pycom16g08350

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013258
Physical Location & Seq
Reverse (-)
5559874 .. 5561634
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGTATAAGCAAGTGTATCGTTTTATGCCCAAATTACATGTTGAGCCCAAAAATTCACTCCCCTGTCGATTTTCATTTGCAGCAAAAACCCCTTGGATCACTCTCTTCTCTCTCTCGCAAAACCCTAATTTCAGAGGTCCAATGGCGCTGCAACAGTTCGGGCGGACGAAGAGCGTGACGAAGCGGTCGAGCAAGTACTTGGAGGAGGCTCTGTACCTGAGGCTGTTCAAAGACGGCGGCTCCGAGGTGAGCGTTCGGCAGCAGCTCAATGAAGTCCTCAAGAGCCGCAAGCGAGTTTACAAATGGGAGGTCGGCGACACTCTCAAGAAGCTTCGCGAACGCAAGCTTTACTACCCTGCTCTCAAGATGCTAAGCTGTTTAACATTACAGCTCTCGGAAGCTATGGATAGAAGAGGAATGAACAAGACAGTCAGTGATCAGGCAATACATCTTGATCTTGTTGCTAAAGTCCGAGGCATTCTTGCTGCGGAAAGTTATTTCATTGATCTTCCTGAATCAGCAAAGACCCATCTTTGTTACGGGGCCCTGCTCAACTGTTACTGTAAGGAATTGATGACTGAAAAAGCCGAAGCCCTTATAGAAAAGATGAAGGCTCTTAACCTACCTTTAACCTCCATGCCTTATAACAGCCTTATGACCCTTTACACTAAAACTGGAAAGCCGGATAAGATCCCAGCCATCTTACAAGAAATGAAGGCCTGTAATGTCATGCTCGATTCCTATTCATACAATGTCTGGATGCGGGCACTGGCTGCTGTTAATGATATTTCTGGGGCTGAAAGGGTTATTGATGAGATGAAGAGGGATGGTCGAGTTGCCAGTGATTGGACCACGTATAGCAACATAGCTTCAATTTTTGTTGAAGCTGGCATGTTTGAGAAGGCAGAAAATGCGCTTAAAGAATTGGAGAAGAAAAATTTGCACCGAGATCGATCTGCTTTCCCATTCCTCATTACATTGTATGGTAAAACAGGGAACCTGCGTGAAATTTATCGGGTATGGCGTTCCTTGAGGCTGGCTTTCCCTAAAACTGCAAATATCAGCTATCTGAATATCGATCCAGGTGTTGGTCAACTTGAATGA

Protein Analysis

368

Amino Acids

42.14

Weight (kDa)

