Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0010823

Physical Location & Seq

Genomic Coordinates Reverse (-)

4564680 .. 4565840

Gene Structure 1161 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGAAGCGACGCAAGTTCGATTCCTTGCTGCCGCCGAGTCGACTCAGAATGTTTCAGGTTTTATTTGGGGCAGTGTTCGTCTACTTGCTCCTGATCAGCTTCAAAATACCCCATGTTTTGAAATCCGGGTTCGGGTCGTCGGGTTTGGACGACGGGCTGCTCGAGACACTGGAGGACGCATTTCCCATGACGTTTTGGCTCGAGAGCGAGGAGGAAATGGGCGAGAGGGACGCCCCGAGCAGGCCGGCGGGGGACACGTTTCGGGTCTCCGGCGGCTCACCGTACCGGACTCCCCAGCGGCAAATGCGGGAGGTCAAGAAAGTTTCCGGCTTGGTGTTCGAGGACACTCTGTTCGGCGGCAATGTCAGTAAGGACCAAGTCTCCGAGCTTCACAGAACTACCAAGAGCGCTTGGGCAGCTGGGAAGGAGCTGTGGGCGGAGCTTGAGGCCAGAAAGCTTGAGTCTGACCTGGAGACCAAGCCCGAGAACCGCTCCGATCCGTGCCCGGGCTCGTTTTTTCTGTCCGGGTCCCAATTCGAGTCCCTGAAACGGGTAATGGTTATTCCCTGCGGGATGACTCTCGGGTCTCACATAACGGTGGTGGGCACGCCGAGGTGGGCCCACTCGGAGTACGACCCGAAGATTGCGATACTGAAGGAGGGGGACGAGGCGGTGATGGTCTCTCAGTTTATGATGGAGTTGCAGGGTTTGAAGAACGTGGAGGGGGAGGACCCGCCGAGGATACTGCATTTCAATCCGAGGTTGAAGGGGGATTGGAGTGGGAAGCCGGTGATCGAGCAGAACACTTGTTACCGGATGCAATGGGGGTCGGCCCTCCGCTGCGAGGGGTGGAAGTCCCTGGCTGATGAAGATACCTTTGATGGACAGGTGAAATGTGAGAAATGGATTCGTGACGATGACAATAAGTCGGAAGAATTGAAGGTCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006029 GO:0006464 GO:0006486 GO:0006493 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008378 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009507 GO:0009536 GO:0009791 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010384 GO:0010404 GO:0010405 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018208 GO:0018258 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044036 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048354 GO:0048359 GO:0048519 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0061458 GO:0065007 GO:0070085 GO:0071554 GO:0071704 GO:1900055 GO:1900056 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1905622 GO:1990714 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal-bind_lectin Galactosyl_T

Protein Analysis

317

Amino Acids

35.76

Weight (kDa)

5.48

Isoelectric Point (pI)

