Rorug04G0314200

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007903
Physical Location & Seq
Forward (+)
48891991 .. 48895691
3701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0314200.1

Sequence Viewer

Length: 921 bp
ATGCAGAATCAGGAAGGGCTTCAAGTGCAAGTGGATGGGGAAGTGGAGTCTGGGCCGGCCCCCTCGGTTGCAACACCTGACTTTGATGAGTGGGCAAGCTTTGGGGACCAGGATATCATGCAGCAGCACTCTGCCATTCGCGCCGAGGAGGCCGAGAAAATTCCCTTCCTGGGGGACAAGGAACCACTCTCCTCCCTGGCAGCTGAGTATCAATCAGGCAGCGCTATTTTGTTGGAGAAAATAAAGGTTCTTGGAGAACAATATGCTGCCATTAGGAGAACCCGGGGCGACGGCAACTGCTTCTTCCGAAGCTTTATGTTTTCATACCTGGAGCATATTTTGGAATCACAAGATGTAAGTGAAGTTGATCGTATCAAGGCTAATGTTGAACAATGTAGAAAAACACTTCAGAGCTTGGGTTATGCAGACTTTACCTTTGAGGACTTCTTTTCGCTATTTCTTGAGCAACTGGATAGTGTTCTTCAAGGAAATGAAGAATCCATTAGTCATGATGAGCTCATACTTCGGAGCCGTGATCAGTCAGTATCCGACTATGTTGTGATGTTTTTCAGATTTGTTACCTCTGGTGAAATAAGGAAGCGCTCAGAGTTTTTTGAACCTTTTATATTGGGATTAACCAATGCAACCGTAGAGCAGTTTTGCAAGTCATCAGTGGAACCAATGGGTGAAGAAAGTGACCACGTGCACATCACTGCCCTGTCAGATGCACTGGGTGTGCCAATCCGTGTTGTGTACCTTGACCGCAGCTCATGTGATACTGGTGGTGTTAGCGTGAATCATCACGATTTCGTTCCTGCTGGCAATGATCTGCCAAATGCAAGCGATAGCTCTGAGAAAGTTAGTCCTTTTATTACCTTGCTATATCGCCCAGGTCACTACGATATTCTCTACCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

306

Amino Acids

34.32

Weight (kDa)

