 8 cut(s) 86, 662, 909, 1297, 1791, 2469, 2943, 3017
BstDSI CCRYGG 2 cut(s) 321, 1772
BstF5I GGATG 8 cut(s) 286, 763, 1043, 1645, 2380, 2536, 2953, 3057
BstFNI CGCG 1 cut(s) 3173
BstHHI GCGC 1 cut(s) 3173
BstMAI GTCTC 5 cut(s) 191, 1404, 2034, 2354, 2720
BstMWI GCNNNNNNNGC 6 cut(s) 758, 1790, 2477, 2486, 2495, 3160
BstNI CCWGG 1 cut(s) 1264
BstNSI RCATGY 4 cut(s) 664, 2400, 2607, 2749
BstSCI CCNGG 5 cut(s) 34, 862, 1262, 2875, 3060
BstSFI CTRYAG 1 cut(s) 2157
BstSLI GKGCMC 2 cut(s) 1221, 2404
BstUI CGCG 1 cut(s) 3173
BstV1I GCAGC 5 cut(s) 1569, 1775, 2171, 2492, 2857
BstV2I GAAGAC 2 cut(s) 1851, 2214
BstX2I RGATCY 3 cut(s) 55, 67, 1439
BstXI CCANNNNNNTGG 2 cut(s) 2758, 2927
BstYI RGATCY 3 cut(s) 55, 67, 1439
Bsu36I CCTNAGG 1 cut(s) 702
BtgI CCRYGG 2 cut(s) 321, 1772
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 8 cut(s) 286, 763, 1043, 1645, 2380, 2536, 2953, 3057
BtsI GCAGTG 4 cut(s) 23, 646, 2331, 3033
BtsIMutI CAGTG 7 cut(s) 23, 646, 1019, 1843, 2331, 3033, 3107
BveI ACCTGC 1 cut(s) 3167
Cac8I GCNNGC 8 cut(s) 86, 662, 909, 1297, 1791, 2469, 2943, 3017
CaiI CAGNNNCTG 2 cut(s) 931, 2165
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 1 cut(s) 3173
Cfr10I RCCGGY 1 cut(s) 1051
Csp6I GTAC 5 cut(s) 256, 610, 1197, 1471, 1615
CviQI GTAC 5 cut(s) 256, 610, 1197, 1471, 1615
EaeI YGGCCR 2 cut(s) 1254, 2172
Eam1104I CTCTTC 5 cut(s) 627, 1504, 2679, 2778, 3198
EarI CTCTTC 5 cut(s) 627, 1504, 2679, 2778, 3198
EciI GGCGGA 2 cut(s) 750, 1836
Eco130I CCWWGG 6 cut(s) 146, 321, 782, 1447, 1772, 2730
Eco24I GRGCYC 1 cut(s) 819
Eco31I GGTCTC 1 cut(s) 2720
Eco32I GATATC 2 cut(s) 2869, 2917
Eco47I GGWCC 5 cut(s) 428, 1054, 1522, 1753, 2513
Eco57I CTGAAG 6 cut(s) 191, 642, 828, 849, 2547, 2741
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 3 cut(s) 1192, 1987, 2457
EcoO109I RGGNCCY 3 cut(s) 896, 1753, 3153
EcoRI GAATTC 1 cut(s) 2702
EcoRII CCWGG 1 cut(s) 1262
EcoRV GATATC 2 cut(s) 2869, 2917
EcoT14I CCWWGG 6 cut(s) 146, 321, 782, 1447, 1772, 2730
EcoT38I GRGCYC 1 cut(s) 819
ErhI CCWWGG 6 cut(s) 146, 321, 782, 1447, 1772, 2730
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 5 cut(s) 284, 330, 441, 994, 2867
FauI CCCGC 4 cut(s) 418, 1937, 2844, 2934
FauNDI CATATG 1 cut(s) 315
FbaI TGATCA 1 cut(s) 3051
Fnu4HI GCNGC 8 cut(s) 153, 1558, 1764, 2160, 2481, 2846, 2849, 3174
FokI GGATG 8 cut(s) 293, 770, 1030, 1652, 2387, 2543, 2940, 3044
FriOI GRGCYC 1 cut(s) 819
Fsp4HI GCNGC 8 cut(s) 153, 1558, 1764, 2160, 2481, 2846, 2849, 3174
FspBI CTAG 8 cut(s) 468, 666, 1437, 1845, 1976, 2549, 3009, 3147
GlaI GCGC 1 cut(s) 3172