37.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal-bind_lectin	PF00337	185 - 300	2.7e-12	Galactoside-binding lectin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27120 AT1G27120
fragaria_vesca	FvH4_4g35520 FvH4_4g35520 FvH4_4g35520
malus_domestica	MD13G1082500.v1.1 MD16G1081600.v1.1
prunus_persica	Prupe.1G264500_v2.0.a1 Prupe.1G264500_v2.0.a1
pyrus_communis	pycom16g07040
rosa_chinensis	RchiOBHm_Chr4g0445171
rosa_laevigata	RLG00000005761
rosa_multiflora	Rmu_sc0000050.1_g000038
rosa_roxburghii	Rroxscaffold_5G00385680
rosa_rugosa	Rorug04G0359600
rosa_samantha	Rh4AG420400 Rh4BG432300 Rh4CG446900 Rh4DG428400
rosa_wichuraiana	Rw4G036050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	583
AccBSI	CCGCTC	1 cut(s)	492
AccI	GTMKAC	2 cut(s)	40, 81
AclWI	GGATC	1 cut(s)	491
AcuI	CTGAAG	1 cut(s)	674
AcyI	GRCGYC	1 cut(s)	231
AfaI	GTAC	2 cut(s)	284, 632
AfeI	AGCGCT	1 cut(s)	409
AfiI	CCNNNNNNNGG	6 cut(s)	132, 240, 506, 549, 550, 844
AflIII	ACRYGT	1 cut(s)	255
AgsI	TTSAA	6 cut(s)	103, 121, 712, 754, 766, 940
AhdI	GACNNNNNGTC	1 cut(s)	925
AjnI	CCWGG	2 cut(s)	468, 858
AloI	GAACNNNNNNTCC	2 cut(s)	335, 367
AluBI	AGCT	6 cut(s)	99, 388, 419, 430, 442, 457
AluI	AGCT	6 cut(s)	99, 388, 419, 430, 442, 457
Alw26I	GTCTC	6 cut(s)	158, 271, 385, 467, 591, 685
AlwI	GGATC	1 cut(s)	491
Ama87I	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
Aor51HI	AGCGCT	1 cut(s)	409
AoxI	GGCC	4 cut(s)	242, 447, 618, 831
ApaI	GGGCCC	1 cut(s)	622
ApeKI	GCWGC	4 cut(s)	28, 157, 416, 840
ArsI	GACNNNNNNTTYG	2 cut(s)	335, 367
AspLEI	GCGC	1 cut(s)	410
AspS9I	GGNCC	6 cut(s)	373, 528, 618, 619, 730, 832
AsuC2I	CCSGG	4 cut(s)	127, 506, 507, 526
AsuHPI	GGTGA	4 cut(s)	270, 685, 802, 901
AvaI	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
AvaII	GGWCC	3 cut(s)	373, 528, 730
BaeGI	GKGCMC	3 cut(s)	506, 608, 622
BanII	GRGCYC	2 cut(s)	512, 622
BbvI	GCAGC	4 cut(s)	15, 144, 428, 827
BccI	CCATC	3 cut(s)	670, 688, 875
BcgI	CGANNNNNNTGC	2 cut(s)	727, 761
BciT130I	CCWGG	2 cut(s)	470, 860
BciVI	GTATCC	1 cut(s)	735
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	4 cut(s)	127, 506, 507, 526
BcoDI	GTCTC	6 cut(s)	158, 271, 385, 467, 591, 685
BfoI	RGCGCY	1 cut(s)	411
BfuI	GTATCC	1 cut(s)	735
