Rh4DG370700

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007903
Physical Location & Seq
Reverse (-)
58948743 .. 58955902
7160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG370700.1

Sequence Viewer

Length: 3123 bp
ATGTTTGTTCAAGATCCTTGCAGTATAAGCCGATTCGGGTCAATATTTAAGGACCTTAATTGGCATAGGCAGATTGATTTCAGCATTCTTTGCTCCTTTTTATTTCTACTCCATAAGACACTTGAACGGTTTCAATTTCATGTGATCCAGGTGTATGAACTTCATCGCCTGTACAGAATACAAAGGGACTTGATGGATGAAATCAAAAGAAAAGAACTGCATAGAAACCATATGCCCATGGAGGCATCATTATCCTCAAGTCCCTTAGCATCTCAAATTACTTCTGAACATACTCGGAAATGGCCTGATTCCAGCTTCCCTTTGGTGAACTCGGTTTATGCGGGACCGTCAAGTTCAGGTGTTGAAGGCATTCATTCTCAATCTAGTGCTATTAAAGGGAATGGGCGAAAAAATGGTCTATATCCGTGTCAAAATGGTATTAGTTCAAAAGATGTTGAGGTGTTGGATTCAAGACCTACAAAAGTGAGGAAAAAAATGTTTGACCTTCAACTTCCAGCTGATGTGTACATAGATTCTGAAGAAGGGGAAGAGTGCAGTGACGAGAAAGTTTCTTGCATGCCTAGTTGTCTGTCAACTAAAAATTGTAAAATCGCACCTGAGGGTGGTGGGAAGGTCTTTTTGGGTGATGGCGGAAAGTCTGACTTTGCAGGGGATGCGTTGAGATCAGAGAAATGTCCAAGGGGTGCAAATGGTTTTGCTGACTTGAATGAGCCCATTCAACCTGAAGAAGCAAGTGCTTCAGGATATAATGATCCTCCGGGCCATGACTCTTTTCGGGGGAAGATTCAAGGCCCTGATCTGCCTGCTAAGTCAAGGTCACAGCTTCTGGGTGATGGAGCAAAAAATGGGTGGTTTTCCCATGTTCTTGAATCAGGGCCAAATAAGAGCAACCTCAAAACTGTGTCCCAGTGTCCGCAAACGGAAAGGTTGCCTATATCATCCCAGCCGGTCCAAGCTTCTGTGAACAATGTTCATGAACAAAATTTCTATTTGACGGATAAAAGCAAGGTGAATTTGTGGAGAGAGAGGACAATTTCTGGTGTTGAGAGTTCCGAAAGAAGTAACGAGTTTTCCAGTAACAAGCACCCGAGTACCTTTGTGGCATCTAATGTGCCCAGTCCTTATCCGATTCTTCCTTCTTCTGATTTGGCCAAGTCCTGGACTCACTCGGTTTCTTCTTGGGAAAAGGCAGGCAGTAGCTTAAGCCAGAAGTCAATGTCAGTTCAAGCTCACCCATGTTTTAATTCATCTGCTACCTTGAGTAAGAGTTCCCAGTCATCGGTTCAGAGTAATGGGATTTTCGGAGACAGGTGGAATCTCAACAACAATTCTAGATCTAACCAAGGTTCAGGAAGTGAATTGCTGTACCAAAATGGGTTTCATCATGGTTCCTCTTCTGGGTCTAAGGAGCAACTGGTCCGCTTTCCTTCTCTCAACTGTGACTATCAGAGCAGCAGTAGCAATCATAATGGAGGTTCTGAACACTTGATGAGTCATGGTTCAGCAACGTACTACAAGGGTTCAAATTTCTTGGATGTGAAGTCTGCAAAGGAAGTAAACTTGAACGTGATGCTTTCAAACAGTTCATCTAATGAAGAATTACCCCAGCGAGGTCTTAAGATCATGGGTGGAGAACAAAAGCATGAGGACCCTCTTGCAGCCTTGCCATGGCTAAGAGCTAAGCCTGCTGGTAAGAATGAGTTTGCCAATGGTGGCGGAGTTTCTAAGACCAGTGAGCCTAGTATCTTTCAGTCTTCTGTGAATAATTTATCCAATAAAATTGAAGCTGGAAAGGGTTTCAATCAAATTTTTACTCAAAGTATAAAATCATTTTCATGCGGGAATGATGTTGAGGCGAGGAGGACTGAACTAGCTGATAGCCCGAGTAATAAAAAACTTCTTGGATTTCCTATTTTTGAGAAGTCTCAGCTTTTGAAGAATGAGTCCTTTTCTCTCACCTCCCCTTCTGTGTCTATTCCTCACCCATCGGAGAGTGAAGTGGAAAATAACAGAAGAAATAGATTGCTGGACATCAACTTGCCTTGTGATACTGCAGCTCCTGACTTGGCCAGAAAGAACGTAGCAGAAATTGTAGTGGTAGAAGACAGAAGGGATAAACAGTTTGGTAATTTCAGACATCACATTGACCTGAACTTCTGTATCAGCGAAGATGAAGCATCTTTGAAACCTTCTGCTCCAAGAGGCAGTATGAAGATTGCAGTGGAGATAGATTTGGAAGCCCCCATTTCTCCGGAGACTGATGATGAGGATGATGTCATTCATGGGGAAGCATGTGCACAAAAGCAGAACAAGATGTCTTTAGCATTACCACAGAAAAAAACTGAACCTTCTCGAGATGAGCTTGCAAGAGTAGCAGCAGAGGCAATTGTTGCCATTTCATCATGCGGTCCTCCTGATCCTTTGGATGAATCCTCTTGCAAACTAGCAGAAGCATCTTCAGTTGACCCTCTTATGTGGTTTGTGGACGTAGTTTCTACATGTGGGAATGATCTTGAGAACAAGTTTGACATTGTTCTCAGGAGTGACGATAGTGAAGGTATTGAAGAATCTTTAGTAGAAGAGTTTGATTACTTTGAATTCCTCACTTTGAAACTGAAGGAGACCAAGGAAGAAGATTACATGCCCAAGCCTTTGGTTCCGGAAAATATAAAGTTTGAAGAGTCAGGAACCAATTTACTATCAAATACGCCCCGAAGGGGCCAGTCAAGGAGAGGGAGGCAGCGGCGGGACTTCCAAAGGGATATCCTTCCCGGCCTCGTTTCTTTGTCAAGGCATGAGGTAACAGAAGATATCCAGACATTTGGAGGGTTGATGCGGGCAACAGGTCATCCTTCATGGCAATCGGGGTTGGCAAGAAGGAACTCAACAAGAAATGGTTGTGCTAGGGGCAGGCGACGAGCAGTGGCGAGTCCTTCACCCCCTATGATCACCATCCCGGTTTGCACTCCACCAATACAGCAGTTTAGTAATACCGAAATGGCACTGGAAGATAGAAGCCTAACAGGTTGGGGTAAGACTACTAGAAGGCCCCGTAGGCAAAGATGCGCGGCAGGTAATCCTCCCTCTCTTCCTTTAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

1040

Amino Acids

114.76

Weight (kDa)

6.4

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 113 - 1029 0.0 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3083
AccB7I CCANNNNNTGG 1 cut(s) 1179
AccII CGCG 1 cut(s) 3089
AccIII TCCGGA 2 cut(s) 2272, 2680
AclWI GGATC 4 cut(s) 8, 139, 767, 2432
AcoI YGGCCR 2 cut(s) 1170, 2088
AcsI RAATTY 5 cut(s) 1003, 1033, 1546, 1827, 2618
AcuI CTGAAG 5 cut(s) 558, 744, 765, 2463, 2657
AfaI GTAC 5 cut(s) 173, 527, 1114, 1388, 1532
AfiI CCNNNNNNNGG 7 cut(s) 623, 1179, 1300, 1419, 1631, 1689, 2738
AflII CTTAAG 2 cut(s) 1222, 1637
AflIII ACRYGT 1 cut(s) 2519
AjnI CCWGG 2 cut(s) 147, 1178
AjuI GAANNNNNNNTTGG 4 cut(s) 623, 655, 1383, 1415
Alw21I GWGCWC 1 cut(s) 2320
Alw26I GTCTC 4 cut(s) 1320, 1950, 2270, 2636
Alw44I GTGCAC 1 cut(s) 2316
AlwI GGATC 4 cut(s) 8, 139, 767, 2432
