Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0014248

Physical Location & Seq

Genomic Coordinates Forward (+)

5626676 .. 5629851

Gene Structure 3176 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGACAAACCCTGGGGTACTGTCGGCAACTGGGCCGCCGAGGCTGAGCGAGAAGAGGCCGAGGAGCTCGCGGCCGCCAAGGTTGAGTCCCAGAGCTTCCCCAGCCTAAAGGAAGCCGTCAGCGCCAAGACCAAGAAGAAGAAGATGAGCCTCTCTGAATTCTACAACACCTCCGCGACCCCCGCCTCTAATCGTGTCGGCCTCACTCCCGACGAGATGATGCGCCTCCCTACAGGCCCCAAGGAGCGCTCTGCCGAAGAAATGCAGTACGGTCGCCTTGGTGGCGGGTTTCCCTCCTACGGTCGCTCTGGTACGAATCCGGGTCGGGGCGGGGACAGAGAGGGCGGGGACGGGTCGTGGGGTGGTGGTGGAAGAAGATCCTACGGTGGATTCGACGACGACAGGAGGGGCGGCCCGCCTTCTAGGGTTTCTGATTTAGATCAACCGTCAAGGGCGGACGAGGTTGACAATTGGGCAATGACTAAAAAGTCTCTTCCTTCTGTAGATTCCGGTAGGCAGAACCGGTATAGCTCGCTCGGTAGTGTTGGTGCTGGGGGTGGGGGTGGTGGTGGTGTTGGTGGTGGTATGGGCGGCCCAGCTTGGTCTAGGGCAGACGAGGTTGATAACTGGGCTGTTGGGAAGAAGCCTGCTCCTTCTAGGTCATCCCCATTTGGGTCTGAATTTCATTCGGGTCCGGAACCGGATCGCTGGGCGAGAGGCGGACGCGAGGTCGACAGAGTTGAGAGAGAGCGACCCAAATTGGTTTTGGATCCTCCGAAAGTCGAGGCCGGCGTGAATGAGCCATCGCAGGTTGTGAAGACCAATAAGCCGAACCCCTTCGGAGCAGCTCGACCTAGGGAGGAGATTTTGGCTGGGAAAGGGTTGGATTGGAAGAAGCTGGAGTCGGAAATTGAAGCCAAGAAGACGAGTAGGCCGACGAGTGCCCACTCAAGCAGGCCTTCGAGTGCTCAGTCAAGCCGGTCTGAAGGGCCCGGGCTGCATGCAAGTGATAATGTGGTGAAGCCGCGGCCGAAAGTAAACCCCTTTGGTGATGCCAAGCCAAGGGAGGTTTTGCTGGAGGAGCGGGGTAAGGATTGGCGGAAGATTGATCTCGAGCTGGAGCATCAGGGTATTGACAGACCTGAAACAGAGGAGGAAAAGAGATTGAAGGAGGAAATAGATCATCTAAAGAAGGAACTTGAGAAAGAATCTACGGATAAAGTGAACAGTGAAACGGTGCAAGAGTCTGGTGGTGATCAGCCAAGTCTACGTGATATAGTACTACAGAAGGAAAGGGAATTGGAGGCATTGATACGAGAATTTGATGACAAAGTTCGCTTTGGGCAGAAGGCCATCGATAGGCCAGGGTCTGGGGCTGGTAGGCCTGGGTCTGGGGCAGGAAGGCCTGGGTCTTCAGCAGGGAGGGCCGGTAACTTTCATGAGAGGACGCCGTCTTATTCGGGGTCATTTGAAGATTCTAGAAGCGTGGAATACGTAGATAGGTCTCCCTTGCAAGGCAAAGGAGATCCGTGGGCTAGGCCTTTTGATGACAGAAGGTCATTCCATGGTGGCAGGGAAAGAGGAGGATTTCTGGGGAGCAGAGACATGGGCAGGGCAAGGTCAAGAGAAAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009505 GO:0009506 GO:0009987 GO:0010467 GO:0019538 GO:0030054 GO:0030312 GO:0034641 GO:0034645 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044464 GO:0046983 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-4B

Protein Analysis

546

Amino Acids

59.33

Weight (kDa)

8.68

Isoelectric Point (pI)