BisI	GCNGC	8 cut(s)	29, 32, 158, 274, 299, 358, 417, 841
BlsI	GCNGC	8 cut(s)	30, 33, 159, 275, 300, 359, 418, 842
Bme1390I	CCNGG	6 cut(s)	127, 470, 506, 507, 526, 860
Bme18I	GGWCC	3 cut(s)	373, 528, 730
BmeRI	GACNNNNNGTC	1 cut(s)	925
BmeT110I	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
BmgT120I	GGNCC	6 cut(s)	373, 528, 618, 619, 730, 832
BmiI	GGNNCC	4 cut(s)	529, 530, 620, 732
BmrFI	CCNGG	6 cut(s)	127, 470, 506, 507, 526, 860
BmsI	GCATC	1 cut(s)	807
BplI	GAGNNNNNCTC	2 cut(s)	476, 508
BpmI	CTGGAG	2 cut(s)	191, 491
BpuEI	CTTGAG	2 cut(s)	464, 479
BpuMI	CCSGG	4 cut(s)	127, 506, 507, 526
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	4 cut(s)	271, 467, 591, 685
BsaJI	CCNNGG	5 cut(s)	505, 611, 737, 758, 858
BsaWI	WCCGGW	2 cut(s)	285, 813
BsaXI	ACNNNNNCTCC	2 cut(s)	365, 395
Bsc4I	CCNNNNNNNGG	6 cut(s)	132, 240, 506, 549, 550, 844
Bse118I	RCCGGY	2 cut(s)	244, 787
Bse1I	ACTGG	1 cut(s)	174
Bse3DI	GCAATG	2 cut(s)	367, 827
BseBI	CCWGG	2 cut(s)	470, 860
BseDI	CCNNGG	5 cut(s)	505, 611, 737, 758, 858
BseGI	GGATG	2 cut(s)	579, 822
BseLI	CCNNNNNNNGG	6 cut(s)	132, 240, 506, 549, 550, 844
BseMI	GCAATG	2 cut(s)	367, 827
BseMII	CTCAG	2 cut(s)	58, 698
BseNI	ACTGG	1 cut(s)	174
BseRI	GAGGAG	1 cut(s)	224
BseSI	GKGCMC	3 cut(s)	506, 608, 622
BseXI	GCAGC	4 cut(s)	15, 144, 428, 827
BseYI	CCCAGC	2 cut(s)	294, 419
BshFI	GGCC	4 cut(s)	244, 449, 620, 833
BsiHKCI	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
BsiSI	CCGG	9 cut(s)	126, 245, 270, 286, 327, 506, 525, 788, 814
BslFI	GGGAC	6 cut(s)	242, 266, 514, 526, 677, 841
BslI	CCNNNNNNNGG	6 cut(s)	132, 240, 506, 549, 550, 844
BsmAI	GTCTC	6 cut(s)	158, 271, 385, 467, 591, 685
BsmFI	GGGAC	6 cut(s)	242, 266, 514, 526, 677, 841
BsnI	GGCC	4 cut(s)	244, 449, 620, 833
Bso31I	GGTCTC	4 cut(s)	271, 467, 591, 685
BsoBI	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
Bsp120I	GGGCCC	1 cut(s)	618
Bsp1286I	GDGCHC	4 cut(s)	506, 512, 608, 622
Bsp143I	GATC	3 cut(s)	93, 496, 792
BspANI	GGCC	4 cut(s)	244, 449, 620, 833
BspCNI	CTCAG	2 cut(s)	57, 697
BspLI	GGNNCC	4 cut(s)	529, 530, 620, 732
BspPI	GGATC	1 cut(s)	491
BspTNI	GGTCTC	4 cut(s)	271, 467, 591, 685
BsrBI	CCGCTC	1 cut(s)	492
BsrDI	GCAATG	2 cut(s)	367, 827
BsrFI	RCCGGY	2 cut(s)	244, 787