GluI GCNGC 8 cut(s) 153, 1558, 1764, 2160, 2481, 2846, 2849, 3174
GsaI CCCAGC 2 cut(s) 1051, 1714
HapII CCGG 7 cut(s) 35, 863, 1052, 2357, 2765, 2877, 3062
HhaI GCGC 1 cut(s) 3173
Hin6I GCGC 1 cut(s) 3171
HinP1I GCGC 1 cut(s) 3171
HincII GTYRAC 2 cut(s) 678, 2569
HindII GTYRAC 2 cut(s) 678, 2569
HindIII AAGCTT 1 cut(s) 1059
HpaII CCGG 7 cut(s) 35, 863, 1052, 2357, 2765, 2877, 3062
HphI GGTGA 9 cut(s) 421, 740, 947, 1126, 1328, 2053, 2078, 3033, 3046
Hpy166II GTNNAC 8 cut(s) 412, 610, 678, 1069, 1663, 2402, 2569, 2590
Hpy8I GTNNAC 8 cut(s) 412, 610, 678, 1069, 1663, 2402, 2569, 2590
Hpy99I CGWCG 1 cut(s) 3024
HpyCH4III ACNGT 5 cut(s) 432, 1006, 1544, 1688, 2226
HpyCH4IV ACGT 4 cut(s) 1613, 1671, 2184, 2592
HpyF10VI GCNNNNNNNGC 6 cut(s) 758, 1790, 2477, 2486, 2495, 3160
HpySE526I ACGT 4 cut(s) 1613, 1671, 2184, 2592
HspAI GCGC 1 cut(s) 3171
Kpn2I TCCGGA 2 cut(s) 2356, 2764
Ksp22I TGATCA 1 cut(s) 3051
LmnI GCTCC 4 cut(s) 941, 1513, 2167, 2305
Lsp1109I GCAGC 5 cut(s) 1569, 1775, 2171, 2492, 2857
LweI GCATC 9 cut(s) 338, 362, 748, 1217, 1665, 2291, 2567, 2928, 3158
MaeI CTAG 8 cut(s) 468, 666, 1437, 1845, 1976, 2549, 3009, 3147
MaeII ACGT 4 cut(s) 1613, 1671, 2184, 2592
MaeIII GTNAC 7 cut(s) 641, 921, 1166, 1181, 1544, 2648, 2905
MfeI CAATTG 2 cut(s) 76, 2490
MflI RGATCY 3 cut(s) 55, 67, 1439
MhlI GDGCHC 3 cut(s) 819, 1221, 2404
MlsI TGGCCA 2 cut(s) 1256, 2174
MluNI TGGCCA 2 cut(s) 1256, 2174
MlyI GAGTC 6 cut(s) 866, 1261, 1606, 2057, 2795, 3043
MmeI TCCRAC 1 cut(s) 528
Mox20I TGGCCA 2 cut(s) 1256, 2174
MroI TCCGGA 2 cut(s) 2356, 2764
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 2 cut(s) 1256, 2174
MseI TTAA 7 cut(s) 60, 225, 477, 1307, 1347, 1722, 3200
MslI CAYNNNNRTG 1 cut(s) 1939
Msp20I TGGCCA 2 cut(s) 1256, 2174
MspA1I CMGCKG 3 cut(s) 155, 602, 2848
MspCI CTTAAG 2 cut(s) 1306, 1721
MspI CCGG 7 cut(s) 35, 863, 1052, 2357, 2765, 2877, 3062
MspR9I CCNGG 5 cut(s) 36, 864, 1264, 2877, 3062
MunI CAATTG 2 cut(s) 76, 2490
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 1 cut(s) 1264
MvnI CGCG 1 cut(s) 3173
MwoI GCNNNNNNNGC 6 cut(s) 758, 1790, 2477, 2486, 2495, 3160
NciI CCSGG 4 cut(s) 36, 864, 2877, 3062
NcoI CCATGG 2 cut(s) 321, 1772
NdeI CATATG 1 cut(s) 315
NlaIV GGNNCC 8 cut(s) 69, 429, 1495, 1755, 2763, 2794, 2825, 3155
NmuCI GTSAC 4 cut(s) 641, 921, 1544, 2648
NspI RCATGY 4 cut(s) 664, 2400, 2607, 2749
PaeI GCATGC 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 2457
PagI TCATGA 1 cut(s) 1078
PciI ACATGT 1 cut(s) 2603
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 1263
PfoI TCCNGGA 1 cut(s) 1262