52.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-4B	PF06273	1 - 464	2.2e-208	Plant specific eukaryotic initiation factor 4B
eIF-4B	PF06273	467 - 528	2.7e-17	Plant specific eukaryotic initiation factor 4B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13020
fragaria_vesca	FvH4_4g26000
malus_domestica	MD13G1098600.v1.1 MD16G1099600.v1.1
prunus_persica	Prupe.1G246800_v2.0.a1 Prupe.1G246800_v2.0.a1
pyrus_communis	pycom13g08630 pycom16g08470
rosa_chinensis	RchiOBHm_Chr4g0433421
rosa_laevigata	RLG00000006754
rosa_roxburghii	Rroxscaffold_5G00374970
rosa_rugosa	Rorug04G0268700
rosa_samantha	Rh4AG323400 Rh4BG331300 Rh4CG346600 Rh4DG327600
rosa_wichuraiana	Rw4G028020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	487
Acc36I	ACCTGC	1 cut(s)	799
AccB7I	CCANNNNNTGG	1 cut(s)	1370
AccBSI	CCGCTC	1 cut(s)	1084
AccI	GTMKAC	2 cut(s)	732, 1267
AccII	CGCG	4 cut(s)	71, 176, 726, 1027
AccIII	TCCGGA	1 cut(s)	694
AclWI	GGATC	5 cut(s)	372, 711, 764, 777, 1520
AcoI	YGGCCR	2 cut(s)	72, 1028
AcsI	RAATTY	3 cut(s)	158, 680, 1319
AcuI	CTGAAG	2 cut(s)	1005, 1398
AcyI	GRCGYC	1 cut(s)	1448
AfaI	GTAC	4 cut(s)	19, 269, 313, 1281
AfeI	AGCGCT	1 cut(s)	248
AfiI	CCNNNNNNNGG	8 cut(s)	299, 326, 672, 1062, 1359, 1370, 1391, 1514
AgeI	ACCGGT	1 cut(s)	522
AgsI	TTSAA	3 cut(s)	914, 1168, 1472
AhdI	GACNNNNNGTC	2 cut(s)	728, 1555
AjnI	CCWGG	4 cut(s)	11, 1363, 1384, 1405
AluBI	AGCT	7 cut(s)	67, 96, 531, 599, 848, 898, 1117
AluI	AGCT	7 cut(s)	67, 96, 531, 599, 848, 898, 1117
Alw21I	GWGCWC	2 cut(s)	69, 970
Alw26I	GTCTC	3 cut(s)	495, 1509, 1596
AlwI	GGATC	5 cut(s)	372, 711, 764, 777, 1520
AlwNI	CAGNNNCTG	1 cut(s)	1370
Ama87I	CYCGRG	2 cut(s)	992, 1112
Aor13HI	TCCGGA	1 cut(s)	694
Aor51HI	AGCGCT	1 cut(s)	248
ApaI	GGGCCC	1 cut(s)	993
ApeKI	GCWGC	2 cut(s)	845, 997
ApoI	RAATTY	3 cut(s)	158, 680, 1319
AsiGI	ACCGGT	1 cut(s)	522
Asp700I	GAANNNNTTC	1 cut(s)	836
AspA2I	CCTAGG	1 cut(s)	854
AspLEI	GCGC	3 cut(s)	125, 225, 249
AspS9I	GGNCC	8 cut(s)	33, 236, 413, 593, 692, 989, 990, 1425
AsuC2I	CCSGG	3 cut(s)	321, 993, 994
AsuHPI	GGTGA	3 cut(s)	1030, 1061, 1265
AvaI	CYCGRG	2 cut(s)	992, 1112
AvaII	GGWCC	1 cut(s)	692
AvrII	CCTAGG	1 cut(s)	854
BaeGI	GKGCMC	2 cut(s)	946, 993
BamHI	GGATCC	1 cut(s)	769
BanII	GRGCYC	2 cut(s)	69, 993
BbsI	GAAGAC	3 cut(s)	824, 929, 1404
Bbv12I	GWGCWC	2 cut(s)	69, 970
