pycom16g10570

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016314
Physical Location & Seq
Forward (+)
7245995 .. 7248446
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGGATTTTTGGTCCCCTTCTCTCGCCGTCGACGACGAGTTCGAGAAGCTCGCCATGAGAATTAACCCCCCGAGGGTTACAGTTGATAATGCATCAAGCAGGAAAGCCACTCTGATTAAGGTGGACAGTGCAAATAAGCGTGGGAGTTTGCTGGAATTGGTTCAGGTTCTGAATGATTTGAACCTCATTATAAGAAGAGCTTATATTTCTTCTGATGGGGAATGGTTCATGGATGTTTTTCATGTCACTGATCAACAGGGGAATAAGCTTTCTGACGACGGAATCGCTGAATGCATTCAACAGTCACTGGGACCAGCAGGACGAAGCTTCCCTTCTTTGAGAAGGTCTGTGGGTGTCCAAGCTGCCACAGAACACACTACTATTGAATTGACTGGAAGGGACAGGCCAGGCTTGCTTTCTGAAGTTTTTGCTGTTCTTTCGGACCTTAAATGCAATGTGGTTGCCGGAGAAGTCTGGACTCACAATTCAAGAATGGCATCAGTTGTGTACATCACTGACGAGGCAACTGGGAGACCCATTGATGATCCCGAACATCTTGCCAAGATCAAACAGCTTCTCCTTTATGTGCTTAAGGGGAACAGAGATAAGCGCAGTGCCAACACCGCTGTTTCTGTTGGTTCCACACATAAGGAGAGGCGGCTTCACCAGATGATGTATGCTGATCGTGATTACGACATGGATGATGCTGATTGTGGATCAGCCAGTGATCGAAGCAAACCACTTGTCACTGTTGAAAATTGTGTCGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCGGACCGTCCTAAGTTGCTCTTTGACACGGTGTGCACCTTAACAGATATGGAGTATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGGCATACCGACGGCTGCCCTATTAGTTCTGAAGGAGAAAGGCAGAGGGTTATTCATTGTTTGGAAGCTGCTATTCGAAGACGGACTTCTGAGGGTATAAGACTAGAGCTTTGTGGTGATGACAGAGCCGGCCTTCTTTCTGATGTGACTCGCATATTTAGAGAAAATGGTCTTTCAGTCACCCGAGCTGAGGTTGCAACCAGGGGCTCCCAAGCCGTGAATGTGTTCTACGTGACTGATGCATCTGGAAATCAAGTCAAGAGCGAGACAATAGAAGCAGTTCGGCAAGAGATTGGCTTGACATTCCTGCATGTGCAAGACGACTTCTTTTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTTTGGGTAATCTCCTTCGCATTAGGTCGGAGAAGTTCCTTTACAACCTGGGTTTGATAAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

451

Amino Acids

50.23

Weight (kDa)