BsrI	ACTGG	1 cut(s)	174
BssAI	RCCGGY	2 cut(s)	244, 787
BssECI	CCNNGG	5 cut(s)	505, 611, 737, 758, 858
BssMI	GATC	3 cut(s)	93, 496, 792
BssNI	GRCGYC	1 cut(s)	231
Bst2UI	CCWGG	2 cut(s)	470, 860
Bst4CI	ACNGT	2 cut(s)	282, 598
BstACI	GRCGYC	1 cut(s)	231
BstC8I	GCNNGC	3 cut(s)	242, 246, 608
BstDEI	CTNAG	2 cut(s)	44, 684
BstF5I	GGATG	2 cut(s)	579, 822
BstH2I	RGCGCY	1 cut(s)	411
BstHHI	GCGC	1 cut(s)	410
BstKTI	GATC	3 cut(s)	96, 499, 795
BstMAI	GTCTC	6 cut(s)	158, 271, 385, 467, 591, 685
BstMBI	GATC	3 cut(s)	93, 496, 792
BstMWI	GCNNNNNNNGC	2 cut(s)	304, 416
BstNI	CCWGG	2 cut(s)	470, 860
BstSCI	CCNGG	6 cut(s)	125, 468, 504, 505, 524, 858
BstSLI	GKGCMC	3 cut(s)	506, 608, 622
BstV1I	GCAGC	4 cut(s)	15, 144, 428, 827
BsuI	GTATCC	1 cut(s)	735
BsuRI	GGCC	4 cut(s)	244, 449, 620, 833
BtsCI	GGATG	2 cut(s)	579, 822
BtsI	GCAGTG	1 cut(s)	78
BtsIMutI	CAGTG	2 cut(s)	78, 167
Cac8I	GCNNGC	3 cut(s)	242, 246, 608
CfoI	GCGC	1 cut(s)	410
Cfr10I	RCCGGY	2 cut(s)	244, 787
Cfr13I	GGNCC	6 cut(s)	373, 528, 618, 619, 730, 832
Cfr9I	CCCGGG	1 cut(s)	505
CseI	GACGC	3 cut(s)	18, 185, 239
Csp6I	GTAC	2 cut(s)	283, 631
CviAII	CATG	3 cut(s)	113, 187, 948
CviQI	GTAC	2 cut(s)	283, 631
DdeI	CTNAG	2 cut(s)	44, 684
DpnI	GATC	3 cut(s)	95, 498, 794
DpnII	GATC	3 cut(s)	93, 496, 792
DrdI	GACNNNNNNGTC	1 cut(s)	583
DriI	GACNNNNNGTC	1 cut(s)	925
DseDI	GACNNNNNNGTC	1 cut(s)	583
Eam1105I	GACNNNNNGTC	1 cut(s)	925
EciI	GGCGGA	1 cut(s)	452
Eco24I	GRGCYC	2 cut(s)	512, 622
Eco31I	GGTCTC	4 cut(s)	271, 467, 591, 685
Eco47I	GGWCC	3 cut(s)	373, 528, 730
Eco47III	AGCGCT	1 cut(s)	409
Eco57I	CTGAAG	1 cut(s)	674
Eco88I	CYCGRG	6 cut(s)	161, 200, 235, 482, 505, 581
EcoO109I	RGGNCCY	2 cut(s)	528, 730
EcoRII	CCWGG	2 cut(s)	468, 858
EcoT38I	GRGCYC	2 cut(s)	512, 622
FaeI	CATG	3 cut(s)	116, 190, 951
FaiI	YATR	5 cut(s)	114, 188, 593, 692, 949
FaqI	GGGAC	6 cut(s)	242, 266, 514, 526, 677, 841
FatI	CATG	3 cut(s)	112, 186, 947
FauI	CCCGC	4 cut(s)	241, 300, 563, 741
FbaI	TGATCA	1 cut(s)	93
FblI	GTMKAC	2 cut(s)	40, 81
Fnu4HI	GCNGC	8 cut(s)	29, 32, 158, 274, 299, 358, 417, 841
FokI	GGATG	2 cut(s)	586, 829
FriOI	GRGCYC	2 cut(s)	512, 622
Fsp4HI	GCNGC	8 cut(s)	29, 32, 158, 274, 299, 358, 417, 841
GlaI	GCGC	1 cut(s)	409