BbvI	GCAGC	2 cut(s)	857, 984
BccI	CCATC	3 cut(s)	811, 1361, 1626
BceAI	ACGGC	2 cut(s)	101, 1435
BcgI	CGANNNNNNTGC	2 cut(s)	254, 288
BciT130I	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BclI	TGATCA	1 cut(s)	1255
BcnI	CCSGG	3 cut(s)	321, 993, 994
BcoDI	GTCTC	3 cut(s)	495, 1509, 1596
BfaI	CTAG	6 cut(s)	423, 606, 657, 855, 1479, 1536
BfmI	CTRYAG	3 cut(s)	231, 501, 1283
BfoI	RGCGCY	2 cut(s)	126, 250
BfuAI	ACCTGC	1 cut(s)	799
BglI	GCCNNNNNGGC	2 cut(s)	41, 282
BisI	GCNGC	9 cut(s)	36, 72, 75, 412, 592, 846, 998, 1025, 1028
BlnI	CCTAGG	1 cut(s)	854
BlpI	GCTNAGC	1 cut(s)	45
BlsI	GCNGC	9 cut(s)	37, 73, 76, 413, 593, 847, 999, 1026, 1029
BmcAI	AGTACT	1 cut(s)	1281
Bme1390I	CCNGG	7 cut(s)	13, 321, 993, 994, 1365, 1386, 1407
Bme18I	GGWCC	1 cut(s)	692
BmeRI	GACNNNNNGTC	2 cut(s)	728, 1555
BmeT110I	CYCGRG	2 cut(s)	992, 1112
BmgT120I	GGNCC	8 cut(s)	33, 236, 413, 593, 692, 989, 990, 1425
BmiI	GGNNCC	5 cut(s)	238, 693, 699, 771, 991
BmrFI	CCNGG	7 cut(s)	13, 321, 993, 994, 1365, 1386, 1407
BmrI	ACTGGG	2 cut(s)	40, 637
BmsI	GCATC	3 cut(s)	210, 1042, 1132
BmuI	ACTGGG	2 cut(s)	40, 637
BpiI	GAAGAC	3 cut(s)	824, 929, 1404
BpmI	CTGGAG	3 cut(s)	920, 1097, 1139
Bpu1102I	GCTNAGC	1 cut(s)	45
BpuEI	CTTGAG	2 cut(s)	934, 1220
BpuMI	CCSGG	3 cut(s)	321, 993, 994
Bsa29I	ATCGAT	1 cut(s)	1356
BsaAI	YACGTR	2 cut(s)	1271, 1495
BsaBI	GATNNNNATC	1 cut(s)	438
BsaHI	GRCGYC	1 cut(s)	1448
BsaI	GGTCTC	1 cut(s)	1509
BsaWI	WCCGGW	4 cut(s)	509, 522, 694, 700
BsaXI	ACNNNNNCTCC	4 cut(s)	155, 185, 1414, 1444
Bsc4I	CCNNNNNNNGG	8 cut(s)	299, 326, 672, 1062, 1359, 1370, 1391, 1514
Bse118I	RCCGGY	4 cut(s)	522, 788, 978, 1427
Bse1I	ACTGG	2 cut(s)	35, 632
Bse3DI	GCAATG	1 cut(s)	483
Bse8I	GATNNNNATC	1 cut(s)	438
BseAI	TCCGGA	1 cut(s)	694
BseBI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BseCI	ATCGAT	1 cut(s)	1356
BseGI	GGATG	1 cut(s)	662
BseJI	GATNNNNATC	1 cut(s)	438
BseLI	CCNNNNNNNGG	8 cut(s)	299, 326, 672, 1062, 1359, 1370, 1391, 1514
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	2 cut(s)	36, 983
BseNI	ACTGG	2 cut(s)	35, 632
BseRI	GAGGAG	5 cut(s)	77, 875, 1094, 1166, 1596
BseSI	GKGCMC	2 cut(s)	946, 993
BseX3I	CGGCCG	2 cut(s)	72, 1028
BseXI	GCAGC	2 cut(s)	857, 984
BseYI	CCCAGC	5 cut(s)	101, 551, 595, 708, 872
