pycom16g08440

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010144
Physical Location & Seq
Forward (+)
5604546 .. 5607007
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGACTTACAGTCCGAAGCGTGCCATGGCGGAGCTCAGGCACTCCATCGCGGTGGGGGCTCGAGCCACTTCTTCTCCAATGAAGCGCGACGAGGACTCCTCCCCTCTGATCTTCGACACTCAGCCCCAAGACGACGACGACGGCCGCGGTCGCCCTCCCCACAAAGATCGCGACCGTCCCTTCCTCTCCCATTTCCAATCCATCTGCCCCTCAATTGCCGACGACTCCAACAGCTCCAGGATCTCACTCTTCATCGTCATCGTCGTCGTCGCAGTCTTCGGCCTGGTCTCGATTCTGGCGATCGTGAAACGAGTGAATACTCCTTACTTGTGTAAAAAAGATGGCATTACTCTTCACTGTCCTCTTGTCAAGGAGTCGCCTTCGCTTTGGGAGAATCCGTATTCAGCAACCACGTCTTGGAAGCCCTGTGCCGAGCGCCGCCTTGGTGGGATTTCAGATCTTCCACCTGAAAACGAAACAACTGGGTACATATTTATTCATGCTGAGGGTGGCCTAAATCAGCAAAGAATTGCGATATGCAATGCCGTAGCTGTAGCCAAAATAATGAATGCCACCCTTATTTTGCCTGTGCTGAAGCAGGACCAGATTTGGAAAGACCAAACGAAATTTGAAGATATCTTTGATGTTGACCATTTCATTGATTACCTGAAGGATGATGTACGAATTGTTCGTGATATTCCTGAGTGGTTTACTGACAAAGCTGAGCTATTTACAAGTATAAGACGAACAGTAAAGAACATTCCAAAATATGCACCTGCTCAATTTTACATAGACAATGTTCTGCCGCGAATCAAGGAGAAGAAGATAATGGCCCTAAAACCTTTTGTCGATCGATCGGGTACGACAATGTCCCTCAAGAAATCAACAGGCTAA

Protein Analysis

298

Amino Acids

33.52

Weight (kDa)