AlwNI CAGNNNCTG 2 cut(s) 847, 2081
Ama87I CYCGRG 3 cut(s) 1108, 1903, 2373
Aor13HI TCCGGA 2 cut(s) 2272, 2680
AoxI GGCC 9 cut(s) 302, 781, 811, 896, 1170, 2088, 2740, 2794, 3068
ApaLI GTGCAC 1 cut(s) 2316
ApeKI GCWGC 5 cut(s) 1473, 1679, 2075, 2396, 2761
ApoI RAATTY 5 cut(s) 1003, 1033, 1546, 1827, 2618
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
Asp700I GAANNNNTTC 2 cut(s) 129, 369
AspLEI GCGC 1 cut(s) 3089
AsuC2I CCSGG 3 cut(s) 780, 2793, 2978
AsuHPI GGTGA 9 cut(s) 337, 656, 863, 1042, 1244, 1969, 1994, 2949, 2962
AvaI CYCGRG 3 cut(s) 1108, 1903, 2373
AvaII GGWCC 6 cut(s) 52, 344, 970, 1438, 1669, 2429
AxyI CCTNAGG 1 cut(s) 618
BaeGI GKGCMC 2 cut(s) 1137, 2320
BaeI ACNNNNGTAYC 4 cut(s) 1096, 1129, 2164, 2197
BalI TGGCCA 2 cut(s) 1172, 2090
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 2 cut(s) 1767, 2130
Bbv12I GWGCWC 1 cut(s) 2320
BbvI GCAGC 5 cut(s) 1485, 1691, 2087, 2408, 2773
BccI CCATC 5 cut(s) 187, 641, 848, 2014, 2981
BciT130I CCWGG 2 cut(s) 149, 1180
BclI TGATCA 1 cut(s) 2967
BcnI CCSGG 3 cut(s) 780, 2793, 2978
BcoDI GTCTC 4 cut(s) 1320, 1950, 2270, 2636
BfaI CTAG 8 cut(s) 384, 582, 1353, 1761, 1892, 2465, 2925, 3063
BfmI CTRYAG 1 cut(s) 2073
BfrI CTTAAG 2 cut(s) 1222, 1637
BfuAI ACCTGC 1 cut(s) 3083
BglI GCCNNNNNGGC 1 cut(s) 3076
BglII AGATCT 1 cut(s) 1355
BisI GCNGC 7 cut(s) 1474, 1680, 2076, 2397, 2762, 2765, 3090
BlpI GCTNAGC 1 cut(s) 1701
BlsI GCNGC 7 cut(s) 1475, 1681, 2077, 2398, 2763, 2766, 3091
Bme1390I CCNGG 5 cut(s) 149, 780, 1180, 2793, 2978
Bme18I GGWCC 6 cut(s) 52, 344, 970, 1438, 1669, 2429
BmeT110I CYCGRG 3 cut(s) 1108, 1903, 2373
BmiI GGNNCC 7 cut(s) 345, 1411, 1671, 2679, 2710, 2741, 3071
BmrFI CCNGG 5 cut(s) 149, 780, 1180, 2793, 2978
BmrI ACTGGG 3 cut(s) 922, 1131, 1288
BmsI GCATC 9 cut(s) 254, 278, 664, 1133, 1581, 2207, 2483, 2844, 3074
BmuI ACTGGG 3 cut(s) 922, 1131, 1288
BpiI GAAGAC 2 cut(s) 1767, 2130
Bpu10I CCTNAGC 1 cut(s) 265
Bpu1102I GCTNAGC 1 cut(s) 1701
BpuEI CTTGAG 3 cut(s) 241, 1300, 2555
BpuMI CCSGG 3 cut(s) 780, 2793, 2978
BsaBI GATNNNNATC 1 cut(s) 2972
BsaI GGTCTC 1 cut(s) 2636
BsaJI CCNNGG 5 cut(s) 237, 698, 1363, 1688, 2646