Bsh1236I	CGCG	4 cut(s)	71, 176, 726, 1027
Bsh1285I	CGRYCG	4 cut(s)	75, 274, 304, 1031
BshTI	ACCGGT	1 cut(s)	522
BshVI	ATCGAT	1 cut(s)	1356
BsiEI	CGRYCG	4 cut(s)	75, 274, 304, 1031
BsiHKAI	GWGCWC	2 cut(s)	69, 970
BsiHKCI	CYCGRG	2 cut(s)	992, 1112
BsiSI	CCGG	9 cut(s)	320, 510, 523, 695, 701, 789, 979, 993, 1428
BslFI	GGGAC	3 cut(s)	73, 347, 362
BslI	CCNNNNNNNGG	8 cut(s)	299, 326, 672, 1062, 1359, 1370, 1391, 1514
BsmAI	GTCTC	3 cut(s)	495, 1509, 1596
BsmFI	GGGAC	3 cut(s)	73, 347, 362
Bso31I	GGTCTC	1 cut(s)	1509
BsoBI	CYCGRG	2 cut(s)	992, 1112
Bsp120I	GGGCCC	1 cut(s)	989
Bsp1286I	GDGCHC	4 cut(s)	69, 946, 970, 993
Bsp13I	TCCGGA	1 cut(s)	694
Bsp143I	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
Bsp1720I	GCTNAGC	1 cut(s)	45
Bsp19I	CCATGG	1 cut(s)	1564
BspCNI	CTCAG	2 cut(s)	37, 982
BspDI	ATCGAT	1 cut(s)	1356
BspEI	TCCGGA	1 cut(s)	694
BspFNI	CGCG	4 cut(s)	71, 176, 726, 1027
BspHI	TCATGA	1 cut(s)	1438
BspLI	GGNNCC	5 cut(s)	238, 693, 699, 771, 991
BspMI	ACCTGC	1 cut(s)	799
BspPI	GGATC	5 cut(s)	372, 711, 764, 777, 1520
BspTNI	GGTCTC	1 cut(s)	1509
BsrBI	CCGCTC	1 cut(s)	1084
BsrDI	GCAATG	1 cut(s)	483
BsrFI	RCCGGY	4 cut(s)	522, 788, 978, 1427
BsrI	ACTGG	2 cut(s)	35, 632
BssAI	RCCGGY	4 cut(s)	522, 788, 978, 1427
BssMI	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
BssNI	GRCGYC	1 cut(s)	1448
BssT1I	CCWWGG	6 cut(s)	78, 240, 277, 854, 1061, 1564
Bst2UI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
Bst4CI	ACNGT	7 cut(s)	22, 272, 302, 386, 447, 1229, 1237
Bst6I	CTCTTC	2 cut(s)	48, 498
BstACI	GRCGYC	1 cut(s)	1448
BstBAI	YACGTR	2 cut(s)	1271, 1495
BstC8I	GCNNGC	7 cut(s)	69, 416, 533, 648, 790, 956, 1002
BstDEI	CTNAG	2 cut(s)	45, 969
BstDSI	CCRYGG	3 cut(s)	1025, 1529, 1564
BstF5I	GGATG	1 cut(s)	662
BstFNI	CGCG	4 cut(s)	71, 176, 726, 1027
BstH2I	RGCGCY	2 cut(s)	126, 250
BstHHI	GCGC	3 cut(s)	125, 225, 249
BstKTI	GATC	8 cut(s)	380, 442, 706, 772, 1111, 1183, 1258, 1528
BstMAI	GTCTC	3 cut(s)	495, 1509, 1596
BstMBI	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
BstMCI	CGRYCG	4 cut(s)	75, 274, 304, 1031
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 102, 122, 182, 282, 997, 1081, 1424
BstNI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BstNSI	RCATGY	1 cut(s)	1004