8.69

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 163 - 288 7.9e-36 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26370 AT3G26370
fragaria_vesca FvH4_4g25921
malus_domestica MD13G1098200.v1.1 MD16G1099100.v1.1
prunus_persica Prupe.1G247100_v2.0.a1
pyrus_communis pycom13g08580 pycom13g08590 pycom16g08440 pycom16g08450
rosa_chinensis RchiOBHm_Chr4g0433391
rosa_laevigata RLG00000006757
rosa_multiflora Rmu_sc0000759.1_g000007
rosa_roxburghii Rroxscaffold_5G00374940
rosa_rugosa Rorug04G0268400
rosa_samantha Rh4AG323200 Rh4BG330800 Rh4BG330900 Rh4CG346200 Rh4DG327300
rosa_wichuraiana Rw4G027990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
Acc36I ACCTGC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 417
AccII CGCG 5 cut(s) 50, 87, 147, 171, 808
AciI CCGC 6 cut(s) 29, 50, 145, 147, 439, 806
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 2 cut(s) 614, 689
AfaI GTAC 3 cut(s) 488, 681, 862
AfiI CCNNNNNNNGG 1 cut(s) 417
AgsI TTSAA 1 cut(s) 632
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 2 cut(s) 236, 282
AluBI AGCT 5 cut(s) 34, 234, 551, 722, 727
AluI AGCT 5 cut(s) 34, 234, 551, 722, 727
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 60
AoxI GGCC 4 cut(s) 142, 280, 511, 831
ApoI RAATTY 1 cut(s) 626
AspLEI GCGC 2 cut(s) 87, 438
AspS9I GGNCC 2 cut(s) 601, 832
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 1 cut(s) 601
BanII GRGCYC 2 cut(s) 36, 61
BbsI GAAGAC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 36
BbvCI CCTCAGC 1 cut(s) 504
BccI CCATC 3 cut(s) 53, 209, 335
BceAI ACGGC 2 cut(s) 157, 530
BciT130I CCWGG 2 cut(s) 238, 284
BcoDI GTCTC 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 784
BglII AGATCT 1 cut(s) 457
BisI GCNGC 3 cut(s) 145, 439, 806
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 3 cut(s) 146, 440, 807
Bme1390I CCNGG 2 cut(s) 238, 284
Bme18I GGWCC 1 cut(s) 601
BmeRI GACNNNNNGTC 1 cut(s) 9
BmeT110I CYCGRG 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 2 cut(s) 601, 832
BmrFI CCNGG 2 cut(s) 238, 284
BmrI ACTGGG 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 268
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 220
Bpu10I CCTNAGC 2 cut(s) 35, 504
Bpu1102I GCTNAGC 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 860
Bsa29I ATCGAT 1 cut(s) 853
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 24, 145, 442
BsaXI ACNNNNNCTCC 6 cut(s) 58, 80, 88, 110, 383, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 1 cut(s) 547
BseBI CCWGG 2 cut(s) 238, 284
BseCI ATCGAT 1 cut(s) 853
BseDI CCNNGG 3 cut(s) 24, 145, 442
BseGI GGATG 1 cut(s) 679
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 5 cut(s) 49, 134, 495, 693, 714
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 88
BseX3I CGGCCG 1 cut(s) 142
Bsh1236I CGCG 5 cut(s) 50, 87, 147, 171, 808
Bsh1285I CGRYCG 6 cut(s) 145, 151, 175, 303, 853, 857
BshFI GGCC 4 cut(s) 144, 282, 513, 833
BshVI ATCGAT 1 cut(s) 853
BsiEI CGRYCG 6 cut(s) 145, 151, 175, 303, 853, 857
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 60
BslFI GGGAC 2 cut(s) 162, 856
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 1 cut(s) 292
BsmFI GGGAC 2 cut(s) 162, 856
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 4 cut(s) 144, 282, 513, 833
Bso31I GGTCTC 1 cut(s) 292
BsoBI CYCGRG 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 36, 61
Bsp143I GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
Bsp1720I GCTNAGC 1 cut(s) 723
Bsp19I CCATGG 1 cut(s) 24
Bsp68I TCGCGA 1 cut(s) 171
BspACI CCGC 6 cut(s) 29, 50, 145, 147, 439, 806
BspANI GGCC 4 cut(s) 144, 282, 513, 833
BspCNI CTCAG 5 cut(s) 48, 133, 496, 694, 715
BspDI ATCGAT 1 cut(s) 853
BspFNI CGCG 5 cut(s) 50, 87, 147, 171, 808
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 547
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 3 cut(s) 24, 145, 442
BssMI GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
BssT1I CCWWGG 2 cut(s) 24, 442
Bst2UI CCWGG 2 cut(s) 238, 284
Bst4CI ACNGT 4 cut(s) 11, 176, 359, 751
Bst6I CTCTTC 2 cut(s) 254, 357
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 5 cut(s) 35, 120, 504, 702, 723
BstDSI CCRYGG 2 cut(s) 24, 145
BstF5I GGATG 1 cut(s) 679
BstFNI CGCG 5 cut(s) 50, 87, 147, 171, 808
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 87, 438
BstKTI GATC 7 cut(s) 111, 169, 243, 303, 460, 853, 857
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
BstMCI CGRYCG 6 cut(s) 145, 151, 175, 303, 853, 857
BstMWI GCNNNNNNNGC 2 cut(s) 56, 150
BstNI CCWGG 2 cut(s) 238, 284
BstSCI CCNGG 2 cut(s) 236, 282
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 5 cut(s) 50, 87, 147, 171, 808
BstV2I GAAGAC 1 cut(s) 268
BstX2I RGATCY 2 cut(s) 240, 457
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 2 cut(s) 240, 457
BstZI CGGCCG 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 853
BsuRI GGCC 4 cut(s) 144, 282, 513, 833
BsuTUI ATCGAT 1 cut(s) 853
BtgI CCRYGG 2 cut(s) 24, 145
BtgZI GCGATG 1 cut(s) 31
BtrI CACGTC 1 cut(s) 414
BtsCI GGATG 1 cut(s) 679
BtsIMutI CAGTG 1 cut(s) 355
BtuMI TCGCGA 1 cut(s) 171
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 2 cut(s) 87, 438
Cfr13I GGNCC 2 cut(s) 601, 832