GluI	GCNGC	8 cut(s)	29, 32, 158, 274, 299, 358, 417, 841
GsaI	CCCAGC	2 cut(s)	298, 423
GsuI	CTGGAG	2 cut(s)	191, 491
HaeII	RGCGCY	1 cut(s)	411
HaeIII	GGCC	4 cut(s)	244, 449, 620, 833
HapII	CCGG	9 cut(s)	126, 245, 270, 286, 327, 506, 525, 788, 814
HgaI	GACGC	3 cut(s)	18, 185, 239
HhaI	GCGC	1 cut(s)	410
Hin1I	GRCGYC	1 cut(s)	231
Hin1II	CATG	3 cut(s)	116, 190, 951
Hin6I	GCGC	1 cut(s)	408
HinP1I	GCGC	1 cut(s)	408
HincII	GTYRAC	1 cut(s)	41
HindII	GTYRAC	1 cut(s)	41
HindIII	AAGCTT	1 cut(s)	455
HinfI	GANTC	8 cut(s)	20, 37, 42, 289, 461, 539, 577, 907
HpaII	CCGG	9 cut(s)	126, 245, 270, 286, 327, 506, 525, 788, 814
HphI	GGTGA	4 cut(s)	270, 685, 802, 901
Hpy166II	GTNNAC	2 cut(s)	41, 82
Hpy188I	TCNGA	7 cut(s)	47, 385, 466, 496, 628, 759, 931
Hpy188III	TCNNGA	5 cut(s)	91, 163, 202, 316, 911
Hpy8I	GTNNAC	2 cut(s)	41, 82
Hpy99I	CGWCG	3 cut(s)	12, 142, 155
HpyAV	CCTTC	4 cut(s)	418, 649, 760, 934
HpyCH4III	ACNGT	2 cut(s)	282, 598
HpyCH4IV	ACGT	3 cut(s)	191, 257, 717
HpyCH4V	TGCA	3 cut(s)	703, 748, 820
HpyF10VI	GCNNNNNNNGC	2 cut(s)	304, 416
HpyF3I	CTNAG	2 cut(s)	44, 684
HpySE526I	ACGT	3 cut(s)	191, 257, 717
Hsp92I	GRCGYC	1 cut(s)	231
Hsp92II	CATG	3 cut(s)	116, 190, 951
HspAI	GCGC	1 cut(s)	408
KflI	GGGWCCC	1 cut(s)	528
KroI	GCCGGC	1 cut(s)	244
KroNI	GCCGGC	1 cut(s)	246
Ksp22I	TGATCA	1 cut(s)	93
Kzo9I	GATC	3 cut(s)	93, 496, 792
LmnI	GCTCC	4 cut(s)	93, 427, 439, 497
Lsp1109I	GCAGC	4 cut(s)	15, 144, 428, 827
LweI	GCATC	1 cut(s)	807
MaeII	ACGT	3 cut(s)	191, 257, 717
MaeIII	GTNAC	3 cut(s)	809, 911, 943
MalI	GATC	3 cut(s)	95, 498, 794
MbiI	CCGCTC	1 cut(s)	492
MboI	GATC	3 cut(s)	93, 496, 792
MboII	GAAGA	4 cut(s)	652, 724, 881, 944
MhlI	GDGCHC	4 cut(s)	506, 512, 608, 622
MluCI	AATT	2 cut(s)	533, 935
MlyI	GAGTC	6 cut(s)	36, 46, 283, 470, 548, 571
MmeI	TCCRAC	1 cut(s)	909
MroNI	GCCGGC	1 cut(s)	244
MslI	CAYNNNNRTG	1 cut(s)	596
MspA1I	CMGCKG	3 cut(s)	298, 419, 840
MspI	CCGG	9 cut(s)	126, 245, 270, 286, 327, 506, 525, 788, 814
MspR9I	CCNGG	6 cut(s)	127, 470, 506, 507, 526, 860
MvaI	CCWGG	2 cut(s)	470, 860
MwoI	GCNNNNNNNGC	2 cut(s)	304, 416
NaeI	GCCGGC	1 cut(s)	246
NciI	CCSGG	4 cut(s)	127, 506, 507, 526
NdeII	GATC	3 cut(s)	93, 496, 792
NgoMIV	GCCGGC	1 cut(s)	244
NlaIII	CATG	3 cut(s)	116, 190, 951