BsaWI WCCGGW 2 cut(s) 2272, 2680
BsaXI ACNNNNNCTCC 2 cut(s) 2062, 2092
Bsc4I CCNNNNNNNGG 7 cut(s) 623, 1179, 1300, 1419, 1631, 1689, 2738
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 8 cut(s) 928, 1095, 1137, 1294, 1440, 1752, 2743, 3030
Bse21I CCTNAGG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 2972
BseAI TCCGGA 2 cut(s) 2272, 2680
BseBI CCWGG 2 cut(s) 149, 1180
BseDI CCNNGG 5 cut(s) 237, 698, 1363, 1688, 2646
BseGI GGATG 8 cut(s) 202, 679, 959, 1561, 2296, 2452, 2869, 2973
BseJI GATNNNNATC 1 cut(s) 2972
BseLI CCNNNNNNNGG 7 cut(s) 623, 1179, 1300, 1419, 1631, 1689, 2738
BseMII CTCAG 3 cut(s) 609, 1961, 2572
BseNI ACTGG 8 cut(s) 928, 1095, 1137, 1294, 1440, 1752, 2743, 3030
BseRI GAGGAG 1 cut(s) 1894
BseSI GKGCMC 2 cut(s) 1137, 2320
BseXI GCAGC 5 cut(s) 1485, 1691, 2087, 2408, 2773
BseYI CCCAGC 2 cut(s) 963, 1626
BsgI GTGCAG 1 cut(s) 574
Bsh1236I CGCG 1 cut(s) 3089
BshFI GGCC 9 cut(s) 304, 783, 813, 898, 1172, 2090, 2742, 2796, 3070
BsiHKAI GWGCWC 1 cut(s) 2320
BsiHKCI CYCGRG 3 cut(s) 1108, 1903, 2373
BsiSI CCGG 6 cut(s) 779, 968, 2273, 2681, 2793, 2978
BslFI GGGAC 5 cut(s) 200, 246, 357, 910, 2783
BslI CCNNNNNNNGG 7 cut(s) 623, 1179, 1300, 1419, 1631, 1689, 2738
BsmAI GTCTC 4 cut(s) 1320, 1950, 2270, 2636
BsmFI GGGAC 5 cut(s) 200, 246, 357, 910, 2783
BsmI GAATGC 2 cut(s) 84, 369
BsnI GGCC 9 cut(s) 304, 783, 813, 898, 1172, 2090, 2742, 2796, 3070
Bso31I GGTCTC 1 cut(s) 2636
BsoBI CYCGRG 3 cut(s) 1108, 1903, 2373
Bsp1286I GDGCHC 3 cut(s) 735, 1137, 2320
Bsp13I TCCGGA 2 cut(s) 2272, 2680
Bsp1407I TGTACA 2 cut(s) 171, 525
Bsp1720I GCTNAGC 1 cut(s) 1701
Bsp19I CCATGG 2 cut(s) 237, 1688
BspANI GGCC 9 cut(s) 304, 783, 813, 898, 1172, 2090, 2742, 2796, 3070
BspCNI CTCAG 3 cut(s) 610, 1960, 2571
BspEI TCCGGA 2 cut(s) 2272, 2680
BspFNI CGCG 1 cut(s) 3089
BspHI TCATGA 1 cut(s) 994
BspLI GGNNCC 7 cut(s) 345, 1411, 1671, 2679, 2710, 2741, 3071
BspMAI CTGCAG 1 cut(s) 2077
BspMI ACCTGC 1 cut(s) 3083
BspPI GGATC 4 cut(s) 8, 139, 767, 2432
BspTI CTTAAG 2 cut(s) 1222, 1637
BspTNI GGTCTC 1 cut(s) 2636
BsrFI RCCGGY 1 cut(s) 967
BsrGI TGTACA 2 cut(s) 171, 525