Cfr42I CCGCGG 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 853
Csp6I GTAC 3 cut(s) 487, 680, 861
CviAII CATG 2 cut(s) 25, 500
CviQI GTAC 3 cut(s) 487, 680, 861
DdeI CTNAG 5 cut(s) 35, 120, 504, 702, 723
DpnI GATC 7 cut(s) 110, 168, 242, 302, 459, 852, 856
DpnII GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
DriI GACNNNNNGTC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 142
EagI CGGCCG 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 254, 357
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 2 cut(s) 254, 357
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 24, 442
Eco24I GRGCYC 2 cut(s) 36, 61
Eco31I GGTCTC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 601
Eco52I CGGCCG 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 614, 689
Eco88I CYCGRG 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 236, 282
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 24, 442
EcoT38I GRGCYC 2 cut(s) 36, 61
ErhI CCWWGG 2 cut(s) 24, 442
FaeI CATG 2 cut(s) 28, 503
FaiI YATR 7 cut(s) 26, 491, 501, 538, 740, 771, 791
FaqI GGGAC 2 cut(s) 162, 856
FatI CATG 2 cut(s) 24, 499
Fnu4HI GCNGC 3 cut(s) 145, 439, 806
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 2 cut(s) 36, 61
Fsp4HI GCNGC 3 cut(s) 145, 439, 806
GlaI GCGC 2 cut(s) 86, 437
GluI GCNGC 3 cut(s) 145, 439, 806
GsuI CTGGAG 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 4 cut(s) 144, 282, 513, 833
HhaI GCGC 2 cut(s) 87, 438
Hin1II CATG 2 cut(s) 28, 503
Hin6I GCGC 2 cut(s) 85, 436
HinP1I GCGC 2 cut(s) 85, 436
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 6 cut(s) 95, 224, 292, 374, 394, 810
Hpy166II GTNNAC 2 cut(s) 649, 711
Hpy188I TCNGA 3 cut(s) 15, 108, 457
Hpy188III TCNNGA 6 cut(s) 170, 289, 304, 692, 701, 877
Hpy8I GTNNAC 2 cut(s) 649, 711
Hpy99I CGWCG 8 cut(s) 92, 137, 140, 143, 224, 266, 269, 272
HpyAV CCTTC 3 cut(s) 190, 390, 664
HpyCH4III ACNGT 4 cut(s) 11, 176, 359, 751
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 2 cut(s) 540, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 150
HpyF3I CTNAG 5 cut(s) 35, 120, 504, 702, 723
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 2 cut(s) 28, 503
HspAI GCGC 2 cut(s) 85, 436
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
LmnI GCTCC 2 cut(s) 31, 239
MaeII ACGT 1 cut(s) 413
MalI GATC 7 cut(s) 110, 168, 242, 302, 459, 852, 856
MboI GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
MboII GAAGA 9 cut(s) 63, 103, 241, 268, 344, 452, 644, 832, 835
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 240, 457
MhlI GDGCHC 2 cut(s) 36, 61
MluCI AATT 5 cut(s) 213, 528, 626, 684, 782
MlyI GAGTC 3 cut(s) 89, 218, 383
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 9 cut(s) 85, 109, 114, 165, 194, 220, 372, 499, 884
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 238, 284
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 2 cut(s) 238, 284
MvnI CGCG 5 cut(s) 50, 87, 147, 171, 808
MwoI GCNNNNNNNGC 2 cut(s) 56, 150
NcoI CCATGG 1 cut(s) 24
NdeII GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
NlaIII CATG 2 cut(s) 28, 503
NmeAIII GCCGAG 1 cut(s) 457
NruI TCGCGA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 784
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 296, 688
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 292, 394, 810
PflFI GACNNNGTC 1 cut(s) 868
PflMI CCANNNNNTGG 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 236
PkrI GCNGC 3 cut(s) 146, 440, 807
Ple19I CGATCG 3 cut(s) 303, 853, 857
PleI GAGTC 3 cut(s) 89, 218, 382
PpsI GAGTC 3 cut(s) 89, 218, 382
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 236, 282
PspGI CCWGG 2 cut(s) 236, 282
PspPI GGNCC 2 cut(s) 601, 832
PspXI VCTCGAGB 1 cut(s) 60
PsrI GAACNNNNNNTAC 2 cut(s) 672, 704
PsuI RGATCY 2 cut(s) 240, 457
PsyI GACNNNGTC 1 cut(s) 868
PvuI CGATCG 3 cut(s) 303, 853, 857
RruI TCGCGA 1 cut(s) 171
RsaI GTAC 3 cut(s) 488, 681, 862
RsaNI GTAC 3 cut(s) 487, 680, 861
RseI CAYNNNNRTG 1 cut(s) 50
SacI GAGCTC 1 cut(s) 36
SacII CCGCGG 1 cut(s) 148
SatI GCNGC 3 cut(s) 145, 439, 806
Sau3AI GATC 7 cut(s) 108, 166, 240, 300, 457, 850, 854
Sau96I GGNCC 2 cut(s) 601, 832
SchI GAGTC 3 cut(s) 89, 218, 383
ScrFI CCNGG 2 cut(s) 238, 284
SduI GDGCHC 2 cut(s) 36, 61
SfcI CTRYAG 1 cut(s) 552
Sfr274I CTCGAG 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 148
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 1 cut(s) 601
SlaI CTCGAG 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 2 cut(s) 60, 875
SmoI CTYRAG 2 cut(s) 60, 875
Sse9I AATT 5 cut(s) 213, 528, 626, 684, 782
SsiI CCGC 6 cut(s) 29, 50, 145, 147, 439, 806
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 236, 282
StyI CCWWGG 2 cut(s) 24, 442
TaaI ACNGT 4 cut(s) 11, 176, 359, 751
TaiI ACGT 1 cut(s) 416
TaqI TCGA 5 cut(s) 61, 114, 290, 849, 853
TasI AATT 5 cut(s) 213, 528, 626, 684, 782
TauI GCSGC 3 cut(s) 147, 441, 808
TfiI GAWTC 3 cut(s) 292, 394, 810
TscAI CASTG 1 cut(s) 362
TspDTI ATGAA 5 cut(s) 95, 241, 488, 581, 646
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 1 cut(s) 362
Tth111I GACNNNGTC 1 cut(s) 868
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 1 cut(s) 601
XapI RAATTY 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.