9.55

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 215 - 258 1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60770
fragaria_vesca FvH4_4g12450
malus_domestica MD13G1097100.v1.1 MD16G1098000.v1.1
prunus_persica Prupe.1G248500_v2.0.a1
pyrus_communis pycom13g08480 pycom16g08350
rosa_chinensis RchiOBHm_Chr4g0410731
rosa_laevigata RLG00000008406
rosa_multiflora Rmu_sc0001967.1_g000008 Rmu_ssc0000072.1_g000002
rosa_roxburghii Rroxscaffold_5G00355480
rosa_rugosa Rorug04G0111500
rosa_samantha Rh4AG162600 Rh4BG161400 Rh4CG175500 Rh4DG158200
rosa_wichuraiana Rw4G013540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AasI GACNNNNNNGTC 1 cut(s) 184
Acc36I ACCTGC 1 cut(s) 1008
AccB7I CCANNNNNTGG 2 cut(s) 846, 1088
AccII CGCG 1 cut(s) 336
AciI CCGC 6 cut(s) 163, 184, 237, 286, 488, 763
AclWI GGATC 3 cut(s) 104, 685, 1073
AcsI RAATTY 3 cut(s) 52, 937, 1008
AfaI GTAC 2 cut(s) 197, 215
AfiI CCNNNNNNNGG 2 cut(s) 846, 1088
AflIII ACRYGT 1 cut(s) 37
AgsI TTSAA 4 cut(s) 229, 873, 884, 1100
AjnI CCWGG 1 cut(s) 1081
AluBI AGCT 9 cut(s) 265, 331, 346, 375, 391, 401, 869, 887, 1065
AluI AGCT 9 cut(s) 265, 331, 346, 375, 391, 401, 869, 887, 1065
AlwI GGATC 3 cut(s) 104, 685, 1073
AoxI GGCC 2 cut(s) 543, 717
ApaI GGGCCC 1 cut(s) 547
ApeKI GCWGC 6 cut(s) 80, 148, 259, 262, 485, 773
ApoI RAATTY 3 cut(s) 52, 937, 1008
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspLEI GCGC 2 cut(s) 148, 916
AspS9I GGNCC 4 cut(s) 137, 543, 544, 849
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 137, 849
AxyI CCTNAGG 1 cut(s) 218
BaeGI GKGCMC 2 cut(s) 547, 769
BanII GRGCYC 2 cut(s) 48, 547
BbvI GCAGC 6 cut(s) 92, 135, 271, 274, 472, 760
BccI CCATC 3 cut(s) 537, 707, 821
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 3 cut(s) 33, 932, 966
BciT130I CCWGG 1 cut(s) 1083
BclI TGATCA 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 1008
BisI GCNGC 8 cut(s) 81, 149, 238, 260, 263, 286, 486, 774
BlpI GCTNAGC 1 cut(s) 371
BlsI GCNGC 8 cut(s) 82, 150, 239, 261, 264, 287, 487, 775
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 1083
Bme18I GGWCC 2 cut(s) 137, 849
BmgT120I GGNCC 4 cut(s) 137, 543, 544, 849
BmiI GGNNCC 4 cut(s) 241, 544, 545, 998
BmrFI CCNGG 1 cut(s) 1083
BmsI GCATC 2 cut(s) 357, 750
Bpu1102I GCTNAGC 1 cut(s) 371
BpuEI CTTGAG 4 cut(s) 263, 308, 347, 1051
Bsa29I ATCGAT 2 cut(s) 952, 1077
BsaAI YACGTR 1 cut(s) 855
BsaJI CCNNGG 3 cut(s) 92, 243, 472
BsaXI ACNNNNNCTCC 4 cut(s) 197, 224, 227, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 846, 1088
Bse1I ACTGG 3 cut(s) 679, 774, 840
Bse21I CCTNAGG 1 cut(s) 218
BseBI CCWGG 1 cut(s) 1083
BseCI ATCGAT 2 cut(s) 952, 1077
BseDI CCNNGG 3 cut(s) 92, 243, 472
BseGI GGATG 2 cut(s) 765, 832
BseLI CCNNNNNNNGG 2 cut(s) 846, 1088
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 3 cut(s) 679, 774, 840
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 2 cut(s) 547, 769
BseXI GCAGC 6 cut(s) 92, 135, 271, 274, 472, 760
BseYI CCCAGC 1 cut(s) 694
Bsh1236I CGCG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 545, 719
BshVI ATCGAT 2 cut(s) 952, 1077
BsiEI CGRYCG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 683
BslI CCNNNNNNNGG 2 cut(s) 846, 1088
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 2 cut(s) 545, 719
Bsp120I GGGCCC 1 cut(s) 543
Bsp1286I GDGCHC 3 cut(s) 48, 547, 769
Bsp143I GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