PkrI GCNGC 8 cut(s) 154, 1559, 1765, 2161, 2482, 2847, 2850, 3175
PleI GAGTC 6 cut(s) 866, 1261, 1605, 2056, 2794, 3042
PpsI GAGTC 6 cut(s) 866, 1261, 1605, 2056, 2794, 3042
PpuMI RGGWCCY 1 cut(s) 1753
PscI ACATGT 1 cut(s) 2603
Psp5II RGGWCCY 1 cut(s) 1753
Psp6I CCWGG 1 cut(s) 1262
PspFI CCCAGC 2 cut(s) 1047, 1710
PspGI CCWGG 1 cut(s) 1262
PspN4I GGNNCC 8 cut(s) 69, 429, 1495, 1755, 2763, 2794, 2825, 3155
PspPPI RGGWCCY 1 cut(s) 1753
PstI CTGCAG 1 cut(s) 2161
PstNI CAGNNNCTG 2 cut(s) 931, 2165
PsuI RGATCY 3 cut(s) 55, 67, 1439
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 5 cut(s) 257, 611, 1198, 1472, 1616
RsaNI GTAC 5 cut(s) 256, 610, 1197, 1471, 1615
RseI CAYNNNNRTG 1 cut(s) 1939
SaqAI TTAA 7 cut(s) 60, 225, 477, 1307, 1347, 1722, 3200
SatI GCNGC 8 cut(s) 153, 1558, 1764, 2160, 2481, 2846, 2849, 3174
SchI GAGTC 6 cut(s) 866, 1261, 1606, 2057, 2795, 3043
ScrFI CCNGG 5 cut(s) 36, 864, 1264, 2877, 3062
SduI GDGCHC 3 cut(s) 819, 1221, 2404
SfaNI GCATC 9 cut(s) 338, 362, 748, 1217, 1665, 2291, 2567, 2928, 3158
SfcI CTRYAG 1 cut(s) 2157
Sfr274I CTCGAG 1 cut(s) 2457
SinI GGWCC 5 cut(s) 428, 1054, 1522, 1753, 2513
SlaI CTCGAG 1 cut(s) 2457
SmiMI CAYNNNNRTG 1 cut(s) 1939
SmlI CTYRAG 6 cut(s) 340, 1306, 1363, 1721, 2457, 2618
SmoI CTYRAG 6 cut(s) 340, 1306, 1363, 1721, 2457, 2618
SphI GCATGC 1 cut(s) 664
SspI AATATT 1 cut(s) 128
SspMI CTAG 8 cut(s) 468, 666, 1437, 1845, 1976, 2549, 3009, 3147
StyD4I CCNGG 5 cut(s) 34, 862, 1262, 2875, 3060
StyI CCWWGG 6 cut(s) 146, 321, 782, 1447, 1772, 2730
TaaI ACNGT 5 cut(s) 432, 1006, 1544, 1688, 2226
TaiI ACGT 4 cut(s) 1616, 1674, 2187, 2595
TaqI TCGA 2 cut(s) 159, 2458
TatI WGTACW 2 cut(s) 255, 609
TauI GCSGC 3 cut(s) 155, 2851, 3176
Tru1I TTAA 7 cut(s) 60, 225, 477, 1307, 1347, 1722, 3200
Tru9I TTAA 7 cut(s) 60, 225, 477, 1307, 1347, 1722, 3200
TscAI CASTG 7 cut(s) 23, 646, 1019, 1843, 2331, 3033, 3114
TseFI GTSAC 4 cut(s) 641, 921, 1544, 2648
TseI GCWGC 5 cut(s) 1557, 1763, 2159, 2480, 2845
Tsp45I GTSAC 4 cut(s) 641, 921, 1544, 2648
TspGWI ACGGA 3 cut(s) 498, 1040, 1115
TspRI CASTG 7 cut(s) 23, 646, 1019, 1843, 2331, 3033, 3114
Van91I CCANNNNNTGG 1 cut(s) 1263
Vha464I CTTAAG 2 cut(s) 1306, 1721
VneI GTGCAC 1 cut(s) 2400
VpaK11BI GGWCC 5 cut(s) 428, 1054, 1522, 1753, 2513
XapI RAATTY 5 cut(s) 1087, 1117, 1630, 1911, 2702
XbaI TCTAGA 1 cut(s) 1436
XceI RCATGY 4 cut(s) 664, 2400, 2607, 2749
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 2457
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 8 cut(s) 468, 666, 1437, 1845, 1976, 2549, 3009, 3147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.