BsrI ACTGG 8 cut(s) 928, 1095, 1137, 1294, 1440, 1752, 2743, 3030
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 5 cut(s) 237, 698, 1363, 1688, 2646
BssT1I CCWWGG 5 cut(s) 237, 698, 1363, 1688, 2646
Bst2UI CCWGG 2 cut(s) 149, 1180
Bst4CI ACNGT 6 cut(s) 129, 348, 922, 1460, 1604, 2142
Bst6I CTCTTC 5 cut(s) 543, 1420, 2595, 2694, 3114
BstAFI CTTAAG 2 cut(s) 1222, 1637
BstAPI GCANNNNNTGC 3 cut(s) 90, 674, 2411
BstAUI TGTACA 2 cut(s) 171, 525
BstC8I GCNNGC 7 cut(s) 578, 825, 1213, 1707, 2385, 2859, 2933
BstDEI CTNAG 9 cut(s) 265, 618, 828, 1425, 1694, 1701, 1746, 1947, 2558
BstDSI CCRYGG 2 cut(s) 237, 1688
BstF5I GGATG 8 cut(s) 202, 679, 959, 1561, 2296, 2452, 2869, 2973
BstFNI CGCG 1 cut(s) 3089
BstHHI GCGC 1 cut(s) 3089
BstMAI GTCTC 4 cut(s) 1320, 1950, 2270, 2636
BstMWI GCNNNNNNNGC 9 cut(s) 27, 90, 674, 1479, 1706, 2393, 2402, 2411, 3076
BstNI CCWGG 2 cut(s) 149, 1180
BstNSI RCATGY 4 cut(s) 580, 2316, 2523, 2665
BstSCI CCNGG 5 cut(s) 147, 778, 1178, 2791, 2976
BstSFI CTRYAG 1 cut(s) 2073
BstSLI GKGCMC 2 cut(s) 1137, 2320
BstUI CGCG 1 cut(s) 3089
BstV1I GCAGC 5 cut(s) 1485, 1691, 2087, 2408, 2773
BstV2I GAAGAC 2 cut(s) 1767, 2130
BstX2I RGATCY 2 cut(s) 13, 1355
BstXI CCANNNNNNTGG 2 cut(s) 2674, 2843
BstYI RGATCY 2 cut(s) 13, 1355
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 9 cut(s) 304, 783, 813, 898, 1172, 2090, 2742, 2796, 3070
BtgI CCRYGG 2 cut(s) 237, 1688
BtgZI GCGATG 1 cut(s) 149
BtsCI GGATG 8 cut(s) 202, 679, 959, 1561, 2296, 2452, 2869, 2973
BtsI GCAGTG 3 cut(s) 562, 2247, 2949
BtsIMutI CAGTG 6 cut(s) 562, 935, 1759, 2247, 2949, 3023
BveI ACCTGC 1 cut(s) 3083
Cac8I GCNNGC 7 cut(s) 578, 825, 1213, 1707, 2385, 2859, 2933
CaiI CAGNNNCTG 2 cut(s) 847, 2081
CciI TCATGA 1 cut(s) 994
CfoI GCGC 1 cut(s) 3089
Cfr10I RCCGGY 1 cut(s) 967
Csp6I GTAC 5 cut(s) 172, 526, 1113, 1387, 1531
CviQI GTAC 5 cut(s) 172, 526, 1113, 1387, 1531
DdeI CTNAG 9 cut(s) 265, 618, 828, 1425, 1694, 1701, 1746, 1947, 2558
EaeI YGGCCR 2 cut(s) 1170, 2088
Eam1104I CTCTTC 5 cut(s) 543, 1420, 2595, 2694, 3114
EarI CTCTTC 5 cut(s) 543, 1420, 2595, 2694, 3114
EciI GGCGGA 2 cut(s) 666, 1752
Eco130I CCWWGG 5 cut(s) 237, 698, 1363, 1688, 2646