BstSCI	CCNGG	7 cut(s)	11, 319, 991, 992, 1363, 1384, 1405
BstSFI	CTRYAG	3 cut(s)	231, 501, 1283
BstSLI	GKGCMC	2 cut(s)	946, 993
BstSNI	TACGTA	1 cut(s)	1495
BstUI	CGCG	4 cut(s)	71, 176, 726, 1027
BstV1I	GCAGC	2 cut(s)	857, 984
BstV2I	GAAGAC	3 cut(s)	824, 929, 1404
BstX2I	RGATCY	3 cut(s)	377, 769, 1525
BstYI	RGATCY	3 cut(s)	377, 769, 1525
BstZI	CGGCCG	2 cut(s)	72, 1028
Bsu15I	ATCGAT	1 cut(s)	1356
BsuTUI	ATCGAT	1 cut(s)	1356
BtgI	CCRYGG	3 cut(s)	1025, 1529, 1564
BtgZI	GCGATG	1 cut(s)	789
BtsCI	GGATG	1 cut(s)	662
BtsIMutI	CAGTG	1 cut(s)	1234
BveI	ACCTGC	1 cut(s)	799
Cac8I	GCNNGC	7 cut(s)	69, 416, 533, 648, 790, 956, 1002
CaiI	CAGNNNCTG	1 cut(s)	1370
CciI	TCATGA	1 cut(s)	1438
CciNI	GCGGCCGC	1 cut(s)	72
CfoI	GCGC	3 cut(s)	125, 225, 249
Cfr10I	RCCGGY	4 cut(s)	522, 788, 978, 1427
Cfr13I	GGNCC	8 cut(s)	33, 236, 413, 593, 692, 989, 990, 1425
Cfr42I	CCGCGG	1 cut(s)	1028
Cfr9I	CCCGGG	1 cut(s)	992
ClaI	ATCGAT	1 cut(s)	1356
CseI	GACGC	2 cut(s)	732, 1456
Csp6I	GTAC	4 cut(s)	18, 268, 312, 1280
CspAI	ACCGGT	1 cut(s)	522
CviAII	CATG	4 cut(s)	1001, 1439, 1565, 1606
CviQI	GTAC	4 cut(s)	18, 268, 312, 1280
DdeI	CTNAG	2 cut(s)	45, 969
DpnI	GATC	8 cut(s)	379, 441, 705, 771, 1110, 1182, 1257, 1527
DpnII	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
DrdI	GACNNNNNNGTC	1 cut(s)	487
DriI	GACNNNNNGTC	2 cut(s)	728, 1555
DseDI	GACNNNNNNGTC	1 cut(s)	487
EaeI	YGGCCR	2 cut(s)	72, 1028
EagI	CGGCCG	2 cut(s)	72, 1028
Eam1104I	CTCTTC	2 cut(s)	48, 498
Eam1105I	GACNNNNNGTC	2 cut(s)	728, 1555
EarI	CTCTTC	2 cut(s)	48, 498
EciI	GGCGGA	3 cut(s)	470, 735, 1114
Ecl136II	GAGCTC	1 cut(s)	67
EclXI	CGGCCG	2 cut(s)	72, 1028
Eco105I	TACGTA	1 cut(s)	1495
Eco130I	CCWWGG	6 cut(s)	78, 240, 277, 854, 1061, 1564
Eco147I	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
Eco24I	GRGCYC	2 cut(s)	69, 993
Eco31I	GGTCTC	1 cut(s)	1509
Eco47I	GGWCC	1 cut(s)	692
Eco47III	AGCGCT	1 cut(s)	248
Eco52I	CGGCCG	2 cut(s)	72, 1028
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	1005, 1398
Eco88I	CYCGRG	2 cut(s)	992, 1112
EcoICRI	GAGCTC	1 cut(s)	67
EcoO109I	RGGNCCY	2 cut(s)	236, 989
EcoRI	GAATTC	1 cut(s)	158
EcoRII	CCWGG	4 cut(s)	11, 1363, 1384, 1405
EcoT14I	CCWWGG	6 cut(s)	78, 240, 277, 854, 1061, 1564
EcoT38I	GRGCYC	2 cut(s)	69, 993
ErhI	CCWWGG	6 cut(s)	78, 240, 277, 854, 1061, 1564