5.68

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 38 - 88 4.1e-06 ACT domain
ACT PF01842 126 - 192 6.6e-09 ACT domain
ACT PF01842 340 - 397 3e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AccI GTMKAC 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 2 cut(s) 624, 658
AclWI GGATC 2 cut(s) 539, 724
AcuI CTGAAG 3 cut(s) 441, 903, 966
AdeI CACNNNGTG 1 cut(s) 831
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 2 cut(s) 73, 1105
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 6 cut(s) 181, 299, 386, 489, 755, 1248
AjnI CCWGG 3 cut(s) 406, 1115, 1326
AluBI AGCT 9 cut(s) 49, 200, 268, 327, 362, 574, 983, 1024, 1103
AluI AGCT 9 cut(s) 49, 200, 268, 327, 362, 574, 983, 1024, 1103
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 2 cut(s) 526, 1175
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 2 cut(s) 539, 724
AlwNI CAGNNNCTG 2 cut(s) 169, 307
Ama87I CYCGRG 2 cut(s) 70, 1098
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 2 cut(s) 404, 1045
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 3 cut(s) 362, 930, 983
ArsI GACNNNNNNTTYG 6 cut(s) 23, 55, 729, 761, 1008, 1040
Asp700I GAANNNNTTC 4 cut(s) 159, 294, 1139, 1194
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 5 cut(s) 12, 311, 442, 802, 887
AsuHPI GGTGA 3 cut(s) 656, 1043, 1087
AsuII TTCGAA 1 cut(s) 991
AvaI CYCGRG 2 cut(s) 70, 1098
AvaII GGWCC 5 cut(s) 12, 311, 442, 802, 887
BaeGI GKGCMC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 1124
BarI GAAGNNNNNNTAC 2 cut(s) 1304, 1336
BbsI GAAGAC 1 cut(s) 1000
Bbv12I GWGCWC 1 cut(s) 836
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 349, 917, 970
BccI CCATC 1 cut(s) 209
BceAI ACGGC 3 cut(s) 11, 943, 1115
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 3 cut(s) 408, 1117, 1328
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 526, 1175
BfaI CTAG 1 cut(s) 1019
BfrI CTTAAG 1 cut(s) 590
BisI GCNGC 4 cut(s) 363, 659, 931, 984
BlsI GCNGC 4 cut(s) 364, 660, 932, 985
Bme1390I CCNGG 3 cut(s) 408, 1117, 1328
Bme18I GGWCC 5 cut(s) 12, 311, 442, 802, 887
BmeT110I CYCGRG 2 cut(s) 70, 1098
BmgT120I GGNCC 5 cut(s) 12, 311, 442, 802, 887
BmiI GGNNCC 4 cut(s) 14, 312, 640, 1123
BmrFI CCNGG 3 cut(s) 408, 1117, 1328
BmrI ACTGGG 2 cut(s) 317, 537
BmsI GCATC 5 cut(s) 101, 506, 694, 1144, 1166
BmuI ACTGGG 2 cut(s) 317, 537
BpiI GAAGAC 1 cut(s) 1000
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 1 cut(s) 991
BpuEI CTTGAG 1 cut(s) 811
BsaAI YACGTR 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 3 cut(s) 71, 1116, 1327
BsaWI WCCGGW 1 cut(s) 799
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 1105
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 4 cut(s) 312, 397, 532, 723
Bse3DI GCAATG 1 cut(s) 460
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 3 cut(s) 408, 1117, 1328
BseDI CCNNGG 3 cut(s) 71, 1116, 1327
BseGI GGATG 2 cut(s) 238, 706
BseLI CCNNNNNNNGG 2 cut(s) 73, 1105
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 2 cut(s) 996, 1095
BseNI ACTGG 4 cut(s) 312, 397, 532, 723
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 3 cut(s) 349, 917, 970
BshFI GGCC 2 cut(s) 406, 1047
BsiHKAI GWGCWC 1 cut(s) 836
BsiHKCI CYCGRG 2 cut(s) 70, 1098
BsiSI CCGG 3 cut(s) 465, 800, 1044
BslFI GGGAC 2 cut(s) 324, 413
BslI CCNNNNNNNGG 2 cut(s) 73, 1105
BsmAI GTCTC 2 cut(s) 526, 1175
BsmFI GGGAC 2 cut(s) 324, 413
BsmI GAATGC 2 cut(s) 294, 296
BsnI GGCC 2 cut(s) 406, 1047
Bso31I GGTCTC 1 cut(s) 526
BsoBI CYCGRG 2 cut(s) 70, 1098
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 2 cut(s) 836, 1124
Bsp13I TCCGGA 1 cut(s) 799
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 6 cut(s) 250, 544, 564, 682, 716, 727
BspACI CCGC 2 cut(s) 624, 658