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 2636
Eco32I GATATC 2 cut(s) 2785, 2833
Eco47I GGWCC 6 cut(s) 52, 344, 970, 1438, 1669, 2429
Eco57I CTGAAG 5 cut(s) 558, 744, 765, 2463, 2657
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 3 cut(s) 1108, 1903, 2373
EcoO109I RGGNCCY 4 cut(s) 52, 812, 1669, 3069
EcoRI GAATTC 1 cut(s) 2618
EcoRII CCWGG 2 cut(s) 147, 1178
EcoRV GATATC 2 cut(s) 2785, 2833
EcoT14I CCWWGG 5 cut(s) 237, 698, 1363, 1688, 2646
EcoT38I GRGCYC 1 cut(s) 735
ErhI CCWWGG 5 cut(s) 237, 698, 1363, 1688, 2646
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 5 cut(s) 200, 246, 357, 910, 2783
FauI CCCGC 4 cut(s) 334, 1853, 2760, 2850
FauNDI CATATG 1 cut(s) 231
FbaI TGATCA 1 cut(s) 2967
Fnu4HI GCNGC 7 cut(s) 1474, 1680, 2076, 2397, 2762, 2765, 3090
FokI GGATG 8 cut(s) 209, 686, 946, 1568, 2303, 2459, 2856, 2960
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 7 cut(s) 1474, 1680, 2076, 2397, 2762, 2765, 3090
FspBI CTAG 8 cut(s) 384, 582, 1353, 1761, 1892, 2465, 2925, 3063
GlaI GCGC 1 cut(s) 3088
GluI GCNGC 7 cut(s) 1474, 1680, 2076, 2397, 2762, 2765, 3090
GsaI CCCAGC 2 cut(s) 967, 1630
HaeIII GGCC 9 cut(s) 304, 783, 813, 898, 1172, 2090, 2742, 2796, 3070
HapII CCGG 6 cut(s) 779, 968, 2273, 2681, 2793, 2978
HhaI GCGC 1 cut(s) 3089
Hin6I GCGC 1 cut(s) 3087
HinP1I GCGC 1 cut(s) 3087
HincII GTYRAC 2 cut(s) 594, 2485
HindII GTYRAC 2 cut(s) 594, 2485
HindIII AAGCTT 1 cut(s) 975
HpaII CCGG 6 cut(s) 779, 968, 2273, 2681, 2793, 2978
HphI GGTGA 9 cut(s) 337, 656, 863, 1042, 1244, 1969, 1994, 2949, 2962
Hpy166II GTNNAC 8 cut(s) 328, 526, 594, 985, 1579, 2318, 2485, 2506
Hpy8I GTNNAC 8 cut(s) 328, 526, 594, 985, 1579, 2318, 2485, 2506
Hpy99I CGWCG 1 cut(s) 2940
HpyCH4III ACNGT 6 cut(s) 129, 348, 922, 1460, 1604, 2142
HpyCH4IV ACGT 4 cut(s) 1529, 1587, 2100, 2508
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 90, 674, 1479, 1706, 2393, 2402, 2411, 3076
HpyF3I CTNAG 9 cut(s) 265, 618, 828, 1425, 1694, 1701, 1746, 1947, 2558
HpySE526I ACGT 4 cut(s) 1529, 1587, 2100, 2508
HspAI GCGC 1 cut(s) 3087
Kpn2I TCCGGA 2 cut(s) 2272, 2680