FaeI	CATG	4 cut(s)	1004, 1442, 1568, 1609
FaiI	YATR	7 cut(s)	528, 587, 1002, 1277, 1440, 1566, 1607
FalI	AAGNNNNNCTT	2 cut(s)	943, 975
FaqI	GGGAC	3 cut(s)	73, 347, 362
FatI	CATG	4 cut(s)	1000, 1438, 1564, 1605
FauI	CCCGC	6 cut(s)	190, 278, 323, 338, 423, 1077
FbaI	TGATCA	1 cut(s)	1255
FblI	GTMKAC	2 cut(s)	732, 1267
Fnu4HI	GCNGC	9 cut(s)	36, 72, 75, 412, 592, 846, 998, 1025, 1028
FokI	GGATG	1 cut(s)	649
FriOI	GRGCYC	2 cut(s)	69, 993
Fsp4HI	GCNGC	9 cut(s)	36, 72, 75, 412, 592, 846, 998, 1025, 1028
FspBI	CTAG	6 cut(s)	423, 606, 657, 855, 1479, 1536
GlaI	GCGC	3 cut(s)	124, 224, 248
GluI	GCNGC	9 cut(s)	36, 72, 75, 412, 592, 846, 998, 1025, 1028
GsaI	CCCAGC	5 cut(s)	105, 555, 599, 712, 876
GsuI	CTGGAG	3 cut(s)	920, 1097, 1139
HaeII	RGCGCY	2 cut(s)	126, 250
HapII	CCGG	9 cut(s)	320, 510, 523, 695, 701, 789, 979, 993, 1428
HgaI	GACGC	2 cut(s)	732, 1456
HhaI	GCGC	3 cut(s)	125, 225, 249
Hin1I	GRCGYC	1 cut(s)	1448
Hin1II	CATG	4 cut(s)	1004, 1442, 1568, 1609
Hin6I	GCGC	3 cut(s)	123, 223, 247
HinP1I	GCGC	3 cut(s)	123, 223, 247
HincII	GTYRAC	2 cut(s)	466, 733
HindII	GTYRAC	2 cut(s)	466, 733
HinfI	GANTC	8 cut(s)	86, 316, 390, 506, 902, 1208, 1244, 1475
HpaII	CCGG	9 cut(s)	320, 510, 523, 695, 701, 789, 979, 993, 1428
HphI	GGTGA	3 cut(s)	1030, 1061, 1265
Hpy166II	GTNNAC	5 cut(s)	466, 733, 1039, 1225, 1268
Hpy188I	TCNGA	7 cut(s)	157, 433, 679, 777, 842, 907, 985
Hpy188III	TCNNGA	6 cut(s)	209, 695, 1112, 1439, 1479, 1623
Hpy8I	GTNNAC	5 cut(s)	466, 733, 1039, 1225, 1268
Hpy99I	CGWCG	4 cut(s)	215, 398, 401, 940
HpyCH4III	ACNGT	7 cut(s)	22, 272, 302, 386, 447, 1229, 1237
HpyCH4IV	ACGT	2 cut(s)	1270, 1494
HpyCH4V	TGCA	5 cut(s)	265, 1000, 1004, 1240, 1513
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 102, 122, 182, 282, 997, 1081, 1424
HpyF3I	CTNAG	2 cut(s)	45, 969
HpySE526I	ACGT	2 cut(s)	1270, 1494
Hsp92I	GRCGYC	1 cut(s)	1448
Hsp92II	CATG	4 cut(s)	1004, 1442, 1568, 1609
HspAI	GCGC	3 cut(s)	123, 223, 247
Kpn2I	TCCGGA	1 cut(s)	694
KroI	GCCGGC	1 cut(s)	788
KroNI	GCCGGC	1 cut(s)	790
Ksp22I	TGATCA	1 cut(s)	1255
KspI	CCGCGG	1 cut(s)	1028
Kzo9I	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
LmnI	GCTCC	7 cut(s)	64, 244, 655, 842, 1081, 1120, 1596
Lsp1109I	GCAGC	2 cut(s)	857, 984
LweI	GCATC	3 cut(s)	210, 1042, 1132