BspANI GGCC 2 cut(s) 406, 1047
BspCNI CTCAG 2 cut(s) 997, 1096
BspEI TCCGGA 1 cut(s) 799
BspLI GGNNCC 4 cut(s) 14, 312, 640, 1123
BspPI GGATC 2 cut(s) 539, 724
BspQI GCTCTTC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 991
BspTI CTTAAG 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 1043
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 4 cut(s) 312, 397, 532, 723
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 3 cut(s) 71, 1116, 1327
BssMI GATC 6 cut(s) 250, 544, 564, 682, 716, 727
Bst2UI CCWGG 3 cut(s) 408, 1117, 1328
Bst4CI ACNGT 8 cut(s) 82, 128, 303, 751, 781, 806, 829, 874
Bst6I CTCTTC 1 cut(s) 190
BstAFI CTTAAG 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 507
BstBAI YACGTR 1 cut(s) 1147
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 3 cut(s) 51, 413, 1045
BstDEI CTNAG 3 cut(s) 810, 1005, 1104
BstF5I GGATG 2 cut(s) 238, 706
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 6 cut(s) 253, 547, 567, 685, 719, 730
BstMAI GTCTC 2 cut(s) 526, 1175
BstMBI GATC 6 cut(s) 250, 544, 564, 682, 716, 727
BstMWI GCNNNNNNNGC 3 cut(s) 412, 623, 1109
BstNI CCWGG 3 cut(s) 408, 1117, 1328
BstNSI RCATGY 1 cut(s) 1229
BstSCI CCNGG 3 cut(s) 406, 1115, 1326
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 3 cut(s) 349, 917, 970
BstV2I GAAGAC 1 cut(s) 1000
BsuRI GGCC 2 cut(s) 406, 1047
BtsCI GGATG 2 cut(s) 238, 706
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 7 cut(s) 133, 246, 305, 513, 619, 730, 747
Cac8I GCNNGC 3 cut(s) 51, 413, 1045
CaiI CAGNNNCTG 2 cut(s) 169, 307
CfoI GCGC 1 cut(s) 612
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 5 cut(s) 12, 311, 442, 802, 887
CpoI CGGWCCG 1 cut(s) 802
Csp6I GTAC 1 cut(s) 508
CspI CGGWCCG 1 cut(s) 802
CviAII CATG 6 cut(s) 55, 229, 242, 697, 866, 1226
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 3 cut(s) 810, 1005, 1104
DpnI GATC 6 cut(s) 252, 546, 566, 684, 718, 729
DpnII GATC 6 cut(s) 250, 544, 564, 682, 716, 727
DraIII CACNNNGTG 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 1124
Eco31I GGTCTC 1 cut(s) 526
Eco47I GGWCC 5 cut(s) 12, 311, 442, 802, 887
Eco57I CTGAAG 3 cut(s) 441, 903, 966
Eco88I CYCGRG 2 cut(s) 70, 1098
EcoRII CCWGG 3 cut(s) 406, 1115, 1326
EcoT22I ATGCAT 3 cut(s) 94, 296, 1159
EcoT38I GRGCYC 1 cut(s) 1124
FaeI CATG 6 cut(s) 58, 232, 245, 700, 869, 1229
FalI AAGNNNNNCTT 8 cut(s) 184, 216, 316, 348, 803, 835, 985, 1017
FaqI GGGAC 2 cut(s) 324, 413
FatI CATG 6 cut(s) 54, 228, 241, 696, 865, 1225
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 4 cut(s) 363, 659, 931, 984
FokI GGATG 2 cut(s) 245, 713
FriOI GRGCYC 1 cut(s) 1124
Fsp4HI GCNGC 4 cut(s) 363, 659, 931, 984
FspBI CTAG 1 cut(s) 1019
GlaI GCGC 1 cut(s) 611
GluI GCNGC 4 cut(s) 363, 659, 931, 984
HaeIII GGCC 2 cut(s) 406, 1047
HapII CCGG 3 cut(s) 465, 800, 1044
HhaI GCGC 1 cut(s) 612
Hin1II CATG 6 cut(s) 58, 232, 245, 700, 869, 1229
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 2 cut(s) 266, 325
HinfI GANTC 4 cut(s) 282, 478, 1063, 1275
HpaII CCGG 3 cut(s) 465, 800, 1044
HphI GGTGA 3 cut(s) 656, 1043, 1087
Hpy166II GTNNAC 5 cut(s) 31, 124, 508, 787, 834
Hpy8I GTNNAC 5 cut(s) 31, 124, 508, 787, 834
Hpy99I CGWCG 5 cut(s) 32, 35, 38, 281, 929
HpyAV CCTTC 8 cut(s) 27, 336, 342, 390, 878, 941, 1058, 1304
HpyCH4III ACNGT 8 cut(s) 82, 128, 303, 751, 781, 806, 829, 874
HpyCH4IV ACGT 1 cut(s) 1146
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 623, 1109
HpyF3I CTNAG 3 cut(s) 810, 1005, 1104
HpySE526I ACGT 1 cut(s) 1146
Hsp92II CATG 6 cut(s) 58, 232, 245, 700, 869, 1229
HspAI GCGC 1 cut(s) 610
Kpn2I TCCGGA 1 cut(s) 799
KroI GCCGGC 1 cut(s) 1043
KroNI GCCGGC 1 cut(s) 1045
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 6 cut(s) 250, 544, 564, 682, 716, 727