Ksp22I TGATCA 1 cut(s) 2967
LmnI GCTCC 5 cut(s) 98, 857, 1429, 2083, 2221
Lsp1109I GCAGC 5 cut(s) 1485, 1691, 2087, 2408, 2773
LweI GCATC 9 cut(s) 254, 278, 664, 1133, 1581, 2207, 2483, 2844, 3074
MaeI CTAG 8 cut(s) 384, 582, 1353, 1761, 1892, 2465, 2925, 3063
MaeII ACGT 4 cut(s) 1529, 1587, 2100, 2508
MaeIII GTNAC 7 cut(s) 557, 837, 1082, 1097, 1460, 2564, 2821
MfeI CAATTG 1 cut(s) 2406
MflI RGATCY 2 cut(s) 13, 1355
MhlI GDGCHC 3 cut(s) 735, 1137, 2320
MlsI TGGCCA 2 cut(s) 1172, 2090
MluNI TGGCCA 2 cut(s) 1172, 2090
MlyI GAGTC 6 cut(s) 782, 1177, 1522, 1973, 2711, 2959
MmeI TCCRAC 1 cut(s) 444
Mox20I TGGCCA 2 cut(s) 1172, 2090
MroI TCCGGA 2 cut(s) 2272, 2680
MroXI GAANNNNTTC 2 cut(s) 129, 369
MscI TGGCCA 2 cut(s) 1172, 2090
MseI TTAA 7 cut(s) 48, 57, 393, 1223, 1263, 1638, 3116
MslI CAYNNNNRTG 1 cut(s) 1855
Msp20I TGGCCA 2 cut(s) 1172, 2090
MspA1I CMGCKG 2 cut(s) 518, 2764
MspCI CTTAAG 2 cut(s) 1222, 1637
MspI CCGG 6 cut(s) 779, 968, 2273, 2681, 2793, 2978
MspR9I CCNGG 5 cut(s) 149, 780, 1180, 2793, 2978
MunI CAATTG 1 cut(s) 2406
Mva1269I GAATGC 2 cut(s) 84, 369
MvaI CCWGG 2 cut(s) 149, 1180
MvnI CGCG 1 cut(s) 3089
MwoI GCNNNNNNNGC 9 cut(s) 27, 90, 674, 1479, 1706, 2393, 2402, 2411, 3076
NciI CCSGG 3 cut(s) 780, 2793, 2978
NcoI CCATGG 2 cut(s) 237, 1688
NdeI CATATG 1 cut(s) 231
NlaIV GGNNCC 7 cut(s) 345, 1411, 1671, 2679, 2710, 2741, 3071
NmuCI GTSAC 4 cut(s) 557, 837, 1460, 2564
NspI RCATGY 4 cut(s) 580, 2316, 2523, 2665
PaeI GCATGC 1 cut(s) 580
PaeR7I CTCGAG 1 cut(s) 2373
PagI TCATGA 1 cut(s) 994
PciI ACATGT 1 cut(s) 2519
PctI GAATGC 2 cut(s) 84, 369
PdmI GAANNNNTTC 2 cut(s) 129, 369
PflMI CCANNNNNTGG 1 cut(s) 1179
PfoI TCCNGGA 1 cut(s) 1178
PkrI GCNGC 7 cut(s) 1475, 1681, 2077, 2398, 2763, 2766, 3091
PleI GAGTC 6 cut(s) 782, 1177, 1521, 1972, 2710, 2958
PpsI GAGTC 6 cut(s) 782, 1177, 1521, 1972, 2710, 2958
PpuMI RGGWCCY 2 cut(s) 52, 1669
PscI ACATGT 1 cut(s) 2519
Psp5II RGGWCCY 2 cut(s) 52, 1669
Psp6I CCWGG 2 cut(s) 147, 1178
PspFI CCCAGC 2 cut(s) 963, 1626
PspGI CCWGG 2 cut(s) 147, 1178
PspN4I GGNNCC 7 cut(s) 345, 1411, 1671, 2679, 2710, 2741, 3071
PspPPI RGGWCCY 2 cut(s) 52, 1669