NlaIV	GGNNCC	4 cut(s)	529, 530, 620, 732
NmeAIII	GCCGAG	3 cut(s)	60, 636, 762
NmuCI	GTSAC	2 cut(s)	911, 943
PaeR7I	CTCGAG	2 cut(s)	161, 200
PcsI	WCGNNNNNNNCGW	1 cut(s)	159
PdiI	GCCGGC	1 cut(s)	246
PfeI	GAWTC	2 cut(s)	20, 907
PflFI	GACNNNGTC	1 cut(s)	377
PkrI	GCNGC	8 cut(s)	30, 33, 159, 275, 300, 359, 418, 842
PleI	GAGTC	6 cut(s)	36, 45, 283, 469, 547, 571
PpsI	GAGTC	6 cut(s)	36, 45, 283, 469, 547, 571
PpuMI	RGGWCCY	2 cut(s)	528, 730
Psp5II	RGGWCCY	2 cut(s)	528, 730
Psp6I	CCWGG	2 cut(s)	468, 858
PspFI	CCCAGC	2 cut(s)	294, 419
PspGI	CCWGG	2 cut(s)	468, 858
PspN4I	GGNNCC	4 cut(s)	529, 530, 620, 732
PspOMI	GGGCCC	1 cut(s)	618
PspPI	GGNCC	6 cut(s)	373, 528, 618, 619, 730, 832
PspPPI	RGGWCCY	2 cut(s)	528, 730
PsrI	GAACNNNNNNTAC	2 cut(s)	794, 826
PsyI	GACNNNGTC	1 cut(s)	377
PvuII	CAGCTG	1 cut(s)	419
RsaI	GTAC	2 cut(s)	284, 632
RsaNI	GTAC	2 cut(s)	283, 631
RseI	CAYNNNNRTG	1 cut(s)	596
SalI	GTCGAC	1 cut(s)	39
SatI	GCNGC	8 cut(s)	29, 32, 158, 274, 299, 358, 417, 841
Sau3AI	GATC	3 cut(s)	93, 496, 792
Sau96I	GGNCC	6 cut(s)	373, 528, 618, 619, 730, 832
SchI	GAGTC	6 cut(s)	36, 46, 283, 470, 548, 571
ScrFI	CCNGG	6 cut(s)	127, 470, 506, 507, 526, 860
SduI	GDGCHC	4 cut(s)	506, 512, 608, 622
SfaNI	GCATC	1 cut(s)	807
Sfr274I	CTCGAG	2 cut(s)	161, 200
SinI	GGWCC	3 cut(s)	373, 528, 730
SlaI	CTCGAG	2 cut(s)	161, 200
SmaI	CCCGGG	1 cut(s)	507
SmiMI	CAYNNNNRTG	1 cut(s)	596
SmlI	CTYRAG	4 cut(s)	161, 200, 443, 458
SmoI	CTYRAG	4 cut(s)	161, 200, 443, 458
SrfI	GCCCGGGC	1 cut(s)	507
Sse9I	AATT	2 cut(s)	533, 935
StyD4I	CCNGG	6 cut(s)	125, 468, 504, 505, 524, 858
TaaI	ACNGT	2 cut(s)	282, 598
TaiI	ACGT	3 cut(s)	194, 260, 720
TaqI	TCGA	7 cut(s)	18, 40, 162, 201, 339, 537, 795
TasI	AATT	2 cut(s)	533, 935
TauI	GCSGC	4 cut(s)	34, 276, 301, 360
TfiI	GAWTC	2 cut(s)	20, 907
TscAI	CASTG	2 cut(s)	78, 174
TseFI	GTSAC	2 cut(s)	911, 943
TseI	GCWGC	4 cut(s)	28, 157, 416, 840
Tsp45I	GTSAC	2 cut(s)	911, 943
TspDTI	ATGAA	2 cut(s)	17, 882
TspGWI	ACGGA	1 cut(s)	489
TspMI	CCCGGG	1 cut(s)	505
TspRI	CASTG	2 cut(s)	78, 174
Tth111I	GACNNNGTC	1 cut(s)	377
VpaK11BI	GGWCC	3 cut(s)	373, 528, 730
XhoI	CTCGAG	2 cut(s)	161, 200
XmaI	CCCGGG	1 cut(s)	505
XmiI	GTMKAC	2 cut(s)	40, 81

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.