4.54

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C65 PF10275 50 - 305 1.1e-90 Peptidase C65 Otubain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 141
AciI CCGC 1 cut(s) 763
AcsI RAATTY 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 392
AcvI CACGTG 1 cut(s) 703
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 1 cut(s) 755
AfeI AGCGCT 2 cut(s) 223, 602
AfiI CCNNNNNNNGG 2 cut(s) 170, 171
AgsI TTSAA 4 cut(s) 23, 389, 485, 617
AjnI CCWGG 5 cut(s) 108, 168, 195, 327, 889
AluBI AGCT 7 cut(s) 99, 203, 312, 414, 517, 768, 849
AluI AGCT 7 cut(s) 99, 203, 312, 414, 517, 768, 849
Alw21I GWGCWC 2 cut(s) 519, 708
Alw44I GTGCAC 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 282
Aor51HI AGCGCT 2 cut(s) 223, 602
AoxI GGCC 3 cut(s) 53, 57, 150
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 6 cut(s) 121, 124, 200, 219, 266, 765
ApoI RAATTY 1 cut(s) 159
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 3 cut(s) 143, 224, 603
AspS9I GGNCC 3 cut(s) 53, 58, 106
AsuC2I CCSGG 2 cut(s) 283, 284
AsuHPI GGTGA 2 cut(s) 599, 698
AvaI CYCGRG 1 cut(s) 282
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 708
BanII GRGCYC 1 cut(s) 519
BbrPI CACGTG 1 cut(s) 703
Bbv12I GWGCWC 2 cut(s) 519, 708
BbvI GCAGC 6 cut(s) 133, 136, 212, 231, 253, 777
BccI CCATC 1 cut(s) 29
BceAI ACGGC 2 cut(s) 307, 516
BciT130I CCWGG 5 cut(s) 110, 170, 197, 329, 891
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 283, 284
BfoI RGCGCY 2 cut(s) 225, 604
BfuI GTATCC 1 cut(s) 556
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 6 cut(s) 122, 125, 201, 220, 267, 766
BlsI GCNGC 6 cut(s) 123, 126, 202, 221, 268, 767
Bme1390I CCNGG 7 cut(s) 110, 170, 197, 283, 284, 329, 891
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 282
BmgT120I GGNCC 3 cut(s) 53, 58, 106
BmiI GGNNCC 5 cut(s) 60, 107, 183, 530, 678
BmrFI CCNGG 7 cut(s) 110, 170, 197, 283, 284, 329, 891
BmrI ACTGGG 1 cut(s) 740
BmsI GCATC 1 cut(s) 715
BmuI ACTGGG 1 cut(s) 740
BpmI CTGGAG 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 283, 284
BsaAI YACGTR 1 cut(s) 703
BsaJI CCNNGG 7 cut(s) 63, 144, 169, 195, 282, 283, 889
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 171
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 474, 735, 784
Bse3DI GCAATG 1 cut(s) 829
BseBI CCWGG 5 cut(s) 110, 170, 197, 329, 891
BseDI CCNNGG 7 cut(s) 63, 144, 169, 195, 282, 283, 889
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 2 cut(s) 170, 171
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 3 cut(s) 195, 618, 843
BseNI ACTGG 3 cut(s) 474, 735, 784
BseRI GAGGAG 2 cut(s) 161, 181
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 6 cut(s) 133, 136, 212, 231, 253, 777
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 55, 59, 152
BsiHKAI GWGCWC 2 cut(s) 519, 708
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 2 cut(s) 56, 283
BslFI GGGAC 2 cut(s) 119, 188
BslI CCNNNNNNNGG 2 cut(s) 170, 171
BsmFI GGGAC 2 cut(s) 119, 188
BsnI GGCC 3 cut(s) 55, 59, 152
BsoBI CYCGRG 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 519, 708
Bsp143I GATC 3 cut(s) 367, 535, 826
BspACI CCGC 1 cut(s) 763
BspANI GGCC 3 cut(s) 55, 59, 152
BspCNI CTCAG 3 cut(s) 196, 617, 844
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 5 cut(s) 60, 107, 183, 530, 678
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 474, 735, 784
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 7 cut(s) 63, 144, 169, 195, 282, 283, 889
BssMI GATC 3 cut(s) 367, 535, 826
Bst2UI CCWGG 5 cut(s) 110, 170, 197, 329, 891
Bst4CI ACNGT 1 cut(s) 649
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 4 cut(s) 57, 97, 820, 841
BstDEI CTNAG 3 cut(s) 204, 604, 852
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 1 cut(s) 141
BstH2I RGCGCY 2 cut(s) 225, 604
BstHHI GCGC 3 cut(s) 143, 224, 603
BstKTI GATC 3 cut(s) 370, 538, 829
BstMBI GATC 3 cut(s) 367, 535, 826
BstMWI GCNNNNNNNGC 3 cut(s) 25, 140, 149
BstNI CCWGG 5 cut(s) 110, 170, 197, 329, 891
BstSCI CCNGG 7 cut(s) 108, 168, 195, 281, 282, 327, 889
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 6 cut(s) 133, 136, 212, 231, 253, 777
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 3 cut(s) 55, 59, 152
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 678, 711, 728
Cac8I GCNNGC 4 cut(s) 57, 97, 820, 841
CciI TCATGA 1 cut(s) 508
CfoI GCGC 3 cut(s) 143, 224, 603
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 53, 58, 106
Cfr9I CCCGGG 1 cut(s) 282
Csp6I GTAC 1 cut(s) 754
CviAII CATG 3 cut(s) 118, 509, 771
CviQI GTAC 1 cut(s) 754
DdeI CTNAG 3 cut(s) 204, 604, 852
DpnI GATC 3 cut(s) 369, 537, 828