MaeI	CTAG	6 cut(s)	423, 606, 657, 855, 1479, 1536
MaeII	ACGT	2 cut(s)	1270, 1494
MaeIII	GTNAC	1 cut(s)	1430
MalI	GATC	8 cut(s)	379, 441, 705, 771, 1110, 1182, 1257, 1527
MbiI	CCGCTC	1 cut(s)	1084
MboI	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
MfeI	CAATTG	1 cut(s)	469
MflI	RGATCY	3 cut(s)	377, 769, 1525
MhlI	GDGCHC	4 cut(s)	69, 946, 970, 993
MluCI	AATT	7 cut(s)	158, 469, 680, 758, 909, 1298, 1319
MlyI	GAGTC	3 cut(s)	95, 911, 1253
MmeI	TCCRAC	2 cut(s)	864, 885
MroI	TCCGGA	1 cut(s)	694
MroNI	GCCGGC	1 cut(s)	788
MroXI	GAANNNNTTC	1 cut(s)	836
MslI	CAYNNNNRTG	1 cut(s)	1005
MspA1I	CMGCKG	1 cut(s)	1027
MspI	CCGG	9 cut(s)	320, 510, 523, 695, 701, 789, 979, 993, 1428
MspR9I	CCNGG	7 cut(s)	13, 321, 993, 994, 1365, 1386, 1407
MunI	CAATTG	1 cut(s)	469
MvaI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
MvnI	CGCG	4 cut(s)	71, 176, 726, 1027
MwoI	GCNNNNNNNGC	8 cut(s)	41, 102, 122, 182, 282, 997, 1081, 1424
NaeI	GCCGGC	1 cut(s)	790
NciI	CCSGG	3 cut(s)	321, 993, 994
NcoI	CCATGG	1 cut(s)	1564
NdeII	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
NgoMIV	GCCGGC	1 cut(s)	788
NlaIII	CATG	4 cut(s)	1004, 1442, 1568, 1609
NlaIV	GGNNCC	5 cut(s)	238, 693, 699, 771, 991
NmeAIII	GCCGAG	2 cut(s)	64, 85
NotI	GCGGCCGC	1 cut(s)	72
NspI	RCATGY	1 cut(s)	1004
PaeI	GCATGC	1 cut(s)	1004
PaeR7I	CTCGAG	1 cut(s)	1112
PagI	TCATGA	1 cut(s)	1438
PasI	CCCWGGG	1 cut(s)	12
PceI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
PcsI	WCGNNNNNNNCGW	3 cut(s)	390, 789, 932
PdiI	GCCGGC	1 cut(s)	790
PdmI	GAANNNNTTC	1 cut(s)	836
PfeI	GAWTC	5 cut(s)	316, 390, 506, 1208, 1475
PflFI	GACNNNGTC	1 cut(s)	1450
PflMI	CCANNNNNTGG	1 cut(s)	1370
PinAI	ACCGGT	1 cut(s)	522
PkrI	GCNGC	9 cut(s)	37, 73, 76, 413, 593, 847, 999, 1026, 1029
PleI	GAGTC	3 cut(s)	94, 910, 1252
PpsI	GAGTC	3 cut(s)	94, 910, 1252
Ppu21I	YACGTR	2 cut(s)	1271, 1495
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	4 cut(s)	11, 1363, 1384, 1405
PspFI	CCCAGC	5 cut(s)	101, 551, 595, 708, 872
PspGI	CCWGG	4 cut(s)	11, 1363, 1384, 1405
PspN4I	GGNNCC	5 cut(s)	238, 693, 699, 771, 991
PspOMI	GGGCCC	1 cut(s)	989
PspPI	GGNCC	8 cut(s)	33, 236, 413, 593, 692, 989, 990, 1425
PstNI	CAGNNNCTG	1 cut(s)	1370
PsuI	RGATCY	3 cut(s)	377, 769, 1525
PsyI	GACNNNGTC	1 cut(s)	1450