Bsp1720I GCTNAGC 1 cut(s) 371
Bsp68I TCGCGA 1 cut(s) 336
BspACI CCGC 6 cut(s) 163, 184, 237, 286, 488, 763
BspANI GGCC 2 cut(s) 545, 719
BspCNI CTCAG 1 cut(s) 210
BspDI ATCGAT 2 cut(s) 952, 1077
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 4 cut(s) 241, 544, 545, 998
BspMI ACCTGC 1 cut(s) 1008
BspPI GGATC 3 cut(s) 104, 685, 1073
BspQI GCTCTTC 1 cut(s) 164
BsrI ACTGG 3 cut(s) 679, 774, 840
BssECI CCNNGG 3 cut(s) 92, 243, 472
BssMI GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
BssT1I CCWWGG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 4 cut(s) 156, 430, 557, 563
Bst6I CTCTTC 4 cut(s) 110, 164, 406, 815
BstAPI GCANNNNNTGC 2 cut(s) 773, 911
BstBAI YACGTR 1 cut(s) 855
BstC8I GCNNGC 5 cut(s) 290, 344, 765, 889, 1038
BstDEI CTNAG 2 cut(s) 218, 371
BstF5I GGATG 2 cut(s) 765, 832
BstFNI CGCG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 2 cut(s) 148, 916
BstKTI GATC 8 cut(s) 99, 439, 457, 508, 693, 952, 956, 1081
BstMBI GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 2 cut(s) 773, 911
BstNI CCWGG 1 cut(s) 1083
BstNSI RCATGY 2 cut(s) 41, 895
BstSCI CCNGG 1 cut(s) 1081
BstSLI GKGCMC 2 cut(s) 547, 769
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 6 cut(s) 92, 135, 271, 274, 472, 760
BstX2I RGATCY 1 cut(s) 690
BstYI RGATCY 1 cut(s) 690
Bsu15I ATCGAT 2 cut(s) 952, 1077
Bsu36I CCTNAGG 1 cut(s) 218
BsuRI GGCC 2 cut(s) 545, 719
BsuTUI ATCGAT 2 cut(s) 952, 1077
BtsCI GGATG 2 cut(s) 765, 832
BtsIMutI CAGTG 3 cut(s) 439, 767, 847
BtuMI TCGCGA 1 cut(s) 336
BveI ACCTGC 1 cut(s) 1008
Cac8I GCNNGC 5 cut(s) 290, 344, 765, 889, 1038
CfoI GCGC 2 cut(s) 148, 916
Cfr13I GGNCC 4 cut(s) 137, 543, 544, 849
ClaI ATCGAT 2 cut(s) 952, 1077
Csp6I GTAC 2 cut(s) 196, 214
CviAII CATG 4 cut(s) 38, 637, 730, 892
CviQI GTAC 2 cut(s) 196, 214
DdeI CTNAG 2 cut(s) 218, 371
DpnI GATC 8 cut(s) 98, 438, 456, 507, 692, 951, 955, 1080
DpnII GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
Eam1104I CTCTTC 4 cut(s) 110, 164, 406, 815
EarI CTCTTC 4 cut(s) 110, 164, 406, 815
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 719
Eco24I GRGCYC 2 cut(s) 48, 547
Eco47I GGWCC 2 cut(s) 137, 849
Eco81I CCTNAGG 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 543, 544
EcoRII CCWGG 1 cut(s) 1081
EcoT14I CCWWGG 1 cut(s) 92
EcoT38I GRGCYC 2 cut(s) 48, 547
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 4 cut(s) 41, 640, 733, 895
FatI CATG 4 cut(s) 37, 636, 729, 891
FauI CCCGC 1 cut(s) 756
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 8 cut(s) 81, 149, 238, 260, 263, 286, 486, 774
FokI GGATG 2 cut(s) 772, 839
FriOI GRGCYC 2 cut(s) 48, 547
Fsp4HI GCNGC 8 cut(s) 81, 149, 238, 260, 263, 286, 486, 774
GlaI GCGC 2 cut(s) 147, 915
GluI GCNGC 8 cut(s) 81, 149, 238, 260, 263, 286, 486, 774
GsaI CCCAGC 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 545, 719
HapII CCGG 1 cut(s) 683
HhaI GCGC 2 cut(s) 148, 916
Hin1II CATG 4 cut(s) 41, 640, 733, 895
Hin6I GCGC 2 cut(s) 146, 914
HinP1I GCGC 2 cut(s) 146, 914
HincII GTYRAC 1 cut(s) 1094
HindII GTYRAC 1 cut(s) 1094
HindIII AAGCTT 2 cut(s) 329, 344
HinfI GANTC 2 cut(s) 515, 737
HpaII CCGG 1 cut(s) 683
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 298, 1094
Hpy188I TCNGA 5 cut(s) 134, 244, 397, 473, 1071
Hpy188III TCNNGA 7 cut(s) 280, 325, 335, 364, 452, 512, 757
Hpy8I GTNNAC 2 cut(s) 298, 1094
HpyAV CCTTC 3 cut(s) 604, 709, 895