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 1127
Lsp1109I GCAGC 3 cut(s) 349, 917, 970
LweI GCATC 5 cut(s) 101, 506, 694, 1144, 1166
MaeI CTAG 1 cut(s) 1019
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 7 cut(s) 76, 244, 303, 745, 1060, 1093, 1147
MalI GATC 6 cut(s) 252, 546, 566, 684, 718, 729
MboI GATC 6 cut(s) 250, 544, 564, 682, 716, 727
MboII GAAGA 3 cut(s) 201, 207, 1005
MhlI GDGCHC 2 cut(s) 836, 1124
MluCI AATT 5 cut(s) 60, 155, 386, 484, 757
MlyI GAGTC 2 cut(s) 472, 1057
MmeI TCCRAC 1 cut(s) 1287
MnlI CCTC 9 cut(s) 66, 194, 514, 648, 786, 886, 954, 1000, 1099
Mph1103I ATGCAT 3 cut(s) 94, 296, 1159
MroI TCCGGA 1 cut(s) 799
MroNI GCCGGC 1 cut(s) 1043
MroXI GAANNNNTTC 4 cut(s) 159, 294, 1139, 1194
MseI TTAA 5 cut(s) 63, 117, 447, 591, 839
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 3 cut(s) 465, 800, 1044
MspR9I CCNGG 3 cut(s) 408, 1117, 1328
Mva1269I GAATGC 2 cut(s) 294, 296
MvaI CCWGG 3 cut(s) 408, 1117, 1328
MwoI GCNNNNNNNGC 3 cut(s) 412, 623, 1109
NaeI GCCGGC 1 cut(s) 1045
NdeII GATC 6 cut(s) 250, 544, 564, 682, 716, 727
NgoMIV GCCGGC 1 cut(s) 1043
NlaIII CATG 6 cut(s) 58, 232, 245, 700, 869, 1229
NlaIV GGNNCC 4 cut(s) 14, 312, 640, 1123
NmuCI GTSAC 6 cut(s) 244, 303, 745, 1060, 1093, 1147
NsiI ATGCAT 3 cut(s) 94, 296, 1159
NspI RCATGY 1 cut(s) 1229
NspV TTCGAA 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 39
PctI GAATGC 2 cut(s) 294, 296
PdiI GCCGGC 1 cut(s) 1045
PdmI GAANNNNTTC 4 cut(s) 159, 294, 1139, 1194
PfeI GAWTC 2 cut(s) 282, 1275
PkrI GCNGC 4 cut(s) 364, 660, 932, 985
PleI GAGTC 2 cut(s) 472, 1057
PpsI GAGTC 2 cut(s) 472, 1057
Ppu21I YACGTR 1 cut(s) 1147
PsiI TTATAA 1 cut(s) 191
Psp6I CCWGG 3 cut(s) 406, 1115, 1326
PspGI CCWGG 3 cut(s) 406, 1115, 1326
PspN4I GGNNCC 4 cut(s) 14, 312, 640, 1123
PspPI GGNCC 5 cut(s) 12, 311, 442, 802, 887
PstNI CAGNNNCTG 2 cut(s) 169, 307
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
Rsr2I CGGWCCG 1 cut(s) 802
RsrII CGGWCCG 1 cut(s) 802
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 190
SaqAI TTAA 5 cut(s) 63, 117, 447, 591, 839
SatI GCNGC 4 cut(s) 363, 659, 931, 984
Sau3AI GATC 6 cut(s) 250, 544, 564, 682, 716, 727
Sau96I GGNCC 5 cut(s) 12, 311, 442, 802, 887
SchI GAGTC 2 cut(s) 472, 1057
ScrFI CCNGG 3 cut(s) 408, 1117, 1328
SduI GDGCHC 2 cut(s) 836, 1124
SfaNI GCATC 5 cut(s) 101, 506, 694, 1144, 1166
SfuI TTCGAA 1 cut(s) 991
SgrDI CGTCGACG 1 cut(s) 29
SinI GGWCC 5 cut(s) 12, 311, 442, 802, 887
SmlI CTYRAG 2 cut(s) 590, 790
SmoI CTYRAG 2 cut(s) 590, 790
Sse9I AATT 5 cut(s) 60, 155, 386, 484, 757
SsiI CCGC 2 cut(s) 624, 658
SspI AATATT 1 cut(s) 908
SspMI CTAG 1 cut(s) 1019
StyD4I CCNGG 3 cut(s) 406, 1115, 1326
TaaI ACNGT 8 cut(s) 82, 128, 303, 751, 781, 806, 829, 874
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 5 cut(s) 30, 42, 730, 765, 991
TasI AATT 5 cut(s) 60, 155, 386, 484, 757
TatI WGTACW 1 cut(s) 507
TauI GCSGC 1 cut(s) 661
TfiI GAWTC 2 cut(s) 282, 1275
Tru1I TTAA 5 cut(s) 63, 117, 447, 591, 839
Tru9I TTAA 5 cut(s) 63, 117, 447, 591, 839
TscAI CASTG 7 cut(s) 133, 253, 312, 520, 619, 730, 754
TseFI GTSAC 6 cut(s) 244, 303, 745, 1060, 1093, 1147
TseI GCWGC 3 cut(s) 362, 930, 983
Tsp45I GTSAC 6 cut(s) 244, 303, 745, 1060, 1093, 1147
TspDTI ATGAA 3 cut(s) 217, 230, 959
TspGWI ACGGA 2 cut(s) 294, 1012
TspRI CASTG 7 cut(s) 133, 253, 312, 520, 619, 730, 754
Vha464I CTTAAG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 5 cut(s) 12, 311, 442, 802, 887
XceI RCATGY 1 cut(s) 1229
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 4 cut(s) 159, 294, 1139, 1194
XspI CTAG 1 cut(s) 1019
Zsp2I ATGCAT 3 cut(s) 94, 296, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.