PstI CTGCAG 1 cut(s) 2077
PstNI CAGNNNCTG 2 cut(s) 847, 2081
PsuI RGATCY 2 cut(s) 13, 1355
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 5 cut(s) 173, 527, 1114, 1388, 1532
RsaNI GTAC 5 cut(s) 172, 526, 1113, 1387, 1531
RseI CAYNNNNRTG 1 cut(s) 1855
SaqAI TTAA 7 cut(s) 48, 57, 393, 1223, 1263, 1638, 3116
SatI GCNGC 7 cut(s) 1474, 1680, 2076, 2397, 2762, 2765, 3090
SchI GAGTC 6 cut(s) 782, 1177, 1522, 1973, 2711, 2959
ScrFI CCNGG 5 cut(s) 149, 780, 1180, 2793, 2978
SduI GDGCHC 3 cut(s) 735, 1137, 2320
SfaNI GCATC 9 cut(s) 254, 278, 664, 1133, 1581, 2207, 2483, 2844, 3074
SfcI CTRYAG 1 cut(s) 2073
Sfr274I CTCGAG 1 cut(s) 2373
SinI GGWCC 6 cut(s) 52, 344, 970, 1438, 1669, 2429
SlaI CTCGAG 1 cut(s) 2373
SmiMI CAYNNNNRTG 1 cut(s) 1855
SmlI CTYRAG 6 cut(s) 256, 1222, 1279, 1637, 2373, 2534
SmoI CTYRAG 6 cut(s) 256, 1222, 1279, 1637, 2373, 2534
SphI GCATGC 1 cut(s) 580
SspI AATATT 1 cut(s) 45
SspMI CTAG 8 cut(s) 384, 582, 1353, 1761, 1892, 2465, 2925, 3063
StyD4I CCNGG 5 cut(s) 147, 778, 1178, 2791, 2976
StyI CCWWGG 5 cut(s) 237, 698, 1363, 1688, 2646
TaaI ACNGT 6 cut(s) 129, 348, 922, 1460, 1604, 2142
TaiI ACGT 4 cut(s) 1532, 1590, 2103, 2511
TaqI TCGA 1 cut(s) 2374
TatI WGTACW 2 cut(s) 171, 525
TauI GCSGC 2 cut(s) 2767, 3092
Tru1I TTAA 7 cut(s) 48, 57, 393, 1223, 1263, 1638, 3116
Tru9I TTAA 7 cut(s) 48, 57, 393, 1223, 1263, 1638, 3116
TscAI CASTG 6 cut(s) 562, 935, 1759, 2247, 2949, 3030
TseFI GTSAC 4 cut(s) 557, 837, 1460, 2564
TseI GCWGC 5 cut(s) 1473, 1679, 2075, 2396, 2761
Tsp45I GTSAC 4 cut(s) 557, 837, 1460, 2564
TspGWI ACGGA 3 cut(s) 414, 956, 1031
TspRI CASTG 6 cut(s) 562, 935, 1759, 2247, 2949, 3030
Van91I CCANNNNNTGG 1 cut(s) 1179
Vha464I CTTAAG 2 cut(s) 1222, 1637
VneI GTGCAC 1 cut(s) 2316
VpaK11BI GGWCC 6 cut(s) 52, 344, 970, 1438, 1669, 2429
XapI RAATTY 5 cut(s) 1003, 1033, 1546, 1827, 2618
XbaI TCTAGA 1 cut(s) 1352
XceI RCATGY 4 cut(s) 580, 2316, 2523, 2665
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 2373
XmnI GAANNNNTTC 2 cut(s) 129, 369
XspI CTAG 8 cut(s) 384, 582, 1353, 1761, 1892, 2465, 2925, 3063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.