HpyCH4III ACNGT 4 cut(s) 156, 430, 557, 563
HpyCH4IV ACGT 1 cut(s) 854
HpyCH4V TGCA 4 cut(s) 80, 151, 943, 1055
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 911
HpyF3I CTNAG 2 cut(s) 218, 371
HpySE526I ACGT 1 cut(s) 854
Hsp92II CATG 4 cut(s) 41, 640, 733, 895
HspAI GCGC 2 cut(s) 146, 914
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 1 cut(s) 245
Lsp1109I GCAGC 6 cut(s) 92, 135, 271, 274, 472, 760
LweI GCATC 2 cut(s) 357, 750
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 3 cut(s) 175, 536, 557
MalI GATC 8 cut(s) 98, 438, 456, 507, 692, 951, 955, 1080
MboI GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
MboII GAAGA 6 cut(s) 97, 181, 423, 500, 832, 943
MflI RGATCY 1 cut(s) 690
MhlI GDGCHC 3 cut(s) 48, 547, 769
MluCI AATT 8 cut(s) 32, 52, 127, 569, 873, 923, 937, 1008
MseI TTAA 5 cut(s) 380, 618, 629, 780, 918
MspI CCGG 1 cut(s) 683
MspR9I CCNGG 1 cut(s) 1083
Mva1269I GAATGC 1 cut(s) 477
MvaI CCWGG 1 cut(s) 1083
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 773, 911
NdeII GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
NlaIII CATG 4 cut(s) 41, 640, 733, 895
NlaIV GGNNCC 4 cut(s) 241, 544, 545, 998
NmuCI GTSAC 1 cut(s) 175
NruI TCGCGA 1 cut(s) 336
NspI RCATGY 2 cut(s) 41, 895
PceI AGGCCT 1 cut(s) 719
PciI ACATGT 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 3 cut(s) 185, 240, 1021
PctI GAATGC 1 cut(s) 477
PfeI GAWTC 2 cut(s) 515, 737
PflMI CCANNNNNTGG 2 cut(s) 846, 1088
PkrI GCNGC 8 cut(s) 82, 150, 239, 261, 264, 287, 487, 775
Ppu21I YACGTR 1 cut(s) 855
PscI ACATGT 1 cut(s) 37
PsiI TTATAA 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 1081
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 4 cut(s) 241, 544, 545, 998
PspOMI GGGCCC 1 cut(s) 543
PspPI GGNCC 4 cut(s) 137, 543, 544, 849
PsuI RGATCY 1 cut(s) 690
RruI TCGCGA 1 cut(s) 336
RsaI GTAC 2 cut(s) 197, 215
RsaNI GTAC 2 cut(s) 196, 214
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 5 cut(s) 380, 618, 629, 780, 918
SatI GCNGC 8 cut(s) 81, 149, 238, 260, 263, 286, 486, 774
Sau3AI GATC 8 cut(s) 96, 436, 454, 505, 690, 949, 953, 1078
Sau96I GGNCC 4 cut(s) 137, 543, 544, 849
ScaI AGTACT 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 1083
SduI GDGCHC 3 cut(s) 48, 547, 769
SfaNI GCATC 2 cut(s) 357, 750
SinI GGWCC 2 cut(s) 137, 849
SmlI CTYRAG 4 cut(s) 278, 323, 362, 1030
SmoI CTYRAG 4 cut(s) 278, 323, 362, 1030
Sse9I AATT 8 cut(s) 32, 52, 127, 569, 873, 923, 937, 1008
SseBI AGGCCT 1 cut(s) 719
SsiI CCGC 6 cut(s) 163, 184, 237, 286, 488, 763
StuI AGGCCT 1 cut(s) 719
StyD4I CCNGG 1 cut(s) 1081
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 4 cut(s) 156, 430, 557, 563
TaiI ACGT 1 cut(s) 857
TaqI TCGA 6 cut(s) 67, 188, 735, 832, 952, 1077
TasI AATT 8 cut(s) 32, 52, 127, 569, 873, 923, 937, 1008
TatI WGTACW 1 cut(s) 195
TauI GCSGC 2 cut(s) 240, 288
TfiI GAWTC 2 cut(s) 515, 737
Tru1I TTAA 5 cut(s) 380, 618, 629, 780, 918
Tru9I TTAA 5 cut(s) 380, 618, 629, 780, 918
TscAI CASTG 3 cut(s) 439, 774, 847
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 6 cut(s) 80, 148, 259, 262, 485, 773
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 8 cut(s) 63, 285, 434, 490, 623, 728, 735, 833
TspRI CASTG 3 cut(s) 439, 774, 847
Van91I CCANNNNNTGG 2 cut(s) 846, 1088
VpaK11BI GGWCC 2 cut(s) 137, 849
XapI RAATTY 3 cut(s) 52, 937, 1008
XceI RCATGY 2 cut(s) 41, 895
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.