DpnII GATC 3 cut(s) 367, 535, 826
DraIII CACNNNGTG 1 cut(s) 734
Ecl136II GAGCTC 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 519
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 106
Eco47III AGCGCT 2 cut(s) 223, 602
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 392
Eco72I CACGTG 1 cut(s) 703
Eco88I CYCGRG 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 517
EcoRII CCWGG 5 cut(s) 108, 168, 195, 327, 889
EcoRV GATATC 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 519
FaeI CATG 3 cut(s) 121, 512, 774
FaqI GGGAC 2 cut(s) 119, 188
FatI CATG 3 cut(s) 117, 508, 770
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 6 cut(s) 122, 125, 201, 220, 267, 766
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 519
FseI GGCCGGCC 1 cut(s) 59
Fsp4HI GCNGC 6 cut(s) 122, 125, 201, 220, 267, 766
GlaI GCGC 3 cut(s) 142, 223, 602
GluI GCNGC 6 cut(s) 122, 125, 201, 220, 267, 766
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 2 cut(s) 225, 604
HaeIII GGCC 3 cut(s) 55, 59, 152
HapII CCGG 2 cut(s) 56, 283
HhaI GCGC 3 cut(s) 143, 224, 603
Hin1II CATG 3 cut(s) 121, 512, 774
Hin6I GCGC 3 cut(s) 141, 222, 601
HinP1I GCGC 3 cut(s) 141, 222, 601
HindIII AAGCTT 2 cut(s) 97, 310
HinfI GANTC 5 cut(s) 7, 47, 344, 497, 796
HpaII CCGG 2 cut(s) 56, 283
HphI GGTGA 2 cut(s) 599, 698
Hpy166II GTNNAC 2 cut(s) 706, 754
Hpy188I TCNGA 8 cut(s) 308, 411, 528, 550, 572, 607, 724, 853
Hpy188III TCNNGA 4 cut(s) 11, 461, 509, 803
Hpy8I GTNNAC 2 cut(s) 706, 754
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 8, 175
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 1 cut(s) 702
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 140, 149
HpyF3I CTNAG 3 cut(s) 204, 604, 852
HpySE526I ACGT 1 cut(s) 702
Hsp92II CATG 3 cut(s) 121, 512, 774
HspAI GCGC 3 cut(s) 141, 222, 601
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 3 cut(s) 367, 535, 826
LmnI GCTCC 2 cut(s) 331, 528
Lsp1109I GCAGC 6 cut(s) 133, 136, 212, 231, 253, 777
LweI GCATC 1 cut(s) 715
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 3 cut(s) 577, 695, 893
MalI GATC 3 cut(s) 369, 537, 828
MboI GATC 3 cut(s) 367, 535, 826
MboII GAAGA 4 cut(s) 295, 473, 506, 701
MhlI GDGCHC 2 cut(s) 519, 708
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 213, 573
MnlI CCTC 6 cut(s) 73, 139, 142, 202, 433, 592
MroNI GCCGGC 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 56, 283
MspR9I CCNGG 7 cut(s) 110, 170, 197, 283, 284, 329, 891
MvaI CCWGG 5 cut(s) 110, 170, 197, 329, 891
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 3 cut(s) 25, 140, 149
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 2 cut(s) 283, 284
NdeII GATC 3 cut(s) 367, 535, 826
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 3 cut(s) 121, 512, 774
NlaIV GGNNCC 5 cut(s) 60, 107, 183, 530, 678
NmeAIII GCCGAG 2 cut(s) 169, 178
NmuCI GTSAC 2 cut(s) 695, 893
PagI TCATGA 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 4 cut(s) 7, 344, 497, 796
PkrI GCNGC 6 cut(s) 123, 126, 202, 221, 268, 767
PleI GAGTC 1 cut(s) 55
PmaCI CACGTG 1 cut(s) 703
PmlI CACGTG 1 cut(s) 703
PpsI GAGTC 1 cut(s) 55
Ppu21I YACGTR 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 519
Psp6I CCWGG 5 cut(s) 108, 168, 195, 327, 889
PspCI CACGTG 1 cut(s) 703
PspGI CCWGG 5 cut(s) 108, 168, 195, 327, 889
PspN4I GGNNCC 5 cut(s) 60, 107, 183, 530, 678
PspPI GGNCC 3 cut(s) 53, 58, 106
PvuII CAGCTG 1 cut(s) 203
RigI GGCCGGCC 1 cut(s) 59
RsaI GTAC 1 cut(s) 755
RsaNI GTAC 1 cut(s) 754
SacI GAGCTC 1 cut(s) 519
SaqAI TTAA 1 cut(s) 635
SatI GCNGC 6 cut(s) 122, 125, 201, 220, 267, 766
Sau3AI GATC 3 cut(s) 367, 535, 826
Sau96I GGNCC 3 cut(s) 53, 58, 106
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 7 cut(s) 110, 170, 197, 283, 284, 329, 891
SduI GDGCHC 2 cut(s) 519, 708
SfaNI GCATC 1 cut(s) 715
SinI GGWCC 1 cut(s) 106
SmaI CCCGGG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 1 cut(s) 159
SsiI CCGC 1 cut(s) 763
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 7 cut(s) 108, 168, 195, 281, 282, 327, 889
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 1 cut(s) 705
TasI AATT 1 cut(s) 159
TfiI GAWTC 4 cut(s) 7, 344, 497, 796
Tru1I TTAA 1 cut(s) 635
Tru9I TTAA 1 cut(s) 635
TscAI CASTG 3 cut(s) 678, 718, 735
TseFI GTSAC 2 cut(s) 695, 893
TseI GCWGC 6 cut(s) 121, 124, 200, 219, 266, 765
Tsp45I GTSAC 2 cut(s) 695, 893
TspDTI ATGAA 2 cut(s) 312, 507
TspGWI ACGGA 1 cut(s) 734
TspMI CCCGGG 1 cut(s) 282
TspRI CASTG 3 cut(s) 678, 718, 735
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 159
XmaI CCCGGG 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.