RsaI	GTAC	4 cut(s)	19, 269, 313, 1281
RsaNI	GTAC	4 cut(s)	18, 268, 312, 1280
RseI	CAYNNNNRTG	1 cut(s)	1005
SacI	GAGCTC	1 cut(s)	69
SacII	CCGCGG	1 cut(s)	1028
SalI	GTCGAC	1 cut(s)	731
SatI	GCNGC	9 cut(s)	36, 72, 75, 412, 592, 846, 998, 1025, 1028
Sau3AI	GATC	8 cut(s)	377, 439, 703, 769, 1108, 1180, 1255, 1525
Sau96I	GGNCC	8 cut(s)	33, 236, 413, 593, 692, 989, 990, 1425
ScaI	AGTACT	1 cut(s)	1281
SchI	GAGTC	3 cut(s)	95, 911, 1253
ScrFI	CCNGG	7 cut(s)	13, 321, 993, 994, 1365, 1386, 1407
SduI	GDGCHC	4 cut(s)	69, 946, 970, 993
SfaNI	GCATC	3 cut(s)	210, 1042, 1132
SfcI	CTRYAG	3 cut(s)	231, 501, 1283
Sfr274I	CTCGAG	1 cut(s)	1112
Sfr303I	CCGCGG	1 cut(s)	1028
SgrBI	CCGCGG	1 cut(s)	1028
SinI	GGWCC	1 cut(s)	692
SlaI	CTCGAG	1 cut(s)	1112
SmaI	CCCGGG	1 cut(s)	994
SmiMI	CAYNNNNRTG	1 cut(s)	1005
SmlI	CTYRAG	3 cut(s)	949, 1112, 1199
SmoI	CTYRAG	3 cut(s)	949, 1112, 1199
SnaBI	TACGTA	1 cut(s)	1495
SphI	GCATGC	1 cut(s)	1004
SrfI	GCCCGGGC	1 cut(s)	994
Sse9I	AATT	7 cut(s)	158, 469, 680, 758, 909, 1298, 1319
SseBI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
SspMI	CTAG	6 cut(s)	423, 606, 657, 855, 1479, 1536
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
StyD4I	CCNGG	7 cut(s)	11, 319, 991, 992, 1363, 1384, 1405
StyI	CCWWGG	6 cut(s)	78, 240, 277, 854, 1061, 1564
TaaI	ACNGT	7 cut(s)	22, 272, 302, 386, 447, 1229, 1237
TaiI	ACGT	2 cut(s)	1273, 1497
TaqI	TCGA	7 cut(s)	393, 732, 783, 850, 962, 1113, 1356
TasI	AATT	7 cut(s)	158, 469, 680, 758, 909, 1298, 1319
TatI	WGTACW	1 cut(s)	1279
TauI	GCSGC	7 cut(s)	38, 74, 77, 414, 594, 1027, 1030
TfiI	GAWTC	5 cut(s)	316, 390, 506, 1208, 1475
TscAI	CASTG	1 cut(s)	1234
TseI	GCWGC	2 cut(s)	845, 997
TspDTI	ATGAA	2 cut(s)	674, 1427
TspGWI	ACGGA	2 cut(s)	1229, 1518
TspMI	CCCGGG	1 cut(s)	992
TspRI	CASTG	1 cut(s)	1234
Tth111I	GACNNNGTC	1 cut(s)	1450
Van91I	CCANNNNNTGG	1 cut(s)	1370
VpaK11BI	GGWCC	1 cut(s)	692
XapI	RAATTY	3 cut(s)	158, 680, 1319
XbaI	TCTAGA	1 cut(s)	1478
XceI	RCATGY	1 cut(s)	1004
XcmI	CCANNNNNNNNNTGG	1 cut(s)	763
XhoI	CTCGAG	1 cut(s)	1112
XmaI	CCCGGG	1 cut(s)	992
XmaJI	CCTAGG	1 cut(s)	854
XmiI	GTMKAC	2 cut(s)	732, 1267
XmnI	GAANNNNTTC	1 cut(s)	836
XspI	CTAG	6 cut(s)	423, 606, 657, 855, 1479, 1536
ZrmI	AGTACT	1 cut(s)	1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.